Lag0019924 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019924
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionMitochondrial intermediate peptidase, putative
Locationchr5: 46746271 .. 46749459 (+)
RNA-Seq ExpressionLag0019924
SyntenyLag0019924
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGCTCGGTTCCATATATTCTGCAGACAGTTTTTTCAACTCAATTTTTCCGTCAGTTTTCATTAATGCACATCCTTAACTTCCCCCCTTTCTGAAACATCAAATTTACTCCCCTAAACTCCATATTTCTGTTTCAGGGTGACTTGGGATATTTATACCTGGATTTGTACTCAAGAAAAGGAAAATATCCAGGTTGTGCTCATTTTGCAATTAAGGGAGGCCGCAAGGTTTCTGAAATGGAATACCAACTTCCTGTATGTATCAAATAGAATTTCATGCAACTTTTGATTTCTATAATACTTCCTACTACAGCTGTTTGTCTGTATCTGATTTAACATTTTTGTGATGCTTGAATTCTTTTTTCTGCAAAATTGAATAGACATGGAATCCTCTCATAGCTATTAGTAATCAGCTGTAATCAAGAATAATGTGAAATTCTACTTATGCTTCCTAGGTGAGATAGAAAGTGGATTGATTGTTTGGTGAAGGGGAACTGATATTCCTTATTATTACGATGGTGTATGCCTGGGATGAAAGTATGTAGCTTATGATTCTCGTTTGCTAATATCTGAGAAATATCAGCCTAATTAAATGATCTTGCATGTTCTCTTTCAACTTGTAACCAAAAGGTTGATAATTCGATGCTAATAATATATCTATTCTAGAAAGATCATTAGGAGAAGGTTATTAGAACTTCCTGTATGTATCAAATAGAATACCAACTTCCTGTATGTATCAAATAGAATTTCATGCAACTTTTGATTTCTATAATACTTCCTACTACAGCTGTTTGTCTGTATCTGATTTAACATTTTTGTGATGCTTGAATTCTTTTTTCTGCAAAATTGAATAGACATGGAATCCTCTCATAGCTATTAGTAATCAGCTGTAATCAAGAATAATGTGAAATTCTACTTATGCTTCCTAGGTGAGATAGAAAGTGGATTGATTGTTTGGTGAAGGGGAACTGATATTCCTTATTATTACGATGGTGTATGCCTGGGATGAAAGTATGTAGCTTATGATTCTCGTTTGCTAATATCTGAGAAATATCAGCCTAATTAAATGATCTTGCATGTTCTCTTTCAACTTGTAACCAAAAGGTTGATAATTCGATGCTAATAATATATCTATTCTAGAAAGATCATTAGGAGAAGGTTATTAGAAATGCAAAGCCATAAGACTGATTTAGTCACCTTCCAAATCAAACGTTTGTGTTGGTTATGTGCAGGTTGTAGCTCTCATTTGCAATTTTTCTGGTTCACATGATCGATCAAATGTGAGGCTTAATCATTCAGAAGTAGAAATTCTTTTCCACGAGTTTGGACATGCTCTTCATTCACTGCTTTCCAGAACGGTATGCGCTGATTCTTTATTATTGAACAGACAAGTTTTAAACCCCCTCCCCCTCCGGGTTTCTTTGTTTCTTCTGTTATATATATAACTCATCAGTTCTCGACTACTACAGGAATATCAACATTTTTCAGGTACAAGAGTGGTTCTTGATCTGGCTGAGACACCTTCAAACCTATTTGAGTGAGTAAAAATGTCCCGTTTCTTACTTGCCCTTTGGTTTACGAAGATCATATAAGTTAAGCTATATAATGTTTACTATTGCCTTTCTAATAAAGACATCCATTTATCTTTATCATATATGAGCACTAACTTATATAATTATCCTCTTGTAAATATGGTGATACTGCAATTATTTAACGTGTCAACAGCATGGACAGGACCCTGTTGGCTTAGAATAAGTAGTGCATGTGTGTAGTCTTGTACATATAACCTGACGAAACCCTTCTGTTAGAGTCTTGTTTTATATCAGCGGTTGTTATTGCATAATAATAGCCATCACCAATATTTGATGACTAGGTACTATGCATCAGATTATCGTGTTTTGAGAACGTTTGCTAAGCACTATTCAACTGGTGATATAATTCCTGAAAAGCTTGTAAAGTCAATGCAGGGTGCCAAAAAGATGTTTGCAGCCACAGAACTACAGCGTCAGGTGACAATGTTGCTGGTTAACTGTGAGCATATATTATATTTTGATCTGAACAAACCAATTTTTACTTGCTTCTTTGATTACCAGATTCTTTATGCCTTAATTGATCAAACACTGTTTGGAGAAAGGCTAGCTTCAGAGAAGGATACTAGTTCTGTTGTTGCTGAGCTAAAAAGACAATGTACCAGTTGGAAGCATGTGGATGGCACTCATTGGCAGACCCGATTTTGCCACCTCCTGACTTATGGTGCAGGTTGCAAAAGTCGATCTATTTTTGTGTTTGAATGTGATATATTTTCGCTCTTCAATTTGATATGAGATCTTTAAGCTTTGAGTGACTGATCTGATCTCTTTCCACCAGCCGGATTGAGGAACAAAGAGCCTCGATTTCTTGGTCTCATTATAATTTATAAGAGCTAATTTCTCACGTCTTGCATGATAGTTACTGTAGTTTGGAATTCAAAATAACCAAAATACCCATCTACAATATTTCCTGAACCAACATGAATTGCTTCTTTTCTTTGTAGATTTTACAAAAACTGAAACAGCATGCAATTGAGTTATCCATATATCTTCCTTATTTCTCTTTTTTCATTAGACAATTCATTTCAAGAAATGGAAAATCCAAATTCTAGGCGACTTCAACTTTAGTCATCTTAAATGGAAGATATACCTACGCTATATAATGCATCCTGTCGCCATGTCTAGATTCATTTTGTAATTGATAGCATATGATATCTCTTTCTACATAATTGGAAACAACTTTAGGAGCCGGTTTGGAATGACTTTTCAAGTGCTTATAAAAAGGTTTTTACGTGTTAAAAATTTGTTTTTAAACACTTGATAAGTCATTCCAAACAGGTTCATATTAGTTGATATCTCCTTTTTCATTTCTTTCCGATGTGAGATATTCACACGTTTGTTTTTACAGGTTACTACAGCTACCTGTACGCCAAATGTTTCGCTGCAACGATATGGGAGAAGCTTTGTAAGGATGATCCTCTTTCAAGACACACAGGAACTGCCTTGAGAACAAAGTTTCTGCAACATGGCGGCTCCAAAGAACCAGTTGAGTTATTAACTGATCTCGTGGGAGATGGGATCATAAGGTATTGTGAAGGAGGAGTAGTTCCTGATATAACGAGTCTTTGCAAGGAAATGGAGCTTAGTGGAAACTTGTAG

mRNA sequence

ATGGGTGACTTGGGATATTTATACCTGGATTTGTACTCAAGAAAAGGAAAATATCCAGGTTGTGCTCATTTTGCAATTAAGGGAGGCCGCAAGGTTTCTGAAATGGAATACCAACTTCCTGTTGTAGCTCTCATTTGCAATTTTTCTGGTTCACATGATCGATCAAATGTGAGGCTTAATCATTCAGAAGTAGAAATTCTTTTCCACGAGTTTGGACATGCTCTTCATTCACTGCTTTCCAGAACGGAATATCAACATTTTTCAGGTACAAGAGTGGTTCTTGATCTGGCTGAGACACCTTCAAACCTATTTGAGTACTATGCATCAGATTATCGTGTTTTGAGAACGTTTGCTAAGCACTATTCAACTGGTGATATAATTCCTGAAAAGCTTGTAAAGTCAATGCAGGGTGCCAAAAAGATGTTTGCAGCCACAGAACTACAGCGTCAGATTCTTTATGCCTTAATTGATCAAACACTGTTTGGAGAAAGGCTAGCTTCAGAGAAGGATACTAGTTCTGTTGTTGCTGAGCTAAAAAGACAATGTACCAGTTGGAAGCATGTGGATGGCACTCATTGGCAGACCCGATTTTGCCACCTCCTGACTTATGGTGCAGGTTACTACAGCTACCTGTACGCCAAATGTTTCGCTGCAACGATATGGGAGAAGCTTTGTAAGGATGATCCTCTTTCAAGACACACAGGAACTGCCTTGAGAACAAAGTTTCTGCAACATGGCGGCTCCAAAGAACCAGTTGAGTTATTAACTGATCTCGTGGGAGATGGGATCATAAGGTATTGTGAAGGAGGAGTAGTTCCTGATATAACGAGTCTTTGCAAGGAAATGGAGCTTAGTGGAAACTTGTAG

Coding sequence (CDS)

ATGGGTGACTTGGGATATTTATACCTGGATTTGTACTCAAGAAAAGGAAAATATCCAGGTTGTGCTCATTTTGCAATTAAGGGAGGCCGCAAGGTTTCTGAAATGGAATACCAACTTCCTGTTGTAGCTCTCATTTGCAATTTTTCTGGTTCACATGATCGATCAAATGTGAGGCTTAATCATTCAGAAGTAGAAATTCTTTTCCACGAGTTTGGACATGCTCTTCATTCACTGCTTTCCAGAACGGAATATCAACATTTTTCAGGTACAAGAGTGGTTCTTGATCTGGCTGAGACACCTTCAAACCTATTTGAGTACTATGCATCAGATTATCGTGTTTTGAGAACGTTTGCTAAGCACTATTCAACTGGTGATATAATTCCTGAAAAGCTTGTAAAGTCAATGCAGGGTGCCAAAAAGATGTTTGCAGCCACAGAACTACAGCGTCAGATTCTTTATGCCTTAATTGATCAAACACTGTTTGGAGAAAGGCTAGCTTCAGAGAAGGATACTAGTTCTGTTGTTGCTGAGCTAAAAAGACAATGTACCAGTTGGAAGCATGTGGATGGCACTCATTGGCAGACCCGATTTTGCCACCTCCTGACTTATGGTGCAGGTTACTACAGCTACCTGTACGCCAAATGTTTCGCTGCAACGATATGGGAGAAGCTTTGTAAGGATGATCCTCTTTCAAGACACACAGGAACTGCCTTGAGAACAAAGTTTCTGCAACATGGCGGCTCCAAAGAACCAGTTGAGTTATTAACTGATCTCGTGGGAGATGGGATCATAAGGTATTGTGAAGGAGGAGTAGTTCCTGATATAACGAGTCTTTGCAAGGAAATGGAGCTTAGTGGAAACTTGTAG

Protein sequence

MGDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNHSEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQCTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTKFLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL
Homology
BLAST of Lag0019924 vs. NCBI nr
Match: XP_022941717.1 (mitochondrial intermediate peptidase, mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 266/287 (92.68%), Postives = 279/287 (97.21%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVAL+CNFSGS +RSNVRLNH
Sbjct: 309 GDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNH 368

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 369 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 428

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG++IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERL SE+DTSSVVAELKRQ
Sbjct: 429 STGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQ 488

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
           CTSWKHVDGTHWQTRF HLLTYGAGYYSYLYAKCFAATIWEKLC++DPLSR TGTALRTK
Sbjct: 489 CTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTK 548

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKEPV+LL DLVGDGIIRYC+GGV+PDITSLCKEM++SG L
Sbjct: 549 FLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKL 595

BLAST of Lag0019924 vs. NCBI nr
Match: XP_023518581.1 (mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 266/287 (92.68%), Postives = 279/287 (97.21%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVAL+CNF GS +RSNVRLNH
Sbjct: 432 GDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFYGSRERSNVRLNH 491

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 492 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 551

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG++IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERL SE+DTSSVVAELKRQ
Sbjct: 552 STGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQ 611

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
           CTSWKHVDGTHWQTRF HLLTYGAGYYSYLYAKCFAATIWEKLC++DPLSR TGTALRTK
Sbjct: 612 CTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTK 671

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKEPV+LL DLVGDGIIRYC+GGV+PDITSLCKEM++SGNL
Sbjct: 672 FLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGNL 718

BLAST of Lag0019924 vs. NCBI nr
Match: XP_022941716.1 (mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 266/287 (92.68%), Postives = 279/287 (97.21%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVAL+CNFSGS +RSNVRLNH
Sbjct: 432 GDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNH 491

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 492 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 551

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG++IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERL SE+DTSSVVAELKRQ
Sbjct: 552 STGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQ 611

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
           CTSWKHVDGTHWQTRF HLLTYGAGYYSYLYAKCFAATIWEKLC++DPLSR TGTALRTK
Sbjct: 612 CTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTK 671

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKEPV+LL DLVGDGIIRYC+GGV+PDITSLCKEM++SG L
Sbjct: 672 FLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKL 718

BLAST of Lag0019924 vs. NCBI nr
Match: XP_023518590.1 (mitochondrial intermediate peptidase, mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 266/287 (92.68%), Postives = 279/287 (97.21%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVAL+CNF GS +RSNVRLNH
Sbjct: 309 GDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFYGSRERSNVRLNH 368

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 369 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 428

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG++IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERL SE+DTSSVVAELKRQ
Sbjct: 429 STGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQ 488

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
           CTSWKHVDGTHWQTRF HLLTYGAGYYSYLYAKCFAATIWEKLC++DPLSR TGTALRTK
Sbjct: 489 CTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTK 548

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKEPV+LL DLVGDGIIRYC+GGV+PDITSLCKEM++SGNL
Sbjct: 549 FLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGNL 595

BLAST of Lag0019924 vs. NCBI nr
Match: KAG6600162.1 (Mitochondrial intermediate peptidase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 558.9 bits (1439), Expect = 2.5e-155
Identity = 265/287 (92.33%), Postives = 279/287 (97.21%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVAL+CNFSGS +RSNVRLNH
Sbjct: 390 GDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNH 449

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 450 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 509

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG++IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERL SE+DTSS+VAELKRQ
Sbjct: 510 STGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERDTSSLVAELKRQ 569

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
           CTSWKHVDGTHWQTRF HLLTYGAGYYSYLYAKCFAATIWEKLC++DPLSR TGTALRTK
Sbjct: 570 CTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTK 629

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKEPV+LL DLVGDGIIRYC+GGV+PDITSLCKEM++SG L
Sbjct: 630 FLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKL 676

BLAST of Lag0019924 vs. ExPASy Swiss-Prot
Match: F4KDA5 (Mitochondrial intermediate peptidase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OCT1 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.1e-121
Identity = 205/283 (72.44%), Postives = 237/283 (83.75%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCA FAI+GGRK+SE EYQLPV+AL+CNFS + D S V+LNH
Sbjct: 424 GDLGYLYLDLYSRKGKYPGCASFAIRGGRKISETEYQLPVIALVCNFSRACDSSIVKLNH 483

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE+LFHEFGHALHSLLSRT+YQHFSGTRV LDLAE PSNLFEYYA DYR+L+ FA+HY
Sbjct: 484 SEVEVLFHEFGHALHSLLSRTDYQHFSGTRVALDLAEMPSNLFEYYAWDYRLLKRFARHY 543

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG+ IPEKLV S+QGA+ MFAATE+QRQ+ YALIDQ LFGE+  + +D S +VAELKRQ
Sbjct: 544 STGETIPEKLVNSLQGARNMFAATEMQRQVFYALIDQMLFGEQPETARDVSHLVAELKRQ 603

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
            TSW HV+GTHW  RF HLL YGAGYYSYLYAKCFA+TIW+ +C++DPLS +TGT LR K
Sbjct: 604 HTSWNHVEGTHWYIRFSHLLNYGAGYYSYLYAKCFASTIWQSICEEDPLSLNTGTLLREK 663

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMEL 285
           F +HGG+K+P ELLTDL G  II     G+VP  T L  E+ L
Sbjct: 664 FFKHGGAKDPAELLTDLAGKEIISVHGEGIVPATTYLLNELRL 706

BLAST of Lag0019924 vs. ExPASy Swiss-Prot
Match: Q99797 (Mitochondrial intermediate peptidase OS=Homo sapiens OX=9606 GN=MIPEP PE=1 SV=2)

HSP 1 Score: 223.8 bits (569), Expect = 2.5e-57
Identity = 114/283 (40.28%), Postives = 167/283 (59.01%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           G LGY+Y D + R  K     HF I+GGR   + +YQLPVV L+ N   S   S   L  
Sbjct: 428 GLLGYIYCDFFQRADKPHQDCHFTIRGGRLKEDGDYQLPVVVLMLNLPRSSRSSPTLLTP 487

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           S +E LFHE GHA+HS+L RT YQH +GTR   D AE PS L EY+A+DYRV+  FA+HY
Sbjct: 488 SMMENLFHEMGHAMHSMLGRTRYQHVTGTRCPTDFAEVPSILMEYFANDYRVVNQFARHY 547

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
            TG  +P+ +V  +  +KK+ AA ++Q Q+ YA +DQ   G+       T+ ++ E + +
Sbjct: 548 QTGQPLPKNMVSRLCESKKVCAAADMQLQVFYATLDQIYHGKH-PLRNSTTDILKETQEK 607

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
                +V  T WQ RF HL+ YGA YYSYL ++  A+ +W++    DP +R  G   R +
Sbjct: 608 FYGLPYVPNTAWQLRFSHLVGYGARYYSYLMSRAVASMVWKECFLQDPFNRAAGERYRRE 667

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMEL 285
            L HGG +EP+     L+ +G+++ C   V   +++L  +++L
Sbjct: 668 MLAHGGGREPM-----LMVEGMLQKCP-SVDDFVSALVSDLDL 703

BLAST of Lag0019924 vs. ExPASy Swiss-Prot
Match: Q5RF14 (Mitochondrial intermediate peptidase OS=Pongo abelii OX=9601 GN=MIPEP PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 9.6e-57
Identity = 113/283 (39.93%), Postives = 166/283 (58.66%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           G LGY+Y D + R  K     HF I+GGR   + +YQLPVV L+ N   S   S   L  
Sbjct: 428 GLLGYIYCDFFQRADKPHQDCHFTIRGGRLKEDGDYQLPVVVLMLNLPRSSRSSPTLLTP 487

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
             +E LFHE GHA+HS+L RT YQH +GTR   D AE PS L EY+A+DYRV+  FA+HY
Sbjct: 488 GMMENLFHEMGHAMHSMLGRTRYQHVTGTRCPTDFAEVPSILMEYFANDYRVVNQFARHY 547

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
            TG  +P+ +V  +  +KK+ AA ++Q Q+ YA +DQ   G+       T+ ++ E + +
Sbjct: 548 QTGQPLPKNMVSRLCESKKVCAAADMQLQVFYATLDQIYHGKH-PLRNSTTDILKETQEK 607

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
                +V  T WQ RF HL+ YGA YYSYL ++  A+ +W++    DP +R  G   R +
Sbjct: 608 FYGLPYVPDTAWQLRFSHLVGYGAKYYSYLMSRAVASMVWKECFLQDPFNRAAGERYRRE 667

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMEL 285
            L HGG +EP+     L+ +G+++ C   V   +++L  +++L
Sbjct: 668 MLAHGGGREPM-----LMVEGMLQKCP-SVDDFVSALVSDLDL 703

BLAST of Lag0019924 vs. ExPASy Swiss-Prot
Match: Q01992 (Mitochondrial intermediate peptidase OS=Rattus norvegicus OX=10116 GN=Mipep PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.1e-56
Identity = 109/258 (42.25%), Postives = 153/258 (59.30%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           G LGY+Y D + R  K     HF I+GGR   +  YQLPVV L+ N   +       L  
Sbjct: 425 GLLGYIYCDFFQRANKPQQDCHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTP 484

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
             +E LFHE GHA+HS+L RT YQH +GTR   D AE PS L EY+++DYRV+  FAKHY
Sbjct: 485 GMMENLFHEMGHAMHSMLGRTRYQHVTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHY 544

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
            TG  +P+ +V  +  +KK+ AA E+Q Q+ YA +DQ   G+    +K T+ ++ E + Q
Sbjct: 545 QTGQPLPKAMVSRLCESKKVCAAAEMQLQVFYAALDQIYHGQH-PLKKSTTDILMETQEQ 604

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
                +V  T WQ RF HL+ YGA YYSYL ++  A+ +W++    DP +R  G   R +
Sbjct: 605 FYGLPYVPDTAWQLRFSHLVGYGAKYYSYLMSRAVASMVWKECFLQDPFNRAAGERYRRE 664

Query: 242 FLQHGGSKEPVELLTDLV 260
            L HGG KEP+ ++  ++
Sbjct: 665 MLAHGGGKEPMLMIQGML 681

BLAST of Lag0019924 vs. ExPASy Swiss-Prot
Match: A6H611 (Mitochondrial intermediate peptidase OS=Mus musculus OX=10090 GN=Mipep PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 4.8e-56
Identity = 109/258 (42.25%), Postives = 152/258 (58.91%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           G LGY+Y D + R  K     HF I+GGR   +  YQLPVV L+ N   +       L  
Sbjct: 426 GLLGYIYCDFFQRANKPQQDCHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTP 485

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
             +E LFHE GHA+HS+L RT YQH +GTR   D AE PS L EY+++DYRV+  FAKHY
Sbjct: 486 GMMENLFHEMGHAMHSMLGRTRYQHVTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHY 545

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
            TG  +P+ +V  +  +KK+  A E+Q Q+ YA +DQ   G+    +K T+ ++ E + Q
Sbjct: 546 QTGQPLPKAMVSRLCESKKVCTAAEMQLQVFYAALDQIYHGQH-PLKKSTTDILMETQEQ 605

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
                +V  T WQ RF HL+ YGA YYSYL ++  A+ IW++    DP +R  G   R +
Sbjct: 606 FYGLPYVPDTAWQLRFSHLVGYGAKYYSYLMSRAVASMIWKECFLQDPFNRAAGERYRRE 665

Query: 242 FLQHGGSKEPVELLTDLV 260
            L HGG KEP+ ++  ++
Sbjct: 666 MLAHGGGKEPMLMIQGML 682

BLAST of Lag0019924 vs. ExPASy TrEMBL
Match: A0A6J1FN99 (mitochondrial intermediate peptidase, mitochondrial isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446997 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.5e-156
Identity = 266/287 (92.68%), Postives = 279/287 (97.21%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVAL+CNFSGS +RSNVRLNH
Sbjct: 309 GDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNH 368

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 369 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 428

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG++IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERL SE+DTSSVVAELKRQ
Sbjct: 429 STGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQ 488

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
           CTSWKHVDGTHWQTRF HLLTYGAGYYSYLYAKCFAATIWEKLC++DPLSR TGTALRTK
Sbjct: 489 CTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTK 548

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKEPV+LL DLVGDGIIRYC+GGV+PDITSLCKEM++SG L
Sbjct: 549 FLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKL 595

BLAST of Lag0019924 vs. ExPASy TrEMBL
Match: A0A6J1FLV9 (mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446997 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.5e-156
Identity = 266/287 (92.68%), Postives = 279/287 (97.21%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVAL+CNFSGS +RSNVRLNH
Sbjct: 432 GDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNH 491

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 492 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 551

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG++IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERL SE+DTSSVVAELKRQ
Sbjct: 552 STGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQ 611

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
           CTSWKHVDGTHWQTRF HLLTYGAGYYSYLYAKCFAATIWEKLC++DPLSR TGTALRTK
Sbjct: 612 CTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTK 671

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKEPV+LL DLVGDGIIRYC+GGV+PDITSLCKEM++SG L
Sbjct: 672 FLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKL 718

BLAST of Lag0019924 vs. ExPASy TrEMBL
Match: A0A6J1CH32 (probable mitochondrial intermediate peptidase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011592 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.1e-155
Identity = 264/287 (91.99%), Postives = 279/287 (97.21%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GGRKVSEMEYQLPVVAL+CNFS SH RSNVRLNH
Sbjct: 403 GDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNH 462

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 463 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 522

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STGDIIPEKLVKSMQGAKKMF ATELQRQILYALIDQTLFGERL SE+DTSSVVAELKRQ
Sbjct: 523 STGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQ 582

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
            T+WKHVD THWQTRFCHLLTYGAGYYSYLYAKCF+ATIWEKLC++DPLSR+TGTALRTK
Sbjct: 583 YTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTK 642

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKE V+LLTDLVGDGI+RYC+GGV+PD+TSLCKEM+LSG+L
Sbjct: 643 FLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL 689

BLAST of Lag0019924 vs. ExPASy TrEMBL
Match: A0A6J1I911 (mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471087 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.1e-155
Identity = 264/287 (91.99%), Postives = 278/287 (96.86%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVAL+CNFSGS +RSNVRLNH
Sbjct: 432 GDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNH 491

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 492 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 551

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG++IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERL  E+DTSSVVAELKRQ
Sbjct: 552 STGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTLERDTSSVVAELKRQ 611

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
           CTSWKHVDGTHWQTRF HLLTYGAGYYSYLYAKCFAATIWEKLC++DPLSR TGTALRTK
Sbjct: 612 CTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTK 671

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKEPV+LL DL+GDGIIRYC+GGV+PDITSLCKEM++SG L
Sbjct: 672 FLQHGGSKEPVDLLKDLLGDGIIRYCDGGVIPDITSLCKEMDISGKL 718

BLAST of Lag0019924 vs. ExPASy TrEMBL
Match: A0A6J1I6J0 (mitochondrial intermediate peptidase, mitochondrial isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471087 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.1e-155
Identity = 264/287 (91.99%), Postives = 278/287 (96.86%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVAL+CNFSGS +RSNVRLNH
Sbjct: 309 GDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFSGSRERSNVRLNH 368

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE LFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY
Sbjct: 369 SEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 428

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG++IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERL  E+DTSSVVAELKRQ
Sbjct: 429 STGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTLERDTSSVVAELKRQ 488

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
           CTSWKHVDGTHWQTRF HLLTYGAGYYSYLYAKCFAATIWEKLC++DPLSR TGTALRTK
Sbjct: 489 CTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDPLSRSTGTALRTK 548

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMELSGNL 289
           FLQHGGSKEPV+LL DL+GDGIIRYC+GGV+PDITSLCKEM++SG L
Sbjct: 549 FLQHGGSKEPVDLLKDLLGDGIIRYCDGGVIPDITSLCKEMDISGKL 595

BLAST of Lag0019924 vs. TAIR 10
Match: AT5G51540.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 437.6 bits (1124), Expect = 7.9e-123
Identity = 205/283 (72.44%), Postives = 237/283 (83.75%), Query Frame = 0

Query: 2   GDLGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNH 61
           GDLGYLYLDLYSRKGKYPGCA FAI+GGRK+SE EYQLPV+AL+CNFS + D S V+LNH
Sbjct: 424 GDLGYLYLDLYSRKGKYPGCASFAIRGGRKISETEYQLPVIALVCNFSRACDSSIVKLNH 483

Query: 62  SEVEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHY 121
           SEVE+LFHEFGHALHSLLSRT+YQHFSGTRV LDLAE PSNLFEYYA DYR+L+ FA+HY
Sbjct: 484 SEVEVLFHEFGHALHSLLSRTDYQHFSGTRVALDLAEMPSNLFEYYAWDYRLLKRFARHY 543

Query: 122 STGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAELKRQ 181
           STG+ IPEKLV S+QGA+ MFAATE+QRQ+ YALIDQ LFGE+  + +D S +VAELKRQ
Sbjct: 544 STGETIPEKLVNSLQGARNMFAATEMQRQVFYALIDQMLFGEQPETARDVSHLVAELKRQ 603

Query: 182 CTSWKHVDGTHWQTRFCHLLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTALRTK 241
            TSW HV+GTHW  RF HLL YGAGYYSYLYAKCFA+TIW+ +C++DPLS +TGT LR K
Sbjct: 604 HTSWNHVEGTHWYIRFSHLLNYGAGYYSYLYAKCFASTIWQSICEEDPLSLNTGTLLREK 663

Query: 242 FLQHGGSKEPVELLTDLVGDGIIRYCEGGVVPDITSLCKEMEL 285
           F +HGG+K+P ELLTDL G  II     G+VP  T L  E+ L
Sbjct: 664 FFKHGGAKDPAELLTDLAGKEIISVHGEGIVPATTYLLNELRL 706

BLAST of Lag0019924 vs. TAIR 10
Match: AT1G67690.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 115.9 bits (289), Expect = 5.3e-26
Identity = 80/262 (30.53%), Postives = 132/262 (50.38%), Query Frame = 0

Query: 4   LGYLYLDLYSRKGKYPGCAHFAIKGGRKVSEMEYQLPVVALICNFSGSHDRSNVRLNHSE 63
           LGY YLD+++R+GK       A++     S    Q+PV  LI  F+       V L  S+
Sbjct: 437 LGYFYLDMFTREGKCNHSCVVALQNNALFSNGACQIPVALLIAQFAKDGSGEAVPLGFSD 496

Query: 64  VEILFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYST 123
           V  LFHEFGH +  + +R  +  FSG RV  D  E PS L E +  +   L+  + +   
Sbjct: 497 VVNLFHEFGHVVQHICNRASFARFSGLRVDPDFREIPSQLLENWCYESFTLKLISGYRQ- 556

Query: 124 GDI---IPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVVAEL-K 183
            DI   + +++ K+++  +  F+A +  ++ILY L DQ ++ +    + D   ++  L  
Sbjct: 557 -DITKPLVDEVCKTLKRWRYSFSALKSLQEILYCLFDQIIYSD---DDADLLQLIRSLHP 616

Query: 184 RQCTSWKHVDGTHWQTRFCH-LLTYGAGYYSYLYAKCFAATIWEKLCKDDPLSRHTGTAL 243
           +       V+GT+  + F   ++   A  YS L+++ +AA I+     D   + + G   
Sbjct: 617 KVMIGLPVVEGTNPASCFPRAVIGSEATCYSRLWSEVYAADIFASKFGDGHPNLYAGLQF 676

Query: 244 RTKFLQHGGSKEPVELLTDLVG 261
           R K L  GG KEP+ELLT+ +G
Sbjct: 677 RDKVLAPGGGKEPMELLTNFLG 693

BLAST of Lag0019924 vs. TAIR 10
Match: AT5G65620.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 100.1 bits (248), Expect = 3.0e-21
Identity = 77/271 (28.41%), Postives = 124/271 (45.76%), Query Frame = 0

Query: 4   LGYLYLDLYSRKGKYPGCAHF--AIKGGRKVSE--MEYQLPVVALICNFSGSHDRSNVRL 63
           + Y Y D YSR  +  G A     +   R +++     +LPV  ++CN +         +
Sbjct: 502 IAYFYFDPYSRPSEKRGGAWMDEVVSRSRVMAQKGSSVRLPVAHMVCNQTPPVGDKPSLM 561

Query: 64  NHSEVEILFHEFGHALHSLLSRTEYQHFSGTR-VVLDLAETPSNLFEYYASDYRVLRTFA 123
              EVE +FHEFGHAL  +L++ +    +G R +  D  E PS   E +      L + A
Sbjct: 562 TFREVETVFHEFGHALQHMLTKQDEGLVAGIRNIEWDAVELPSQFMENWCYHRDTLMSIA 621

Query: 124 KHYSTGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVV--- 183
           KHY TG+ +PE++ K +  A+   A +   RQ+ +A +D  L  + +    ++   V   
Sbjct: 622 KHYETGETLPEEVYKKLLAARTFRAGSFSLRQLKFASVDLELHTKYVPGGPESIYDVDQR 681

Query: 184 AELKRQCTSWKHVDGTHWQTRFCHLLT--YGAGYYSYLYAKCFAA---TIWEKLCKDD-P 243
             +K Q       D   +   F H+    Y AGYYSY +A+  +A   + +E    DD  
Sbjct: 682 VSVKTQVIPPLPED--RFLCSFSHIFAGGYAAGYYSYKWAEVLSADAFSAFEDAGLDDIK 741

Query: 244 LSRHTGTALRTKFLQHGGSKEPVELLTDLVG 261
             + TG   R   L  GG K P+++  +  G
Sbjct: 742 AVKETGQRFRNTILALGGGKAPLKVFVEFRG 770

BLAST of Lag0019924 vs. TAIR 10
Match: AT5G10540.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 99.4 bits (246), Expect = 5.1e-21
Identity = 78/269 (29.00%), Postives = 120/269 (44.61%), Query Frame = 0

Query: 6   YLYLDLYSRKG-KYPGCAHFAIKGGRKVSEME---YQLPVVALICNFSGSHDRSNVRLNH 65
           Y Y D YSR   K  G     +    +V   +    +LPV  ++CN +         +  
Sbjct: 416 YFYFDPYSRPSEKRDGAWMDEVFSRSRVMAQKGSSVRLPVAQMVCNQTPPVGDKPSLMTF 475

Query: 66  SEVEILFHEFGHALHSLLSRTEYQHFSGTR-VVLDLAETPSNLFEYYASDYRVLRTFAKH 125
            EVE +FHEFGHAL  +L++ +    +G R +  D  E PS   E +      L + AKH
Sbjct: 476 REVETVFHEFGHALQHMLTKEDEGLVAGIRNIEWDAVELPSQFMENWCYHRDTLMSIAKH 535

Query: 126 YSTGDIIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLASEKDTSSVV---AE 185
           Y TG+ +PE + K +  A+   A +   RQ+ +A +D  L  + +    +T   V     
Sbjct: 536 YQTGETLPENVYKKLLAARTFRAGSLSLRQLKFATVDLELHTKYMPGGAETIYEVDQRVS 595

Query: 186 LKRQCTSWKHVDGTHWQTRFCHLLT--YGAGYYSYLYAKCFAA---TIWEKLCKDD-PLS 245
           +K Q       D   +   F H+    Y AGYYSY +A+  +A   + +E    DD    
Sbjct: 596 IKTQVIPPLPED--RFLCSFSHIFAGGYAAGYYSYKWAEVLSADAFSAFEDAGLDDIKAV 655

Query: 246 RHTGTALRTKFLQHGGSKEPVELLTDLVG 261
           + TG   R   L  GG K P+++  +  G
Sbjct: 656 KETGQRFRNTILALGGGKAPLKVFVEFRG 682

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022941717.11.1e-15592.68mitochondrial intermediate peptidase, mitochondrial isoform X2 [Cucurbita moscha... [more]
XP_023518581.11.1e-15592.68mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita pepo s... [more]
XP_022941716.11.1e-15592.68mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita moscha... [more]
XP_023518590.11.1e-15592.68mitochondrial intermediate peptidase, mitochondrial isoform X2 [Cucurbita pepo s... [more]
KAG6600162.12.5e-15592.33Mitochondrial intermediate peptidase, mitochondrial, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
F4KDA51.1e-12172.44Mitochondrial intermediate peptidase, mitochondrial OS=Arabidopsis thaliana OX=3... [more]
Q997972.5e-5740.28Mitochondrial intermediate peptidase OS=Homo sapiens OX=9606 GN=MIPEP PE=1 SV=2[more]
Q5RF149.6e-5739.93Mitochondrial intermediate peptidase OS=Pongo abelii OX=9601 GN=MIPEP PE=2 SV=1[more]
Q019922.1e-5642.25Mitochondrial intermediate peptidase OS=Rattus norvegicus OX=10116 GN=Mipep PE=1... [more]
A6H6114.8e-5642.25Mitochondrial intermediate peptidase OS=Mus musculus OX=10090 GN=Mipep PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FN995.5e-15692.68mitochondrial intermediate peptidase, mitochondrial isoform X2 OS=Cucurbita mosc... [more]
A0A6J1FLV95.5e-15692.68mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita mosc... [more]
A0A6J1CH322.1e-15591.99probable mitochondrial intermediate peptidase, mitochondrial OS=Momordica charan... [more]
A0A6J1I9116.1e-15591.99mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita maxi... [more]
A0A6J1I6J06.1e-15591.99mitochondrial intermediate peptidase, mitochondrial isoform X2 OS=Cucurbita maxi... [more]
Match NameE-valueIdentityDescription
AT5G51540.17.9e-12372.44Zincin-like metalloproteases family protein [more]
AT1G67690.15.3e-2630.53Zincin-like metalloproteases family protein [more]
AT5G65620.13.0e-2128.41Zincin-like metalloproteases family protein [more]
AT5G10540.15.1e-2129.00Zincin-like metalloproteases family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001567Peptidase M3A/M3B catalytic domainPFAMPF01432Peptidase_M3coord: 4..261
e-value: 3.9E-76
score: 257.2
NoneNo IPR availableGENE3D1.10.1370.40coord: 1..189
e-value: 8.7E-62
score: 211.5
coord: 190..263
e-value: 1.5E-8
score: 36.0
NoneNo IPR availablePANTHERPTHR11804:SF79MITOCHONDRIAL INTERMEDIATE PEPTIDASE, MITOCHONDRIALcoord: 2..284
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 4..260
IPR045090Peptidase M3A/M3BPANTHERPTHR11804PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATEDcoord: 2..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019924.1Lag0019924.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006518 peptide metabolic process
biological_process GO:0006508 proteolysis
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity