Lag0022504 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0022504
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr7: 31060332 .. 31061162 (-)
RNA-Seq ExpressionLag0022504
SyntenyLag0022504
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACCGATCGAAAAAAAAACGCGTGAGTTGATGGCTGACGCTGCTGCAGACAACATTAATGAGCTACCTGCAGCAGCCCTTGGGAAGACCATGACCGATGGTCTTCCCAAGGAAAATAATTACTTTTCTTTTATTTTTTTTTTCAATTTCTTGTATCTTCGATTATCTCTCTCACATGCACCATTCTTTCTTCTTTTTTTTTTTCAATTTCTTGTATCTCTGATTCTCTCTCTCACATGCACCATTCTTTCTTTTTTATTTTTTTATTTTTTTTCAATTTCTTGTATCTCCGATTCTCTCTCTCGCAAGCACCATTCTTTCTATTTTTCTTTTCTTCAAACCTCTCAGATTCTTCATTTTTCTTTTCATCTTCTTCTTCTTTCTTTTTCAACTTTCTCTTTTCACTCTCTCAGTACCGACCATCAACCATCTTCACCTCGCCACTCTCTAAACTACCTGGATGAATTCAAAGCAACAAGCATAAAGGAGAATCAAGTAAGAAATACCGTAGATCAGAATAGAGGGAATCCAAAAGCAACAGACTTCACCAGCACAAGAAATATTCTAGAACAGAAGGAAAGGTGGTTGACGCCGGAAAGGAATACTTACAAGGTTAATATCGATGTTTCTGTCAAAGATGGAGTGCACGACAGAGGAGTTATTATTCAGAACGAAGACGAAGAAGTGATGGTAGCAACTCAGTCCGTGGGTCCATTTCTGGGATCAATAGAAATGGCCAAAGCTCTAGCCATCCGTGAAGGGGTTGAACTAGCAAAATCAACGGGAATCCATCCAATTTGGGTGGAAACCGATTCGCTCCCAGTGTGA

mRNA sequence

ATGGAACCGATCGAAAAAAAAACGCGTGAGTTGATGGCTGACGCTGCTGCAGACAACATTAATGAGCTACCTGCAGCAGCCCTTGGGAAGACCATGACCGATGCAACAAGCATAAAGGAGAATCAAGTAAGAAATACCGTAGATCAGAATAGAGGGAATCCAAAAGCAACAGACTTCACCAGCACAAGAAATATTCTAGAACAGAAGGAAAGGTGGTTGACGCCGGAAAGGAATACTTACAAGGTTAATATCGATGTTTCTGTCAAAGATGGAGTGCACGACAGAGGAGTTATTATTCAGAACGAAGACGAAGAAGTGATGGTAGCAACTCAGTCCGTGGGTCCATTTCTGGGATCAATAGAAATGGCCAAAGCTCTAGCCATCCGTGAAGGGGTTGAACTAGCAAAATCAACGGGAATCCATCCAATTTGGGTGGAAACCGATTCGCTCCCAGTGTGA

Coding sequence (CDS)

ATGGAACCGATCGAAAAAAAAACGCGTGAGTTGATGGCTGACGCTGCTGCAGACAACATTAATGAGCTACCTGCAGCAGCCCTTGGGAAGACCATGACCGATGCAACAAGCATAAAGGAGAATCAAGTAAGAAATACCGTAGATCAGAATAGAGGGAATCCAAAAGCAACAGACTTCACCAGCACAAGAAATATTCTAGAACAGAAGGAAAGGTGGTTGACGCCGGAAAGGAATACTTACAAGGTTAATATCGATGTTTCTGTCAAAGATGGAGTGCACGACAGAGGAGTTATTATTCAGAACGAAGACGAAGAAGTGATGGTAGCAACTCAGTCCGTGGGTCCATTTCTGGGATCAATAGAAATGGCCAAAGCTCTAGCCATCCGTGAAGGGGTTGAACTAGCAAAATCAACGGGAATCCATCCAATTTGGGTGGAAACCGATTCGCTCCCAGTGTGA

Protein sequence

MEPIEKKTRELMADAAADNINELPAAALGKTMTDATSIKENQVRNTVDQNRGNPKATDFTSTRNILEQKERWLTPERNTYKVNIDVSVKDGVHDRGVIIQNEDEEVMVATQSVGPFLGSIEMAKALAIREGVELAKSTGIHPIWVETDSLPV
Homology
BLAST of Lag0022504 vs. NCBI nr
Match: GAY60770.1 (hypothetical protein CUMW_204620 [Citrus unshiu])

HSP 1 Score: 65.1 bits (157), Expect = 6.0e-07
Identity = 31/89 (34.83%), Postives = 54/89 (60.67%), Query Frame = 0

Query: 64  NILEQKERWLTPERNTYKVNIDVSV--KDGVHDRGVIIQNEDEEVMVATQSVGPFLGSIE 123
           ++  ++ERW  P++N +K+N D ++   +G+   GV+I+N  EE    +    PF G IE
Sbjct: 10  SLASKEERWSPPDKNWFKLNSDATIDETEGLARLGVVIRNSREEFTATSICRRPFSGVIE 69

Query: 124 MAKALAIREGVELAKSTGIHPIWVETDSL 151
             +A A+ EG+EL    G+ P+ +E+DS+
Sbjct: 70  FVEASAVLEGIELVVDLGLTPLMIESDSV 98

BLAST of Lag0022504 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 60.5 bits (145), Expect = 1.5e-05
Identity = 34/99 (34.34%), Postives = 55/99 (55.56%), Query Frame = 0

Query: 53  NPKATDFTSTRNILEQKERWLTPERNTYKVNIDVS--VKDGVHDRGVIIQNEDEEVMVAT 112
           NP     T+T  +L     WL P++  YK+N D S    D     G+II+N+  +VM + 
Sbjct: 20  NPFPGRVTNTAEVL-----WLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASA 79

Query: 113 QSVGPFLGSIEMAKALAIREGVELAKSTGIHPIWVETDS 150
                 + S++MA+A+   EG++LA   G++P+ +ETDS
Sbjct: 80  TKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDS 113

BLAST of Lag0022504 vs. NCBI nr
Match: ESR35143.1 (hypothetical protein CICLE_v10007098mg, partial [Citrus clementina])

HSP 1 Score: 60.5 bits (145), Expect = 1.5e-05
Identity = 32/78 (41.03%), Postives = 50/78 (64.10%), Query Frame = 0

Query: 77  RNTYKVNIDVSVK--DGVHDRGVIIQNEDEEVMVATQSVGPFLGSIEMAKALAIREGVEL 136
           +N +K+N D ++   DG+   GV+I+N   E M A+     F G IE+A+A AI EG++L
Sbjct: 1   KNWFKLNSDAAINEADGLAGLGVVIRNSKGEFMTASNYRRRFFGDIELAEASAILEGIKL 60

Query: 137 AKSTGIHPIWVETDSLPV 153
           A+  G+ P+ VE+DS+ V
Sbjct: 61  AEDLGLTPLVVESDSINV 78

BLAST of Lag0022504 vs. NCBI nr
Match: XP_024037110.1 (uncharacterized protein LOC112097050 [Citrus clementina] >XP_024037144.1 uncharacterized protein LOC112097050 [Citrus clementina] >XP_024037175.1 uncharacterized protein LOC112097050 [Citrus clementina])

HSP 1 Score: 60.1 bits (144), Expect = 1.9e-05
Identity = 32/80 (40.00%), Postives = 47/80 (58.75%), Query Frame = 0

Query: 72  WLTPERNTYKVNIDVSV--KDGVHDRGVIIQNEDEEVMVATQSVGPFLGSIEMAKALAIR 131
           W  P+   YK+N D ++  + GV   G II+N    VM A  S  P+ G +E+A+ALAI 
Sbjct: 148 WHPPDPPRYKLNTDAALDKEGGVTGLGCIIRNHQGAVMAAVTSKKPYFGDVEIAEALAIL 207

Query: 132 EGVELAKSTGIHPIWVETDS 150
           EG++LA    + P  +E+DS
Sbjct: 208 EGLKLAAEVALSPHLIESDS 227

BLAST of Lag0022504 vs. NCBI nr
Match: XP_024039172.1 (uncharacterized protein LOC112097823 [Citrus clementina])

HSP 1 Score: 60.1 bits (144), Expect = 1.9e-05
Identity = 31/81 (38.27%), Postives = 50/81 (61.73%), Query Frame = 0

Query: 72  WLTPERNTYKVNIDVS--VKDGVHDRGVIIQNEDEEVMVATQSVGPFLGSIEMAKALAIR 131
           W  P+   +K+N DV+  V++G    G +I+N +  VM+A    G +   + +A+A AIR
Sbjct: 41  WEPPQLGWFKLNTDVAVDVQNGKVSYGAVIRNHEGLVMLAGTDFGNYCEDVAIAEAEAIR 100

Query: 132 EGVELAKSTGIHPIWVETDSL 151
            G+ LAK TG+ P+ +E+DSL
Sbjct: 101 FGIRLAKETGLFPLMIESDSL 121

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GAY60770.16.0e-0734.83hypothetical protein CUMW_204620 [Citrus unshiu][more]
XP_022140628.11.5e-0534.34uncharacterized protein LOC111011237 [Momordica charantia][more]
ESR35143.11.5e-0541.03hypothetical protein CICLE_v10007098mg, partial [Citrus clementina][more]
XP_024037110.11.9e-0540.00uncharacterized protein LOC112097050 [Citrus clementina] >XP_024037144.1 unchara... [more]
XP_024039172.11.9e-0538.27uncharacterized protein LOC112097823 [Citrus clementina][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 85..152
e-value: 1.5E-8
score: 34.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..63
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 82..149
e-value: 3.97931E-9
score: 49.62

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0022504.1Lag0022504.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity