Lag0020021 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0020021
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr5: 47435976 .. 47437517 (+)
RNA-Seq Expression	Lag0020021
Synteny	Lag0020021

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAGTTCATGGTGGAGTCCACCACTGAAATGCTAAACAAGTGGAGCAGCCTCGTAAACTCCGCCCAACCGGAAATCGACGTGGAGAGAGAAATCACGGCCACCGCCGGCGACATTATCGCTCAGGCCAGCTTCGGAATTTCTAACGGCGGCGGCCGGGACGCCTTCAACAGTCTCAGAGCTCTTCAATTCACTCTCTTCCAAAACAGCACTTACGTCGGAGTTCCTTTCAGCGGCCTCTTTCTCTGCCCGGCCCGAACCCTAGCCGCCAAGCGACTCGGCGCCGAAATCGATCGCCTCTTTTTATCGGTCATATCGGAAAGGAAACAGTCGGAGGAAAATTCTTCTTCCTCCGATTTGCTCAGCCGGTTGATCGAGAGTCAGAGGAACGGCAGCGGCGGCGGCAAGATGAGGCTGACGACGAGAGAACTGGTGGACGAGTGCAAGACTTTCTTCTTTGGCGGCCACGAGACGACGGCGCTGGCGATCAGTTGGACGCTGCTGCTGCTGGCGACGCGGCCGGAGTGGCAGAACATTTTACGGCAGGAGATTAAAGAAGTCATCGGAGATAAAGAGATTGATTTCGCCATGCTTTCTCGACTCAAAAAGGTTAGGGTTTTTTCCTTTTCATCTATGATTTTAAATTTCATATATGGAATTATGGATTTTAATACATCTAATAAATTCTTAAACTTTTAAGTGTTATCAATATTTTTTAAATTTTCGTCGGCATAGTCGATAGATAAAACATCAATTACCAAGTGGATGAGTTCAATCTTCTCACTCCACAATTATATTCTTTTTTAAAAATAGATTAAAAGTTTGACAACCTTAATTTTATAGCCCTAAGTCTATGCATGTAATTATTTTTTTAAGTTTAGCCATTTCTAATTTGAAGTTAATTAAACCAAATTATATAACAATGATATTTCATTTATTGTCAAAGTAAGCATAGTTCAACTCTTCTAAAAAAAAATGACATAGTTCAACAGTAATACAATCTATTTTCAGCATATAAACCCTAAAACTCCCATTTTACAACACTTTTTATAATGATAACTACGTGCTAATGACCACAAGGTGTGTTTGATAAAATGTGTGCAGATGGGATGGGTGTTCAATGAGGTACTTAGGCTATATCCATCAGCCCCAAACATACAAAGACAAGCAAAAGGAGACATCAAAGTCGGCGACGTGACGATCCCAAAAGGGACCAACATATGGATAGACGTTGTATCGATGCATCACGACCCGACCCTTTGGGGTGAGGACGTTAACGAGTTCAAGCCCGAGCGGTTCGAGAAGGACAGCATTCATGGCGGGTGCAACCACAAGATGGGATATTTGCCATTTGGTTTTGGAGGGAGAATGTGCATTGGTAGAAACTTGTCCTCTATGGAGTATAAGATTGTGCTAACCCTAATTCTCTCTAGGTTTTCACTCTCTCTTTCTCCATCCTATTCTCATTCTCCTGCTATTTTGCTCTCTTTGAGACCTGCCCATGGCCTCCCTCTCATTGTAAAGCCCCTCCAACCTTAA

mRNA sequence

ATGGCAAAGTTCATGGTGGAGTCCACCACTGAAATGCTAAACAAGTGGAGCAGCCTCGTAAACTCCGCCCAACCGGAAATCGACGTGGAGAGAGAAATCACGGCCACCGCCGGCGACATTATCGCTCAGGCCAGCTTCGGAATTTCTAACGGCGGCGGCCGGGACGCCTTCAACAGTCTCAGAGCTCTTCAATTCACTCTCTTCCAAAACAGCACTTACGTCGGAGTTCCTTTCAGCGGCCTCTTTCTCTGCCCGGCCCGAACCCTAGCCGCCAAGCGACTCGGCGCCGAAATCGATCGCCTCTTTTTATCGGTCATATCGGAAAGGAAACAGTCGGAGGAAAATTCTTCTTCCTCCGATTTGCTCAGCCGGTTGATCGAGAGTCAGAGGAACGGCAGCGGCGGCGGCAAGATGAGGCTGACGACGAGAGAACTGGTGGACGAGTGCAAGACTTTCTTCTTTGGCGGCCACGAGACGACGGCGCTGGCGATCAGTTGGACGCTGCTGCTGCTGGCGACGCGGCCGGAGTGGCAGAACATTTTACGGCAGGAGATTAAAGAAGTCATCGGAGATAAAGAGATTGATTTCGCCATGCTTTCTCGACTCAAAAAGATGGGATGGGTGTTCAATGAGGTACTTAGGCTATATCCATCAGCCCCAAACATACAAAGACAAGCAAAAGGAGACATCAAAGTCGGCGACGTGACGATCCCAAAAGGGACCAACATATGGATAGACGTTGTATCGATGCATCACGACCCGACCCTTTGGGGTGAGGACGTTAACGAGTTCAAGCCCGAGCGGTTCGAGAAGGACAGCATTCATGGCGGGTGCAACCACAAGATGGGATATTTGCCATTTGGTTTTGGAGGGAGAATGTGCATTGGTAGAAACTTGTCCTCTATGGAGTATAAGATTGTGCTAACCCTAATTCTCTCTAGGTTTTCACTCTCTCTTTCTCCATCCTATTCTCATTCTCCTGCTATTTTGCTCTCTTTGAGACCTGCCCATGGCCTCCCTCTCATTGTAAAGCCCCTCCAACCTTAA

Coding sequence (CDS)

ATGGCAAAGTTCATGGTGGAGTCCACCACTGAAATGCTAAACAAGTGGAGCAGCCTCGTAAACTCCGCCCAACCGGAAATCGACGTGGAGAGAGAAATCACGGCCACCGCCGGCGACATTATCGCTCAGGCCAGCTTCGGAATTTCTAACGGCGGCGGCCGGGACGCCTTCAACAGTCTCAGAGCTCTTCAATTCACTCTCTTCCAAAACAGCACTTACGTCGGAGTTCCTTTCAGCGGCCTCTTTCTCTGCCCGGCCCGAACCCTAGCCGCCAAGCGACTCGGCGCCGAAATCGATCGCCTCTTTTTATCGGTCATATCGGAAAGGAAACAGTCGGAGGAAAATTCTTCTTCCTCCGATTTGCTCAGCCGGTTGATCGAGAGTCAGAGGAACGGCAGCGGCGGCGGCAAGATGAGGCTGACGACGAGAGAACTGGTGGACGAGTGCAAGACTTTCTTCTTTGGCGGCCACGAGACGACGGCGCTGGCGATCAGTTGGACGCTGCTGCTGCTGGCGACGCGGCCGGAGTGGCAGAACATTTTACGGCAGGAGATTAAAGAAGTCATCGGAGATAAAGAGATTGATTTCGCCATGCTTTCTCGACTCAAAAAGATGGGATGGGTGTTCAATGAGGTACTTAGGCTATATCCATCAGCCCCAAACATACAAAGACAAGCAAAAGGAGACATCAAAGTCGGCGACGTGACGATCCCAAAAGGGACCAACATATGGATAGACGTTGTATCGATGCATCACGACCCGACCCTTTGGGGTGAGGACGTTAACGAGTTCAAGCCCGAGCGGTTCGAGAAGGACAGCATTCATGGCGGGTGCAACCACAAGATGGGATATTTGCCATTTGGTTTTGGAGGGAGAATGTGCATTGGTAGAAACTTGTCCTCTATGGAGTATAAGATTGTGCTAACCCTAATTCTCTCTAGGTTTTCACTCTCTCTTTCTCCATCCTATTCTCATTCTCCTGCTATTTTGCTCTCTTTGAGACCTGCCCATGGCCTCCCTCTCATTGTAAAGCCCCTCCAACCTTAA

Protein sequence

MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP

Homology

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BLAST of Lag0020021 vs. NCBI nr
Match: XP_022141037.1 (cytokinin hydroxylase [Momordica charantia])

HSP 1 Score: 586.3 bits (1510), Expect = 1.8e-163
Identity = 293/350 (83.71%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSL 60
           M + MV STT+ML++W+  ++S  PE+DVEREITATAGDIIA+ASFGI +  GRDAFN L
Sbjct: 186 MTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKL 245

Query: 61  RALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSD 120
           R LQFTLF+NS+YVGVPFS +FL P RTL AKRLG+EIDRLFLS+ISERKQS + +S+SD
Sbjct: 246 RDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPD-ASASD 305

Query: 121 LLSRLIESQRNGS--GGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQ 180
           LLSRLIESQRN S    GK  LTTRELVDECKTFFFGGHETTALAISWT+LLLATRPEWQ
Sbjct: 306 LLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQ 365

Query: 181 NILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIP 240
            ILR+EIKEVIGD+EIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD TIP
Sbjct: 366 TILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIP 425

Query: 241 KGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRN 300
           KGTNIWID+V+MHHDP+LWG+DVNEFKPERFEKDSIHGGC HKMGYLPFGFGGRMCIGRN
Sbjct: 426 KGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRN 485

Query: 301 LSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           LSSMEYKIVLTLI+SRFSLS+SPSYSHSPAILLSLRPAHGLPLI+KPL P
Sbjct: 486 LSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534

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BLAST of Lag0020021 vs. NCBI nr
Match: XP_022968014.1 (cytochrome P450 714C2-like [Cucurbita maxima])

HSP 1 Score: 582.8 bits (1501), Expect = 2.0e-162
Identity = 295/348 (84.77%), Postives = 317/348 (91.09%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSL 60
           M KFMVESTT+MLN+WSSLVNSA  EIDVEREITATAGDIIA+ASFGI NGGGRDAF+ L
Sbjct: 178 MTKFMVESTTKMLNEWSSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYL 237

Query: 61  RALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSD 120
           RALQFTLFQNSTYVGVPFS LF CP RTLAAKRLGA IDRLFLS++SERK S+  +SSSD
Sbjct: 238 RALQFTLFQNSTYVGVPFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQ--NSSSD 297

Query: 121 LLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNI 180
           LLSRLIE Q++    GKM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+I
Sbjct: 298 LLSRLIEGQKSTGDVGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDI 357

Query: 181 LRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKG 240
           LR+EIKEVIGDKEIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI+VG +TIPKG
Sbjct: 358 LREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKG 417

Query: 241 TNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLS 300
           TNIWID+V+MH DP LWG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG++LS
Sbjct: 418 TNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLS 477

Query: 301 SMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           SMEYKIVLTLILSRFSLSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 478 SMEYKIVLTLILSRFSLSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523

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BLAST of Lag0020021 vs. NCBI nr
Match: XP_022945852.1 (cytochrome P450 714C2-like [Cucurbita moschata])

HSP 1 Score: 581.3 bits (1497), Expect = 5.7e-162
Identity = 293/348 (84.20%), Postives = 316/348 (90.80%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSL 60
           M KFMVESTT+MLNKWSSLVNSA  EIDVEREITATAGDIIA+ASFGI N GGRDAF+ L
Sbjct: 178 MTKFMVESTTKMLNKWSSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYL 237

Query: 61  RALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSD 120
           RALQFTLFQNSTYVGVPFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++  SSSD
Sbjct: 238 RALQFTLFQNSTYVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSD 297

Query: 121 LLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNI 180
           LLSRLIE +++   GGKM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+I
Sbjct: 298 LLSRLIEGEKSTGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDI 357

Query: 181 LRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKG 240
           LR+EIKEVIGDKEIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKG
Sbjct: 358 LREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKG 417

Query: 241 TNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLS 300
           TNIWID+V+MH DP LWG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLS
Sbjct: 418 TNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLS 477

Query: 301 SMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           SMEYKIVLTLILSRFSLSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 478 SMEYKIVLTLILSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523

[Close]

BLAST of Lag0020021 vs. NCBI nr
Match: KAG6574181.1 (Cytochrome P450 714C2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 580.9 bits (1496), Expect = 7.5e-162
Identity = 293/348 (84.20%), Postives = 316/348 (90.80%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSL 60
           M KFMVESTT+MLNKWSSLVNSA  EIDVEREITATAGDIIA+ASFGI N GGRDAF+ L
Sbjct: 178 MTKFMVESTTKMLNKWSSLVNSAGGEIDVEREITATAGDIIAKASFGICNDGGRDAFDYL 237

Query: 61  RALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSD 120
           RALQFTLFQNSTYVGVPFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++  SSSD
Sbjct: 238 RALQFTLFQNSTYVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSD 297

Query: 121 LLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNI 180
           LLSRLIE +++   GGKM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+I
Sbjct: 298 LLSRLIEGEKSTGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDI 357

Query: 181 LRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKG 240
           LR+EIKEVIGDKEIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKG
Sbjct: 358 LREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKG 417

Query: 241 TNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLS 300
           TNIWID+V+MH DP LWG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLS
Sbjct: 418 TNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLS 477

Query: 301 SMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           SMEYKIVLTLILSRFSLSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 478 SMEYKIVLTLILSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523

[Close]

BLAST of Lag0020021 vs. NCBI nr
Match: KAG7013237.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 580.9 bits (1496), Expect = 7.5e-162
Identity = 293/348 (84.20%), Postives = 316/348 (90.80%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSL 60
           M KFMVESTT+MLNKWSSLVNSA  EIDVEREITATAGDIIA+ASFGI N GGRDAF+ L
Sbjct: 145 MTKFMVESTTKMLNKWSSLVNSAGGEIDVEREITATAGDIIAKASFGICNDGGRDAFDYL 204

Query: 61  RALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSD 120
           RALQFTLFQNSTYVGVPFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++  SSSD
Sbjct: 205 RALQFTLFQNSTYVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSD 264

Query: 121 LLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNI 180
           LLSRLIE +++   GGKM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+I
Sbjct: 265 LLSRLIEGEKSTGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDI 324

Query: 181 LRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKG 240
           LR+EIKEVIGDKEIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKG
Sbjct: 325 LREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKG 384

Query: 241 TNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLS 300
           TNIWID+V+MH DP LWG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLS
Sbjct: 385 TNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLS 444

Query: 301 SMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           SMEYKIVLTLILSRFSLSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 445 SMEYKIVLTLILSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 490

[Close]

BLAST of Lag0020021 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.3e-63
Identity = 138/346 (39.88%), Postives = 199/346 (57.51%), Query Frame = 0


Query: 2   AKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLR 61
           AK MVE T  M  +   L      E+++  E+     DII++  FG S   G++ F+ L 
Sbjct: 173 AKHMVECTKMMAER---LRKEVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLT 232

Query: 62  ALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDL 121
            LQ    Q + ++  P S  FL        K L  E++RL + +I  RK S E   SS  
Sbjct: 233 VLQRLCAQATRHLCFPGS-RFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSY 292

Query: 122 LSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNIL 181
              L+    N     K  L  + ++DECKTFFF GHETT+L ++WTL+LLA  P WQ+ +
Sbjct: 293 GDDLLGLLLNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNV 352

Query: 182 RQEIKEVIGDKEI-DFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKG 241
           R E+++V G   +     LS L  +  V NE LRLYP A  + R A  DIK+GD+ IPKG
Sbjct: 353 RDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKG 412

Query: 242 TNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLS 301
            +IWI V+++HH   LWGED NEF PERF   S      H   ++PF  G R CIG+  +
Sbjct: 413 LSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQTFA 472

Query: 302 SMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 347
            ME KI+L +++S+FS ++S +Y H+P ++L+++P +G+ L++KPL
Sbjct: 473 MMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

[Close]

BLAST of Lag0020021 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.3e-63
Identity = 134/350 (38.29%), Postives = 197/350 (56.29%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLV--NSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFN 60
           M   M+E+   MLN W + V       EI V+  +   + D+I++A FG S   G++ F 
Sbjct: 179 MVNLMMEAAMSMLNSWKNEVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFI 238

Query: 61  SLRALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSS 120
            +R LQ  + + S  +GVP S  +L          L + I  L L++  + +     S +
Sbjct: 239 KIRQLQKAMAKQSMLIGVPGS-RYLPTRSNRGIWNLDSSIRTLILNISKKYEHDSSTSVN 298

Query: 121 SDLLSRLIESQRNGSGGGKMRLTTRE--LVDECKTFFFGGHETTALAISWTLLLLATRPE 180
            DLL  +I+    GS  G     T E  +VD CK  +F GHETT+   +W L+LLA+  E
Sbjct: 299 KDLLHSIIQ----GSKDGPFASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHE 358

Query: 181 WQNILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVT 240
           WQ+  R E  ++   + +DF +L +LKK+  V  E LRLYP A  + R+A  D+K+G + 
Sbjct: 359 WQSRARVESLDICQGRPLDFDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGID 418

Query: 241 IPKGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIG 300
           IPKGTNIWI +   H DP++WG   ++F P+RF  + I G C     Y+PFG G R C G
Sbjct: 419 IPKGTNIWIPIAMAHRDPSVWGPSADKFDPDRF-ANGIAGACKPPHMYMPFGVGVRTCAG 478

Query: 301 RNLSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 347
           +NL+ +E K+VL+L+LS+F   LSP+Y H PA  L++ P  G+PLI + L
Sbjct: 479 QNLAMVELKVVLSLLLSKFEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

[Close]

BLAST of Lag0020021 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.1e-62
Identity = 134/352 (38.07%), Postives = 202/352 (57.39%), Query Frame = 0


Query: 2   AKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLR 61
           A+ MVE T++++ +    V     E+++  E+     DII++  FG S   G++ FN L 
Sbjct: 172 ARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLT 231

Query: 62  ALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS-- 121
            LQ    Q + ++  P S  FL        K L  E++RL + +I  R+   E   SS  
Sbjct: 232 VLQRRCAQATRHLCFPGS-RFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTH 291

Query: 122 --DLLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEW 181
             DLL  L+                + ++DECKTFFF GHETTAL ++WT +LLA  P W
Sbjct: 292 GDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTW 351

Query: 182 QNILRQEIKEVIGDKEI-DFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVT 241
           Q  +R+E++EV G   +     LS+L  +  V NE LRLYP A  + R A  D+K+GD+T
Sbjct: 352 QEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLT 411

Query: 242 IPKGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMG--YLPFGFGGRMC 301
           IPKG +IWI V+++HH   LWG+D N+F PERF      GG     G  ++PF  G R C
Sbjct: 412 IPKGLSIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNC 471

Query: 302 IGRNLSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 347
           IG+  + ME KI+L  ++S+F+ ++S +Y H+P ++L+++P +G+ +I+KPL
Sbjct: 472 IGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of Lag0020021 vs. ExPASy Swiss-Prot
Match: Q93Z79 (Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 124/352 (35.23%), Postives = 194/352 (55.11%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAF 60
           M   +VES   ML+KW  ++        +I V+ ++ A + D+I++A FG S   G++ F
Sbjct: 179 MVGLVVESAMPMLSKWEEMMKREGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIF 238

Query: 61  NSLRALQFTLFQNSTYVGV-PFSGLFLCPARTLAAK--RLGAEIDRLFLSVISERKQSEE 120
           + LR LQ  +  N+    +  F+ +     +    K   L   I+ L    + ER++   
Sbjct: 239 SKLRCLQKAITHNNILFSLNGFTDVVFGTKKHGNGKIDELERHIESLIWETVKERERECV 298

Query: 121 NSSSSDLLSRLIESQRNGSGGG---KMRLTTRELVDECKTFFFGGHETTALAISWTLLLL 180
                DL+  ++E  R+   G    K +     +VD CK+ +F GHET+A+A+SW L+LL
Sbjct: 299 GDHKKDLMQLILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLL 358

Query: 181 ATRPEWQNILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIK 240
           A  P WQ  +R E+     +   D   +S LK +  V  E LRLYP A  + R+A  D K
Sbjct: 359 ALNPSWQTRIRDEVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTK 418

Query: 241 VGDVTIPKGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGG 300
           +G++ +PKG  IW  + ++H DP +WG D NEF PERF  + +   C H   ++PFG G 
Sbjct: 419 LGNLVVPKGVCIWTLIPTLHRDPEIWGADANEFNPERF-SEGVSKACKHPQSFVPFGLGT 478

Query: 301 RMCIGRNLSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 344
           R+C+G+N   ME K++++LI+SRFS +LSP+Y HSP   + + P HG+ + V
Sbjct: 479 RLCLGKNFGMMELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHGVVIRV 529

[Close]

BLAST of Lag0020021 vs. ExPASy Swiss-Prot
Match: Q6NKZ8 (Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.3e-57
Identity = 127/356 (35.67%), Postives = 197/356 (55.34%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAF 60
           M   MVES   MLNKW  +V        +I V+ ++   + D+IA+A FG S   G+  F
Sbjct: 175 MVGLMVESAMPMLNKWEEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIF 234

Query: 61  NSLRALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLG-AEIDRLFLSV-------ISER 120
           + +R L   + + S  V   F+G          +K+ G  +ID L + +       + ER
Sbjct: 235 SMIRDLLTAITKRS--VLFRFNGF---TDMVFGSKKHGDVDIDALEMELESSIWETVKER 294

Query: 121 KQSEENSSSSDLLSRLIESQRNGSGGGKMRLTT--RELVDECKTFFFGGHETTALAISWT 180
           +   +++   DL+  ++E       G     +   R +VD CK+ +F GH++TA+++SW 
Sbjct: 295 EIECKDTHKKDLMQLILEGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWC 354

Query: 181 LLLLATRPEWQNILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAK 240
           L+LLA  P WQ  +R EI     +   D   +  LK +  V  E +RLYP AP + R+A 
Sbjct: 355 LMLLALNPSWQVKIRDEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREAS 414

Query: 241 GDIKVGDVTIPKGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPF 300
            DI++GD+ +PKG  IW  + ++H DP +WG D N+FKPERF  + I   C +   Y+PF
Sbjct: 415 KDIRLGDLVVPKGVCIWTLIPALHRDPEIWGPDANDFKPERF-SEGISKACKYPQSYIPF 474

Query: 301 GFGGRMCIGRNLSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 344
           G G R C+G+N   ME K++++LI+S+FS +LSP+Y HSP+  L + P HG+ + V
Sbjct: 475 GLGPRTCVGKNFGMMEVKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVIRV 524

[Close]

BLAST of Lag0020021 vs. ExPASy TrEMBL
Match: A0A6J1CIQ8 (cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 8.6e-164
Identity = 293/350 (83.71%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSL 60
           M + MV STT+ML++W+  ++S  PE+DVEREITATAGDIIA+ASFGI +  GRDAFN L
Sbjct: 186 MTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKL 245

Query: 61  RALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSD 120
           R LQFTLF+NS+YVGVPFS +FL P RTL AKRLG+EIDRLFLS+ISERKQS + +S+SD
Sbjct: 246 RDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPD-ASASD 305

Query: 121 LLSRLIESQRNGS--GGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQ 180
           LLSRLIESQRN S    GK  LTTRELVDECKTFFFGGHETTALAISWT+LLLATRPEWQ
Sbjct: 306 LLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQ 365

Query: 181 NILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIP 240
            ILR+EIKEVIGD+EIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD TIP
Sbjct: 366 TILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIP 425

Query: 241 KGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRN 300
           KGTNIWID+V+MHHDP+LWG+DVNEFKPERFEKDSIHGGC HKMGYLPFGFGGRMCIGRN
Sbjct: 426 KGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRN 485

Query: 301 LSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           LSSMEYKIVLTLI+SRFSLS+SPSYSHSPAILLSLRPAHGLPLI+KPL P
Sbjct: 486 LSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534

[Close]

BLAST of Lag0020021 vs. ExPASy TrEMBL
Match: A0A6J1HYD5 (cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 9.6e-163
Identity = 295/348 (84.77%), Postives = 317/348 (91.09%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSL 60
           M KFMVESTT+MLN+WSSLVNSA  EIDVEREITATAGDIIA+ASFGI NGGGRDAF+ L
Sbjct: 178 MTKFMVESTTKMLNEWSSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYL 237

Query: 61  RALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSD 120
           RALQFTLFQNSTYVGVPFS LF CP RTLAAKRLGA IDRLFLS++SERK S+  +SSSD
Sbjct: 238 RALQFTLFQNSTYVGVPFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQ--NSSSD 297

Query: 121 LLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNI 180
           LLSRLIE Q++    GKM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+I
Sbjct: 298 LLSRLIEGQKSTGDVGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDI 357

Query: 181 LRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKG 240
           LR+EIKEVIGDKEIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI+VG +TIPKG
Sbjct: 358 LREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKG 417

Query: 241 TNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLS 300
           TNIWID+V+MH DP LWG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG++LS
Sbjct: 418 TNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLS 477

Query: 301 SMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           SMEYKIVLTLILSRFSLSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 478 SMEYKIVLTLILSRFSLSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523

[Close]

BLAST of Lag0020021 vs. ExPASy TrEMBL
Match: A0A6J1G224 (cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.8e-162
Identity = 293/348 (84.20%), Postives = 316/348 (90.80%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSL 60
           M KFMVESTT+MLNKWSSLVNSA  EIDVEREITATAGDIIA+ASFGI N GGRDAF+ L
Sbjct: 178 MTKFMVESTTKMLNKWSSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYL 237

Query: 61  RALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSD 120
           RALQFTLFQNSTYVGVPFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++  SSSD
Sbjct: 238 RALQFTLFQNSTYVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSD 297

Query: 121 LLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNI 180
           LLSRLIE +++   GGKM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+I
Sbjct: 298 LLSRLIEGEKSTGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDI 357

Query: 181 LRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKG 240
           LR+EIKEVIGDKEIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKG
Sbjct: 358 LREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKG 417

Query: 241 TNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLS 300
           TNIWID+V+MH DP LWG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLS
Sbjct: 418 TNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLS 477

Query: 301 SMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           SMEYKIVLTLILSRFSLSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 478 SMEYKIVLTLILSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523

[Close]

BLAST of Lag0020021 vs. ExPASy TrEMBL
Match: A0A5D3DI76 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00640 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.8e-154
Identity = 278/349 (79.66%), Postives = 308/349 (88.25%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISN-GGGRDAFNS 60
           M KFMVESTT+ML +W SLV S Q EI+VER+ITATAG+IIA+ASFGISN GGG +A N+
Sbjct: 121 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 180

Query: 61  LRALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS 120
           LRALQ TLF+NS+YVGVPFS LFL PARTLAAKRLGA+IDRLFLS+IS RK S   S  S
Sbjct: 181 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 240

Query: 121 DLLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQN 180
           DLLSRLI S+  G       L+ RE+VDECKTFFFGGHETTALAISWTLLLLAT P+WQ 
Sbjct: 241 DLLSRLIGSEYTGGEA----LSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQR 300

Query: 181 ILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPK 240
           ILR+EIKEV+GDK+IDF+MLS LKKMGWV+NEVLRLYPSAPNIQRQAKGDI +GD+ IPK
Sbjct: 301 ILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPK 360

Query: 241 GTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNL 300
           GTNIWIDVV+MHHDPTLWG+DVN+F P RF+ D+IHGGCNHKMGYLPFGFGGRMCIGRNL
Sbjct: 361 GTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNL 420

Query: 301 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           SSMEYKIVLTLILS FSLSLSPSYSHSPA LLSLRPAHG+PLI+ PLQP
Sbjct: 421 SSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465

[Close]

BLAST of Lag0020021 vs. ExPASy TrEMBL
Match: A0A5A7SWW2 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001260 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 4.0e-153
Identity = 277/349 (79.37%), Postives = 307/349 (87.97%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISN-GGGRDAFNS 60
           M KFMVESTT+ML +W SLV S Q EI+VER+ITATAG+IIA+ASFGISN GGG +A N+
Sbjct: 121 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 180

Query: 61  LRALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS 120
           LRALQ TLF+NS+YVGVPFS LFL PARTLAAKRLGA+IDRLFLS+IS RK S   S  S
Sbjct: 181 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 240

Query: 121 DLLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQN 180
           DLLSRLI S+  G       L+ RE+VDECKTFFFGGHETTALAISWTLLLLAT P+WQ 
Sbjct: 241 DLLSRLIGSEYTGGEA----LSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQR 300

Query: 181 ILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPK 240
           ILR+EIKEV+GDK+IDF+MLS LKKMGWV+NEVLRLYPSAPNIQRQAKGDI +GD+ IPK
Sbjct: 301 ILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPK 360

Query: 241 GTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNL 300
           GTNIWIDVV+MHHDPTLWG+DVN+F P RF+ D+IHGGC HKMGYLPFGFGGRMCIGRNL
Sbjct: 361 GTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCIGRNL 420

Query: 301 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 349
           SSMEYKIVLTLILS FSLSLSPSYSHSPA LLSLRPAHG+PLI+ PLQP
Sbjct: 421 SSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465

[Close]

BLAST of Lag0020021 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 416.8 bits (1070), Expect = 1.7e-116
Identity = 206/348 (59.20%), Postives = 261/348 (75.00%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSL 60
           M   MVES + ML++W   +NS  PE D+E EI  TAG+IIA+ SFG++   G     +L
Sbjct: 180 MTNMMVESVSNMLDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNL 239

Query: 61  RALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQS--EENSSS 120
           RA+QF LF ++ YVGVPFS + L   +T+ AK LG EID L LS I++RK S  E +   
Sbjct: 240 RAVQFALFNSNRYVGVPFSNI-LSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQG 299

Query: 121 SDLLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQ 180
            DLL  L+++ + G+       T +ELVDECKTFFF GHETTALA++WT +LLA  PEWQ
Sbjct: 300 HDLLGMLLKADQKGN------FTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQ 359

Query: 181 NILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIP 240
           + +R+EI+EVIGD +I++  L+ LKKM WV NEVLRLYP APN QRQA+ DI+V    IP
Sbjct: 360 DTIREEIREVIGDSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIP 419

Query: 241 KGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRN 300
            GTNIWIDVV+MHHD  LWG+DVNEFKPERF+  ++HGGC +KMGY+PFGFGGRMCIGRN
Sbjct: 420 NGTNIWIDVVAMHHDVELWGDDVNEFKPERFD-GNLHGGCKNKMGYMPFGFGGRMCIGRN 479

Query: 301 LSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 347
           L++MEYKIVL+L+LSRF +S+SP Y HSP  +LSLRP +GLPLI++PL
Sbjct: 480 LTTMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

[Close]

BLAST of Lag0020021 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 242.7 bits (618), Expect = 4.5e-64
Identity = 138/346 (39.88%), Postives = 199/346 (57.51%), Query Frame = 0


Query: 2   AKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLR 61
           AK MVE T  M  +   L      E+++  E+     DII++  FG S   G++ F+ L 
Sbjct: 173 AKHMVECTKMMAER---LRKEVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLT 232

Query: 62  ALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDL 121
            LQ    Q + ++  P S  FL        K L  E++RL + +I  RK S E   SS  
Sbjct: 233 VLQRLCAQATRHLCFPGS-RFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSY 292

Query: 122 LSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNIL 181
              L+    N     K  L  + ++DECKTFFF GHETT+L ++WTL+LLA  P WQ+ +
Sbjct: 293 GDDLLGLLLNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNV 352

Query: 182 RQEIKEVIGDKEI-DFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKG 241
           R E+++V G   +     LS L  +  V NE LRLYP A  + R A  DIK+GD+ IPKG
Sbjct: 353 RDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKG 412

Query: 242 TNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLS 301
            +IWI V+++HH   LWGED NEF PERF   S      H   ++PF  G R CIG+  +
Sbjct: 413 LSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQTFA 472

Query: 302 SMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 347
            ME KI+L +++S+FS ++S +Y H+P ++L+++P +G+ L++KPL
Sbjct: 473 MMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

[Close]

BLAST of Lag0020021 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 240.0 bits (611), Expect = 2.9e-63
Identity = 134/352 (38.07%), Postives = 202/352 (57.39%), Query Frame = 0


Query: 2   AKFMVESTTEMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLR 61
           A+ MVE T++++ +    V     E+++  E+     DII++  FG S   G++ FN L 
Sbjct: 172 ARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLT 231

Query: 62  ALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS-- 121
            LQ    Q + ++  P S  FL        K L  E++RL + +I  R+   E   SS  
Sbjct: 232 VLQRRCAQATRHLCFPGS-RFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTH 291

Query: 122 --DLLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEW 181
             DLL  L+                + ++DECKTFFF GHETTAL ++WT +LLA  P W
Sbjct: 292 GDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTW 351

Query: 182 QNILRQEIKEVIGDKEI-DFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVT 241
           Q  +R+E++EV G   +     LS+L  +  V NE LRLYP A  + R A  D+K+GD+T
Sbjct: 352 QEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLT 411

Query: 242 IPKGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMG--YLPFGFGGRMC 301
           IPKG +IWI V+++HH   LWG+D N+F PERF      GG     G  ++PF  G R C
Sbjct: 412 IPKGLSIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNC 471

Query: 302 IGRNLSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 347
           IG+  + ME KI+L  ++S+F+ ++S +Y H+P ++L+++P +G+ +I+KPL
Sbjct: 472 IGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of Lag0020021 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 228.4 bits (581), Expect = 8.8e-60
Identity = 124/352 (35.23%), Postives = 194/352 (55.11%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAF 60
           M   +VES   ML+KW  ++        +I V+ ++ A + D+I++A FG S   G++ F
Sbjct: 179 MVGLVVESAMPMLSKWEEMMKREGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIF 238

Query: 61  NSLRALQFTLFQNSTYVGV-PFSGLFLCPARTLAAK--RLGAEIDRLFLSVISERKQSEE 120
           + LR LQ  +  N+    +  F+ +     +    K   L   I+ L    + ER++   
Sbjct: 239 SKLRCLQKAITHNNILFSLNGFTDVVFGTKKHGNGKIDELERHIESLIWETVKERERECV 298

Query: 121 NSSSSDLLSRLIESQRNGSGGG---KMRLTTRELVDECKTFFFGGHETTALAISWTLLLL 180
                DL+  ++E  R+   G    K +     +VD CK+ +F GHET+A+A+SW L+LL
Sbjct: 299 GDHKKDLMQLILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLL 358

Query: 181 ATRPEWQNILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIK 240
           A  P WQ  +R E+     +   D   +S LK +  V  E LRLYP A  + R+A  D K
Sbjct: 359 ALNPSWQTRIRDEVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTK 418

Query: 241 VGDVTIPKGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGG 300
           +G++ +PKG  IW  + ++H DP +WG D NEF PERF  + +   C H   ++PFG G 
Sbjct: 419 LGNLVVPKGVCIWTLIPTLHRDPEIWGADANEFNPERF-SEGVSKACKHPQSFVPFGLGT 478

Query: 301 RMCIGRNLSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 344
           R+C+G+N   ME K++++LI+SRFS +LSP+Y HSP   + + P HG+ + V
Sbjct: 479 RLCLGKNFGMMELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHGVVIRV 529

[Close]

BLAST of Lag0020021 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 224.2 bits (570), Expect = 1.7e-58
Identity = 127/356 (35.67%), Postives = 197/356 (55.34%), Query Frame = 0


Query: 1   MAKFMVESTTEMLNKWSSLVNSAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAF 60
           M   MVES   MLNKW  +V        +I V+ ++   + D+IA+A FG S   G+  F
Sbjct: 175 MVGLMVESAMPMLNKWEEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIF 234

Query: 61  NSLRALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLG-AEIDRLFLSV-------ISER 120
           + +R L   + + S  V   F+G          +K+ G  +ID L + +       + ER
Sbjct: 235 SMIRDLLTAITKRS--VLFRFNGF---TDMVFGSKKHGDVDIDALEMELESSIWETVKER 294

Query: 121 KQSEENSSSSDLLSRLIESQRNGSGGGKMRLTT--RELVDECKTFFFGGHETTALAISWT 180
           +   +++   DL+  ++E       G     +   R +VD CK+ +F GH++TA+++SW 
Sbjct: 295 EIECKDTHKKDLMQLILEGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWC 354

Query: 181 LLLLATRPEWQNILRQEIKEVIGDKEIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAK 240
           L+LLA  P WQ  +R EI     +   D   +  LK +  V  E +RLYP AP + R+A 
Sbjct: 355 LMLLALNPSWQVKIRDEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREAS 414

Query: 241 GDIKVGDVTIPKGTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPF 300
            DI++GD+ +PKG  IW  + ++H DP +WG D N+FKPERF  + I   C +   Y+PF
Sbjct: 415 KDIRLGDLVVPKGVCIWTLIPALHRDPEIWGPDANDFKPERF-SEGISKACKYPQSYIPF 474

Query: 301 GFGGRMCIGRNLSSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 344
           G G R C+G+N   ME K++++LI+S+FS +LSP+Y HSP+  L + P HG+ + V
Sbjct: 475 GLGPRTCVGKNFGMMEVKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVIRV 524

The following BLAST results are available for this feature:

BLAST of Lag0020021 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141037.1	1.8e-163	83.71	cytokinin hydroxylase [Momordica charantia]	[more]
XP_022968014.1	2.0e-162	84.77	cytochrome P450 714C2-like [Cucurbita maxima]	[more]
XP_022945852.1	5.7e-162	84.20	cytochrome P450 714C2-like [Cucurbita moschata]	[more]
KAG6574181.1	7.5e-162	84.20	Cytochrome P450 714C2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7013237.1	7.5e-162	84.20	Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lag0020021 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZW95	6.3e-63	39.88	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1	[more]
Q2QYH7	8.3e-63	38.29	Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ...	[more]
Q9FF18	4.1e-62	38.07	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1	[more]
Q93Z79	1.2e-58	35.23	Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1	[more]
Q6NKZ8	2.3e-57	35.67	Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1	[more]

BLAST of Lag0020021 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIQ8	8.6e-164	83.71	cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1	[more]
A0A6J1HYD5	9.6e-163	84.77	cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1	[more]
A0A6J1G224	2.8e-162	84.20	cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV...	[more]
A0A5D3DI76	4.8e-154	79.66	Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38...	[more]
A0A5A7SWW2	4.0e-153	79.37	Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold23...	[more]

BLAST of Lag0020021 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52400.1	1.7e-116	59.20	cytochrome P450, family 715, subfamily A, polypeptide 1	[more]
AT1G67110.1	4.5e-64	39.88	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT5G38450.1	2.9e-63	38.07	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]
AT5G24910.1	8.8e-60	35.23	cytochrome P450, family 714, subfamily A, polypeptide 1	[more]
AT5G24900.1	1.7e-58	35.67	cytochrome P450, family 714, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 207..218 score: 41.1 coord: 285..294 score: 57.77 coord: 294..305 score: 36.49 coord: 155..172 score: 39.24
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..322 e-value: 4.0E-73 score: 246.7
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 164..190 score: 35.69 coord: 294..317 score: 33.23 coord: 144..161 score: 28.15 coord: 284..294 score: 51.95
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..345 e-value: 2.7E-88 score: 298.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..345
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 1..342
None	No IPR available	PANTHER	PTHR24282:SF149	CYTOCHROME P450 FAMILY 72 PROTEIN	coord: 1..342
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 287..296

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0020021.1	Lag0020021.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen