Lag0022627 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0022627
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr7: 34617228 .. 34620416 (-)
RNA-Seq ExpressionLag0022627
SyntenyLag0022627
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTGACATGAGCAAAGCGTACAACAGGGTTGAATGGGTGTTCCTCAGGGAGATTATGGCCAAGATTGGTTTTTGTGAGGGATGGATTGCTAAGGTTATGATGTGCGTGGAATCAGTGGAATTCTCAGTCTTGATCAATGGAGTTCCTCAAGATCCGTTCATGCCGAACAGAGGGCTCAAACAAGGAGACCCCATCTCCCCCTATCTGTTCCTCCTTTGCGCTGAAGGGCTTTCGGGGCTCCTTAACAGGGAAGAATCCTCTCTCAAATTTAGAGGCATTAGAATAAACAAAATTTGCCATTCCATTTCTCATTTATTCTTTGTTGACGATAGCTTGGTTTTTTGTAGGGCTGAGGAGAATGATTGCAGTACTATAAAGGGTATTCTAGAGACGTACGAGAAGGCATCAGGTCAAACCATAAATCTCAACAAATCTGTCTTCATGGCCAGTAGAAACATAAAACATTAGAAGTTGAAGAAGTTGGAAGAGTTGTTGGGTATCAGACAAGCTCAAGAGATAGGCCATTATCTTGGCATGTCATCTCAGAATAAAAGAAGAAAAACAGTCCTTTTTAGTAAGCTGAAGGAGAGAGTGGAAAAGGCCCTTCAAGGCTGGAAGGGGAGCCTTTTTTCTATGGGGAAAAGAGATCCTTATAAAGGCAGTGGTTCAAGCAATCCCCACGTATACGATGTCATGTTTCAAGCTCCCAAAAAACCTTTGTGACGACATCAACAAAGCTTGCGCTCGGTTTTGGTGGGGGGCCTCAAAAAACAAGAGGAAAACACATTGGATGAGCTGGAGGAAGCTTTGTAAAGGAAAAGGGAATCGGTGGCATGGGCTTTAGAGACATACATCTTTTCAACCAAGCTATGCTAGCTAAACAGAGTTGGAGAATAGTAAGGAACCCTCAAAGTCTACTAGCAAAGGTCCTAAGGGGTAGATACTTCAAGGATAAAGACTTCTTTAAAGCCCCCCTAGGGAACAACTCATCCCTGACTTGGTGAAACATTGTGTGGGGGAGAGAATTGTTTGTTAAGGGATATCGTTGGAAAGTTGGAAATGACAATCACATTAAGATAGGCACCAACCCCTGGCTGAAAAGAAAAGGGGACATGATGGTAAGAAGCTCCCCAGATATTCTCTGAGATGATAGAGTCAGTAGCTTAATAAGGGAAGAGGGTGACTGGAATGAGAGGCTCATTAAGGAGCTTTTTTCAGATCAGGATGCCGAGGAGATTCTAAAGATCCCGCTGGGAAGCAAAAATGCTATGGACGAAATCATATGGGCTAAGCACCCTAAAGGGTCTTTCTCTGTGAAAACAGCCTACCATCTGGCGGTTAACCAAGAAGAGCATCGAAAAGCTAGCTGCTCGGATAATTCCAAGATCCAAGCTATATGGAAGAACTTTTGGAAGATAAAAGCAATCCCCAGAGCCAAAATCTGTGTGTGGAAAATGATAAACAACAGTATCCCATCCAAAATGAACATTATATCAAAAGGCATTGAAACTAACTCATTGTGCCCTTTGTGCAGGAGCAAATAGGAATCAAACACGCATTTGATATGGGAGTGTAAGTTCTCTAAGAAAATCTGGTTGAAGTTTCTCCCTAATGTTGGTTCCCTTTTTTACATATGCAGGCCTGCGTGGGATGCTCTATTGATTTGGGAAGGTCTAGTGGAAAGACTCGAGGAGGAAGAGATAAGCATCGCCATCCTAATTCTATGGAATATTTGGACAACTCGGAACAAGGTGATCAACAATGGCTACAAAGCAGATCAAAATCAAATCAGTAAAATTATTGAAGCCAACATATCCGAGCATTTCAAGGTTAGAGATACTAACCTGAGTGCTTCTAGATCGGAGAGTCATCATAGTCAATATTTGTGGTCTCCTCCTCCCCCACCTTTTCTGAAGCTGAATTCCGATGCCTCTTGGAGCAGTTCAGAAGAAATTGGGGGCTTAGGTTGGACGGTTCGTGACTCCTCAGGGTCTCTGGTATTCGCCGGCAACATGAAGACAAAGAAGAATTGGGAAACGTTGGAGTTGGAAGCTCTAGCTATGAAGGAAGGGTTGTCTTCTTTCTTGCAAAATAGCGCATGCAAGGATCGCCCTCTTGTGGTTGAATCGGACTCGAGCACTCTGATTAAGTTGATCCAAAATCAAGAGTCTGATCTCTCTGAGGTAAGAAGCATTGTGGAAGAGATTGGGCTTCTGGCAAGGAGCGCAATAGGAATCTCCTTCGTTTGGTGTTCGAGAAGCTGCAATCGAGCAACCCACACCCTTGCTAAGGATGCGAGATTGGCAGGAACTATAGAGTTTCCTTTGATTTTTGTTGATGGTACCTTTGTATTCCCTGTTGGGAGGAGGTGGTTTTGTTGGGCAATCTGTTTTGCCAAAGCTAGCTCTATTATTAATGAGAAAAAAAAGTTTATTGCTGGTTGTTAGTTGCGTTTAATGAATTATGTTTATTTCAAAAAAAAAATTGGATTTTTTTTTTTTTTTGAGAAAGTTAATTTGGATTTGAATAATGGAAAAATAATGGAATTAACGTCCACAAACAGAGCATGTTACTCCGACATGTAGCAACCTGGCTTTTCCAACAACAGACAAGTGGCGTTGGGCCATCACGTGATCATCACGCGCACTTTCAAACAATTCCAATTTACCAATGTACCCTCGTCTCATGCTAGCCGTTGCAACCCTTTCTTTCTCGCTGTAGTAATACCCAAGGGCAAACTCGTAAGTTCGTATCTCCTTTTCCCTCTCTTCTCTCTCTCCCAACTGTTGTTTTGCTCCTTCCGTGGTCAATCAAAATCCACCAAATCGAAGCTTTTCCTCACTAACACTCTCACTGAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCGTTTCGCCGAGATTCTCTCTCCTAGGGTTTAGGTTTTGGTTGCAGTTTCCATGGCTTCTTCGTCCTCTGATCCTGGCTTCAAACACGAAGCCGGTGCCTGTAGCGCTGCTGCTGCTGGAGGAGGAACGGCGGAGTCTTCCGAGGTTGTAATGGCGAATGATCAGCTTTTACTGTACAGAGGATTGAAGAAAGCGAAGAAAGAGAGAGGTTGCACTGCCAAAGAGCGCATCAGTAAAATGCCTCCATGTGCTGCTGGAAAAAGAAGCTCCATATATCGCGGAGTCACTAGGTAA

mRNA sequence

ATGAAGCTTGACATGAGCAAAGCGTACAACAGGGTTGAATGGGTGTTCCTCAGGGAGATTATGGCCAAGATTGGTTTTTGTGAGGGATGGATTGCTAAGGTTATGATGTGCGTGGAATCAGTGGAATTCTCAGTCTTGATCAATGGAGTTCCTCAAGATCCGTTCATGCCGAACAGAGGGCTCAAACAAGGAGACCCCATCTCCCCCTATCTGTTCCTCCTTTGCGCTGAAGGGCTTTCGGGGCTCCTTAACAGGGAAGAATCCTCTCTCAAATTTAGAGGCATTAGAATAAACAAAATTTGCCATTCCATTTCTCATTTATTCTTTGTTGACGATAGCTTGGTTTTTTGTAGGGCTGAGGAGAATGATTGCAGTACTATAAAGGGTATTCTAGAGACGTACGAGAAGGCATCAGATCAGGATGCCGAGGAGATTCTAAAGATCCCGCTGGGAAGCAAAAATGCTATGGACGAAATCATATGGGCTAAGCACCCTAAAGGGTCTTTCTCTGTGAAAACAGCCTACCATCTGGCGGTTAACCAAGAAGAGCATCGAAAAGCTAGCTGCTCGGATAATTCCAAGATCCAAGCTATATGGAAGAACTTTTGGAAGATAAAAGCAATCCCCAGAGCCAAAATCTGTGAGCAAATAGGAATCAAACACGCATTTGATATGGGAGTGCCTGCGTGGGATGCTCTATTGATTTGGGAAGGTCTAGTGGAAAGACTCGAGGAGGAAGAGATAAGCATCGCCATCCTAATTCTATGGAATATTTGGACAACTCGGAACAAGGTGATCAACAATGGCTACAAAGCAGATCAAAATCAAATCAGTAAAATTATTGAAGCCAACATATCCGAGCATTTCAAGGTTAGAGATACTAACCTGAGTGCTTCTAGATCGGAGAGTCATCATAGTCAATATTTGTGGTCTCCTCCTCCCCCACCTTTTCTGAAGCTGAATTCCGATGCCTCTTGGAGCAGTTCAGAAGAAATTGGGGGCTTAGGTTGGACGGTTCGTGACTCCTCAGGGTCTCTGGTATTCGCCGGCAACATGAAGACAAAGAAGAATTGGGAAACGTTGGAGTTGGAAGCTCTAGCTATGAAGGAAGGGTTGTCTTCTTTCTTGCAAAATAGCGCATGCAAGGATCGCCCTCTTGTGGTTGAATCGGACTCGAGCACTCTGATTAAGTTGATCCAAAATCAAGAGTCTGATCTCTCTGAGGTAAGAAGCATTGTGGAAGAGATTGGGCTTCTGGCAAGGAGCGCAATAGGAATCTCCTTCGTTTGGTGTTCGAGAAGCTGCAATCGAGCAACCCACACCCTTGCTAAGGATGCGAGATTGGCAGGAACTATAGAGTTTCCTTTGATTTTTGTTGATGTTTCCATGGCTTCTTCGTCCTCTGATCCTGGCTTCAAACACGAAGCCGGTGCCTGTAGCGCTGCTGCTGCTGGAGGAGGAACGGCGGAGTCTTCCGAGGTTGTAATGGCGAATGATCAGCTTTTACTGTACAGAGGATTGAAGAAAGCGAAGAAAGAGAGAGGTTGCACTGCCAAAGAGCGCATCAGTAAAATGCCTCCATGTGCTGCTGGAAAAAGAAGCTCCATATATCGCGGAGTCACTAGGTAA

Coding sequence (CDS)

ATGAAGCTTGACATGAGCAAAGCGTACAACAGGGTTGAATGGGTGTTCCTCAGGGAGATTATGGCCAAGATTGGTTTTTGTGAGGGATGGATTGCTAAGGTTATGATGTGCGTGGAATCAGTGGAATTCTCAGTCTTGATCAATGGAGTTCCTCAAGATCCGTTCATGCCGAACAGAGGGCTCAAACAAGGAGACCCCATCTCCCCCTATCTGTTCCTCCTTTGCGCTGAAGGGCTTTCGGGGCTCCTTAACAGGGAAGAATCCTCTCTCAAATTTAGAGGCATTAGAATAAACAAAATTTGCCATTCCATTTCTCATTTATTCTTTGTTGACGATAGCTTGGTTTTTTGTAGGGCTGAGGAGAATGATTGCAGTACTATAAAGGGTATTCTAGAGACGTACGAGAAGGCATCAGATCAGGATGCCGAGGAGATTCTAAAGATCCCGCTGGGAAGCAAAAATGCTATGGACGAAATCATATGGGCTAAGCACCCTAAAGGGTCTTTCTCTGTGAAAACAGCCTACCATCTGGCGGTTAACCAAGAAGAGCATCGAAAAGCTAGCTGCTCGGATAATTCCAAGATCCAAGCTATATGGAAGAACTTTTGGAAGATAAAAGCAATCCCCAGAGCCAAAATCTGTGAGCAAATAGGAATCAAACACGCATTTGATATGGGAGTGCCTGCGTGGGATGCTCTATTGATTTGGGAAGGTCTAGTGGAAAGACTCGAGGAGGAAGAGATAAGCATCGCCATCCTAATTCTATGGAATATTTGGACAACTCGGAACAAGGTGATCAACAATGGCTACAAAGCAGATCAAAATCAAATCAGTAAAATTATTGAAGCCAACATATCCGAGCATTTCAAGGTTAGAGATACTAACCTGAGTGCTTCTAGATCGGAGAGTCATCATAGTCAATATTTGTGGTCTCCTCCTCCCCCACCTTTTCTGAAGCTGAATTCCGATGCCTCTTGGAGCAGTTCAGAAGAAATTGGGGGCTTAGGTTGGACGGTTCGTGACTCCTCAGGGTCTCTGGTATTCGCCGGCAACATGAAGACAAAGAAGAATTGGGAAACGTTGGAGTTGGAAGCTCTAGCTATGAAGGAAGGGTTGTCTTCTTTCTTGCAAAATAGCGCATGCAAGGATCGCCCTCTTGTGGTTGAATCGGACTCGAGCACTCTGATTAAGTTGATCCAAAATCAAGAGTCTGATCTCTCTGAGGTAAGAAGCATTGTGGAAGAGATTGGGCTTCTGGCAAGGAGCGCAATAGGAATCTCCTTCGTTTGGTGTTCGAGAAGCTGCAATCGAGCAACCCACACCCTTGCTAAGGATGCGAGATTGGCAGGAACTATAGAGTTTCCTTTGATTTTTGTTGATGTTTCCATGGCTTCTTCGTCCTCTGATCCTGGCTTCAAACACGAAGCCGGTGCCTGTAGCGCTGCTGCTGCTGGAGGAGGAACGGCGGAGTCTTCCGAGGTTGTAATGGCGAATGATCAGCTTTTACTGTACAGAGGATTGAAGAAAGCGAAGAAAGAGAGAGGTTGCACTGCCAAAGAGCGCATCAGTAAAATGCCTCCATGTGCTGCTGGAAAAAGAAGCTCCATATATCGCGGAGTCACTAGGTAA

Protein sequence

MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRGLKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAEENDCSTIKGILETYEKASDQDAEEILKIPLGSKNAMDEIIWAKHPKGSFSVKTAYHLAVNQEEHRKASCSDNSKIQAIWKNFWKIKAIPRAKICEQIGIKHAFDMGVPAWDALLIWEGLVERLEEEEISIAILILWNIWTTRNKVINNGYKADQNQISKIIEANISEHFKVRDTNLSASRSESHHSQYLWSPPPPPFLKLNSDASWSSSEEIGGLGWTVRDSSGSLVFAGNMKTKKNWETLELEALAMKEGLSSFLQNSACKDRPLVVESDSSTLIKLIQNQESDLSEVRSIVEEIGLLARSAIGISFVWCSRSCNRATHTLAKDARLAGTIEFPLIFVDVSMASSSSDPGFKHEAGACSAAAAGGGTAESSEVVMANDQLLLYRGLKKAKKERGCTAKERISKMPPCAAGKRSSIYRGVTR
Homology
BLAST of Lag0022627 vs. NCBI nr
Match: XP_030477911.1 (uncharacterized protein LOC115694948 [Cannabis sativa])

HSP 1 Score: 199.1 bits (505), Expect = 9.6e-47
Identity = 158/635 (24.88%), Postives = 256/635 (40.31%), Query Frame = 0

Query: 1   MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
           +KLDMSKA++RVEW +L  +M K+GF   W+  +M C+ +  FS  +NG       P+RG
Sbjct: 93  LKLDMSKAFDRVEWQYLEAVMLKMGFASWWVTLIMNCITTSSFSFSLNGDVVGHVQPSRG 152

Query: 61  LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
           L+QGDP+SPYLFL+C+EGLS +L  EES    RG+ I +   SISHL F DDSL+FC+A 
Sbjct: 153 LRQGDPLSPYLFLICSEGLSRILQFEESQRNLRGLSITRNAPSISHLLFADDSLLFCQAS 212

Query: 121 ENDCSTIKGILETYEKASDQ---------------------------------------- 180
           +     IK +L TY +AS Q                                        
Sbjct: 213 DQSALAIKKVLHTYHQASGQVLNNDKSVMSFSPNTSVATQNFFAQTLQMPITDCHEKYLG 272

Query: 181 -------DAEEILK-----------------IPLGSK----------------------- 240
                  D +E+                     +G K                       
Sbjct: 273 LPSYSGRDKQELFSSIKEKVWKLLHSWNEKIFSIGGKEVLLKAVVQSIPTYAMSCFKLTK 332

Query: 241 -------------------NAMDEIIWAKHPKGSFSVKTAYHLAVNQEEHRKASCSDNSK 300
                              NA D +IW     G ++VK+ +HLA   E+   +S SD ++
Sbjct: 333 KFCNQLESMMANFWWGTNQNADDRLIWHHSSNGIYNVKSGFHLATTLEDQLISSTSDVNR 392

Query: 301 IQAIWKNFWKIKAIPRAKIC-----------------EQIGIKHAFDMGVPAWDAL---- 360
               WK FW +K  P+ +I                   +I    A  +   +W+++    
Sbjct: 393 --DWWKYFWSLKLPPKIRIFAWKVFQEILPTAAGLFKRKIIDSAACSLCTSSWESIGHAL 452

Query: 361 -------LIWEGLVERLE---------------------EEEISIAILILWNIWTTRNKV 420
                   +W+    R++                     +E+  + + +LW +WT RN+V
Sbjct: 453 FGCQHAKAVWKKSKFRIDFNKAQSMFKGDYLHYLSGVYNQEDFELFLCLLWGVWTNRNRV 512

Query: 421 INNGYKADQNQISKIIEANISEHFKVRDTNLSASRSESHHS-----------QYLWSPPP 463
            + G   D + I        ++  K +      + +  +HS           Q  WSPP 
Sbjct: 513 FHGGKSRDTSSIIAYTTGFYNDFSKAKTAPKPVAVAGPNHSHQMASSPVQSNQIPWSPPS 572

BLAST of Lag0022627 vs. NCBI nr
Match: GAU36089.1 (hypothetical protein TSUD_277000 [Trifolium subterraneum])

HSP 1 Score: 191.4 bits (485), Expect = 2.0e-44
Identity = 133/456 (29.17%), Postives = 223/456 (48.90%), Query Frame = 0

Query: 1   MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
           +KLD+SKA++RV+W +L+ +M K+GF + W+  +M CV ++ + VL+N     P  P  G
Sbjct: 489 LKLDVSKAFDRVKWSYLQAVMQKMGFSDTWVKWIMQCVTTMNYHVLLNNDRVGPITPLCG 548

Query: 61  LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
           L+QGDP+SPYL+++C EGL+  ++  ES     G+RI +   SISHL F DDS +FC+A 
Sbjct: 549 LRQGDPLSPYLYIICYEGLTSYIHHYESHGMLHGVRICRGSPSISHLLFADDSFLFCKAS 608

Query: 121 ENDCSTIKGILETYEKASDQDAEEILKIPLGSKNA-------MDEIIWAKHPKGSFSVKT 180
             + + +K +L+TYE+AS Q           S+N        ++ I+      G      
Sbjct: 609 VLEVTNLKHVLDTYEEASGQAMNYQKSAIAYSRNTEANCRSFINNILGVVESMGH----- 668

Query: 181 AYHLAVNQEEHRKASCSDNSKIQAIWKNF--WKIKAIPRA---KICEQIGIKHAFDMGVP 240
             +L +     R      +     IWK    W  +++ RA   ++     ++H F +   
Sbjct: 669 GKYLGLPSMVGRDKKSIFSFIKDRIWKKIQNWNSRSLSRAGKEEVNLWQSLEHQF-LQSG 728

Query: 241 AWDALLIWEGLVERLEEEEISIAILILWNIWTTRNKVINNGYKADQNQISKIIEANISEH 300
           ++ +++    ++  +EE + S+   +LW+IW  RN+ I    +A+    S  +  ++   
Sbjct: 729 SFSSIIF--SIISSMEETKQSLFAAVLWSIWRARNECIWENKQANP-VASCRLAFDLIRD 788

Query: 301 FKVRDTNLSASRSESHHSQYLWSPPPPPFLKLNSDASWSSSEEIGGLGWTVRDSSGSLVF 360
           F      L+A    +H   + W  PP  +LK N D +   +E   G+G   RDSSGS V 
Sbjct: 789 FNWCHNMLNADHMPTH--VHTWEKPPVNWLKCNVDGAIFMTEGKFGIGICFRDSSGSFVQ 848

Query: 361 AGNMKTKKNWETLELEALAMKEGLSSFLQNSACKDRPLVVESDSSTLIKLIQNQESDLSE 420
           A  M        +E EA AMK  L+  L N       ++ ESD   ++  ++N     +E
Sbjct: 849 ARTMTFPFEVTDVECEATAMKHALALALSNGF---ERVLFESDCQQVVNALRNDYLYANE 908

Query: 421 VRSIVEEIGLLARSAIGISFVWCSRSCNRATHTLAK 445
           + +++     L  S    +  +  R  NR  H LA+
Sbjct: 909 LGTLLSTCSSLLISNANYNVAYVRRQANRVAHNLAR 930

BLAST of Lag0022627 vs. NCBI nr
Match: XP_022157437.1 (uncharacterized protein LOC111024135 [Momordica charantia])

HSP 1 Score: 188.0 bits (476), Expect = 2.2e-43
Identity = 90/161 (55.90%), Postives = 117/161 (72.67%), Query Frame = 0

Query: 1   MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
           MKLDMSKAY+RVEW +LR IM K+GF   W+  +M CVESV F+VLINGVP D F PNRG
Sbjct: 393 MKLDMSKAYDRVEWQYLRGIMVKMGFANRWVDLIMNCVESVRFAVLINGVPGDEFTPNRG 452

Query: 61  LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
           L+QGDP+SPYLF++CAEGLS L+N EE       ++IN+ C  ISHLF+ DD L+F +A 
Sbjct: 453 LRQGDPLSPYLFIMCAEGLSALINDEEKKGNITRLKINRRCPPISHLFYADDCLLFFKAS 512

Query: 121 ENDCSTIKGILETYEKASDQDAEEILK-IPLGSKNAMDEII 161
             +C +IKGILE+YEKAS      + K + + SKN  ++++
Sbjct: 513 TGNCRSIKGILESYEKASSGQCINLDKSMFIVSKNTKEQMV 553

BLAST of Lag0022627 vs. NCBI nr
Match: BAH93889.1 (Os07g0417700 [Oryza sativa Japonica Group])

HSP 1 Score: 187.6 bits (475), Expect = 2.9e-43
Identity = 149/496 (30.04%), Postives = 225/496 (45.36%), Query Frame = 0

Query: 2   KLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRGL 61
           KLD+SKAYNRV+W FL + M K+GF   W++ +M C+ SV FSV  NG   D F P+RGL
Sbjct: 266 KLDLSKAYNRVDWTFLEQSMYKLGFSHRWVSWIMECITSVRFSVKFNGTLLDTFAPSRGL 325

Query: 62  KQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAEE 121
           +QGDP+SP+L L  A+GLS LL  + S      + I +    ISHL F DD+L+F +++ 
Sbjct: 326 RQGDPLSPFLLLFVADGLSLLLEYKVSQGAITPVHICRRALGISHL-FADDTLLFFKSDN 385

Query: 122 NDCSTIKGILETYEKASDQDAEEI-LKIPLGSKNAMDEIIWAKHP----KGSFSVKTAYH 181
                IK  L TY  A+ Q        I  GS +        KH       SF  K   +
Sbjct: 386 QQAKVIKETLSTYAAATGQLINPAKCSILFGSSSTSAVCDAIKHTLQIVNSSFEDK---Y 445

Query: 182 LAVNQEEHRKASCSDNSKIQAIWKNFWKIKAIPRAKICEQIGIKHAFD-MGVPAWDALLI 241
           L     E R       +  + IWK          +   +++ IK     + V       +
Sbjct: 446 LGFPTPEGRMNKGKFQTLQEKIWKRIISWGENLLSSGGKEVLIKSVIQAIPVYVMGMFKL 505

Query: 242 WEGLVERLEE------------EEISIAILILWNIWTTRNKVINNGYKADQNQISK-IIE 301
            E + E L              EE  ++++I+W  W  RN+ + +G      ++SK  +E
Sbjct: 506 PESVCEDLNRLTRNFWWGADNGEERDVSLMIMWRNWFVRNE-LTHGKSPPPVEVSKRFLE 565

Query: 302 ANISEHFKVRD-----------------TNLSASRSESHHSQYLWSPPPPPFLKLNSDAS 361
           + +   F +R                   +   S+ +   S  LWS P   ++K+N D S
Sbjct: 566 SYLQSLFMIRQHPTAELTKGKQVVYMYPRDREKSKRQRVASDLLWSCPETGWMKVNVDGS 625

Query: 362 WSSSEEIGGLGWTVRDSSGSLVFAGNMKTKKNWETLELEALAMKEGLSSFLQNSACKDRP 421
           + + +E GG+G  +R+S G ++FA           LE E  A +EG+    Q + C   P
Sbjct: 626 FDAQQESGGIGVVMRNSEGRVIFAAFKSLNGCKSALEAELEACREGIVLASQWTMC---P 685

Query: 422 LVVESDSSTLIKLIQNQESDLSEVRSIVEEIGLLARSAIGISFVWCSRSCNRATHTLAKD 461
             +ESD    +++IQ +E + SE+  ++ EI   A     I      RS N  +H LA  
Sbjct: 686 FYIESDCQVALQMIQGKEEERSELAYLIREIVSAAFGNREIRLRKIHRSQNSVSHYLANK 745

BLAST of Lag0022627 vs. NCBI nr
Match: XP_030502823.1 (uncharacterized protein LOC115717993 [Cannabis sativa])

HSP 1 Score: 185.7 bits (470), Expect = 1.1e-42
Identity = 168/652 (25.77%), Postives = 253/652 (38.80%), Query Frame = 0

Query: 1    MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
            +KLDMSKA +RVEW F+ E+M K+GF   WI  +M C+ +  FS L+NG P    +P+RG
Sbjct: 554  LKLDMSKALDRVEWRFIEEVMTKMGFACEWITLIMNCLRTNSFSFLLNGEPLGSLIPSRG 613

Query: 61   LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
            L+QG P+SPYLFL+C+EGLS LL  EE+S   +G ++ +   SISHLFF DDSL+FC+A 
Sbjct: 614  LRQGCPLSPYLFLICSEGLSRLLQHEETSGNLKGFKLTRHAPSISHLFFADDSLLFCQAN 673

Query: 121  ENDCSTIKGILETYEKASDQ---------------------------------------- 180
            E+ C  +K  LE Y++AS Q                                        
Sbjct: 674  ESSCLALKRSLEIYKRASGQLESMMANFWWGLNENGNKIHWKSWNLLCKRKDSGAMGFRS 733

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 734  FVLKSRYYPNNSILEANPGHSPSLTWQGIHWGKGLLVAGLRWKIGEGCRVNISRDPWMPG 793

Query: 241  ---------------------------------------DAEEILKIPLGSKNAMDEIIW 300
                                                   D + IL IPL      D +IW
Sbjct: 794  HSSFLPYHYSSPPEAVVSNLITDERLWDLDLLNEWFSPPDVDRILTIPLSFFRKDDVLIW 853

Query: 301  AKHPKGSFSVKTAYHLAVNQEEHRKASCSDNSKIQAIWKNFWKIK--------------- 360
              +  G ++V+T YH A + E+   +S S  S   A WK  W +K               
Sbjct: 854  NPNSSGIYTVQTGYHFAASLEDSDNSSSS--SSTAAWWKFLWSLKLPEKIKIFVWRTFNE 913

Query: 361  AIPRA--------------KICEQI--GIKHAF---DMGVPAW----------------- 420
            A+P A               IC Q    I HA          W                 
Sbjct: 914  ALPVATALVKQKVISDSTCSICHQAWESIGHALFRCKYARAVWRHSNFLLDWNNSSTMCK 973

Query: 421  -DALLIWEGLVERLEEEEISIAILILWNIWTTRNKVINNGYKADQNQISKIIEANISEHF 447
             D L+    +  ++E E+I   + I+W+IWT RN+V+ +G +A   ++      N  ++F
Sbjct: 974  GDYLVHLSSIYTKVEMEQI---LCIMWSIWTERNRVV-HGNRAKSAKLLSSFALNYWQNF 1033

BLAST of Lag0022627 vs. ExPASy Swiss-Prot
Match: Q8GWK2 (AP2-like ethylene-responsive transcription factor At2g41710 OS=Arabidopsis thaliana OX=3702 GN=At2g41710 PE=1 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 1.0e-22
Identity = 64/81 (79.01%), Postives = 66/81 (81.48%), Query Frame = 0

Query: 463 MAS-SSSDPGFKHEAGACSAAAAGGGTAESSEVVMANDQLLLYRGLKKAKKERGCTAKER 522
           MAS SSSD G K EAG CS    GGG  ESSE V A+DQ+LLYRG KKAKKERGCTAKER
Sbjct: 1   MASVSSSDQGPKTEAG-CS----GGGGGESSETVAASDQMLLYRGFKKAKKERGCTAKER 60

Query: 523 ISKMPPCAAGKRSSIYRGVTR 543
           ISKMPPC AGKRSSIYRGVTR
Sbjct: 61  ISKMPPCTAGKRSSIYRGVTR 76

BLAST of Lag0022627 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-13
Identity = 35/68 (51.47%), Postives = 46/68 (67.65%), Query Frame = 0

Query: 46  LINGVPQDPFMPNRGLKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSIS 105
           +ING PQ    P+RGL+QGDP+SPYLF+LC E LSGL  R +   +  GIR++     I+
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 106 HLFFVDDS 114
           HL F DD+
Sbjct: 73  HLLFADDT 80

BLAST of Lag0022627 vs. ExPASy Swiss-Prot
Match: X5HYT8 (AP2-like ethylene-responsive transcription factor SMOS1 OS=Oryza sativa subsp. japonica OX=39947 GN=SMOS1 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.1e-08
Identity = 46/95 (48.42%), Postives = 53/95 (55.79%), Query Frame = 0

Query: 463 MASSSSDPGFKHEAGACSAAAAGGGTAE-------------SSEVVMANDQLLL--YRGL 522
           MAS     G + +  A +AAA GG  AE               E V A  + +    R  
Sbjct: 1   MASPGPAAGMQQKLEAAAAAAGGGDGAEWGRGMQKMEAVGAGGEGVGAGAEQVAPPPRRP 60

Query: 523 KKAKKERGCTAKERISKMPPCAAGKRSSIYRGVTR 543
             A+KER CTAKERIS+MPPCAAGKRSSIYRGVTR
Sbjct: 61  VAARKERVCTAKERISRMPPCAAGKRSSIYRGVTR 95

BLAST of Lag0022627 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 1.1e-05
Identity = 30/134 (22.39%), Postives = 67/134 (50.00%), Query Frame = 0

Query: 1   MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
           + LD  KA+++++  F+ +++ + G    ++  +         ++ +NG   +      G
Sbjct: 604 ISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSG 663

Query: 61  LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
            +QG P+SPYLF +  E L+  + +++   + +GI+I K    IS L   DD +V+    
Sbjct: 664 TRQGCPLSPYLFNIVLEVLARAIRQQK---EIKGIQIGKEEVKISLL--ADDMIVYISDP 723

Query: 121 ENDCSTIKGILETY 135
           +N    +  ++ ++
Sbjct: 724 KNSTRELLNLINSF 732

BLAST of Lag0022627 vs. ExPASy Swiss-Prot
Match: Q05118 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 7.2e-05
Identity = 32/115 (27.83%), Postives = 53/115 (46.09%), Query Frame = 0

Query: 1   MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
           + LD+ KA++ V    +   M   G  +G    +M  +     ++++ G   +      G
Sbjct: 26  VSLDIRKAFDTVSHPAILRAMRAFGIDDGMQDFIMSTITDAYTNIVVGGRTTNKIYIRNG 85

Query: 61  LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLV 116
           +KQGDP+SP LF +  + L   LN E+      G  +   C  I+ L F DD L+
Sbjct: 86  VKQGDPLSPVLFNIVLDELVTRLNDEQP-----GASMTPAC-KIASLAFADDLLL 134

BLAST of Lag0022627 vs. ExPASy TrEMBL
Match: A0A803NML1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 9.0e-51
Identity = 166/594 (27.95%), Postives = 253/594 (42.59%), Query Frame = 0

Query: 1    MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
            +KLDMSKA++RVEW +L  IM K+GF   WI+ +M C+ +  FS  +NG       P+RG
Sbjct: 1030 LKLDMSKAFDRVEWRYLEAIMEKMGFDHLWISLIMRCISTSSFSFSLNGDIVGHVTPHRG 1089

Query: 61   LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
            L+QGDP+SPYLFL+C+EGLS  L  +E S   +G+ + +   S+SHL F DDSL+FC++ 
Sbjct: 1090 LRQGDPLSPYLFLICSEGLSRFLQAQEQSGMLKGLCLTRNAPSVSHLLFADDSLLFCQST 1149

Query: 121  ENDCSTIKGILETYEKASDQ---------------------------------------- 180
            E    +IK  L+TY +AS Q                                        
Sbjct: 1150 EQSAISIKRSLDTYHRASGQELLLKGLRFKIGDGIHVDNRKDPWIPSHNDFKPVSYLGSD 1209

Query: 181  --------------------------DAEEILKIPLGSKNAMDEIIWAKHPKGSFSVKTA 240
                                      D + IL IPL      D ++W   P G +SVKT 
Sbjct: 1210 SLPVSHFITDNRVWNVPLLTSYFQQIDIDRILSIPLAYFAGTDRLVWHHSPNGIYSVKTG 1269

Query: 241  YHLAVNQEEHRKASCSDNSKIQAIWKNFWKIKAIPRAKI--------------------- 300
            +HLA   E+   +S S  +K    WK FW +K  P+ +I                     
Sbjct: 1270 FHLATTLEDQNSSSTS--NKQSEWWKFFWNLKLPPKIRIFAWKVFQNILPTAVALFKRKV 1329

Query: 301  ------------CEQIG-----IKHAFDM------GVPAWDALLIWEG-----LVERLEE 360
                         E IG      KHA D+       +    A  ++ G     L    ++
Sbjct: 1330 LDSGECSLCTSNWESIGHALFSCKHAKDIWKISRFRIDFSQAHNMFNGDYLYHLSTIYQQ 1389

Query: 361  EEISIAILILWNIWTTRNKVINNGYKADQNQISKIIEANISEHFKVRDTNLSASRSESHH 420
             +    + +LW IWT RNKV++ G       I +       +  K +    S + +  HH
Sbjct: 1390 HDFETLLCVLWGIWTDRNKVVHGGQPRHPTAIVQYASKFYEDFNKAKLQIPSVAATSRHH 1449

Query: 421  SQ----------YLWSPPPPPFLKLNSDASWSSSEEIGGLGWTVRDSSGSLVFAGNMKTK 462
            S             W PP     KLN DA+ +  ++  G+G  +RD  G+++ A +   +
Sbjct: 1450 SSPSTSATDQHVQRWRPPVRNGYKLNVDAATNIEQKKMGIGAILRDHQGTVLAALSKAVQ 1509

BLAST of Lag0022627 vs. ExPASy TrEMBL
Match: A0A803PM52 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 9.3e-48
Identity = 168/611 (27.50%), Postives = 253/611 (41.41%), Query Frame = 0

Query: 1    MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
            +KLDMSKA +RVEW F+ E+M K+GF   WI  +M C+ +  FS L+NG P    +P+RG
Sbjct: 906  LKLDMSKALDRVEWRFIEEVMTKMGFACEWITLIMNCLRTNSFSFLLNGEPLGSLIPSRG 965

Query: 61   LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
            L+QG P+SPYLFL+C+EGLS LL  EE+S   +G ++ +   SISHLFF DDSL+FC+A 
Sbjct: 966  LRQGCPLSPYLFLICSEGLSRLLQHEETSGNLKGFKLTRHAPSISHLFFADDSLLFCQAN 1025

Query: 121  ENDCSTIKGILETYEKASDQ---------------------------------------- 180
            E+ C  +K  LE Y++AS Q                                        
Sbjct: 1026 ESSCLALKRSLEIYKRASGQVLKSRYYPNNSILEANPGHSPSLTWQGIHWGKGLLVAGLR 1085

Query: 181  ----------------------------------------------------------DA 240
                                                                      D 
Sbjct: 1086 WKIGEGCRVNISRDPWMPGHSSFLPYHYSSPPEAVVSNLITDERLWDLDLLNEWFSPPDV 1145

Query: 241  EEILKIPLGSKNAMDEIIWAKHPKGSFSVKTAYHLAVNQEEHRKASCSDNSKIQAIWKNF 300
            + IL IPL      D +IW  +  G ++V+T YH A + E+   +S S  S   A WK  
Sbjct: 1146 DRILTIPLSFFRKDDVLIWNPNSSGIYTVQTGYHFAASLEDSDNSSSS--SSTAAWWKFL 1205

Query: 301  WKIK---------------AIPRA--------------KICEQI--GIKHAF---DMGVP 360
            W +K               A+P A               IC Q    I HA         
Sbjct: 1206 WSLKLPEKIKIFVWRTFNEALPVATALVKQKVISDSTCSICHQAWESIGHALFRCKYARA 1265

Query: 361  AW------------------DALLIWEGLVERLEEEEISIAILILWNIWTTRNKVINNGY 420
             W                  D L+    +  ++E E+I   + I+W+IWT RN+V+ +G 
Sbjct: 1266 VWRHSNFLLDWNNSSTMCKGDYLVHLSSIYTKVEMEQI---LCIMWSIWTERNRVV-HGN 1325

Query: 421  KADQNQISKIIEANISEHFKV-RDTNLSASRSESHHSQYL--------------WSPPPP 447
            +A   ++      N  ++F + +  +LS    ++  S                 W PP  
Sbjct: 1326 RAKSAKLLSSFALNYWQNFSIAQQRHLSDHPPQNSGSPTAAAVVQPSATTIPLPWKPPVT 1385

BLAST of Lag0022627 vs. ExPASy TrEMBL
Match: A0A2N9FR17 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS17540 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 7.9e-47
Identity = 147/556 (26.44%), Postives = 237/556 (42.63%), Query Frame = 0

Query: 1    MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
            +KLDM+KAY+RVEW+FL +IM+++GF + WI+ +  C+ +V +S+L+NG P     P+RG
Sbjct: 909  LKLDMNKAYDRVEWIFLEKIMSRMGFHQKWISLMTECISTVSYSILVNGEPHGYIKPSRG 968

Query: 61   LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
            L+QGDP+SPYLFLLCAEGL  L+N+       +G+ + +    I+HLFF DD+L+F +A 
Sbjct: 969  LRQGDPLSPYLFLLCAEGLHSLINKAGRDGDIQGVSLCRKGPKITHLFFADDNLLFSKAT 1028

Query: 121  ENDCSTIKGILETYEKASDQ---------------------------------------- 180
               C  I+GIL+ YEKAS Q                                        
Sbjct: 1029 PTACEKIQGILDQYEKASGQQVNRDKTTIYFSKNTPEASQNVIKEALEAKFFPHGTILNC 1088

Query: 181  ---------------------------------------DAEEILKIPLGSKNAMDEIIW 240
                                                   D E I +IPL +++  D++IW
Sbjct: 1089 PTTTPGSYAWQSLLKARELITKPQMTWDHSLIDSLFLPYDVEAIKQIPLSNQDHADKLIW 1148

Query: 241  AKHPKGSFSVKTAYHLAVNQEEHRKASCSDNSKIQAIWKNFWKIKAIPRAKICEQIGIK- 300
              +  G +SV++ Y   V++E+      S  ++     K   + + IP    CE  G K 
Sbjct: 1149 PGNTHGDYSVRSGYRFLVDEEDKNLPGSSRATREALPTKLNLQKRHIPIGTTCELCGKKD 1208

Query: 301  ----HAF---DMGVPAWDALLIW------EGLVERLE----------EEEISIAILILWN 360
                HA          WDA  +W        +V+  +          + E    I+I W 
Sbjct: 1209 EDTIHALWSCKQLQSVWDA-EVWIQPLRNSSMVDFADLLCKVLHLGRDSEPETFIIICWA 1268

Query: 361  IWTTRNKVINNGYKADQNQISKIIEANISEHFKVRDTNLSASRSESHHSQYLWSPPPPPF 420
            +W  RNK+         NQ+   ++  + E+   R+T  +  + +   +   W PP    
Sbjct: 1269 LWQRRNKIRLQQEVDSINQVDPKVKCYLEEY--TRETEQTKPQPQPAMT-VKWQPPRKLR 1328

Query: 421  LKLNSDASWSSSEEIGGLGWTVRDSSGSLVFAGNMKTKKNWETLELEALAMKEGLSSFLQ 454
             K+N D +        G+   VRDS G ++ +   K +       +EA A+K  +   L+
Sbjct: 1329 YKVNFDGAVFKETNEAGIRVIVRDSHGLVMASLTQKVRFPHSVPSIEAWAVKRAIQFVLE 1388

BLAST of Lag0022627 vs. ExPASy TrEMBL
Match: A0A803QHW9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.3e-46
Identity = 148/508 (29.13%), Postives = 232/508 (45.67%), Query Frame = 0

Query: 1   MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
           +KLDMSKA++RVEW FL  +M K+GF   WIA +  C+ S   S  +NG       P+RG
Sbjct: 52  LKLDMSKAFDRVEWTFLWAVMEKMGFASNWIALIQNCLTSSSLSFSLNGEVTGYVKPSRG 111

Query: 61  LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
           L+QGDP+SPY FL+ +EGLS LL  EE+    +G+R+ +   S+SHL F DDSL+FC   
Sbjct: 112 LRQGDPLSPYHFLIGSEGLSRLLQHEEAIGNLQGLRLTRRAPSVSHLLFADDSLLFCETT 171

Query: 121 ENDCSTIKGILETYEKASDQDAEEILKIPLGSKNAMDEIIWAKHPKGSFSVKTAYHLAVN 180
            N    I+  L  Y +ASD+                  +IW       ++VK+ +HLA  
Sbjct: 172 NNSALAIERALNLYHRASDR------------------LIWHHTTSRIYAVKSGFHLAAT 231

Query: 181 QEEHRKASCSD-NSKIQAIWKNFWKIK---------------------AIPRAKICEQI- 240
            +E ++AS SD NSK    WK+FW +K                     A+ R K+ + + 
Sbjct: 232 LDEQQEASASDLNSK---WWKSFWHLKLPSKIKIFAWKVIYSAIPIAAALHRRKVIDSVA 291

Query: 241 ---------GIKHAF-------------DMGVPAWDALLIWEG-----LVERLEEEEISI 300
                     I HA              D  +    A  + +G     L     + +  +
Sbjct: 292 CSTCSNAWESIGHALFTCPHAKSTWKHSDFTMDFRKAQAMHDGDFIFHLSNVHSQPDFEL 351

Query: 301 AILILWNIWTTRNKVINNGYKAD--------QNQISKIIEANISEHFKVRDTNLSASRSE 360
            +  +W IW+ RNKV++ G   +        Q+ + K   +    H     T++S + + 
Sbjct: 352 NVCTMWAIWSHRNKVLHGGVLKEGKITTSFAQDYLGKYHASTRQTHTAPAVTSVSPTGAH 411

Query: 361 SHHSQ----YLWSPPPPPFLKLNSDASWSSSEEIGGLGWTVRDSSGSLVFAGNMKTKKNW 420
           +   Q      W PP    LKLN DA+ +++ +  G+G  VRD +G +V A +   +  +
Sbjct: 412 TSKKQASKDISWQPPTAAGLKLNVDAAVNATSQTLGVGAIVRDHNGLVVAAISKPAQGCF 471

Query: 421 ETLELEALAMKEGLSSFLQNSACKDRPLVVESDSSTLIKLIQNQESDLSEVRSIVEEIGL 447
            + E+EA A+   L+  LQ      +   +E+DS  +   + +   DLS    ++ ++  
Sbjct: 472 HSDEMEAKALFHSLNWALQQQL---QITHIETDSLRVFNALIHTSIDLSCFLDLIIDVCC 531

BLAST of Lag0022627 vs. ExPASy TrEMBL
Match: A0A803QB90 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 4.3e-45
Identity = 156/647 (24.11%), Postives = 255/647 (39.41%), Query Frame = 0

Query: 1   MKLDMSKAYNRVEWVFLREIMAKIGFCEGWIAKVMMCVESVEFSVLINGVPQDPFMPNRG 60
           +KLDMSKA++RVEW +L  +M K+GF   W+  +M C+ +  FS  +NG       P+RG
Sbjct: 93  LKLDMSKAFDRVEWQYLEAVMLKMGFASWWVTLIMNCITTSSFSFSLNGDVVGHVQPSRG 152

Query: 61  LKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSISHLFFVDDSLVFCRAE 120
           L+QGDP+SPYLFL+C+EGLS +L  EES    RG+ I +   SISHL F DDSL+FC+A 
Sbjct: 153 LRQGDPLSPYLFLICSEGLSRILQFEESQRNLRGLSITRNAPSISHLLFADDSLLFCQAS 212

Query: 121 ENDCSTIKGILETYEKASDQ---------------------------------------- 180
           +     IK +L TY +AS Q                                        
Sbjct: 213 DQSALAIKKVLHTYHQASGQVLNNDKSVMSFSPNTSVATQNFFAQTLQMPITDCHEKYLG 272

Query: 181 -------DAEEILK-----------------IPLGSKNAM-------------------- 240
                  D +E+                     +G K  +                    
Sbjct: 273 LPSYSGRDKQELFSSIKEKVWKLLHSWNEKIFSIGGKEVLLKAVVQSIPTYAMSCFKLTK 332

Query: 241 ----------------------------------DEIIWAKHPKGSFSVKTAYHLAVNQE 300
                                             D +IW     G ++VK+ +HLA   E
Sbjct: 333 KFCNQLESMMANFWWGTNQNGTKIHWKRWNSLSDDRLIWHHSSNGIYNVKSGFHLATTLE 392

Query: 301 EHRKASCSDNSKIQAIWKNFWKIKAIPRAKIC-----------------EQIGIKHAFDM 360
           +   +S SD ++    WK FW +K  P+ +I                   +I    A  +
Sbjct: 393 DQLISSTSDVNR--DWWKYFWSLKLPPKIRIFAWKVFQEILPTAAGLFKRKIIDSAACSL 452

Query: 361 GVPAWDAL-----------LIWEGLVERLE---------------------EEEISIAIL 420
              +W+++            +W+    R++                     +E+  + + 
Sbjct: 453 CTSSWESIGHALFGCQHAKAVWKKSKFRIDFNKAQSMFKGDYLHYLSGVYNQEDFELFLC 512

Query: 421 ILWNIWTTRNKVINNGYKADQNQISKIIEANISEHFKVRDTNLSASRSESHHS------- 463
           +LW +WT RN+V + G   D + I        ++  K +      + +  +HS       
Sbjct: 513 LLWGVWTNRNRVFHGGKSRDTSSIIAYTTGFYNDFSKAKTAPKPVAVAGPNHSHQMASSP 572

BLAST of Lag0022627 vs. TAIR 10
Match: AT2G41710.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 7.1e-24
Identity = 64/81 (79.01%), Postives = 66/81 (81.48%), Query Frame = 0

Query: 463 MAS-SSSDPGFKHEAGACSAAAAGGGTAESSEVVMANDQLLLYRGLKKAKKERGCTAKER 522
           MAS SSSD G K EAG CS    GGG  ESSE V A+DQ+LLYRG KKAKKERGCTAKER
Sbjct: 1   MASVSSSDQGPKTEAG-CS----GGGGGESSETVAASDQMLLYRGFKKAKKERGCTAKER 60

Query: 523 ISKMPPCAAGKRSSIYRGVTR 543
           ISKMPPC AGKRSSIYRGVTR
Sbjct: 61  ISKMPPCTAGKRSSIYRGVTR 76

BLAST of Lag0022627 vs. TAIR 10
Match: AT2G41710.2 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 7.1e-24
Identity = 64/81 (79.01%), Postives = 66/81 (81.48%), Query Frame = 0

Query: 463 MAS-SSSDPGFKHEAGACSAAAAGGGTAESSEVVMANDQLLLYRGLKKAKKERGCTAKER 522
           MAS SSSD G K EAG CS    GGG  ESSE V A+DQ+LLYRG KKAKKERGCTAKER
Sbjct: 1   MASVSSSDQGPKTEAG-CS----GGGGGESSETVAASDQMLLYRGFKKAKKERGCTAKER 60

Query: 523 ISKMPPCAAGKRSSIYRGVTR 543
           ISKMPPC AGKRSSIYRGVTR
Sbjct: 61  ISKMPPCTAGKRSSIYRGVTR 76

BLAST of Lag0022627 vs. TAIR 10
Match: AT2G41710.3 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 7.1e-24
Identity = 64/81 (79.01%), Postives = 66/81 (81.48%), Query Frame = 0

Query: 463 MAS-SSSDPGFKHEAGACSAAAAGGGTAESSEVVMANDQLLLYRGLKKAKKERGCTAKER 522
           MAS SSSD G K EAG CS    GGG  ESSE V A+DQ+LLYRG KKAKKERGCTAKER
Sbjct: 1   MASVSSSDQGPKTEAG-CS----GGGGGESSETVAASDQMLLYRGFKKAKKERGCTAKER 60

Query: 523 ISKMPPCAAGKRSSIYRGVTR 543
           ISKMPPC AGKRSSIYRGVTR
Sbjct: 61  ISKMPPCTAGKRSSIYRGVTR 76

BLAST of Lag0022627 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 79.0 bits (193), Expect = 1.3e-14
Identity = 35/68 (51.47%), Postives = 46/68 (67.65%), Query Frame = 0

Query: 46  LINGVPQDPFMPNRGLKQGDPISPYLFLLCAEGLSGLLNREESSLKFRGIRINKICHSIS 105
           +ING PQ    P+RGL+QGDP+SPYLF+LC E LSGL  R +   +  GIR++     I+
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 106 HLFFVDDS 114
           HL F DD+
Sbjct: 73  HLLFADDT 80

BLAST of Lag0022627 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 1.1e-13
Identity = 53/217 (24.42%), Postives = 100/217 (46.08%), Query Frame = 0

Query: 254 ILWNIWTTRNKVINNGYKADQNQISKIIEANISE-HFKVRDTNLSASRSESHHSQYLWSP 313
           +LW +W  RN+++  G + +  ++ +  E ++ E   +    +       +  S   W P
Sbjct: 363 LLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSSCGRWRP 422

Query: 314 PPPPFLKLNSDASWSSSEEIGGLGWTVRDSSGSLVFAGNMKTKKNWETLELEALAMKEGL 373
           PP  ++K N+DA+W+   E  G+GW +R+  G + + G     K    LE E  AM+  +
Sbjct: 423 PPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAV 482

Query: 374 SSFLQNSACKDRPLVVESDSSTLIKLIQNQESDLSEVRSIVEEIGLLARSAIGISFVWCS 433
            S    S  +   ++ ESDS  LI+++ N E     ++  ++++  L      + FV+  
Sbjct: 483 LSL---SRFQYNYVIFESDSQVLIEILNNDEI-WPSLKPTIQDLQRLLSQFTEVKFVFIP 542

Query: 434 RSCNRATHTLAKDARLAGTIEFPLIFVDVSMASSSSD 470
           R  N     +A+++      +  L  +  S A SS D
Sbjct: 543 REGNTLAERVARESLSFLNYDPKLYSIVPSWARSSMD 575

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_030477911.19.6e-4724.88uncharacterized protein LOC115694948 [Cannabis sativa][more]
GAU36089.12.0e-4429.17hypothetical protein TSUD_277000 [Trifolium subterraneum][more]
XP_022157437.12.2e-4355.90uncharacterized protein LOC111024135 [Momordica charantia][more]
BAH93889.12.9e-4330.04Os07g0417700 [Oryza sativa Japonica Group][more]
XP_030502823.11.1e-4225.77uncharacterized protein LOC115717993 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
Q8GWK21.0e-2279.01AP2-like ethylene-responsive transcription factor At2g41710 OS=Arabidopsis thali... [more]
P925551.9e-1351.47Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ... [more]
X5HYT81.1e-0848.42AP2-like ethylene-responsive transcription factor SMOS1 OS=Oryza sativa subsp. j... [more]
P113691.1e-0522.39LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
Q051187.2e-0527.83Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra... [more]
Match NameE-valueIdentityDescription
A0A803NML19.0e-5127.95Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803PM529.3e-4827.50Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A2N9FR177.9e-4726.44Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A803QHW92.3e-4629.13Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QB904.3e-4524.11Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G41710.17.1e-2479.01Integrase-type DNA-binding superfamily protein [more]
AT2G41710.27.1e-2479.01Integrase-type DNA-binding superfamily protein [more]
AT2G41710.37.1e-2479.01Integrase-type DNA-binding superfamily protein [more]
ATMG01250.11.3e-1451.47RNA-directed DNA polymerase (reverse transcriptase) [more]
AT4G29090.11.1e-1324.42Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 318..450
e-value: 1.4E-10
score: 43.2
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 321..446
e-value: 3.5E-21
score: 75.3
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 1..133
e-value: 2.9E-16
score: 59.7
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 1..241
score: 8.773122
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 1..140
NoneNo IPR availablePANTHERPTHR33116:SF45SUBFAMILY NOT NAMEDcoord: 1..140
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 320..444
e-value: 2.93974E-18
score: 78.8952
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 2..134
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 318..449

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0022627.1Lag0022627.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity