Lag0020086 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020086
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionethylene-responsive transcription factor CRF4-like
Locationchr5: 47960346 .. 47961338 (+)
RNA-Seq ExpressionLag0020086
SyntenyLag0020086
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGATTCTCTGTTTTTTCCGGTTAAGTTCACAGAGCACCGCAACTTCACCAACAAATTCTCCTCCAAGAAACACCACTCGCCGGAGAATCGACTCGTACGGATTTCTGTGACCGACCCAGATGCCACGGATTCGTCCAGCGATGAAGAAGGGGAGTTTTTCGAGAGACAGAGAGTCAAGAAATACATCAATGAGATCAAAATCCAAACTGGGTGTCGGAATGGACTTGTACCCAGTTGCCGGAAGAGGCCTGCCGGTGAGGGAGAGGGTTCCCGGCGGCCGGTCAAGGCGGCTCCGGCCACCAATGGGAAGAAATTCCGTGGCGTACGGCAGCGTCCGTGGGGGAAGTGGGCGGCAGAGATTAGAGATCCGGCTCGCCGTGTGCGGCTGTGGTTGGGGACTTACGACACGGCGGAGGAAGCTGCCATGGTGTACGACAACGCCGCCATTCAGCTCCGAGGGCCGGACGCTTTGACGAACTTTGCTACCCCGCCGCCGCCGCTGCCGGAGAAGGAAGTGGAGGCGGCTAATATCCCCTCTGTTTCCGGCTCGTATTACGACTCTGGCGAGGAGTCGCACAATTTGTCATCGCCGACTTCGGTTCTCCGCTTCAGAACGCCGTCGCCGGAGGAACCCGAGAAGCCACAAAAATCCGACGAGTTTCAGAAACCGCCGCGGGCGCCGCTTCCGCCGTTCGTCGACGACCAATTTCACGAATGTCAAGGGGAAACTTCTTTTTCCGAGGAGTATTACACCGACTTCCCCAAATTCGACGATGTGTTCAAGGTTCCGAGCCCCGATTCGCCGATTTTGTTCGACGAACAGCCGCTGTTCTTAGAAGAAAGTATATGGAACGAAGGTTTCAGTGAAATGTTTACGAATTTGCCCGAAGATTTTGGGTCGCCATTGTTGTCTTCTTCGATAGGTCAAGGGGGCGACGACTATTTCCAAGATATTTTGATGGGGTCGGACCCTTTGGTGGTTCTGTGA

mRNA sequence

ATGGATGATTCTCTGTTTTTTCCGGTTAAGTTCACAGAGCACCGCAACTTCACCAACAAATTCTCCTCCAAGAAACACCACTCGCCGGAGAATCGACTCGTACGGATTTCTGTGACCGACCCAGATGCCACGGATTCGTCCAGCGATGAAGAAGGGGAGTTTTTCGAGAGACAGAGAGTCAAGAAATACATCAATGAGATCAAAATCCAAACTGGGTGTCGGAATGGACTTGTACCCAGTTGCCGGAAGAGGCCTGCCGGTGAGGGAGAGGGTTCCCGGCGGCCGGTCAAGGCGGCTCCGGCCACCAATGGGAAGAAATTCCGTGGCGTACGGCAGCGTCCGTGGGGGAAGTGGGCGGCAGAGATTAGAGATCCGGCTCGCCGTGTGCGGCTGTGGTTGGGGACTTACGACACGGCGGAGGAAGCTGCCATGGTGTACGACAACGCCGCCATTCAGCTCCGAGGGCCGGACGCTTTGACGAACTTTGCTACCCCGCCGCCGCCGCTGCCGGAGAAGGAAGTGGAGGCGGCTAATATCCCCTCTGTTTCCGGCTCGTATTACGACTCTGGCGAGGAGTCGCACAATTTGTCATCGCCGACTTCGGTTCTCCGCTTCAGAACGCCGTCGCCGGAGGAACCCGAGAAGCCACAAAAATCCGACGAGTTTCAGAAACCGCCGCGGGCGCCGCTTCCGCCGTTCGTCGACGACCAATTTCACGAATGTCAAGGGGAAACTTCTTTTTCCGAGGAGTATTACACCGACTTCCCCAAATTCGACGATGTGTTCAAGGTTCCGAGCCCCGATTCGCCGATTTTGTTCGACGAACAGCCGCTGTTCTTAGAAGAAAGTATATGGAACGAAGGTTTCAGTGAAATGTTTACGAATTTGCCCGAAGATTTTGGGTCGCCATTGTTGTCTTCTTCGATAGGTCAAGGGGGCGACGACTATTTCCAAGATATTTTGATGGGGTCGGACCCTTTGGTGGTTCTGTGA

Coding sequence (CDS)

ATGGATGATTCTCTGTTTTTTCCGGTTAAGTTCACAGAGCACCGCAACTTCACCAACAAATTCTCCTCCAAGAAACACCACTCGCCGGAGAATCGACTCGTACGGATTTCTGTGACCGACCCAGATGCCACGGATTCGTCCAGCGATGAAGAAGGGGAGTTTTTCGAGAGACAGAGAGTCAAGAAATACATCAATGAGATCAAAATCCAAACTGGGTGTCGGAATGGACTTGTACCCAGTTGCCGGAAGAGGCCTGCCGGTGAGGGAGAGGGTTCCCGGCGGCCGGTCAAGGCGGCTCCGGCCACCAATGGGAAGAAATTCCGTGGCGTACGGCAGCGTCCGTGGGGGAAGTGGGCGGCAGAGATTAGAGATCCGGCTCGCCGTGTGCGGCTGTGGTTGGGGACTTACGACACGGCGGAGGAAGCTGCCATGGTGTACGACAACGCCGCCATTCAGCTCCGAGGGCCGGACGCTTTGACGAACTTTGCTACCCCGCCGCCGCCGCTGCCGGAGAAGGAAGTGGAGGCGGCTAATATCCCCTCTGTTTCCGGCTCGTATTACGACTCTGGCGAGGAGTCGCACAATTTGTCATCGCCGACTTCGGTTCTCCGCTTCAGAACGCCGTCGCCGGAGGAACCCGAGAAGCCACAAAAATCCGACGAGTTTCAGAAACCGCCGCGGGCGCCGCTTCCGCCGTTCGTCGACGACCAATTTCACGAATGTCAAGGGGAAACTTCTTTTTCCGAGGAGTATTACACCGACTTCCCCAAATTCGACGATGTGTTCAAGGTTCCGAGCCCCGATTCGCCGATTTTGTTCGACGAACAGCCGCTGTTCTTAGAAGAAAGTATATGGAACGAAGGTTTCAGTGAAATGTTTACGAATTTGCCCGAAGATTTTGGGTCGCCATTGTTGTCTTCTTCGATAGGTCAAGGGGGCGACGACTATTTCCAAGATATTTTGATGGGGTCGGACCCTTTGGTGGTTCTGTGA

Protein sequence

MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRVKKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPLPEKEVEAANIPSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFHECQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPEDFGSPLLSSSIGQGGDDYFQDILMGSDPLVVL
Homology
BLAST of Lag0020086 vs. NCBI nr
Match: XP_004149454.1 (ethylene-responsive transcription factor CRF4 [Cucumis sativus] >KGN57872.1 hypothetical protein Csa_011137 [Cucumis sativus])

HSP 1 Score: 553.1 bits (1424), Expect = 1.6e-153
Identity = 276/331 (83.38%), Postives = 298/331 (90.03%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           M+D+LF PVKFTEHRNFTNKFS+KKHH  E+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAA 120
           KKYINEIKIQ+GCRN L+PSCRKRPAG+    RR  K  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PLPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP P+PEKEVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFH 240
           PSVSGSYYDS EESHNLSSPTSVL FRTPSPEE EKP KSD+ QKPP     PFVDDQFH
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPP----APFVDDQFH 240

Query: 241 ECQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPED 300
           ECQGETSF+EEY+T+FP+FD  FK PSP++PI  D+QPLF E+SIWN+ FSE+FTNLPED
Sbjct: 241 ECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPED 300

Query: 301 FGSPLLSSSIGQGGDDYFQDILMGSDPLVVL 331
           FGSPLLSSSIGQGGDDYFQDILMGSDPLVVL
Sbjct: 301 FGSPLLSSSIGQGGDDYFQDILMGSDPLVVL 326

BLAST of Lag0020086 vs. NCBI nr
Match: KAG7013289.1 (Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 547.4 bits (1409), Expect = 8.7e-152
Identity = 272/330 (82.42%), Postives = 295/330 (89.39%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           M+D+LF PVKFTEHR+FTNKFSS+KH   +NR+VRI+VTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRSFTNKFSSRKHPPSQNRVVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAA 120
           K+Y+NEIKIQT  RN L+PS RKRPA EG  SRRPVKAAP  NGKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEIKIQTASRNTLLPSARKRPAAEGSDSRRPVKAAPVMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPLPEKEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA PPPPLPE EVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFHE 240
           SVSGSY DS EESHNLSSPTSVLRFRTPS EE EKPQ S  FQKPP  PLPP  DDQ  E
Sbjct: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSSVFQKPPPPPLPPMADDQLRE 240

Query: 241 CQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPEDF 300
           CQGETSF EEYYTDFPKF+DVF++P  +SP+  D+QPL+LEESI+NEGFS++FTNLP+DF
Sbjct: 241 CQGETSFYEEYYTDFPKFEDVFQLPFSESPMFLDDQPLYLEESIYNEGFSDLFTNLPDDF 300

Query: 301 GSPLLSSSIGQGGDDYFQDILMGSDPLVVL 331
           GSPLLSSSI Q G+D+FQDILMGSDPLVVL
Sbjct: 301 GSPLLSSSISQVGEDFFQDILMGSDPLVVL 330

BLAST of Lag0020086 vs. NCBI nr
Match: XP_008439327.1 (PREDICTED: ethylene-responsive transcription factor CRF4-like [Cucumis melo] >KAA0033741.1 ethylene-responsive transcription factor CRF4-like [Cucumis melo var. makuwa] >TYJ99880.1 ethylene-responsive transcription factor CRF4-like [Cucumis melo var. makuwa])

HSP 1 Score: 543.9 bits (1400), Expect = 9.6e-151
Identity = 275/332 (82.83%), Postives = 295/332 (88.86%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           M+D+LF PVKFTEHRNFTNKFS+KKHH  E+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAA 120
           KKYINEIKIQ+GCRN L+PSCRKRPAG+    RR  K  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PLPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP  P+PEKEVE  NI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFH 240
           PSVSGSYYDS EESHNLSSPTSVL FRTPSPEE EKP KSD+ QKPP     PFVDDQFH
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPP----APFVDDQFH 240

Query: 241 ECQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPED 300
           ECQGETSFSEEY+T+FP+FD  FK PSP+SPI  D+QPLF E+SIWN+ FSE+FTNLPED
Sbjct: 241 ECQGETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNLPED 300

Query: 301 FGSPLL-SSSIGQGGDDYFQDILMGSDPLVVL 331
           FGSPLL SSSIGQGGDDYFQDIL GSDPLVVL
Sbjct: 301 FGSPLLSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of Lag0020086 vs. NCBI nr
Match: XP_038900136.1 (ethylene-responsive transcription factor CRF4-like [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.6e-150
Identity = 274/331 (82.78%), Postives = 294/331 (88.82%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           M+D+LF PVKFTEHRNFTNKFS+KKHH  E+R+VRISVTDPDATDSSSDE+ ++FERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHHSESRVVRISVTDPDATDSSSDED-DYFERQRV 60

Query: 61  KKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAA 120
           KKYINEIKIQ+GCRN L+PSCRKRPAGE    +R  K AP  NGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGERSDFQRQGKVAPPMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PLPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP  +PEKEVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVSMPEKEVEMANI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFH 240
           PSVSGSYYDS EESHNLSSPTSVL FRTPSPEE EKP KSDE Q PP     PF DDQFH
Sbjct: 181 PSVSGSYYDSCEESHNLSSPTSVLHFRTPSPEESEKPPKSDEVQNPP----APFTDDQFH 240

Query: 241 ECQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPED 300
           ECQGETSFSEE++T+FP+FD  FK PSP+SPI  D+QPLF E+SIWNE FSE+FTNLPED
Sbjct: 241 ECQGETSFSEEFHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNEDFSEIFTNLPED 300

Query: 301 FGSPLLSSSIGQGGDDYFQDILMGSDPLVVL 331
           FGSPLLSSSI QGGDDYFQDILMGSDPLVVL
Sbjct: 301 FGSPLLSSSISQGGDDYFQDILMGSDPLVVL 326

BLAST of Lag0020086 vs. NCBI nr
Match: XP_022945589.1 (ethylene-responsive transcription factor CRF4-like [Cucurbita moschata])

HSP 1 Score: 540.0 bits (1390), Expect = 1.4e-149
Identity = 270/330 (81.82%), Postives = 292/330 (88.48%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MDD+LF PVKFTEHR+FTNKFSS+K    +NR+VRI+VTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MDDTLFSPVKFTEHRSFTNKFSSRKQPPSQNRVVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAA 120
           K+Y+NEIKIQT  RN L+PS RKRPA EG  SRRPVKAAP  NGKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEIKIQTAPRNTLLPSARKRPAAEGSDSRRPVKAAPVMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPLPEKEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA PPPPLPE EVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFHE 240
           SVSGSY DS EESHNLSSP+SVLRFRTPS EE EKPQ S  FQKPP  PLPP  D Q  E
Sbjct: 181 SVSGSYDDSCEESHNLSSPSSVLRFRTPSLEESEKPQNSSVFQKPPPPPLPPMADHQLRE 240

Query: 241 CQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPEDF 300
           CQGETSF EEYYTDFPKF+DVF++P  +SP+  D+QPL+LEESI+N+GFS+ FTNLP+DF
Sbjct: 241 CQGETSFYEEYYTDFPKFEDVFQLPFSESPMFLDDQPLYLEESIYNDGFSDWFTNLPDDF 300

Query: 301 GSPLLSSSIGQGGDDYFQDILMGSDPLVVL 331
           GSPLLSSSI Q G+DYFQDILMGSDPLVVL
Sbjct: 301 GSPLLSSSISQVGEDYFQDILMGSDPLVVL 330

BLAST of Lag0020086 vs. ExPASy Swiss-Prot
Match: Q9SUE3 (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 200.7 bits (509), Expect = 2.6e-50
Identity = 142/336 (42.26%), Postives = 196/336 (58.33%), Query Frame = 0

Query: 8   PVKFTEH----RNFTNKFSSKKHHSPEN--RLVRISVTDPDATDSSSDEEGEFFERQRVK 67
           PVK+TEH    R +T K S ++  S  +  RLVR+S+TD DATDSSSDEE   F R+RVK
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVK 70

Query: 68  KYINEIKIQ-----TGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWG 127
           + INEI+++     TG  +      RKR   +    +  V      N KK+RGVRQRPWG
Sbjct: 71  RLINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKPSVS---GQNQKKYRGVRQRPWG 130

Query: 128 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPLPEKEVEA 187
           KWAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNF   P P P +E E 
Sbjct: 131 KWAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQ 190

Query: 188 ANIPSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEP----EKPQKSDEFQKPPRAPLPP 247
               ++S S  +S ++S +LSSPTSVL ++T   EEP     KP K  EF +P + P+  
Sbjct: 191 EPESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVK-QEFLEPEQEPI-- 250

Query: 248 FVDDQFHECQGETSFSEEYY-TDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSE 307
                +H  +G T+ +++ +  D    D+ F    PD  I   +QP+   +   N+ F++
Sbjct: 251 ----SWHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFND 310

Query: 308 MF---TNLPEDFGSPL--LSSSIGQGGDDYFQDILM 323
           +    +N  E + S +  + SS     D    D+L+
Sbjct: 311 LMLFDSNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

BLAST of Lag0020086 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.9e-49
Identity = 158/353 (44.76%), Postives = 201/353 (56.94%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGE-----FFERQRVKKY 68
           +KFTEH+  T    S+  ++ + R++RISVTDPDATDSSSD+E E       +R+RVKK+
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  INEI-----KIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAA--PATNGKKFRGVRQRPWG 128
           +NE+      + TG    +    R++ A + E +  PV +A    T  KKFRGVRQRPWG
Sbjct: 72  VNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWG 131

Query: 129 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA-TP--------PP 188
           KWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+ TP        PP
Sbjct: 132 KWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPP 191

Query: 189 PLPEKEVEAAN---IPSVSGSYYDSGEESHN-LSSPTSVLRFRTPSPEEPEKPQKSDEFQ 248
           P P K+ +  N     SV+ S   S   S++ L SP SVLR           P   DEF 
Sbjct: 192 PSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLR----------SPFAVDEFS 251

Query: 249 KPPRAPLPPFVDDQFHECQGETSFSEEYYTDF--PKF--DDVFKVPSPDSPILFDEQPLF 308
               +P+   V     E    T+ SE  ++DF  P F  DDVF   S   P       LF
Sbjct: 252 GISSSPVAAVV---VKEEPSMTTVSET-FSDFSAPLFSDDDVFDFRSSVVPDYLGGD-LF 311

Query: 309 LEESIWNEGFSEMFTNLPEDFGSPLLSSSIGQGGDDYFQDI--LMGSDPLVVL 331
            E+    +  ++M  N   DFGS L S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 GEDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of Lag0020086 vs. ExPASy Swiss-Prot
Match: Q9FK12 (Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN=CRF3 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.3e-46
Identity = 146/363 (40.22%), Postives = 199/363 (54.82%), Query Frame = 0

Query: 1   MDDSL-FFPVKFTEHR----NFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGE-- 60
           MD+ + F P+K+TEH+     +T K S K       + V I  TDPDATDSSSDE+ E  
Sbjct: 1   MDEYIDFRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDF 60

Query: 61  FFERQRVKKYINEIKIQTGCRN---GLVPSCRKRPAGEGEGSRRPVKAAPATNG------ 120
            F R+RVK+++NEI ++  C N   G+    RKR +   + ++ P  +    N       
Sbjct: 61  LFPRRRVKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSG 120

Query: 121 --KKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNF 180
             KKFRGVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF
Sbjct: 121 QIKKFRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNF 180

Query: 181 ATPP----------PPLPEKEVEAANIPSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEE 240
           + PP          P + EK V        + S   + E+  +LSSPTSVL  R+   ++
Sbjct: 181 SIPPQEEEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQ 240

Query: 241 PEKPQKSDEFQKP-PRAPLPPFVDDQFHECQGETSFSEEYYTDFPKFDDVFKVPSPDSPI 300
            ++P KS    KP P     P+    F+   GE+   + +  D P  D+ F   SP    
Sbjct: 241 VQQPFKS---AKPEPGVSNAPWWHTGFNTGLGES--DDSFPLDTPFLDNYFN-ESPPEMS 300

Query: 301 LFDE--QPLFLEESIWNEGFSEMFTNLPE--DFGSPLLSSSIGQGGDDY--FQDILMGSD 329
           +FD+    +F E    ++ F++M     E  +    L SSSI   G  +  F D L+ SD
Sbjct: 301 IFDQPMDQIFCEN---DDIFNDMLFLGGETMNIEDELTSSSIKDMGSTFSDFDDSLI-SD 353

BLAST of Lag0020086 vs. ExPASy Swiss-Prot
Match: Q9M374 (Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN=CRF6 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.7e-37
Identity = 101/209 (48.33%), Postives = 127/209 (60.77%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEE--GEFFERQRVKKYINE 68
           VKFTE+R  TN  ++  + SP  RLVRI+VTDP ATDSSSD++         RVK+Y+ E
Sbjct: 8   VKFTENRTVTNVAATPSNGSP--RLVRITVTDPFATDSSSDDDDNNNVTVVPRVKRYVKE 67

Query: 69  IKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAAEIRDPA 128
           I+   G  +    + RK    E E        + +   KK+RGVRQRPWGK+AAEIRDP+
Sbjct: 68  IRFCQG-ESSSSTAARKGKHKEEESVVVEDDVSTSVKPKKYRGVRQRPWGKFAAEIRDPS 127

Query: 129 RRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPLPEKEVEAANIPSVSGSY 188
            R R+WLGT+ TAEEAA+ YD AAI L+GP ALTNF TPP P P  +++         S 
Sbjct: 128 SRTRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLTPPTPTPVIDLQTV-------SA 187

Query: 189 YDSGEES-HNLSSPTSVLRFRTPSPEEPE 215
            D G +S  +L SPTSVLRF      E E
Sbjct: 188 CDYGRDSRQSLHSPTSVLRFNVNEETEHE 206

BLAST of Lag0020086 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.7e-36
Identity = 113/238 (47.48%), Postives = 140/238 (58.82%), Query Frame = 0

Query: 32  RLVRISVTDPDATDSSSDEEGEF------FERQRVKKYINEIKIQTGCRNGLVPSCRKRP 91
           R++RISVTDP ATDSSSDEE E        +R+RVKKY+ E+ + +   +   P  +KR 
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDKEKPMKKKR- 70

Query: 92  AGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMV 151
             +      PV    AT  +KFRGVRQRPWGKWAAEIRDP+RRVR+WLGT+DTAEEAA+V
Sbjct: 71  --KKRVVTVPVVVTTAT--RKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIV 130

Query: 152 YDNAAIQLRGPDALTNFATPPPPLPEKEVEAA-NIPSVSGSYYDSGEESHNLSSPTSVLR 211
           YDNAAIQLRGP+A  NF  PPPP+ E   EA+  +  VS      GE    L SP SVL 
Sbjct: 131 YDNAAIQLRGPNAELNF--PPPPVTENVEEASTEVKGVSDFIIGGGE---CLRSPVSVLE 190

Query: 212 FRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFHECQGETSFSEEYYTDFPKFDDVF 263
               SP   E     +EF     A +    +  F+     + FS   ++D    DDVF
Sbjct: 191 ----SPFSGESTAVKEEFVGVSTAEIVVKKEPSFN----GSDFSAPLFSD----DDVF 226

BLAST of Lag0020086 vs. ExPASy TrEMBL
Match: A0A0A0LB30 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.7e-154
Identity = 276/331 (83.38%), Postives = 298/331 (90.03%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           M+D+LF PVKFTEHRNFTNKFS+KKHH  E+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAA 120
           KKYINEIKIQ+GCRN L+PSCRKRPAG+    RR  K  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PLPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP P+PEKEVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFH 240
           PSVSGSYYDS EESHNLSSPTSVL FRTPSPEE EKP KSD+ QKPP     PFVDDQFH
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPP----APFVDDQFH 240

Query: 241 ECQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPED 300
           ECQGETSF+EEY+T+FP+FD  FK PSP++PI  D+QPLF E+SIWN+ FSE+FTNLPED
Sbjct: 241 ECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPED 300

Query: 301 FGSPLLSSSIGQGGDDYFQDILMGSDPLVVL 331
           FGSPLLSSSIGQGGDDYFQDILMGSDPLVVL
Sbjct: 301 FGSPLLSSSIGQGGDDYFQDILMGSDPLVVL 326

BLAST of Lag0020086 vs. ExPASy TrEMBL
Match: A0A5D3BJ86 (Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold63471G00010 PE=4 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 4.7e-151
Identity = 275/332 (82.83%), Postives = 295/332 (88.86%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           M+D+LF PVKFTEHRNFTNKFS+KKHH  E+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAA 120
           KKYINEIKIQ+GCRN L+PSCRKRPAG+    RR  K  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PLPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP  P+PEKEVE  NI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFH 240
           PSVSGSYYDS EESHNLSSPTSVL FRTPSPEE EKP KSD+ QKPP     PFVDDQFH
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPP----APFVDDQFH 240

Query: 241 ECQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPED 300
           ECQGETSFSEEY+T+FP+FD  FK PSP+SPI  D+QPLF E+SIWN+ FSE+FTNLPED
Sbjct: 241 ECQGETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNLPED 300

Query: 301 FGSPLL-SSSIGQGGDDYFQDILMGSDPLVVL 331
           FGSPLL SSSIGQGGDDYFQDIL GSDPLVVL
Sbjct: 301 FGSPLLSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of Lag0020086 vs. ExPASy TrEMBL
Match: A0A1S3AY55 (ethylene-responsive transcription factor CRF4-like OS=Cucumis melo OX=3656 GN=LOC103484149 PE=4 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 4.7e-151
Identity = 275/332 (82.83%), Postives = 295/332 (88.86%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           M+D+LF PVKFTEHRNFTNKFS+KKHH  E+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAA 120
           KKYINEIKIQ+GCRN L+PSCRKRPAG+    RR  K  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PLPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP  P+PEKEVE  NI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFH 240
           PSVSGSYYDS EESHNLSSPTSVL FRTPSPEE EKP KSD+ QKPP     PFVDDQFH
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPP----APFVDDQFH 240

Query: 241 ECQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPED 300
           ECQGETSFSEEY+T+FP+FD  FK PSP+SPI  D+QPLF E+SIWN+ FSE+FTNLPED
Sbjct: 241 ECQGETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNLPED 300

Query: 301 FGSPLL-SSSIGQGGDDYFQDILMGSDPLVVL 331
           FGSPLL SSSIGQGGDDYFQDIL GSDPLVVL
Sbjct: 301 FGSPLLSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of Lag0020086 vs. ExPASy TrEMBL
Match: A0A6J1G1E0 (ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662 GN=LOC111449782 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 6.7e-150
Identity = 270/330 (81.82%), Postives = 292/330 (88.48%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MDD+LF PVKFTEHR+FTNKFSS+K    +NR+VRI+VTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MDDTLFSPVKFTEHRSFTNKFSSRKQPPSQNRVVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAA 120
           K+Y+NEIKIQT  RN L+PS RKRPA EG  SRRPVKAAP  NGKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEIKIQTAPRNTLLPSARKRPAAEGSDSRRPVKAAPVMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPLPEKEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA PPPPLPE EVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFVDDQFHE 240
           SVSGSY DS EESHNLSSP+SVLRFRTPS EE EKPQ S  FQKPP  PLPP  D Q  E
Sbjct: 181 SVSGSYDDSCEESHNLSSPSSVLRFRTPSLEESEKPQNSSVFQKPPPPPLPPMADHQLRE 240

Query: 241 CQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFTNLPEDF 300
           CQGETSF EEYYTDFPKF+DVF++P  +SP+  D+QPL+LEESI+N+GFS+ FTNLP+DF
Sbjct: 241 CQGETSFYEEYYTDFPKFEDVFQLPFSESPMFLDDQPLYLEESIYNDGFSDWFTNLPDDF 300

Query: 301 GSPLLSSSIGQGGDDYFQDILMGSDPLVVL 331
           GSPLLSSSI Q G+DYFQDILMGSDPLVVL
Sbjct: 301 GSPLLSSSISQVGEDYFQDILMGSDPLVVL 330

BLAST of Lag0020086 vs. ExPASy TrEMBL
Match: A0A6J1CH70 (ethylene-responsive transcription factor CRF4-like OS=Momordica charantia OX=3673 GN=LOC111011618 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.1e-147
Identity = 271/336 (80.65%), Postives = 292/336 (86.90%), Query Frame = 0

Query: 1   MDDSLFFPVKFTEHRNFTNKFSSKK-----HHSPENRLVRISVTDPDATDSSSD-EEGEF 60
           M+D+LF PVKFTEHRNFT KFS+KK     HH PENR+VRISVTDPDATDSSSD EEGEF
Sbjct: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60

Query: 61  FERQRVKKYINEIKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRP 120
           FERQRVKKY+NEIKI++G RN LVP CRKRPAGEG   RRPVK  P TNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPLPEKEV 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATPPPP PEKE 
Sbjct: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPP-PEKEA 180

Query: 181 EAANIPSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEPEKPQKSDEFQKPPRAPLPPFV 240
           E  NIPSVSGSYYDSGEESHNLSSPTSVL FRT SPEE E P K + FQKP  +P P   
Sbjct: 181 E-VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKP--SP-PSLA 240

Query: 241 DDQFHECQGETSFSEEYYTDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSEMFT 300
           DDQFHECQGETSFS EY TDFPKF+D+F++PSPD  I FDEQPLF EESIWNEGFS++FT
Sbjct: 241 DDQFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFT 300

Query: 301 NLPEDFGSPLLSSSIGQGGDDYFQDILMGSDPLVVL 331
           N+PED GSP+LSSSI QGGDDYFQDIL+GSDPLVVL
Sbjct: 301 NMPEDLGSPVLSSSISQGGDDYFQDILLGSDPLVVL 331

BLAST of Lag0020086 vs. TAIR 10
Match: AT4G27950.1 (cytokinin response factor 4 )

HSP 1 Score: 200.7 bits (509), Expect = 1.9e-51
Identity = 142/336 (42.26%), Postives = 196/336 (58.33%), Query Frame = 0

Query: 8   PVKFTEH----RNFTNKFSSKKHHSPEN--RLVRISVTDPDATDSSSDEEGEFFERQRVK 67
           PVK+TEH    R +T K S ++  S  +  RLVR+S+TD DATDSSSDEE   F R+RVK
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVK 70

Query: 68  KYINEIKIQ-----TGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWG 127
           + INEI+++     TG  +      RKR   +    +  V      N KK+RGVRQRPWG
Sbjct: 71  RLINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKPSVS---GQNQKKYRGVRQRPWG 130

Query: 128 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPLPEKEVEA 187
           KWAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNF   P P P +E E 
Sbjct: 131 KWAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQ 190

Query: 188 ANIPSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEEP----EKPQKSDEFQKPPRAPLPP 247
               ++S S  +S ++S +LSSPTSVL ++T   EEP     KP K  EF +P + P+  
Sbjct: 191 EPESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVK-QEFLEPEQEPI-- 250

Query: 248 FVDDQFHECQGETSFSEEYY-TDFPKFDDVFKVPSPDSPILFDEQPLFLEESIWNEGFSE 307
                +H  +G T+ +++ +  D    D+ F    PD  I   +QP+   +   N+ F++
Sbjct: 251 ----SWHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFND 310

Query: 308 MF---TNLPEDFGSPL--LSSSIGQGGDDYFQDILM 323
           +    +N  E + S +  + SS     D    D+L+
Sbjct: 311 LMLFDSNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

BLAST of Lag0020086 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-50
Identity = 158/353 (44.76%), Postives = 201/353 (56.94%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGE-----FFERQRVKKY 68
           +KFTEH+  T    S+  ++ + R++RISVTDPDATDSSSD+E E       +R+RVKK+
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  INEI-----KIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAA--PATNGKKFRGVRQRPWG 128
           +NE+      + TG    +    R++ A + E +  PV +A    T  KKFRGVRQRPWG
Sbjct: 72  VNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWG 131

Query: 129 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA-TP--------PP 188
           KWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+ TP        PP
Sbjct: 132 KWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPP 191

Query: 189 PLPEKEVEAAN---IPSVSGSYYDSGEESHN-LSSPTSVLRFRTPSPEEPEKPQKSDEFQ 248
           P P K+ +  N     SV+ S   S   S++ L SP SVLR           P   DEF 
Sbjct: 192 PSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLR----------SPFAVDEFS 251

Query: 249 KPPRAPLPPFVDDQFHECQGETSFSEEYYTDF--PKF--DDVFKVPSPDSPILFDEQPLF 308
               +P+   V     E    T+ SE  ++DF  P F  DDVF   S   P       LF
Sbjct: 252 GISSSPVAAVV---VKEEPSMTTVSET-FSDFSAPLFSDDDVFDFRSSVVPDYLGGD-LF 311

Query: 309 LEESIWNEGFSEMFTNLPEDFGSPLLSSSIGQGGDDYFQDI--LMGSDPLVVL 331
            E+    +  ++M  N   DFGS L S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 GEDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of Lag0020086 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-50
Identity = 158/353 (44.76%), Postives = 201/353 (56.94%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGE-----FFERQRVKKY 68
           +KFTEH+  T    S+  ++ + R++RISVTDPDATDSSSD+E E       +R+RVKK+
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  INEI-----KIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAA--PATNGKKFRGVRQRPWG 128
           +NE+      + TG    +    R++ A + E +  PV +A    T  KKFRGVRQRPWG
Sbjct: 72  VNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWG 131

Query: 129 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA-TP--------PP 188
           KWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+ TP        PP
Sbjct: 132 KWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPP 191

Query: 189 PLPEKEVEAAN---IPSVSGSYYDSGEESHN-LSSPTSVLRFRTPSPEEPEKPQKSDEFQ 248
           P P K+ +  N     SV+ S   S   S++ L SP SVLR           P   DEF 
Sbjct: 192 PSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLR----------SPFAVDEFS 251

Query: 249 KPPRAPLPPFVDDQFHECQGETSFSEEYYTDF--PKF--DDVFKVPSPDSPILFDEQPLF 308
               +P+   V     E    T+ SE  ++DF  P F  DDVF   S   P       LF
Sbjct: 252 GISSSPVAAVV---VKEEPSMTTVSET-FSDFSAPLFSDDDVFDFRSSVVPDYLGGD-LF 311

Query: 309 LEESIWNEGFSEMFTNLPEDFGSPLLSSSIGQGGDDYFQDI--LMGSDPLVVL 331
            E+    +  ++M  N   DFGS L S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 GEDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of Lag0020086 vs. TAIR 10
Match: AT5G53290.1 (cytokinin response factor 3 )

HSP 1 Score: 187.6 bits (475), Expect = 1.6e-47
Identity = 146/363 (40.22%), Postives = 199/363 (54.82%), Query Frame = 0

Query: 1   MDDSL-FFPVKFTEHR----NFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEEGE-- 60
           MD+ + F P+K+TEH+     +T K S K       + V I  TDPDATDSSSDE+ E  
Sbjct: 1   MDEYIDFRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDF 60

Query: 61  FFERQRVKKYINEIKIQTGCRN---GLVPSCRKRPAGEGEGSRRPVKAAPATNG------ 120
            F R+RVK+++NEI ++  C N   G+    RKR +   + ++ P  +    N       
Sbjct: 61  LFPRRRVKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSG 120

Query: 121 --KKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNF 180
             KKFRGVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF
Sbjct: 121 QIKKFRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNF 180

Query: 181 ATPP----------PPLPEKEVEAANIPSVSGSYYDSGEESHNLSSPTSVLRFRTPSPEE 240
           + PP          P + EK V        + S   + E+  +LSSPTSVL  R+   ++
Sbjct: 181 SIPPQEEEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQ 240

Query: 241 PEKPQKSDEFQKP-PRAPLPPFVDDQFHECQGETSFSEEYYTDFPKFDDVFKVPSPDSPI 300
            ++P KS    KP P     P+    F+   GE+   + +  D P  D+ F   SP    
Sbjct: 241 VQQPFKS---AKPEPGVSNAPWWHTGFNTGLGES--DDSFPLDTPFLDNYFN-ESPPEMS 300

Query: 301 LFDE--QPLFLEESIWNEGFSEMFTNLPE--DFGSPLLSSSIGQGGDDY--FQDILMGSD 329
           +FD+    +F E    ++ F++M     E  +    L SSSI   G  +  F D L+ SD
Sbjct: 301 IFDQPMDQIFCEN---DDIFNDMLFLGGETMNIEDELTSSSIKDMGSTFSDFDDSLI-SD 353

BLAST of Lag0020086 vs. TAIR 10
Match: AT3G61630.1 (cytokinin response factor 6 )

HSP 1 Score: 156.4 bits (394), Expect = 4.0e-38
Identity = 101/209 (48.33%), Postives = 127/209 (60.77%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSSKKHHSPENRLVRISVTDPDATDSSSDEE--GEFFERQRVKKYINE 68
           VKFTE+R  TN  ++  + SP  RLVRI+VTDP ATDSSSD++         RVK+Y+ E
Sbjct: 8   VKFTENRTVTNVAATPSNGSP--RLVRITVTDPFATDSSSDDDDNNNVTVVPRVKRYVKE 67

Query: 69  IKIQTGCRNGLVPSCRKRPAGEGEGSRRPVKAAPATNGKKFRGVRQRPWGKWAAEIRDPA 128
           I+   G  +    + RK    E E        + +   KK+RGVRQRPWGK+AAEIRDP+
Sbjct: 68  IRFCQG-ESSSSTAARKGKHKEEESVVVEDDVSTSVKPKKYRGVRQRPWGKFAAEIRDPS 127

Query: 129 RRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPLPEKEVEAANIPSVSGSY 188
            R R+WLGT+ TAEEAA+ YD AAI L+GP ALTNF TPP P P  +++         S 
Sbjct: 128 SRTRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLTPPTPTPVIDLQTV-------SA 187

Query: 189 YDSGEES-HNLSSPTSVLRFRTPSPEEPE 215
            D G +S  +L SPTSVLRF      E E
Sbjct: 188 CDYGRDSRQSLHSPTSVLRFNVNEETEHE 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004149454.11.6e-15383.38ethylene-responsive transcription factor CRF4 [Cucumis sativus] >KGN57872.1 hypo... [more]
KAG7013289.18.7e-15282.42Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma s... [more]
XP_008439327.19.6e-15182.83PREDICTED: ethylene-responsive transcription factor CRF4-like [Cucumis melo] >KA... [more]
XP_038900136.11.6e-15082.78ethylene-responsive transcription factor CRF4-like [Benincasa hispida][more]
XP_022945589.11.4e-14981.82ethylene-responsive transcription factor CRF4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SUE32.6e-5042.26Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUQ22.9e-4944.76Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FK122.3e-4640.22Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9M3745.7e-3748.33Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN... [more]
O825031.7e-3647.48Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0LB307.7e-15483.38AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=... [more]
A0A5D3BJ864.7e-15182.83Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa O... [more]
A0A1S3AY554.7e-15182.83ethylene-responsive transcription factor CRF4-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1G1E06.7e-15081.82ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1CH703.1e-14780.65ethylene-responsive transcription factor CRF4-like OS=Momordica charantia OX=367... [more]
Match NameE-valueIdentityDescription
AT4G27950.11.9e-5142.26cytokinin response factor 4 [more]
AT4G23750.12.1e-5044.76cytokinin response factor 2 [more]
AT4G23750.22.1e-5044.76cytokinin response factor 2 [more]
AT5G53290.11.6e-4740.22cytokinin response factor 3 [more]
AT3G61630.14.0e-3848.33cytokinin response factor 6 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 129..145
score: 61.5
coord: 107..118
score: 70.91
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 106..169
e-value: 1.7E-38
score: 143.9
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 106..155
e-value: 8.5E-14
score: 51.5
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 106..163
score: 23.762146
IPR001471AP2/ERF domainCDDcd00018AP2coord: 105..163
e-value: 8.35557E-34
score: 116.987
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 105..164
e-value: 3.8E-26
score: 93.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..52
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 208..222
NoneNo IPR availablePANTHERPTHR31194:SF137ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF4-LIKEcoord: 6..217
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 6..217
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 106..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020086.1Lag0020086.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity