Lag0019951 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0019951
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	subtilisin-like protease SBT1.3
Location	chr5: 46909412 .. 46910395 (+)
RNA-Seq Expression	Lag0019951
Synteny	Lag0019951

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGATTCTCTTGTGAAATGGCTCTGTTTGCTTCTGTTTCTTCACTGTTTGCTTCTTTCAAGAGCTCAGCTTTTGAAAACTTATGTCGTTCAAATGGACAGGTCTGCAATGCCGGCCTCATTCTCGGATCATTTCCAGTGGTACTCCACAGTTCTAAGCAATGTGGTGGTTAGCCCTGAGAGACAAGGTAATGGGGGAGGTGAAGAGAGGATCATTTACAGTTACCAGAGTGTTTTCCATGGAGTTGCAGCTCGGTTGAGTGAAGAAGAGACTGAGAGGCTCGAGGAAGAAGATGGGGTGTTGGCCATTTTTCCGGAGACTAAGTATGAGCTTCATACAACGAGAAGCCCCAAGTTTCTTGGGCTTGAACCGGCTGACAGCAACAGCGCCTGGTCTCAGCAGATCGCAGACCATGACGTGATCGTTGGAGTTTTGGATACTGGGATTTGGCCGGAGAGTGAGAGCTTCAACGACGCTGGAATGTCGCCGGTGCCGCCGCATTGGAAAGGGGAATGTGAAACAGGGCGAGGCTTTACGAAACACAATTGTAATAGAAAGATCGTCGGCGCCAGAGTGTTCTACCATGGCTATGAAGCTGCGACTGGGAAATTTAACGAACAGTTGGAGTACAAATCGCCGAGGGATCAAGATGGGCATGGAACTCACACGGCGGCTACCGTCGCCGGCTCTCCGGTGGCCGGCGCGAGCCTCCTTGGTTATGCTTATGGAACGGCCAGAGGAATGGCGCCCGGTGCTAGAATTGCCGCCTACAAAGTCTGTTGGGTCGGCGGCTGCTTTAGCTCCGACATTTTGTCGGCTGTGGATAGAGCTGTGGCCGACGGAGTAAATGTTCTGTCCATCTCTTTGGGAGGTGGGGTCTCTTCTTACTATCGCGATAGCCTTTCAGTTGCAGCATTTGGGCAATGGAGATGGGCGTGTTTGTCTCCTGTTCGGCCGGAAATGGAGGGCCGGACCCTGTAA

mRNA sequence

ATGGCTGATTCTCTTGTGAAATGGCTCTGTTTGCTTCTGTTTCTTCACTGTTTGCTTCTTTCAAGAGCTCAGCTTTTGAAAACTTATGTCGTTCAAATGGACAGGTCTGCAATGCCGGCCTCATTCTCGGATCATTTCCAGTGGTACTCCACAGTTCTAAGCAATGTGGTGGTTAGCCCTGAGAGACAAGGTAATGGGGGAGGTGAAGAGAGGATCATTTACAGTTACCAGAGTGTTTTCCATGGAGTTGCAGCTCGGTTGAGTGAAGAAGAGACTGAGAGGCTCGAGGAAGAAGATGGGGTGTTGGCCATTTTTCCGGAGACTAAGTATGAGCTTCATACAACGAGAAGCCCCAAGTTTCTTGGGCTTGAACCGGCTGACAGCAACAGCGCCTGGTCTCAGCAGATCGCAGACCATGACGTGATCGTTGGAGTTTTGGATACTGGGATTTGGCCGGAGAGTGAGAGCTTCAACGACGCTGGAATGTCGCCGGTGCCGCCGCATTGGAAAGGGGAATGTGAAACAGGGCGAGGCTTTACGAAACACAATTGTAATAGAAAGATCGTCGGCGCCAGAGTGTTCTACCATGGCTATGAAGCTGCGACTGGGAAATTTAACGAACAGTTGGAGTACAAATCGCCGAGGGATCAAGATGGGCATGGAACTCACACGGCGGCTACCGTCGCCGGCTCTCCGGTGGCCGGCGCGAGCCTCCTTGGTTATGCTTATGGAACGGCCAGAGGAATGGCGCCCGGTGCTAGAATTGCCGCCTACAAAGTCTGTTGGGTCGGCGGCTGCTTTAGCTCCGACATTTTGTCGGCTGTGGATAGAGCTGTGGCCGACGGAGTAAATGTTCTGTCCATCTCTTTGGGAGGTGGGGTCTCTTCTTACTATCGCGATAGCCTTTCAGTTGCAGCATTTGGGCAATGGAGATGGGCGTGTTTGTCTCCTGTTCGGCCGGAAATGGAGGGCCGGACCCTGTAA

Coding sequence (CDS)

ATGGCTGATTCTCTTGTGAAATGGCTCTGTTTGCTTCTGTTTCTTCACTGTTTGCTTCTTTCAAGAGCTCAGCTTTTGAAAACTTATGTCGTTCAAATGGACAGGTCTGCAATGCCGGCCTCATTCTCGGATCATTTCCAGTGGTACTCCACAGTTCTAAGCAATGTGGTGGTTAGCCCTGAGAGACAAGGTAATGGGGGAGGTGAAGAGAGGATCATTTACAGTTACCAGAGTGTTTTCCATGGAGTTGCAGCTCGGTTGAGTGAAGAAGAGACTGAGAGGCTCGAGGAAGAAGATGGGGTGTTGGCCATTTTTCCGGAGACTAAGTATGAGCTTCATACAACGAGAAGCCCCAAGTTTCTTGGGCTTGAACCGGCTGACAGCAACAGCGCCTGGTCTCAGCAGATCGCAGACCATGACGTGATCGTTGGAGTTTTGGATACTGGGATTTGGCCGGAGAGTGAGAGCTTCAACGACGCTGGAATGTCGCCGGTGCCGCCGCATTGGAAAGGGGAATGTGAAACAGGGCGAGGCTTTACGAAACACAATTGTAATAGAAAGATCGTCGGCGCCAGAGTGTTCTACCATGGCTATGAAGCTGCGACTGGGAAATTTAACGAACAGTTGGAGTACAAATCGCCGAGGGATCAAGATGGGCATGGAACTCACACGGCGGCTACCGTCGCCGGCTCTCCGGTGGCCGGCGCGAGCCTCCTTGGTTATGCTTATGGAACGGCCAGAGGAATGGCGCCCGGTGCTAGAATTGCCGCCTACAAAGTCTGTTGGGTCGGCGGCTGCTTTAGCTCCGACATTTTGTCGGCTGTGGATAGAGCTGTGGCCGACGGAGTAAATGTTCTGTCCATCTCTTTGGGAGGTGGGGTCTCTTCTTACTATCGCGATAGCCTTTCAGTTGCAGCATTTGGGCAATGGAGATGGGCGTGTTTGTCTCCTGTTCGGCCGGAAATGGAGGGCCGGACCCTGTAA

Protein sequence

MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQGNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGQWRWACLSPVRPEMEGRTL

Homology

[Close]

BLAST of Lag0019951 vs. NCBI nr
Match: XP_022922929.1 (subtilisin-like protease SBT1.3 [Cucurbita moschata])

HSP 1 Score: 569.7 bits (1467), Expect = 1.6e-158
Identity = 279/308 (90.58%), Postives = 293/308 (95.13%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   CLLLS AQ +KTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ERQGNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKF 120
           ER+GNGGGE+RIIYSYQ+VFHGVAARL+EEE ERLEEE+GVLAIFPETKYELHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           K NCNRKIVGAR+FYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFG 309
           SLSVAAFG
Sbjct: 301 SLSVAAFG 308

[Close]

BLAST of Lag0019951 vs. NCBI nr
Match: XP_023552616.1 (subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 569.3 bits (1466), Expect = 2.1e-158
Identity = 279/308 (90.58%), Postives = 292/308 (94.81%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   CLLLS AQ +KTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ERQGNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKF 120
           ER+GNGGGE+RIIYSYQ+VFHGVAARLSEEE ERLEEEDGVLAIFPETKYELHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR F+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180

Query: 181 KHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           K NCNRKIVGAR+FYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFG 309
           SLSVAAFG
Sbjct: 301 SLSVAAFG 308

[Close]

BLAST of Lag0019951 vs. NCBI nr
Match: KAG6576764.1 (Subtilisin-like protease 1.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 568.2 bits (1463), Expect = 4.7e-158
Identity = 277/308 (89.94%), Postives = 292/308 (94.81%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           M  + +KWLCLLL   CLLLS AQ +KTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MGHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ERQGNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKF 120
           ER+GNGGGE+RIIYSYQ+VFHGVAARLSE+E ERLEEEDGVLAIFPETKYELHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEDEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           K NCNRKIVGAR+FYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFG 309
           SLSVAAFG
Sbjct: 301 SLSVAAFG 308

[Close]

BLAST of Lag0019951 vs. NCBI nr
Match: XP_022984431.1 (subtilisin-like protease SBT1.3 [Cucurbita maxima])

HSP 1 Score: 566.2 bits (1458), Expect = 1.8e-157
Identity = 278/308 (90.26%), Postives = 291/308 (94.48%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   C LLS AQ LKTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ERQGNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKF 120
           ER+GNGGGE+RIIYSYQ+VFHGVAARLSEEE ERLEEEDGVLAIFPETKY+LHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           K NCNRKIVGAR+FYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSP A A+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFG 309
           SLSVAAFG
Sbjct: 301 SLSVAAFG 308

[Close]

BLAST of Lag0019951 vs. NCBI nr
Match: KAG7014803.1 (Subtilisin-like protease SBT1.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 565.8 bits (1457), Expect = 2.3e-157
Identity = 276/308 (89.61%), Postives = 292/308 (94.81%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   CLL S AQ +KTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCLLHSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ERQGNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKF 120
           ER+GNGGGE+RIIYSYQ+VFHGVAARLSE+E ERLEEEDGVLAIFPETKYELHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEDEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           K NCNRKIVGA++FYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGAKMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFG 309
           SLSVAAFG
Sbjct: 301 SLSVAAFG 308

[Close]

BLAST of Lag0019951 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 6.9e-120
Identity = 207/297 (69.70%), Postives = 245/297 (82.49%), Query Frame = 0


Query: 12  LLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQGNGGGEER 71
           L+FL     ++    KTYV+ MD+SAMP  +++H QWYS+ + N V   + Q   G   R
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKI-NSVTQHKSQEEEGNNNR 79

Query: 72  IIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSA 131
           I+Y+YQ+ FHG+AA+L++EE ERLEEEDGV+A+ PET+YELHTTRSP FLGLE  +S   
Sbjct: 80  ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERV 139

Query: 132 WSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHNCNRKIVGA 191
           W++++ DHDV+VGVLDTGIWPESESFND GMSPVP  W+G CETG+ F K NCNRKIVGA
Sbjct: 140 WAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGA 199

Query: 192 RVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAP 251
           RVFY GYEAATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA 
Sbjct: 200 RVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQ 259

Query: 252 GARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFG 309
            AR+AAYKVCWVGGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FG
Sbjct: 260 KARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFG 315

[Close]

BLAST of Lag0019951 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.2e-85
Identity = 156/304 (51.32%), Postives = 210/304 (69.08%), Query Frame = 0


Query: 4   SLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQ 63
           S   +  LL    C + S +    TY+V M +S MP+SF  H  WY + L ++  S E  
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE-- 66

Query: 64  GNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGL 123
                   ++Y+Y++  HG + RL++EE + L  + GV+++ PE +YELHTTR+P FLGL
Sbjct: 67  --------LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 126

Query: 124 EPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHN 183
           +   ++  + +  +  DV+VGVLDTG+WPES+S++D G  P+P  WKG CE G  FT   
Sbjct: 127 DEHTAD-LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 186

Query: 184 CNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAY 243
           CNRK++GAR F  GYE+  G  +E  E +SPRD DGHGTHT++T AGS V GASLLGYA 
Sbjct: 187 CNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 246

Query: 244 GTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLS 303
           GTARGMAP AR+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD ++
Sbjct: 247 GTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 299

Query: 304 VAAF 308
           + AF
Sbjct: 307 IGAF 299

[Close]

BLAST of Lag0019951 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 7.8e-79
Identity = 141/281 (50.18%), Postives = 189/281 (67.26%), Query Frame = 0


Query: 28  TYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQGNGGGEERIIYSYQSVFHGVAARL 87
           TY+V +D  A P+ F  HF WY++ L+++  SP           II++Y +VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------PSIIHTYDTVFHGFSARL 86

Query: 88  SEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           + ++  +L +   V+++ PE    LHTTRSP+FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHNCNRKIVGARVFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   + F +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 QLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCF 267
             E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFG 309
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFG
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFG 298

[Close]

BLAST of Lag0019951 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 4.9e-73
Identity = 146/307 (47.56%), Postives = 197/307 (64.17%), Query Frame = 0


Query: 9   LCLLLFLHCLLLSRA--QLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQGNG 68
           + LLLFL    +S A  Q  KT++ ++D  +MP+ F  H+ WYST  +            
Sbjct: 6   IVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE----------- 65

Query: 69  GGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPA 128
             E RI++ Y +VFHG +A ++ +E + L     VLA+F + + ELHTTRSP+FLGL+  
Sbjct: 66  --ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ-- 125

Query: 129 DSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHNCNR 188
           +    WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+  NCNR
Sbjct: 126 NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNR 185

Query: 189 KIVGARVFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGT 248
           KI+GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     AS+ GYA G 
Sbjct: 186 KIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGV 245

Query: 249 ARGMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDS 308
           A+G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D 
Sbjct: 246 AKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDP 297

[Close]

BLAST of Lag0019951 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 6.4e-73
Identity = 145/305 (47.54%), Postives = 195/305 (63.93%), Query Frame = 0


Query: 11  LLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQGNGGGEE 70
           LLL       S +  L++Y+V + RS  P+ FS H  W+ ++L ++  SP+         
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ-------PA 74

Query: 71  RIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNS 130
            ++YSY    HG +ARLS  +T  L     V+++ P+   E+HTT +P FLG   + ++ 
Sbjct: 75  TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF--SQNSG 134

Query: 131 AWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHNCNRKIVG 190
            WS      DVIVGVLDTGIWPE  SF+D+G+ P+P  WKGECE G  F   +CNRK++G
Sbjct: 135 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 194

Query: 191 ARVFYHGY--EAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARG 250
           AR FY GY  +    K +   E +SPRD +GHGTHTA+T AGS VA ASL  YA GTA G
Sbjct: 195 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 254

Query: 251 MAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLG--GGVSSYYRDSLSVAA 310
           MA  ARIAAYK+CW GGC+ SDIL+A+D+AVADGV+V+S+S+G  G    Y+ DS+++ A
Sbjct: 255 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 310

Query: 311 FGQWR 312
           FG  R
Sbjct: 315 FGATR 310

[Close]

BLAST of Lag0019951 vs. ExPASy TrEMBL
Match: A0A6J1E4V2 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111430759 PE=3 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 7.9e-159
Identity = 279/308 (90.58%), Postives = 293/308 (95.13%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   CLLLS AQ +KTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ERQGNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKF 120
           ER+GNGGGE+RIIYSYQ+VFHGVAARL+EEE ERLEEE+GVLAIFPETKYELHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           K NCNRKIVGAR+FYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFG 309
           SLSVAAFG
Sbjct: 301 SLSVAAFG 308

[Close]

BLAST of Lag0019951 vs. ExPASy TrEMBL
Match: A0A6J1J576 (subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111482733 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 8.7e-158
Identity = 278/308 (90.26%), Postives = 291/308 (94.48%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MA + +KWLCLLL   C LLS AQ LKTYVVQMDRSAMP SFSDH QWYSTVL++VVV+P
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ERQGNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKF 120
           ER+GNGGGE+RIIYSYQ+VFHGVAARLSEEE ERLEEEDGVLAIFPETKY+LHTTRSP+F
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           K NCNRKIVGAR+FYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSP A A+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFG 309
           SLSVAAFG
Sbjct: 301 SLSVAAFG 308

[Close]

BLAST of Lag0019951 vs. ExPASy TrEMBL
Match: A0A5A7SUB4 (Subtilisin-like protease SBT1.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00040 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.4e-157
Identity = 280/309 (90.61%), Postives = 294/309 (95.15%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MADS +K LCLLLFL  LLLS A  LKTYVVQMDRSAMPASF++HF+WYS VLSNVVV  
Sbjct: 1   MADSPIKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  ERQGN-GGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPK 120
           ER+GN GGGEERIIYSYQ+VFHGVAARLSEEE E+LEEEDGV+AIFPE KYELHTTRSP+
Sbjct: 61  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 120

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPES+SF+DAGMSPVP HWKGECETGRGF
Sbjct: 121 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 180

Query: 181 TKHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TK NCNRKIVGARVFYHGY+AATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 241 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300

Query: 301 DSLSVAAFG 309
           DSLSVAAFG
Sbjct: 301 DSLSVAAFG 309

[Close]

BLAST of Lag0019951 vs. ExPASy TrEMBL
Match: A0A1S3AY00 (subtilisin-like protease SBT1.3 OS=Cucumis melo OX=3656 GN=LOC103484020 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.2e-156
Identity = 279/309 (90.29%), Postives = 293/309 (94.82%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSP 60
           MAD  +K LCLLLFL  LLLS A  LKTYVVQMDRSAMPASF++HF+WYS VLSNVVV  
Sbjct: 1   MADFPLKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  ERQGN-GGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPK 120
           ER+GN GGGEERIIYSYQ+VFHGVAARLSEEE E+LEEEDGV+AIFPE KYELHTTRSP+
Sbjct: 61  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 120

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPES+SF+DAGMSPVP HWKGECETGRGF
Sbjct: 121 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 180

Query: 181 TKHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TK NCNRKIVGARVFYHGY+AATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 241 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300

Query: 301 DSLSVAAFG 309
           DSLSVAAFG
Sbjct: 301 DSLSVAAFG 309

[Close]

BLAST of Lag0019951 vs. ExPASy TrEMBL
Match: A0A6J1FZU3 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111449392 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.4e-155
Identity = 277/311 (89.07%), Postives = 291/311 (93.57%), Query Frame = 0


Query: 1   MADSLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVV-S 60
           MA + ++WLC  LF HCL  S AQ  KTYVVQMDRSAMP SFSDH +WYSTV+S+VVV +
Sbjct: 1   MAHTPLQWLCFPLFFHCLFFSSAQFFKTYVVQMDRSAMPDSFSDHLEWYSTVVSSVVVDN 60

Query: 61  PER--QGNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRS 120
           PER  QGNGGGE+RIIYSYQ+VFHGVAARLSEEE ERLEEE GVLA+FPE KYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGR 180
           PKFLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVP +WKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKHNCNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGAS 240
           GFTK NCNRKIVGARVFYHGYEAATGKFN+QLEYKSPRDQDGHGTHTAATVAGSPVAGA+
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFG 309
           YRDSLSVAAFG
Sbjct: 301 YRDSLSVAAFG 311

[Close]

BLAST of Lag0019951 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 431.8 bits (1109), Expect = 4.9e-121
Identity = 207/297 (69.70%), Postives = 245/297 (82.49%), Query Frame = 0


Query: 12  LLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQGNGGGEER 71
           L+FL     ++    KTYV+ MD+SAMP  +++H QWYS+ + N V   + Q   G   R
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKI-NSVTQHKSQEEEGNNNR 79

Query: 72  IIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSA 131
           I+Y+YQ+ FHG+AA+L++EE ERLEEEDGV+A+ PET+YELHTTRSP FLGLE  +S   
Sbjct: 80  ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERV 139

Query: 132 WSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHNCNRKIVGA 191
           W++++ DHDV+VGVLDTGIWPESESFND GMSPVP  W+G CETG+ F K NCNRKIVGA
Sbjct: 140 WAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGA 199

Query: 192 RVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAP 251
           RVFY GYEAATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA 
Sbjct: 200 RVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQ 259

Query: 252 GARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFG 309
            AR+AAYKVCWVGGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FG
Sbjct: 260 KARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFG 315

[Close]

BLAST of Lag0019951 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 316.2 bits (809), Expect = 3.0e-86
Identity = 156/304 (51.32%), Postives = 210/304 (69.08%), Query Frame = 0


Query: 4   SLVKWLCLLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQ 63
           S   +  LL    C + S +    TY+V M +S MP+SF  H  WY + L ++  S E  
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE-- 66

Query: 64  GNGGGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGL 123
                   ++Y+Y++  HG + RL++EE + L  + GV+++ PE +YELHTTR+P FLGL
Sbjct: 67  --------LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 126

Query: 124 EPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHN 183
           +   ++  + +  +  DV+VGVLDTG+WPES+S++D G  P+P  WKG CE G  FT   
Sbjct: 127 DEHTAD-LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 186

Query: 184 CNRKIVGARVFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAY 243
           CNRK++GAR F  GYE+  G  +E  E +SPRD DGHGTHT++T AGS V GASLLGYA 
Sbjct: 187 CNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 246

Query: 244 GTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLS 303
           GTARGMAP AR+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD ++
Sbjct: 247 GTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 299

Query: 304 VAAF 308
           + AF
Sbjct: 307 IGAF 299

[Close]

BLAST of Lag0019951 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 295.4 bits (755), Expect = 5.5e-80
Identity = 141/281 (50.18%), Postives = 189/281 (67.26%), Query Frame = 0


Query: 28  TYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQGNGGGEERIIYSYQSVFHGVAARL 87
           TY+V +D  A P+ F  HF WY++ L+++  SP           II++Y +VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------PSIIHTYDTVFHGFSARL 86

Query: 88  SEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           + ++  +L +   V+++ PE    LHTTRSP+FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHNCNRKIVGARVFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   + F +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 QLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCF 267
             E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFG 309
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFG
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFG 298

[Close]

BLAST of Lag0019951 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 276.2 bits (705), Expect = 3.5e-74
Identity = 146/307 (47.56%), Postives = 197/307 (64.17%), Query Frame = 0


Query: 9   LCLLLFLHCLLLSRA--QLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQGNG 68
           + LLLFL    +S A  Q  KT++ ++D  +MP+ F  H+ WYST  +            
Sbjct: 6   IVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE----------- 65

Query: 69  GGEERIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPA 128
             E RI++ Y +VFHG +A ++ +E + L     VLA+F + + ELHTTRSP+FLGL+  
Sbjct: 66  --ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ-- 125

Query: 129 DSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHNCNR 188
           +    WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+  NCNR
Sbjct: 126 NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNR 185

Query: 189 KIVGARVFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGT 248
           KI+GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     AS+ GYA G 
Sbjct: 186 KIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGV 245

Query: 249 ARGMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDS 308
           A+G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D 
Sbjct: 246 AKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDP 297

[Close]

BLAST of Lag0019951 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 275.8 bits (704), Expect = 4.5e-74
Identity = 145/305 (47.54%), Postives = 195/305 (63.93%), Query Frame = 0


Query: 11  LLLFLHCLLLSRAQLLKTYVVQMDRSAMPASFSDHFQWYSTVLSNVVVSPERQGNGGGEE 70
           LLL       S +  L++Y+V + RS  P+ FS H  W+ ++L ++  SP+         
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ-------PA 74

Query: 71  RIIYSYQSVFHGVAARLSEEETERLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNS 130
            ++YSY    HG +ARLS  +T  L     V+++ P+   E+HTT +P FLG   + ++ 
Sbjct: 75  TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF--SQNSG 134

Query: 131 AWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKHNCNRKIVG 190
            WS      DVIVGVLDTGIWPE  SF+D+G+ P+P  WKGECE G  F   +CNRK++G
Sbjct: 135 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 194

Query: 191 ARVFYHGY--EAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARG 250
           AR FY GY  +    K +   E +SPRD +GHGTHTA+T AGS VA ASL  YA GTA G
Sbjct: 195 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 254

Query: 251 MAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLG--GGVSSYYRDSLSVAA 310
           MA  ARIAAYK+CW GGC+ SDIL+A+D+AVADGV+V+S+S+G  G    Y+ DS+++ A
Sbjct: 255 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 310

Query: 311 FGQWR 312
           FG  R
Sbjct: 315 FGATR 310

The following BLAST results are available for this feature:

BLAST of Lag0019951 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922929.1	1.6e-158	90.58	subtilisin-like protease SBT1.3 [Cucurbita moschata]	[more]
XP_023552616.1	2.1e-158	90.58	subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo]	[more]
KAG6576764.1	4.7e-158	89.94	Subtilisin-like protease 1.3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022984431.1	1.8e-157	90.26	subtilisin-like protease SBT1.3 [Cucurbita maxima]	[more]
KAG7014803.1	2.3e-157	89.61	Subtilisin-like protease SBT1.3, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]

BLAST of Lag0019951 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLI4	6.9e-120	69.70	Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S...	[more]
O65351	4.2e-85	51.32	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
Q9LUM3	7.8e-79	50.18	Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S...	[more]
O49607	4.9e-73	47.56	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S...	[more]
Q9LVJ1	6.4e-73	47.54	Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S...	[more]

BLAST of Lag0019951 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E4V2	7.9e-159	90.58	subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111430759 PE...	[more]
A0A6J1J576	8.7e-158	90.26	subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111482733 PE=3...	[more]
A0A5A7SUB4	7.4e-157	90.61	Subtilisin-like protease SBT1.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3AY00	6.2e-156	90.29	subtilisin-like protease SBT1.3 OS=Cucumis melo OX=3656 GN=LOC103484020 PE=3 SV=...	[more]
A0A6J1FZU3	1.4e-155	89.07	subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111449392 PE...	[more]

BLAST of Lag0019951 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51750.1	4.9e-121	69.70	subtilase 1.3	[more]
AT5G67360.1	3.0e-86	51.32	Subtilase family protein	[more]
AT3G14240.1	5.5e-80	50.18	Subtilase family protein	[more]
AT4G34980.1	3.5e-74	47.56	subtilisin-like serine protease 2	[more]
AT3G14067.1	4.5e-74	47.54	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PRINTS	PR00723	SUBTILISIN	coord: 216..229 score: 50.79 coord: 138..157 score: 31.6
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 20..113 e-value: 5.9E-19 score: 70.3
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 139..296 e-value: 8.3E-19 score: 67.9
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 140..307 e-value: 5.2E-69 score: 235.2
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 112..306
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 28..113 e-value: 2.4E-16 score: 60.2
None	No IPR available	PANTHER	PTHR10795:SF675	SUBTILISIN-LIKE PROTEASE SBT1.7	coord: 17..308
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 109..327 score: 15.318243
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 17..308
IPR034197	Cucumisin-like catalytic domain	CDD	cd04852	Peptidases_S8_3	coord: 110..308 e-value: 4.95868E-104 score: 305.291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0019951.1	Lag0019951.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity