Lag0022145 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0022145
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr7: 19714637 .. 19717127 (+)
RNA-Seq Expression	Lag0022145
Synteny	Lag0022145

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAGATTAGGGTTTCTGAAGTGGGTTTTGTTGGTCGGGGTTTTGGGTTGCTGTGTTGAGGGTCTTGGGGTTAATTGGGGAACCATGGCCAATCATAAATTGCCGCCTAAGACCATTGTGCAGATGTTTAAGGATAATGGGATTCAGAAAGTGAAGCTGTTTGATGCAGATCAGTCCACCATGGGAGCTCTGGCTGGGACTGGTATTGAGGTCATGGTCGCCATTCCTAATGATCAGCTTGCCTCCATGGGAGACTACAACCGAGCTAAGCAATGGGTGCAGAGGAACGTCACCCGTTACAACTTTGATGGAGGAGTTATCATCAAGTAATTCATTCCACTTTCTATATCTGTTTCAAACCATAGAAAATTGATTGTGTTTACTTGGTTTCGATTCTTTTCTGCTTCAAAGCCTGTTCTGTTTCTCTCTGAATCTTGCAAATCCATAATCATGTTATTTCCACATCTGAGGAAGTCATTGAGCTTGCATATAGGTGGTCAACTTTGCAATCATTTGGATGGTTGTTCGAACTTATTTATTTAGCGTTGAAATAAACCTCTTCTATTTATTAGCAATCTGAGGAACACGTTAGAATTTTATTTTTCTGAGTTGAGTTTTGGAAATAGATATTTAAGTTTTTTTTCCCCTTAAGCATTGACTTTTCTGATTAAGTAAATCGTAAAACAATATATAAATCGTTGGCTTTCCAGTTTCAGCAAAGGGTGGAAAGTTCTTTAACTATTTAAAAACTGGTCGCGGATGTGGTTTACGTTGACCAAGAACCCTTGAATGGGTTGATCTTTTCTGCCAATGAATGCAGCTCAGCTGTTTTTCTATGAAATTCCATGTTCTGTTTCATCAAATAGATCAATAATATGTATAATTGTAGTGCAGGACAAATCTTTTCCTTAAATAGATAGCTGTCGTTTTCTTAGTTATTAAATACCAAAGGTTCTACTTAAAAGTTCATTTAGCAATTCTTGTGCAATTATAATTTTATCACTTTGGATGGAACTAGTTTTCATACCATTAAGTCTTTAAGGTATAATAGCCTTTTTGGTGGAGATTCTCGAATTTCTCGCTGCTCCAACTTCACCCAAGTTCTTTCAAGGAATACATTTGAATGATGTAATATTGCACCAGCTTGCACATAAATAAATAAATCGGAAAAAAAAAAAAAAAACAAAAGAGAGAAACTGTGTTTGAGTACTTATCTTTGATAAAAAAATCTTGGGAAGTTACATTTTATCTTCAATTCGAGTTCACTTGCTGTTTATGCTTAAATTAAGAACAAAGATATCGAATGGGTTTGTGATTGATGATTTTTTCTTGGATAGCAGAAGACTAACCTTTACTTGCTATGTTGTTCTTGTACAGATACGTTGCTGTTGGCAATGAACCATTTCTGTCATCCTATAATGGATCATTCTTGAATGTTACCTTTCCAGCCCTTCAGAACATTCAAAATGCACTCAATGAAGCAGGTCTTGGAGACTCCATCAAGGCGACGGTGCCCTTAAATGCTGATGTTTACAACTCCCCGGTTAATAGCCCGTATCCATCTGCTGGTAGGTTTCGCAGTGATATTAATGGACTCATGACCGAAATTGTCCAGTTTCTAAGCAAGAACAATGCACCTTTTACTGTAAACATTTATCCTTTCCTTAGTCTTTATGGCAACGATAACTTCCCTTTCGACTATGCCTTCTTCGATGGTGCAAGTAATCCTGTTGTTGATATTGGCACTGGGATTCAGTATACCAATGTTTTCGATGCCAACTTTGACACCTTGGTTTCGTCTCTGAAAGCTGTTGGACTTGGGGACATGCCCATTCTAGTTGGCGAGGTTGGATGGCCAACTGATGGTGACAAGAATGCCAATGTCGGTAATGCTTATAGATTCTACAATGGCCTTCTACCAAGGCTTGCAGCCAAGAAGGGCACCCCACTGAGGTCTGGATTCATTGAAGTATACTTGTTCAGTTTTCTGGATGAAAATGGCAAGAGCATTGCTCCAGGAAGTTTCGAGCGACACTGGGGTATTTTCCAGTATGACGGTCAGCCAAAGTTTGCTATGGATCTTTCTGGTCAGGGCCAAAACAAACTACTTATTGGAGCACAGAATGTGCAGTATCTTCCTCAAAAGTGGTGTATGTTCAATCCAAATGCAAAGGATCTAAGTAAACTTGCTGATAATATAGATTATGCGTGCACCTTTTCGGATTGCACTTCTCTTGGATATGGATCTTCTTGCAATAATTTGGACGCTAATGGGAATGCATCATATGCATTTAATATGTATTTCCAGGTGCAAAATCAAGATGAAATGGCCTGTAATTTTCAAGGTTTAGCCACAATGACCTCACAGAATCTTTCGCAAGGTACTTGTAATTTCATCATCCAGATAGCTTCCTCGCCCCGGTCCTTCGCTGCTTCATTTGTTAGTTTAGCATCTATGGTCTTGTTATTCACAGCATTGTTGCTATGA

mRNA sequence

ATGGGAAGATTAGGGTTTCTGAAGTGGGTTTTGTTGGTCGGGGTTTTGGGTTGCTGTGTTGAGGGTCTTGGGGTTAATTGGGGAACCATGGCCAATCATAAATTGCCGCCTAAGACCATTGTGCAGATGTTTAAGGATAATGGGATTCAGAAAGTGAAGCTGTTTGATGCAGATCAGTCCACCATGGGAGCTCTGGCTGGGACTGGTATTGAGGTCATGGTCGCCATTCCTAATGATCAGCTTGCCTCCATGGGAGACTACAACCGAGCTAAGCAATGGGTGCAGAGGAACGTCACCCGTTACAACTTTGATGGAGGAGTTATCATCAAATACGTTGCTGTTGGCAATGAACCATTTCTGTCATCCTATAATGGATCATTCTTGAATGTTACCTTTCCAGCCCTTCAGAACATTCAAAATGCACTCAATGAAGCAGGTCTTGGAGACTCCATCAAGGCGACGGTGCCCTTAAATGCTGATGTTTACAACTCCCCGGTTAATAGCCCGTATCCATCTGCTGGTAGGTTTCGCAGTGATATTAATGGACTCATGACCGAAATTGTCCAGTTTCTAAGCAAGAACAATGCACCTTTTACTGTAAACATTTATCCTTTCCTTAGTCTTTATGGCAACGATAACTTCCCTTTCGACTATGCCTTCTTCGATGGTGCAAGTAATCCTGTTGTTGATATTGGCACTGGGATTCAGTATACCAATGTTTTCGATGCCAACTTTGACACCTTGGTTTCGTCTCTGAAAGCTGTTGGACTTGGGGACATGCCCATTCTAGTTGGCGAGGTTGGATGGCCAACTGATGGTGACAAGAATGCCAATGTCGGTAATGCTTATAGATTCTACAATGGCCTTCTACCAAGGCTTGCAGCCAAGAAGGGCACCCCACTGAGGTCTGGATTCATTGAAGTATACTTGTTCAGTTTTCTGGATGAAAATGGCAAGAGCATTGCTCCAGGAAGTTTCGAGCGACACTGGGGTATTTTCCAGTATGACGGTCAGCCAAAGTTTGCTATGGATCTTTCTGGTCAGGGCCAAAACAAACTACTTATTGGAGCACAGAATGTGCAGTATCTTCCTCAAAAGTGGTGTATGTTCAATCCAAATGCAAAGGATCTAAGTAAACTTGCTGATAATATAGATTATGCGTGCACCTTTTCGGATTGCACTTCTCTTGGATATGGATCTTCTTGCAATAATTTGGACGCTAATGGGAATGCATCATATGCATTTAATATGTATTTCCAGGTGCAAAATCAAGATGAAATGGCCTGTAATTTTCAAGGTTTAGCCACAATGACCTCACAGAATCTTTCGCAAGGTACTTGTAATTTCATCATCCAGATAGCTTCCTCGCCCCGGTCCTTCGCTGCTTCATTTGTTAGTTTAGCATCTATGGTCTTGTTATTCACAGCATTGTTGCTATGA

Coding sequence (CDS)

ATGGGAAGATTAGGGTTTCTGAAGTGGGTTTTGTTGGTCGGGGTTTTGGGTTGCTGTGTTGAGGGTCTTGGGGTTAATTGGGGAACCATGGCCAATCATAAATTGCCGCCTAAGACCATTGTGCAGATGTTTAAGGATAATGGGATTCAGAAAGTGAAGCTGTTTGATGCAGATCAGTCCACCATGGGAGCTCTGGCTGGGACTGGTATTGAGGTCATGGTCGCCATTCCTAATGATCAGCTTGCCTCCATGGGAGACTACAACCGAGCTAAGCAATGGGTGCAGAGGAACGTCACCCGTTACAACTTTGATGGAGGAGTTATCATCAAATACGTTGCTGTTGGCAATGAACCATTTCTGTCATCCTATAATGGATCATTCTTGAATGTTACCTTTCCAGCCCTTCAGAACATTCAAAATGCACTCAATGAAGCAGGTCTTGGAGACTCCATCAAGGCGACGGTGCCCTTAAATGCTGATGTTTACAACTCCCCGGTTAATAGCCCGTATCCATCTGCTGGTAGGTTTCGCAGTGATATTAATGGACTCATGACCGAAATTGTCCAGTTTCTAAGCAAGAACAATGCACCTTTTACTGTAAACATTTATCCTTTCCTTAGTCTTTATGGCAACGATAACTTCCCTTTCGACTATGCCTTCTTCGATGGTGCAAGTAATCCTGTTGTTGATATTGGCACTGGGATTCAGTATACCAATGTTTTCGATGCCAACTTTGACACCTTGGTTTCGTCTCTGAAAGCTGTTGGACTTGGGGACATGCCCATTCTAGTTGGCGAGGTTGGATGGCCAACTGATGGTGACAAGAATGCCAATGTCGGTAATGCTTATAGATTCTACAATGGCCTTCTACCAAGGCTTGCAGCCAAGAAGGGCACCCCACTGAGGTCTGGATTCATTGAAGTATACTTGTTCAGTTTTCTGGATGAAAATGGCAAGAGCATTGCTCCAGGAAGTTTCGAGCGACACTGGGGTATTTTCCAGTATGACGGTCAGCCAAAGTTTGCTATGGATCTTTCTGGTCAGGGCCAAAACAAACTACTTATTGGAGCACAGAATGTGCAGTATCTTCCTCAAAAGTGGTGTATGTTCAATCCAAATGCAAAGGATCTAAGTAAACTTGCTGATAATATAGATTATGCGTGCACCTTTTCGGATTGCACTTCTCTTGGATATGGATCTTCTTGCAATAATTTGGACGCTAATGGGAATGCATCATATGCATTTAATATGTATTTCCAGGTGCAAAATCAAGATGAAATGGCCTGTAATTTTCAAGGTTTAGCCACAATGACCTCACAGAATCTTTCGCAAGGTACTTGTAATTTCATCATCCAGATAGCTTCCTCGCCCCGGTCCTTCGCTGCTTCATTTGTTAGTTTAGCATCTATGGTCTTGTTATTCACAGCATTGTTGCTATGA

Protein sequence

MGRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTMGALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASFVSLASMVLLFTALLL

Homology

[Close]

BLAST of Lag0022145 vs. NCBI nr
Match: XP_038903901.1 (glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida])

HSP 1 Score: 930.2 bits (2403), Expect = 6.9e-267
Identity = 454/479 (94.78%), Postives = 467/479 (97.49%), Query Frame = 0


Query: 1   MGRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRLGFLKWVLLVGVLGC VEGLGVNWGTMANH+LPPKTIVQMFKDNGI+KVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGCYVEGLGVNWGTMANHRLPPKTIVQMFKDNGIKKVKLFDADQS 60

Query: 61  TMGALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFL 120
           +MGALAGTGIEVMVAIPNDQL++MGDYNRAKQWVQRNV+RY FDGGV IKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           NGLMT+IVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD+GTGIQYTNV
Sbjct: 181 NGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDVGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTP 300
           FDANFDTLVSSLKAV LGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA KGTP
Sbjct: 241 FDANFDTLVSSLKAVELGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNV 360
           LR GFIEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKFAMDLSGQGQNKLL+GAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFAMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLP+KWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPKKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASFVSLASMVLLFTALLL 480
           VQNQDE+ACNFQGLATMTSQNLSQGTCNFIIQ+ASSP S AASFVSLASM LLFTALLL
Sbjct: 421 VQNQDELACNFQGLATMTSQNLSQGTCNFIIQLASSPSSQAASFVSLASMALLFTALLL 479

[Close]

BLAST of Lag0022145 vs. NCBI nr
Match: XP_004141142.1 (glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN59764.1 hypothetical protein Csa_001412 [Cucumis sativus])

HSP 1 Score: 921.8 bits (2381), Expect = 2.5e-264
Identity = 449/479 (93.74%), Postives = 463/479 (96.66%), Query Frame = 0


Query: 1   MGRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MG LGFLKWVLLVGVLG CVEGLGVNWGTMANH+LPPKT+VQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGSLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  TMGALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFL 120
           +MGALAGTGIEVMVAIPNDQL++MGDYNRAKQWVQRNVTRY FDGGV IKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180
           SSYNGSFLNVTFPAL NIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALLNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180

Query: 181 NGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LMT+IVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMTDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKN NVGNAYRFYNGLLPRLAA KGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNGNVGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNV 360
           LR GFIEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKF MDLSGQGQNKLL+GAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASFVSLASMVLLFTALLL 480
           VQNQDE+ACNFQGLAT+TSQNLSQGTCNFIIQ+ASSP S AASFVSLASM+LLF ALLL
Sbjct: 421 VQNQDELACNFQGLATVTSQNLSQGTCNFIIQLASSPPSHAASFVSLASMILLFIALLL 479

[Close]

BLAST of Lag0022145 vs. NCBI nr
Match: XP_008465328.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo] >KAA0051277.1 glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo var. makuwa] >TYK29984.1 glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo var. makuwa])

HSP 1 Score: 919.1 bits (2374), Expect = 1.6e-263
Identity = 449/479 (93.74%), Postives = 463/479 (96.66%), Query Frame = 0


Query: 1   MGRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRLGFLKWVLLVGVLG CVEGLGVNWGTMANH+LPPKT+VQM KDNGIQKVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQS 60

Query: 61  TMGALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFL 120
           +MGALAGTGIEVMVAIPNDQL++MGDYNRAKQWVQRNVTRY FDGGV IKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LM +IVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA KGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNV 360
           LR GFIEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKF MDLSGQGQNKLL+GAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           +YLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 EYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASFVSLASMVLLFTALLL 480
           VQNQDE+ACNFQGLAT+TS+NLSQGTCNFIIQ+ASSP S AASFVSLASMVLLF ALLL
Sbjct: 421 VQNQDELACNFQGLATITSRNLSQGTCNFIIQLASSPPSHAASFVSLASMVLLFIALLL 479

[Close]

BLAST of Lag0022145 vs. NCBI nr
Match: XP_023526667.1 (glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 917.9 bits (2371), Expect = 3.6e-263
Identity = 446/479 (93.11%), Postives = 461/479 (96.24%), Query Frame = 0


Query: 1   MGRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRL  LKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60

Query: 61  TMGALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFL 120
           +MGALAGTGIEVMVAIPNDQL +MGDYNRAKQWVQRNV+RY FDGGV IKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG G++IKATVPLNADVYNSPVN+PYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGETIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           NGLMT+IVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD+ TGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA KGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNV 360
           LR GFIEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKF MDLSGQGQNKLL+GAQ+V
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASFVSLASMVLLFTALLL 480
           VQNQDE+ACNFQGLAT+TSQNLSQGTC FIIQ+ASS  SF ASFVSL SM LLFTA+LL
Sbjct: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSFDASFVSLVSMALLFTAMLL 479

[Close]

BLAST of Lag0022145 vs. NCBI nr
Match: XP_022155900.1 (glucan endo-1,3-beta-glucosidase 8-like [Momordica charantia])

HSP 1 Score: 917.5 bits (2370), Expect = 4.7e-263
Identity = 446/478 (93.31%), Postives = 465/478 (97.28%), Query Frame = 0


Query: 3   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTM 62
           RLGFLKWVLLVGVLGCCVEGLGVNWGTMANH+LPPKT+VQMFKDNGI+KVKLFDADQSTM
Sbjct: 4   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTM 63

Query: 63  GALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSS 122
           GALAG+GIEVMVAIPNDQLA+MGDY RA+QWVQRNVTRYNFDGGV+IKYVAVGNEPFLSS
Sbjct: 64  GALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSS 123

Query: 123 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 182
           YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING
Sbjct: 124 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 183

Query: 183 LMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFD 242
           LMT+IVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD G GIQYTNVFD
Sbjct: 184 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFD 243

Query: 243 ANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLR 302
           ANFDTLVSSLKAVG GDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAA KGTPLR
Sbjct: 244 ANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLR 303

Query: 303 SGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQY 362
           +G+IEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKF MDLSGQGQNKLL+GAQ+VQY
Sbjct: 304 AGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 363

Query: 363 LPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 422
           LP+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ
Sbjct: 364 LPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423

Query: 423 NQDEMACNFQGLATMTSQNLSQGTCNFIIQI-ASSPRSFAASFVSLASMVLLFTALLL 480
           NQDEMACNFQGLAT+TSQNLSQGTCNFIIQ+ +SSP   AASFVSLASMV+L TAL L
Sbjct: 424 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALXL 481

[Close]

BLAST of Lag0022145 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 732.6 bits (1890), Expect = 2.8e-210
Identity = 356/467 (76.23%), Postives = 402/467 (86.08%), Query Frame = 0


Query: 9   WVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTMGALAGT 68
           +V+++G LG  V GLGVNWGTMA HKLPPKT+VQM KDN I KVKLFDAD++TMGALAG+
Sbjct: 11  FVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 70

Query: 69  GIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSSYNGSFL 128
           G+EVMVAIPNDQL  M  Y+RAK WV++NVTRYNFDGGV I +VAVGNEPFL SYNGSF+
Sbjct: 71  GLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFI 130

Query: 129 NVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTEIV 188
           N+TFPAL NIQNALNEAGLG+S+KATVPLNADVY+SP ++P PSAGRFR DI G MT+IV
Sbjct: 131 NLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIV 190

Query: 189 QFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTL 248
            FL KNNAP T+NIYPFLSLYGND+FP +YAFFDGA  P+ D   GI YTNVFDANFDTL
Sbjct: 191 DFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANFDTL 250

Query: 249 VSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLRSGFIEV 308
           VSSLKAVG GDMPI+VGEVGWPT+GDK+AN G+AYRFYNGLLPRL   KGTPLR  +IEV
Sbjct: 251 VSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEV 310

Query: 309 YLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQYLPQKWC 368
           YLF  LDE+ KSIAPG FERHWGIF++DGQPKF +DLSGQGQ+K LIGAQNV YLP KWC
Sbjct: 311 YLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWC 370

Query: 369 MFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMA 428
            FNP AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQDE A
Sbjct: 371 TFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESA 430

Query: 429 CNFQGLATMTSQNLSQGTCNFIIQI-ASSPRSFAASFVSLASMVLLF 475
           C FQGLAT+T+QN+SQG CNF IQI ASS  SF+ S  SL  +++ F
Sbjct: 431 CYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWF 474

[Close]

BLAST of Lag0022145 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.3e-135
Identity = 253/479 (52.82%), Postives = 330/479 (68.89%), Query Frame = 0


Query: 6   FLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTMGAL 65
           F    LL   L   VEG+GVNWG+ A H LPP T+V++ ++NGIQKVKLF+AD + + AL
Sbjct: 10  FFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKAL 69

Query: 66  AGTGIEVMVAIPNDQLASM-GDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSSYN 125
           + TGI+VMV IPND LA + G    A++WV +NV+ +    GV I+YVAVGNEPFL ++N
Sbjct: 70  SRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFN 129

Query: 126 GSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLM 185
           G+F  +T PALQNIQ+A+ +AGL   +K TVPLNADVY S  N   PS G FR +I  LM
Sbjct: 130 GTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASN--LPSDGDFRPEIRDLM 189

Query: 186 TEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDAN 245
             IV+FLS N APFT+NIYPF+SLY + NFP ++AFFDG   P+ D   G  Y NV DAN
Sbjct: 190 LNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPIND--NGRIYDNVLDAN 249

Query: 246 FDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLRSG 305
           +DTLV SL+  G G++ I+VGEVGWPTDGDKNAN+  A R+  G + R  A KGTP+R G
Sbjct: 250 YDTLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPG 309

Query: 306 FIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDL-SGQGQNKLLIGAQNVQYL 365
            ++ YLF  +DE+ KSI PG+FERHWGIF  DGQPK+ + L SG G    LI A++V YL
Sbjct: 310 AMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLGSGNG----LIPAKDVHYL 369

Query: 366 PQKWCMFNPNAK-DLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 425
            +KWC+  PNA     +L  ++ YAC  +DCTSLGYGSSC NL+   N SYAFN Y+QV 
Sbjct: 370 AKKWCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVS 429

Query: 426 NQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASF--VSLASMVLLFTALLL 480
           NQ + AC F GL+ +++++ S G+C F I I S   S A++   ++ ++ VLL  ++ L
Sbjct: 430 NQLDSACKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSICL 480

[Close]

BLAST of Lag0022145 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 479.6 bits (1233), Expect = 4.2e-134
Identity = 238/454 (52.42%), Postives = 320/454 (70.48%), Query Frame = 0


Query: 4   LGFLKWVLLVGVLGCC--VEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQST 63
           +G+   +LL+ V   C     +G NWGT A+H LPP  +V+M ++NGIQKVKLFDA+  T
Sbjct: 1   MGWGSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDT 60

Query: 64  MGALAGTGIEVMVAIPNDQLASM-GDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFL 123
           + AL  +GIEVMV IPN+ LA++      A++WV +NV+ +     V I+YVAVGNEPFL
Sbjct: 61  LRALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFL 120

Query: 124 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 183
           S+YNGS+L+ TFPAL+NIQ A+ +AGL + +K T PLNADVY+S  ++ +PS G FR++I
Sbjct: 121 STYNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDS--STTFPSGGDFRANI 180

Query: 184 NGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 243
             LM  IV+FLS+N  PFTVNIYP++SLY N +FP DYAFFDG + P+ D GT   Y N+
Sbjct: 181 RDLMITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGT--FYYNM 240

Query: 244 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTP 303
           FDAN+DTLV +L+  G G+MPI++GE+GWPTDGD NAN+  A +F  G +  ++  KGTP
Sbjct: 241 FDANYDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTP 300

Query: 304 LRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNV 363
            R G I+ YLFS +DE+ KS+ PG FERHWGIF +DG PK+A++L G      LI A+ V
Sbjct: 301 RRPGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGV 360

Query: 364 QYLPQKWCMFNPNAK-DLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYF 423
           +YL +KWC+  PN + D  ++A  + YAC+  DCTSLG G+SC NLD   N SYAFN Y+
Sbjct: 361 RYLERKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYY 420

Query: 424 QVQNQDEMACNFQGLATMTSQNLSQGTCNFIIQI 454
           Q+Q+Q + AC F  ++ +T  + S GTC F I I
Sbjct: 421 QIQDQLDTACKFPNISEVTKTDPSTGTCRFPIMI 449

[Close]

BLAST of Lag0022145 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 2.3e-95
Identity = 197/466 (42.27%), Postives = 275/466 (59.01%), Query Frame = 0


Query: 13  VGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTMGALAGTGIEV 72
           + + G  V  +G+NWGT A+H LPP  +V++ K NGI KVKLFDAD   + AL+G+ I V
Sbjct: 16  LSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVLRALSGSNIGV 75

Query: 73  MVAIPNDQLASM-GDYNRAKQWVQRNVTRYNFDGG--VIIKYVAVGNEPFLSSYNGSFLN 132
            + I N  L S+      A+ WV  NVTRY F+GG  V I+YVAVG EPFL SY   +  
Sbjct: 76  TIGIQNSMLKSLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAVGEEPFLQSYGNQYKP 135

Query: 133 VTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTEIVQ 192
               A  NIQNAL +A L + +K  VP + D + S   S  PS+G FR+D+N  M E++ 
Sbjct: 136 FVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLS--ESGRPSSGHFRADLNKTMIELLS 195

Query: 193 FLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTLV 252
           FL+K+++PF V I PFLS + N N   D++ F   +    D      Y N FD ++DTLV
Sbjct: 196 FLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKD--GRKTYRNSFDLSYDTLV 255

Query: 253 SSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLRSGFIEVY 312
           S+L  +G  ++ I+V ++GWPTDG +NA    A  F+ GL+  L  KK   L    +E Y
Sbjct: 256 SALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHL-EKKTASLPRPPVETY 315

Query: 313 LFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQYLPQKWCM 372
           + S LDE+ ++++ G+FERHWG+F +DGQ K+    + + Q    + AQNVQYLP KWC+
Sbjct: 316 IESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFNHKNQ----VNAQNVQYLPPKWCV 375

Query: 373 FNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMAC 432
            N N KDLS  +     AC  +DCTS+  G SC+ +   GN SYAFN  +Q  +    +C
Sbjct: 376 VN-NNKDLSNASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHSAESC 435

Query: 433 NFQGLATMTSQNLSQGTCNFIIQI-ASSPRSFAASFVSLASMVLLF 475
           NF GL  +T+ + S+  C F IQ+  S   S   +F     ++LLF
Sbjct: 436 NFGGLGLITTVDPSEDNCRFSIQLDTSHSSSQTPNFFQSWPLLLLF 470

[Close]

BLAST of Lag0022145 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 7.7e-59
Identity = 151/436 (34.63%), Postives = 230/436 (52.75%), Query Frame = 0


Query: 23  LGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTMGALAGTGIEVMVAIPNDQLA 82
           LGVN+G ++++    +  V + K   IQKV+LF A+ + + A A TG+E+++   N  + 
Sbjct: 30  LGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGDIP 89

Query: 83  SM-GDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQNA 142
           ++  + N A Q+V+ NV  +     +I   + VGNE  L+S +   ++   PA+QN+QNA
Sbjct: 90  TLASNPNVASQFVKSNVMSFYPASNIIA--ITVGNE-VLTSGDQKLISQLLPAMQNVQNA 149

Query: 143 LNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTEIVQFLSKNNAPFTVN 202
           LN A LG  +K +      V +    S  PS+G F   +   M  ++QF S N+APF ++
Sbjct: 150 LNAASLGGKVKVSTVHAMAVLS---QSYPPSSGVFNPGLGDTMKALLQFQSANDAPFMIS 209

Query: 203 IYPFLSLYGNDNFPFDYAFFDGASNP-VVDIGTGIQYTNVFDANFDTLVSSLKAVGLGDM 262
            YP+ + Y N   P   AF     N   VD G G +YTN+FDA  D + S+L A+G  D+
Sbjct: 210 PYPYFA-YKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDI 269

Query: 263 PILVGEVGWPTDGDKN---ANVGNAYRFYNGLLPRLAAKKGTPLRSG-FIEVYLFSFLDE 322
            I+V E GWP  GD N    ++ NA  +   L+  L +K GTPL  G  I+ YLFS  DE
Sbjct: 270 EIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDE 329

Query: 323 NGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQN-VQYLPQKWCMFNPNAK 382
           + K+ A  S E+++G+F+ DG   + + L    QN                WC+  P   
Sbjct: 330 DKKTGA--SSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPTPTPKAAGSWCVPKPGVS 389

Query: 383 DLSKLADNIDYACTFS-DCTSLGYGSSCNNLD-ANGNASYAFNMYFQVQNQDEMACNFQG 442
           D  +L  NI+YAC    DC  +  G +C   +    +A+Y  N+Y+Q   ++   C+F  
Sbjct: 390 D-DQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQ 449

Query: 443 LATMTSQNLSQGTCNF 450
            AT+T+ N S G CNF
Sbjct: 450 TATLTNTNPSYGACNF 455

[Close]

BLAST of Lag0022145 vs. ExPASy TrEMBL
Match: A0A0A0LFN2 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G843830 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.2e-264
Identity = 449/479 (93.74%), Postives = 463/479 (96.66%), Query Frame = 0


Query: 1   MGRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MG LGFLKWVLLVGVLG CVEGLGVNWGTMANH+LPPKT+VQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGSLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  TMGALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFL 120
           +MGALAGTGIEVMVAIPNDQL++MGDYNRAKQWVQRNVTRY FDGGV IKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180
           SSYNGSFLNVTFPAL NIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALLNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180

Query: 181 NGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LMT+IVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMTDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKN NVGNAYRFYNGLLPRLAA KGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNGNVGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNV 360
           LR GFIEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKF MDLSGQGQNKLL+GAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASFVSLASMVLLFTALLL 480
           VQNQDE+ACNFQGLAT+TSQNLSQGTCNFIIQ+ASSP S AASFVSLASM+LLF ALLL
Sbjct: 421 VQNQDELACNFQGLATVTSQNLSQGTCNFIIQLASSPPSHAASFVSLASMILLFIALLL 479

[Close]

BLAST of Lag0022145 vs. ExPASy TrEMBL
Match: A0A5D3E281 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00130 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 7.7e-264
Identity = 449/479 (93.74%), Postives = 463/479 (96.66%), Query Frame = 0


Query: 1   MGRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRLGFLKWVLLVGVLG CVEGLGVNWGTMANH+LPPKT+VQM KDNGIQKVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQS 60

Query: 61  TMGALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFL 120
           +MGALAGTGIEVMVAIPNDQL++MGDYNRAKQWVQRNVTRY FDGGV IKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LM +IVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA KGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNV 360
           LR GFIEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKF MDLSGQGQNKLL+GAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           +YLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 EYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASFVSLASMVLLFTALLL 480
           VQNQDE+ACNFQGLAT+TS+NLSQGTCNFIIQ+ASSP S AASFVSLASMVLLF ALLL
Sbjct: 421 VQNQDELACNFQGLATITSRNLSQGTCNFIIQLASSPPSHAASFVSLASMVLLFIALLL 479

[Close]

BLAST of Lag0022145 vs. ExPASy TrEMBL
Match: A0A1S3CNJ0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103502975 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 7.7e-264
Identity = 449/479 (93.74%), Postives = 463/479 (96.66%), Query Frame = 0


Query: 1   MGRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRLGFLKWVLLVGVLG CVEGLGVNWGTMANH+LPPKT+VQM KDNGIQKVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQS 60

Query: 61  TMGALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFL 120
           +MGALAGTGIEVMVAIPNDQL++MGDYNRAKQWVQRNVTRY FDGGV IKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LM +IVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA KGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNV 360
           LR GFIEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKF MDLSGQGQNKLL+GAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           +YLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 EYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASFVSLASMVLLFTALLL 480
           VQNQDE+ACNFQGLAT+TS+NLSQGTCNFIIQ+ASSP S AASFVSLASMVLLF ALLL
Sbjct: 421 VQNQDELACNFQGLATITSRNLSQGTCNFIIQLASSPPSHAASFVSLASMVLLFIALLL 479

[Close]

BLAST of Lag0022145 vs. ExPASy TrEMBL
Match: A0A6J1DNQ1 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111022903 PE=3 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 2.3e-263
Identity = 446/478 (93.31%), Postives = 465/478 (97.28%), Query Frame = 0


Query: 3   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTM 62
           RLGFLKWVLLVGVLGCCVEGLGVNWGTMANH+LPPKT+VQMFKDNGI+KVKLFDADQSTM
Sbjct: 4   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTM 63

Query: 63  GALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSS 122
           GALAG+GIEVMVAIPNDQLA+MGDY RA+QWVQRNVTRYNFDGGV+IKYVAVGNEPFLSS
Sbjct: 64  GALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSS 123

Query: 123 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 182
           YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING
Sbjct: 124 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 183

Query: 183 LMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFD 242
           LMT+IVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD G GIQYTNVFD
Sbjct: 184 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFD 243

Query: 243 ANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLR 302
           ANFDTLVSSLKAVG GDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAA KGTPLR
Sbjct: 244 ANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLR 303

Query: 303 SGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQY 362
           +G+IEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKF MDLSGQGQNKLL+GAQ+VQY
Sbjct: 304 AGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 363

Query: 363 LPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 422
           LP+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ
Sbjct: 364 LPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423

Query: 423 NQDEMACNFQGLATMTSQNLSQGTCNFIIQI-ASSPRSFAASFVSLASMVLLFTALLL 480
           NQDEMACNFQGLAT+TSQNLSQGTCNFIIQ+ +SSP   AASFVSLASMV+L TAL L
Sbjct: 424 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALXL 481

[Close]

BLAST of Lag0022145 vs. ExPASy TrEMBL
Match: A0A6J1I9Q3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472848 PE=3 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 6.6e-263
Identity = 443/479 (92.48%), Postives = 460/479 (96.03%), Query Frame = 0


Query: 1   MGRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRL  L WVLLVGVLGCC+EGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILNWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60

Query: 61  TMGALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFL 120
           +MGALAGTGIEVMVAIPNDQL +MGDYNRAKQWVQRNV+RY FDGGV IKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180
           SSYNGSFLNVTFPALQN+QNALNEAG GD+IKATVPLNADVYNSPVN+PYPSAGRFRSDI
Sbjct: 121 SSYNGSFLNVTFPALQNVQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NGLMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           NGLMT+IVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD+ TGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDVNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA KGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRSGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNV 360
           LR GFIEVYLFSFLDENGKSIAPG+FERHWGIF YDGQPKF MDLSGQGQNKLL+GAQ+V
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIASSPRSFAASFVSLASMVLLFTALLL 480
           VQNQDE+ACNFQGLAT++SQNLSQGTC FIIQ+ASS  SF ASFVSL SM LLFTA+LL
Sbjct: 421 VQNQDELACNFQGLATISSQNLSQGTCKFIIQLASSSPSFDASFVSLVSMALLFTAMLL 479

[Close]

BLAST of Lag0022145 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 732.6 bits (1890), Expect = 2.0e-211
Identity = 356/467 (76.23%), Postives = 402/467 (86.08%), Query Frame = 0


Query: 9   WVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTMGALAGT 68
           +V+++G LG  V GLGVNWGTMA HKLPPKT+VQM KDN I KVKLFDAD++TMGALAG+
Sbjct: 11  FVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 70

Query: 69  GIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSSYNGSFL 128
           G+EVMVAIPNDQL  M  Y+RAK WV++NVTRYNFDGGV I +VAVGNEPFL SYNGSF+
Sbjct: 71  GLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFI 130

Query: 129 NVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTEIV 188
           N+TFPAL NIQNALNEAGLG+S+KATVPLNADVY+SP ++P PSAGRFR DI G MT+IV
Sbjct: 131 NLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIV 190

Query: 189 QFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTL 248
            FL KNNAP T+NIYPFLSLYGND+FP +YAFFDGA  P+ D   GI YTNVFDANFDTL
Sbjct: 191 DFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANFDTL 250

Query: 249 VSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLRSGFIEV 308
           VSSLKAVG GDMPI+VGEVGWPT+GDK+AN G+AYRFYNGLLPRL   KGTPLR  +IEV
Sbjct: 251 VSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEV 310

Query: 309 YLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQYLPQKWC 368
           YLF  LDE+ KSIAPG FERHWGIF++DGQPKF +DLSGQGQ+K LIGAQNV YLP KWC
Sbjct: 311 YLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWC 370

Query: 369 MFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMA 428
            FNP AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQDE A
Sbjct: 371 TFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESA 430

Query: 429 CNFQGLATMTSQNLSQGTCNFIIQI-ASSPRSFAASFVSLASMVLLF 475
           C FQGLAT+T+QN+SQG CNF IQI ASS  SF+ S  SL  +++ F
Sbjct: 431 CYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWF 474

[Close]

BLAST of Lag0022145 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 732.6 bits (1890), Expect = 2.0e-211
Identity = 356/467 (76.23%), Postives = 402/467 (86.08%), Query Frame = 0


Query: 9   WVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTMGALAGT 68
           +V+++G LG  V GLGVNWGTMA HKLPPKT+VQM KDN I KVKLFDAD++TMGALAG+
Sbjct: 11  FVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 70

Query: 69  GIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSSYNGSFL 128
           G+EVMVAIPNDQL  M  Y+RAK WV++NVTRYNFDGGV I +VAVGNEPFL SYNGSF+
Sbjct: 71  GLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFI 130

Query: 129 NVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTEIV 188
           N+TFPAL NIQNALNEAGLG+S+KATVPLNADVY+SP ++P PSAGRFR DI G MT+IV
Sbjct: 131 NLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIV 190

Query: 189 QFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTL 248
            FL KNNAP T+NIYPFLSLYGND+FP +YAFFDGA  P+ D   GI YTNVFDANFDTL
Sbjct: 191 DFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANFDTL 250

Query: 249 VSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLRSGFIEV 308
           VSSLKAVG GDMPI+VGEVGWPT+GDK+AN G+AYRFYNGLLPRL   KGTPLR  +IEV
Sbjct: 251 VSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEV 310

Query: 309 YLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQYLPQKWC 368
           YLF  LDE+ KSIAPG FERHWGIF++DGQPKF +DLSGQGQ+K LIGAQNV YLP KWC
Sbjct: 311 YLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWC 370

Query: 369 MFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMA 428
            FNP AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQDE A
Sbjct: 371 TFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESA 430

Query: 429 CNFQGLATMTSQNLSQGTCNFIIQI-ASSPRSFAASFVSLASMVLLF 475
           C FQGLAT+T+QN+SQG CNF IQI ASS  SF+ S  SL  +++ F
Sbjct: 431 CYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWF 474

[Close]

BLAST of Lag0022145 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 713.8 bits (1841), Expect = 9.5e-206
Identity = 350/470 (74.47%), Postives = 401/470 (85.32%), Query Frame = 0


Query: 4   LGFLKWVLLVGVLG-CCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTM 63
           +  L +V+  G++G   V+GLGVNWGTMA HKLPPK +VQM KDN I KVKLFDAD++TM
Sbjct: 1   MNLLAFVVGFGIMGIVMVDGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTM 60

Query: 64  GALAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSS 123
            AL+G+G+EVMVAIPNDQL  MG Y+RAK WV +NVTRYNF+GGV I +VAVGNEPFL S
Sbjct: 61  SALSGSGLEVMVAIPNDQLKVMGSYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKS 120

Query: 124 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 183
           YNGSF+N+TFPALQNIQNALNEAGLG S+KATVPLNADVY+SP ++P PSAGRFR DI G
Sbjct: 121 YNGSFINLTFPALQNIQNALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIG 180

Query: 184 LMTEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFD 243
            MT+IV FL  N+AP T+NIYPFLSLYGND+FP +YAFFDGA  PV D   GI YTNVFD
Sbjct: 181 QMTQIVDFLGNNSAPITINIYPFLSLYGNDDFPLNYAFFDGA-KPVDD--NGIAYTNVFD 240

Query: 244 ANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLR 303
           ANFDTLVS+LKAVG GDMPI+VGEVGWPT+GDK+AN G+AYRFYNGLLPRL   +GTPLR
Sbjct: 241 ANFDTLVSALKAVGHGDMPIIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLR 300

Query: 304 SGFIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQY 363
             +IEVYLF  LDE+ KSIAPG FERHWGIF++DGQPKF +DLSGQGQNKLLIGA+NV Y
Sbjct: 301 PTYIEVYLFGLLDEDAKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTY 360

Query: 364 LPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423
            P+KWCMFN  AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNMYFQV+
Sbjct: 361 QPKKWCMFNTEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVK 420

Query: 424 NQDEMACNFQGLATMTSQNLSQGTCNFIIQI-ASSPRSFAASFVSLASMV 472
           NQDE AC FQGLAT+T++N+SQG CNF IQI AS+  SF++S V L + V
Sbjct: 421 NQDEDACIFQGLATITTKNISQGQCNFPIQIVASTASSFSSSLVLLIAGV 467

[Close]

BLAST of Lag0022145 vs. TAIR 10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 661.0 bits (1704), Expect = 7.3e-190
Identity = 320/461 (69.41%), Postives = 385/461 (83.51%), Query Frame = 0


Query: 20  VEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTMGALAGTGIEVMVAIPND 79
           V GLGVNWGTMA+H+LPPKT+V+M KDN IQKVKLFDAD +TMGALAG+G+EVMVAIPND
Sbjct: 30  VSGLGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNTMGALAGSGVEVMVAIPND 89

Query: 80  QLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQ 139
            L +MG+Y RAK WVQRNV+R+NF+ GV IKYVAVGNEPFL++YNGSF+N+T+PAL NIQ
Sbjct: 90  LLLAMGNYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGSFINLTYPALFNIQ 149

Query: 140 NALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTEIVQFLSKNNAPFT 199
            ALNEAG+GD  KATVPLNADVYNSP ++  PSAGRFRSDI   MT+IV FL++N APFT
Sbjct: 150 TALNEAGVGDFTKATVPLNADVYNSPPDNQVPSAGRFRSDIIQEMTQIVNFLAQNKAPFT 209

Query: 200 VNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTLVSSLKAVGLGD 259
           VNIYPFLSLY + +FPF+YAFFDG  N V D   G+ YTNVFDA+FDTL++SL A+  G+
Sbjct: 210 VNIYPFLSLYLSSDFPFEYAFFDG-QNTVND--NGVIYTNVFDASFDTLLASLNALNHGN 269

Query: 260 MPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLRSGFIEVYLFSFLDENGK 319
           M ++VGEVGWPTDGDKNANV NA RFY+GLLPRLA   GTP+R G+IEVYLF F+DE+ K
Sbjct: 270 MEVIVGEVGWPTDGDKNANVPNAERFYSGLLPRLANNVGTPMRKGYIEVYLFGFIDEDAK 329

Query: 320 SIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQYLPQKWCMFNPNAK-DLS 379
           S+APG+FERHWGIF++DGQPKF +D  GQGQ K L GAQNVQY   +WCMFNPN + ++S
Sbjct: 330 SVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWCMFNPNGRGNMS 389

Query: 380 KLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMACNFQGLATMT 439
           +L DNI+YAC+ SDCT+LGYGSSC NLDANGNASYAFNMYFQVQNQ+  AC+F+GLAT+T
Sbjct: 390 RLGDNINYACSHSDCTALGYGSSCGNLDANGNASYAFNMYFQVQNQEAQACDFEGLATIT 449

Query: 440 SQNLSQGTCNFIIQIAS-SPRSFAASFVSLASMVLLFTALL 479
           +QN+SQG CNF IQI   S   +  S+ S+  + L+ + L+
Sbjct: 450 TQNISQGQCNFPIQIGEPSSGHYDYSYGSMVRLCLVMSGLV 487

[Close]

BLAST of Lag0022145 vs. TAIR 10
Match: AT5G18220.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 654.1 bits (1686), Expect = 8.9e-188
Identity = 327/482 (67.84%), Postives = 385/482 (79.88%), Query Frame = 0


Query: 5   GFLKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSTMGA 64
           G    + +V +L   V  LGVNWGTM+ H+LPPKT+VQM KDN ++KVKLFDAD +TM A
Sbjct: 12  GIFLCITIVSLLSGDVSALGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADTNTMVA 71

Query: 65  LAGTGIEVMVAIPNDQLASMGDYNRAKQWVQRNVTRYNFDGGVIIKYVAVGNEPFLSSYN 124
           LAG+GIEVMVAIPNDQL +MG YNRAK WV+RN+TR+N D  V IKYVAVGNEPFL++YN
Sbjct: 72  LAGSGIEVMVAIPNDQLKAMGSYNRAKDWVRRNITRFNDD--VKIKYVAVGNEPFLTAYN 131

Query: 125 GSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLM 184
           GSF+N+T+PAL NIQ ALNEAG+GD IKATVPLNADVYNSP+ +P PSAG FR DI   M
Sbjct: 132 GSFINLTYPALFNIQKALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIFEEM 191

Query: 185 TEIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDAN 244
             IV FL+ N APFTVNIYPFLSLY + +FPFDYAFF+G  N V D   G+ YTNVFDAN
Sbjct: 192 KLIVNFLAHNKAPFTVNIYPFLSLYLSSDFPFDYAFFNG-QNTVSD--NGVIYTNVFDAN 251

Query: 245 FDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAAKKGTPLRSG 304
           FDTL++SLKA+G GDM ++VGEVGWPTDGDKNAN+ NA RFY GLLP+LAA +GTP+R G
Sbjct: 252 FDTLLASLKALGHGDMTVIVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMRPG 311

Query: 305 FIEVYLFSFLDENGKSIAPGSFERHWGIFQYDGQPKFAMDLSGQGQNKLLIGAQNVQYLP 364
           +IEVYLF F+DE+ KSIAPG+FERHWGIF+YDGQPKF  DLSG GQ K+L GAQNVQYL 
Sbjct: 312 YIEVYLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLR 371

Query: 365 QKWCMFNPNA----KDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 424
            +WCMFNPNA     + ++L DN++YACTFSDCT+LGYGSSC NLD  GNASYAFNMYFQ
Sbjct: 372 NQWCMFNPNALTFSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNMYFQ 431

Query: 425 VQNQDEMACNFQGLATMTSQNLSQGTCNFIIQIAS-----SPRSFAASFVSLASMVLLFT 478
           VQNQ   AC+F+GLA +T++N+S+  CNF IQI       S  +      SL SM+LLFT
Sbjct: 432 VQNQKAEACDFEGLAIITTRNISREQCNFPIQIGDPTSGHSDHNHRFISFSLVSMLLLFT 488

The following BLAST results are available for this feature:

BLAST of Lag0022145 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903901.1	6.9e-267	94.78	glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida]	[more]
XP_004141142.1	2.5e-264	93.74	glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN59764.1 hypothetical pr...	[more]
XP_008465328.1	1.6e-263	93.74	PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo] >KAA0051277.1 ...	[more]
XP_023526667.1	3.6e-263	93.11	glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022155900.1	4.7e-263	93.31	glucan endo-1,3-beta-glucosidase 8-like [Momordica charantia]	[more]

BLAST of Lag0022145 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NKW9	2.8e-210	76.23	Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ...	[more]
Q9M088	2.3e-135	52.82	Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ...	[more]
Q93Z08	4.2e-134	52.42	Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ...	[more]
Q9FGH4	2.3e-95	42.27	Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ...	[more]
Q94G86	7.7e-59	34.63	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]

BLAST of Lag0022145 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFN2	1.2e-264	93.74	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G843830 PE=3...	[more]
A0A5D3E281	7.7e-264	93.74	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CNJ0	7.7e-264	93.74	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103502975 PE=3 SV...	[more]
A0A6J1DNQ1	2.3e-263	93.31	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111022903 ...	[more]
A0A6J1I9Q3	6.6e-263	92.48	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472848 PE=...	[more]

BLAST of Lag0022145 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64760.1	2.0e-211	76.23	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.2	2.0e-211	76.23	O-Glycosyl hydrolases family 17 protein	[more]
AT2G19440.1	9.5e-206	74.47	O-Glycosyl hydrolases family 17 protein	[more]
AT3G04010.1	7.3e-190	69.41	O-Glycosyl hydrolases family 17 protein	[more]
AT5G18220.1	8.9e-188	67.84	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 366..449 e-value: 6.4E-36 score: 135.3
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 366..436 e-value: 7.4E-18 score: 65.0
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 22..352 e-value: 5.6E-110 score: 369.2
None	No IPR available	GENE3D	1.20.58.1040		coord: 363..459 e-value: 9.4E-9 score: 37.5
None	No IPR available	PANTHER	PTHR32227:SF95	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 8-RELATED	coord: 8..457
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 23..344 e-value: 6.1E-66 score: 223.1
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 260..273
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 8..457
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 23..344

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0022145.1	Lag0022145.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds