Lag0020158 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020158
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionYpt/Rab-GAP domain of gyp1p superfamily protein isoform 2
Locationchr5: 48487573 .. 48491106 (-)
RNA-Seq ExpressionLag0020158
SyntenyLag0020158
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGCTCTTAAACGAACTCCAACTCCGTCGTCTCCGGATCCGAATTCGAATTCTCCTCCTTCTTCATCCTCCTCGTCGTCAGCCTCGTCCTCAGCGTCATCGTCATGGGTTCATTTGCGTTCGGTTCTATTCGTTGTCACTTCCTCTTCACCGGCTTCTTCTTCCTCCTCTGATCGGTGAGTCTCTCTTTCCTTCCTCTTTTGCTTCAGATTTCTCTTATTCAGCTCAAAATTAAGCATAAGCCTAGTTTTCGACAGAGATCTTGGGCGCTTCGGGTTTTAGGCGTCTTTCTAGGGCTGATCTTTTGCTCAATCTAGCTGTTTAGGTCGTCTGAGTTCCATGTCTCCTGATTCTGGAGTATGTAGTTTCCTTTCTCACATTGTGGAGTTACTATCTAAATATCCTGTCTGCTTCGGTCTTTTAGAGTACTCGTTTTAGTCATCTTTTATTTTTCAACTATAACTGGTGAATTATTAGTGTCGTCATCCTTCTTCTTAGAAGGAGATACATAGATAGGTAATTGTGTATCATGCTGAGGGGACGCCATTTCATTGGATAAGATTAAAATGAACTGACTTCCATATATTAACCATGTCTTAGGGGACGGCTTAAATCACCATGGTCACGCAAGAAAAGAAAGCACGCACTTTCACCGCAACAATGGAGGAGTTTGTTTACGCCAGATGGGAAACTTAGAGATGGCGGCATTAAGTTTCTAAAAAAAGTTCGCAGTGGAGTGAGTAAAAATCACTTTGCCTCACGCATTTTCTTTCAATAGTTGGAAGTTTTTTTCTTAACTTCAAGATATTCACTGATTGGATTTTGATCTGATGATTTCTATTAGGGTGTAGATCCAAGTATTAGAGCAGAGGTCTGGCCGTTCCTTCTTGGAGTGTAAGTGAATCCCCACCCCCTTTCATTTTGTAATCTTGCAAATGCTTCTTCTCTTGTATTTTGTACAAAATATTTTAGGTCTCTAGTCCATGGGGGATCTGGTTAGAATGCTGGTTCTTCTGGCTCCTTACAAAGTGCATCATCTTAACACATATCTATTAGCTATTTTATTTCAGAACCTCAAATTCTGTGGCCATGTAACCTTAAGAAAATATCTAGCATGGTTTGTTGATCTTTTCCAAAACTTCCCTTCTCAAATGTTTTTTGAGTTGAAAACATAAATTCTTGACTCTTGGGTATTGGGTATTAATTTGTTTGGTCTTGTCTCCTTTGTTCTTTTATCCTTTTGACCAATGTGTGTGGGCAAGAAAGGAATGTTTGATGCAGGTTTCTTTCTATCTAGTTCATTATTATTGCTATTATATCATCATCCTTGTGACTTTTATAATCTCAGCCTCTTCTTATTGCATATCCGTGCCAGCTAGATGTCTCTTTGAGATCCACCCCTATTCAGCTTCCCCCTCTCTCACTAAAGTCTACACAAGCTGTATTATACGATAGCAAGGGTATATTTTTGCTCTAACCATGATAAATGTCTGAGTAATCAATATGTCCCAATGAGTTTCAAAGTATGTCCTAGTATGCCTGCAATTCTAAAATTAATAAAAGGTTTGAAGTTCTGCTTGTTGGTGACTTGATCACAGTGTTTTGGAATCAATGATCTGCCAAACTTGGAGAGACAGTGTTACATAGGTTTGTATCGTATAAACATAGAAGGGTATAAAGCTATTCCTGCAAAAAAAAAAAAAAAAAGGGATGCTGCATTCTTATGTGTCTTCCCAGCATATAACCTTTTCTCCTTTGTCTGTGCTCATTTTGTTAACTTTTTATTAACGATTGGAAGTCATTACTTTTATAGTCTTTTGGGGGCTGGGTTCTCTCTACACATGGCCCCTAGGTTGTTTTTTGTTTTGTCGTTGTACATTCACCATTTCTATAGGACTAGTCATAGCGAAACACCCTCATTGTAAAGATCCTAGTCATGCTATGTATACATGCTGTGCTACTATGCTATGTGTATGTTTATCATCATGTGGGGTGCAAAATGTGTATTGACATAGCTACTTGTGTTTTTCAACAGCTACGACTTGAGCAGTTCTGAAGAAGAAAGAGATGCTGTAAGAGTACAGAAGAGGTATATGTTCTCAAGTGCTTATGTTATTTCATTCTCTTTTTCTTAGATAAGTGTGTGGTAGTATTTTACCCACCTGTAGCTGTGTTCTGAGTTGGCATTTTAAACTTTAAGGTGATCAACATCTATAATGCAGGAAAGAATATGAAAAACTTCGAAAACAATGCCGATCTTTACTGAAGTTTGGGGATGAGAGTATAAAGTTGAATGATGATGAGATGAACTGCAACAAGGAGAGGGACAATCAGCATGTTGCTCATTGTGATGACTCTCCGATGAACTGCAACAAGGAGGGGAACAATCAGCATGTTGCTGATGGTGATGACTCTCCTAGTTTGGAAGATGTGGTTAGTGCCAGAGAATCTATTTCTAGTGATGAAAGGGGCCCAGGCTTTAGATACTTGGATGGAACCTCGGGGGTTTTGTTGGAAGAGGATGACAGTTCTAGACGGATGACAAACGCTGATGCTTCTCTTCTTAATACTGAATCATCTGACTCAGATTCATCTGAGGATCCTGAAGTCAGTCAAACCTTCCCTTCCTCTGATGGTAGAGAAGATAATGATCCTGATTTCACTTCCAAAAATTCGTCTCCCTTGGTAACAGAGGTTACATCCAAATTTCGCAGTAATGAAGACTTTACAACATGGCAGCGGATAATCCGTCTTGATGCAGTACGTGCAAATGCAGAATGGATAGCATATGCACCATCCCAAGCAGCGGTATCAGATGATAAGGCTAGACATTCTGCTGAGGTTGTTGGTTTGAAAGATTACGATCACCTAGATTCCTGCAGGATCTTTCATGCTGCTAGGCTGGTCACCATTCTTGAAGCATATGCTCTTTATGATCCTGAAATTGGGTATTGCCAAGGAATGAGTGATCTGCTTTCTCCTATAATTACTGTGATAACTGAAGATCATGAGGCATTCTGGTGCTTTGTGGGCTTCATGCGGAAAGCTCGGCATAACTTTAGGCTTGATGAGGTTGGGATTCGAAAGCAACTGAACATTGTATCTAGAATCATCAAATTCAAGGACTCCCACCTTTACAGACACCTGCAAGACCTCCAAGCAGAGGATTGCTTTTTCGTTTATAGGATGGTCGTGGTACTCTTTAGAAGGGAATTAACATTCGAACAGACCCTGTGCCTTTGGGAGGTCATGTGGGCTGATCAGGCAGCTATTAGAGCTGGTGTAGGTAAATCAGCTTGGAGCAGGATTAGGCAACGAGCCCCACCCACGGAGGATCTGCTGCTCTATGCAATTGCTGCCTCAGTATTGCAGAAGAGGAAGCTGATTATAGAGAGATACAACAGCATGGACGAAATAATAAGGGAGTGTAACAGCATGGCCGGGCAACTTGATGTATGGAAACTATTGGACGATGCTCATGATTTGGTGGTGACCCTCCATGAGAAGATAGAAACCTCGTTTAACGAGTAA

mRNA sequence

ATGAGAGCTCTTAAACGAACTCCAACTCCGTCGTCTCCGGATCCGAATTCGAATTCTCCTCCTTCTTCATCCTCCTCGTCGTCAGCCTCGTCCTCAGCGTCATCGTCATGGGTTCATTTGCGTTCGGTTCTATTCGTTGTCACTTCCTCTTCACCGGCTTCTTCTTCCTCCTCTGATCGGGGACGGCTTAAATCACCATGGTCACGCAAGAAAAGAAAGCACGCACTTTCACCGCAACAATGGAGGAGTTTGTTTACGCCAGATGGGAAACTTAGAGATGGCGGCATTAAGTTTCTAAAAAAAGTTCGCAGTGGAGGTGTAGATCCAAGTATTAGAGCAGAGGTCTGGCCGTTCCTTCTTGGAGTCTACGACTTGAGCAGTTCTGAAGAAGAAAGAGATGCTGTAAGAGTACAGAAGAGGAAAGAATATGAAAAACTTCGAAAACAATGCCGATCTTTACTGAAGTTTGGGGATGAGAGTATAAAGTTGAATGATGATGAGATGAACTGCAACAAGGAGAGGGACAATCAGCATGTTGCTCATTGTGATGACTCTCCGATGAACTGCAACAAGGAGGGGAACAATCAGCATGTTGCTGATGGTGATGACTCTCCTAGTTTGGAAGATGTGGTTAGTGCCAGAGAATCTATTTCTAGTGATGAAAGGGGCCCAGGCTTTAGATACTTGGATGGAACCTCGGGGGTTTTGTTGGAAGAGGATGACAGTTCTAGACGGATGACAAACGCTGATGCTTCTCTTCTTAATACTGAATCATCTGACTCAGATTCATCTGAGGATCCTGAAGTCAGTCAAACCTTCCCTTCCTCTGATGGTAGAGAAGATAATGATCCTGATTTCACTTCCAAAAATTCGTCTCCCTTGGTAACAGAGGTTACATCCAAATTTCGCAGTAATGAAGACTTTACAACATGGCAGCGGATAATCCGTCTTGATGCAGTACGTGCAAATGCAGAATGGATAGCATATGCACCATCCCAAGCAGCGGTATCAGATGATAAGGCTAGACATTCTGCTGAGGTTGTTGGTTTGAAAGATTACGATCACCTAGATTCCTGCAGGATCTTTCATGCTGCTAGGCTGGTCACCATTCTTGAAGCATATGCTCTTTATGATCCTGAAATTGGGTATTGCCAAGGAATGAGTGATCTGCTTTCTCCTATAATTACTGTGATAACTGAAGATCATGAGGCATTCTGGTGCTTTGTGGGCTTCATGCGGAAAGCTCGGCATAACTTTAGGCTTGATGAGGTTGGGATTCGAAAGCAACTGAACATTGTATCTAGAATCATCAAATTCAAGGACTCCCACCTTTACAGACACCTGCAAGACCTCCAAGCAGAGGATTGCTTTTTCGTTTATAGGATGGTCGTGGTACTCTTTAGAAGGGAATTAACATTCGAACAGACCCTGTGCCTTTGGGAGGTCATGTGGGCTGATCAGGCAGCTATTAGAGCTGGTGTAGGTAAATCAGCTTGGAGCAGGATTAGGCAACGAGCCCCACCCACGGAGGATCTGCTGCTCTATGCAATTGCTGCCTCAGTATTGCAGAAGAGGAAGCTGATTATAGAGAGATACAACAGCATGGACGAAATAATAAGGGAGTGTAACAGCATGGCCGGGCAACTTGATGTATGGAAACTATTGGACGATGCTCATGATTTGGTGGTGACCCTCCATGAGAAGATAGAAACCTCGTTTAACGAGTAA

Coding sequence (CDS)

ATGAGAGCTCTTAAACGAACTCCAACTCCGTCGTCTCCGGATCCGAATTCGAATTCTCCTCCTTCTTCATCCTCCTCGTCGTCAGCCTCGTCCTCAGCGTCATCGTCATGGGTTCATTTGCGTTCGGTTCTATTCGTTGTCACTTCCTCTTCACCGGCTTCTTCTTCCTCCTCTGATCGGGGACGGCTTAAATCACCATGGTCACGCAAGAAAAGAAAGCACGCACTTTCACCGCAACAATGGAGGAGTTTGTTTACGCCAGATGGGAAACTTAGAGATGGCGGCATTAAGTTTCTAAAAAAAGTTCGCAGTGGAGGTGTAGATCCAAGTATTAGAGCAGAGGTCTGGCCGTTCCTTCTTGGAGTCTACGACTTGAGCAGTTCTGAAGAAGAAAGAGATGCTGTAAGAGTACAGAAGAGGAAAGAATATGAAAAACTTCGAAAACAATGCCGATCTTTACTGAAGTTTGGGGATGAGAGTATAAAGTTGAATGATGATGAGATGAACTGCAACAAGGAGAGGGACAATCAGCATGTTGCTCATTGTGATGACTCTCCGATGAACTGCAACAAGGAGGGGAACAATCAGCATGTTGCTGATGGTGATGACTCTCCTAGTTTGGAAGATGTGGTTAGTGCCAGAGAATCTATTTCTAGTGATGAAAGGGGCCCAGGCTTTAGATACTTGGATGGAACCTCGGGGGTTTTGTTGGAAGAGGATGACAGTTCTAGACGGATGACAAACGCTGATGCTTCTCTTCTTAATACTGAATCATCTGACTCAGATTCATCTGAGGATCCTGAAGTCAGTCAAACCTTCCCTTCCTCTGATGGTAGAGAAGATAATGATCCTGATTTCACTTCCAAAAATTCGTCTCCCTTGGTAACAGAGGTTACATCCAAATTTCGCAGTAATGAAGACTTTACAACATGGCAGCGGATAATCCGTCTTGATGCAGTACGTGCAAATGCAGAATGGATAGCATATGCACCATCCCAAGCAGCGGTATCAGATGATAAGGCTAGACATTCTGCTGAGGTTGTTGGTTTGAAAGATTACGATCACCTAGATTCCTGCAGGATCTTTCATGCTGCTAGGCTGGTCACCATTCTTGAAGCATATGCTCTTTATGATCCTGAAATTGGGTATTGCCAAGGAATGAGTGATCTGCTTTCTCCTATAATTACTGTGATAACTGAAGATCATGAGGCATTCTGGTGCTTTGTGGGCTTCATGCGGAAAGCTCGGCATAACTTTAGGCTTGATGAGGTTGGGATTCGAAAGCAACTGAACATTGTATCTAGAATCATCAAATTCAAGGACTCCCACCTTTACAGACACCTGCAAGACCTCCAAGCAGAGGATTGCTTTTTCGTTTATAGGATGGTCGTGGTACTCTTTAGAAGGGAATTAACATTCGAACAGACCCTGTGCCTTTGGGAGGTCATGTGGGCTGATCAGGCAGCTATTAGAGCTGGTGTAGGTAAATCAGCTTGGAGCAGGATTAGGCAACGAGCCCCACCCACGGAGGATCTGCTGCTCTATGCAATTGCTGCCTCAGTATTGCAGAAGAGGAAGCTGATTATAGAGAGATACAACAGCATGGACGAAATAATAAGGGAGTGTAACAGCATGGCCGGGCAACTTGATGTATGGAAACTATTGGACGATGCTCATGATTTGGTGGTGACCCTCCATGAGAAGATAGAAACCTCGTTTAACGAGTAA

Protein sequence

MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHVAHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLEEDDSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE
Homology
BLAST of Lag0020158 vs. NCBI nr
Match: XP_022141059.1 (GTPase-activating protein GYP7-like isoform X1 [Momordica charantia])

HSP 1 Score: 1002.7 bits (2591), Expect = 1.3e-288
Identity = 530/583 (90.91%), Postives = 545/583 (93.48%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSA--------SSSWVHLRSVLFVVTSSSP 60
           MRALKRT TPSSPDPNSNSPPSSSSSSSASSSA        SSSWVHLRSVLFVVTSSSP
Sbjct: 1   MRALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSP 60

Query: 61  ASSSSSDRGRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIR 120
           ASSSSSDRGRLKSPWSRKKRK+AL+PQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIR
Sbjct: 61  ASSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIR 120

Query: 121 AEVWPFLLGVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNK 180
           AEVWPFLLGVYDLSSSEEERDAVR+QKRKEYE+LRKQCR LLK GDES KLNDD+MN NK
Sbjct: 121 AEVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNK 180

Query: 181 ERDNQHVAHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGT 240
           ERD       +D   N NKEG+NQ+VA GDD PSLE+VVSARESISSDERG  FR LDGT
Sbjct: 181 ERDEN--IKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGT 240

Query: 241 SGVLLEEDDSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSS 300
           SGV LEEDDSSRRMT+ADAS  NTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSS
Sbjct: 241 SGVFLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSS 300

Query: 301 PLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKD 360
           P V EVTSKFRSNEDFTTWQRIIRLDAVRAN+EWIAYAPSQAAVSDDKARHSAEVVGLKD
Sbjct: 301 PSVAEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKD 360

Query: 361 YDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFM 420
           YDHL+  RIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED EAFWCFVGFM
Sbjct: 361 YDHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFM 420

Query: 421 RKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELT 480
           RKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHL+ LQAEDCFFVYRMVVVLFRRELT
Sbjct: 421 RKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELT 480

Query: 481 FEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERY 540
           FEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE+Y
Sbjct: 481 FEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKY 540

Query: 541 NSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 576
           NSMDEIIRECNSMAGQLD+WKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 541 NSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 581

BLAST of Lag0020158 vs. NCBI nr
Match: XP_022968472.1 (small G protein signaling modulator 2-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 998.0 bits (2579), Expect = 3.3e-287
Identity = 530/591 (89.68%), Postives = 545/591 (92.22%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60
           MRALK+TPTPSSPDPNSNSPPSSSS     SSASSSWVHLRSVLFVVTSSSPASSSSSDR
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSS----LSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60

Query: 61  GRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 120
           GRLKSPWSRKKRKHALSPQQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLL
Sbjct: 61  GRLKSPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLL 120

Query: 121 GVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDE------------- 180
           GVYDL+SSEEERDAVRVQKRKEYE+LRKQCRSLLKFGDES+KLNDD+             
Sbjct: 121 GVYDLTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESMKLNDDDMNCHKEGGIQHAA 180

Query: 181 ------MNCNKERDNQHVAHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDE 240
                 MNCNKE DNQHVAH DDS MNCNKEG+NQHVA GDDSPSL DV+SARESISSDE
Sbjct: 181 HGDGSSMNCNKEGDNQHVAHGDDSSMNCNKEGDNQHVAHGDDSPSLGDVISARESISSDE 240

Query: 241 RGPGFRYLDGTSGVLLEEDDSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGRED 300
           RG         SGVLLEEDDSSR MTNAD+S+LNTESSDSDSS DPEVS TFPSSDGRED
Sbjct: 241 RGL-------NSGVLLEEDDSSRGMTNADSSVLNTESSDSDSSGDPEVSHTFPSSDGRED 300

Query: 301 NDPDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKA 360
           NDPDFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKA
Sbjct: 301 NDPDFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKA 360

Query: 361 RHSAEVVGLKDYDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED 420
           RH AEVVGLKDYDHL+S RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED
Sbjct: 361 RHLAEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED 420

Query: 421 HEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYR 480
           HEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQ+L+AEDCFFVYR
Sbjct: 421 HEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQNLEAEDCFFVYR 480

Query: 481 MVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASV 540
           MVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASV
Sbjct: 481 MVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASV 540

Query: 541 LQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 573
           LQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 541 LQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 579

BLAST of Lag0020158 vs. NCBI nr
Match: XP_022923060.1 (GTPase-activating protein GYP7 isoform X1 [Cucurbita moschata])

HSP 1 Score: 997.3 bits (2577), Expect = 5.5e-287
Identity = 525/575 (91.30%), Postives = 536/575 (93.22%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60
           MRALKRTPT SSPDPNSNSPPSSSS SSA SSASSSWVHLRSVLFVVTSSSPASSSSSDR
Sbjct: 1   MRALKRTPTSSSPDPNSNSPPSSSSPSSALSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60

Query: 61  GRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 120
           GRLKSPWSRKKRKHALSPQQWRSLFTPDG+LRDGGIKFLKKVRSGGVDPSIRAEVWPFLL
Sbjct: 61  GRLKSPWSRKKRKHALSPQQWRSLFTPDGRLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 120

Query: 121 GVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHVA 180
           G YDLSSSEEERDAVRVQKRKEYE+LRKQCRSLLKFGD  IKLNDDEMN NK  D Q V+
Sbjct: 121 GFYDLSSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDGCIKLNDDEMNFNKVGDAQLVS 180

Query: 181 HCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLEED 240
           H                   GDDSPSLEDV SARESISSDERG  FRYLDGTS VLLEED
Sbjct: 181 H-------------------GDDSPSLEDVGSARESISSDERGTNFRYLDGTSEVLLEED 240

Query: 241 DSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPLVTEVTS 300
           DSSR+MTNAD S+LNTESSDSDSSEDPEVSQTFPSSDGREDNDP FTSKNSSPLVTEVTS
Sbjct: 241 DSSRQMTNADVSILNTESSDSDSSEDPEVSQTFPSSDGREDNDPVFTSKNSSPLVTEVTS 300

Query: 301 KFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLDSCR 360
           KFR NEDFTTWQRIIRLDAVRANAEWIAYAPS AAVSDDKARHSAEVVGLKDYDHL+SCR
Sbjct: 301 KFRGNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDKARHSAEVVGLKDYDHLESCR 360

Query: 361 IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR 420
           IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR
Sbjct: 361 IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR 420

Query: 421 LDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLW 480
           LDEVGIRKQLN+VS+II+FKDSHLYRHLQDL+AEDCFFVYRMVVVLFRRELTFEQTLCLW
Sbjct: 421 LDEVGIRKQLNVVSKIIRFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQTLCLW 480

Query: 481 EVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERYNSMDEIIR 540
           EVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE+Y SMDEIIR
Sbjct: 481 EVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYYSMDEIIR 540

Query: 541 ECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 576
           ECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 541 ECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 556

BLAST of Lag0020158 vs. NCBI nr
Match: XP_038896358.1 (GTPase-activating protein GYP7-like isoform X1 [Benincasa hispida])

HSP 1 Score: 994.6 bits (2570), Expect = 3.6e-286
Identity = 527/579 (91.02%), Postives = 539/579 (93.09%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPA--SSSSS 60
           MRALKRTPT SSPDPNSNSPPSSSSS   SSSASSSWVHLRSVLFVVTSSSPA  SSSSS
Sbjct: 1   MRALKRTPTSSSPDPNSNSPPSSSSS---SSSASSSWVHLRSVLFVVTSSSPASSSSSSS 60

Query: 61  DRGRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPF 120
           DRGRLKSPWSRKKRKHALSPQQWR LFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPF
Sbjct: 61  DRGRLKSPWSRKKRKHALSPQQWRGLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPF 120

Query: 121 LLGVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQH 180
           LLG YDLSSSEEERDAVRVQKRKEYE+LRKQCRSLLKFG ESIKL+DDE           
Sbjct: 121 LLGFYDLSSSEEERDAVRVQKRKEYERLRKQCRSLLKFGAESIKLDDDE----------- 180

Query: 181 VAHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLE 240
                   MNCNKEG NQHVA GDDSP+LEDVVSARESI SDE+   FRYLDGTSGVLLE
Sbjct: 181 --------MNCNKEGGNQHVAHGDDSPTLEDVVSARESIYSDEKASNFRYLDGTSGVLLE 240

Query: 241 EDDSSRRMT--NADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPLVT 300
           EDDSSRRMT  +ADAS+LNTESSDSDSSEDPEVSQTFPSSDGREDNDP+FTS NSSPLVT
Sbjct: 241 EDDSSRRMTSVDADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNDPEFTSNNSSPLVT 300

Query: 301 EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHL 360
           EVTSKFR NEDFTTWQRIIRLDAVRANAEWIAYAPS AAVSDD+ARH AEVVGLKDYDHL
Sbjct: 301 EVTSKFRCNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDEARHCAEVVGLKDYDHL 360

Query: 361 DSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKAR 420
           +SCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKAR
Sbjct: 361 ESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKAR 420

Query: 421 HNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQT 480
           HNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDL+AEDCFFVYRMVVVLFRRELTFEQT
Sbjct: 421 HNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQT 480

Query: 481 LCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERYNSMD 540
           LCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE+YNSMD
Sbjct: 481 LCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMD 540

Query: 541 EIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 576
           EIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSF+E
Sbjct: 541 EIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFDE 557

BLAST of Lag0020158 vs. NCBI nr
Match: KAG7013330.1 (GYP7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 994.6 bits (2570), Expect = 3.6e-286
Identity = 528/591 (89.34%), Postives = 545/591 (92.22%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60
           MRALK+TPTPSSPDPNSNSPPSSSS     SSASSSWVHLRSVLFVVTSSSPASSSSSDR
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSS----LSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60

Query: 61  GRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 120
           GRLKSPWSRKKRKHALSPQQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLL
Sbjct: 61  GRLKSPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLL 120

Query: 121 GVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDE------------- 180
           GVYDL+SSEEERDAVRVQKRKEYE+LRKQCRSLLKFGDES+KLNDD+             
Sbjct: 121 GVYDLTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESMKLNDDDMNCHKEGGIQHAA 180

Query: 181 ------MNCNKERDNQHVAHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDE 240
                 MNCNKE DNQHVAH DDS MNCNKEG+NQHVA GDDSPSL DV+SARESISSDE
Sbjct: 181 HGDDSSMNCNKEGDNQHVAHGDDSLMNCNKEGDNQHVAHGDDSPSLGDVISARESISSDE 240

Query: 241 RGPGFRYLDGTSGVLLEEDDSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGRED 300
           RG         SGVLLEEDDSSR MTNADAS+LNTESSDSDSS DPEVS TFPSSDGRE+
Sbjct: 241 RGL-------NSGVLLEEDDSSRGMTNADASVLNTESSDSDSSGDPEVSHTFPSSDGREE 300

Query: 301 NDPDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKA 360
           NDPDFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKA
Sbjct: 301 NDPDFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKA 360

Query: 361 RHSAEVVGLKDYDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED 420
           RH AEVVGLKDYDHL+S RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED
Sbjct: 361 RHLAEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED 420

Query: 421 HEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYR 480
           HEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRII+FKDSHLYRHLQ+L+AEDCFFVYR
Sbjct: 421 HEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIRFKDSHLYRHLQNLEAEDCFFVYR 480

Query: 481 MVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASV 540
           MVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASV
Sbjct: 481 MVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASV 540

Query: 541 LQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 573
           LQKRKLIIE+YNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 541 LQKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 579

BLAST of Lag0020158 vs. ExPASy Swiss-Prot
Match: Q94BY9 (Rab GTPase-activating protein 22 OS=Arabidopsis thaliana OX=3702 GN=RABGAP22 PE=1 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 7.5e-178
Identity = 356/581 (61.27%), Postives = 431/581 (74.18%), Query Frame = 0

Query: 3   ALKRTPTPSSPDPNSNSPPSSSS---SSSASSSASSSWVHLRSVLFVVTSSSPASSSSSD 62
           +L  + + S P  +S+SPPSS+S   S+S SSS+SSSW+HLRSVLFV   SSP+S +SSD
Sbjct: 21  SLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFVANLSSPSSVTSSD 80

Query: 63  RGRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 122
           R R KSPWSR+KRK AL+P QWRSLFTP+GKLRDGG+ FLKKVRS GVDPSIRAEVW FL
Sbjct: 81  R-RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFL 140

Query: 123 LGVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHV 182
           LGVYDL+S+ EER+AV+ QKRKEYEKL+++C+ LLK G             N   DN   
Sbjct: 141 LGVYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCG-------------NGSTDNLEE 200

Query: 183 AHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLEE 242
              D++   C +  ++  +     S   +DVVSA                          
Sbjct: 201 LPSDEANSQCVRFVDDYKITGPMTS---QDVVSA-------------------------- 260

Query: 243 DDSSRRMTNADASLLNTESSDSDSSEDPE---VSQTFPSSDGR---EDNDPDFTSKNSSP 302
                         LNT+SSD+DS ED E   +  +F  SD +   EDN  + + +NSS 
Sbjct: 261 --------------LNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSSL 320

Query: 303 LV---TEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGL 362
           LV   +EV  +   +EDF+TWQRIIRLDA+RA++EW  Y+P   A+++ KAR  AE VGL
Sbjct: 321 LVAAASEVQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITESKARRLAESVGL 380

Query: 363 KDYDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVG 422
           KDYDHL+SCR++HAARLV ILEAYA+YDPEIGYCQGMSDLLSPI+ VI+EDHEAFWCFVG
Sbjct: 381 KDYDHLESCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFVG 440

Query: 423 FMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRE 482
           FM+KARHNFRLDE GI++QL+IVS+IIK KDS LY+HL++LQAEDC FVYRMV+V+FRRE
Sbjct: 441 FMKKARHNFRLDEAGIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRRE 500

Query: 483 LTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE 542
           L+FEQTLCLWEVMWADQAAIRAGVGKS WSRIRQ+APPT+DLLLYAIAA VL +RKLII+
Sbjct: 501 LSFEQTLCLWEVMWADQAAIRAGVGKSPWSRIRQQAPPTDDLLLYAIAALVL-RRKLIIQ 543

Query: 543 RYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIET 572
           +Y+SMDEI+ ECNSMAGQL+VWKLLDDAH LVVTLH+KIET
Sbjct: 561 KYSSMDEIVEECNSMAGQLNVWKLLDDAHHLVVTLHDKIET 543

BLAST of Lag0020158 vs. ExPASy Swiss-Prot
Match: P09379 (GTPase-activating protein GYP7 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=GYP7 PE=3 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 1.3e-25
Identity = 63/208 (30.29%), Postives = 108/208 (51.92%), Query Frame = 0

Query: 363 HAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLD 422
           H  +L  +L  Y  ++  +GY QGMSDLLSP+  V+ +D  AFW F  FM +   N+  D
Sbjct: 495 HLIQLRDMLITYNQHNKNLGYVQGMSDLLSPLYVVLQDDTLAFWAFSAFMERMERNYLRD 554

Query: 423 EVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEV 482
           + G+R QL  +  +++F    LY+HL+  ++ + FF +RM++V F+REL ++  L LWEV
Sbjct: 555 QSGMRNQLLCLDHLVQFMLPSLYKHLEKTESTNLFFFFRMLLVWFKRELLWDDVLRLWEV 614

Query: 483 MWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERYNSMDEIIREC 542
           +W D                      +   +L+   A + + + ++I+     DEI++  
Sbjct: 615 LWTDYL--------------------SSQFVLFVCLAILDKHKDVMIDHLAGFDEILKYM 674

Query: 543 NSMAGQLDVWKLLDDAHDLVVTLHEKIE 571
           N ++  +D+ +LL  A  L       +E
Sbjct: 675 NELSMTIDLDELLVRAELLFYRFRRTVE 682


HSP 2 Score: 48.5 bits (114), Expect = 2.9e-04
Identity = 29/93 (31.18%), Postives = 52/93 (55.91%), Query Frame = 0

Query: 58  SDRG-RLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVW 117
           SD G  L    +  +R++ +S  +W + F  +G+L     +  +++  GG+ P++R E W
Sbjct: 339 SDEGFELIDAENNPQRRNEVSLAEWNAFFDYNGRLIVTVNEVKERIFHGGLAPAVRPEGW 398

Query: 118 PFLLGVYDLSSSEEERDAVRVQKRKEYEKLRKQ 150
            FLLGVY   S+  ER  +  + R +Y +L+K+
Sbjct: 399 LFLLGVYPWDSTAAERKELVSKLRVDYNRLKKE 431

BLAST of Lag0020158 vs. ExPASy Swiss-Prot
Match: Q6FWI1 (GTPase-activating protein GYP7 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=GYP7 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 6.2e-23
Identity = 114/499 (22.85%), Postives = 186/499 (37.27%), Query Frame = 0

Query: 71  KRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGV-DPSIRAEVWPFLLGVYDLSSSE 130
           +R H ++ Q+W SLF  +G+L     +    +  GG+ D + R EVWPFLLGVY   SSE
Sbjct: 355 ERNHPMTKQKWNSLFDSEGRLTVTVNEVKDYIFHGGLADDATRKEVWPFLLGVYPWDSSE 414

Query: 131 EERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHVAHCDDSPMNC 190
           +ER  +R     EY +L++      K+ D  + L++DE    K++               
Sbjct: 415 DERKQLRKALHDEYMELKQ------KWVDREVNLDNDEEEYWKDQ--------------- 474

Query: 191 NKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLEEDDSSRRMTNA 250
                                                        +   E D  R   N 
Sbjct: 475 ---------------------------------------------LFRIEKDVKRNDRNI 534

Query: 251 DASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPLVTEVTSKFRSNEDFT 310
           D    NT    SD+   PE   T P++D                                
Sbjct: 535 DIYKYNT----SDNLPFPE--DTAPTTD-------------------------------- 594

Query: 311 TWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLDSCRIFHAARLVT 370
                                       DD                 DS +  +  +L  
Sbjct: 595 ----------------------------DD-----------------DSIKNPNLKKLAD 654

Query: 371 ILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQ 430
           IL  Y +++P +GY QGM+DLLSP+  +I ++   FWCF  FM +   NF  D+ GIR Q
Sbjct: 655 ILTTYNIFNPNLGYVQGMTDLLSPLYYIIRDEETTFWCFTNFMERMERNFLRDQSGIRDQ 683

Query: 431 LNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAA 490
           +  ++ + +     L  HLQ   + D FF +RM++V F+RE  ++    +WEV + D  +
Sbjct: 715 MLALTDLCQLMLPRLSAHLQKCDSSDLFFCFRMLLVWFKREFNYDDIFNIWEVFFTDFYS 683

Query: 491 IRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKL-IIERYNSMDEIIRECNSMAGQ 550
                              ++  L + +A  +LQK    I+    + D++I+  N +  +
Sbjct: 775 -------------------SQYQLFFMLA--ILQKNSSPIVNNLQTFDQVIKYFNDLNSK 683

Query: 551 LDVWKLLDDAHDLVVTLHE 568
           ++   L+  +  L +  H+
Sbjct: 835 MNWRDLMVRSELLFIQFHK 683

BLAST of Lag0020158 vs. ExPASy Swiss-Prot
Match: P48365 (GTPase-activating protein GYP7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GYP7 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.1e-22
Identity = 67/239 (28.03%), Postives = 110/239 (46.03%), Query Frame = 0

Query: 331 PSQAAVSDDKARHSAEVVGLKDYDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDL 390
           P Q   +++    S E    +  D  D  R  H   L  IL  Y +Y+  +GY QGM+DL
Sbjct: 477 PPQQLPANENNSTSPESANDESDDADDGVRNPHLIHLQNILITYNVYNTNLGYVQGMTDL 536

Query: 391 LSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQD 450
           LSPI  ++ E+ + FWCF  FM     NF  D+ GI +Q+  +  +++     L  HL  
Sbjct: 537 LSPIYVIMKEEWKTFWCFTHFMDIMERNFLRDQSGIHEQMLTLVELVQLMLPELSEHLNK 596

Query: 451 LQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTE 510
             + + FF +RM++V F+RE   E  + +WE  W                        + 
Sbjct: 597 CDSGNLFFCFRMLLVWFKREFEMEDIMHIWENFWTFYY--------------------SS 656

Query: 511 DLLLYAIAASVLQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKI 570
              L+ + A + +  + I++  N  D+I++  N + G+LD W  L    +L+    EK+
Sbjct: 657 QFQLFFMLAILQKNSQAILQHLNQFDQILKFFNELNGKLD-WNDLMVRAELLFKKFEKM 694

BLAST of Lag0020158 vs. ExPASy Swiss-Prot
Match: Q8TC07 (TBC1 domain family member 15 OS=Homo sapiens OX=9606 GN=TBC1D15 PE=1 SV=2)

HSP 1 Score: 109.4 bits (272), Expect = 1.4e-22
Identity = 61/212 (28.77%), Postives = 107/212 (50.47%), Query Frame = 0

Query: 351 KDYDHLDSCRIFHAAR-------LVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHE 410
           KD +  D    F+  +       L  IL  Y +YD ++GY QGMSDLLSP++ V+  + +
Sbjct: 413 KDVNRTDRTNKFYEGQDNPGLILLHDILMTYCMYDFDLGYVQGMSDLLSPLLYVMENEVD 472

Query: 411 AFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMV 470
           AFWCF  +M +   NF     G++ QL  +S +++  DS    +L+   +   +F +R +
Sbjct: 473 AFWCFASYMDQMHQNFEEQMQGMKTQLIQLSTLLRLLDSGFCSYLESQDSGYLYFCFRWL 532

Query: 471 VVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQ 530
           ++ F+RE +F   L LWEVMW +                     P  +  L    A +  
Sbjct: 533 LIRFKREFSFLDILRLWEVMWTEL--------------------PCTNFHLLLCCAILES 592

Query: 531 KRKLIIERYNSMDEIIRECNSMAGQLDVWKLL 556
           +++ I+E++   +EI++  N ++ ++DV  +L
Sbjct: 593 EKQQIMEKHYGFNEILKHINELSMKIDVEDIL 604

BLAST of Lag0020158 vs. ExPASy TrEMBL
Match: A0A6J1CHH5 (GTPase-activating protein GYP7-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011552 PE=4 SV=1)

HSP 1 Score: 1002.7 bits (2591), Expect = 6.4e-289
Identity = 530/583 (90.91%), Postives = 545/583 (93.48%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSA--------SSSWVHLRSVLFVVTSSSP 60
           MRALKRT TPSSPDPNSNSPPSSSSSSSASSSA        SSSWVHLRSVLFVVTSSSP
Sbjct: 1   MRALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSP 60

Query: 61  ASSSSSDRGRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIR 120
           ASSSSSDRGRLKSPWSRKKRK+AL+PQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIR
Sbjct: 61  ASSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIR 120

Query: 121 AEVWPFLLGVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNK 180
           AEVWPFLLGVYDLSSSEEERDAVR+QKRKEYE+LRKQCR LLK GDES KLNDD+MN NK
Sbjct: 121 AEVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNK 180

Query: 181 ERDNQHVAHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGT 240
           ERD       +D   N NKEG+NQ+VA GDD PSLE+VVSARESISSDERG  FR LDGT
Sbjct: 181 ERDEN--IKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGT 240

Query: 241 SGVLLEEDDSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSS 300
           SGV LEEDDSSRRMT+ADAS  NTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSS
Sbjct: 241 SGVFLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSS 300

Query: 301 PLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKD 360
           P V EVTSKFRSNEDFTTWQRIIRLDAVRAN+EWIAYAPSQAAVSDDKARHSAEVVGLKD
Sbjct: 301 PSVAEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKD 360

Query: 361 YDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFM 420
           YDHL+  RIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED EAFWCFVGFM
Sbjct: 361 YDHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFM 420

Query: 421 RKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELT 480
           RKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHL+ LQAEDCFFVYRMVVVLFRRELT
Sbjct: 421 RKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELT 480

Query: 481 FEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERY 540
           FEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE+Y
Sbjct: 481 FEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKY 540

Query: 541 NSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 576
           NSMDEIIRECNSMAGQLD+WKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 541 NSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 581

BLAST of Lag0020158 vs. ExPASy TrEMBL
Match: A0A6J1HTL1 (small G protein signaling modulator 2-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111467703 PE=4 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.6e-287
Identity = 530/591 (89.68%), Postives = 545/591 (92.22%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60
           MRALK+TPTPSSPDPNSNSPPSSSS     SSASSSWVHLRSVLFVVTSSSPASSSSSDR
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSS----LSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60

Query: 61  GRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 120
           GRLKSPWSRKKRKHALSPQQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLL
Sbjct: 61  GRLKSPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLL 120

Query: 121 GVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDE------------- 180
           GVYDL+SSEEERDAVRVQKRKEYE+LRKQCRSLLKFGDES+KLNDD+             
Sbjct: 121 GVYDLTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESMKLNDDDMNCHKEGGIQHAA 180

Query: 181 ------MNCNKERDNQHVAHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDE 240
                 MNCNKE DNQHVAH DDS MNCNKEG+NQHVA GDDSPSL DV+SARESISSDE
Sbjct: 181 HGDGSSMNCNKEGDNQHVAHGDDSSMNCNKEGDNQHVAHGDDSPSLGDVISARESISSDE 240

Query: 241 RGPGFRYLDGTSGVLLEEDDSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGRED 300
           RG         SGVLLEEDDSSR MTNAD+S+LNTESSDSDSS DPEVS TFPSSDGRED
Sbjct: 241 RGL-------NSGVLLEEDDSSRGMTNADSSVLNTESSDSDSSGDPEVSHTFPSSDGRED 300

Query: 301 NDPDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKA 360
           NDPDFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKA
Sbjct: 301 NDPDFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKA 360

Query: 361 RHSAEVVGLKDYDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED 420
           RH AEVVGLKDYDHL+S RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED
Sbjct: 361 RHLAEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED 420

Query: 421 HEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYR 480
           HEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQ+L+AEDCFFVYR
Sbjct: 421 HEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQNLEAEDCFFVYR 480

Query: 481 MVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASV 540
           MVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASV
Sbjct: 481 MVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASV 540

Query: 541 LQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 573
           LQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 541 LQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 579

BLAST of Lag0020158 vs. ExPASy TrEMBL
Match: A0A6J1EAL1 (GTPase-activating protein GYP7 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430845 PE=4 SV=1)

HSP 1 Score: 997.3 bits (2577), Expect = 2.7e-287
Identity = 525/575 (91.30%), Postives = 536/575 (93.22%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60
           MRALKRTPT SSPDPNSNSPPSSSS SSA SSASSSWVHLRSVLFVVTSSSPASSSSSDR
Sbjct: 1   MRALKRTPTSSSPDPNSNSPPSSSSPSSALSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60

Query: 61  GRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 120
           GRLKSPWSRKKRKHALSPQQWRSLFTPDG+LRDGGIKFLKKVRSGGVDPSIRAEVWPFLL
Sbjct: 61  GRLKSPWSRKKRKHALSPQQWRSLFTPDGRLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 120

Query: 121 GVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHVA 180
           G YDLSSSEEERDAVRVQKRKEYE+LRKQCRSLLKFGD  IKLNDDEMN NK  D Q V+
Sbjct: 121 GFYDLSSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDGCIKLNDDEMNFNKVGDAQLVS 180

Query: 181 HCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLEED 240
           H                   GDDSPSLEDV SARESISSDERG  FRYLDGTS VLLEED
Sbjct: 181 H-------------------GDDSPSLEDVGSARESISSDERGTNFRYLDGTSEVLLEED 240

Query: 241 DSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPLVTEVTS 300
           DSSR+MTNAD S+LNTESSDSDSSEDPEVSQTFPSSDGREDNDP FTSKNSSPLVTEVTS
Sbjct: 241 DSSRQMTNADVSILNTESSDSDSSEDPEVSQTFPSSDGREDNDPVFTSKNSSPLVTEVTS 300

Query: 301 KFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLDSCR 360
           KFR NEDFTTWQRIIRLDAVRANAEWIAYAPS AAVSDDKARHSAEVVGLKDYDHL+SCR
Sbjct: 301 KFRGNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDKARHSAEVVGLKDYDHLESCR 360

Query: 361 IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR 420
           IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR
Sbjct: 361 IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR 420

Query: 421 LDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLW 480
           LDEVGIRKQLN+VS+II+FKDSHLYRHLQDL+AEDCFFVYRMVVVLFRRELTFEQTLCLW
Sbjct: 421 LDEVGIRKQLNVVSKIIRFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQTLCLW 480

Query: 481 EVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERYNSMDEIIR 540
           EVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE+Y SMDEIIR
Sbjct: 481 EVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYYSMDEIIR 540

Query: 541 ECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 576
           ECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 541 ECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 556

BLAST of Lag0020158 vs. ExPASy TrEMBL
Match: A0A6J1J3G4 (GTPase-activating protein GYP7 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483051 PE=4 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 3.0e-286
Identity = 523/575 (90.96%), Postives = 537/575 (93.39%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60
           MRALKRTPT SSPDPNSNSPPSSSS SSA SSASSSWVHLRSVLFVVTSSSPASSSSSDR
Sbjct: 68  MRALKRTPTSSSPDPNSNSPPSSSSPSSALSSASSSWVHLRSVLFVVTSSSPASSSSSDR 127

Query: 61  GRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 120
           GRLKSPWSRKKRKHALSPQQWRSLFTPDG+LRDGGIKFLKKVRSGGVDPSIRAEVWPFLL
Sbjct: 128 GRLKSPWSRKKRKHALSPQQWRSLFTPDGRLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 187

Query: 121 GVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHVA 180
           G YDLSSSEEERDAVRVQKRKEYE+LRKQCRSLLKFGD  IKLNDDEMN NKE D Q V+
Sbjct: 188 GFYDLSSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDGCIKLNDDEMNFNKEGDAQLVS 247

Query: 181 HCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLEED 240
           H                   GDDSPSLEDV SARESISSDERG  FRYLDG+S VLLEED
Sbjct: 248 H-------------------GDDSPSLEDVGSARESISSDERGTNFRYLDGSSEVLLEED 307

Query: 241 DSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPLVTEVTS 300
           +SSR+MTNAD S+LNTESSDSDSSEDPEVSQTFPSSDGREDNDP FTSKNSSPLVTEVTS
Sbjct: 308 NSSRQMTNADVSVLNTESSDSDSSEDPEVSQTFPSSDGREDNDPVFTSKNSSPLVTEVTS 367

Query: 301 KFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLDSCR 360
           KFR NEDFTTWQRIIRLDAVRANAEWIAYAPS AAVSDDKAR+SAEVVGLKDYDHL+SCR
Sbjct: 368 KFRGNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDKARYSAEVVGLKDYDHLESCR 427

Query: 361 IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR 420
           IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR
Sbjct: 428 IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR 487

Query: 421 LDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLW 480
           LDEVGIRKQLN+VS+II+FKDSHLYRHLQDL+AEDCFFVYRMVVVLFRRELTFEQTLCLW
Sbjct: 488 LDEVGIRKQLNVVSKIIRFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQTLCLW 547

Query: 481 EVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERYNSMDEIIR 540
           EVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE+Y SMDEIIR
Sbjct: 548 EVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYYSMDEIIR 607

Query: 541 ECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 576
           ECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 608 ECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 623

BLAST of Lag0020158 vs. ExPASy TrEMBL
Match: A0A6J1J9X5 (GTPase-activating protein GYP7 isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC111483051 PE=4 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 1.1e-285
Identity = 522/574 (90.94%), Postives = 536/574 (93.38%), Query Frame = 0

Query: 2   RALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPASSSSSDRG 61
           RALKRTPT SSPDPNSNSPPSSSS SSA SSASSSWVHLRSVLFVVTSSSPASSSSSDRG
Sbjct: 52  RALKRTPTSSSPDPNSNSPPSSSSPSSALSSASSSWVHLRSVLFVVTSSSPASSSSSDRG 111

Query: 62  RLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 121
           RLKSPWSRKKRKHALSPQQWRSLFTPDG+LRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG
Sbjct: 112 RLKSPWSRKKRKHALSPQQWRSLFTPDGRLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 171

Query: 122 VYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHVAH 181
            YDLSSSEEERDAVRVQKRKEYE+LRKQCRSLLKFGD  IKLNDDEMN NKE D Q V+H
Sbjct: 172 FYDLSSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDGCIKLNDDEMNFNKEGDAQLVSH 231

Query: 182 CDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLEEDD 241
                              GDDSPSLEDV SARESISSDERG  FRYLDG+S VLLEED+
Sbjct: 232 -------------------GDDSPSLEDVGSARESISSDERGTNFRYLDGSSEVLLEEDN 291

Query: 242 SSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPLVTEVTSK 301
           SSR+MTNAD S+LNTESSDSDSSEDPEVSQTFPSSDGREDNDP FTSKNSSPLVTEVTSK
Sbjct: 292 SSRQMTNADVSVLNTESSDSDSSEDPEVSQTFPSSDGREDNDPVFTSKNSSPLVTEVTSK 351

Query: 302 FRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLDSCRI 361
           FR NEDFTTWQRIIRLDAVRANAEWIAYAPS AAVSDDKAR+SAEVVGLKDYDHL+SCRI
Sbjct: 352 FRGNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDKARYSAEVVGLKDYDHLESCRI 411

Query: 362 FHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRL 421
           FHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRL
Sbjct: 412 FHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRL 471

Query: 422 DEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWE 481
           DEVGIRKQLN+VS+II+FKDSHLYRHLQDL+AEDCFFVYRMVVVLFRRELTFEQTLCLWE
Sbjct: 472 DEVGIRKQLNVVSKIIRFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQTLCLWE 531

Query: 482 VMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERYNSMDEIIRE 541
           VMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE+Y SMDEIIRE
Sbjct: 532 VMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYYSMDEIIRE 591

Query: 542 CNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 576
           CNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 592 CNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 606

BLAST of Lag0020158 vs. TAIR 10
Match: AT3G49350.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 661.8 bits (1706), Expect = 5.1e-190
Identity = 368/576 (63.89%), Postives = 437/576 (75.87%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPASSSSSDR 60
           M+AL+R  T S     S+S PSS  SS  SSS+SSSW+ +RS LFVV SSSPA  S SDR
Sbjct: 1   MKALRRIQTTS-----SSSNPSSPLSSPPSSSSSSSWIQIRSALFVVASSSPA--SCSDR 60

Query: 61  GRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLL 120
            RLKSPWSR+KRK  L PQQW+  FTPDG+LR+GG+  LKKVRS G++PSIR EVWPFLL
Sbjct: 61  PRLKSPWSRRKRKRPLRPQQWKRFFTPDGRLRNGGVDLLKKVRSRGIEPSIRLEVWPFLL 120

Query: 121 GVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHVA 180
           G+Y  +SS+EER  +R ++RKEYE+LR+QC+ L K  + + KL +       E D   V 
Sbjct: 121 GLYGFNSSKEERVTIRNRRRKEYERLRRQCKRLQKHNNGTRKLYNGSETIQDEYDWPRVQ 180

Query: 181 HCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDER-GPGFRYLDGTSGVLLEE 240
             D S   C+                 ++VVSARES+SSDE       Y+   S  +  +
Sbjct: 181 DTDSS---CS-----------------DEVVSARESLSSDEDITEDIGYMSEVSCTVERD 240

Query: 241 D-DSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPLVTEV 300
           D  SSRR+TNA  S LN+ESSDSDSS++ EV Q F SS    D +  + + +S P     
Sbjct: 241 DGSSSRRITNATISTLNSESSDSDSSDESEVVQVFQSS-ATPDVNSTYPASSSIP----- 300

Query: 301 TSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLDS 360
               R+ EDF TWQRIIRLDAVRA++EW +Y+PSQA +S+D+A  +AE VGLKDY+HL+ 
Sbjct: 301 ----RTEEDFVTWQRIIRLDAVRADSEWTSYSPSQAVISEDRACRAAEAVGLKDYNHLEP 360

Query: 361 CRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHN 420
            +IF AARLV +LEAYALYDP+IGYCQGMSDLLSPI++VI +DHE FWCFVGFM+KARHN
Sbjct: 361 YKIFQAARLVAVLEAYALYDPDIGYCQGMSDLLSPILSVIPDDHEVFWCFVGFMKKARHN 420

Query: 421 FRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLC 480
           FRLDEVGIR+QLNIVS+IIK KDS LYRHL+ LQAEDCFFVYRMVVV+FRRELT +QTLC
Sbjct: 421 FRLDEVGIRRQLNIVSKIIKSKDSQLYRHLEKLQAEDCFFVYRMVVVMFRRELTLDQTLC 480

Query: 481 LWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIERYNSMDEI 540
           LWEVMWADQAAIRAG+GKSAWSRIRQRAPPT+DL+LYAIAASVLQ+RK IIERYNSMDEI
Sbjct: 481 LWEVMWADQAAIRAGMGKSAWSRIRQRAPPTDDLVLYAIAASVLQRRKRIIERYNSMDEI 539

Query: 541 IRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFN 575
           +REC SMAGQLDVWKLLDDAHDLVVTLH KIE SF+
Sbjct: 541 LRECQSMAGQLDVWKLLDDAHDLVVTLHTKIEHSFS 539

BLAST of Lag0020158 vs. TAIR 10
Match: AT5G53570.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 625.2 bits (1611), Expect = 5.3e-179
Identity = 356/581 (61.27%), Postives = 431/581 (74.18%), Query Frame = 0

Query: 3   ALKRTPTPSSPDPNSNSPPSSSS---SSSASSSASSSWVHLRSVLFVVTSSSPASSSSSD 62
           +L  + + S P  +S+SPPSS+S   S+S SSS+SSSW+HLRSVLFV   SSP+S +SSD
Sbjct: 21  SLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFVANLSSPSSVTSSD 80

Query: 63  RGRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 122
           R R KSPWSR+KRK AL+P QWRSLFTP+GKLRDGG+ FLKKVRS GVDPSIRAEVW FL
Sbjct: 81  R-RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFL 140

Query: 123 LGVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHV 182
           LGVYDL+S+ EER+AV+ QKRKEYEKL+++C+ LLK G             N   DN   
Sbjct: 141 LGVYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCG-------------NGSTDNLEE 200

Query: 183 AHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLEE 242
              D++   C +  ++  +     S   +DVVSA                          
Sbjct: 201 LPSDEANSQCVRFVDDYKITGPMTS---QDVVSA-------------------------- 260

Query: 243 DDSSRRMTNADASLLNTESSDSDSSEDPE---VSQTFPSSDGR---EDNDPDFTSKNSSP 302
                         LNT+SSD+DS ED E   +  +F  SD +   EDN  + + +NSS 
Sbjct: 261 --------------LNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSSL 320

Query: 303 LV---TEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGL 362
           LV   +EV  +   +EDF+TWQRIIRLDA+RA++EW  Y+P   A+++ KAR  AE VGL
Sbjct: 321 LVAAASEVQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITESKARRLAESVGL 380

Query: 363 KDYDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVG 422
           KDYDHL+SCR++HAARLV ILEAYA+YDPEIGYCQGMSDLLSPI+ VI+EDHEAFWCFVG
Sbjct: 381 KDYDHLESCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFVG 440

Query: 423 FMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRE 482
           FM+KARHNFRLDE GI++QL+IVS+IIK KDS LY+HL++LQAEDC FVYRMV+V+FRRE
Sbjct: 441 FMKKARHNFRLDEAGIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRRE 500

Query: 483 LTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE 542
           L+FEQTLCLWEVMWADQAAIRAGVGKS WSRIRQ+APPT+DLLLYAIAA VL +RKLII+
Sbjct: 501 LSFEQTLCLWEVMWADQAAIRAGVGKSPWSRIRQQAPPTDDLLLYAIAALVL-RRKLIIQ 543

Query: 543 RYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIET 572
           +Y+SMDEI+ ECNSMAGQL+VWKLLDDAH LVVTLH+KIET
Sbjct: 561 KYSSMDEIVEECNSMAGQLNVWKLLDDAHHLVVTLHDKIET 543

BLAST of Lag0020158 vs. TAIR 10
Match: AT5G53570.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 625.2 bits (1611), Expect = 5.3e-179
Identity = 356/581 (61.27%), Postives = 431/581 (74.18%), Query Frame = 0

Query: 3   ALKRTPTPSSPDPNSNSPPSSSS---SSSASSSASSSWVHLRSVLFVVTSSSPASSSSSD 62
           +L  + + S P  +S+SPPSS+S   S+S SSS+SSSW+HLRSVLFV   SSP+S +SSD
Sbjct: 48  SLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFVANLSSPSSVTSSD 107

Query: 63  RGRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 122
           R R KSPWSR+KRK AL+P QWRSLFTP+GKLRDGG+ FLKKVRS GVDPSIRAEVW FL
Sbjct: 108 R-RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFL 167

Query: 123 LGVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNKERDNQHV 182
           LGVYDL+S+ EER+AV+ QKRKEYEKL+++C+ LLK G             N   DN   
Sbjct: 168 LGVYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCG-------------NGSTDNLEE 227

Query: 183 AHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDERGPGFRYLDGTSGVLLEE 242
              D++   C +  ++  +     S   +DVVSA                          
Sbjct: 228 LPSDEANSQCVRFVDDYKITGPMTS---QDVVSA-------------------------- 287

Query: 243 DDSSRRMTNADASLLNTESSDSDSSEDPE---VSQTFPSSDGR---EDNDPDFTSKNSSP 302
                         LNT+SSD+DS ED E   +  +F  SD +   EDN  + + +NSS 
Sbjct: 288 --------------LNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSSL 347

Query: 303 LV---TEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGL 362
           LV   +EV  +   +EDF+TWQRIIRLDA+RA++EW  Y+P   A+++ KAR  AE VGL
Sbjct: 348 LVAAASEVQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITESKARRLAESVGL 407

Query: 363 KDYDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVG 422
           KDYDHL+SCR++HAARLV ILEAYA+YDPEIGYCQGMSDLLSPI+ VI+EDHEAFWCFVG
Sbjct: 408 KDYDHLESCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFVG 467

Query: 423 FMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRE 482
           FM+KARHNFRLDE GI++QL+IVS+IIK KDS LY+HL++LQAEDC FVYRMV+V+FRRE
Sbjct: 468 FMKKARHNFRLDEAGIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRRE 527

Query: 483 LTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIE 542
           L+FEQTLCLWEVMWADQAAIRAGVGKS WSRIRQ+APPT+DLLLYAIAA VL +RKLII+
Sbjct: 528 LSFEQTLCLWEVMWADQAAIRAGVGKSPWSRIRQQAPPTDDLLLYAIAALVL-RRKLIIQ 570

Query: 543 RYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIET 572
           +Y+SMDEI+ ECNSMAGQL+VWKLLDDAH LVVTLH+KIET
Sbjct: 588 KYSSMDEIVEECNSMAGQLNVWKLLDDAHHLVVTLHDKIET 570

BLAST of Lag0020158 vs. TAIR 10
Match: AT5G24390.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 587.8 bits (1514), Expect = 9.4e-168
Identity = 329/576 (57.12%), Postives = 419/576 (72.74%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSASSSWVHLRSVLFVVTSSSPAS-SSSSD 60
           M+AL+R  T S+  PN +SP             SS W HLRS   +VTSSSPAS SSSSD
Sbjct: 1   MKALRRIQTKST-FPNPSSP-------------SSPWTHLRSAFVLVTSSSPASCSSSSD 60

Query: 61  RGRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 120
             RLKSPWSR+K K  L+ ++WR  FTP+G++R+GG+  LKKVR+ G+DPSIR+EVWPFL
Sbjct: 61  PHRLKSPWSRRKGKKPLTLRRWRRCFTPEGRIRNGGVDLLKKVRTRGIDPSIRSEVWPFL 120

Query: 121 LGVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESIKLNDDEMNCNK-ERDNQH 180
           LGV D +SSEEER A R  +RK YE+LR+QC+ L K    + KLN      NK  +D++H
Sbjct: 121 LGVCDFNSSEEERGATRTWRRKVYERLRRQCKRLQKQNSGTFKLN----KINKTTQDDEH 180

Query: 181 VAHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSD-ERGPGFRYLDGTSGVLL 240
                          ++  +A   +S   +D  S  ES+ SD +      Y+   S  L 
Sbjct: 181 ---------------DSWSLAQDSESSCSDDACSVHESLISDKDNTEDIGYMSDVSCTLD 240

Query: 241 EEDDSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPLVTE 300
            + + SR         LN+ESSDSDSS++ +  Q  PS +G+++N       ++SP    
Sbjct: 241 RDYNGSRH--------LNSESSDSDSSDENDYVQVAPSCEGKDEN-------STSPSSIY 300

Query: 301 VTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLD 360
           ++   R+ EDF TWQRIIRLDA+RA+ EW  Y+ SQA VS+++AR +AE VGLKDY +L+
Sbjct: 301 IS---RTKEDFVTWQRIIRLDALRADTEWTPYSQSQALVSENRARRAAEAVGLKDYSYLE 360

Query: 361 SCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARH 420
             +IFHAARLV +LEAYAL+DPEIGYCQGMSDLLSPI++VI +D+EAFWCFVGFM+KAR 
Sbjct: 361 PSKIFHAARLVAVLEAYALHDPEIGYCQGMSDLLSPILSVIPDDYEAFWCFVGFMKKARQ 420

Query: 421 NFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTL 480
           NFR+DEVGI +QLNIVS+IIK KDS LY+HL+ ++AEDCFFVYRMV+V+FRRELT EQTL
Sbjct: 421 NFRVDEVGITRQLNIVSKIIKSKDSQLYKHLEKVKAEDCFFVYRMVLVMFRRELTLEQTL 480

Query: 481 CLWEVMWADQAAIRAGVGKSAWS-RIRQRAPPTEDLLLYAIAASVLQKRKLIIERYNSMD 540
            LWEV+WADQAAIRAG+GKS+WS RI+QRAPPTEDLLLY +AASVLQ+RK+IIE+Y+SM+
Sbjct: 481 HLWEVIWADQAAIRAGMGKSSWSRRIKQRAPPTEDLLLYVVAASVLQRRKVIIEKYSSME 525

Query: 541 EIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 573
           EI+REC++M G+LDVWKLLDDAHDL+VTLH KIE S
Sbjct: 541 EILRECHNMVGKLDVWKLLDDAHDLIVTLHAKIEHS 525

BLAST of Lag0020158 vs. TAIR 10
Match: AT5G41940.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 554.7 bits (1428), Expect = 8.9e-158
Identity = 305/529 (57.66%), Postives = 379/529 (71.64%), Query Frame = 0

Query: 42  SVLFVVTSSSPASSSSSDRGRLKSPWSRKKRKHALSPQQWRSLFTPDGKLRDGGIKFLKK 101
           +V   V + + A +  S RG + SPWS ++RK  L P+QW + FT +G+L DGG+KFLKK
Sbjct: 55  AVAVTVMAVAVAFTVYSRRGSIGSPWSLRRRKRVLQPKQWNAFFTEEGRLSDGGVKFLKK 114

Query: 102 VRSGGVDPSIRAEVWPFLLGVYDLSSSEEERDAVRVQKRKEYEKLRKQCRSLLKFGDESI 161
           VRSGGV PSIR EVWPFLLGVYDL S++EERD++R  K  EYE LR+QCR          
Sbjct: 115 VRSGGVHPSIRPEVWPFLLGVYDLKSNKEERDSIRQLKLTEYENLRRQCR---------- 174

Query: 162 KLNDDEMNCNKERDNQHVAHCDDSPMNCNKEGNNQHVADGDDSPSLEDVVSARESISSDE 221
           ++++   N    ++    ++ +DS +               +S  +E+V S+  +I+ DE
Sbjct: 175 EIHERYENVCDSKETAQSSNTEDSQVL--------------ESHDIEEVESSTRTITVDE 234

Query: 222 RGPGFRYLDGTSGVLLEEDDSSRRMTNADASLLNTESSDSDSSEDPEVSQTFPSSDGRED 281
                  L+  S ++ +E+     +T  DA+      SDS + E+ E S    + +    
Sbjct: 235 S----EKLNSES-IMQDENCEKSDVTTEDAA---GNDSDSTNPEETETSPFLANEEVESH 294

Query: 282 NDPDFTSKNSSPLVTEVTSKFRSNEDF-TTWQRIIRLDAVRANAEWIAYAPSQAAVSDDK 341
           N  +   + SSP  +       ++EDF +TWQRIIRLDAVRAN EW+ Y+P+QAAVS+ K
Sbjct: 295 NTVNQEKEISSP--SSKPKSQAADEDFMSTWQRIIRLDAVRANDEWVPYSPTQAAVSETK 354

Query: 342 ARHSAEVVGLKDYDHLDSCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITE 401
           AR  A  VGL DYDHL+ CRIFHAARLV ILEAYA+YDPEIGYCQGMSDLLSP+I V+ +
Sbjct: 355 ARGIAIQVGLNDYDHLEPCRIFHAARLVGILEAYAVYDPEIGYCQGMSDLLSPLIAVMED 414

Query: 402 DHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVY 461
           D  AFWCFVGFM KARHNFRLDEVGIR+QL++VS+IIKFKD HLYRHL++L+AEDCFFVY
Sbjct: 415 DVLAFWCFVGFMSKARHNFRLDEVGIRRQLSMVSKIIKFKDIHLYRHLENLEAEDCFFVY 474

Query: 462 RMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAAS 521
           RMVVVLFRRELTFEQTLCLWEVMWADQAAIR G+ K+ W RIR RAPPTEDLLLYAIAAS
Sbjct: 475 RMVVVLFRRELTFEQTLCLWEVMWADQAAIRTGIAKATWGRIRLRAPPTEDLLLYAIAAS 534

Query: 522 VLQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKI 570
           VLQ+RK IIE+Y+ MDEI++ECNSMAG LDVWKLLDDAHDLVV LH+KI
Sbjct: 535 VLQRRKTIIEKYSGMDEIMKECNSMAGHLDVWKLLDDAHDLVVNLHDKI 549

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141059.11.3e-28890.91GTPase-activating protein GYP7-like isoform X1 [Momordica charantia][more]
XP_022968472.13.3e-28789.68small G protein signaling modulator 2-like isoform X3 [Cucurbita maxima][more]
XP_022923060.15.5e-28791.30GTPase-activating protein GYP7 isoform X1 [Cucurbita moschata][more]
XP_038896358.13.6e-28691.02GTPase-activating protein GYP7-like isoform X1 [Benincasa hispida][more]
KAG7013330.13.6e-28689.34GYP7 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q94BY97.5e-17861.27Rab GTPase-activating protein 22 OS=Arabidopsis thaliana OX=3702 GN=RABGAP22 PE=... [more]
P093791.3e-2530.29GTPase-activating protein GYP7 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) ... [more]
Q6FWI16.2e-2322.85GTPase-activating protein GYP7 OS=Candida glabrata (strain ATCC 2001 / CBS 138 /... [more]
P483651.1e-2228.03GTPase-activating protein GYP7 OS=Saccharomyces cerevisiae (strain ATCC 204508 /... [more]
Q8TC071.4e-2228.77TBC1 domain family member 15 OS=Homo sapiens OX=9606 GN=TBC1D15 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CHH56.4e-28990.91GTPase-activating protein GYP7-like isoform X1 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1HTL11.6e-28789.68small G protein signaling modulator 2-like isoform X3 OS=Cucurbita maxima OX=366... [more]
A0A6J1EAL12.7e-28791.30GTPase-activating protein GYP7 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1J3G43.0e-28690.96GTPase-activating protein GYP7 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1J9X51.1e-28590.94GTPase-activating protein GYP7 isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G49350.15.1e-19063.89Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G53570.15.3e-17961.27Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G53570.25.3e-17961.27Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G24390.19.4e-16857.12Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G41940.18.9e-15857.66Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000195Rab-GTPase-TBC domainSMARTSM00164tbc_4coord: 103..532
e-value: 2.8E-30
score: 116.6
IPR000195Rab-GTPase-TBC domainPFAMPF00566RabGAP-TBCcoord: 366..487
e-value: 1.0E-31
score: 110.3
IPR000195Rab-GTPase-TBC domainPROSITEPS50086TBC_RABGAPcoord: 106..487
score: 31.647995
NoneNo IPR availableGENE3D1.10.8.270putative rabgap domain of human tbc1 domain family member 14 like domainscoord: 289..419
e-value: 3.8E-18
score: 67.5
NoneNo IPR availableGENE3D1.10.472.80coord: 423..570
e-value: 9.4E-22
score: 79.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..81
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..277
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 178..294
NoneNo IPR availablePANTHERPTHR22957TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEINcoord: 10..569
NoneNo IPR availablePANTHERPTHR22957:SF585SMALL G PROTEIN SIGNALING MODULATOR 2-LIKEcoord: 10..569
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 424..561
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 89..447

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020158.1Lag0020158.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090630 activation of GTPase activity
biological_process GO:0006886 intracellular protein transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005096 GTPase activator activity