Lag0019914 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0019914
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pectinesterase
Location	chr5: 46674583 .. 46676924 (-)
RNA-Seq Expression	Lag0019914
Synteny	Lag0019914

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCACAAAACAAAATTTCAATTTTTTCCATAACCCTTTTGGCAATTCTTTCAAATGTCTCTTCACAAGCAAAATCCACCACTGGCAATGGCAATGGCATTAACAAATGGTGCAAAACGACGCCGCATCCAGATGTTTGCATTCTCTTCATGTCACCGATCAAATCCCCACCCGAAAACCGACCGGAGTTTCGAAAAATGGCCATAAAAACCGCCATGGAGAAGGCCGCCGAGGCTCAAACTTACGCGGCCCGGTTCGGTCCGAGCTGCAAGACCACGCGCCAGAGAGCCGCGTGGGCCGACTGCTTGAAGCTCTACAACGACGCCGTTTTTCAACTGAACCGCACCCTCCAAGGCCTCGCCGGCCGAACCCACGGCCGCCACCGGTGTACGGACTTCGATGCACAAACGTGGCTGAGTTCTGCGCTCACTAACATCGACATGTGCCTCTCCGGCGCGGCGGATCTCAATGTGACGGAATTTATAACTCCGATCAGATGCCAGAAAGTTTCGAAGATGATTAGCAATTGTTTGGCGATTAATGGGAGGTTTTTGGAGGAAGGGGTTAAAAGTGGAGCAAAGGGAGTGGAAAATCAGAATGGGTTTCCGAGATGGGTTTCGAGTGGTGATCGGAAATTGTTGCAGGCGGCGAAGGTGAGGGCTAATTTGGTGGTGGCGAAAGATGGGTCTGGGAATTTCCGGTCGGTTCAGGCGGCGATCAACGCCGCCGCGAGGAGGCGGAGGAGGGGCCGATTTGTAATTTACGTAAAGCGAGGGGTTTACAGAGAGAATATTGAAGTTGGAACTAATAATGGGAATTTAATGCTGGTCGGAGATGGGATGAGATTTACAGTGATTACGAGTGGAAGAAGCGTCGCCGCCGGCTTCACCACCTTCAGTTCTGCAACCGCGGGTGGCTATTACTCTGTTCCAAATCCTTTTTAATTTAAGCATTTTTTCTTAAGAAAATTCGATGAAATTGTTTTTATATCTTTCAAAAAATTTTAAACTGTTTTAAATTAGCATTTTTTTTCCCTATATTCAGGAGTGATTCTATTTATTTATAAGTATGTTTTCTATAGTTCTTCAAAAATGAATTTAAAATTAATACAACTTTTTAAAGTGTTTTTCATTTGTTGATTTTTTTTTTTAAAAAAAAACCAATAATAAAAAAGCGTTCAATAAGTTGTACTAACTAAACGATTAAATTTTTTTAAAGTTACTTAAAAAGTCATTTTTCTTTTTAGTTTTTTCAAAAGAAATTGTTTCAAACATACTATCAGGGAATTGATAGAAGCAAGGAAGGAAAAATCAAGGGAATCAAGAGGGAGAAAGTAGAAACTCCCACTATGCTTGAAATTTGAAGAAGAAATTATAATTTTCTTTTTCTTTTCTTATGTGGTCTAATTTATCCTTTAAATTTCTTCCAATTTACTAAATCTCATGTTCTACCAAGTTTCAGTGACATAATATTTAGTAAATGAAAAAAAAAAATGAGAAATTAAGACAATTAAAGAAAAGTCAAATTTCAAAGAAAAAATTAGAAAAATTTCTTCTAAATAAATCTAGTTTCTTGTTTAAATTTCTATCACTTCATTAAGTATCATGCTCGTAAACTTACTATGAGTCACATTTAACTTTCAGGTATCCAAGGGCCAGCTTTCATAGCACGAGACATAGCCTTCGTGAACACGGCCGGTCCCCAGATGGGCCAAGCCGTGGCACTTCGGTCATCGTCGGACTTATCGGTGTTCCACCGGTGCAGCTTTCAGGGCTACCAAGACACACTACTGGTGCTCTCACAGAGACAATTCTACAAGCAATGTTACATCTATGGTACAATCGACTTCATTTTCGGCAACGCCGCAGTTGTTTTACAAAACTGCTTGATTTACGTACGAAGACCCTTAAAGGGTCAAGTCAACGTCATCACGGCCCAAGGTCGAGAGGACCCATTTCAAAACAGTGGAATCTCGATCCACAACTCTCAAATACGGGCCGCCGTCGATTTGCAGCCCATTATTGGGTCGGTCAAAACGTATTTGGGTCGGCCCTGGAAGAAGTATTCACGGACCGTGATCATGAGGAGTTATATTGACAAGTTGGTCAGCCCAATGGGCTGGCTAGCATGGCAAAATAGTAGATTTGCTCAAAATACGTTGTATTATGGGGAGTATAAGAATTTTGGGCCTAGGGCTTCGACCCGGTTTAGAGTGAGATGGCCCGGTTTTCATGCTATTAAAAACCCAAATGTGGCCGCTAAGTTTACCGTTAGGAGACTCATCGCCGGCCAAACTTGGCTACCGGCCACCGGAGTTCCGTTCAAGCTTGGCCTTTGA

mRNA sequence

ATGGATTCACAAAACAAAATTTCAATTTTTTCCATAACCCTTTTGGCAATTCTTTCAAATGTCTCTTCACAAGCAAAATCCACCACTGGCAATGGCAATGGCATTAACAAATGGTGCAAAACGACGCCGCATCCAGATGTTTGCATTCTCTTCATGTCACCGATCAAATCCCCACCCGAAAACCGACCGGAGTTTCGAAAAATGGCCATAAAAACCGCCATGGAGAAGGCCGCCGAGGCTCAAACTTACGCGGCCCGGTTCGGTCCGAGCTGCAAGACCACGCGCCAGAGAGCCGCGTGGGCCGACTGCTTGAAGCTCTACAACGACGCCGTTTTTCAACTGAACCGCACCCTCCAAGGCCTCGCCGGCCGAACCCACGGCCGCCACCGGTGTACGGACTTCGATGCACAAACGTGGCTGAGTTCTGCGCTCACTAACATCGACATGTGCCTCTCCGGCGCGGCGGATCTCAATGTGACGGAATTTATAACTCCGATCAGATGCCAGAAAGTTTCGAAGATGATTAGCAATTGTTTGGCGATTAATGGGAGGTTTTTGGAGGAAGGGGTTAAAAGTGGAGCAAAGGGAGTGGAAAATCAGAATGGGTTTCCGAGATGGGTTTCGAGTGGTGATCGGAAATTGTTGCAGGCGGCGAAGGTGAGGGCTAATTTGGTGGTGGCGAAAGATGGGTCTGGGAATTTCCGGTCGGTTCAGGCGGCGATCAACGCCGCCGCGAGGAGGCGGAGGAGGGGCCGATTTGTAATTTACGTAAAGCGAGGGGTTTACAGAGAGAATATTGAAGTTGGAACTAATAATGGGAATTTAATGCTGGTCGGAGATGGGATGAGATTTACAGTGATTACGAGTGGAAGAAGCGTCGCCGCCGGCTTCACCACCTTCAGTTCTGCAACCGCGGGTATCCAAGGGCCAGCTTTCATAGCACGAGACATAGCCTTCGTGAACACGGCCGGTCCCCAGATGGGCCAAGCCGTGGCACTTCGGTCATCGTCGGACTTATCGGTGTTCCACCGGTGCAGCTTTCAGGGCTACCAAGACACACTACTGGTGCTCTCACAGAGACAATTCTACAAGCAATGTTACATCTATGGTACAATCGACTTCATTTTCGGCAACGCCGCAGTTGTTTTACAAAACTGCTTGATTTACGTACGAAGACCCTTAAAGGGTCAAGTCAACGTCATCACGGCCCAAGGTCGAGAGGACCCATTTCAAAACAGTGGAATCTCGATCCACAACTCTCAAATACGGGCCGCCGTCGATTTGCAGCCCATTATTGGGTCGGTCAAAACGTATTTGGGTCGGCCCTGGAAGAAGTATTCACGGACCGTGATCATGAGGAGTTATATTGACAAGTTGGTCAGCCCAATGGGCTGGCTAGCATGGCAAAATAGTAGATTTGCTCAAAATACGTTGTATTATGGGGAGTATAAGAATTTTGGGCCTAGGGCTTCGACCCGGTTTAGAGTGAGATGGCCCGGTTTTCATGCTATTAAAAACCCAAATGTGGCCGCTAAGTTTACCGTTAGGAGACTCATCGCCGGCCAAACTTGGCTACCGGCCACCGGAGTTCCGTTCAAGCTTGGCCTTTGA

Coding sequence (CDS)

ATGGATTCACAAAACAAAATTTCAATTTTTTCCATAACCCTTTTGGCAATTCTTTCAAATGTCTCTTCACAAGCAAAATCCACCACTGGCAATGGCAATGGCATTAACAAATGGTGCAAAACGACGCCGCATCCAGATGTTTGCATTCTCTTCATGTCACCGATCAAATCCCCACCCGAAAACCGACCGGAGTTTCGAAAAATGGCCATAAAAACCGCCATGGAGAAGGCCGCCGAGGCTCAAACTTACGCGGCCCGGTTCGGTCCGAGCTGCAAGACCACGCGCCAGAGAGCCGCGTGGGCCGACTGCTTGAAGCTCTACAACGACGCCGTTTTTCAACTGAACCGCACCCTCCAAGGCCTCGCCGGCCGAACCCACGGCCGCCACCGGTGTACGGACTTCGATGCACAAACGTGGCTGAGTTCTGCGCTCACTAACATCGACATGTGCCTCTCCGGCGCGGCGGATCTCAATGTGACGGAATTTATAACTCCGATCAGATGCCAGAAAGTTTCGAAGATGATTAGCAATTGTTTGGCGATTAATGGGAGGTTTTTGGAGGAAGGGGTTAAAAGTGGAGCAAAGGGAGTGGAAAATCAGAATGGGTTTCCGAGATGGGTTTCGAGTGGTGATCGGAAATTGTTGCAGGCGGCGAAGGTGAGGGCTAATTTGGTGGTGGCGAAAGATGGGTCTGGGAATTTCCGGTCGGTTCAGGCGGCGATCAACGCCGCCGCGAGGAGGCGGAGGAGGGGCCGATTTGTAATTTACGTAAAGCGAGGGGTTTACAGAGAGAATATTGAAGTTGGAACTAATAATGGGAATTTAATGCTGGTCGGAGATGGGATGAGATTTACAGTGATTACGAGTGGAAGAAGCGTCGCCGCCGGCTTCACCACCTTCAGTTCTGCAACCGCGGGTATCCAAGGGCCAGCTTTCATAGCACGAGACATAGCCTTCGTGAACACGGCCGGTCCCCAGATGGGCCAAGCCGTGGCACTTCGGTCATCGTCGGACTTATCGGTGTTCCACCGGTGCAGCTTTCAGGGCTACCAAGACACACTACTGGTGCTCTCACAGAGACAATTCTACAAGCAATGTTACATCTATGGTACAATCGACTTCATTTTCGGCAACGCCGCAGTTGTTTTACAAAACTGCTTGATTTACGTACGAAGACCCTTAAAGGGTCAAGTCAACGTCATCACGGCCCAAGGTCGAGAGGACCCATTTCAAAACAGTGGAATCTCGATCCACAACTCTCAAATACGGGCCGCCGTCGATTTGCAGCCCATTATTGGGTCGGTCAAAACGTATTTGGGTCGGCCCTGGAAGAAGTATTCACGGACCGTGATCATGAGGAGTTATATTGACAAGTTGGTCAGCCCAATGGGCTGGCTAGCATGGCAAAATAGTAGATTTGCTCAAAATACGTTGTATTATGGGGAGTATAAGAATTTTGGGCCTAGGGCTTCGACCCGGTTTAGAGTGAGATGGCCCGGTTTTCATGCTATTAAAAACCCAAATGTGGCCGCTAAGTTTACCGTTAGGAGACTCATCGCCGGCCAAACTTGGCTACCGGCCACCGGAGTTCCGTTCAAGCTTGGCCTTTGA

Protein sequence

MDSQNKISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSPPENRPEFRKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRTHGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCLAINGRFLEEGVKSGAKGVENQNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAINAAARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYKNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL

Homology

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BLAST of Lag0019914 vs. NCBI nr
Match: XP_038905752.1 (probable pectinesterase/pectinesterase inhibitor 60 [Benincasa hispida])

HSP 1 Score: 886.3 bits (2289), Expect = 1.3e-253
Identity = 446/542 (82.29%), Postives = 481/542 (88.75%), Query Frame = 0


Query: 1   MDSQNKISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSPPE 60
           M S NKI  FSITL +ILSNVSS         N INKWCKTTPHP +CI FMSPIK PP+
Sbjct: 1   MASHNKILFFSITLFSILSNVSS---------NSINKWCKTTPHPKICIYFMSPIKPPPQ 60

Query: 61  NRPEFRKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQG 120
           NRPEFRKMAI+TA++KA EAQTYA RFGPSCKTTRQRAAW DCLKLYND VFQLNRTLQ 
Sbjct: 61  NRPEFRKMAIRTALQKATEAQTYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQC 120

Query: 121 LAGRTHGRH-RCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCL 180
           +      RH RCTDFDAQTWLSSALT+ID+C+SGAADLNVT+FI PI+CQ VSKMISNCL
Sbjct: 121 VISDGTIRHRRCTDFDAQTWLSSALTDIDLCVSGAADLNVTDFIAPIKCQNVSKMISNCL 180

Query: 181 AINGRFLEEGVKSGAK--GVENQNG--FPRWVSSGDRKLLQ-AAKVRANLVVAKDGSGNF 240
           A+NGRFLEE  K      GVE+     FP WVS GDRKLLQ +A+VRANLVVAKDGSG F
Sbjct: 181 AVNGRFLEEEAKKSETKIGVEDDRDGPFPMWVSDGDRKLLQSSARVRANLVVAKDGSGKF 240

Query: 241 RSVQAAINAAARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVA 300
           R+VQAAI+AAARRR RGRFVIYVKRGVYRENIEVG +NGNLMLVGDGMRFTVITSGRSVA
Sbjct: 241 RTVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVA 300

Query: 301 AGFTTFSSATAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTL 360
           AGFTTFSSATAGIQGP F+AR I FVNTAGP+MGQAVALRSSSDLSVFHRCSF+GYQDTL
Sbjct: 301 AGFTTFSSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTL 360

Query: 361 LVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSG 420
           +VLSQRQFYKQCY+YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSG
Sbjct: 361 MVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSG 420

Query: 421 ISIHNSQIRAAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFA 480
           ISIHNSQIRAA DL+P++GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ+S+FA
Sbjct: 421 ISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFA 480

Query: 481 QNTLYYGEYKNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKL 537
           Q TLYYGEY+N GPRA TR+RVRWPGFH IK+PNVA+KFTV+RLIAGQTWLPATGVPFKL
Sbjct: 481 QATLYYGEYRNIGPRAFTRYRVRWPGFHVIKSPNVASKFTVQRLIAGQTWLPATGVPFKL 533

[Close]

BLAST of Lag0019914 vs. NCBI nr
Match: KAG7015521.1 (putative pectinesterase/pectinesterase inhibitor 33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884.4 bits (2284), Expect = 4.9e-253
Identity = 440/532 (82.71%), Postives = 474/532 (89.10%), Query Frame = 0


Query: 6   KISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSPPENRPEF 65
           +I+ FSI L A+LSN SS    T  +GN INKWC TTPHP VCI FMSP+KSPP+NR EF
Sbjct: 6   QIAFFSIALFALLSNSSSSPTKT--SGNTINKWCNTTPHPKVCIFFMSPLKSPPQNRLEF 65

Query: 66  RKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRT 125
           RKMAIKT +E AA+AQ Y +RFGP+CKTTRQRAAW DC  LYND VFQLNRT+Q L G  
Sbjct: 66  RKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGN 125

Query: 126 HGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCLAINGRF 185
             RH CTDFDAQTWLSSALTNIDMC SGAADLNVT+FITPI+CQ VSKMISNCLAINGRF
Sbjct: 126 RLRH-CTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRF 185

Query: 186 LEEGVKSGAKGVEN-QNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAINAA 245
           L+E  KS   G E  QNGFP WVSSGDRKLLQ+A+VRA+LVVAKDGSG FR+VQAAINAA
Sbjct: 186 LQEETKSRTMGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAA 245

Query: 246 ARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSAT 305
           ARRR R RFVIY+KRGVY+ENIEVG +N N+MLVGDG+RFTVITSGRSVAAGFTTF+SAT
Sbjct: 246 ARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSAT 305

Query: 306 AGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYK 365
           AGIQGP F+AR I FVNTAGP+MGQAVALRSSSDLSVFHRCSF+GYQDTL+VLSQRQFYK
Sbjct: 306 AGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYK 365

Query: 366 QCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425
           QCY+YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA
Sbjct: 366 QCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425

Query: 426 AVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYK 485
           A DLQP+IGSVKTYLGRPWKKYSRTVIMRSYID LVSPMGWLAWQ +RFAQ+TLYYGEY+
Sbjct: 426 AADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYR 485

Query: 486 NFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           NFGPRASTR RVRWPGFHAI   NVA+KFTVRRLIAG TWLPATGVPFKL L
Sbjct: 486 NFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

[Close]

BLAST of Lag0019914 vs. NCBI nr
Match: XP_022932129.1 (probable pectinesterase/pectinesterase inhibitor 33 [Cucurbita moschata])

HSP 1 Score: 883.6 bits (2282), Expect = 8.3e-253
Identity = 440/532 (82.71%), Postives = 474/532 (89.10%), Query Frame = 0


Query: 6   KISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSPPENRPEF 65
           +I+ FSI L A+LSN SS    T  +GN INKWC TTPHP VCI FMSP+KSPP+NR EF
Sbjct: 6   QIAFFSIALFALLSNSSSSPTKT--SGNTINKWCNTTPHPKVCIFFMSPLKSPPQNRLEF 65

Query: 66  RKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRT 125
           RKMAIKT +E AA+AQ Y + FGP+CKTTRQRAAW DC  LYND VFQLNRT+Q L G T
Sbjct: 66  RKMAIKTTLEGAAKAQNYVSLFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGT 125

Query: 126 HGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCLAINGRF 185
             RH CTDFDAQTWLSSALTNIDMC SGAADLNVT+FITPI+CQ VSKMISNCLAINGRF
Sbjct: 126 RLRH-CTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRF 185

Query: 186 LEEGVKSGAKGVEN-QNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAINAA 245
           L+E  KS   G E  QNGFP WVSSGDRKLLQ+A+VRA+LVVAKDGSG FR+VQAAINAA
Sbjct: 186 LQEETKSRTTGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAA 245

Query: 246 ARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSAT 305
           ARRR R RFVIY+KRGVY+ENIEVG +N N+MLVGDG+RFTVITSGRSVAAGFTTF+SAT
Sbjct: 246 ARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSAT 305

Query: 306 AGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYK 365
           AGIQGP F+AR I FVNTAGP+MGQAVALRSSSDLSVFHRCSF+GYQDTL+VLSQRQFYK
Sbjct: 306 AGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYK 365

Query: 366 QCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425
           QCY+YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA
Sbjct: 366 QCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425

Query: 426 AVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYK 485
           A DLQP+IGSVKTYLGRPWKKYSRTVIMRSYID LVSPMGWLAWQ +RFAQ+TLYYGEY+
Sbjct: 426 AADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYR 485

Query: 486 NFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           NFGPRASTR RVRWPGFHAI   NVA+KFTVRRLIAG TWLPATGVPFKL L
Sbjct: 486 NFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

[Close]

BLAST of Lag0019914 vs. NCBI nr
Match: XP_022985199.1 (probable pectinesterase/pectinesterase inhibitor 33 [Cucurbita maxima])

HSP 1 Score: 880.6 bits (2274), Expect = 7.1e-252
Identity = 438/532 (82.33%), Postives = 473/532 (88.91%), Query Frame = 0


Query: 6   KISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSPPENRPEF 65
           +I+ FSI L A+LSN SS    T  + N INKWC TTPHP VCI FMSP+KSPP+NR EF
Sbjct: 6   QIAFFSIALFALLSNSSSSPTKT--SANTINKWCNTTPHPKVCIFFMSPLKSPPQNRIEF 65

Query: 66  RKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRT 125
           RKMAIKT +E AA+AQ Y +RFGP+CKTTRQRAAW DC  LYND VFQLNRT++ L   T
Sbjct: 66  RKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGT 125

Query: 126 HGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCLAINGRF 185
             RH CTDFDAQTWLSSALTNIDMC SGAADLNVT+FITPI+CQ VS+MISNCLAINGRF
Sbjct: 126 RLRH-CTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGRF 185

Query: 186 LEEGVKSGAKGVEN-QNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAINAA 245
           LE   KS   G E  QNGFPRWVSSGDRKLLQ+A+VRA+LVVAKDGSG FR+VQAAINAA
Sbjct: 186 LEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAA 245

Query: 246 ARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSAT 305
           ARRR R RFVIY+KRGVYRENIE+G +N N+MLVGDG+RFTVITSGRSVAAGFTTF+SAT
Sbjct: 246 ARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSAT 305

Query: 306 AGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYK 365
           AGIQGP F+AR I FVNTAGP+MGQAVALRSSSDLSVFHRCSF+GYQDTL+VLSQRQFYK
Sbjct: 306 AGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYK 365

Query: 366 QCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425
           QCY+YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA
Sbjct: 366 QCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425

Query: 426 AVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYK 485
           A DLQP+IGSVKTYLGRPWKKYSRTVIMRSYID LVSPMGWLAWQ +RFAQ+TLYYGEY+
Sbjct: 426 AADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYR 485

Query: 486 NFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           NFGPRASTR RVRWPGFHAI   NVA+KFTVRRLIAG TWLPATGVPFKL L
Sbjct: 486 NFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

[Close]

BLAST of Lag0019914 vs. NCBI nr
Match: XP_023520442.1 (probable pectinesterase/pectinesterase inhibitor 59 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 878.2 bits (2268), Expect = 3.5e-251
Identity = 436/532 (81.95%), Postives = 473/532 (88.91%), Query Frame = 0


Query: 6   KISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSPPENRPEF 65
           +I+ FSI L A+LSN SS    T  + N INKWC TTPHP VCI FMSP+KSPP+NR +F
Sbjct: 56  QIAFFSIALFALLSNSSSSLTKT--SANTINKWCNTTPHPKVCIFFMSPLKSPPQNRLQF 115

Query: 66  RKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRT 125
           RK+AIKT +E AA+AQ Y +RFGP+CKTTRQRAAW DC  LYND VFQLNRT+Q L   T
Sbjct: 116 RKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDAGT 175

Query: 126 HGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCLAINGRF 185
             RH CTDFDAQTWLSSALTNIDMC SGAADLNVT+FITPI+CQ VSKMISNCLAINGRF
Sbjct: 176 RLRH-CTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRF 235

Query: 186 LEEGVKSGAKGVEN-QNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAINAA 245
           LEE  KS   G E  QNGFP W+S+GDRKLLQ+A+VRA+LVVAKDGSG FR+VQAAINAA
Sbjct: 236 LEEETKSRTTGTEEYQNGFPTWLSTGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAA 295

Query: 246 ARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSAT 305
           ARRR R RFVIY+KRGVY+ENIEVG +N N+MLVGDG+RFTVITSGRSVAAGFTTF+SAT
Sbjct: 296 ARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSAT 355

Query: 306 AGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYK 365
           AGIQGP F+AR I FVNTAGP+MGQAVALRSSSDLSVFHRCSF+GYQDTL+VLSQRQFYK
Sbjct: 356 AGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYK 415

Query: 366 QCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425
           QCY+YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA
Sbjct: 416 QCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 475

Query: 426 AVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYK 485
           A DLQP+IGSVKTYLGRPWKKYSRTVIMRSYID LVSPMGWLAWQ +RFAQ+TLYYGEY+
Sbjct: 476 AADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYR 535

Query: 486 NFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           NFGPRASTR RVRWPGFHAI   NVA+KFTVRRLIAG TWLPATGVPFKL L
Sbjct: 536 NFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 584

[Close]

BLAST of Lag0019914 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 5.9e-169
Identity = 303/536 (56.53%), Postives = 387/536 (72.20%), Query Frame = 0


Query: 6   KISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIK--SPPENRP 65
           K+SI  + L+ +L  +     +T  + N  + WC  TP+PD C  +         P    
Sbjct: 7   KLSILFLHLI-LLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQLS 66

Query: 66  EFRKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAG 125
           EFR M ++ AM++A  A+      G +C  ++++A  ADC+ LY D + QLNRTL G++ 
Sbjct: 67  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 126

Query: 126 RTHGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQ-KVSKMISNCLAIN 185
           +      CTDFDAQTWLS+ALTN + C  G++DLNVT+FITPI    K+S +ISNCLA+N
Sbjct: 127 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 186

Query: 186 GRFLEEGVKSGAKGVENQNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAIN 245
           G  L  G K       NQ GFP W+S  D++LL+A  VRANLVVAKDGSG+F +VQAAI+
Sbjct: 187 GALLTAGNKGNT--TANQKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNTVQAAID 246

Query: 246 AAARRR-RRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFS 305
            A RR+   GRFVIYVKRG+Y+ENI V  NN ++MLVGDGMR T+IT GRSV  G+TT++
Sbjct: 247 VAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYN 306

Query: 306 SATAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQ 365
           SATAGI+G  FIA+ I F NTAGP  GQAVALRSSSDLS+F++CS +GYQDTL+V SQRQ
Sbjct: 307 SATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQ 366

Query: 366 FYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQ 425
           FY++CYIYGT+DFIFGNAA V QNCLI  RRPLKGQ NVITAQGR DPFQN+GISIHNS+
Sbjct: 367 FYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSR 426

Query: 426 IRAAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAW-QNSRFAQNTLYY 485
           I  A DL+P++G+VKTY+GRPW K+SRTV++++Y+D +VSP+GW  W + S F  +TL+Y
Sbjct: 427 ILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFY 486

Query: 486 GEYKNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
            EYKN GP +STR+RV W GFH +   + A+ FTV + IAG  WLP TG+PF  GL
Sbjct: 487 AEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of Lag0019914 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.5e-167
Identity = 306/534 (57.30%), Postives = 383/534 (71.72%), Query Frame = 0


Query: 7   ISIFSITLLAILSNVSSQAKSTT-GNGNGINKWCKTTPHPDVCILFMSPIKSP----PEN 66
           IS  S+ LL IL        +   GN   I+ WC  TP+P  C  +   IK      P  
Sbjct: 9   ISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYF--IKHSGFRLPTQ 68

Query: 67  RPEFRKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGL 126
             EFR + ++ AM++A  A         +C   +++A  ADC+ LY D V QLNRTLQG+
Sbjct: 69  ISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGV 128

Query: 127 AGRTHGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQ-KVSKMISNCLA 186
           + +T GR RCTDFDAQTWLS+ALTN + C  G++DLNV++F TPI    K+S +ISNCLA
Sbjct: 129 SSKT-GR-RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLA 188

Query: 187 INGRFLEEGVKSGAKGVENQNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAA 246
           +NG  L  G      G  +  GFP WVS  +R+LLQ   VRANLVVAKDGSG+F++VQAA
Sbjct: 189 VNGALLTAGKNDSTTG--DSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAA 248

Query: 247 INAAARRR-RRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTT 306
           I+ A RR+   GRFVIYVKRG+Y+EN+ V  NN N+MLVGDGMR+T+IT GRSV  G+TT
Sbjct: 249 IDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTT 308

Query: 307 FSSATAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQ 366
           +SSATAGI+G  FIA+ IAF NTAGP  GQAVALRSSSDLS+F+RCS +GYQDTL+V SQ
Sbjct: 309 YSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQ 368

Query: 367 RQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHN 426
           RQFY++CYIYGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIHN
Sbjct: 369 RQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHN 428

Query: 427 SQIRAAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAW-QNSRFAQNTL 486
           S I  A DL+P++ SVKTY+GRPW  YSRTV++++YID +VSP+GW  W + S +  +TL
Sbjct: 429 SIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTL 488

Query: 487 YYGEYKNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPF 533
           +Y EYKN GP +STR+RVRW GFH +   + A+ F+V + IAG  WLP +G+PF
Sbjct: 489 FYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

[Close]

BLAST of Lag0019914 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 7.3e-167
Identity = 296/526 (56.27%), Postives = 380/526 (72.24%), Query Frame = 0


Query: 25  AKSTTGNGN---GINKWCKTTPHPDVCILFMSPIKS------PPENRPEFRKMAIKTAME 84
           A S   NG+    +  WC  TPH + C  +            PP  R EF +M +K A++
Sbjct: 71  APSPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALD 130

Query: 85  KAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRTHGRHRCTDFD 144
           +A    +   +FGPSC   +++AAW+DC+ L+ + V QLNRTL+GL        +CTDFD
Sbjct: 131 QAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFD 190

Query: 145 AQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQK-VSKMISNCLAINGRFLEEGVKSGA 204
           AQTWLS+A TNI+ C SG+ DLNV++F+ P+   K +S +I NCLA+NG  +++      
Sbjct: 191 AQTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQ--HDHT 250

Query: 205 KGVENQNGFPRWVSSGDRKLL---QAAKVRANLVVAKDGSGNFRSVQAAINAAARRRRRG 264
               ++  FP WVS  +R+LL     AK   +LVVA+D SG+FRS+QAAIN AARRR + 
Sbjct: 251 TTANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKS 310

Query: 265 RFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPA 324
           RFVIYVK+GVYRENI+VG +N N+MLVGDG R T+ITSGRSV  G+TT++SAT G  G  
Sbjct: 311 RFVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQR 370

Query: 325 FIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYKQCYIYGT 384
           F+A+D+ F+NTAGP  GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CYI GT
Sbjct: 371 FVAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGT 430

Query: 385 IDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAVDLQPI 444
           IDFIFGNAAVV QNC+I VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA DL+P+
Sbjct: 431 IDFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPV 490

Query: 445 IGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAW-QNSRFAQNTLYYGEYKNFGPRA 504
           I + KTYLGRPW+ YSR  IM++YID  +SP+GW  W + S FA NT++YGEYKNFGP +
Sbjct: 491 IRAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGS 550

Query: 505 STRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           STR+RVRW GFHAI +  VA++FTV  LIAG +WLPATGVPFK GL
Sbjct: 551 STRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of Lag0019914 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 493.0 bits (1268), Expect = 4.2e-138
Identity = 261/529 (49.34%), Postives = 349/529 (65.97%), Query Frame = 0


Query: 10  FSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFM--SPIKSPPENRPEFRK 69
           F I  + + S VS        N   +  WC  TP+P  C  F+  +    P ++  EF K
Sbjct: 11  FVILCILVASTVSGY------NQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLK 70

Query: 70  MAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRTHG 129
           +++K  +++A  A+T+A   GP C+ TR++AAW DC+KLY+  V ++N T+         
Sbjct: 71  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD-------P 130

Query: 130 RHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCLAINGRFLE 189
             +C+  DAQTWLS+ALTN+D C +G  +L VT+ + P+    VS ++ N LAIN     
Sbjct: 131 NVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAIN----- 190

Query: 190 EGVKSGAKGVENQNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAINAAARR 249
             V       E ++GFP WV  GDRKLLQ++  + N VVAKDGSGNF++++ AI+AA+  
Sbjct: 191 -KVPFNYTPPE-KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS-- 250

Query: 250 RRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI 309
              GRFVIYVK+GVY EN+E+     N+ML GDG+  T+IT  +SV  G TTF+SAT   
Sbjct: 251 -GSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAA 310

Query: 310 QGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYKQCY 369
            G  FIAR I F NTAG    QAVALRS SDLSVF++CSF+ YQDTL V S RQFY+ C 
Sbjct: 311 VGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCD 370

Query: 370 IYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAVD 429
           +YGT+DFIFGNAA VLQNC I+ RRP + + N ITAQGR DP QN+GI IHNS++ AA D
Sbjct: 371 VYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASD 430

Query: 430 LQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYKNFG 489
           L+P++GS KTYLGRPW++YSRTV M++ +D L+ P GWL W +  FA  TL+Y E++N G
Sbjct: 431 LRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEW-DGNFALKTLFYAEFQNTG 490

Query: 490 PRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           P AST  RV WPGF  + + + A+KFTV   +AG +W+P++ VPF  GL
Sbjct: 491 PGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

[Close]

BLAST of Lag0019914 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.6e-137
Identity = 254/504 (50.40%), Postives = 334/504 (66.27%), Query Frame = 0


Query: 35  INKWCKTTPHPDVCILFMSPIK--SPPENRPEFRKMAIKTAMEKAAEAQTYAARFGPSCK 94
           +  WC  TP+P  C  F++     +  +   +F K++++ A+E+A  AQ+     G  C+
Sbjct: 28  VKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSRTYTLGSKCR 87

Query: 95  TTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRTHGRHRCTDFDAQTWLSSALTNIDMCLS 154
             R++AAW DC +LY   V +LN+T     G       CT  D QTWLS+ALTN++ C +
Sbjct: 88  NEREKAAWEDCRELYELTVLKLNQTSNSSPG-------CTKVDKQTWLSTALTNLETCRA 147

Query: 155 GAADLNVTEFITPIRCQKVSKMISNCLAINGRFLEEGVKSGAKGVENQNGFPRWVSSGDR 214
              DL V E++ P+    V+K+ISN L++N        K        ++GFP WV  GDR
Sbjct: 148 SLEDLGVPEYVLPLLSNNVTKLISNTLSLN--------KVPYNEPSYKDGFPTWVKPGDR 207

Query: 215 KLLQAAKVRANLVVAKDGSGNFRSVQAAINAAARRRRRGRFVIYVKRGVYRENIEVGTNN 274
           KLLQ    RAN+VVA+DGSGN +++Q A+ AAA R    R+VIY+K G Y ENIEV    
Sbjct: 208 KLLQTTP-RANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYVIYIKAGTYNENIEVKLK- 267

Query: 275 GNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPAFIARDIAFVNTAGPQMGQAVA 334
            N+M VGDG+  T+IT  +SV  G TTF SAT  + G  FIARDI   NTAGP   QAVA
Sbjct: 268 -NIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVA 327

Query: 335 LRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRR 394
           LRS SDLSVF+RCSF+GYQDTL V SQRQFY++C IYGT+DFIFGNAAVVLQNC I+ R+
Sbjct: 328 LRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARK 387

Query: 395 PLKGQVNVITAQGREDPFQNSGISIHNSQIRAAVDLQPIIGSVKTYLGRPWKKYSRTVIM 454
           P   + N +TAQGR DP Q++GI IHN ++ AA DL+P+  SVKT+LGRPWK+YSRTV +
Sbjct: 388 P-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYI 447

Query: 455 RSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYKNFGPRASTRFRVRWPGFHAIKNPNVAAK 514
           ++++D L++P GW+ W    FA NTLYY EY N GP +ST  RV+W G+H + +P+  ++
Sbjct: 448 KTFLDSLINPAGWMEWSGD-FALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQ 507

Query: 515 FTVRRLIAGQTWLPATGVPFKLGL 537
           FTV   IAG +WLPAT VPF  GL
Sbjct: 508 FTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of Lag0019914 vs. ExPASy TrEMBL
Match: A0A6J1EW59 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438452 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 4.0e-253
Identity = 440/532 (82.71%), Postives = 474/532 (89.10%), Query Frame = 0


Query: 6   KISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSPPENRPEF 65
           +I+ FSI L A+LSN SS    T  +GN INKWC TTPHP VCI FMSP+KSPP+NR EF
Sbjct: 6   QIAFFSIALFALLSNSSSSPTKT--SGNTINKWCNTTPHPKVCIFFMSPLKSPPQNRLEF 65

Query: 66  RKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRT 125
           RKMAIKT +E AA+AQ Y + FGP+CKTTRQRAAW DC  LYND VFQLNRT+Q L G T
Sbjct: 66  RKMAIKTTLEGAAKAQNYVSLFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGT 125

Query: 126 HGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCLAINGRF 185
             RH CTDFDAQTWLSSALTNIDMC SGAADLNVT+FITPI+CQ VSKMISNCLAINGRF
Sbjct: 126 RLRH-CTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRF 185

Query: 186 LEEGVKSGAKGVEN-QNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAINAA 245
           L+E  KS   G E  QNGFP WVSSGDRKLLQ+A+VRA+LVVAKDGSG FR+VQAAINAA
Sbjct: 186 LQEETKSRTTGTEEYQNGFPTWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAA 245

Query: 246 ARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSAT 305
           ARRR R RFVIY+KRGVY+ENIEVG +N N+MLVGDG+RFTVITSGRSVAAGFTTF+SAT
Sbjct: 246 ARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSAT 305

Query: 306 AGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYK 365
           AGIQGP F+AR I FVNTAGP+MGQAVALRSSSDLSVFHRCSF+GYQDTL+VLSQRQFYK
Sbjct: 306 AGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYK 365

Query: 366 QCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425
           QCY+YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA
Sbjct: 366 QCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425

Query: 426 AVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYK 485
           A DLQP+IGSVKTYLGRPWKKYSRTVIMRSYID LVSPMGWLAWQ +RFAQ+TLYYGEY+
Sbjct: 426 AADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYR 485

Query: 486 NFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           NFGPRASTR RVRWPGFHAI   NVA+KFTVRRLIAG TWLPATGVPFKL L
Sbjct: 486 NFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

[Close]

BLAST of Lag0019914 vs. ExPASy TrEMBL
Match: A0A6J1JCM7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483275 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.4e-252
Identity = 438/532 (82.33%), Postives = 473/532 (88.91%), Query Frame = 0


Query: 6   KISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSPPENRPEF 65
           +I+ FSI L A+LSN SS    T  + N INKWC TTPHP VCI FMSP+KSPP+NR EF
Sbjct: 6   QIAFFSIALFALLSNSSSSPTKT--SANTINKWCNTTPHPKVCIFFMSPLKSPPQNRIEF 65

Query: 66  RKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRT 125
           RKMAIKT +E AA+AQ Y +RFGP+CKTTRQRAAW DC  LYND VFQLNRT++ L   T
Sbjct: 66  RKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGT 125

Query: 126 HGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCLAINGRF 185
             RH CTDFDAQTWLSSALTNIDMC SGAADLNVT+FITPI+CQ VS+MISNCLAINGRF
Sbjct: 126 RLRH-CTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGRF 185

Query: 186 LEEGVKSGAKGVEN-QNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAINAA 245
           LE   KS   G E  QNGFPRWVSSGDRKLLQ+A+VRA+LVVAKDGSG FR+VQAAINAA
Sbjct: 186 LEGETKSRTTGAEEYQNGFPRWVSSGDRKLLQSARVRAHLVVAKDGSGKFRTVQAAINAA 245

Query: 246 ARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSAT 305
           ARRR R RFVIY+KRGVYRENIE+G +N N+MLVGDG+RFTVITSGRSVAAGFTTF+SAT
Sbjct: 246 ARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSAT 305

Query: 306 AGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYK 365
           AGIQGP F+AR I FVNTAGP+MGQAVALRSSSDLSVFHRCSF+GYQDTL+VLSQRQFYK
Sbjct: 306 AGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYK 365

Query: 366 QCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425
           QCY+YGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA
Sbjct: 366 QCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRA 425

Query: 426 AVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYK 485
           A DLQP+IGSVKTYLGRPWKKYSRTVIMRSYID LVSPMGWLAWQ +RFAQ+TLYYGEY+
Sbjct: 426 AADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYR 485

Query: 486 NFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           NFGPRASTR RVRWPGFHAI   NVA+KFTVRRLIAG TWLPATGVPFKL L
Sbjct: 486 NFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

[Close]

BLAST of Lag0019914 vs. ExPASy TrEMBL
Match: A0A1S3BUR4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493398 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.2e-241
Identity = 435/543 (80.11%), Postives = 467/543 (86.00%), Query Frame = 0


Query: 1   MDSQNKISIF-SITLL-AILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSP 60
           M S  KI +F SITLL  ILSN            N I KWCKTTPHP VCI FMSPIK P
Sbjct: 1   MASHYKIILFISITLLFTILSN-------NVSANNPIIKWCKTTPHPKVCISFMSPIKPP 60

Query: 61  PENRPEFRKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTL 120
           P+NR EFR++AI+T +EKA EA+ Y ARFGP+CKTTRQR AW DC KLYND VFQLNRTL
Sbjct: 61  PQNREEFRQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTL 120

Query: 121 QGLA--GRTHGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMIS 180
           Q L      H R  CTDFDAQTWLSSALT+ID+C SGAADLNVT+FI PI+C  VSKMIS
Sbjct: 121 QCLVTDETIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMIS 180

Query: 181 NCLAINGRFLEEGVKSGAKGVENQNG-FPRWVSSGDRKLLQA--AKVRANLVVAKDGSGN 240
           NCLAING FLEE    G K  +  NG FP WVS GDRKLL++   +VRANLVVAKDGSG 
Sbjct: 181 NCLAINGGFLEE---EGVKYDDGGNGSFPMWVSEGDRKLLESRPRRVRANLVVAKDGSGK 240

Query: 241 FRSVQAAINAAARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSV 300
           FR VQAAI+AAARRR RGRFVIYVKRGVYRENIEVG +NGN+MLVGDGMRFTVITSGRSV
Sbjct: 241 FRRVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSV 300

Query: 301 AAGFTTFSSATAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDT 360
           AAGFTTFSSATAGIQGP FIARDI FVNTAGP+MGQAVALRSSSDLSVFHRCSF+GYQDT
Sbjct: 301 AAGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDT 360

Query: 361 LLVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNS 420
           L+VLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNS
Sbjct: 361 LMVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNS 420

Query: 421 GISIHNSQIRAAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRF 480
           GISIHNSQIRAA DL+P++GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ+S+F
Sbjct: 421 GISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKF 480

Query: 481 AQNTLYYGEYKNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFK 537
           AQ TLYYGEY N GPRASTRFRV+WPGFH IK+PNVA+KF+VRRLIAGQTWLPATGVPFK
Sbjct: 481 AQATLYYGEYHNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFK 533

[Close]

BLAST of Lag0019914 vs. ExPASy TrEMBL
Match: A0A0A0L330 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G088980 PE=3 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 6.7e-240
Identity = 424/540 (78.52%), Postives = 464/540 (85.93%), Query Frame = 0


Query: 1   MDSQNKISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIKSPPE 60
           M S  KI   SIT   +LSN  S         N I KWCKTTPHP VC+  MSPIK PP+
Sbjct: 1   MASHYKILFISITFFTLLSNNVS--------ANPIIKWCKTTPHPRVCVSLMSPIKPPPQ 60

Query: 61  NRPEFRKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQ- 120
           NR EFR+MAI+T +EKAAEA+ Y ARFGP+CKT+RQR AW DC KLYND V QLNRTL  
Sbjct: 61  NRREFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHC 120

Query: 121 GLAGRTHGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCL 180
            +      R  CTDFDAQTWLSSALT+ID+C SGAADLNVT+FITPI+C  VSKMISNCL
Sbjct: 121 VVTDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCL 180

Query: 181 AINGRFLEEGVKSGAKGVENQNG-FPRWVSSGDRKLLQA--AKVRANLVVAKDGSGNFRS 240
           AING FLEE    G K  + +NG FP WVS GDRKLL++   +VRANLVVAKDGSG FR 
Sbjct: 181 AINGGFLEE---EGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRR 240

Query: 241 VQAAINAAARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAG 300
           VQAAI+AAARRR RGRF+IYVKRGVYRENIEVG +NGN+MLVGDGMRFTVITSGRSVAAG
Sbjct: 241 VQAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAG 300

Query: 301 FTTFSSATAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLV 360
           FTTFSSATAGIQGP FIARDI FVNTAGP+MGQAVALRSSSDLSVFHRCSF+GYQDTL+V
Sbjct: 301 FTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMV 360

Query: 361 LSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGIS 420
           LSQRQFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGIS
Sbjct: 361 LSQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGIS 420

Query: 421 IHNSQIRAAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQN 480
           IHNSQIRAA DL+P++GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ+S+FAQ 
Sbjct: 421 IHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQA 480

Query: 481 TLYYGEYKNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           TLYYGEY+N GPRASTRFRV+WPGFH IK+PNVA+KF+V+RLIAGQTWLPATGVPFKLG+
Sbjct: 481 TLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 529

[Close]

BLAST of Lag0019914 vs. ExPASy TrEMBL
Match: A0A5A7TVK4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G001080 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 3.2e-226
Identity = 400/490 (81.63%), Postives = 433/490 (88.37%), Query Frame = 0


Query: 52  MSPIKSPPENRPEFRKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAV 111
           MSPIK PP+NR EFR++AI+T +EKA EA+ Y ARFGP+CKTTRQR AW DC KLYND V
Sbjct: 1   MSPIKPPPQNREEFRQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVV 60

Query: 112 FQLNRTLQGLA--GRTHGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQ 171
           FQLNRTLQ L      H R  CTDFDAQTWLSSALT+ID+C SGAADLNVT+FI PI+C 
Sbjct: 61  FQLNRTLQCLVTDETIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCL 120

Query: 172 KVSKMISNCLAINGRFLEEGVKSGAKGVENQNG-FPRWVSSGDRKLLQA--AKVRANLVV 231
            VSKMISNCLAING F+EE    G K  +  NG FP WVS GDRKLL++   +VRANLVV
Sbjct: 121 NVSKMISNCLAINGGFIEE---EGVKYDDGGNGSFPMWVSEGDRKLLESRPRRVRANLVV 180

Query: 232 AKDGSGNFRSVQAAINAAARRRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTV 291
           AKDGSG FR VQAAI+AAARRR RGRFVIYVKRGVYRENIEVG +NGN+MLVGDGMRFTV
Sbjct: 181 AKDGSGKFRRVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTV 240

Query: 292 ITSGRSVAAGFTTFSSATAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCS 351
           ITSGRSVAAGFTTFS     IQGP FIARDI FVNTAGP+MGQAVALRSSSDLSVFHRCS
Sbjct: 241 ITSGRSVAAGFTTFS-----IQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCS 300

Query: 352 FQGYQDTLLVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGR 411
           F+GYQDTL+VLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGR
Sbjct: 301 FEGYQDTLMVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGR 360

Query: 412 EDPFQNSGISIHNSQIRAAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWL 471
           EDPFQNSGISIHNSQIRAA DL+P++GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWL
Sbjct: 361 EDPFQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWL 420

Query: 472 AWQNSRFAQNTLYYGEYKNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLP 531
           AWQ+S+FAQ TLYYGEY+N GPRASTRFRV+WPGFH IK+PNVA+KF+VRRLIAGQTWLP
Sbjct: 421 AWQSSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLP 480

Query: 532 ATGVPFKLGL 537
           ATGVPFKLG+
Sbjct: 481 ATGVPFKLGV 482

[Close]

BLAST of Lag0019914 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 595.5 bits (1534), Expect = 4.2e-170
Identity = 303/536 (56.53%), Postives = 387/536 (72.20%), Query Frame = 0


Query: 6   KISIFSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFMSPIK--SPPENRP 65
           K+SI  + L+ +L  +     +T  + N  + WC  TP+PD C  +         P    
Sbjct: 7   KLSILFLHLI-LLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQLS 66

Query: 66  EFRKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAG 125
           EFR M ++ AM++A  A+      G +C  ++++A  ADC+ LY D + QLNRTL G++ 
Sbjct: 67  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 126

Query: 126 RTHGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQ-KVSKMISNCLAIN 185
           +      CTDFDAQTWLS+ALTN + C  G++DLNVT+FITPI    K+S +ISNCLA+N
Sbjct: 127 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 186

Query: 186 GRFLEEGVKSGAKGVENQNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAIN 245
           G  L  G K       NQ GFP W+S  D++LL+A  VRANLVVAKDGSG+F +VQAAI+
Sbjct: 187 GALLTAGNKGNT--TANQKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNTVQAAID 246

Query: 246 AAARRR-RRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFS 305
            A RR+   GRFVIYVKRG+Y+ENI V  NN ++MLVGDGMR T+IT GRSV  G+TT++
Sbjct: 247 VAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYN 306

Query: 306 SATAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQ 365
           SATAGI+G  FIA+ I F NTAGP  GQAVALRSSSDLS+F++CS +GYQDTL+V SQRQ
Sbjct: 307 SATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQ 366

Query: 366 FYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQ 425
           FY++CYIYGT+DFIFGNAA V QNCLI  RRPLKGQ NVITAQGR DPFQN+GISIHNS+
Sbjct: 367 FYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSR 426

Query: 426 IRAAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAW-QNSRFAQNTLYY 485
           I  A DL+P++G+VKTY+GRPW K+SRTV++++Y+D +VSP+GW  W + S F  +TL+Y
Sbjct: 427 ILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFY 486

Query: 486 GEYKNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
            EYKN GP +STR+RV W GFH +   + A+ FTV + IAG  WLP TG+PF  GL
Sbjct: 487 AEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of Lag0019914 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 590.1 bits (1520), Expect = 1.8e-168
Identity = 306/534 (57.30%), Postives = 383/534 (71.72%), Query Frame = 0


Query: 7   ISIFSITLLAILSNVSSQAKSTT-GNGNGINKWCKTTPHPDVCILFMSPIKSP----PEN 66
           IS  S+ LL IL        +   GN   I+ WC  TP+P  C  +   IK      P  
Sbjct: 9   ISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYF--IKHSGFRLPTQ 68

Query: 67  RPEFRKMAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGL 126
             EFR + ++ AM++A  A         +C   +++A  ADC+ LY D V QLNRTLQG+
Sbjct: 69  ISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGV 128

Query: 127 AGRTHGRHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQ-KVSKMISNCLA 186
           + +T GR RCTDFDAQTWLS+ALTN + C  G++DLNV++F TPI    K+S +ISNCLA
Sbjct: 129 SSKT-GR-RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLA 188

Query: 187 INGRFLEEGVKSGAKGVENQNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAA 246
           +NG  L  G      G  +  GFP WVS  +R+LLQ   VRANLVVAKDGSG+F++VQAA
Sbjct: 189 VNGALLTAGKNDSTTG--DSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAA 248

Query: 247 INAAARRR-RRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTT 306
           I+ A RR+   GRFVIYVKRG+Y+EN+ V  NN N+MLVGDGMR+T+IT GRSV  G+TT
Sbjct: 249 IDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTT 308

Query: 307 FSSATAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQ 366
           +SSATAGI+G  FIA+ IAF NTAGP  GQAVALRSSSDLS+F+RCS +GYQDTL+V SQ
Sbjct: 309 YSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQ 368

Query: 367 RQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHN 426
           RQFY++CYIYGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIHN
Sbjct: 369 RQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHN 428

Query: 427 SQIRAAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAW-QNSRFAQNTL 486
           S I  A DL+P++ SVKTY+GRPW  YSRTV++++YID +VSP+GW  W + S +  +TL
Sbjct: 429 SIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTL 488

Query: 487 YYGEYKNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPF 533
           +Y EYKN GP +STR+RVRW GFH +   + A+ F+V + IAG  WLP +G+PF
Sbjct: 489 FYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

[Close]

BLAST of Lag0019914 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 588.6 bits (1516), Expect = 5.2e-168
Identity = 296/526 (56.27%), Postives = 380/526 (72.24%), Query Frame = 0


Query: 25  AKSTTGNGN---GINKWCKTTPHPDVCILFMSPIKS------PPENRPEFRKMAIKTAME 84
           A S   NG+    +  WC  TPH + C  +            PP  R EF +M +K A++
Sbjct: 71  APSPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALD 130

Query: 85  KAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRTHGRHRCTDFD 144
           +A    +   +FGPSC   +++AAW+DC+ L+ + V QLNRTL+GL        +CTDFD
Sbjct: 131 QAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFD 190

Query: 145 AQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQK-VSKMISNCLAINGRFLEEGVKSGA 204
           AQTWLS+A TNI+ C SG+ DLNV++F+ P+   K +S +I NCLA+NG  +++      
Sbjct: 191 AQTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQ--HDHT 250

Query: 205 KGVENQNGFPRWVSSGDRKLL---QAAKVRANLVVAKDGSGNFRSVQAAINAAARRRRRG 264
               ++  FP WVS  +R+LL     AK   +LVVA+D SG+FRS+QAAIN AARRR + 
Sbjct: 251 TTANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKS 310

Query: 265 RFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPA 324
           RFVIYVK+GVYRENI+VG +N N+MLVGDG R T+ITSGRSV  G+TT++SAT G  G  
Sbjct: 311 RFVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQR 370

Query: 325 FIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYKQCYIYGT 384
           F+A+D+ F+NTAGP  GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CYI GT
Sbjct: 371 FVAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGT 430

Query: 385 IDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAVDLQPI 444
           IDFIFGNAAVV QNC+I VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA DL+P+
Sbjct: 431 IDFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPV 490

Query: 445 IGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAW-QNSRFAQNTLYYGEYKNFGPRA 504
           I + KTYLGRPW+ YSR  IM++YID  +SP+GW  W + S FA NT++YGEYKNFGP +
Sbjct: 491 IRAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGS 550

Query: 505 STRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           STR+RVRW GFHAI +  VA++FTV  LIAG +WLPATGVPFK GL
Sbjct: 551 STRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of Lag0019914 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 493.0 bits (1268), Expect = 2.9e-139
Identity = 261/529 (49.34%), Postives = 349/529 (65.97%), Query Frame = 0


Query: 10  FSITLLAILSNVSSQAKSTTGNGNGINKWCKTTPHPDVCILFM--SPIKSPPENRPEFRK 69
           F I  + + S VS        N   +  WC  TP+P  C  F+  +    P ++  EF K
Sbjct: 11  FVILCILVASTVSGY------NQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLK 70

Query: 70  MAIKTAMEKAAEAQTYAARFGPSCKTTRQRAAWADCLKLYNDAVFQLNRTLQGLAGRTHG 129
           +++K  +++A  A+T+A   GP C+ TR++AAW DC+KLY+  V ++N T+         
Sbjct: 71  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD-------P 130

Query: 130 RHRCTDFDAQTWLSSALTNIDMCLSGAADLNVTEFITPIRCQKVSKMISNCLAINGRFLE 189
             +C+  DAQTWLS+ALTN+D C +G  +L VT+ + P+    VS ++ N LAIN     
Sbjct: 131 NVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAIN----- 190

Query: 190 EGVKSGAKGVENQNGFPRWVSSGDRKLLQAAKVRANLVVAKDGSGNFRSVQAAINAAARR 249
             V       E ++GFP WV  GDRKLLQ++  + N VVAKDGSGNF++++ AI+AA+  
Sbjct: 191 -KVPFNYTPPE-KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS-- 250

Query: 250 RRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI 309
              GRFVIYVK+GVY EN+E+     N+ML GDG+  T+IT  +SV  G TTF+SAT   
Sbjct: 251 -GSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAA 310

Query: 310 QGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFYKQCY 369
            G  FIAR I F NTAG    QAVALRS SDLSVF++CSF+ YQDTL V S RQFY+ C 
Sbjct: 311 VGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCD 370

Query: 370 IYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAVD 429
           +YGT+DFIFGNAA VLQNC I+ RRP + + N ITAQGR DP QN+GI IHNS++ AA D
Sbjct: 371 VYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASD 430

Query: 430 LQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEYKNFG 489
           L+P++GS KTYLGRPW++YSRTV M++ +D L+ P GWL W +  FA  TL+Y E++N G
Sbjct: 431 LRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEW-DGNFALKTLFYAEFQNTG 490

Query: 490 PRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
           P AST  RV WPGF  + + + A+KFTV   +AG +W+P++ VPF  GL
Sbjct: 491 PGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

[Close]

BLAST of Lag0019914 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 390.6 bits (1002), Expect = 2.1e-108
Identity = 232/533 (43.53%), Postives = 314/533 (58.91%), Query Frame = 0


Query: 39  CKTTPHPDVCILFM--SPIKSPPENRP--EFRKMAIKTAMEKAAEAQTYAARFGPSCKTT 98
           CK TP+P VC   M  SP+K+  +      F  + + + M++A +     +         
Sbjct: 38  CKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQHHSLH 97

Query: 99  RQ-RAAWADCLKLYNDAVFQLNRTLQGLAGRTHGRHRCTDFDAQTWLSSALTNIDMCLSG 158
           +   +A  DCL+LY D + QLN      + R++G++  +  D QT LS+A+ N D C +G
Sbjct: 98  KHATSALFDCLELYEDTIDQLNH-----SRRSYGQY-SSPHDRQTSLSAAIANQDTCRNG 157

Query: 159 AADLNVTEFIT---PIRCQK-VSKMISNCLAINGRFLE-EGV---------------KSG 218
             D  +T   +   P++  + ++K ISN LA+     E E V               +S 
Sbjct: 158 FRDFKLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSS 217

Query: 219 AKG-------VENQNGFPRWVSSGDRKLLQAAK--VRANLVVAKDGSGNFRSVQAAINAA 278
           A G       + +   FP W    DRKLL+ +K   +A+LVVAKDGSG++ S+Q A+NAA
Sbjct: 218 AGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAA 277

Query: 279 AR-RRRRGRFVIYVKRGVYRENIEVGTNNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSA 338
           A+  RR  R VIYVK GVYREN+ +  +  N+M++GDG+  T++T  R+V  G TTF SA
Sbjct: 278 AKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSA 337

Query: 339 TAGIQGPAFIARDIAFVNTAGPQMGQAVALRSSSDLSVFHRCSFQGYQDTLLVLSQRQFY 398
           T  + G  FIA+ I F NTAGP+  QAVALRSSSD SVF+ CSF+GYQDTL + S RQF 
Sbjct: 338 TFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFL 397

Query: 399 KQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIR 458
           + C IYGT+DFIFG+A  +LQNC IY R+P+ GQ N ITAQ R++P + +G  I +S + 
Sbjct: 398 RNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVA 457

Query: 459 AAVDLQPIIGSVKTYLGRPWKKYSRTVIMRSYIDKLVSPMGWLAWQNSRFAQNTLYYGEY 518
            A +         TYLGRPW+ +SRTV M+  +  LVSP GWL W  S FA +TLYYGEY
Sbjct: 458 TASE---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGS-FALSTLYYGEY 517

Query: 519 KNFGPRASTRFRVRWPGFHAIKNPNVAAKFTVRRLIAGQTWLPATGVPFKLGL 537
            N G  AS   RV+WPG+H IK    A KFTV   + G  W+ ATGVP   GL
Sbjct: 518 GNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

The following BLAST results are available for this feature:

BLAST of Lag0019914 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905752.1	1.3e-253	82.29	probable pectinesterase/pectinesterase inhibitor 60 [Benincasa hispida]	[more]
KAG7015521.1	4.9e-253	82.71	putative pectinesterase/pectinesterase inhibitor 33, partial [Cucurbita argyrosp...	[more]
XP_022932129.1	8.3e-253	82.71	probable pectinesterase/pectinesterase inhibitor 33 [Cucurbita moschata]	[more]
XP_022985199.1	7.1e-252	82.33	probable pectinesterase/pectinesterase inhibitor 33 [Cucurbita maxima]	[more]
XP_023520442.1	3.5e-251	81.95	probable pectinesterase/pectinesterase inhibitor 59 isoform X1 [Cucurbita pepo s...	[more]

BLAST of Lag0019914 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHN5	5.9e-169	56.53	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN4	2.5e-167	57.30	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	7.3e-167	56.27	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]
O22149	4.2e-138	49.34	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
O04887	1.6e-137	50.40	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]

BLAST of Lag0019914 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EW59	4.0e-253	82.71	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438452 PE=3 SV=1	[more]
A0A6J1JCM7	3.4e-252	82.33	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483275 PE=3 SV=1	[more]
A0A1S3BUR4	1.2e-241	80.11	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493398 PE=3 SV=1	[more]
A0A0A0L330	6.7e-240	78.52	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G088980 PE=3 SV=1	[more]
A0A5A7TVK4	3.2e-226	81.63	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G001080...	[more]

BLAST of Lag0019914 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51490.1	4.2e-170	56.53	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	1.8e-168	57.30	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	5.2e-168	56.27	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G45220.1	2.9e-139	49.34	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G23200.1	2.1e-108	43.53	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..181 e-value: 2.4E-32 score: 123.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 8..182 e-value: 2.1E-24 score: 83.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 35..181 e-value: 3.6E-27 score: 95.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 31..185 e-value: 8.8E-30 score: 105.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 34..185
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 223..521 e-value: 1.0E-127 score: 425.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 217..536 e-value: 1.6E-110 score: 371.4
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 8..535
None	No IPR available	PANTHER	PTHR31707:SF45	PECTINESTERASE/PECTINESTERASE INHIBITOR 59-RELATED	coord: 8..535
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 38..185 e-value: 5.19552E-37 score: 132.177
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 368..377
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 220..535

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0019914.1	Lag0019914.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity