Lag0020072 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0020072 vs. NCBI nr
Match: XP_038902999.1 (probable membrane-associated kinase regulator 5 [Benincasa hispida])

HSP 1 Score: 510.0 bits (1312), Expect = 1.6e-140
Identity = 283/338 (83.73%), Postives = 296/338 (87.57%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFISHPHSETGKSPDE 60
           MEALDFVKLHNLY+HFSSIVETAYPLIRSHFYGDDQ+EDSFFDLDFIS P SET KSPDE
Sbjct: 1   MEALDFVKLHNLYSHFSSIVETAYPLIRSHFYGDDQDEDSFFDLDFISPPPSETCKSPDE 60

Query: 61  KSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
            +LTHSG+NELCF+QRN LLSQSDLISK KILPIELSSKPESPVSVSILKSAP+LSISLF
Sbjct: 61  NNLTHSGQNELCFSQRNLLLSQSDLISKLKILPIELSSKPESPVSVSILKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQKT+E +  S   KL STESKRFAFKLNRV+SSRN+NRIDASERQSKRFSGE
Sbjct: 121 KKPKLMAKQKTEEKEPPSL--KLHSTESKRFAFKLNRVNSSRNNNRIDASERQSKRFSGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSP-TTTTATSIDEKQRNIAIGIRVVCRRLGKS 240
           GMQKYLKLIKPLYVKVSRK NYR SPVSSP TTTTATSIDEKQRNIAIGIRVVCRRLGKS
Sbjct: 181 GMQKYLKLIKPLYVKVSRKQNYRSSPVSSPSTTTTATSIDEKQRNIAIGIRVVCRRLGKS 240

Query: 241 KSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIISI 300
           KSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR                 
Sbjct: 241 KSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR----------------- 300

Query: 301 EISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGEQ 338
              DD+DGTCDDNLFG GDLQ +K GKD  SEAK+ EQ
Sbjct: 301 ---DDIDGTCDDNLFGAGDLQNQKTGKDSFSEAKQDEQ 316

BLAST of Lag0020072 vs. NCBI nr
Match: XP_023541341.1 (probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 498.0 bits (1281), Expect = 6.2e-137
Identity = 278/336 (82.74%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFISHPHSETGKSPDE 60
           MEALDFVKLHNLYAHFSSIVETAYPLIRS  YGDDQ+EDSFFDLDFIS P S +G+SPD+
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRLYGDDQDEDSFFDLDFISPPSSGSGESPDQ 60

Query: 61  KSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
            +L+ SGENELCF+QRN LLSQSDLISKRKILPIELSSKPESPV VSI KSAP+LSISLF
Sbjct: 61  SNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+T+ETDS SS  KLQSTESKRF FKLNRV+SSRNSNR DASERQSKRF GE
Sbjct: 121 KKPKLMAKQRTEETDSLSSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSK 240
            MQKYLKLIKPLYVKVSRK NY  S  SSP TTTATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 AMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIISIE 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR                  
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR------------------ 300

Query: 301 ISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGE 337
             DDVDGTCDDN FGTGDLQKEKAGKDV SEAKEGE
Sbjct: 301 --DDVDGTCDDNQFGTGDLQKEKAGKDVFSEAKEGE 315

BLAST of Lag0020072 vs. NCBI nr
Match: KAG7014738.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 498.0 bits (1281), Expect = 6.2e-137
Identity = 282/338 (83.43%), Postives = 297/338 (87.87%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFISHPHSETGKSPDE 60
           MEALDFVKLHNLYAHFSSIVETAY L RS    D+ +EDSFFDLDF+S P SETGKSPDE
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYNLNRS----DNHDEDSFFDLDFVSLPTSETGKSPDE 60

Query: 61  KSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           K+LT SGENELCF QRN L SQSDLISKRKILPIELSSKPESPVSVSILKSAP+LSISLF
Sbjct: 61  KNLTRSGENELCFPQRNLLRSQSDLISKRKILPIELSSKPESPVSVSILKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQKTDET+SSS   KLQ+TESKRFAFKLNR++SSRN+NRIDASERQSKRFSGE
Sbjct: 121 KKPKLMAKQKTDETESSSL--KLQNTESKRFAFKLNRLNSSRNNNRIDASERQSKRFSGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSK 240
           G+QKYLKLIKPLYVKVSRK NYR SPVSSP     TSIDEKQRNIAIGIRVVCRRLGKSK
Sbjct: 181 GIQKYLKLIKPLYVKVSRKQNYRSSPVSSP----VTSIDEKQRNIAIGIRVVCRRLGKSK 240

Query: 241 -SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIISI 300
            SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSA+RGKIQ QK   +      
Sbjct: 241 SSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSASRGKIQKQKTRTY------ 300

Query: 301 EISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGEQ 338
               DVDGTCDDNLFGTGDLQK+KAG D  SEAK+GEQ
Sbjct: 301 ----DVDGTCDDNLFGTGDLQKQKAGTDAFSEAKQGEQ 318

BLAST of Lag0020072 vs. NCBI nr
Match: KAG6574213.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 496.1 bits (1276), Expect = 2.4e-136
Identity = 277/336 (82.44%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFISHPHSETGKSPDE 60
           MEALDFVKLHNLYAHFSSIVETAYPLIRS FYGDDQ+EDSFFDLDFIS   S +G+SPD+
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  KSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           ++L+ SGE +LCF+QRN LLSQSDLISKRKILPIELSSKPESPV VSI KSAP+LSISLF
Sbjct: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+TDETDS  S  KLQSTESKRF FKLNRV+SSRNSNR DASERQSKRF GE
Sbjct: 121 KKPKLMAKQRTDETDSPLSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSK 240
           GMQKYLKLIKPLYVKVSRK NY  S  SSP TTTATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 GMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIISIE 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR                  
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR------------------ 300

Query: 301 ISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGE 337
             DDVDGTCDDN FGTGDLQKEKAGKDV SEAKEGE
Sbjct: 301 --DDVDGTCDDNQFGTGDLQKEKAGKDVFSEAKEGE 315

BLAST of Lag0020072 vs. NCBI nr
Match: XP_022945795.1 (probable membrane-associated kinase regulator 5 [Cucurbita moschata])

HSP 1 Score: 496.1 bits (1276), Expect = 2.4e-136
Identity = 277/336 (82.44%), Postives = 289/336 (86.01%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFISHPHSETGKSPDE 60
           MEALDFVK HNLYAHFSSIVETAYPLIRS FYGDDQ+EDSFFDLDFIS   S +G+SPD+
Sbjct: 1   MEALDFVKFHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  KSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           ++L+ SGENELCF+QRN LLSQSDLISKRKILPIELSSKPESPV VSI KSAP+LSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+TDETDS  S  KLQSTESKRF FKLNRV+SSRNSNR DASE QSKRF GE
Sbjct: 121 KKPKLMAKQRTDETDSPLSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSK 240
           GMQKYLKLIKPLYVKVSRK NY  S  SSP TTTATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 GMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIISIE 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR                  
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR------------------ 300

Query: 301 ISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGE 337
             DDVDGTCDDN FGTGDLQKEKAGKDV SEAKEGE
Sbjct: 301 --DDVDGTCDDNQFGTGDLQKEKAGKDVFSEAKEGE 315

BLAST of Lag0020072 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 7.9e-18
Identity = 87/248 (35.08%), Postives = 128/248 (51.61%), Query Frame = 0

Query: 38  EDSFFDLDFISHPHSETGKSPDEKSLTHSGENELCFAQRNRLLSQSDLISKRKILP-IEL 97
           EDSFF+L           K+P EK    +      ++  N          K K+LP +++
Sbjct: 47  EDSFFELKI----SLSDFKTPKEKQRLETTTTTTTYSVSN----------KSKVLPFVDI 106

Query: 98  SSKPESPVSVSILKSAPKLSISLFKK-PKLMAKQKTDETDSSSSSPKLQSTESKRFAFKL 157
           SSKP+     ++LKS  K     FKK  K    +K  E ++ +S       + +      
Sbjct: 107 SSKPQQS-PTTLLKSGQKFRAFSFKKSEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSF 166

Query: 158 NRVSSSRNSNRIDASERQSKRFSGEGMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTA 217
            + +S   S + D        F     +++  LIKPLY K ++K +   S ++SPT++ A
Sbjct: 167 RKTASIARSQQTD-----DTMFDDSVSKRFFSLIKPLYTKSTKKQS--SSTITSPTSSPA 226

Query: 218 TSIDEKQR-NIAIGIRVVCRRLGKSKSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHC 277
           T   EKQR NI  G+R V R+LGKS+S+S+A G  +SP NR D+SL  Q DGI+SAILHC
Sbjct: 227 TR--EKQRSNIPSGMRSVRRQLGKSRSASAAIG-GMSPANRIDESLQVQQDGIQSAILHC 269

Query: 278 KRSFSATR 283
           K+SF  +R
Sbjct: 287 KKSFHGSR 269

BLAST of Lag0020072 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 9.7e-16
Identity = 80/227 (35.24%), Postives = 113/227 (49.78%), Query Frame = 0

Query: 83  SDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLFKKPKLMAKQKTDETDSSSSSPK 142
           S+ + K+   P  LS  PES       KS   +++ + + P +    + + + +SSSS  
Sbjct: 185 SEDLDKQTHPPPPLSLSPESQ-----QKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSS 244

Query: 143 LQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGEGMQKYLKLIKPLYVKVSRKHNY 202
             S+ S          SS +  N  ++     KRF    MQKYLK +KPLY++VSR++  
Sbjct: 245 SSSSSS----------SSLKKQNGNESVVSDEKRF--VMMQKYLKKVKPLYIRVSRRYGE 304

Query: 203 R---------PS-------PVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSKSSSS-- 262
           +         PS          SP  T   +      NI  G++VV + LGKS+SSSS  
Sbjct: 305 KLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTA 364

Query: 263 -------ATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGK 285
                  AT    S + RRDDSLLQQ D I+SAILHCKRSF+++R K
Sbjct: 365 TTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

BLAST of Lag0020072 vs. ExPASy TrEMBL
Match: A0A6J1G1Z9 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111449936 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.1e-136
Identity = 277/336 (82.44%), Postives = 289/336 (86.01%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFISHPHSETGKSPDE 60
           MEALDFVK HNLYAHFSSIVETAYPLIRS FYGDDQ+EDSFFDLDFIS   S +G+SPD+
Sbjct: 1   MEALDFVKFHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  KSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           ++L+ SGENELCF+QRN LLSQSDLISKRKILPIELSSKPESPV VSI KSAP+LSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+TDETDS  S  KLQSTESKRF FKLNRV+SSRNSNR DASE QSKRF GE
Sbjct: 121 KKPKLMAKQRTDETDSPLSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSK 240
           GMQKYLKLIKPLYVKVSRK NY  S  SSP TTTATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 GMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIISIE 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR                  
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR------------------ 300

Query: 301 ISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGE 337
             DDVDGTCDDN FGTGDLQKEKAGKDV SEAKEGE
Sbjct: 301 --DDVDGTCDDNQFGTGDLQKEKAGKDVFSEAKEGE 315

BLAST of Lag0020072 vs. ExPASy TrEMBL
Match: A0A6J1J4Q0 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111482618 PE=4 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 7.4e-136
Identity = 274/335 (81.79%), Postives = 290/335 (86.57%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFISHPHSETGKSPDE 60
           MEALDFVKLHNLYAHFSSIVETAY L RSHF+GD+ +EDSFFDLDF+S P SETGKSPDE
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYNLNRSHFHGDNHDEDSFFDLDFVSLPTSETGKSPDE 60

Query: 61  KSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           K+LT S ENELCF QRN LLSQSDLISKRKILPIELSSKPESPVSVS+LKSAP+LSISLF
Sbjct: 61  KNLTRSSENELCFTQRNLLLSQSDLISKRKILPIELSSKPESPVSVSVLKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQKTDET+ SS   KLQSTESKRFAFKLNR++SSRN+NRIDASERQSKRFSGE
Sbjct: 121 KKPKLMAKQKTDETEPSSL--KLQSTESKRFAFKLNRLNSSRNNNRIDASERQSKRFSGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSK 240
           G+QKYLKLIKPLYVKVSRK NYR SPVSSP     TSIDEKQRNIAIGIRVVCRRLG+SK
Sbjct: 181 GIQKYLKLIKPLYVKVSRKQNYRSSPVSSP----VTSIDEKQRNIAIGIRVVCRRLGRSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIISIE 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSA+R                  
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSASR------------------ 300

Query: 301 ISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEG 336
             DDVDGTCDDNL GTGDLQK+K G D  SEAK+G
Sbjct: 301 --DDVDGTCDDNLSGTGDLQKQKTGTDAFSEAKQG 309

BLAST of Lag0020072 vs. ExPASy TrEMBL
Match: A0A6J1HUJ8 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111467591 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 9.7e-136
Identity = 276/336 (82.14%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFISHPHSETGKSPDE 60
           MEALDFVKLHNLYA+FSSIVETAYPLIRS  YGDDQ+EDSFFDLDFIS P S +G+SPD 
Sbjct: 1   MEALDFVKLHNLYAYFSSIVETAYPLIRSRIYGDDQDEDSFFDLDFISPPSSGSGESPDR 60

Query: 61  KSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           ++L+ SGENELCF+QRN LLSQSDLISKRKILPIELSSKPESPV VSI KSA +LSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSASRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+TDETD+ SS  KLQSTESKRF FKLNRV+SSRNSNR DASERQSKRF GE
Sbjct: 121 KKPKLMAKQRTDETDTPSSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSK 240
           GM+KYLKLIKPLYVKVSRK NY  SPVSSP TTTATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 GMRKYLKLIKPLYVKVSRKPNYSSSPVSSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIISIE 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR                  
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR------------------ 300

Query: 301 ISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGE 337
             DDVDGTCDDN FGT DLQKEKA KDV SEAKEGE
Sbjct: 301 --DDVDGTCDDNQFGTDDLQKEKARKDVFSEAKEGE 315

BLAST of Lag0020072 vs. ExPASy TrEMBL
Match: A0A6J1EAG2 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111430826 PE=4 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.1e-134
Identity = 276/337 (81.90%), Postives = 291/337 (86.35%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFISHPHSETGKSPDE 60
           MEALDFVKLHNLYAHFSSIVETAY L RS    D+ +EDSFFDLDF+S P SETGKSPDE
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYNLNRS----DNHDEDSFFDLDFVSLPTSETGKSPDE 60

Query: 61  KSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           K+LT SGENELCF QRN L SQSDLISKRKILPIELSSKPESPVSVSILKSAP+LSISLF
Sbjct: 61  KNLTRSGENELCFPQRNLLRSQSDLISKRKILPIELSSKPESPVSVSILKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQKTDET+S+S   KLQ+TESKRFAFKLNR++SSRN+NRIDASERQSKRFSGE
Sbjct: 121 KKPKLMAKQKTDETESASL--KLQNTESKRFAFKLNRLNSSRNNNRIDASERQSKRFSGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSK 240
           G+QKYLKLIKPLYVKVSRK NYR SPVSSP     TSIDEKQRNIAIGIRVVCRRLGKSK
Sbjct: 181 GIQKYLKLIKPLYVKVSRKQNYRSSPVSSP----VTSIDEKQRNIAIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIISIE 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSA+R                  
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSASR------------------ 300

Query: 301 ISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGEQ 338
             DDVDGTCDDNLFGTGDLQK+KAG D  SEAK+GEQ
Sbjct: 301 --DDVDGTCDDNLFGTGDLQKQKAGTDAFSEAKQGEQ 307

BLAST of Lag0020072 vs. ExPASy TrEMBL
Match: A0A0A0L6R5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184590 PE=4 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.5e-133
Identity = 276/339 (81.42%), Postives = 291/339 (85.84%), Query Frame = 0

Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDFIS-HPHSETGKSPD 60
           MEALDFVKLHNLY+HFSSIVETAYP IRSHFYGDDQ+EDSFFDLDFIS  P SETGKSPD
Sbjct: 1   MEALDFVKLHNLYSHFSSIVETAYPFIRSHFYGDDQDEDSFFDLDFISLPPSSETGKSPD 60

Query: 61  EKSLTHSGENELCFAQRNRLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISL 120
           EK+LTHS +NEL F+QRN LLS SDLISKRKILPIELSSKPESPVSVSILKSAP+LSISL
Sbjct: 61  EKNLTHSSQNELGFSQRNLLLSHSDLISKRKILPIELSSKPESPVSVSILKSAPRLSISL 120

Query: 121 FKKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSG 180
           FKKPKLMAKQK DETDS S   KLQSTESKRF FKLNRV+SSRN+NRIDA+ERQSKR   
Sbjct: 121 FKKPKLMAKQKIDETDSPSH--KLQSTESKRFTFKLNRVNSSRNNNRIDATERQSKR--- 180

Query: 181 EGMQKYLKLIKPLYVKVSRKHNYRPSPVSSP-TTTTATSIDEKQRNIAIGIRVVCRRLGK 240
           EGMQKYLKLIKPLYVKVSRK N+R SPVSSP TTTTA SIDEKQRN AIGIRVVC+RLGK
Sbjct: 181 EGMQKYLKLIKPLYVKVSRKQNHRSSPVSSPSTTTTAPSIDEKQRNSAIGIRVVCKRLGK 240

Query: 241 SKSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNQKNSNHLTIIS 300
           SKSSSS+TGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR                
Sbjct: 241 SKSSSSSTGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR---------------- 300

Query: 301 IEISDDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGEQ 338
               DDVDGTCDDN+F T DLQK+K GKD  SEAK+ EQ
Sbjct: 301 ---DDDVDGTCDDNVFVTEDLQKQKTGKDSFSEAKQDEQ 315

BLAST of Lag0020072 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 92.8 bits (229), Expect = 5.6e-19
Identity = 87/248 (35.08%), Postives = 128/248 (51.61%), Query Frame = 0

Query: 38  EDSFFDLDFISHPHSETGKSPDEKSLTHSGENELCFAQRNRLLSQSDLISKRKILP-IEL 97
           EDSFF+L           K+P EK    +      ++  N          K K+LP +++
Sbjct: 47  EDSFFELKI----SLSDFKTPKEKQRLETTTTTTTYSVSN----------KSKVLPFVDI 106

Query: 98  SSKPESPVSVSILKSAPKLSISLFKK-PKLMAKQKTDETDSSSSSPKLQSTESKRFAFKL 157
           SSKP+     ++LKS  K     FKK  K    +K  E ++ +S       + +      
Sbjct: 107 SSKPQQS-PTTLLKSGQKFRAFSFKKSEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSF 166

Query: 158 NRVSSSRNSNRIDASERQSKRFSGEGMQKYLKLIKPLYVKVSRKHNYRPSPVSSPTTTTA 217
            + +S   S + D        F     +++  LIKPLY K ++K +   S ++SPT++ A
Sbjct: 167 RKTASIARSQQTD-----DTMFDDSVSKRFFSLIKPLYTKSTKKQS--SSTITSPTSSPA 226

Query: 218 TSIDEKQR-NIAIGIRVVCRRLGKSKSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHC 277
           T   EKQR NI  G+R V R+LGKS+S+S+A G  +SP NR D+SL  Q DGI+SAILHC
Sbjct: 227 TR--EKQRSNIPSGMRSVRRQLGKSRSASAAIG-GMSPANRIDESLQVQQDGIQSAILHC 269

Query: 278 KRSFSATR 283
           K+SF  +R
Sbjct: 287 KKSFHGSR 269

BLAST of Lag0020072 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 85.9 bits (211), Expect = 6.9e-17
Identity = 80/227 (35.24%), Postives = 113/227 (49.78%), Query Frame = 0

Query: 83  SDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLFKKPKLMAKQKTDETDSSSSSPK 142
           S+ + K+   P  LS  PES       KS   +++ + + P +    + + + +SSSS  
Sbjct: 185 SEDLDKQTHPPPPLSLSPESQ-----QKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSS 244

Query: 143 LQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGEGMQKYLKLIKPLYVKVSRKHNY 202
             S+ S          SS +  N  ++     KRF    MQKYLK +KPLY++VSR++  
Sbjct: 245 SSSSSS----------SSLKKQNGNESVVSDEKRF--VMMQKYLKKVKPLYIRVSRRYGE 304

Query: 203 R---------PS-------PVSSPTTTTATSIDEKQRNIAIGIRVVCRRLGKSKSSSS-- 262
           +         PS          SP  T   +      NI  G++VV + LGKS+SSSS  
Sbjct: 305 KLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTA 364

Query: 263 -------ATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGK 285
                  AT    S + RRDDSLLQQ D I+SAILHCKRSF+++R K
Sbjct: 365 TTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394