Lag0020126 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020126
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr5: 48312599 .. 48315080 (-)
RNA-Seq ExpressionLag0020126
SyntenyLag0020126
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGGATAGTATTGAGTGTGTATCGTCTTCAGATGGTCTCGATGAGGATGAGATCCATCTGCATCACACTCTTCATCCTTATTCCCATTCTCATCTTCATCCAGAGTTGTCAGCAACGAAGCCTCGTAATGGAAACAACAATAACGCTATTACAGGCCCCACTGCCACTGCACCGGCCACTAGCGTCCATGAATTGCTTGAGTGCCCTGTCTGCACCAATTCTATGTATCCTCCCATCCATCAGGTTTGTCTTTGTTTCCCTAATAATTACTTACAGAATAGAGATTGGGGGGAGGGGGTGGCTTTCTTCATATTTGGATTTGATTTTCAATTTACTAGTTTTTTTCGACATAATTTCCTTTGATTTATCTTTTCCTTGCTGGGTTTTTGATTTAAGGTTGATCATTCAATGTCGATTGGAAGTTTGGGGGTGCGAAGTGTTTTGGATGGATATGTTAACTGGGAACTTATTTAAAAGTCATGAACTGTGATATATAAAATCTAGAACTAGACTATATCGAAATATGCCAAGTTGTTGTATCATATGGTAACCATTAAGCATAAACAGCTCTCTTACTTCTTAAGGTTTTCTAACACTAGACTGTATAATTGAGATGAGATTATTACTTGTCCTATTTGTGCTTGAATGTCTTGGTTTCTAGCTTAAGATGCTTGGTTGTTTCAACCTACTTTCTAAAGGGACTTTTTGGGTTTATTCATGGCATGATTTTGACTGGTTGATGAAACTTTAGTTTCCCATTGCTTTTTTAGAATATCATTTCCCATTATTGCAGTTATCTTTGGGTTTCTTCTTGTATAATTTGGTAGCATGTGTACCATCAGGACCAATATTGTCTTAAAACATGTTAGGAGATATATGTTTTGTTGTTGATATATATCCAATGCTTGTATGTAAATGTGGGTTAACTGTATAATCAACTAAATGTTGCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGTTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCGCTTGAGAAGGTGGCAGAGTCACTGGAATTGCCTTGCAAGTATTATTCCTTGGGGTGCCCTGAGATATTTCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTTCAGACCCTACAATTGCCCATATGCTGGGTCGGAATGCTCTGCTGTTGGAGATATTCCTTTCCTTGTTGCCCATCTGAGGGATGATCACAAGGTGGACATGCACACTGGATGCACATTTAACCATCGCTATGTGAAATCCAATCCAAGGGAAGTAGAAAATGCCACTTGGATGCTTACTGTAAGTCTAATGTATTTGATCTTAATTCTTAAGTGATTCTCTTTTTCTTCTTTGGTAGAAATTGCTTTATCATACACGAACACCATTCCTTACATGTGATATATATACAACCACGTGAGCACGTGACATGTGTGTCTTCGTGTGTGTGAGGGCAATGATTACAAACCCAATCAAGCACTAGGGAGGTTAGAAAAAGCTTCCTACTAGTCAATAACAATATATGTATATGATGAAACAAAATCATTGGTATTATATGTAGCCTGCTACAAGTTGTAAGTTTTATAAACCATTAAAGATGGTTCATAAGTTTTTCTGATGGTGTGAAGAAGCCGAAGGTATTGTTGCAAGTCGTTGACATGTTGGGTAATCAATCATTTTCTTTTAAGTTCTATTTTATAAGGCTAGTGACCTTAACTGCCTTAACTTTTTGCCTTTTTCTAGTGGCAAATGGATGTGATGCAATAAAATAAAAGTTTGGTTGAGATATCAAGGGATCAAGAGAACGCTTTTTATTTAATATCTTCAATCTCCTTTTTCTTCAAGTGAAGGATTTATGCTCTCTGCTGTCGGTTGAGTTTTTTCATTAAGTTTATTGCCTCATCCATACACTTGAAAGATTGGTCAATGGATGCTGAACTACCAATAAACGGCCTATTTTGTGTTTAATCAAATGACTTTGACTTTACGCTGGAGTGAGACATTCTGCTGGACATCTCTTGGGTTTACTCCAAATTTTGGTTTGATCCTGTTTCTTGTGGCTTGCTCTCACATTTTGCAGGTTTTCCATTGTTTTGGTCAATATTTCTGCCTTCACTTTGAAGCCTTTCAGCTAGGCATGGCCCCAGTCTATATGGCTTTCCTTCGTTTCATGGGTGATGAGAATGAGGCGCGAACCTACAGCTACAGTCTCGAGGTGGGTGGATACGGGCGAAAACTAATATGGGAGGGCACCCCACGAAGTATTAGAGATAGTCATCGAAAAGTGAGGGACAGCCATGATGGACTTATCATTCAAAGAAATATGGCTCTTTTCTTCTCTGGTGGGGATAGGAAGGAACTGAAGCTGAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCTGATGCCGGGGTCTGCATACCGAACCTGTGTAGCTAA

mRNA sequence

ATGGATTTGGATAGTATTGAGTGTGTATCGTCTTCAGATGGTCTCGATGAGGATGAGATCCATCTGCATCACACTCTTCATCCTTATTCCCATTCTCATCTTCATCCAGAGTTGTCAGCAACGAAGCCTCGTAATGGAAACAACAATAACGCTATTACAGGCCCCACTGCCACTGCACCGGCCACTAGCGTCCATGAATTGCTTGAGTGCCCTGTCTGCACCAATTCTATGTATCCTCCCATCCATCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGTTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCGCTTGAGAAGGTGGCAGAGTCACTGGAATTGCCTTGCAAGTATTATTCCTTGGGGTGCCCTGAGATATTTCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTTCAGACCCTACAATTGCCCATATGCTGGGTCGGAATGCTCTGCTGTTGGAGATATTCCTTTCCTTGTTGCCCATCTGAGGGATGATCACAAGGTGGACATGCACACTGGATGCACATTTAACCATCGCTATGTGAAATCCAATCCAAGGGAAGTAGAAAATGCCACTTGGATGCTTACTGTTTTCCATTGTTTTGGTCAATATTTCTGCCTTCACTTTGAAGCCTTTCAGCTAGGCATGGCCCCAGTCTATATGGCTTTCCTTCGTTTCATGGGTGATGAGAATGAGGCGCGAACCTACAGCTACAGTCTCGAGGTGGGTGGATACGGGCGAAAACTAATATGGGAGGGCACCCCACGAAGTATTAGAGATAGTCATCGAAAAGTGAGGGACAGCCATGATGGACTTATCATTCAAAGAAATATGGCTCTTTTCTTCTCTGGTGGGGATAGGAAGGAACTGAAGCTGAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCTGATGCCGGGGTCTGCATACCGAACCTGTGTAGCTAA

Coding sequence (CDS)

ATGGATTTGGATAGTATTGAGTGTGTATCGTCTTCAGATGGTCTCGATGAGGATGAGATCCATCTGCATCACACTCTTCATCCTTATTCCCATTCTCATCTTCATCCAGAGTTGTCAGCAACGAAGCCTCGTAATGGAAACAACAATAACGCTATTACAGGCCCCACTGCCACTGCACCGGCCACTAGCGTCCATGAATTGCTTGAGTGCCCTGTCTGCACCAATTCTATGTATCCTCCCATCCATCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGTTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCGCTTGAGAAGGTGGCAGAGTCACTGGAATTGCCTTGCAAGTATTATTCCTTGGGGTGCCCTGAGATATTTCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTTCAGACCCTACAATTGCCCATATGCTGGGTCGGAATGCTCTGCTGTTGGAGATATTCCTTTCCTTGTTGCCCATCTGAGGGATGATCACAAGGTGGACATGCACACTGGATGCACATTTAACCATCGCTATGTGAAATCCAATCCAAGGGAAGTAGAAAATGCCACTTGGATGCTTACTGTTTTCCATTGTTTTGGTCAATATTTCTGCCTTCACTTTGAAGCCTTTCAGCTAGGCATGGCCCCAGTCTATATGGCTTTCCTTCGTTTCATGGGTGATGAGAATGAGGCGCGAACCTACAGCTACAGTCTCGAGGTGGGTGGATACGGGCGAAAACTAATATGGGAGGGCACCCCACGAAGTATTAGAGATAGTCATCGAAAAGTGAGGGACAGCCATGATGGACTTATCATTCAAAGAAATATGGCTCTTTTCTTCTCTGGTGGGGATAGGAAGGAACTGAAGCTGAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCTGATGCCGGGGTCTGCATACCGAACCTGTGTAGCTAA

Protein sequence

MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Homology
BLAST of Lag0020126 vs. NCBI nr
Match: XP_022141336.1 (E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia])

HSP 1 Score: 707.6 bits (1825), Expect = 5.1e-200
Identity = 327/331 (98.79%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSH HPELSA+KPRNG+NN+AITGPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. NCBI nr
Match: XP_022984623.1 (E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 705.3 bits (1819), Expect = 2.5e-199
Identity = 326/331 (98.49%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSH HPELS+TKPRNG+NN+AITGPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. NCBI nr
Match: XP_022922771.1 (E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita moschata] >XP_023553000.1 E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita pepo subsp. pepo] >KAG7014703.1 E3 ubiquitin-protein ligase SINAT4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 703.7 bits (1815), Expect = 7.3e-199
Identity = 325/331 (98.19%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSH HPELS+TKPRNG+NN+AITGPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. NCBI nr
Match: XP_022945143.1 (E3 ubiquitin-protein ligase SINAT5-like [Cucurbita moschata] >XP_023541986.1 E3 ubiquitin-protein ligase SINAT5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 702.2 bits (1811), Expect = 2.1e-198
Identity = 323/331 (97.58%), Postives = 328/331 (99.09%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLH YSHSH HPELSATKPRNGNNN+A+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. NCBI nr
Match: XP_022968641.1 (E3 ubiquitin-protein ligase SINAT5-like [Cucurbita maxima])

HSP 1 Score: 700.7 bits (1807), Expect = 6.2e-198
Identity = 322/331 (97.28%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDG DEDEIHLHHTLH YSHSH HPELSATKPRNGNNN+A+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGFDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 4.8e-161
Identity = 268/333 (80.48%), Postives = 282/333 (84.68%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDS++C S+ D  D++EIH           H +  +S     N N  N     +   P
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIH--------QDRHSYASVSKHHHTNNNTTNVNAAASGLLP 60

Query: 61  -ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEK 120
             TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLALEK
Sbjct: 61  TTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEK 120

Query: 121 VAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLR 180
           VAESLELPCK+ SLGCPEIFPYYSKLKHE +CNFRPY+CPYAGSECS  GDIPFLVAHLR
Sbjct: 121 VAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLR 180

Query: 181 DDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAF 240
           DDHKVDMH+GCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAF
Sbjct: 181 DDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAF 240

Query: 241 LRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFS 300
           LRFMGDE EAR Y+YSLEVGGYGRKLIWEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFS
Sbjct: 241 LRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFS 300

Query: 301 GGDRKELKLRVTGRIWKEQNPD---AGVCIPNL 330
           GGDRKELKLRVTGRIWKEQ       G CIPNL
Sbjct: 301 GGDRKELKLRVTGRIWKEQQQSGEGGGACIPNL 325

BLAST of Lag0020126 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 9.1e-160
Identity = 270/336 (80.36%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNA----ITGPT 60
           M+ DS+ECVSS+           + +H   + H   + S+TK   G    A    I GPT
Sbjct: 1   METDSMECVSSTG----------NEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPT 60

Query: 61  ATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLA 120
           ATAPATSV+ELLECPVCT SMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLA
Sbjct: 61  ATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLA 120

Query: 121 LEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVA 180
           LEKVAESLELPCK+Y+LGCPEIFPYYSKLKHE+LCNFRPY+CPYAGSEC  VGDIPFLVA
Sbjct: 121 LEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVA 180

Query: 181 HLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVY 240
           HLRDDHKVDMH G TFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGM PVY
Sbjct: 181 HLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVY 240

Query: 241 MAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMAL 300
           MAFLRFMGDE +AR+YSYSLEVGG GRKL WEGTPRSIRDSHRKVRDS+DGLIIQRNMAL
Sbjct: 241 MAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMAL 300

Query: 301 FFSGGDRKELKLRVTGRIWKEQ-NPDAGVCIPNLCS 332
           FFSGGDRKELKLRVTG+IWKEQ +PD+G+ IPNL S
Sbjct: 301 FFSGGDRKELKLRVTGKIWKEQHSPDSGLSIPNLSS 326

BLAST of Lag0020126 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-156
Identity = 263/332 (79.22%), Postives = 285/332 (85.84%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           M+ DSI+ V     +D+DEIH  H            + S+TK + G            +P
Sbjct: 1   METDSIDSV-----IDDDEIHQKH------------QFSSTKSQGG-------ATVVISP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSV+ELLECPVCTNSMYPPIHQCHNGHTLCSTCK+RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYY+LGC  IFPYYSKLKHE+ CNFRPY+CPYAGSEC+AVGDI FLVAHLRD
Sbjct: 121 AESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE++AR Y+YSLEVGG GRK  WEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKE-QNPDAGVCIPNLCS 332
           GD+KELKLRVTGRIWKE QNPD+GVCI ++CS
Sbjct: 301 GDKKELKLRVTGRIWKEQQNPDSGVCITSMCS 308

BLAST of Lag0020126 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 6.5e-134
Identity = 214/258 (82.95%), Postives = 234/258 (90.70%), Query Frame = 0

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ++SV ELLECPVC ++MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKV
Sbjct: 44  SSSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 103

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           A SLELPCKY + GC  I+PYY KLKHE+ C +RPY+CPYAGSEC+  GDIP+LV HL+D
Sbjct: 104 AASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKD 163

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH GCTFNHRYVKSNP EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFL
Sbjct: 164 DHKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFL 223

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGD+ EA+ YSYSLEVGG GRK+IW+G PRSIRDSHRKVRDS+DGLIIQRNMALFFSG
Sbjct: 224 RFMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSG 283

Query: 301 GDRKELKLRVTGRIWKEQ 319
           G+RKELKLRVTGRIWKEQ
Sbjct: 284 GERKELKLRVTGRIWKEQ 301

BLAST of Lag0020126 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 1.8e-131
Identity = 217/278 (78.06%), Postives = 235/278 (84.53%), Query Frame = 0

Query: 48  NNNAITGPTATA-------PATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHN 107
           NNN  T P +             V+ELLECPVCTN MYPPIHQC NGHTLCS CK RV N
Sbjct: 31  NNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQN 90

Query: 108 RCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPY 167
            CPTCR ELG+IRCLALEKVAESLE+PC+Y +LGC +IFPYYSKLKHE  C FRPY CPY
Sbjct: 91  TCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPY 150

Query: 168 AGSECSAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQY 227
           AGSECS  GDIP LV HL+DDHKVDMH GCTFNHRYVKSNP EVENATWMLTVF+CFG+ 
Sbjct: 151 AGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQ 210

Query: 228 FCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRK 287
           FCLHFEAFQLGMAPVYMAFLRFMGDENEA+ +SYSLEVG +GRKL W+G PRSIRDSHRK
Sbjct: 211 FCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRK 270

Query: 288 VRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 319
           VRDS DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 271 VRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of Lag0020126 vs. ExPASy TrEMBL
Match: A0A6J1CIV8 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111011754 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 2.4e-200
Identity = 327/331 (98.79%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSH HPELSA+KPRNG+NN+AITGPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. ExPASy TrEMBL
Match: A0A6J1JB27 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111482855 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.2e-199
Identity = 326/331 (98.49%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSH HPELS+TKPRNG+NN+AITGPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. ExPASy TrEMBL
Match: A0A6J1E513 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111430668 PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 3.5e-199
Identity = 325/331 (98.19%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSH HPELS+TKPRNG+NN+AITGPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. ExPASy TrEMBL
Match: A0A6J1FZZ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449471 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.0e-198
Identity = 323/331 (97.58%), Postives = 328/331 (99.09%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLH YSHSH HPELSATKPRNGNNN+A+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. ExPASy TrEMBL
Match: A0A6J1HVG5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467802 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 3.0e-198
Identity = 322/331 (97.28%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDSIECVSSSDG DEDEIHLHHTLH YSHSH HPELSATKPRNGNNN+A+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGFDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Lag0020126 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 568.5 bits (1464), Expect = 3.4e-162
Identity = 268/333 (80.48%), Postives = 282/333 (84.68%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNAITGPTATAP 60
           MDLDS++C S+ D  D++EIH           H +  +S     N N  N     +   P
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIH--------QDRHSYASVSKHHHTNNNTTNVNAAASGLLP 60

Query: 61  -ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEK 120
             TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLALEK
Sbjct: 61  TTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEK 120

Query: 121 VAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLR 180
           VAESLELPCK+ SLGCPEIFPYYSKLKHE +CNFRPY+CPYAGSECS  GDIPFLVAHLR
Sbjct: 121 VAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLR 180

Query: 181 DDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAF 240
           DDHKVDMH+GCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAF
Sbjct: 181 DDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAF 240

Query: 241 LRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFS 300
           LRFMGDE EAR Y+YSLEVGGYGRKLIWEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFS
Sbjct: 241 LRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFS 300

Query: 301 GGDRKELKLRVTGRIWKEQNPD---AGVCIPNL 330
           GGDRKELKLRVTGRIWKEQ       G CIPNL
Sbjct: 301 GGDRKELKLRVTGRIWKEQQQSGEGGGACIPNL 325

BLAST of Lag0020126 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 564.3 bits (1453), Expect = 6.4e-161
Identity = 270/336 (80.36%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHLHPELSATKPRNGNNNNA----ITGPT 60
           M+ DS+ECVSS+           + +H   + H   + S+TK   G    A    I GPT
Sbjct: 1   METDSMECVSSTG----------NEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPT 60

Query: 61  ATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLA 120
           ATAPATSV+ELLECPVCT SMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLA
Sbjct: 61  ATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLA 120

Query: 121 LEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVA 180
           LEKVAESLELPCK+Y+LGCPEIFPYYSKLKHE+LCNFRPY+CPYAGSEC  VGDIPFLVA
Sbjct: 121 LEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVA 180

Query: 181 HLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVY 240
           HLRDDHKVDMH G TFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGM PVY
Sbjct: 181 HLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVY 240

Query: 241 MAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMAL 300
           MAFLRFMGDE +AR+YSYSLEVGG GRKL WEGTPRSIRDSHRKVRDS+DGLIIQRNMAL
Sbjct: 241 MAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMAL 300

Query: 301 FFSGGDRKELKLRVTGRIWKEQ-NPDAGVCIPNLCS 332
           FFSGGDRKELKLRVTG+IWKEQ +PD+G+ IPNL S
Sbjct: 301 FFSGGDRKELKLRVTGKIWKEQHSPDSGLSIPNLSS 326

BLAST of Lag0020126 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 470.3 bits (1209), Expect = 1.3e-132
Identity = 217/278 (78.06%), Postives = 235/278 (84.53%), Query Frame = 0

Query: 48  NNNAITGPTATA-------PATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHN 107
           NNN  T P +             V+ELLECPVCTN MYPPIHQC NGHTLCS CK RV N
Sbjct: 31  NNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQN 90

Query: 108 RCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPY 167
            CPTCR ELG+IRCLALEKVAESLE+PC+Y +LGC +IFPYYSKLKHE  C FRPY CPY
Sbjct: 91  TCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPY 150

Query: 168 AGSECSAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQY 227
           AGSECS  GDIP LV HL+DDHKVDMH GCTFNHRYVKSNP EVENATWMLTVF+CFG+ 
Sbjct: 151 AGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQ 210

Query: 228 FCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRK 287
           FCLHFEAFQLGMAPVYMAFLRFMGDENEA+ +SYSLEVG +GRKL W+G PRSIRDSHRK
Sbjct: 211 FCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRK 270

Query: 288 VRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 319
           VRDS DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 271 VRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of Lag0020126 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 463.4 bits (1191), Expect = 1.5e-130
Identity = 208/258 (80.62%), Postives = 229/258 (88.76%), Query Frame = 0

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           +  V+ELLECPVCTN MYPPIHQC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKV
Sbjct: 48  SNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKV 107

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180
           AESLE+PC+Y +LGC +IFPYYSKLKHE  C FR Y+CPYAGSECS  GDIP LV HL+D
Sbjct: 108 AESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKD 167

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH GCTFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVYMAFL
Sbjct: 168 DHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFL 227

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEA+ +SYSLEVG + RKL W+G PRSIRDSHRKVRDS DGLII RN+AL+FSG
Sbjct: 228 RFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSG 287

Query: 301 GDRKELKLRVTGRIWKEQ 319
            D++ELKLRVTGRIWKE+
Sbjct: 288 SDKEELKLRVTGRIWKEE 305

BLAST of Lag0020126 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 424.9 bits (1091), Expect = 6.1e-119
Identity = 194/218 (88.99%), Postives = 207/218 (94.95%), Query Frame = 0

Query: 115 LALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFL 174
           +ALEKVAESLELPCKYY+LGC  IFPYYSKLKHE+ CNFRPY+CPYAGSEC+AVGDI FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 175 VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAP 234
           VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF CFGQYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 235 VYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNM 294
           VYMAFLRFMGDE++AR Y+YSLEVGG GRK  WEGTPRS+RDSHRKVRDSHDGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 295 ALFFSGGDRKELKLRVTGRIWKE-QNPDAGVCIPNLCS 332
           ALFFSGGD+KELKLRVTGRIWKE QNPD+GVCI ++CS
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQQNPDSGVCITSMCS 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141336.15.1e-20098.79E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia][more]
XP_022984623.12.5e-19998.49E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita maxima][more]
XP_022922771.17.3e-19998.19E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita moschata] >XP_023553000... [more]
XP_022945143.12.1e-19897.58E3 ubiquitin-protein ligase SINAT5-like [Cucurbita moschata] >XP_023541986.1 E3 ... [more]
XP_022968641.16.2e-19897.28E3 ubiquitin-protein ligase SINAT5-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q84JL34.8e-16180.48E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q9STN89.1e-16080.36E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Q8S3N11.2e-15679.22E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Q10L916.5e-13482.95E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q9M2P41.8e-13178.06E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1CIV82.4e-20098.79RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101175... [more]
A0A6J1JB271.2e-19998.49RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111482855 P... [more]
A0A6J1E5133.5e-19998.19RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111430668... [more]
A0A6J1FZZ81.0e-19897.58RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449471... [more]
A0A6J1HVG53.0e-19897.28RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467802 P... [more]
Match NameE-valueIdentityDescription
AT3G61790.13.4e-16280.48Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.16.4e-16180.36Protein with RING/U-box and TRAF-like domains [more]
AT3G58040.11.3e-13278.06seven in absentia of Arabidopsis 2 [more]
AT2G41980.11.5e-13080.62Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.16.1e-11988.99TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 177..311
e-value: 5.9E-38
score: 131.6
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 112..311
e-value: 4.5E-79
score: 265.1
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 32..122
e-value: 3.5E-7
score: 31.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 123..176
e-value: 4.5E-17
score: 64.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..55
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..55
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 1..323
NoneNo IPR availablePANTHERPTHR10315:SF127E3 UBIQUITIN-PROTEIN LIGASEcoord: 1..323
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 68..106
e-value: 1.66097E-14
score: 64.6134
NoneNo IPR availableCDDcd03829Sinacoord: 184..312
e-value: 1.36559E-67
score: 206.084
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 123..315
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 59..110
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 70..106
score: 9.464913
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 123..183
score: 14.091654

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020126.1Lag0020126.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding