Lag0020991 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0020991
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DNA-directed RNA polymerase III subunit rpc6-like
Location	chr7: 3781689 .. 3783100 (+)
RNA-Seq Expression	Lag0020991
Synteny	Lag0020991

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACCCATCAATACGGCCCAATACCCTAAAAGCCCATTCCGCTTTCTTTCATTTATGGCAGTTGGCAGAAAATCAAAAAGAACTCCTTAAGATTTTACCTCCTCCTCCCTCTCGCTCCCTCTCTTTAACGACGCCCCTCCGCCTCCTTGCGCCGCCTTCACCGTCGCCCCTCCGCCTCCCGCCGCCGTCGCGTTCCTCTGACAAGGTAATAATCTTACAAGAAAATTCAAATTTTCAGCACAGCCTAATAAAGTTCAAATTGATGGAATCGAACCGAAATTATCGAGCCGATTGGTTCGGTTGAGAATCAGTTTTGCTCGACAATGGACATTTGTTCGATTTTCATTCCTTCGAAATCTAGATCATCCGTTCCATTCCTAATTTAAGAAGAATTTTCTCGAACCGATGTTCAACCACACCCTTTTTCTCATGGTAGTTCAGAGAAAATTTCTACCATATAAAAAGTAACATTTGAGAATTTTTGGAACTAATTTCATTAGTTCTTTGCCATATAGTGATATAAATGTGTGAAATAGAATAATGTATGAATTGATGATGTCTCAAGATCTGAATGGATGGAGTCGATACTATCGTAGTCGTAGTACTAGAAATTTTATCGAAACTTTGAATTTGATCATAACATTATACTTGCAGTGATCTTATTACGTTGTATATAATATATATCATTTTATCCTGTAGAAAATGAGCAGATCACAAGGGCCTTCATCACTGAAAAGAAAAAGGCCAGAAGGAGGAGCTCTTATTGAGCATGAAAAAATGCTCTATGATCTGATTTGCAGCAAGCAAAGCATGGGGATGTGCACATTCGACATGAAAAGAGAGACAAATTTTGCTGAAAGTACAGTAACTAAATCACTCAAGGCCCTTCAAGCTAAAGGCCTGGTAAAAGTGGTTCAGCACTATCAGAACAAAGGGAAGAAATTCTACATGTCGAAAGATTTCGAACCATCGAAAGCGCTGACCGGAGGAGATTGGTACCGGGACGGGATTCTGGACAAGGAATATATCAACACACTGAAAGGGGTTTGTGCAAGAATGATACAGAAGAAGAAGATGATCACCATTGAAGGAATTGTGGAGTCTTTCAAGAGGAGTGGAGCTTCTAAAGTGGAGTTGTCCAAGGAGCAGATTCAAGAGATTGTGAATGCTTTGGTGTTGGACCATGAAGTAATGGAAGTGAAGAGCAATGGAACAGGAGAATTTGATTTTATGCCTGTTGGGAAGATTTGTTATAAATTTTGTTCTAAAGGAGGAGTTAAAGGGGAGCCCAAAACAGGGGCAATGGCTTCTATTCCTTGTGCAGTTTGTCCACAGATTAGTTATTGTACACCAAATGGCATCATTTCGCCCAAGACTTGTGTGTATTACACCAAGTGGTTAGATTTTTGA

mRNA sequence

ATGTACCCATCAATACGGCCCAATACCCTAAAAGCCCATTCCGCTTTCTTTCATTTATGGCAGTTGGCAGAAAATCAAAAAGAACTCCTTAAGATTTTACCTCCTCCTCCCTCTCGCTCCCTCTCTTTAACGACGCCCCTCCGCCTCCTTGCGCCGCCTTCACCGTCGCCCCTCCGCCTCCCGCCGCCGTCGCGTTCCTCTGACAAGAAAATGAGCAGATCACAAGGGCCTTCATCACTGAAAAGAAAAAGGCCAGAAGGAGGAGCTCTTATTGAGCATGAAAAAATGCTCTATGATCTGATTTGCAGCAAGCAAAGCATGGGGATGTGCACATTCGACATGAAAAGAGAGACAAATTTTGCTGAAAGTACAGTAACTAAATCACTCAAGGCCCTTCAAGCTAAAGGCCTGGTAAAAGTGGTTCAGCACTATCAGAACAAAGGGAAGAAATTCTACATGTCGAAAGATTTCGAACCATCGAAAGCGCTGACCGGAGGAGATTGGTACCGGGACGGGATTCTGGACAAGGAATATATCAACACACTGAAAGGGGTTTGTGCAAGAATGATACAGAAGAAGAAGATGATCACCATTGAAGGAATTGTGGAGTCTTTCAAGAGGAGTGGAGCTTCTAAAGTGGAGTTGTCCAAGGAGCAGATTCAAGAGATTGTGAATGCTTTGGTGTTGGACCATGAAGTAATGGAAGTGAAGAGCAATGGAACAGGAGAATTTGATTTTATGCCTGTTGGGAAGATTTGTTATAAATTTTGTTCTAAAGGAGGAGTTAAAGGGGAGCCCAAAACAGGGGCAATGGCTTCTATTCCTTGTGCAGTTTGTCCACAGATTAGTTATTGTACACCAAATGGCATCATTTCGCCCAAGACTTGTGTGTATTACACCAAGTGGTTAGATTTTTGA

Coding sequence (CDS)

ATGTACCCATCAATACGGCCCAATACCCTAAAAGCCCATTCCGCTTTCTTTCATTTATGGCAGTTGGCAGAAAATCAAAAAGAACTCCTTAAGATTTTACCTCCTCCTCCCTCTCGCTCCCTCTCTTTAACGACGCCCCTCCGCCTCCTTGCGCCGCCTTCACCGTCGCCCCTCCGCCTCCCGCCGCCGTCGCGTTCCTCTGACAAGAAAATGAGCAGATCACAAGGGCCTTCATCACTGAAAAGAAAAAGGCCAGAAGGAGGAGCTCTTATTGAGCATGAAAAAATGCTCTATGATCTGATTTGCAGCAAGCAAAGCATGGGGATGTGCACATTCGACATGAAAAGAGAGACAAATTTTGCTGAAAGTACAGTAACTAAATCACTCAAGGCCCTTCAAGCTAAAGGCCTGGTAAAAGTGGTTCAGCACTATCAGAACAAAGGGAAGAAATTCTACATGTCGAAAGATTTCGAACCATCGAAAGCGCTGACCGGAGGAGATTGGTACCGGGACGGGATTCTGGACAAGGAATATATCAACACACTGAAAGGGGTTTGTGCAAGAATGATACAGAAGAAGAAGATGATCACCATTGAAGGAATTGTGGAGTCTTTCAAGAGGAGTGGAGCTTCTAAAGTGGAGTTGTCCAAGGAGCAGATTCAAGAGATTGTGAATGCTTTGGTGTTGGACCATGAAGTAATGGAAGTGAAGAGCAATGGAACAGGAGAATTTGATTTTATGCCTGTTGGGAAGATTTGTTATAAATTTTGTTCTAAAGGAGGAGTTAAAGGGGAGCCCAAAACAGGGGCAATGGCTTCTATTCCTTGTGCAGTTTGTCCACAGATTAGTTATTGTACACCAAATGGCATCATTTCGCCCAAGACTTGTGTGTATTACACCAAGTGGTTAGATTTTTGA

Protein sequence

MYPSIRPNTLKAHSAFFHLWQLAENQKELLKILPPPPSRSLSLTTPLRLLAPPSPSPLRLPPPSRSSDKKMSRSQGPSSLKRKRPEGGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAESTVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLKGVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGEFDFMPVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWLDF

Homology

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BLAST of Lag0020991 vs. NCBI nr
Match: TYK19400.1 (DNA-directed RNA polymerase III subunit rpc6-like [Cucumis melo var. makuwa])

HSP 1 Score: 397.9 bits (1021), Expect = 7.9e-107
Identity = 192/243 (79.01%), Postives = 221/243 (90.95%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE-------GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAES 130
           MS+SQGPSSLKRKRP+       GGALI  EK+LYDLICSKQSMG+CTFDMKRET FAES
Sbjct: 5   MSKSQGPSSLKRKRPDLGTKNSSGGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 64

Query: 131 TVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLK 190
            VTKSLK+LQ KGL+K+VQHYQNKGKKFY+S DFEP+K LTGG+WYRDG LDK+YINTLK
Sbjct: 65  VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 124

Query: 191 GVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGE 250
            VCARMIQ+KK++TIEGIVESFK+SGA KVELS+EQI+EIVNALVLD++VMEVKS+G GE
Sbjct: 125 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELSREQIEEIVNALVLDNQVMEVKSSGFGE 184

Query: 251 FDFMPVGKICYKFCSK-GGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKW 306
           F+F+P+GKICYK+C++ GG+KGEPK GAMASIPC VCP+I+YCTPNGIISP TCVYYTKW
Sbjct: 185 FEFIPIGKICYKYCTQGGGLKGEPKIGAMASIPCGVCPRITYCTPNGIISPATCVYYTKW 244

[Close]

BLAST of Lag0020991 vs. NCBI nr
Match: XP_008448982.1 (PREDICTED: DNA-directed RNA polymerase III subunit rpc6-like [Cucumis melo] >XP_008448983.1 PREDICTED: DNA-directed RNA polymerase III subunit rpc6-like [Cucumis melo])

HSP 1 Score: 397.9 bits (1021), Expect = 7.9e-107
Identity = 192/243 (79.01%), Postives = 221/243 (90.95%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE-------GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAES 130
           MS+SQGPSSLKRKRP+       GGALI  EK+LYDLICSKQSMG+CTFDMKRET FAES
Sbjct: 1   MSKSQGPSSLKRKRPDLGTKNSSGGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 60

Query: 131 TVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLK 190
            VTKSLK+LQ KGL+K+VQHYQNKGKKFY+S DFEP+K LTGG+WYRDG LDK+YINTLK
Sbjct: 61  VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 120

Query: 191 GVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGE 250
            VCARMIQ+KK++TIEGIVESFK+SGA KVELS+EQI+EIVNALVLD++VMEVKS+G GE
Sbjct: 121 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELSREQIEEIVNALVLDNQVMEVKSSGFGE 180

Query: 251 FDFMPVGKICYKFCSK-GGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKW 306
           F+F+P+GKICYK+C++ GG+KGEPK GAMASIPC VCP+I+YCTPNGIISP TCVYYTKW
Sbjct: 181 FEFIPIGKICYKYCTQGGGLKGEPKIGAMASIPCGVCPRITYCTPNGIISPATCVYYTKW 240

[Close]

BLAST of Lag0020991 vs. NCBI nr
Match: TYK13079.1 (DNA-directed RNA polymerase III subunit RPC6-like [Cucumis melo var. makuwa])

HSP 1 Score: 397.1 bits (1019), Expect = 1.3e-106
Identity = 190/242 (78.51%), Postives = 220/242 (90.91%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE-------GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAES 130
           MS+SQGPSSLKRKRP+        GALI  EK+LYDLICSKQSMG+CTFDMKRET FAES
Sbjct: 117 MSKSQGPSSLKRKRPDLSTKNSSVGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 176

Query: 131 TVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLK 190
            VTKSLK+LQ KGL+K+VQHYQNKGKKFY+S DFEP+K LTGG+WYRDG LDK+YINTLK
Sbjct: 177 VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 236

Query: 191 GVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGE 250
            VCARMIQ+KK++TIEGIVESFK+SGA KVEL++EQI+EIVNALVLD++V EVKS+G GE
Sbjct: 237 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELTREQIEEIVNALVLDNQVTEVKSSGFGE 296

Query: 251 FDFMPVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWL 306
           F+F+P+GKICYK+CS+GG+KGEPKTGAMASIPC VCP+I++CTPNGIISP TCVYYTKWL
Sbjct: 297 FEFIPIGKICYKYCSQGGLKGEPKTGAMASIPCGVCPRITHCTPNGIISPATCVYYTKWL 356

[Close]

BLAST of Lag0020991 vs. NCBI nr
Match: XP_008439941.1 (PREDICTED: DNA-directed RNA polymerase III subunit RPC6-like [Cucumis melo])

HSP 1 Score: 397.1 bits (1019), Expect = 1.3e-106
Identity = 190/242 (78.51%), Postives = 220/242 (90.91%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE-------GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAES 130
           MS+SQGPSSLKRKRP+        GALI  EK+LYDLICSKQSMG+CTFDMKRET FAES
Sbjct: 1   MSKSQGPSSLKRKRPDLSTKNSSVGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 60

Query: 131 TVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLK 190
            VTKSLK+LQ KGL+K+VQHYQNKGKKFY+S DFEP+K LTGG+WYRDG LDK+YINTLK
Sbjct: 61  VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 120

Query: 191 GVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGE 250
            VCARMIQ+KK++TIEGIVESFK+SGA KVEL++EQI+EIVNALVLD++V EVKS+G GE
Sbjct: 121 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELTREQIEEIVNALVLDNQVTEVKSSGFGE 180

Query: 251 FDFMPVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWL 306
           F+F+P+GKICYK+CS+GG+KGEPKTGAMASIPC VCP+I++CTPNGIISP TCVYYTKWL
Sbjct: 181 FEFIPIGKICYKYCSQGGLKGEPKTGAMASIPCGVCPRITHCTPNGIISPATCVYYTKWL 240

[Close]

BLAST of Lag0020991 vs. NCBI nr
Match: XP_022149571.1 (DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia] >XP_022149572.1 DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia])

HSP 1 Score: 397.1 bits (1019), Expect = 1.3e-106
Identity = 191/238 (80.25%), Postives = 219/238 (92.02%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE---GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAESTVTK 130
           MSRSQGPSSLKRKRP+   GGALIEHEK+LYDLICSKQSMG+   D+KRET +A+S VTK
Sbjct: 1   MSRSQGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTK 60

Query: 131 SLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLKGVCA 190
           SLKALQAKG++K+V+HYQNKGKKFY+SKDFEPSK LTGG+WY +G LD+EYINTLKG C 
Sbjct: 61  SLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCG 120

Query: 191 RMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGEFDFM 250
           RMIQK K+ TIEGIV+SFK+SGASKV+LS+EQIQEIVNALVLD+EVMEVKS G+GEFDF+
Sbjct: 121 RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFI 180

Query: 251 PVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWLDF 306
           P+GKICYK  +KGGVKGEPKTGAMASIPC VCP+IS+CTPNG+ISP+TCVYYTKWLDF
Sbjct: 181 PIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 238

[Close]

BLAST of Lag0020991 vs. ExPASy Swiss-Prot
Match: Q86JM3 (DNA-directed RNA polymerase III subunit rpc6 OS=Dictyostelium discoideum OX=44689 GN=polr3f PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 9.6e-23
Identity = 69/240 (28.75%), Postives = 116/240 (48.33%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPEGGA----LIEHEKMLYDLICSKQSMGMCTFDMKRETNFAESTVT 130
           +  S   +S K   PE  A    L   + ++Y LI +  + G  T ++K  + + +  +T
Sbjct: 52  IQNSDETTSYKEVNPEDQAKFRGLGVEDFLIYQLIEASSNTGAWTRELKNSSGYQQVQIT 111

Query: 131 KSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLKGVC 190
           K LK L+++ L+K V+  Q+  KK YM  + EPS+ +TGG  Y D   D +YI  +K   
Sbjct: 112 KILKTLESRKLIKSVKSIQSGRKKVYMLYNMEPSREITGGQLYSDQSYDHQYIQIMKMHI 171

Query: 191 ARMIQKKKMITIEGIVESFKR--SGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGEF 250
              +  K  + +  I+   ++    A+   L  E IQ +VN ++ D ++ E++    G  
Sbjct: 172 KTFVDNKGAVDLADIITYLRKVAEEATSQSLGPEDIQALVNTVIYDGDIEEMRDTRMGAM 231

Query: 251 DFMPVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWLD 305
                G I YK       K         ++PC  CP    C+ NG++SPK CVY+ ++L+
Sbjct: 232 LGRRSG-ILYK-----PTKTPIPVNNFCNMPCGNCPVFDICSDNGLVSPKRCVYFKQYLE 285

[Close]

BLAST of Lag0020991 vs. ExPASy Swiss-Prot
Match: Q921X6 (DNA-directed RNA polymerase III subunit RPC6 OS=Mus musculus OX=10090 GN=Polr3f PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.6e-22
Identity = 72/238 (30.25%), Postives = 113/238 (47.48%), Query Frame = 0


Query: 94  EKMLYDLICSKQSMGMCTFDMKRETNFAESTVTKSLKALQAKGLVKVVQHYQNKGKKFYM 153
           EK++Y +I    + G+ + D++ ++N   + + K LK L++K L+K V+      KK YM
Sbjct: 92  EKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYM 151

Query: 154 SKDFEPSKALTGGDWYRDGILDKEYINTLKGVCARMIQKKKMITIEG----IVE---SFK 213
             + +P +++TGG WY D   + E++  L   C + +Q K     E     +++   SF 
Sbjct: 152 LYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPVIQRNSSFA 211

Query: 214 RS----------GASKVELSKEQIQEIVNALVLDHEV----MEVKSNGTGEFD-----FM 273
            S          G SKVELS E I+ I+N L+ D +V    +  K    G  D     + 
Sbjct: 212 SSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYR 271

Query: 274 PVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWLDF 306
            V  I             P TG + + PC +CP    C   G ISP  C+Y T+WL+F
Sbjct: 272 AVNPIL------------PPTGVVRA-PCGLCPVFEDCHEGGEISPSNCIYMTEWLEF 316

[Close]

BLAST of Lag0020991 vs. ExPASy Swiss-Prot
Match: Q2T9S3 (DNA-directed RNA polymerase III subunit RPC6 OS=Bos taurus OX=9913 GN=POLR3F PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 8.1e-22
Identity = 72/238 (30.25%), Postives = 109/238 (45.80%), Query Frame = 0


Query: 94  EKMLYDLICSKQSMGMCTFDMKRETNFAESTVTKSLKALQAKGLVKVVQHYQNKGKKFYM 153
           EK++Y +I    + G+ + D++ ++N   + + K LK L++K L+K V+      KK YM
Sbjct: 92  EKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYM 151

Query: 154 SKDFEPSKALTGGDWYRDGILDKEYINTLKGVCARMIQKKKMITIEGIV-------ESFK 213
             + +P +++TGG WY D   + E++  L   C + +Q K     E           SF 
Sbjct: 152 LYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQNKAETARESKQNPMIQRNSSFA 211

Query: 214 RS----------GASKVELSKEQIQEIVNALVLDHEV----MEVKSNGTGEFD-----FM 273
            S          G SKVELS E I+ I+N L+ D +V    +  K    G  D     + 
Sbjct: 212 SSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYR 271

Query: 274 PVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWLDF 306
            V  I             P TG +   PC +CP    C   G ISP  C+Y T+WL+F
Sbjct: 272 AVSPII------------PPTG-LVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF 316

[Close]

BLAST of Lag0020991 vs. ExPASy Swiss-Prot
Match: Q9H1D9 (DNA-directed RNA polymerase III subunit RPC6 OS=Homo sapiens OX=9606 GN=POLR3F PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 8.1e-22
Identity = 72/238 (30.25%), Postives = 109/238 (45.80%), Query Frame = 0


Query: 94  EKMLYDLICSKQSMGMCTFDMKRETNFAESTVTKSLKALQAKGLVKVVQHYQNKGKKFYM 153
           EK++Y +I    + G+ + D++ ++N   + + K LK L++K L+K V+      KK YM
Sbjct: 92  EKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYM 151

Query: 154 SKDFEPSKALTGGDWYRDGILDKEYINTLKGVCARMIQKKKMITIEGIV-------ESFK 213
             + +P +++TGG WY D   + E++  L   C + +Q K     E           SF 
Sbjct: 152 LYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFA 211

Query: 214 RS----------GASKVELSKEQIQEIVNALVLDHEV----MEVKSNGTGEFD-----FM 273
            S          G SKVELS E I+ I+N L+ D +V    +  K    G  D     + 
Sbjct: 212 SSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYR 271

Query: 274 PVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWLDF 306
            V  I             P TG +   PC +CP    C   G ISP  C+Y T+WL+F
Sbjct: 272 AVNPII------------PPTG-LVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF 316

[Close]

BLAST of Lag0020991 vs. ExPASy Swiss-Prot
Match: P91529 (Probable DNA-directed RNA polymerase III subunit RPC6 OS=Caenorhabditis elegans OX=6239 GN=W09C3.4 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-16
Identity = 69/248 (27.82%), Postives = 113/248 (45.56%), Query Frame = 0


Query: 67  SDKKMSRSQGPSS---LKRKRPEGGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAES 126
           S KK++  Q P     LK    E  A  E E+++Y LI   ++ G+   +++  +   + 
Sbjct: 55  SSKKLAIQQAPGGVLRLKVNTSEQIAGTEEEQVIYSLIEESKTRGIWIKELREGSGLNQL 114

Query: 127 TVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLK 186
            + K+LK+L+ K L+K ++      +K Y+    EP  +LTGG +Y D  LD E INTL 
Sbjct: 115 QLRKTLKSLETKKLIKTIKAV-GTTRKCYILFSIEPDMSLTGGTFYSDQQLDSELINTLI 174

Query: 187 GVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLD--------HEVME 246
            VC      ++   I+    + +    +     +E  Q I    VL+          ++E
Sbjct: 175 SVCGSYAASRRKHAIDENPNNIQMQREASYIRPQEIAQFITEKRVLNVPLSLEDLERILE 234

Query: 247 VKSNGTGEFDFMPVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKT 304
           V +   G  +    GKI  + C             + S+PCA+CP +  C P  +ISP++
Sbjct: 235 V-AVLDGTIERRADGKI--RACPP-----RSSISPLVSVPCAICPVVEDCRPGYVISPQS 293

[Close]

BLAST of Lag0020991 vs. ExPASy TrEMBL
Match: A0A1S3BL06 (DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo OX=3656 GN=LOC103490992 PE=3 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 3.8e-107
Identity = 192/243 (79.01%), Postives = 221/243 (90.95%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE-------GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAES 130
           MS+SQGPSSLKRKRP+       GGALI  EK+LYDLICSKQSMG+CTFDMKRET FAES
Sbjct: 1   MSKSQGPSSLKRKRPDLGTKNSSGGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 60

Query: 131 TVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLK 190
            VTKSLK+LQ KGL+K+VQHYQNKGKKFY+S DFEP+K LTGG+WYRDG LDK+YINTLK
Sbjct: 61  VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 120

Query: 191 GVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGE 250
            VCARMIQ+KK++TIEGIVESFK+SGA KVELS+EQI+EIVNALVLD++VMEVKS+G GE
Sbjct: 121 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELSREQIEEIVNALVLDNQVMEVKSSGFGE 180

Query: 251 FDFMPVGKICYKFCSK-GGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKW 306
           F+F+P+GKICYK+C++ GG+KGEPK GAMASIPC VCP+I+YCTPNGIISP TCVYYTKW
Sbjct: 181 FEFIPIGKICYKYCTQGGGLKGEPKIGAMASIPCGVCPRITYCTPNGIISPATCVYYTKW 240

[Close]

BLAST of Lag0020991 vs. ExPASy TrEMBL
Match: A0A5D3D7H7 (DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00330 PE=3 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 3.8e-107
Identity = 192/243 (79.01%), Postives = 221/243 (90.95%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE-------GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAES 130
           MS+SQGPSSLKRKRP+       GGALI  EK+LYDLICSKQSMG+CTFDMKRET FAES
Sbjct: 5   MSKSQGPSSLKRKRPDLGTKNSSGGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 64

Query: 131 TVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLK 190
            VTKSLK+LQ KGL+K+VQHYQNKGKKFY+S DFEP+K LTGG+WYRDG LDK+YINTLK
Sbjct: 65  VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 124

Query: 191 GVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGE 250
            VCARMIQ+KK++TIEGIVESFK+SGA KVELS+EQI+EIVNALVLD++VMEVKS+G GE
Sbjct: 125 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELSREQIEEIVNALVLDNQVMEVKSSGFGE 184

Query: 251 FDFMPVGKICYKFCSK-GGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKW 306
           F+F+P+GKICYK+C++ GG+KGEPK GAMASIPC VCP+I+YCTPNGIISP TCVYYTKW
Sbjct: 185 FEFIPIGKICYKYCTQGGGLKGEPKIGAMASIPCGVCPRITYCTPNGIISPATCVYYTKW 244

[Close]

BLAST of Lag0020991 vs. ExPASy TrEMBL
Match: A0A6J1D8D1 (DNA-directed RNA polymerase III subunit rpc6 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017972 PE=3 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 6.5e-107
Identity = 191/238 (80.25%), Postives = 219/238 (92.02%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE---GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAESTVTK 130
           MSRSQGPSSLKRKRP+   GGALIEHEK+LYDLICSKQSMG+   D+KRET +A+S VTK
Sbjct: 1   MSRSQGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTK 60

Query: 131 SLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLKGVCA 190
           SLKALQAKG++K+V+HYQNKGKKFY+SKDFEPSK LTGG+WY +G LD+EYINTLKG C 
Sbjct: 61  SLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCG 120

Query: 191 RMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGEFDFM 250
           RMIQK K+ TIEGIV+SFK+SGASKV+LS+EQIQEIVNALVLD+EVMEVKS G+GEFDF+
Sbjct: 121 RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFI 180

Query: 251 PVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWLDF 306
           P+GKICYK  +KGGVKGEPKTGAMASIPC VCP+IS+CTPNG+ISP+TCVYYTKWLDF
Sbjct: 181 PIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 238

[Close]

BLAST of Lag0020991 vs. ExPASy TrEMBL
Match: A0A5D3CS47 (DNA-directed RNA polymerase III subunit RPC6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006860 PE=3 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 6.5e-107
Identity = 190/242 (78.51%), Postives = 220/242 (90.91%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE-------GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAES 130
           MS+SQGPSSLKRKRP+        GALI  EK+LYDLICSKQSMG+CTFDMKRET FAES
Sbjct: 117 MSKSQGPSSLKRKRPDLSTKNSSVGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 176

Query: 131 TVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLK 190
            VTKSLK+LQ KGL+K+VQHYQNKGKKFY+S DFEP+K LTGG+WYRDG LDK+YINTLK
Sbjct: 177 VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 236

Query: 191 GVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGE 250
            VCARMIQ+KK++TIEGIVESFK+SGA KVEL++EQI+EIVNALVLD++V EVKS+G GE
Sbjct: 237 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELTREQIEEIVNALVLDNQVTEVKSSGFGE 296

Query: 251 FDFMPVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWL 306
           F+F+P+GKICYK+CS+GG+KGEPKTGAMASIPC VCP+I++CTPNGIISP TCVYYTKWL
Sbjct: 297 FEFIPIGKICYKYCSQGGLKGEPKTGAMASIPCGVCPRITHCTPNGIISPATCVYYTKWL 356

[Close]

BLAST of Lag0020991 vs. ExPASy TrEMBL
Match: A0A5A7UBI4 (DNA-directed RNA polymerase III subunit RPC6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003590 PE=3 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 6.5e-107
Identity = 190/242 (78.51%), Postives = 220/242 (90.91%), Query Frame = 0


Query: 71  MSRSQGPSSLKRKRPE-------GGALIEHEKMLYDLICSKQSMGMCTFDMKRETNFAES 130
           MS+SQGPSSLKRKRP+        GALI  EK+LYDLICSKQSMG+CTFDMKRET FAES
Sbjct: 117 MSKSQGPSSLKRKRPDLSTKNSSVGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 176

Query: 131 TVTKSLKALQAKGLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLK 190
            VTKSLK+LQ KGL+K+VQHYQNKGKKFY+S DFEP+K LTGG+WYRDG LDK+YINTLK
Sbjct: 177 VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 236

Query: 191 GVCARMIQKKKMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGE 250
            VCARMIQ+KK++TIEGIVESFK+SGA KVEL++EQI+EIVNALVLD++V EVKS+G GE
Sbjct: 237 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELTREQIEEIVNALVLDNQVTEVKSSGFGE 296

Query: 251 FDFMPVGKICYKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWL 306
           F+F+P+GKICYK+CS+GG+KGEPKTGAMASIPC VCP+I++CTPNGIISP TCVYYTKWL
Sbjct: 297 FEFIPIGKICYKYCSQGGLKGEPKTGAMASIPCGVCPRITHCTPNGIISPATCVYYTKWL 356

[Close]

BLAST of Lag0020991 vs. TAIR 10
Match: AT5G23710.1 (DNA binding;DNA-directed RNA polymerases )

HSP 1 Score: 201.1 bits (510), Expect = 1.3e-51
Identity = 104/232 (44.83%), Postives = 146/232 (62.93%), Query Frame = 0


Query: 81  KRKRPE----GGALIE-HEKMLYDLICSKQSMGMCTFDMKRETNFAESTVTKSLKALQAK 140
           KRKRP+    GG L E HEK L DLI   Q  G   F++KRE     + VT+ + +L+ K
Sbjct: 3   KRKRPDPKSSGGDLNEPHEKKLLDLIRINQGRGATMFELKREKTIPATIVTRLIASLRKK 62

Query: 141 GLVKVVQHYQNKGKKFYMSKDFEPSKALTGGDWYRDGILDKEYINTLKGVCARMIQ--KK 200
            L+K V +  NKG K Y++ +FEP   LTGG+WY DG LD   I  LK  C  +++  ++
Sbjct: 63  NLIKEVANMNNKGVKHYLAMEFEPCSELTGGEWYTDGALDLSKIEDLKAKCVMILERHRQ 122

Query: 201 KMITIEGIVESFKRSGASKVELSKEQIQEIVNALVLDHEVMEVKSNGTGEFDFMPVGKIC 260
           +++T+E +   F      + +LS +Q +EI+  L+LD+ +MEVKSNG  EF    +G++C
Sbjct: 123 RVVTLEVLCGYF----VKEEKLSVDQTKEILKNLILDNLIMEVKSNGMNEFASTRIGEVC 182

Query: 261 YKFCSKGGVKGEPKTGAMASIPCAVCPQISYCTPNGIISPKTCVYYTKWLDF 306
           Y+   K    GEP+ GA ASIPC VCP I+ C+P+G+ISP TCVY+ KWLDF
Sbjct: 183 YRLTGKKFGNGEPRAGAFASIPCGVCPHIAICSPDGVISPTTCVYFQKWLDF 230

The following BLAST results are available for this feature:

BLAST of Lag0020991 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK19400.1	7.9e-107	79.01	DNA-directed RNA polymerase III subunit rpc6-like [Cucumis melo var. makuwa]	[more]
XP_008448982.1	7.9e-107	79.01	PREDICTED: DNA-directed RNA polymerase III subunit rpc6-like [Cucumis melo] >XP_...	[more]
TYK13079.1	1.3e-106	78.51	DNA-directed RNA polymerase III subunit RPC6-like [Cucumis melo var. makuwa]	[more]
XP_008439941.1	1.3e-106	78.51	PREDICTED: DNA-directed RNA polymerase III subunit RPC6-like [Cucumis melo]	[more]
XP_022149571.1	1.3e-106	80.25	DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia] >X...	[more]

BLAST of Lag0020991 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q86JM3	9.6e-23	28.75	DNA-directed RNA polymerase III subunit rpc6 OS=Dictyostelium discoideum OX=4468...	[more]
Q921X6	3.6e-22	30.25	DNA-directed RNA polymerase III subunit RPC6 OS=Mus musculus OX=10090 GN=Polr3f ...	[more]
Q2T9S3	8.1e-22	30.25	DNA-directed RNA polymerase III subunit RPC6 OS=Bos taurus OX=9913 GN=POLR3F PE=...	[more]
Q9H1D9	8.1e-22	30.25	DNA-directed RNA polymerase III subunit RPC6 OS=Homo sapiens OX=9606 GN=POLR3F P...	[more]
P91529	1.3e-16	27.82	Probable DNA-directed RNA polymerase III subunit RPC6 OS=Caenorhabditis elegans ...	[more]

BLAST of Lag0020991 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BL06	3.8e-107	79.01	DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5D3D7H7	3.8e-107	79.01	DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo var. makuwa OX...	[more]
A0A6J1D8D1	6.5e-107	80.25	DNA-directed RNA polymerase III subunit rpc6 isoform X1 OS=Momordica charantia O...	[more]
A0A5D3CS47	6.5e-107	78.51	DNA-directed RNA polymerase III subunit RPC6-like OS=Cucumis melo var. makuwa OX...	[more]
A0A5A7UBI4	6.5e-107	78.51	DNA-directed RNA polymerase III subunit RPC6-like OS=Cucumis melo var. makuwa OX...	[more]

BLAST of Lag0020991 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23710.1	1.3e-51	44.83	DNA binding;DNA-directed RNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007832	RNA polymerase Rpc34	PFAM	PF05158	RNA_pol_Rpc34	coord: 91..198 e-value: 1.2E-24 score: 87.3
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 92..155 e-value: 7.0E-15 score: 56.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 43..86
IPR016049	RNA polymerase Rpc34-like	PANTHER	PTHR12780	RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED	coord: 88..305
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 92..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0020991.1	Lag0020991.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006383	transcription by RNA polymerase III
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0008168	methyltransferase activity