Lag0022677 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0022677 vs. NCBI nr
Match: XP_038904500.1 (disease resistance protein RGA2-like, partial [Benincasa hispida])

HSP 1 Score: 533.5 bits (1373), Expect = 2.0e-147
Identity = 284/437 (64.99%), Postives = 330/437 (75.51%), Query Frame = 0

Query: 1   MLYNLQTLKLGNMTSSL-KNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVE 60
           +LYNLQTLKL N+ S L KNLRKL+NLRHLEF   + I+++PSH+GKLIHLQTLS FAV 
Sbjct: 49  LLYNLQTLKLENIKSGLSKNLRKLINLRHLEFYRIHDIQQIPSHIGKLIHLQTLSEFAVG 108

Query: 61  FEKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWN--SREGE 120
            EKGCKIEELG LKNL+  L LTNLE V+SKEEA AAKLV+KKNL  L+FHW    +EGE
Sbjct: 109 IEKGCKIEELGPLKNLKGALNLTNLEKVQSKEEARAAKLVEKKNLHHLLFHWTFFYKEGE 168

Query: 121 YEKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQ 180
           Y+K N ++VLEGLQPHKNLQSL I  F G+VLPN  FVE LV+I L +C++C+VLPMLGQ
Sbjct: 169 YDKYNKVQVLEGLQPHKNLQSLNISGFRGEVLPNSTFVENLVDIHLFNCKRCEVLPMLGQ 228

Query: 181 LPNLKKLSIFSMDDVRSIGNEFYGIDSNQTD--SFPQLKKLCIYWMKNLEQWDEAPLVPN 240
           LPNLKKL I  +D +RSIGNEFYGIDSNQ +  +FPQLKK  I  MKNLE+WDE  + PN
Sbjct: 229 LPNLKKLEISLLDSLRSIGNEFYGIDSNQRNYFAFPQLKKFYIDGMKNLEKWDEL-ITPN 288

Query: 241 LFGSLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLER 300
           LFGSL+   I+GC+KLTKLP GIE C SI+YL I +CPNLMLNVQNLHNLY LDING++R
Sbjct: 289 LFGSLKNFSILGCDKLTKLPGGIESCQSIEYLSIHQCPNLMLNVQNLHNLYRLDINGMKR 348

Query: 301 LPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLIELELYNDESNIATQLPEQLEHLTAL 360
           LPEGL   TNLKE+ I G  QNY+ S                  N ATQLP+QL+HLT L
Sbjct: 349 LPEGLGGLTNLKELVISGRMQNYESS-----------------DNAATQLPQQLQHLTNL 408

Query: 361 KVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFDGCPQ 420
           +VL I DFD I+ LPEWL NLTSL TL F  CKNL++LPSR+A LCLTKL+ L    CP+
Sbjct: 409 EVLMIADFDSIEALPEWLGNLTSLRTLSFLDCKNLKELPSREAMLCLTKLDDLLVFRCPK 467

Query: 421 LLHG----ERAKLSHLP 429
           LL G    E  KLSHLP
Sbjct: 469 LLVGEGDQEGPKLSHLP 467

BLAST of Lag0022677 vs. NCBI nr
Match: XP_031741283.1 (disease resistance protein RGA2-like [Cucumis sativus])

HSP 1 Score: 518.1 bits (1333), Expect = 8.8e-143
Identity = 278/446 (62.33%), Postives = 337/446 (75.56%), Query Frame = 0

Query: 2   LYNLQTLKLGNMTSSL-KNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVEF 61
           LYNLQTLKLG +   L KNLR LVNLRHLEF + + + +MPSHMG +IHLQTLS F V  
Sbjct: 477 LYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFVVGL 536

Query: 62  EKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSR---EGE 121
           EKGCKI+ELG LK+L+ TL L NL+NV++K+EA+AAKLV+KK LR LIF W       GE
Sbjct: 537 EKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNLYDRGE 596

Query: 122 YEKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQ 181
           Y++ +N +VLEGLQPHKN+QSL I+ F G+VL N +FVE LVEI+L  C +C+VLPMLGQ
Sbjct: 597 YDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLPMLGQ 656

Query: 182 LPNLKKLSIFSMDDVRSIGNEFYGIDSNQTDS--FPQLKKLCIYWMKNLEQWDEAPL-VP 241
           LPNLKKL I SM+ VRSIG+EFYG+D N  +S  FPQL K  I  +K L+QWDEA +   
Sbjct: 657 LPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKLQQWDEATVFAS 716

Query: 242 NLFGSLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLE 301
           N FG L++L + GC +L KLPSG+E C SI+YL I  CPNLMLNVQNL+NLYHLDI GL+
Sbjct: 717 NRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDIRGLK 776

Query: 302 RLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCS-LIELELYNDES--NIATQLPEQLEH 361
           RLP+     TNLK+++I GC QNY+FSPF+HL S L+ELEL +D S  +  TQLP+QL+H
Sbjct: 777 RLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSGSETTQLPQQLQH 836

Query: 362 LTALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFD 421
           LT LKVL I DFD I+VLPEWL NLT L TL F  CKNL++LPSR+A   LTKL+ L  D
Sbjct: 837 LTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAIQRLTKLDDLVID 896

Query: 422 GCPQLLHG----ERAKLSHLPEFVLR 434
           GCP+LL G    ERAKLSHLP   +R
Sbjct: 897 GCPKLLLGEGDQERAKLSHLPSKCVR 922

BLAST of Lag0022677 vs. NCBI nr
Match: XP_004140442.1 (disease resistance protein RGA2 [Cucumis sativus] >KGN50811.1 hypothetical protein Csa_018865 [Cucumis sativus])

HSP 1 Score: 518.1 bits (1333), Expect = 8.8e-143
Identity = 278/446 (62.33%), Postives = 337/446 (75.56%), Query Frame = 0

Query: 2    LYNLQTLKLGNMTSSL-KNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVEF 61
            LYNLQTLKLG +   L KNLR LVNLRHLEF + + + +MPSHMG +IHLQTLS F V  
Sbjct: 594  LYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFVVGL 653

Query: 62   EKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSR---EGE 121
            EKGCKI+ELG LK+L+ TL L NL+NV++K+EA+AAKLV+KK LR LIF W       GE
Sbjct: 654  EKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNLYDRGE 713

Query: 122  YEKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQ 181
            Y++ +N +VLEGLQPHKN+QSL I+ F G+VL N +FVE LVEI+L  C +C+VLPMLGQ
Sbjct: 714  YDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLPMLGQ 773

Query: 182  LPNLKKLSIFSMDDVRSIGNEFYGIDSNQTDS--FPQLKKLCIYWMKNLEQWDEAPL-VP 241
            LPNLKKL I SM+ VRSIG+EFYG+D N  +S  FPQL K  I  +K L+QWDEA +   
Sbjct: 774  LPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKLQQWDEATVFAS 833

Query: 242  NLFGSLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLE 301
            N FG L++L + GC +L KLPSG+E C SI+YL I  CPNLMLNVQNL+NLYHLDI GL+
Sbjct: 834  NRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDIRGLK 893

Query: 302  RLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCS-LIELELYNDES--NIATQLPEQLEH 361
            RLP+     TNLK+++I GC QNY+FSPF+HL S L+ELEL +D S  +  TQLP+QL+H
Sbjct: 894  RLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSGSETTQLPQQLQH 953

Query: 362  LTALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFD 421
            LT LKVL I DFD I+VLPEWL NLT L TL F  CKNL++LPSR+A   LTKL+ L  D
Sbjct: 954  LTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAIQRLTKLDDLVID 1013

Query: 422  GCPQLLHG----ERAKLSHLPEFVLR 434
            GCP+LL G    ERAKLSHLP   +R
Sbjct: 1014 GCPKLLLGEGDQERAKLSHLPSKCVR 1039

BLAST of Lag0022677 vs. NCBI nr
Match: XP_038885174.1 (disease resistance protein RGA2-like [Benincasa hispida])

HSP 1 Score: 498.0 bits (1281), Expect = 9.4e-137
Identity = 267/440 (60.68%), Postives = 321/440 (72.95%), Query Frame = 0

Query: 2    LYNLQTLKLGNMTSSLKNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVEFE 61
            LYNLQTLKLG++ +  ++LR LV LRHL+F + Y I +MPSHMG+LIHLQTLSGF V FE
Sbjct: 647  LYNLQTLKLGSIENVPRSLRNLVRLRHLDFHDDYNIRQMPSHMGELIHLQTLSGFVVGFE 706

Query: 62   KGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWN---SREGEY 121
            +GCKIEELG LKNL+  L L+NLE VKSKEEAIAAKL  KKNLR+L F W+    REG  
Sbjct: 707  EGCKIEELGNLKNLKGQLRLSNLEQVKSKEEAIAAKLADKKNLRELTFEWSIDFLREG-- 766

Query: 122  EKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQL 181
               N+ EVLEGLQPHKNL SLKI  FGGK LP   FVE L+ + L  C KC+ LP+LGQL
Sbjct: 767  SNYNDSEVLEGLQPHKNLCSLKITNFGGKFLPTATFVENLMFLCLYGCTKCERLPVLGQL 826

Query: 182  PNLKKLSIFSMDDVRSIGNEFYGIDSNQTDSFPQLKKLCIYWMKNLEQW--DEAPLVPNL 241
            PNLK+L I  MD VRSIG+EFYG DSNQ D FP+LKK  + WM NLEQW  + A    N 
Sbjct: 827  PNLKELGICFMDSVRSIGSEFYGNDSNQRDYFPKLKKFDLGWMCNLEQWELEVANNESNH 886

Query: 242  FGSLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLERL 301
            FGSLQ L++  C KL KLP+G+ECC S+  +II  CPNL LNV+ +HNL  L I+ L++L
Sbjct: 887  FGSLQTLKLDRCGKLAKLPNGLECCKSVHDVIISSCPNLTLNVEKMHNLSVLTIDELKKL 946

Query: 302  PEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLIELELYNDESNIATQLPEQLEHLTALK 361
            P+GLA   NLK + I GC Q+YD+SPF+HL SL +L L ND    ATQLP+QL+HLTALK
Sbjct: 947  PKGLARLPNLKTMMITGCIQDYDYSPFLHLPSLTKLYL-NDGLRNATQLPQQLQHLTALK 1006

Query: 362  VLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFDGCPQL 421
            +L+IE+F GI+VLPEWL+ LT LETL   RCKNL+QLPSR+  LCLTKL      GCP L
Sbjct: 1007 ILAIENFHGIEVLPEWLKKLTCLETLDLVRCKNLKQLPSRETMLCLTKLKDFKVMGCPSL 1066

Query: 422  LHG-----ERAKLSHLPEFV 432
            L G     E AK + +P ++
Sbjct: 1067 LLGGQADREGAKFTDIPAYL 1083

BLAST of Lag0022677 vs. NCBI nr
Match: XP_004136783.2 (disease resistance protein RGA2 [Cucumis sativus])

HSP 1 Score: 491.9 bits (1265), Expect = 6.7e-135
Identity = 271/439 (61.73%), Postives = 329/439 (74.94%), Query Frame = 0

Query: 1    MLYNLQTLKLGNMTSSLKNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVEF 60
            +LYNLQTLKLG  +   KNLRKLVNLRHLEF + +    MPS MG LIHLQ+LSGF V F
Sbjct: 602  LLYNLQTLKLG-QSGLPKNLRKLVNLRHLEF-KMFGDTAMPSDMGNLIHLQSLSGFLVGF 661

Query: 61   EKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLR--KLIFHWNSREGEY 120
            EKGCKIEELG LKNL+  L LTNL  V++K+EA+AAKLV+KKNLR   L F    + GE 
Sbjct: 662  EKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGED 721

Query: 121  EKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQL 180
            ++   ++VLEGLQPHKNLQSL+I  F GKVLP G+FVE LV+I+L   E+C+VLPMLGQL
Sbjct: 722  DEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQL 781

Query: 181  PNLKKLSIFSMDDVRSIGNEFYGIDSNQTDS--FPQLKKLCIYWMKNLEQWDEAPLV--P 240
            PNLK+L I  M+ VRSIGNEFYG+DS+  +S  FPQLKKL IY M NLEQWDEA +V   
Sbjct: 782  PNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLES 841

Query: 241  NLFGSLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLE 300
            NLFG L+++ I  C  L KLPSG+E C+S++YL IR C NLMLNVQNLH LYHL+I+GL+
Sbjct: 842  NLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLK 901

Query: 301  RLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCS-LIELELYNDESNIATQLPEQLEHLT 360
            RLP+G+   T LKE+KI GC QNY+FS  +HL S L+ELEL     ++ TQLP+QL+HLT
Sbjct: 902  RLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQHLT 961

Query: 361  ALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFDGC 420
             L+VL I  FD I+ LPEW+ NL SL+TL+   C  L++LPSR+A L LTKL +L    C
Sbjct: 962  NLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIFEC 1021

Query: 421  PQLLHG----ERAKLSHLP 429
            P+LL G    ERAKLSHLP
Sbjct: 1022 PKLLVGEGDQERAKLSHLP 1038

BLAST of Lag0022677 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.4e-34
Identity = 136/412 (33.01%), Postives = 196/412 (47.57%), Query Frame = 0

Query: 2    LYNLQTLKLGNMTSSLKNL----RKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFA 61
            +YNLQTL L +  SSLK L      L+NLR+L+      + +MP   G+L  LQTL+ F 
Sbjct: 624  MYNLQTLLL-SYCSSLKELPTDISNLINLRYLDLIGT-KLRQMPRRFGRLKSLQTLTTFF 683

Query: 62   VEFEKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSREGE 121
            V    G +I ELG L +L   L +  L+ V    +A  A L  KK+LR++ F W +    
Sbjct: 684  VSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTGSSS 743

Query: 122  YEK-------CNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGV---FVEILVEIKLRSCE 181
             E         N  EV E L+PH++++ L I+ + G+  P+ +       +V I+LR C+
Sbjct: 744  SENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQ 803

Query: 182  KCKVLPMLGQLPNLKKLSIFSMDDVRSIGNEFYGIDSNQTDSFPQ-LKKLCIYWMKNLEQ 241
             C  LP LGQLP LK+L I  M  ++SIG +FY  D    D   Q  + L      NL  
Sbjct: 804  YCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPD 863

Query: 242  WDEAPLV----PNLFGSLQQLEIVGCEKLT-KLPSGIECCNSIKYLIIRKCPNLMLNVQN 301
            W E   V     +LF SL++L I+ C +LT  LP+ +    S+  L I KC   +L+ Q 
Sbjct: 864  WQEWLDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLP---SLISLHIYKCG--LLDFQP 923

Query: 302  LHNLYHLDINGLERLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLIELELYNDESNI 361
             H+ Y                  NL+ + IK         P  H  +L +LE+    S  
Sbjct: 924  DHHEY---------------SYRNLQTLSIKSSCDTLVKFPLNHFANLDKLEVDQCTSLY 983

Query: 362  ATQLP-EQLEHLTALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQ 393
            + +L  E L    AL+ L I D   + +LP+ L  L     +    C+ LRQ
Sbjct: 984  SLELSNEHLRGPNALRNLRINDCQNLQLLPK-LNALPQNLQVTITNCRYLRQ 1012

BLAST of Lag0022677 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.9e-32
Identity = 137/446 (30.72%), Postives = 212/446 (47.53%), Query Frame = 0

Query: 2   LYNLQTLKLGN---MTSSLKNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAV 61
           L NLQTL + N   +    K   KL +LRHL  D    +   P  +G L  L+TL  F V
Sbjct: 572 LQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVD-GCPLTSTPPRIGLLTCLKTLGFFIV 631

Query: 62  EFEKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSREGEY 121
             +KG ++ EL  L NL  ++ +T+LE VK+  +A  A L  K NL+ L   W++     
Sbjct: 632 GSKKGYQLGELKNL-NLCGSISITHLERVKNDTDA-EANLSAKANLQSLSMSWDNDGPNR 691

Query: 122 EKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLP---NGVFVEILVEIKLRSCEKCKVLPML 181
            +   ++VLE L+PH NL+ L+I AFGG   P   N   +E ++ ++++SC+ C  LP  
Sbjct: 692 YESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPF 751

Query: 182 GQLPNLKKLSIFS-MDDVRSIGNEFYGIDSNQTDSFPQLKKLCIYWMKNLEQWDEAPLVP 241
           G+LP L+ L + +   +V  +  +      +   SFP LKKL I++ ++L+         
Sbjct: 752 GELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSLK--------- 811

Query: 242 NLFGSLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLE 301
              G +++              G E    ++ + I  CP  +     L ++  L+++G  
Sbjct: 812 ---GLMKE-------------EGEEKFPMLEEMAILYCP--LFVFPTLSSVKKLEVHG-N 871

Query: 302 RLPEGLAHQTNLKEIKIKGCRQNYDFS--PFMHLCSLIELEL--YNDESNIATQLPEQLE 361
               GL+  +NL  +       NY  +  P     SL  LE   + D  N+   LP  L 
Sbjct: 872 TNTRGLSSISNLSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKNL-KDLPTSLT 931

Query: 362 HLTALKVLSIEDFDGIDVLPE-WLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLF 421
            L ALK L IE  D ++  PE  LE LTSL  L    CK L+ LP  +    LT L +L 
Sbjct: 932 SLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLP--EGLQHLTALTNLG 983

Query: 422 FDGCPQL-------LHGERAKLSHLP 429
             GCP++       +  +  K++H+P
Sbjct: 992 VSGCPEVEKRCDKEIGEDWHKIAHIP 983

BLAST of Lag0022677 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 8.5e-32
Identity = 131/410 (31.95%), Postives = 192/410 (46.83%), Query Frame = 0

Query: 2   LYNLQTLKLGNMTSSL---KNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAV 61
           L NLQTL L   T      K   KL +LR+L  D    +  MP  +G L  L+TL  F V
Sbjct: 571 LQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVV 630

Query: 62  EFEKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSREGEY 121
             +KG ++ ELG L NL  ++ +++LE VK+ ++A  A L  K NL  L   WN+     
Sbjct: 631 GRKKGYQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSWNNFGPHI 690

Query: 122 EKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLP---NGVFVEILVEIKLRSCEKCKVLPML 181
            +   ++VLE L+PH NL SLKI  F G  LP   N   ++ +V I + +   C  LP  
Sbjct: 691 YESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPF 750

Query: 182 GQLPNLKKLSI-FSMDDVRSIGNEFYGIDSNQTDSFPQLKKLCIYWMKNLEQWDEAPLVP 241
           G LP L+ L + +   DV  +      +D +    FP   ++    ++ L+ WD   L  
Sbjct: 751 GDLPCLESLELHWGSADVEYVEE----VDIDVHSGFP--TRIRFPSLRKLDIWDFGSLKG 810

Query: 242 NL-------FGSLQQLEIVGCEKLTKLPSGIECCNSIKYL---IIRKCPNLMLNVQNLHN 301
            L       F  L+++ I  C  LT L S +    S++     +    P  M   +NL N
Sbjct: 811 LLKKEGEEQFPVLEEMIIHECPFLT-LSSNLRALTSLRICYNKVATSFPEEMF--KNLAN 870

Query: 302 LYHLDI---NGLERLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLIELELYNDESNI 361
           L +L I   N L+ LP  LA    LK +KI+ C            C+L            
Sbjct: 871 LKYLTISRCNNLKELPTSLASLNALKSLKIQLC------------CAL------------ 930

Query: 362 ATQLPEQ-LEHLTALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNL 391
              LPE+ LE L++L  L +E  + +  LPE L++LT+L +L+   C  L
Sbjct: 931 -ESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQL 945

BLAST of Lag0022677 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 133.7 bits (335), Expect = 6.1e-30
Identity = 136/448 (30.36%), Postives = 202/448 (45.09%), Query Frame = 0

Query: 2   LYNLQTLKL---GNMTSSLKNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAV 61
           L NLQTL L    +++   K   KL +LR+L  D    +   P  +G L  L++LS F +
Sbjct: 571 LQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLD-GCSLTSTPPRIGLLTCLKSLSCFVI 630

Query: 62  EFEKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSREGEY 121
              KG ++ EL  L NL  ++ +T L+ VK   +A  A L  K NL  L   W+  +G++
Sbjct: 631 GKRKGHQLGELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSWD-LDGKH 690

Query: 122 EKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEIL---VEIKLRSCEKCKVLPML 181
               + EVLE L+PH NL+ L+I  FGG  LP+ +   +L   V I++R CE C  LP  
Sbjct: 691 R--YDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPF 750

Query: 182 GQLPNLKKLSIFSMDDVRSIGNEFYGIDSNQTDSFPQLKKLCIYWMKNLEQWDEAPLVPN 241
           G+LP L+ L + +        +  Y  D+     FP L+KL I+   NL+   +      
Sbjct: 751 GELPCLESLELHT-----GSADVEYVEDNVHPGRFPSLRKLVIWDFSNLKGLLKME-GEK 810

Query: 242 LFGSLQQLEIVGCEKLTKLPSGIECCNSIKYL-IIRKCPNLMLNVQNLHNLYHLDING-- 301
            F  L+++    C         I   +S+K L +I     ++ ++ NL  L  LDI+   
Sbjct: 811 QFPVLEEMTFYWCPMFV-----IPTLSSVKTLKVIVTDATVLRSISNLRALTSLDISDNV 870

Query: 302 -LERLPEGLAHQ-TNLKEIKIKGCRQNYDFSPFMHLCSLIELELYNDESNIATQLPEQLE 361
               LPE +     NLK +KI   R                            +LP  L 
Sbjct: 871 EATSLPEEMFKSLANLKYLKISFFRN-------------------------LKELPTSLA 930

Query: 362 HLTALKVLSIEDFDGIDVLP-EWLENLTSLETLRFHRCKNLRQLPSRKAKL-CLTKLN-- 421
            L ALK L  E  D ++ LP E ++ LTSL  L    C  L+ LP     L  LT L   
Sbjct: 931 SLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTIT 977

Query: 422 --HLFFDGCPQLLHGERAKLSHLPEFVL 433
              + F  C + +  +  K++H+P   L
Sbjct: 991 QCPIVFKRCERGIGEDWHKIAHIPYLTL 977

BLAST of Lag0022677 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 130.2 bits (326), Expect = 6.7e-29
Identity = 139/421 (33.02%), Postives = 194/421 (46.08%), Query Frame = 0

Query: 2   LYNLQTLKLGNMTSSL---KNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAV 61
           L NLQTL L N  S     K   KL +LR+L  D    +  MP  +G L  L+TL  F V
Sbjct: 580 LQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDH-CPLTSMPPRIGLLTCLKTLGYFVV 639

Query: 62  EFEKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSREGEY 121
              KG ++ EL  L NL   + +T+LE VK+  EA  A L  K NL  L   W+ R   Y
Sbjct: 640 GERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSWD-RPNRY 699

Query: 122 EKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLP---NGVFVEILVEIKLRSCEKCKVLPML 181
           E    ++VLE L+PH NL+ L+I  F G  LP   N   ++ +V I +  CE C  LP  
Sbjct: 700 ES-EEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPF 759

Query: 182 GQLPNLKKLSIFSMDDVRSIGNEFYGIDSNQT-DSFPQLKKLCI---------YWMKNLE 241
           G+LP L+ L +       S+  E+       T   FP L+KL I           MK  E
Sbjct: 760 GELPCLESLEL----QDGSVEVEYVEDSGFLTRRRFPSLRKLHIGGFCNLKGLQRMKGAE 819

Query: 242 QW--------DEAPL-VPNLFGSLQQLEIVGCEKLTKLP--SGIECCNSIKYLIIRKCPN 301
           Q+         + P+ V     S+++LEI G      L   S +    S+K        +
Sbjct: 820 QFPVLEEMKISDCPMFVFPTLSSVKKLEIWGEADAGGLSSISNLSTLTSLKIFSNHTVTS 879

Query: 302 LMLNV-QNLHNLYHLDINGLE---RLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLI 361
           L+  + +NL NL +L ++ LE    LP  LA   NLK + I+ C                
Sbjct: 880 LLEEMFKNLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYC---------------- 939

Query: 362 ELELYNDESNIATQLPEQ-LEHLTALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKN 391
               Y  ES     LPE+ LE L++L  L +E  + +  LPE L++LT+L +L+   C  
Sbjct: 940 ----YALES-----LPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQ 967

BLAST of Lag0022677 vs. ExPASy TrEMBL
Match: A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 4.2e-143
Identity = 278/446 (62.33%), Postives = 337/446 (75.56%), Query Frame = 0

Query: 2    LYNLQTLKLGNMTSSL-KNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVEF 61
            LYNLQTLKLG +   L KNLR LVNLRHLEF + + + +MPSHMG +IHLQTLS F V  
Sbjct: 594  LYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFVVGL 653

Query: 62   EKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSR---EGE 121
            EKGCKI+ELG LK+L+ TL L NL+NV++K+EA+AAKLV+KK LR LIF W       GE
Sbjct: 654  EKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNLYDRGE 713

Query: 122  YEKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQ 181
            Y++ +N +VLEGLQPHKN+QSL I+ F G+VL N +FVE LVEI+L  C +C+VLPMLGQ
Sbjct: 714  YDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLPMLGQ 773

Query: 182  LPNLKKLSIFSMDDVRSIGNEFYGIDSNQTDS--FPQLKKLCIYWMKNLEQWDEAPL-VP 241
            LPNLKKL I SM+ VRSIG+EFYG+D N  +S  FPQL K  I  +K L+QWDEA +   
Sbjct: 774  LPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKLQQWDEATVFAS 833

Query: 242  NLFGSLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLE 301
            N FG L++L + GC +L KLPSG+E C SI+YL I  CPNLMLNVQNL+NLYHLDI GL+
Sbjct: 834  NRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDIRGLK 893

Query: 302  RLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCS-LIELELYNDES--NIATQLPEQLEH 361
            RLP+     TNLK+++I GC QNY+FSPF+HL S L+ELEL +D S  +  TQLP+QL+H
Sbjct: 894  RLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSGSETTQLPQQLQH 953

Query: 362  LTALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFD 421
            LT LKVL I DFD I+VLPEWL NLT L TL F  CKNL++LPSR+A   LTKL+ L  D
Sbjct: 954  LTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAIQRLTKLDDLVID 1013

Query: 422  GCPQLLHG----ERAKLSHLPEFVLR 434
            GCP+LL G    ERAKLSHLP   +R
Sbjct: 1014 GCPKLLLGEGDQERAKLSHLPSKCVR 1039

BLAST of Lag0022677 vs. ExPASy TrEMBL
Match: A0A0A0LBV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 3.3e-135
Identity = 271/439 (61.73%), Postives = 329/439 (74.94%), Query Frame = 0

Query: 1    MLYNLQTLKLGNMTSSLKNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVEF 60
            +LYNLQTLKLG  +   KNLRKLVNLRHLEF + +    MPS MG LIHLQ+LSGF V F
Sbjct: 602  LLYNLQTLKLG-QSGLPKNLRKLVNLRHLEF-KMFGDTAMPSDMGNLIHLQSLSGFLVGF 661

Query: 61   EKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLR--KLIFHWNSREGEY 120
            EKGCKIEELG LKNL+  L LTNL  V++K+EA+AAKLV+KKNLR   L F    + GE 
Sbjct: 662  EKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGED 721

Query: 121  EKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQL 180
            ++   ++VLEGLQPHKNLQSL+I  F GKVLP G+FVE LV+I+L   E+C+VLPMLGQL
Sbjct: 722  DEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQL 781

Query: 181  PNLKKLSIFSMDDVRSIGNEFYGIDSNQTDS--FPQLKKLCIYWMKNLEQWDEAPLV--P 240
            PNLK+L I  M+ VRSIGNEFYG+DS+  +S  FPQLKKL IY M NLEQWDEA +V   
Sbjct: 782  PNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLES 841

Query: 241  NLFGSLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLE 300
            NLFG L+++ I  C  L KLPSG+E C+S++YL IR C NLMLNVQNLH LYHL+I+GL+
Sbjct: 842  NLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLK 901

Query: 301  RLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCS-LIELELYNDESNIATQLPEQLEHLT 360
            RLP+G+   T LKE+KI GC QNY+FS  +HL S L+ELEL     ++ TQLP+QL+HLT
Sbjct: 902  RLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQHLT 961

Query: 361  ALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFDGC 420
             L+VL I  FD I+ LPEW+ NL SL+TL+   C  L++LPSR+A L LTKL +L    C
Sbjct: 962  NLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIFEC 1021

Query: 421  PQLLHG----ERAKLSHLP 429
            P+LL G    ERAKLSHLP
Sbjct: 1022 PKLLVGEGDQERAKLSHLP 1038

BLAST of Lag0022677 vs. ExPASy TrEMBL
Match: A0A5A7TNC1 (Disease resistance protein RGA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003650 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 8.9e-133
Identity = 261/438 (59.59%), Postives = 314/438 (71.69%), Query Frame = 0

Query: 2    LYNLQTLKLGNMTSSLKNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVEFE 61
            L+NLQTLKLG++ +   NLR LV LRHLEF   Y   KMP HMG+LIHLQTLSGF   FE
Sbjct: 593  LFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPPHMGELIHLQTLSGFVAGFE 652

Query: 62   KGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWN-SREGEYEK 121
            +GCKIEELG LKNL   L L+NLE V+SK+EAIAAKLV KKNLR+L F W+     E+  
Sbjct: 653  EGCKIEELGNLKNLRGKLQLSNLEQVRSKQEAIAAKLVNKKNLRELTFEWSIDLLREFSS 712

Query: 122  CNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQLPN 181
             N+ EVLEGLQP KNL SLKI  FGGK LP G FVE L  + L  C KC+ LPMLGQLPN
Sbjct: 713  YNDFEVLEGLQPPKNLTSLKITNFGGKFLPAGTFVENLAFLCLYGCTKCERLPMLGQLPN 772

Query: 182  LKKLSIFSMDDVRSIGNEFYGIDSNQTDSFPQLKKLCIYWMKNLEQW--DEAPLVPNLFG 241
            L++L I  MD V SIGNEFYG DSN+   FP+LKK  + WM NLEQW  + A    N FG
Sbjct: 773  LQELGICFMDRVISIGNEFYGNDSNRRGYFPKLKKFDLGWMCNLEQWELEVANHESNHFG 832

Query: 242  SLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLERLPE 301
            SLQ L++  C KLTKLP+G+ECC S++ +II  CPNL LNV+ +H+L  L I+GL+ LP+
Sbjct: 833  SLQTLKLDRCGKLTKLPNGLECCKSVREVIISSCPNLTLNVEKMHSLSVLLIDGLKFLPK 892

Query: 302  GLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLIELELYNDESNIATQLPEQLEHLTALKVL 361
            GLAH  NLK + I GC ++YD+SPF++L SL +L L ND    ATQLP+QL+HLTALK+L
Sbjct: 893  GLAHLPNLKTMMITGCIEDYDYSPFLNLPSLTKLYL-NDGLGNATQLPQQLQHLTALKIL 952

Query: 362  SIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFDGCPQLLH 421
            +IE+F GI+VLPEWL  LT LETL   RCKNL++LPSR+A LCLTKL       CP LL 
Sbjct: 953  AIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSREAMLCLTKLKDFKVMACPLLLL 1012

Query: 422  G-----ERAKLSHLPEFV 432
            G     E AK  H+P ++
Sbjct: 1013 GGQADQEGAKYLHIPAYL 1029

BLAST of Lag0022677 vs. ExPASy TrEMBL
Match: A0A1S3BXM2 (disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103494527 PE=4 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 7.5e-132
Identity = 259/438 (59.13%), Postives = 313/438 (71.46%), Query Frame = 0

Query: 2    LYNLQTLKLGNMTSSLKNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVEFE 61
            L+NLQTLKLG++ +   NLR LV LRHLEF   Y   KMP HMG+LIHLQTLSGF   FE
Sbjct: 593  LFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPPHMGELIHLQTLSGFVAGFE 652

Query: 62   KGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWN-SREGEYEK 121
            +GCKIEELG LKNL   L L+NLE V+SK+EAIA KLV KKNLR+L F W+     E+  
Sbjct: 653  EGCKIEELGNLKNLRGKLQLSNLEQVRSKQEAIAVKLVDKKNLRELTFEWSIDLLREFSS 712

Query: 122  CNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQLPN 181
             N+ EVLEGLQP KNL SLKI  FGGK LP G FVE L  + L  C KC+ LPMLGQLPN
Sbjct: 713  YNDFEVLEGLQPPKNLTSLKITNFGGKFLPAGTFVENLAFLCLYGCTKCERLPMLGQLPN 772

Query: 182  LKKLSIFSMDDVRSIGNEFYGIDSNQTDSFPQLKKLCIYWMKNLEQW--DEAPLVPNLFG 241
            L++L I  +D V SIGNEFYG DSN+   FP+LKK  + WM NLEQW  + A    N FG
Sbjct: 773  LQELGICFLDRVISIGNEFYGNDSNRRGYFPKLKKFDLGWMCNLEQWELEVANHESNHFG 832

Query: 242  SLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLERLPE 301
            SLQ L++  C KLTKLP+G+ECC S++ +II  CPNL LNV+ +H+L  L I+GL+ LP+
Sbjct: 833  SLQTLKLDRCGKLTKLPNGLECCKSVREVIISNCPNLTLNVEKMHSLSVLLIDGLKFLPK 892

Query: 302  GLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLIELELYNDESNIATQLPEQLEHLTALKVL 361
            GLAH  NLK + I GC ++YD+SPF++L SL +L L ND    ATQLP+QL+HLTALK+L
Sbjct: 893  GLAHLPNLKTMMITGCIEDYDYSPFLNLPSLTKLYL-NDGLGNATQLPQQLQHLTALKIL 952

Query: 362  SIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFDGCPQLLH 421
            +IE+F GI+VLPEWL  LT LETL   RCKNL++LPSR+A LCLTKL       CP LL 
Sbjct: 953  AIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSREAMLCLTKLKDFKVMACPLLLL 1012

Query: 422  G-----ERAKLSHLPEFV 432
            G     E AK  H+P ++
Sbjct: 1013 GGQADQEGAKYLHIPAYL 1029

BLAST of Lag0022677 vs. ExPASy TrEMBL
Match: A0A0A0KXG0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016360 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 4.9e-131
Identity = 262/438 (59.82%), Postives = 311/438 (71.00%), Query Frame = 0

Query: 2   LYNLQTLKLGNMTSSLKNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAVEFE 61
           L+NLQTLKLG++ +   NLR LV LRHLEF   Y   KMPSHMG+LIHLQ LS F   FE
Sbjct: 505 LFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWFVAGFE 564

Query: 62  KGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWN-SREGEYEK 121
           +GCKIEELG LKNL+  L L+NLE V+SKEEA+AAKLV KKNLR+L F W+     E   
Sbjct: 565 EGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSS 624

Query: 122 CNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEILVEIKLRSCEKCKVLPMLGQLPN 181
            N+ EVLEGLQP KNL SLKI  FGGK LP   FVE LV + L  C KC+ LPMLGQL N
Sbjct: 625 YNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPMLGQLAN 684

Query: 182 LKKLSIFSMDDVRSIGNEFYGIDSNQTDSFPQLKKLCIYWMKNLEQW--DEAPLVPNLFG 241
           L++LSI  MD VRSIG+EFYGIDSN+   FP+LKK    WM NLEQW  + A    N FG
Sbjct: 685 LQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFG 744

Query: 242 SLQQLEIVGCEKLTKLPSGIECCNSIKYLIIRKCPNLMLNVQNLHNLYHLDINGLERLPE 301
           SLQ L++  C KLTKLP+G+ECC S+  +II  CPNL LNV+ +HNL  L I+GL+ LP+
Sbjct: 745 SLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLPK 804

Query: 302 GLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLIELELYNDESNIATQLPEQLEHLTALKVL 361
           GLA   NLK I IKGC ++YD+SPF++L SL +L L ND    ATQLP+QL+HLTALK+L
Sbjct: 805 GLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYL-NDGLGNATQLPKQLQHLTALKIL 864

Query: 362 SIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLTKLNHLFFDGCPQLLH 421
           +IE+F GI+VLPEWL  LT LETL   RCKNL++LPSR A  CLTKL       CP LL 
Sbjct: 865 AIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLLLL 924

Query: 422 G-----ERAKLSHLPEFV 432
           G     E AK  H+P ++
Sbjct: 925 GGQADQEGAKYLHIPAYL 941

BLAST of Lag0022677 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 149.1 bits (375), Expect = 1.0e-35
Identity = 136/412 (33.01%), Postives = 196/412 (47.57%), Query Frame = 0

Query: 2    LYNLQTLKLGNMTSSLKNL----RKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFA 61
            +YNLQTL L +  SSLK L      L+NLR+L+      + +MP   G+L  LQTL+ F 
Sbjct: 624  MYNLQTLLL-SYCSSLKELPTDISNLINLRYLDLIGT-KLRQMPRRFGRLKSLQTLTTFF 683

Query: 62   VEFEKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSREGE 121
            V    G +I ELG L +L   L +  L+ V    +A  A L  KK+LR++ F W +    
Sbjct: 684  VSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTGSSS 743

Query: 122  YEK-------CNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGV---FVEILVEIKLRSCE 181
             E         N  EV E L+PH++++ L I+ + G+  P+ +       +V I+LR C+
Sbjct: 744  SENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQ 803

Query: 182  KCKVLPMLGQLPNLKKLSIFSMDDVRSIGNEFYGIDSNQTDSFPQ-LKKLCIYWMKNLEQ 241
             C  LP LGQLP LK+L I  M  ++SIG +FY  D    D   Q  + L      NL  
Sbjct: 804  YCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPD 863

Query: 242  WDEAPLV----PNLFGSLQQLEIVGCEKLT-KLPSGIECCNSIKYLIIRKCPNLMLNVQN 301
            W E   V     +LF SL++L I+ C +LT  LP+ +    S+  L I KC   +L+ Q 
Sbjct: 864  WQEWLDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLP---SLISLHIYKCG--LLDFQP 923

Query: 302  LHNLYHLDINGLERLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLIELELYNDESNI 361
             H+ Y                  NL+ + IK         P  H  +L +LE+    S  
Sbjct: 924  DHHEY---------------SYRNLQTLSIKSSCDTLVKFPLNHFANLDKLEVDQCTSLY 983

Query: 362  ATQLP-EQLEHLTALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQ 393
            + +L  E L    AL+ L I D   + +LP+ L  L     +    C+ LRQ
Sbjct: 984  SLELSNEHLRGPNALRNLRINDCQNLQLLPK-LNALPQNLQVTITNCRYLRQ 1012

BLAST of Lag0022677 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 121.3 bits (303), Expect = 2.2e-27
Identity = 95/287 (33.10%), Postives = 143/287 (49.83%), Query Frame = 0

Query: 2   LYNLQTLKLGN---MTSSLKNLRKLVNLRHLEFDEPYVIEKMPSHMGKLIHLQTLSGFAV 61
           L NLQTL L N   +TS  K++ +L+NLR L+     ++E MP  + KL  LQ LS F +
Sbjct: 618 LCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVE-MPPGIKKLRSLQKLSNFVI 677

Query: 62  EFEKGCKIEELGTLKNLESTLMLTNLENVKSKEEAIAAKLVKKKNLRKLIFHWNSR---- 121
               G  + EL  L +L  TL ++ L+NV    EA  A L +K  L  LI  W  +    
Sbjct: 678 GRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLILKWTVKGSGF 737

Query: 122 -EGEYE--KCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEI---LVEIKLRSCEK 181
             G +    C+  EVL  L+PH +L++  I+++ G   P  +       +  + L SC  
Sbjct: 738 VPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSSCNL 797

Query: 182 CKVLPMLGQLPNLKKLSIFSMDDVRSIGNE-FYGIDSNQTDSFPQLKKLCIYWMKNLEQW 241
           C  LP +GQLP+LK LSI   + ++ +G + F+G ++++   F  L+ L  Y M   ++W
Sbjct: 798 CISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRWDEW 857

Query: 242 DEAPLVPNLFGSLQQLEIVGCEKL-TKLPSGIECCNSIKYLIIRKCP 274
               L   +F  LQ+L I  C  L  K P G+     +    I  CP
Sbjct: 858 ICPELEDGIFPCLQKLIIQRCPSLRKKFPEGLPSSTEV---TISDCP 900

BLAST of Lag0022677 vs. TAIR 10
Match: AT5G44510.1 (target of AVRB operation1 )

HSP 1 Score: 89.0 bits (219), Expect = 1.2e-17
Identity = 94/364 (25.82%), Postives = 156/364 (42.86%), Query Frame = 0

Query: 121  CNNLEVLEGLQPHKNLQSLK-IQAFGGKVLPNGV-FVEILVEIKLRSCEKCKVLP-MLGQ 180
            C NL+ L       NLQ L+ I       LP+ +     L+E+ L  C     LP  +G 
Sbjct: 667  CVNLKELPDFSTATNLQELRLINCLSLVELPSSIGNATNLLELDLIDCSSLVKLPSSIGN 726

Query: 181  LPNLKKLSIFSMDDVRSIGNEFYGIDSNQTDSFPQLKKLCIYWMKNLEQWDEAPLVPNLF 240
            L NLKKL +     +  + + F  + S        LK+L      NL        +P+  
Sbjct: 727  LTNLKKLFLNRCSSLVKLPSSFGNVTS--------LKEL------NLSGCSSLLEIPSSI 786

Query: 241  GSLQQLEIV---GCEKLTKLPSGIECCNSIKYL------IIRKCPNLMLNVQNLHNLYHL 300
            G++  L+ V   GC  L +LPS I    ++K L       + +CP+ MLN+  L +L   
Sbjct: 787  GNIVNLKKVYADGCSSLVQLPSSIGNNTNLKELHLLNCSSLMECPSSMLNLTRLEDLNLS 846

Query: 301  DINGLERLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLIEL-----ELYNDESNIAT 360
                L +LP  + +  NL+ + +  C      S  M L   IE       LY D  +   
Sbjct: 847  GCLSLVKLP-SIGNVINLQSLYLSDC------SSLMELPFTIENATNLDTLYLDGCSNLL 906

Query: 361  QLPEQLEHLTALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNLRQLPSRKAKLCLT 420
            +LP  + ++T L+ L +     +  LP  +EN  +L++L   +C +L +LPS   +  ++
Sbjct: 907  ELPSSIWNITNLQSLYLNGCSSLKELPSLVENAINLQSLSLMKCSSLVELPSSIWR--IS 966

Query: 421  KLNHLFFDGCPQLLHGERAKLSHLPEFVLRDPLNSVNMALKTVFGFYDPNSGILKTTFLC 468
             L++L    C  LL         +P+ ++ D  +  ++  +    F +P   I+     C
Sbjct: 967  NLSYLDVSNCSSLLELNLVSHPVVPDSLILDAGDCESLVQRLDCFFQNPK--IVLNFANC 1005

BLAST of Lag0022677 vs. TAIR 10
Match: AT4G16940.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 75.9 bits (185), Expect = 1.1e-13
Identity = 81/331 (24.47%), Postives = 140/331 (42.30%), Query Frame = 0

Query: 103 NLRKLIFH----WNSREGEYEKCNNLEVLEGLQPHKNLQSLKIQAFGGKVLPNGVFVEIL 162
           NL+ L  H    W  RE      N L  L      + L+ L+ +    K LP+    E L
Sbjct: 513 NLQFLNIHDHYWWQPRETRLRLPNGLVYLP-----RKLKWLRWENCPLKRLPSNFKAEYL 572

Query: 163 VEIKLRSCEKCKVLPMLGQLPNLKKLSIFSMDDVRSIGNEFYGIDSNQTD--------SF 222
           VE+++ +    K+      L +LKK+++ + ++++ I +     +  + D        SF
Sbjct: 573 VELRMENSALEKLWNGTQPLGSLKKMNLRNSNNLKEIPDLSLATNLEELDLCNCEVLESF 632

Query: 223 P------QLKKLCIYWMKNLEQWDEAPLVPNLFGSLQQLEIVGCEKLTKLPSGIECCNSI 282
           P       LK L +     L  + E  +   +F    ++E+  C     LP        +
Sbjct: 633 PSPLNSESLKFLNLLLCPRLRNFPEIIMQSFIFTDEIEIEVADCLWNKNLP-------GL 692

Query: 283 KYL-IIRKCPNLMLNVQNLHNLYHLDINGLERLPEGLAHQTNLKEIKIKGCRQNYDFSPF 342
            YL  +R+C       ++L NL     N LE+L EG+     LK + +  C    +    
Sbjct: 693 DYLDCLRRCNPSKFRPEHLKNLTVRGNNMLEKLWEGVQSLGKLKRVDLSECENMIEIPDL 752

Query: 343 MHLCSLIELELYNDESNIATQLPEQLEHLTALKVLSIEDFDGIDVLPEWLENLTSLETLR 402
               +L  L+L N +S +   LP  + +L  L  L++E+  G+ VLP  + NL+SL T+ 
Sbjct: 753 SKATNLEILDLSNCKSLV--MLPSTIGNLQKLYTLNMEECTGLKVLPMDI-NLSSLHTVH 812

Query: 403 FHRCKNLRQLPSRKAKLCLTKLNHLFFDGCP 415
              C +LR +P     + +  L+    +  P
Sbjct: 813 LKGCSSLRFIPQISKSIAVLNLDDTAIEEVP 828

BLAST of Lag0022677 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 72.0 bits (175), Expect = 1.5e-12
Identity = 75/324 (23.15%), Postives = 136/324 (41.98%), Query Frame = 0

Query: 103 NLRKLIFHWNSREGEYEKCNNLEVLEGLQ--PHKNLQSLKIQAFGGKVLPNGVFVEILVE 162
           NL+ L F+  S +GE      + +  GL   P K L+ L+   +  K +P+  F E LVE
Sbjct: 552 NLKLLNFYDLSFDGE----TRVHLPNGLSYLPRK-LRYLRWDGYPLKTMPSRFFPEFLVE 611

Query: 163 IKLRSCEKCKVLPMLGQLPNLKKLSIFSMDDVRSIGNEFYGIDSNQTDSFPQLKKLCIYW 222
           + + +    K+   +  L NLKK+ +             Y ++         L++L + +
Sbjct: 612 LCMSNSNLEKLWDGIQPLRNLKKMDLSRCK---------YLVEVPDLSKATNLEELNLSY 671

Query: 223 MKNLEQWDEAPLVPNLFGSLQQLEIVGCEKLTKLPSGI----------ECCNSIKYLIIR 282
            ++L   +  P + NL G L    +  C +L  +P GI            C+S+K+    
Sbjct: 672 CQSLV--EVTPSIKNLKG-LSCFYLTNCIQLKDIPIGIILKSLETVGMSGCSSLKHF--- 731

Query: 283 KCPNLMLNVQNLHNLYHLDINGLERLPEGLAHQTNLKEIKIKGCRQNYDFSPFMHLCSLI 342
             P +  N + L    +L    +E LP  ++  + L ++ +  C++              
Sbjct: 732 --PEISWNTRRL----YLSSTKIEELPSSISRLSCLVKLDMSDCQR-------------- 791

Query: 343 ELELYNDESNIATQLPEQLEHLTALKVLSIEDFDGIDVLPEWLENLTSLETLRFHRCKNL 402
                         LP  L HL +LK L+++    ++ LP+ L+NLTSLETL    C N+
Sbjct: 792 -----------LRTLPSYLGHLVSLKSLNLDGCRRLENLPDTLQNLTSLETLEVSGCLNV 824

Query: 403 RQLPSRKAKLCLTKLNHLFFDGCP 415
            + P     + + +++    +  P
Sbjct: 852 NEFPRVSTSIEVLRISETSIEEIP 824