Lag0020068 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020068
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionNADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
Locationchr5: 47824602 .. 47825876 (+)
RNA-Seq ExpressionLag0020068
SyntenyLag0020068
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTCTGCGAGTGATCGGACGGCCATTACTGGCCAGGGTGAAGCAAACGACGGGGATCGTCGGTCTGGATGTCGTCCCCAACGCCCGAGAGGTCTTGATCGGCCTTTACAACAAAACCCTAAAGGAGATCCAGGCCGTTCCCGAGGACGAAGGATACCGTAAGGCGGTCGAGAGCTTCACACGCCACCGCTTGAAGGTCTGCCAGGAGGAAGAGGACTGGGAGCAAATTGAGAAGCGGCTTGGCTGTGGTCAGGTCGAGGAGCTCATCGAGGAGGCTCAGGATGAGCTCACGCTCATTGGGAAAATGATCGGTACGTATATAAATTTATGTAACCAAAGTTCTATCTTTACGTATTTGTGAAAATTTGACGTACAGTCGAGTTCCATTACATTTTTTTCCCTGTCTGTTTCGGTTTAGGAGGGGAATGTATGAATTGGAGATTCAAGTTAGCAGGATTTTCGAGTTATATGATATTTATATTATTGCATTACGTGGAAATGATGAAAACCGTACTTTTCCTGCTCAAATTTCTAAGGAAAAATCGTGACTGCGAATTTTAGAAATGTTGGCTGTTATGAGTCTGATATCGTTCATGAGAACTCGTAGGGAAGAGAACGGAGGTTCATGATTTCTCTAGTACTGAGCGTCAAAGAATGGCATTTGCATCTTGTAGCACTGGAAAAAACTGGGTTGAGACTCGGAATTAGACTGTAATGATCCTTCTGCCGTTGTTAGCTTCTTACCTCGTTGAATAAAATGATGAGCCTCATGGCCTAATAGCAATCCCTGATTTGTTTTCGTCTCTTCACCCGATTCGTGTCTCTGAACTTTTGAAATTGATCTTCTATTCTAACTGAGGTGGATGAAAGTTACATATTTCGACATTAGTTTAACATATCAAGTTGAAGCATCATGAAGGAGGAAATATATGTTTGTATTTGGTCAAGTAATAGGCCTGACTCTTCTGGTCTTGTTGCATTGGTGCATGCTCATTATTTCAAAGATGGAGCTTTATGAAGTTTTTTCAGTCATCACAATCTTTTTGTGGTTTTGTTAGTGATTCGTTATCTGAAATATGCAGAGTGGGATCCCTGGGGTGTTCCTGATGACTACGAATGTGAAGTGGTCGAGAACGATGCTCCGGTACCCAAGCACATTCCTCTACATCGACCAGGCCCTCTCCCTGAGGAGTTTTACAAAACGCTGGAGGCCATTAGTGGTGGTAGTGCACAAAAAGTAGAGACGGCCGAGAAAGCATCGGTGACAGAGTGA

mRNA sequence

ATGTTTCTGCGAGTGATCGGACGGCCATTACTGGCCAGGGTGAAGCAAACGACGGGGATCGTCGGTCTGGATGTCGTCCCCAACGCCCGAGAGGTCTTGATCGGCCTTTACAACAAAACCCTAAAGGAGATCCAGGCCGTTCCCGAGGACGAAGGATACCGTAAGGCGGTCGAGAGCTTCACACGCCACCGCTTGAAGGTCTGCCAGGAGGAAGAGGACTGGGAGCAAATTGAGAAGCGGCTTGGCTGTGGTCAGGTCGAGGAGCTCATCGAGGAGGCTCAGGATGAGCTCACGCTCATTGGGAAAATGATCGAGTGGGATCCCTGGGGTGTTCCTGATGACTACGAATGTGAAGTGGTCGAGAACGATGCTCCGGTACCCAAGCACATTCCTCTACATCGACCAGGCCCTCTCCCTGAGGAGTTTTACAAAACGCTGGAGGCCATTAGTGGTGGTAGTGCACAAAAAGTAGAGACGGCCGAGAAAGCATCGGTGACAGAGTGA

Coding sequence (CDS)

ATGTTTCTGCGAGTGATCGGACGGCCATTACTGGCCAGGGTGAAGCAAACGACGGGGATCGTCGGTCTGGATGTCGTCCCCAACGCCCGAGAGGTCTTGATCGGCCTTTACAACAAAACCCTAAAGGAGATCCAGGCCGTTCCCGAGGACGAAGGATACCGTAAGGCGGTCGAGAGCTTCACACGCCACCGCTTGAAGGTCTGCCAGGAGGAAGAGGACTGGGAGCAAATTGAGAAGCGGCTTGGCTGTGGTCAGGTCGAGGAGCTCATCGAGGAGGCTCAGGATGAGCTCACGCTCATTGGGAAAATGATCGAGTGGGATCCCTGGGGTGTTCCTGATGACTACGAATGTGAAGTGGTCGAGAACGATGCTCCGGTACCCAAGCACATTCCTCTACATCGACCAGGCCCTCTCCCTGAGGAGTTTTACAAAACGCTGGAGGCCATTAGTGGTGGTAGTGCACAAAAAGTAGAGACGGCCGAGAAAGCATCGGTGACAGAGTGA

Protein sequence

MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPWGVPDDYECEVVENDAPVPKHIPLHRPGPLPEEFYKTLEAISGGSAQKVETAEKASVTE
Homology
[Close]
BLAST of Lag0020068 vs. NCBI nr
Match: XP_022945797.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita moschata] >XP_023542504.1 probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.0 bits (811), Expect = 9.7e-83
Identity = 159/169 (94.08%), Postives = 164/169 (97.04%), Query Frame = 0 

Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPWGVPDDYECEVV 120
           TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDEL LI KMIEWDPWGVPDDYECEV+
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAI-SGGSAQKVETAEKAS-VTE 168
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAI SGGSAQKVETA+KAS VTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISSGGSAQKVETADKASQVTE 169

[Close]
BLAST of Lag0020068 vs. NCBI nr
Match: XP_004140748.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucumis sativus] >KGN57450.2 hypothetical protein Csa_010484 [Cucumis sativus])

HSP 1 Score: 316.2 bits (809), Expect = 1.7e-82
Identity = 157/168 (93.45%), Postives = 161/168 (95.83%), Query Frame = 0 

Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPWGVPDDYECEVV 120
           TRHRLKVCQEEEDWE IEKRLGCGQVEELIEEAQDEL LIGKMIEWDPWGVPDDYECEV+
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGGSAQKVETAEKAS-VTE 168
           ENDAPVPKHIPLHRPGPLPEEFYKTLEAISG S +KVET EKAS VTE
Sbjct: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 168

[Close]
BLAST of Lag0020068 vs. NCBI nr
Match: KAG6574208.1 (putative NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013272.1 putative NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 315.5 bits (807), Expect = 2.8e-82
Identity = 158/169 (93.49%), Postives = 163/169 (96.45%), Query Frame = 0 

Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPWGVPDDYECEVV 120
           TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDEL LI KMIEWDPWGVPDDYECEV+
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAI-SGGSAQKVETAEKAS-VTE 168
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAI SGGSAQKVET +KAS VTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISSGGSAQKVETTDKASQVTE 169

[Close]
BLAST of Lag0020068 vs. NCBI nr
Match: XP_022141236.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Momordica charantia])

HSP 1 Score: 315.1 bits (806), Expect = 3.7e-82
Identity = 156/168 (92.86%), Postives = 163/168 (97.02%), Query Frame = 0 

Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGL+VVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPWGVPDDYECEVV 120
           TRHRLKVCQEE+DWE+IEKRLGCGQVEELIEEAQDEL LI KMIEWDPWGVPDDYECEV+
Sbjct: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGGSAQKVETAEKAS-VTE 168
           ENDA VPKHIPLHRPGPLPEEFYKTLEAI GGS+QKVETAEKAS VTE
Sbjct: 121 ENDASVPKHIPLHRPGPLPEEFYKTLEAIGGGSSQKVETAEKASQVTE 168

[Close]
BLAST of Lag0020068 vs. NCBI nr
Match: XP_022968495.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita maxima])

HSP 1 Score: 314.3 bits (804), Expect = 6.3e-82
Identity = 158/170 (92.94%), Postives = 163/170 (95.88%), Query Frame = 0 

Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPWGVPDDYECEVV 120
           TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDEL LI KMIEWDPWGVPDDYECEV+
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIS--GGSAQKVETAEKAS-VTE 168
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAIS  G SAQKVETA+KAS VTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISSGGSSAQKVETADKASQVTE 170

[Close]
BLAST of Lag0020068 vs. ExPASy Swiss-Prot
Match: Q9FLX7 (Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g52840 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 4.5e-75
Identity = 134/160 (83.75%), Postives = 145/160 (90.62%), Query Frame = 0 

Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLA+VKQTTGIVGLDVVPNAR VLI LY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNARAVLIDLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPWGVPDDYECEVV 120
           TR RL VC+EEEDWE IEKRLGCGQVEELIEEA+DELTLIGKMIEWDPWGVPDDYECEV+
Sbjct: 61  TRQRLNVCKEEEDWEMIEKRLGCGQVEELIEEARDELTLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGGSAQKVETA 161
           ENDAP+PKH+P HRPGPLPE+FYKTLE +   S  ++  A
Sbjct: 121 ENDAPIPKHVPQHRPGPLPEQFYKTLEGLIAESKTEIPAA 160

[Close]
BLAST of Lag0020068 vs. ExPASy Swiss-Prot
Match: Q559Z4 (Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Dictyostelium discoideum OX=44689 GN=ndufa5 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 6.6e-18
Identity = 46/107 (42.99%), Postives = 71/107 (66.36%), Query Frame = 0 

Query: 14  VKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K++TGI GL V PNAR +L GLYN+ LK+++ +P   GYRK+ E  T+ RL V + E D
Sbjct: 6   IKKSTGITGLKVEPNARHILAGLYNQLLKKLEILPPTAGYRKSAELETKFRLGVIENETD 65

Query: 74  WEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPWGVPDDYECEVV 121
             +IE ++  GQ+EELI +A+++L ++  + E   W +PD  +  VV
Sbjct: 66  IVKIENKIYAGQIEELIVQAKNDLKVVDLVHESRAWELPDKNKPPVV 112

[Close]
BLAST of Lag0020068 vs. ExPASy Swiss-Prot
Match: P23935 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Bos taurus OX=9913 GN=NDUFA5 PE=1 SV=3)

HSP 1 Score: 82.0 bits (201), Expect = 6.9e-15
Identity = 41/96 (42.71%), Postives = 62/96 (64.58%), Query Frame = 0 

Query: 14  VKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K+TTG+VGL V     E L  LY K L  +  +P++  YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCETPHERLKILYTKILDVLGHIPKNAAYRKYTEQITNEKLSMVKAEPD 64

Query: 74  WEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPW 110
            +++E+RL  GQ+EE+I +A++EL+L  KMI+W PW
Sbjct: 65  VKKLEERLQGGQIEEVILQAENELSLARKMIQWKPW 100

[Close]
BLAST of Lag0020068 vs. ExPASy Swiss-Prot
Match: Q9CPP6 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Mus musculus OX=10090 GN=Ndufa5 PE=1 SV=3)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 40/96 (41.67%), Postives = 59/96 (61.46%), Query Frame = 0 

Query: 14  VKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K+TTG+VGL V     E L  LY KTL  ++  P+   YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCDTPHERLTILYTKTLDILKHFPKHAAYRKYTEQITNEKLDMVKAEPD 64

Query: 74  WEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPW 110
            +++E  L  G+VEE+I +A+ EL+L  KM++W PW
Sbjct: 65  VKKLEALLQGGEVEEVILQAEKELSLARKMLKWKPW 100

[Close]
BLAST of Lag0020068 vs. ExPASy Swiss-Prot
Match: Q4R5J1 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Macaca fascicularis OX=9541 GN=NDUFA5 PE=3 SV=3)

HSP 1 Score: 76.3 bits (186), Expect = 3.8e-13
Identity = 39/96 (40.62%), Postives = 59/96 (61.46%), Query Frame = 0 

Query: 14  VKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K+TTG+VGL V     E L  LY K L  +  +P++  YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCSTPHERLSILYTKILDVLAEIPKNAAYRKYTEQITNEKLAMVKAEPD 64

Query: 74  WEQIEKRLGCGQVEELIEEAQDELTLIGKMIEWDPW 110
            +++E +L  GQ+EE+I +A+ EL+L  KM +W PW
Sbjct: 65  VKKLEDQLQGGQLEEVILQAEHELSLARKMRDWKPW 100

The following BLAST results are available for this feature:
BLAST of Lag0020068 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022945797.19.7e-8394.08probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond... [more]
XP_004140748.11.7e-8293.45probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond... [more]
KAG6574208.12.8e-8293.49putative NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond... [more]
XP_022141236.13.7e-8292.86probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond... [more]
XP_022968495.16.3e-8292.94probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond... [more]
BLAST of Lag0020068 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FLX74.5e-7583.75Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond... [more]
Q559Z46.6e-1842.99Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Dictyos... [more]
P239356.9e-1542.71NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Bos taurus OX=99... [more]
Q9CPP62.2e-1341.67NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Mus musculus OX=... [more]
Q4R5J13.8e-1340.63NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Macaca fascicula... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006806NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5PFAMPF04716ETC_C1_NDUFA5coord: 22..88
e-value: 5.3E-28
score: 96.6
IPR006806NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5PANTHERPTHR12653NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNITcoord: 1..163
NoneNo IPR availablePANTHERPTHR12653:SF1BNAA02G10640D PROTEINcoord: 1..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020068.1Lag0020068.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022904 respiratory electron transport chain
cellular_component GO:0005747 mitochondrial respiratory chain complex I