Lag0022094 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0022094 vs. NCBI nr
Match: XP_004141154.1 (cytochrome P450 87A3 isoform X1 [Cucumis sativus])

HSP 1 Score: 302.0 bits (772), Expect = 3.7e-78
Identity = 140/188 (74.47%), Postives = 164/188 (87.23%), Query Frame = 0

Query: 1   MWLLMGAIFAFSLIMWLYGLKNSKYNGKLPQGSMGFPILGETRQFFAPNSSFDISPFIKE 60
           MW ++GAI +FS+ +W+Y LKN KYNGKLP+GSMGFP +GE+ QFFAPN SFD+SPFIK 
Sbjct: 1   MWFIIGAILSFSVTIWVYALKNPKYNGKLPKGSMGFPFVGESTQFFAPNPSFDLSPFIKH 60

Query: 61  RILKYGPVFKTNLVGKPIIISTDPELNYFIFQREEELFECWYPETFRKIFGSQSVGSVHG 120
           RILKYGP+FKT LVGKP+IIS D ELN+ IFQ+EEELFECWYPETFRKIFG +SVGS+HG
Sbjct: 61  RILKYGPIFKTRLVGKPLIISADAELNHIIFQKEEELFECWYPETFRKIFGVKSVGSLHG 120

Query: 121 FMHKYLKNMIINLFGIESLKGMIFEVEAAATARLRRWASYN-TVELKDEITDMIFDLTAK 180
           FMHKYLKNMI N+FGIESLK MI EVE  +T RL++WAS+N  VELKDEI +MIFDL+AK
Sbjct: 121 FMHKYLKNMITNVFGIESLKNMISEVEVTSTTRLKKWASHNDIVELKDEIANMIFDLSAK 180

Query: 181 KLIGYDPE 188
           +LI YDPE
Sbjct: 181 RLISYDPE 188

BLAST of Lag0022094 vs. NCBI nr
Match: XP_031737523.1 (cytochrome P450 87A3 isoform X2 [Cucumis sativus])

HSP 1 Score: 302.0 bits (772), Expect = 3.7e-78
Identity = 140/188 (74.47%), Postives = 164/188 (87.23%), Query Frame = 0

Query: 1   MWLLMGAIFAFSLIMWLYGLKNSKYNGKLPQGSMGFPILGETRQFFAPNSSFDISPFIKE 60
           MW ++GAI +FS+ +W+Y LKN KYNGKLP+GSMGFP +GE+ QFFAPN SFD+SPFIK 
Sbjct: 1   MWFIIGAILSFSVTIWVYALKNPKYNGKLPKGSMGFPFVGESTQFFAPNPSFDLSPFIKH 60

Query: 61  RILKYGPVFKTNLVGKPIIISTDPELNYFIFQREEELFECWYPETFRKIFGSQSVGSVHG 120
           RILKYGP+FKT LVGKP+IIS D ELN+ IFQ+EEELFECWYPETFRKIFG +SVGS+HG
Sbjct: 61  RILKYGPIFKTRLVGKPLIISADAELNHIIFQKEEELFECWYPETFRKIFGVKSVGSLHG 120

Query: 121 FMHKYLKNMIINLFGIESLKGMIFEVEAAATARLRRWASYN-TVELKDEITDMIFDLTAK 180
           FMHKYLKNMI N+FGIESLK MI EVE  +T RL++WAS+N  VELKDEI +MIFDL+AK
Sbjct: 121 FMHKYLKNMITNVFGIESLKNMISEVEVTSTTRLKKWASHNDIVELKDEIANMIFDLSAK 180

Query: 181 KLIGYDPE 188
           +LI YDPE
Sbjct: 181 RLISYDPE 188

BLAST of Lag0022094 vs. NCBI nr
Match: XP_022983296.1 (cytochrome P450 87A3-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 300.4 bits (768), Expect = 1.1e-77
Identity = 140/188 (74.47%), Postives = 157/188 (83.51%), Query Frame = 0

Query: 1   MWLLMGAIFAFSLIMWLYGLKNSKYNGKLPQGSMGFPILGETRQFFAPNSSFDISPFIKE 60
           MW+LM  IF  S+I  L+ LK SKYN KLP+GSMGFPILGET  FFAPN SFD+SPFIK 
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RILKYGPVFKTNLVGKPIIISTDPELNYFIFQREEELFECWYPETFRKIFGSQSVGSVHG 120
           R+ KYGP+FKT+LVGKP+IISTDP+LNY IF++EE LFECWYPETF+KIFG Q  GS+HG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIINLFGIESLKGMIFEVEAAATARLRRWASYNTVELKDEITDMIFDLTAKK 180
           FMHKYLKNMI+N FGIE LK M+FEVEAAA  RLRRWAS   VELKDEI +MI DLTA K
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LIGYDPEN 189
           LI YDPEN
Sbjct: 181 LISYDPEN 188

BLAST of Lag0022094 vs. NCBI nr
Match: XP_022983295.1 (cytochrome P450 87A3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 300.4 bits (768), Expect = 1.1e-77
Identity = 140/188 (74.47%), Postives = 157/188 (83.51%), Query Frame = 0

Query: 1   MWLLMGAIFAFSLIMWLYGLKNSKYNGKLPQGSMGFPILGETRQFFAPNSSFDISPFIKE 60
           MW+LM  IF  S+I  L+ LK SKYN KLP+GSMGFPILGET  FFAPN SFD+SPFIK 
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RILKYGPVFKTNLVGKPIIISTDPELNYFIFQREEELFECWYPETFRKIFGSQSVGSVHG 120
           R+ KYGP+FKT+LVGKP+IISTDP+LNY IF++EE LFECWYPETF+KIFG Q  GS+HG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIINLFGIESLKGMIFEVEAAATARLRRWASYNTVELKDEITDMIFDLTAKK 180
           FMHKYLKNMI+N FGIE LK M+FEVEAAA  RLRRWAS   VELKDEI +MI DLTA K
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LIGYDPEN 189
           LI YDPEN
Sbjct: 181 LISYDPEN 188

BLAST of Lag0022094 vs. NCBI nr
Match: XP_022983294.1 (cytochrome P450 87A3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 300.4 bits (768), Expect = 1.1e-77
Identity = 140/188 (74.47%), Postives = 157/188 (83.51%), Query Frame = 0

Query: 1   MWLLMGAIFAFSLIMWLYGLKNSKYNGKLPQGSMGFPILGETRQFFAPNSSFDISPFIKE 60
           MW+LM  IF  S+I  L+ LK SKYN KLP+GSMGFPILGET  FFAPN SFD+SPFIK 
Sbjct: 1   MWVLMAIIFGMSIITCLHALKYSKYNSKLPKGSMGFPILGETNHFFAPNPSFDVSPFIKH 60

Query: 61  RILKYGPVFKTNLVGKPIIISTDPELNYFIFQREEELFECWYPETFRKIFGSQSVGSVHG 120
           R+ KYGP+FKT+LVGKP+IISTDP+LNY IF++EE LFECWYPETF+KIFG Q  GS+HG
Sbjct: 61  RVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQEETLFECWYPETFKKIFGDQFFGSLHG 120

Query: 121 FMHKYLKNMIINLFGIESLKGMIFEVEAAATARLRRWASYNTVELKDEITDMIFDLTAKK 180
           FMHKYLKNMI+N FGIE LK M+FEVEAAA  RLRRWAS   VELKDEI +MI DLTA K
Sbjct: 121 FMHKYLKNMIVNAFGIECLKSMVFEVEAAAITRLRRWASCEVVELKDEIANMILDLTANK 180

Query: 181 LIGYDPEN 189
           LI YDPEN
Sbjct: 181 LISYDPEN 188

BLAST of Lag0022094 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 228.8 bits (582), Expect = 5.2e-59
Identity = 103/192 (53.65%), Postives = 146/192 (76.04%), Query Frame = 0

Query: 3   LLMGAIFA-FSLIMWLYGLKNSKYNGKLPQGSMGFPILGETRQFFAPNSSFDISPFIKER 62
           LL  A+ A  +L+ W Y   + + NG+LP GS+G P++GET QFFAPN + D+SPF+KER
Sbjct: 44  LLCAALAAVVALLRWAYRWSHPRSNGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKER 103

Query: 63  ILKYGPVFKTNLVGKPIIISTDPELNYFIFQREEELFECWYPETFRKIFGSQSVGSVHGF 122
           I +YG +FKT++VG+P+++S DPE+NY++FQ+E +LFE WYP+TF +IFG  +VGS+HGF
Sbjct: 104 IKRYGSIFKTSVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGRDNVGSLHGF 163

Query: 123 MHKYLKNMIINLFGIESLKGMIF-EVEAAATARLRRWASYNTVELKDEITDMIFDLTAKK 182
           M+KYLK +++ L+G E+LK ++  E +AA    L  WAS  +VELK+ I+ MIFDLTAKK
Sbjct: 164 MYKYLKTLVLRLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIFDLTAKK 223

Query: 183 LIGYDPENRQKI 193
           LIGYDP    ++
Sbjct: 224 LIGYDPSKPSQV 235

BLAST of Lag0022094 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 146.7 bits (369), Expect = 2.6e-34
Identity = 75/191 (39.27%), Postives = 118/191 (61.78%), Query Frame = 0

Query: 1   MWLLMGAIFAFSLIMWLYGLKNSKYNGKLPQGSMGFPILGETRQFFAPNSSFDISPFIKE 60
           +W++  A+ A  +  WLY   N K NGKLP GSMG PI+GET  FF P+  ++ISPF+K+
Sbjct: 9   VWVI--AVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPFVKK 68

Query: 61  RILKYGPVFKTNLVGKPIIISTDPELNYFIFQREEELFECWYPETFRKIFGSQSVGSVHG 120
           R+LKYGP+F+TN+ G   ++ T+P++ + +F++E + F   YPE F K FG ++V   HG
Sbjct: 69  RMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFLKHG 128

Query: 121 FMHKYLKNMIINLFGIESL-KGMIFEVEAAATARLRRWASYNTVELKDEITDMIF-DLTA 180
            +HK++K + +   G E+L K MI E++      LR  A+  + + K+ +  +I   LT 
Sbjct: 129 NIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTP 188

Query: 181 KKLIGYDPENR 190
           K +    PE +
Sbjct: 189 KIISNLKPETQ 197

BLAST of Lag0022094 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 4.9e-33
Identity = 69/190 (36.32%), Postives = 113/190 (59.47%), Query Frame = 0

Query: 1   MW---LLMGAIFAFSLIMWLYGLKNSKYNGKLPQGSMGFPILGETRQFFAPNSSFDISPF 60
           MW   L +  +F    I W+   ++SK+NG LP G+MG P++GET Q   P+ S D+ PF
Sbjct: 1   MWTVVLGLATLFVAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IKERILKYGPVFKTNLVGKPIIISTDPELNYFIFQREEELFECWYPETFRKIFGSQSVG- 120
           I++++ +YGP+FKT L G+P+++S D E N +I  +E    E WY +T  K FG  +   
Sbjct: 61  IQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 SVHGFMHKYLKNMIINLFGIESLKGMIFE-VEAAATARLRRWASYNTVELKDEITDMIFD 180
              G +HKY++++ +N FG E+L+      +EA++   L  W++  +VE+K+    M+F 
Sbjct: 121 KALGLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVFR 180

Query: 181 LTAKKLIGYD 186
            +  K+ G D
Sbjct: 181 TSVNKMFGED 190

BLAST of Lag0022094 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.9e-25
Identity = 64/186 (34.41%), Postives = 105/186 (56.45%), Query Frame = 0

Query: 3   LLMGAIFAFSLIMWLYGLKNSKYNGK-LPQGSMGFPILGETRQFFAPNSSFDISPFIKER 62
           LL+ +I A  L++    L+ ++Y    LP GS+G P++GET Q      + +  PFI ER
Sbjct: 8   LLLSSIAAGFLLL----LRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDER 67

Query: 63  ILKYGPVFKTNLVGKPIIISTDPELNYFIFQREEELFECWYPETFRKIFGSQSVGSVHGF 122
           + +YG VF T+L G+P I S DPE N F+ Q E +LFEC YP +   + G  S+  + G 
Sbjct: 68  VARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGS 127

Query: 123 MHKYLKNMIINLFGIESLKG-MIFEVEAAATARLRRWASYNTVELKDEITDMIFDLTAKK 182
           +HK + ++ ++      +K  ++ +++      L  W+S   V L +E   + F+LT K+
Sbjct: 128 LHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSS--RVLLMEEAKKITFELTVKQ 187

Query: 183 LIGYDP 187
           L+ +DP
Sbjct: 188 LMSFDP 187

BLAST of Lag0022094 vs. ExPASy Swiss-Prot
Match: Q6F4F5 (Cytochrome P450 724B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP724B1 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 7.1e-24
Identity = 60/170 (35.29%), Postives = 91/170 (53.53%), Query Frame = 0

Query: 26  NGKLPQGSMGFPILGETRQFFAPNSSFDISPFIKERILKYGPVFKTNLVGKPIIISTDPE 85
           N K P+GS G+P+LGET +F +P++S  +  F+++   +YG VFK++L   P I+S D E
Sbjct: 32  NPKAPKGSFGWPLLGETLRFLSPHASNTLGSFLEDHCSRYGRVFKSHLFCTPTIVSCDQE 91

Query: 86  LNYFIFQREEELFECWYPETFRKIFGSQSVGSVHGFMHKYLKNMIINLFGIESLK-GMIF 145
           LN+FI Q EE LF+C YP     I G  S+  V G  HK L+N+ + L     LK   + 
Sbjct: 92  LNHFILQNEERLFQCSYPRPIHGILGKSSMLVVLGEDHKRLRNLALALVTSTKLKPSYLG 151

Query: 146 EVEAAATARLRRW-------ASYNTVELKDEITDMIFDLTAKKLIGYDPE 188
           ++E  A   +  W          N +   +E     F +  K+++G  PE
Sbjct: 152 DIEKIALHIVGSWHGKSKDKGMVNVIAFCEEARKFAFSVIVKQVLGLSPE 201