Lag0020125 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020125
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr5: 48308721 .. 48311552 (-)
RNA-Seq ExpressionLag0020125
SyntenyLag0020125
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGGACAGTATCGAGTGTGTGTCATCGTCAGATGGTCTCGATGAGGATGAGATCCATCTGCACCACACTCTTCATCCTTATTCCCATTCTCATCATCACCCAGAGTTGTCAGCGTCGAAGCCTCGTAATGGAAACAACAATAGCGCTGTGAACGGCCCCACTGCAACTGCGCCGGCAACTAGCGTCCATGAATTGCTGGAGTGCCCTGTCTGCACTAATTCTATGTATCCTCCCATCCATCAGGTTTGTCTTCGTTGCCCTAATTACTTTCAGAATGGAGATTATTTGGCCCTCCACATTTGGATTTTTGACTTGGGTATGAAAAAGGGGTTTTAAATTTTTTTCCTGCTCGGGGTTTGGATTATCGTTGATCGATCAATTGTTGATCGGAAGTTGTGTGGTATTGTATACTGACAACCCCTATGACATGTAAGTGAATGGATATTACTGAGACATGAAGGCACCACAATGGAATGGACATTACTTTTACAAATAAAAGATAATCATGAAAGTATTTTGATGTTGCTGTTGCTTTGGTCTTGAAAATTGTACGAGCTAGAAACTAAGTTATCTTAGTTTTTGGCATGTTGTATGTTGTATAATGTATTCAATAAACCACCATCAAATTCTCTTTCTTGTTAAGTTTTTCTAGCTCTAAGATTGTATGACAGAGATTAGATTACTACTTGTGCCATTTGTGCTTTTAAGGCGTTTATTTCTACCCTAAGATGCTTGTAGTTTCTATAAATTTGCTTAGGAAATTCTTTTTGGTTTAGTTATAAACATTATTTTGGTAGGCTGATGAAATTTTACTTTTCGCCTTGCTCCCTTTGGGATATCATTTTCCATCATCATAGTTTTTTCTCTTCTCCTTGTATCATTTGAACTGACCTTTATAGGATGTACGTGTCTATATAATCAGGATGAAAGCAGTTGTGAATATGCTTGTTTTATACCAATAATTGTCTGAGGACATATGTTGTTTCTTATAAGACGTGCATTTTATTGGAGATATATTTTCAATGTTAGTGTATAAATGTTGGTTAACTGTGTATGTCCAACTAAAATGTTGCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGCTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCACTGGAGAAGGTGGCAGAGTCACTTGAATTGCCTTGCAAGTATTATTCCTTGGGGTGCCCTGAGATATTTCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTTCAGACCCTATAGTTGCCCCTACGCTGGGTCGGAATGCTCTGCTGTTGGGGATATTCCTTTCCTTGTTGCCCATCTGAGGGACGATCATAAGGTGGACATGCACACTGGCTGCACGTTTAACCATCGCTATGTGAAATCCAATCCAAGGGAAGTGGAGAATGCCACTTGGATGCTTACTGTAAGTCTGATATATCTGACCTCAACTATCAATTGGATTCTCATTTTCTCTTTCTTTGGTAGAACTTAGTTTATCTACGTGAATCGATCCTTACCTGTGATAGATTTGTATGCACACGTCTGTATGTTTATGTAAATATGTATGTATGTATCTGTGGTGTCGCTCGATATCTATTAAGGTATGGGGAGGACAAGGGTTTCAGACCAAACTATGAAATTCTGGGAGATTAGAAGAAGGCTCCAACTAGTTGAAAACAAAAGAAAGATGATGAAAAGTCCGTTATTACATAGCGTGCTGCAAGTTTTATAGGCCATCAAAGTTGGTCCGAAAATTTTGCTGATGATGTGAAAAAGGTATTGCTGCCTGCCTGCTAGTTGATTAGAAATTTGGTAATGGATCACGATCATTTTATTTTATTTTATGGAACTTTGACCTTTACTGTGTTTGATGATTGCATTATCAAAGGGTTAAGAGTACATGTTTTTATATAATTTTTTTCAGTTTCCTTTTACCCCAAGTGATGGATTTATTTAGGGGCTTTGGGGTAAGAGAAACAATAGGATTTTTAGAGGGTGCGAGAGGGATTCAGCTAAGGTTTGGTCCCTTGTGAGATATTATGTTTCTCTTTGGACGTGGGTGATGCGTTTATTCTGTAACTATTCCATATGTCTTATCATTTTGGATTGGAGCCCCTTTATCTAATGGGGTCTCCCTCTTTTTTTGTGGGCGGGTTGTTTTTTGATCGTCCTTGTATTCTTTCATTTTTTTCTCAATGAAAGCATGGTTATTCAAAAAAAAAAAAAAAAAAATCTGTACAAACGAAAGCTAATATGGTCACGGGATGCCAAAACCACTCATAAATGCCATATATGGCTTGTTATGTGTTTGATTAAACAAGGCCTTCGCGCCAGAAGGAGACTCATTCTGCTGGACATTCCTCATGGTTTACTCCATATTTTAGATTGATCCCGTTTCTTGTGGCTTGCTCTTCTCACATTTTGCAGGTTTTTAATTGTTTCGGTCAATACTTCTGCCTTCACTTTGAAGCCTTTCAGCTTGGCATGGCCCCAGTCTACATGGCTTTCCTTCGATTCATGGGCGATGAGAACGAGGCTCGAACCTACAGCTACAGTCTTGAGGTTGGTGGATATGGGCGGAAACTGATATGGGAGGGTACACCACGAAGTATTAGAGATAGTCATCGAAAAGTCAGGGACAGCCACGATGGATTGATCATTCAGAGAAATATGGCTCTTTTCTTCTCTGGTGGGGATAGAAAGGAACTGAAGCTGAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCAGATGCTGGGGTCTGCATACCGAACCTTTGTAGCTAA

mRNA sequence

ATGGATTTGGACAGTATCGAGTGTGTGTCATCGTCAGATGGTCTCGATGAGGATGAGATCCATCTGCACCACACTCTTCATCCTTATTCCCATTCTCATCATCACCCAGAGTTGTCAGCGTCGAAGCCTCGTAATGGAAACAACAATAGCGCTGTGAACGGCCCCACTGCAACTGCGCCGGCAACTAGCGTCCATGAATTGCTGGAGTGCCCTGTCTGCACTAATTCTATGTATCCTCCCATCCATCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGCTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCACTGGAGAAGGTGGCAGAGTCACTTGAATTGCCTTGCAAGTATTATTCCTTGGGGTGCCCTGAGATATTTCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTTCAGACCCTATAGTTGCCCCTACGCTGGGTCGGAATGCTCTGCTGTTGGGGATATTCCTTTCCTTGTTGCCCATCTGAGGGACGATCATAAGGTGGACATGCACACTGGCTGCACGTTTAACCATCGCTATGTGAAATCCAATCCAAGGGAAGTGGAGAATGCCACTTGGATGCTTACTGTTTTTAATTGTTTCGGTCAATACTTCTGCCTTCACTTTGAAGCCTTTCAGCTTGGCATGGCCCCAGTCTACATGGCTTTCCTTCGATTCATGGGCGATGAGAACGAGGCTCGAACCTACAGCTACAGTCTTGAGGTTGGTGGATATGGGCGGAAACTGATATGGGAGGGTACACCACGAAGTATTAGAGATAGTCATCGAAAAGTCAGGGACAGCCACGATGGATTGATCATTCAGAGAAATATGGCTCTTTTCTTCTCTGGTGGGGATAGAAAGGAACTGAAGCTGAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCAGATGCTGGGGTCTGCATACCGAACCTTTGTAGCTAA

Coding sequence (CDS)

ATGGATTTGGACAGTATCGAGTGTGTGTCATCGTCAGATGGTCTCGATGAGGATGAGATCCATCTGCACCACACTCTTCATCCTTATTCCCATTCTCATCATCACCCAGAGTTGTCAGCGTCGAAGCCTCGTAATGGAAACAACAATAGCGCTGTGAACGGCCCCACTGCAACTGCGCCGGCAACTAGCGTCCATGAATTGCTGGAGTGCCCTGTCTGCACTAATTCTATGTATCCTCCCATCCATCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGCTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCACTGGAGAAGGTGGCAGAGTCACTTGAATTGCCTTGCAAGTATTATTCCTTGGGGTGCCCTGAGATATTTCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTTCAGACCCTATAGTTGCCCCTACGCTGGGTCGGAATGCTCTGCTGTTGGGGATATTCCTTTCCTTGTTGCCCATCTGAGGGACGATCATAAGGTGGACATGCACACTGGCTGCACGTTTAACCATCGCTATGTGAAATCCAATCCAAGGGAAGTGGAGAATGCCACTTGGATGCTTACTGTTTTTAATTGTTTCGGTCAATACTTCTGCCTTCACTTTGAAGCCTTTCAGCTTGGCATGGCCCCAGTCTACATGGCTTTCCTTCGATTCATGGGCGATGAGAACGAGGCTCGAACCTACAGCTACAGTCTTGAGGTTGGTGGATATGGGCGGAAACTGATATGGGAGGGTACACCACGAAGTATTAGAGATAGTCATCGAAAAGTCAGGGACAGCCACGATGGATTGATCATTCAGAGAAATATGGCTCTTTTCTTCTCTGGTGGGGATAGAAAGGAACTGAAGCTGAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCAGATGCTGGGGTCTGCATACCGAACCTTTGTAGCTAA

Protein sequence

MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Homology
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BLAST of Lag0020125 vs. NCBI nr
Match: XP_022141336.1 (E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia])

HSP 1 Score: 704.5 bits (1817), Expect = 4.3e-199
Identity = 326/331 (98.49%), Postives = 330/331 (99.70%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

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BLAST of Lag0020125 vs. NCBI nr
Match: XP_022984623.1 (E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 704.1 bits (1816), Expect = 5.6e-199
Identity = 325/331 (98.19%), Postives = 330/331 (99.70%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS++KPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

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BLAST of Lag0020125 vs. NCBI nr
Match: XP_022922771.1 (E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita moschata] >XP_023553000.1 E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita pepo subsp. pepo] >KAG7014703.1 E3 ubiquitin-protein ligase SINAT4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 702.6 bits (1812), Expect = 1.6e-198
Identity = 324/331 (97.89%), Postives = 330/331 (99.70%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS++KPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

[Close]
BLAST of Lag0020125 vs. NCBI nr
Match: XP_023541988.1 (E3 ubiquitin-protein ligase SINAT5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699.5 bits (1804), Expect = 1.4e-197
Identity = 324/331 (97.89%), Postives = 328/331 (99.09%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDED+IHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVN PTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDDIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNDPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           +TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  STSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNP+EVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPKEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQN DAG CIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNADAGACIPNLCS 331

[Close]
BLAST of Lag0020125 vs. NCBI nr
Match: XP_022945143.1 (E3 ubiquitin-protein ligase SINAT5-like [Cucurbita moschata] >XP_023541986.1 E3 ubiquitin-protein ligase SINAT5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 698.4 bits (1801), Expect = 3.1e-197
Identity = 323/331 (97.58%), Postives = 329/331 (99.40%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLH YSHSHHHPELSA+KPRNGNNNSAV GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

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BLAST of Lag0020125 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 2.8e-161
Identity = 270/332 (81.33%), Postives = 286/332 (86.14%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDS++C S+ D  D++EIH     +     HHH   + +     N N+A +G   T  
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIHQDRHSYASVSKHHHTNNNTT-----NVNAAASGLLPT-- 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
            TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCK+ SLGCPEIFPYYSKLKHE +CNFRPYSCPYAGSECS  GDIPFLVAHLRD
Sbjct: 121 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE EAR Y+YSLEVGGYGRKLIWEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPD---AGVCIPNL 330
           GDRKELKLRVTGRIWKEQ       G CIPNL
Sbjct: 301 GDRKELKLRVTGRIWKEQQQSGEGGGACIPNL 325

[Close]
BLAST of Lag0020125 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 1.7e-158
Identity = 269/336 (80.06%), Postives = 291/336 (86.61%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAV----NGPT 60
           M+ DS+ECVSS+           + +H   + H   + S++K   G   +AV     GPT
Sbjct: 1   METDSMECVSSTG----------NEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPT 60

Query: 61  ATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLA 120
           ATAPATSV+ELLECPVCT SMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLA
Sbjct: 61  ATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLA 120

Query: 121 LEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVA 180
           LEKVAESLELPCK+Y+LGCPEIFPYYSKLKHE+LCNFRPYSCPYAGSEC  VGDIPFLVA
Sbjct: 121 LEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVA 180

Query: 181 HLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVY 240
           HLRDDHKVDMH G TFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGM PVY
Sbjct: 181 HLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVY 240

Query: 241 MAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMAL 300
           MAFLRFMGDE +AR+YSYSLEVGG GRKL WEGTPRSIRDSHRKVRDS+DGLIIQRNMAL
Sbjct: 241 MAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMAL 300

Query: 301 FFSGGDRKELKLRVTGRIWKEQ-NPDAGVCIPNLCS 332
           FFSGGDRKELKLRVTG+IWKEQ +PD+G+ IPNL S
Sbjct: 301 FFSGGDRKELKLRVTGKIWKEQHSPDSGLSIPNLSS 326

[Close]
BLAST of Lag0020125 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 552.7 bits (1423), Expect = 2.7e-156
Identity = 263/332 (79.22%), Postives = 285/332 (85.84%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           M+ DSI+ V     +D+DEIH  H            + S++K + G            +P
Sbjct: 1   METDSIDSV-----IDDDEIHQKH------------QFSSTKSQGG-------ATVVISP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSV+ELLECPVCTNSMYPPIHQCHNGHTLCSTCK+RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYY+LGC  IFPYYSKLKHE+ CNFRPYSCPYAGSEC+AVGDI FLVAHLRD
Sbjct: 121 AESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE++AR Y+YSLEVGG GRK  WEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKE-QNPDAGVCIPNLCS 332
           GD+KELKLRVTGRIWKE QNPD+GVCI ++CS
Sbjct: 301 GDKKELKLRVTGRIWKEQQNPDSGVCITSMCS 308

[Close]
BLAST of Lag0020125 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 2.2e-134
Identity = 215/258 (83.33%), Postives = 235/258 (91.09%), Query Frame = 0 

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ++SV ELLECPVC ++MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKV
Sbjct: 44  SSSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 103

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           A SLELPCKY + GC  I+PYY KLKHE+ C +RPYSCPYAGSEC+  GDIP+LV HL+D
Sbjct: 104 AASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKD 163

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH GCTFNHRYVKSNP EVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVY+AFL
Sbjct: 164 DHKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFL 223

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGD+ EA+ YSYSLEVGG GRK+IW+G PRSIRDSHRKVRDS+DGLIIQRNMALFFSG
Sbjct: 224 RFMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSG 283

Query: 301 GDRKELKLRVTGRIWKEQ 319
           G+RKELKLRVTGRIWKEQ
Sbjct: 284 GERKELKLRVTGRIWKEQ 301

[Close]
BLAST of Lag0020125 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 8.0e-132
Identity = 220/287 (76.66%), Postives = 239/287 (83.28%), Query Frame = 0 

Query: 37  ELSASKPRNGNN-----NSAVNGPTATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLC 96
           E+  +K    NN      SA  G         V+ELLECPVCTN MYPPIHQC NGHTLC
Sbjct: 22  EVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLC 81

Query: 97  STCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALC 156
           S CK RV N CPTCR ELG+IRCLALEKVAESLE+PC+Y +LGC +IFPYYSKLKHE  C
Sbjct: 82  SNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHC 141

Query: 157 NFRPYSCPYAGSECSAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWML 216
            FRPY+CPYAGSECS  GDIP LV HL+DDHKVDMH GCTFNHRYVKSNP EVENATWML
Sbjct: 142 RFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWML 201

Query: 217 TVFNCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTP 276
           TVFNCFG+ FCLHFEAFQLGMAPVYMAFLRFMGDENEA+ +SYSLEVG +GRKL W+G P
Sbjct: 202 TVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIP 261

Query: 277 RSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 319
           RSIRDSHRKVRDS DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 262 RSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

[Close]
BLAST of Lag0020125 vs. ExPASy TrEMBL
Match: A0A6J1CIV8 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111011754 PE=3 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.1e-199
Identity = 326/331 (98.49%), Postives = 330/331 (99.70%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

[Close]
BLAST of Lag0020125 vs. ExPASy TrEMBL
Match: A0A6J1JB27 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111482855 PE=3 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 2.7e-199
Identity = 325/331 (98.19%), Postives = 330/331 (99.70%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS++KPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

[Close]
BLAST of Lag0020125 vs. ExPASy TrEMBL
Match: A0A6J1E513 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111430668 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 7.9e-199
Identity = 324/331 (97.89%), Postives = 330/331 (99.70%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS++KPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

[Close]
BLAST of Lag0020125 vs. ExPASy TrEMBL
Match: A0A6J1FZZ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449471 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.5e-197
Identity = 323/331 (97.58%), Postives = 329/331 (99.40%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLH YSHSHHHPELSA+KPRNGNNNSAV GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

[Close]
BLAST of Lag0020125 vs. ExPASy TrEMBL
Match: A0A6J1HVG5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467802 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 4.3e-197
Identity = 322/331 (97.28%), Postives = 328/331 (99.09%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDG DEDEIHLHHTLH YSHSHHHPELSA+KPRNGNNNSAV GPTATAP
Sbjct: 1   MDLDSIECVSSSDGFDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

[Close]
BLAST of Lag0020125 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 569.3 bits (1466), Expect = 2.0e-162
Identity = 270/332 (81.33%), Postives = 286/332 (86.14%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDS++C S+ D  D++EIH     +     HHH   + +     N N+A +G   T  
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIHQDRHSYASVSKHHHTNNNTT-----NVNAAASGLLPT-- 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
            TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCK+ SLGCPEIFPYYSKLKHE +CNFRPYSCPYAGSECS  GDIPFLVAHLRD
Sbjct: 121 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE EAR Y+YSLEVGGYGRKLIWEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPD---AGVCIPNL 330
           GDRKELKLRVTGRIWKEQ       G CIPNL
Sbjct: 301 GDRKELKLRVTGRIWKEQQQSGEGGGACIPNL 325

[Close]
BLAST of Lag0020125 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 560.1 bits (1442), Expect = 1.2e-159
Identity = 269/336 (80.06%), Postives = 291/336 (86.61%), Query Frame = 0 

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAV----NGPT 60
           M+ DS+ECVSS+           + +H   + H   + S++K   G   +AV     GPT
Sbjct: 1   METDSMECVSSTG----------NEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPT 60

Query: 61  ATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLA 120
           ATAPATSV+ELLECPVCT SMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLA
Sbjct: 61  ATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLA 120

Query: 121 LEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVA 180
           LEKVAESLELPCK+Y+LGCPEIFPYYSKLKHE+LCNFRPYSCPYAGSEC  VGDIPFLVA
Sbjct: 121 LEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVA 180

Query: 181 HLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVY 240
           HLRDDHKVDMH G TFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGM PVY
Sbjct: 181 HLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVY 240

Query: 241 MAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMAL 300
           MAFLRFMGDE +AR+YSYSLEVGG GRKL WEGTPRSIRDSHRKVRDS+DGLIIQRNMAL
Sbjct: 241 MAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMAL 300

Query: 301 FFSGGDRKELKLRVTGRIWKEQ-NPDAGVCIPNLCS 332
           FFSGGDRKELKLRVTG+IWKEQ +PD+G+ IPNL S
Sbjct: 301 FFSGGDRKELKLRVTGKIWKEQHSPDSGLSIPNLSS 326

[Close]
BLAST of Lag0020125 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 471.5 bits (1212), Expect = 5.7e-133
Identity = 220/287 (76.66%), Postives = 239/287 (83.28%), Query Frame = 0 

Query: 37  ELSASKPRNGNN-----NSAVNGPTATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLC 96
           E+  +K    NN      SA  G         V+ELLECPVCTN MYPPIHQC NGHTLC
Sbjct: 22  EVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLC 81

Query: 97  STCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALC 156
           S CK RV N CPTCR ELG+IRCLALEKVAESLE+PC+Y +LGC +IFPYYSKLKHE  C
Sbjct: 82  SNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHC 141

Query: 157 NFRPYSCPYAGSECSAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWML 216
            FRPY+CPYAGSECS  GDIP LV HL+DDHKVDMH GCTFNHRYVKSNP EVENATWML
Sbjct: 142 RFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWML 201

Query: 217 TVFNCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTP 276
           TVFNCFG+ FCLHFEAFQLGMAPVYMAFLRFMGDENEA+ +SYSLEVG +GRKL W+G P
Sbjct: 202 TVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIP 261

Query: 277 RSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 319
           RSIRDSHRKVRDS DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 262 RSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

[Close]
BLAST of Lag0020125 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 466.1 bits (1198), Expect = 2.4e-131
Identity = 210/258 (81.40%), Postives = 229/258 (88.76%), Query Frame = 0 

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           +  V+ELLECPVCTN MYPPIHQC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKV
Sbjct: 48  SNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKV 107

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLE+PC+Y +LGC +IFPYYSKLKHE  C FR YSCPYAGSECS  GDIP LV HL+D
Sbjct: 108 AESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKD 167

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH GCTFNHRYVKSNP EVENATWMLTVFNCFG+ FCLHFEAFQLGMAPVYMAFL
Sbjct: 168 DHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFL 227

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEA+ +SYSLEVG + RKL W+G PRSIRDSHRKVRDS DGLII RN+AL+FSG
Sbjct: 228 RFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSG 287

Query: 301 GDRKELKLRVTGRIWKEQ 319
            D++ELKLRVTGRIWKE+
Sbjct: 288 SDKEELKLRVTGRIWKEE 305

[Close]
BLAST of Lag0020125 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 426.0 bits (1094), Expect = 2.7e-119
Identity = 195/218 (89.45%), Postives = 207/218 (94.95%), Query Frame = 0 

Query: 115 LALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFL 174
           +ALEKVAESLELPCKYY+LGC  IFPYYSKLKHE+ CNFRPYSCPYAGSEC+AVGDI FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 175 VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAP 234
           VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF CFGQYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 235 VYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNM 294
           VYMAFLRFMGDE++AR Y+YSLEVGG GRK  WEGTPRS+RDSHRKVRDSHDGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 295 ALFFSGGDRKELKLRVTGRIWKE-QNPDAGVCIPNLCS 332
           ALFFSGGD+KELKLRVTGRIWKE QNPD+GVCI ++CS
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQQNPDSGVCITSMCS 232

The following BLAST results are available for this feature:
BLAST of Lag0020125 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022141336.14.3e-19998.49E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia][more]
XP_022984623.15.6e-19998.19E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita maxima][more]
XP_022922771.11.6e-19897.89E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita moschata] >XP_023553000... [more]
XP_023541988.11.4e-19797.89E3 ubiquitin-protein ligase SINAT5-like [Cucurbita pepo subsp. pepo][more]
XP_022945143.13.1e-19797.58E3 ubiquitin-protein ligase SINAT5-like [Cucurbita moschata] >XP_023541986.1 E3 ... [more]
BLAST of Lag0020125 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q84JL32.8e-16181.33E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q9STN81.7e-15880.06E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Q8S3N12.7e-15679.22E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Q10L912.2e-13483.33E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q9M2P48.0e-13276.66E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
BLAST of Lag0020125 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CIV82.1e-19998.49RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101175... [more]
A0A6J1JB272.7e-19998.19RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111482855 P... [more]
A0A6J1E5137.9e-19997.89RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111430668... [more]
A0A6J1FZZ81.5e-19797.58RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449471... [more]
A0A6J1HVG54.3e-19797.28RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467802 P... [more]
BLAST of Lag0020125 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G61790.12.0e-16281.33Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.11.2e-15980.06Protein with RING/U-box and TRAF-like domains [more]
AT3G58040.15.7e-13376.66seven in absentia of Arabidopsis 2 [more]
AT2G41980.12.4e-13181.40Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.12.7e-11989.45TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 177..311
e-value: 4.0E-38
score: 132.2
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 112..311
e-value: 5.4E-79
score: 264.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 29..122
e-value: 3.4E-7
score: 31.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 123..176
e-value: 3.5E-17
score: 64.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..58
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..58
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 1..323
NoneNo IPR availablePANTHERPTHR10315:SF127E3 UBIQUITIN-PROTEIN LIGASEcoord: 1..323
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 68..106
e-value: 1.42945E-14
score: 64.9986
NoneNo IPR availableCDDcd03829Sinacoord: 184..312
e-value: 2.40225E-67
score: 205.313
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 59..110
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 123..315
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 70..106
score: 9.464913
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 123..183
score: 14.108719

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020125.1Lag0020125.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding