Lag0018773 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018773
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr5: 34181440 .. 34182860 (+)
RNA-Seq ExpressionLag0018773
SyntenyLag0018773
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGATTGGAAAAGGCTCACAACCTCCCCTGGATCATTGGAGGGGATTTCAACGAAATAATGTTCAACAAGGAGAAAAAAAGGGGTTACCCTAAGCCTCTTAGATTTATCAATGATTTATGCGATTCCATTAGAATATGTAATCTTATTGACTTGGGTTTTATTGGTGACAGGTGCACTTGGGCTAAAAATAAAAGCAACCATGGAGCTACCCGAGAGAGACTTGATAGATATTTAGCTAACCCCAAAATGCTTGATATTGTCAAGGACATGAGAGTGGAGCACCTCCAATTCCACCATTCGGACCATAGGGCTATCCTGCTCAAGATTGATTGGGGAGATACTAATCAACAACAATCCTTTCATAAGAGGTTGGCCAAATTGGAACAAAGCTGGTTGCAGTATGATGGTAGTAAAAAATCCTTCGAGGACGAATGGAAATCATGGTCGTGTATATCCAATTTTAACTTCAATAGTCGAGTTTAGCAAGGTTTAGAGGCCATGAAACAGTGGAATAAAGAGAGATTGAAAGGGTCCTTAAAAGGGGCATTACCCGAAAGGAGCTCGAAATCAAGAGACTGTCCAATCCTAAAGACCAAGCCGCGCTGGACCTTCTATCCAAAGCCGAATGGGAGCTGGAAAAGCTCCTGGAAGAAGAAGAAAGTTATTGGCACATGAGAGCTAGAGAGGAGTGGCTTAAGAGTGGAGACAAGAACACAAAGTGGTTCCACTCAAAAGCCACTCAAAGAAAGAAAAGAAACGAGATAAAAGGCATCTTTAACAGTAGAGGAATCTGGGTCGAAGATATCAAAGAGATAGGGACAGCGACTACTGAATACTATAACTCCCTTCTAACATCGTCACATCCAAACAGAGAAGACACCAAGGAAGTCACCAAGGCTATTACTAAAAAGATTTCTGAGGATCAAAAGGCCAAGCTAGATGCCTCTTTTTCCAGAGAAGAAGTCGAGAAAGCTATTAAAGGAATGAGTCCTTCCAAAGCCCCGGGTCCTGACGGGGCGCACGCTATGCTTTTCCAAAATTTGTGGGAGACCATGGGGGAGGATACAGTGAAAACATGCTTGGGGATCCTAAACAACAAGGAAGAGATTGAGCCTATAAATAAAACTCTTATAGCGCTTATCCCTAAAACCAAGGACCCAAAGACCATGAATGAGTTCAGACCAATTAGCCTGTGCAATGTGATTTACAAAGTGATAGCTAAAGCCCTAGCGAATCGCATGAAGCAGGTGCTCGACACGATCATTTCCCCTTCCCAATCGGCATTCATTCCTGGAAGACAAATCACTGATAACGTTCTGGTGGGGTTCGAATGTATCCATGTGTTGAATAATAGAAGAACTGGAAGAACCGGATATGTGGCTATCAAACTCGACATGAGTAAAGCCTATGACTGA

mRNA sequence

ATGGAAAGATTGGAAAAGGCTCACAACCTCCCCTGGATCATTGGAGGGGATTTCAACGAAATAATGTTCAACAAGGAGAAAAAAAGGGGTTACCCTAAGCCTCTTAGATTTATCAATGATTTATGCGATTCCATTAGAATATGTAATCTTATTGACTTGGGTTTTATTGGTGACAGGTGCACTTGGGCTAAAAATAAAAGCAACCATGGAGCTACCCGAGAGAGACTTGATAGATATTTAGCTAACCCCAAAATGCTTGATATTGTCAAGGACATGAGAGTGGAGCACCTCCAATTCCACCATTCGGACCATAGGGCTATCCTGCTCAAGATTGATTGGGGAGATACTAATCAACAACAATCCTTTCATAAGAGGTTGGCCAAATTGGAACAAAGCTGGTTGCAGGGCATTACCCGAAAGGAGCTCGAAATCAAGAGACTGTCCAATCCTAAAGACCAAGCCGCGCTGGACCTTCTATCCAAAGCCGAATGGGAGCTGGAAAAGCTCCTGGAAGAAGAAGAAAGTTATTGGCACATGAGAGCTAGAGAGGAGTGGCTTAAGAGTGGAGACAAGAACACAAAGTGGTTCCACTCAAAAGCCACTCAAAGAAAGAAAAGAAACGAGATAAAAGGCATCTTTAACAGTAGAGGAATCTGGGTCGAAGATATCAAAGAGATAGGGACAGCGACTACTGAATACTATAACTCCCTTCTAACATCGTCACATCCAAACAGAGAAGACACCAAGGAAGTCACCAAGGCTATTACTAAAAAGATTTCTGAGGATCAAAAGGCCAAGCTAGATGCCTCTTTTTCCAGAGAAGAAGTCGAGAAAGCTATTAAAGGAATGAGTCCTTCCAAAGCCCCGGGTCCTGACGGGGCGCACGCTATGCTTTTCCAAAATTTGTGGGAGACCATGGGGGAGGATACAGTGAAAACATGCTTGGGGATCCTAAACAACAAGGAAGAGATTGAGCCTATAAATAAAACTCTTATAGCGCTTATCCCTAAAACCAAGGACCCAAAGACCATGAATGAGTTCAGACCAATTAGCCTGTGCAATGTGATTTACAAAGTGATAGCTAAAGCCCTAGCGAATCGCATGAAGCAGGTGCTCGACACGATCATTTCCCCTTCCCAATCGGCATTCATTCCTGGAAGACAAATCACTGATAACGTTCTGGTGGGGTTCGAATGTATCCATGTGTTGAATAATAGAAGAACTGGAAGAACCGGATATGTGGCTATCAAACTCGACATGAGTAAAGCCTATGACTGA

Coding sequence (CDS)

ATGGAAAGATTGGAAAAGGCTCACAACCTCCCCTGGATCATTGGAGGGGATTTCAACGAAATAATGTTCAACAAGGAGAAAAAAAGGGGTTACCCTAAGCCTCTTAGATTTATCAATGATTTATGCGATTCCATTAGAATATGTAATCTTATTGACTTGGGTTTTATTGGTGACAGGTGCACTTGGGCTAAAAATAAAAGCAACCATGGAGCTACCCGAGAGAGACTTGATAGATATTTAGCTAACCCCAAAATGCTTGATATTGTCAAGGACATGAGAGTGGAGCACCTCCAATTCCACCATTCGGACCATAGGGCTATCCTGCTCAAGATTGATTGGGGAGATACTAATCAACAACAATCCTTTCATAAGAGGTTGGCCAAATTGGAACAAAGCTGGTTGCAGGGCATTACCCGAAAGGAGCTCGAAATCAAGAGACTGTCCAATCCTAAAGACCAAGCCGCGCTGGACCTTCTATCCAAAGCCGAATGGGAGCTGGAAAAGCTCCTGGAAGAAGAAGAAAGTTATTGGCACATGAGAGCTAGAGAGGAGTGGCTTAAGAGTGGAGACAAGAACACAAAGTGGTTCCACTCAAAAGCCACTCAAAGAAAGAAAAGAAACGAGATAAAAGGCATCTTTAACAGTAGAGGAATCTGGGTCGAAGATATCAAAGAGATAGGGACAGCGACTACTGAATACTATAACTCCCTTCTAACATCGTCACATCCAAACAGAGAAGACACCAAGGAAGTCACCAAGGCTATTACTAAAAAGATTTCTGAGGATCAAAAGGCCAAGCTAGATGCCTCTTTTTCCAGAGAAGAAGTCGAGAAAGCTATTAAAGGAATGAGTCCTTCCAAAGCCCCGGGTCCTGACGGGGCGCACGCTATGCTTTTCCAAAATTTGTGGGAGACCATGGGGGAGGATACAGTGAAAACATGCTTGGGGATCCTAAACAACAAGGAAGAGATTGAGCCTATAAATAAAACTCTTATAGCGCTTATCCCTAAAACCAAGGACCCAAAGACCATGAATGAGTTCAGACCAATTAGCCTGTGCAATGTGATTTACAAAGTGATAGCTAAAGCCCTAGCGAATCGCATGAAGCAGGTGCTCGACACGATCATTTCCCCTTCCCAATCGGCATTCATTCCTGGAAGACAAATCACTGATAACGTTCTGGTGGGGTTCGAATGTATCCATGTGTTGAATAATAGAAGAACTGGAAGAACCGGATATGTGGCTATCAAACTCGACATGAGTAAAGCCTATGACTGA

Protein sequence

MERLEKAHNLPWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRCTWAKNKSNHGATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQSFHKRLAKLEQSWLQGITRKELEIKRLSNPKDQAALDLLSKAEWELEKLLEEEESYWHMRAREEWLKSGDKNTKWFHSKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNREDTKEVTKAITKKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGEDTVKTCLGILNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALANRMKQVLDTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKAYD
Homology
BLAST of Lag0018773 vs. NCBI nr
Match: XP_030923330.1 (uncharacterized protein LOC115950239 [Quercus lobata])

HSP 1 Score: 317.0 bits (811), Expect = 2.5e-82
Identity = 183/465 (39.35%), Postives = 267/465 (57.42%), Query Frame = 0

Query: 11  PWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRCTWAKNKSNHG 70
           PW++ GDFN  +   EK          I    +++  C L DLGF G   TW+  +    
Sbjct: 523 PWVVIGDFNAYLHASEKMSARQPQFSQIEAFREALSSCQLHDLGFKGYPYTWSNKRPGEA 582

Query: 71  ATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQSFHKRLAKLE 130
            T+ RLDR +AN +  D  +  RV HL  H SDH  +LL +     +Q +    R  K E
Sbjct: 583 NTKIRLDRGVANKEWTDRFQMSRVVHLSSHASDHLPLLLHVQ--SFSQPRQHCGRSFKFE 642

Query: 131 QSWL--------------------QGITRKELEIK-----------RLSNP------KDQ 190
           +SWL                     G+   + +IK            +++P      + Q
Sbjct: 643 ESWLLQDECAAVIQEAWGNGDGNRDGLAAVQEKIKACGVELMAWGSSITDPDTGAIKEIQ 702

Query: 191 AALDLL--------SKAEW-----ELEKLLEEEESYWHMRAREEWLKSGDKNTKWFHSKA 250
             LD L        SKAE+     +++ LL+++E YW  R+R  WL+ GD+NTK+FH+KA
Sbjct: 703 KQLDRLNETELTEASKAEFLNLSKKMDDLLQKQEIYWAQRSRINWLRHGDRNTKFFHAKA 762

Query: 251 TQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNREDTKEVTKAITKKIS 310
           +QR+++N I+GI NS+G WVE+++E+G    +Y+++L  +     +  +E   A+  K++
Sbjct: 763 SQRRRKNFIRGIRNSQGQWVENLEEVGQVAADYFDNLFQAG--AGDQMEECLDAVDTKVT 822

Query: 311 EDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGEDTVKTCLGILNN 370
           ED +  L   F+ EEV+ A+  M P+KAPGPDG +A+ +Q  W  +G+  V   L  LNN
Sbjct: 823 EDMREFLSNQFTAEEVQAALFQMGPTKAPGPDGMNALFYQKFWHIVGDSVVSAVLDFLNN 882

Query: 371 KEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALANRMKQVLDTIISPSQ 426
              +  IN T I LIPK ++P+ M+EFRPISLCNVIYK+I+K LANR+KQVL  IIS +Q
Sbjct: 883 GNMLPEINHTNIVLIPKVQNPERMSEFRPISLCNVIYKIISKVLANRLKQVLPQIISSTQ 942

BLAST of Lag0018773 vs. NCBI nr
Match: PRQ56718.1 (putative RNA-directed DNA polymerase [Rosa chinensis])

HSP 1 Score: 315.5 bits (807), Expect = 7.2e-82
Identity = 179/480 (37.29%), Postives = 258/480 (53.75%), Query Frame = 0

Query: 1   MERLEKAHNLPWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRC 60
           +++L    NLPW++GGD+NEI    +K  G  + +R +ND+ +++  C L D+ F+G R 
Sbjct: 132 LKQLSMHGNLPWVVGGDYNEICERSDKMGGVLRSVRLMNDMKEALVFCELDDIQFVGPRF 191

Query: 61  TWAKNKSNHGATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQ 120
           TW   +      R RLDR+  +    D+    RV HL    SDH  ILL++      +++
Sbjct: 192 TWRGIRGGE-EVRVRLDRFTCSLPWRDLFPASRVRHLDPSTSDHVPILLEVRIQKKRRKK 251

Query: 121 SFHKRLAKLEQSWLQGITRKEL-------------------------------------- 180
              KR  K E+ WL   T KE+                                      
Sbjct: 252 RKKKRF-KFEECWLLDETCKEVVKSSWESVSGVGPTQLLWNRMQSTRAALESWSEKSFGC 311

Query: 181 ---EIKRLSN--------------PKDQAALDLLSKAEWELEKLLEEEESYWHMRAREEW 240
              EI ++ N               +D+ AL L      +L  LL +E+ +W  RA+  W
Sbjct: 312 IRKEIAKIRNQLSVFYDSSLSAPLEEDRVALQL------KLNDLLHQEQVFWRQRAKVFW 371

Query: 241 LKSGDKNTKWFHSKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNR 300
           LK GD NTK+FH +   RKK+N + G+FN+ G+W  +++E+      Y+N L TSS+P  
Sbjct: 372 LKDGDLNTKFFHQRVKNRKKKNTLTGLFNNDGVWSTEVEELEDIVLHYFNDLFTSSYP-- 431

Query: 301 EDTKEVTKAITKKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWET 360
           ED + + + +   +SE     L     +EEV  AIK M PSK+PGPDG     FQ  WE 
Sbjct: 432 EDFQNILEGVLHGVSEVDNRALTRRIDKEEVFAAIKNMHPSKSPGPDGFSQCFFQTFWEL 491

Query: 361 MGEDTVKTCLGILNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALA 420
           +G+D V        +KE +  +N T +ALIPK + P+ M++ RPISLCNV+YK+ +K LA
Sbjct: 492 VGDDIVGAVREFFVSKESLRAVNSTYVALIPKVEVPQYMHQLRPISLCNVVYKIGSKVLA 551

Query: 421 NRMKQVLDTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKAYD 426
           NR+K +LD++ISP QSAF+PGR I+DN L+ FE  H L  RR+G+ GY A+KLDMSKAYD
Sbjct: 552 NRLKPLLDSLISPFQSAFVPGRLISDNSLMAFEVSHCLKRRRSGKVGYCALKLDMSKAYD 601

BLAST of Lag0018773 vs. NCBI nr
Match: CCA66036.1 (hypothetical protein [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 313.5 bits (802), Expect = 2.7e-81
Identity = 175/468 (37.39%), Postives = 255/468 (54.49%), Query Frame = 0

Query: 1   MERLEKAHNLPWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRC 60
           +  L +A   PW+ GGDFN ++   EKK G     R  +   +++  C+ +DLGF+G   
Sbjct: 126 LSALARASRRPWLCGGDFNLMLVASEKKGGDGFNSREADIFRNAMEECHFMDLGFVGYEF 185

Query: 61  TWAKNKSNHGATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQ 120
           TW  N+      +ERLDR++AN           V HL    SDH  I+  +    +   +
Sbjct: 186 TWTNNRGGDANIQERLDRFVANDLWKIKFPGSFVSHLPKRKSDHVPIVASVKGAQSAATR 245

Query: 121 SFHKRLAKLEQSWLQ------------------GITRKELEIKRLSNPKD---------- 180
           +   +  + E  WL+                  GI       K LS  K           
Sbjct: 246 TKKSKRFRFEAMWLREGESDEVVKETWMRGTDAGINLARTANKLLSWSKQKFGHVAKEIR 305

Query: 181 --QAALDLLSKAE-------------WELEKLLEEEESYWHMRAREEWLKSGDKNTKWFH 240
             Q  + +L ++E               +++L + EE YWH R+R++W+KSGDKNTK+FH
Sbjct: 306 MCQHQMKVLMESEPSEDNIMHMRALDARMDELEKREEVYWHQRSRQDWIKSGDKNTKFFH 365

Query: 241 SKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNREDTKEVTKAITK 300
            KA+ R++RN ++ I N  G W ED  ++      Y+ +L  S   N  +   +   +  
Sbjct: 366 QKASHREQRNNVRRIRNEAGEWFEDEDDVTECFAHYFENLFQSG--NNCEMDPILNIVKP 425

Query: 301 KISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGEDTVKTCLGI 360
           +I+++   +LDA F REEV  A+  M P+KAPGPDG +A+ +Q+ W+T+GED     L +
Sbjct: 426 QITDELGTQLDAPFRREEVSAALAQMHPNKAPGPDGMNALFYQHFWDTIGEDVTTKVLNM 485

Query: 361 LNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALANRMKQVLDTIIS 420
           LNN + I  +N+T I LIPK K  ++  +FRPISLCNV+YK++AK LANRMK VL  +I 
Sbjct: 486 LNNVDNIGAVNQTHIVLIPKKKHCESPVDFRPISLCNVLYKIVAKVLANRMKMVLPMVIH 545

Query: 421 PSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKAYD 426
            SQS F+PGR ITDNVLV +EC H L  ++TG+ GY+ +KLDMSKAYD
Sbjct: 546 ESQSGFVPGRLITDNVLVAYECFHFLRKKKTGKKGYLGLKLDMSKAYD 591

BLAST of Lag0018773 vs. NCBI nr
Match: XP_010673168.1 (PREDICTED: uncharacterized protein LOC104889608 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 312.8 bits (800), Expect = 4.7e-81
Identity = 175/459 (38.13%), Postives = 256/459 (55.77%), Query Frame = 0

Query: 11  PWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRCTWAKNKSNHG 70
           P + GGDFNE++   E + G     R ++D  + +   +L DLGF G   TW + K+   
Sbjct: 512 PVLFGGDFNEVLSMSEVEGGRVSDRRAMHDFREVVDELHLRDLGFSGLWYTWERGKNPTT 571

Query: 71  ATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQSFHKRLAKLE 130
             RERLDR+LA+P+  D    + VEH+  + SDH  I++++ +G   +++   K+  +  
Sbjct: 572 QIRERLDRFLASPQWFDCFPQVHVEHMVRYKSDHTPIMVQL-FGCKRRRKKRKKKRFRFG 631

Query: 131 QSWL--------------------------------------------QGITRKELEIKR 190
            +WL                                            + I   E EIKR
Sbjct: 632 TAWLLEDSCESLVRTAWDHSSGLPFEARIGAVAQDLVVWSKDTLNHLGREICLVEEEIKR 691

Query: 191 LSNPKDQAALDLLSKAEWELEKLLEEEESYWHMRAREEWLKSGDKNTKWFHSKATQRKKR 250
           L +    A  + L +   +L+ LLE++E+YW++R+R   +K GDKNTK+FH KA+QRK+R
Sbjct: 692 LQHSSIAADQEHLMECHSKLDGLLEKQEAYWYLRSRVAEIKDGDKNTKYFHHKASQRKRR 751

Query: 251 NEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNREDTKEVTKAITKKISEDQKAK 310
           N I G+F+   +W +D ++I      YY +L TSS P+ E    V  A+   ISE+    
Sbjct: 752 NYINGLFDEGDVWRDDDEDIERVVEAYYKNLFTSSLPSDEALSAVLDAVVPIISEEMNVV 811

Query: 311 LDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGEDTVKTCLGILNNKEEIEP 370
           L     +EEV +A++ M PSKAPGPDG HA+ +Q  W  +G+D      GI++     + 
Sbjct: 812 LCRRVVKEEVWEALRQMHPSKAPGPDGMHAVFYQRFWHIVGDDVTSVVAGIIHGTRPPDA 871

Query: 371 INKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALANRMKQVLDTIISPSQSAFIPG 426
           +N T IALIPK K P  ++EFRPISLCNVI+K++ K LANR+K +L  ++S +QSAF+PG
Sbjct: 872 LNNTNIALIPKVKSPTLVSEFRPISLCNVIFKLVTKVLANRLKTILPGVVSENQSAFVPG 931

BLAST of Lag0018773 vs. NCBI nr
Match: XP_023908235.1 (uncharacterized protein LOC112019924 [Quercus suber])

HSP 1 Score: 309.7 bits (792), Expect = 3.9e-80
Identity = 184/476 (38.66%), Postives = 267/476 (56.09%), Query Frame = 0

Query: 1   MERLEKAHNLPWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRC 60
           +E L    N  W+  GDFN I+ + EK    P     I+   + +  C+L DLG+ G   
Sbjct: 409 LEHLRGFVNGAWLCAGDFNAILNSAEKLSLRPPKSAEIDAFRNVLDSCSLEDLGYRGYTY 468

Query: 61  TWAKNKSNHGATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQ 120
           TW+  +     T+ RLDR +A  +         V HL  H SDH  IL+ +      QQ+
Sbjct: 469 TWSNKRPGAANTKLRLDRAVATMEWRAKFSITTVAHLPPHASDHLPILVHVKSVQRFQQK 528

Query: 121 SFHKRLAKLEQSWLQ--------------------GITRKELEIK-----------RLSN 180
              K+  K E++WL                     G+   + +IK             S 
Sbjct: 529 --FKKGFKFEEAWLMWEDCGEVVKEAWDLVGGGELGLNSIKEKIKFCGEELQAWGSSKSE 588

Query: 181 PKD------QAALDLLSKAE--------------WELEKLLEEEESYWHMRAREEWLKSG 240
           P +      Q  L++L+ AE               +L+ LL ++E YW  R+R  WLK G
Sbjct: 589 PNEVAIKTLQNRLEVLNSAEVVTEESNAEYLSVTKQLDDLLLKQEIYWAQRSRIPWLKHG 648

Query: 241 DKNTKWFHSKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNREDTK 300
           DKNTK+FHSKA+QR++RN IKGI +++G WVE+++EI    T Y+++L  +   N +  +
Sbjct: 649 DKNTKYFHSKASQRRRRNFIKGIRSAQGNWVEEMEEIANVATGYFDNLFCAG--NCDQME 708

Query: 301 EVTKAITKKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGED 360
           E   A+ +K++ +    L + F+ EEV+ A+  M P+KAPGPDG +A+ +Q  W  +G+ 
Sbjct: 709 ECLNAVPRKVTPEMHDTLSSEFNAEEVKLALFQMGPTKAPGPDGMNALFYQKFWHVVGDT 768

Query: 361 TVKTCLGILNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALANRMK 420
            V   L  LNN      IN T I LIPK K+P+ M++FRPISLCNVIYK+I+K L NR+K
Sbjct: 769 VVDAVLDFLNNGHMAPDINHTYIVLIPKVKNPEKMSDFRPISLCNVIYKIISKVLVNRLK 828

Query: 421 QVLDTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKAYD 426
           QVL  IISP+QSAF+PGR ITDNVL+ +E +H +++R+ G+ GY+A+KLD+SKAYD
Sbjct: 829 QVLPNIISPTQSAFVPGRLITDNVLLAYETLHTMHSRKKGKKGYMALKLDVSKAYD 880

BLAST of Lag0018773 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.2e-25
Identity = 85/307 (27.69%), Postives = 156/307 (50.81%), Query Frame = 0

Query: 120 QSFHKRLAKLEQSWLQGITRKELEI-KRLSNPKDQAALDLLSKAEWELEKLLEEEESYWH 179
           Q + K ++    + ++ +  + L++ +RLS  +DQA      + +  L  + + +     
Sbjct: 298 QEYTKSVSGQRNAEIEALNGEVLDLEQRLSGSEDQALQCEYLERKEALRNMEQRQARGAF 357

Query: 180 MRAREEWLKSGDKNTKWFHSKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLL 239
           +R+R + L   D+ +++F++   ++  R +I  +F   G  +ED + I      +Y +L 
Sbjct: 358 VRSRMQLLCDMDRGSRFFYALEKKKGNRKQITCLFAEDGTPLEDPEAIRDRARSFYQNLF 417

Query: 240 TSSHPNREDTKEVTKAITKKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAML 299
            S  P   D  E        +SE +K +L+   + +E+ +A++ M  +K+PG DG     
Sbjct: 418 -SPDPISPDACEELWDGLPVVSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEF 477

Query: 300 FQNLWETMGEDTVKTCLGILNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYK 359
           FQ  W+T+G D  +         E      + +++L+PK  D + +  +RP+SL +  YK
Sbjct: 478 FQFFWDTLGPDFHRVLTEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYK 537

Query: 360 VIAKALANRMKQVLDTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKL 419
           ++AKA++ R+K VL  +I P QS  +PGR I DNV +  + +H    RRTG      + L
Sbjct: 538 IVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHFA--RRTG-LSLAFLSL 597

Query: 420 DMSKAYD 426
           D  KA+D
Sbjct: 598 DQEKAFD 600

BLAST of Lag0018773 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 5.3e-19
Identity = 86/293 (29.35%), Postives = 137/293 (46.76%), Query Frame = 0

Query: 140 KELEIKRLSNPKDQAALDLLSKAEWELEKLLEEEESYWHMRAREEWLKSGDKNTKWFHSK 199
           K LE K  ++PK     +++ K   E+ ++          + R  + +  +K  K     
Sbjct: 329 KALEKKEANSPKRSRRQEII-KLRGEINQVETRRTIQRINQTRSWFFEKINKIDKPLARL 388

Query: 200 ATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNRED-TKEVTKAITKK 259
               + +  I  I N +G    D +EI      +Y  L ++   N ++  K + +    K
Sbjct: 389 TKGHRDKILINKIRNEKGDITTDPEEIQNTIRSFYKRLYSTKLENLDEMDKFLDRYQVPK 448

Query: 260 ISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGEDTVKTCLGIL 319
           +++DQ   L++  S +E+E  I  +   K+PGPDG  A  +Q    T  ED +   L  L
Sbjct: 449 LNQDQVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQ----TFKEDLI-PILHKL 508

Query: 320 NNKEEIE-----PINKTLIALIPK-TKDPKTMNEFRPISLCNVIYKVIAKALANRMKQVL 379
            +K E+E        +  I LIPK  KDP  +  FRPISL N+  K++ K LANR+++ +
Sbjct: 509 FHKIEVEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHI 568

Query: 380 DTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKAYD 426
             II P Q  FIPG Q   N+      IH +N  +     ++ I LD  KA+D
Sbjct: 569 KAIIHPDQVGFIPGMQGWFNIRKSINVIHYINKLKD--KNHMIISLDAEKAFD 613

BLAST of Lag0018773 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.3e-17
Identity = 86/312 (27.56%), Postives = 145/312 (46.47%), Query Frame = 0

Query: 120 QSFHKRLAKLEQSWLQGITRKELEIKRLSNPKDQAALDLLSKAEWELEKLLEEEESYWHM 179
           Q+F K+  + E + L G   K+LE +  SNPK     + ++K   EL ++  +       
Sbjct: 302 QAFLKKTEREEVNNLMG-HLKQLEKEEHSNPKPSRRKE-ITKIRAELNEIENKRIIQQIN 361

Query: 180 RAREEWLKSGDKNTKWFHSKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLT 239
           +++  + +  +K  K   +   +++ ++ I  I N       D  EI     EYY  L +
Sbjct: 362 KSKSWFFEKINKIDKPLANLTRKKRVKSLISSIRNGNDEITTDPSEIQKILNEYYKKLYS 421

Query: 240 SSHPNREDTKEVTKAI-TKKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAML 299
             + N ++  +  +A    ++S+ +   L+   S  E+   I+ +   K+PGPDG  +  
Sbjct: 422 HKYENLKEIDQYLEACHLPRLSQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEF 481

Query: 300 FQNLWETMGEDTVKTCLGILNN--KEEIEP--INKTLIALIPKT-KDPKTMNEFRPISLC 359
           +Q    T  E+ V   L +  N  KE I P    +  I LIPK  KDP     +RPISL 
Sbjct: 482 YQ----TFKEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGKDPTRKENYRPISLM 541

Query: 360 NVIYKVIAKALANRMKQVLDTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGY 419
           N+  K++ K L NR++Q +  II   Q  FIPG Q   N+      I  +N  +     +
Sbjct: 542 NIDAKILNKILTNRIQQHIKKIIHHDQVGFIPGSQGWFNIRKSINVIQHINKLK--NKDH 601

Query: 420 VAIKLDMSKAYD 426
           + + +D  KA+D
Sbjct: 602 MILSIDAEKAFD 605

BLAST of Lag0018773 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.4e-16
Identity = 85/304 (27.96%), Postives = 141/304 (46.38%), Query Frame = 0

Query: 128 KLEQSWLQGITR--KELEIKRLSNPKDQAALDLLSKAEWELEKLLEEEESYWHMRAREEW 187
           K E+S +  +T   KELE +  ++ K     + ++K   EL+++  ++       +R  +
Sbjct: 308 KQERSKIDTLTSQLKELEKQEQTHSKASRRQE-ITKIRAELKEIETQKTLQKINESRSWF 367

Query: 188 LKSGDKNTKWFHSKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNR 247
            +  +K  +       +++++N+I  I N +G    D  EI T   EYY  L  +   N 
Sbjct: 368 FERINKIDRPLARLIKKKREKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANKLENL 427

Query: 248 EDTKEVTKAIT-KKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWE 307
           E+        T  ++++++   L+   +  E+   I  +   K+PGPDG  A  +Q   E
Sbjct: 428 EEMDTFLDTYTLPRLNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKE 487

Query: 308 TMGEDTVKTCLGILNNKEEIEP--INKTLIALIPKT-KDPKTMNEFRPISLCNVIYKVIA 367
            +    +K    I   KE I P    +  I LIPK  +D      FRPISL N+  K++ 
Sbjct: 488 ELVPFLLKLFQSI--EKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILN 547

Query: 368 KALANRMKQVLDTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMS 426
           K LANR++Q +  +I   Q  FIPG Q   N+      I  +N  R     +V I +D  
Sbjct: 548 KILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQHIN--RAKDKNHVIISIDAE 606

BLAST of Lag0018773 vs. ExPASy Swiss-Prot
Match: Q03274 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 2.5e-05
Identity = 45/157 (28.66%), Postives = 81/157 (51.59%), Query Frame = 0

Query: 272 SREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWET-MGEDTVKTCLGILNNKEEIEPINKT 331
           +REE++ AIKG  PS APG DG   +  Q +  T +  + V+  L +L       P    
Sbjct: 7   AREEIQCAIKGWKPS-APGSDG---LTVQAITRTRLPRNFVQ--LHLLRGHVP-TPWTAM 66

Query: 332 LIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALANRMKQVLDTIISPSQSAF--IPGRQ 391
              LIPK  D +  + +RPI++ + + +++ + LA R++  ++  + P+Q  +  I G  
Sbjct: 67  RTTLIPKDGDLENPSNWRPITIASALQRLLHRILAKRLEAAVE--LHPAQKGYARIDGTL 126

Query: 392 ITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKAYD 426
           +   +L  +     +++RR  R  Y  + LD+ KA+D
Sbjct: 127 VNSLLLDTY-----ISSRREQRKTYNVVSLDVRKAFD 149

BLAST of Lag0018773 vs. ExPASy TrEMBL
Match: A0A2N9EX83 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7086 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 4.1e-83
Identity = 171/422 (40.52%), Postives = 245/422 (58.06%), Query Frame = 0

Query: 4   LEKAHNLPWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRCTWA 63
           L   H LPW  GGDFNE++  +EK     +P   + +    +  C  +DLGF+G   TW 
Sbjct: 496 LRSHHTLPWFCGGDFNELLQAEEKWGRVARPEHQMREFRRVVDDCGFVDLGFVGSPYTWW 555

Query: 64  KNKSNHGATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQSFH 123
             ++      ERLDR LA    L    + RV HLQ   SDH+ + +++ W   N +    
Sbjct: 556 NKQTGTARVLERLDRCLATADWLLKFPNCRVTHLQAVFSDHQPLWVELHWSGRNLRPC-- 615

Query: 124 KRLAKLEQSWLQGITRKELEIKRLSNPKDQAALDLLSKAEWELEKLLEEEESYWHMRARE 183
           ++  + E+ W    +  E  IK+    +   +L +  K   EL  L  +EE  W  R+R 
Sbjct: 616 RKRFRFEEMWTLH-SGCEDTIKKAWESRQSGSL-MFQKLNGELADLHAKEERMWKQRSRS 675

Query: 184 EWLKSGDKNTKWFHSKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHP 243
            WL+SGD+NTK+FH +AT RK+RN I GI +  G+W    +E+     EYY  L T+S P
Sbjct: 676 LWLQSGDRNTKFFHCQATYRKRRNHIHGIRDRVGVWQSGAEEVEHTIVEYYKDLFTTSQP 735

Query: 244 NREDTKEVTKAITKKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLW 303
              D  E+   + + I+ D   +LDA F+  EVE A+  M P KAPGPDG   + +Q  W
Sbjct: 736 GNFD--EILSGVDRMITIDMNQQLDAEFTAAEVEHALNQMGPLKAPGPDGMAPIFYQKYW 795

Query: 304 ETMGEDTVKTCLGILNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKA 363
             +G D   + L  L +   ++ IN T I LIPK ++P+++ +FRPISLCNVIYK+IAK 
Sbjct: 796 NIVGHDVTASILSCLRDGSLLKKINHTHICLIPKVQNPESVKDFRPISLCNVIYKIIAKV 855

Query: 364 LANRMKQVLDTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKA 423
           LANR+K++L  IIS SQSAF+PGR I+DN+L+ FE +H + + +  + GY+A+KLDMSKA
Sbjct: 856 LANRLKKILPHIISESQSAFVPGRLISDNILIAFETLHHMKHMKGNKQGYMALKLDMSKA 911

Query: 424 YD 426
           YD
Sbjct: 916 YD 911

BLAST of Lag0018773 vs. ExPASy TrEMBL
Match: A0A803NML1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 7.0e-83
Identity = 173/465 (37.20%), Postives = 258/465 (55.48%), Query Frame = 0

Query: 10   LPWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRCTWAKNKSNH 69
            LPW++ GDFNEI+ N +K  G  +  + ++   D++  C+L    F GD+ TW K + N 
Sbjct: 575  LPWMVIGDFNEILSNHDKFGGGLRNEQQMDAFRDTLSFCSLHQQPFHGDKFTWIKGRHNP 634

Query: 70   GATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQSFHKRLAKL 129
             A +ERLD    N     I K +   HL ++ SDHRAI + ID+  +NQQQ   K   + 
Sbjct: 635  NALKERLDWCFLNDLWETIFKPITTAHLDYYSSDHRAIAVTIDYITSNQQQLTRKTRFRF 694

Query: 130  EQSWL-----------------------------------------------QGITRKEL 189
            E+ WL                                               + IT  + 
Sbjct: 695  EKIWLKEPDAAAIIRAHWSDTDAGGMDIFLANLQSCTDALQQWHIRKFGNMKKNITNMQQ 754

Query: 190  EIKRLSNPKDQ--AALDLLSKAEWELEKLLEEEESYWHMRAREEWLKSGDKNTKWFHSKA 249
            ++ RL+N  D+  + +D L  +E  L++LL++EE+YWH R+R + L+ GD+NT +FH+ A
Sbjct: 755  QVSRLNNAADRSVSTMDELKDSEAILDELLQQEETYWHQRSRVDSLQCGDQNTSFFHAHA 814

Query: 250  TQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNREDTKEVTKAITKKIS 309
            T RK +N IK + N++G  V    E+    ++YY SL  S   + +   ++   +   I+
Sbjct: 815  TSRKSKNVIKSLINTQGFSVSSKAEMTNVISDYYTSLFASDGVDPDSLNDILTTVPTTIT 874

Query: 310  EDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGEDTVKTCLGILNN 369
             +    L   F+  EV +A++ MSP K+PG DG  AM +Q+ W  +G       LG+LN+
Sbjct: 875  HEMNQSLTTPFTSNEVYEALQSMSPDKSPGCDGMSAMFYQHYWNIVGSSVTSLVLGVLND 934

Query: 370  KEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALANRMKQVLDTIISPSQ 426
               +  +NK++I LIPK  +P +M E+RPISLCNVIYK+I+K + NR KQVL  +IS +Q
Sbjct: 935  GNNLTDLNKSIITLIPKVANPTSMTEYRPISLCNVIYKLISKTIVNRFKQVLPFVISETQ 994

BLAST of Lag0018773 vs. ExPASy TrEMBL
Match: A0A2P6SDG4 (Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0340391 PE=4 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 3.5e-82
Identity = 179/480 (37.29%), Postives = 258/480 (53.75%), Query Frame = 0

Query: 1   MERLEKAHNLPWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRC 60
           +++L    NLPW++GGD+NEI    +K  G  + +R +ND+ +++  C L D+ F+G R 
Sbjct: 132 LKQLSMHGNLPWVVGGDYNEICERSDKMGGVLRSVRLMNDMKEALVFCELDDIQFVGPRF 191

Query: 61  TWAKNKSNHGATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQ 120
           TW   +      R RLDR+  +    D+    RV HL    SDH  ILL++      +++
Sbjct: 192 TWRGIRGGE-EVRVRLDRFTCSLPWRDLFPASRVRHLDPSTSDHVPILLEVRIQKKRRKK 251

Query: 121 SFHKRLAKLEQSWLQGITRKEL-------------------------------------- 180
              KR  K E+ WL   T KE+                                      
Sbjct: 252 RKKKRF-KFEECWLLDETCKEVVKSSWESVSGVGPTQLLWNRMQSTRAALESWSEKSFGC 311

Query: 181 ---EIKRLSN--------------PKDQAALDLLSKAEWELEKLLEEEESYWHMRAREEW 240
              EI ++ N               +D+ AL L      +L  LL +E+ +W  RA+  W
Sbjct: 312 IRKEIAKIRNQLSVFYDSSLSAPLEEDRVALQL------KLNDLLHQEQVFWRQRAKVFW 371

Query: 241 LKSGDKNTKWFHSKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNR 300
           LK GD NTK+FH +   RKK+N + G+FN+ G+W  +++E+      Y+N L TSS+P  
Sbjct: 372 LKDGDLNTKFFHQRVKNRKKKNTLTGLFNNDGVWSTEVEELEDIVLHYFNDLFTSSYP-- 431

Query: 301 EDTKEVTKAITKKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWET 360
           ED + + + +   +SE     L     +EEV  AIK M PSK+PGPDG     FQ  WE 
Sbjct: 432 EDFQNILEGVLHGVSEVDNRALTRRIDKEEVFAAIKNMHPSKSPGPDGFSQCFFQTFWEL 491

Query: 361 MGEDTVKTCLGILNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALA 420
           +G+D V        +KE +  +N T +ALIPK + P+ M++ RPISLCNV+YK+ +K LA
Sbjct: 492 VGDDIVGAVREFFVSKESLRAVNSTYVALIPKVEVPQYMHQLRPISLCNVVYKIGSKVLA 551

Query: 421 NRMKQVLDTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKAYD 426
           NR+K +LD++ISP QSAF+PGR I+DN L+ FE  H L  RR+G+ GY A+KLDMSKAYD
Sbjct: 552 NRLKPLLDSLISPFQSAFVPGRLISDNSLMAFEVSHCLKRRRSGKVGYCALKLDMSKAYD 601

BLAST of Lag0018773 vs. ExPASy TrEMBL
Match: A0A2N9HE46 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS37832 PE=4 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 7.7e-82
Identity = 176/469 (37.53%), Postives = 266/469 (56.72%), Query Frame = 0

Query: 1   MERLEKAHNLPWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRC 60
           + RL+   NLPW   GDFNE++  +EK+    +  R +    D +  C+ +DLGF G   
Sbjct: 488 LRRLKSQSNLPWCCMGDFNELVRLEEKQGRLGRSDRQMQLFRDVLDECSFVDLGFTGPSF 547

Query: 61  TWAKNKSNHGATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKID-------- 120
           TW  N+     T ERLDR +A P  L     +RV HL+   SDH+ + +  D        
Sbjct: 548 TWTNNRVG-DMTWERLDRAVATPDWLLRFPSIRVHHLEAQWSDHKPLWVTSDPMMTPHRK 607

Query: 121 ---------------------WGDTNQQQSFHKRLAKLE------QSWLQ---GITRKEL 180
                                W    Q    H  + K+       ++W +   G  RK++
Sbjct: 608 PFRFEEVWTSNPGCEEAIAESWRSAKQGAPMHIVVDKIRACRRGLRAWSKNHFGSIRKQI 667

Query: 181 -EIKRLSNPKDQAALDLLSKAEW-----ELEKLLEEEESYWHMRAREEWLKSGDKNTKWF 240
            E++ L    +  ++  ++  ++     +L+ LL +EE  W  R+R EWLK+GD+NT++F
Sbjct: 668 SEVELLLQQAEAMSMRGINHGQYKHLKGKLQALLSKEERLWRQRSRAEWLKAGDQNTRYF 727

Query: 241 HSKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNREDTKEVTKAIT 300
           H +ATQRK+RN++  + +  G+W     ++      YY+SL T+S+P   D   V + I 
Sbjct: 728 HCRATQRKRRNQVHRLKDPNGVWTTSQGQVSQLFVNYYSSLFTTSNPALVD--PVVENIM 787

Query: 301 KKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGEDTVKTCLG 360
             ++    + L + F+ +EV +A+K M+P KAPGPDG   + +QN W  +GED +   LG
Sbjct: 788 PSVTTTMNSMLVSDFTADEVVQALKQMAPLKAPGPDGLPPVFYQNYWHLIGEDVIAAVLG 847

Query: 361 ILNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALANRMKQVLDTII 420
            LN+ + +  IN T + LIPK K+P+ + EFRPISLCNVIYK+I+K LANR+K +L  I+
Sbjct: 848 SLNSGKILRAINHTYVTLIPKVKNPEVVTEFRPISLCNVIYKIISKVLANRLKTILPQIV 907

Query: 421 SPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKAYD 426
           S SQSAF+PGR ITDN+LV FE +H + +++TG+TG +A+KLDMSKAYD
Sbjct: 908 SESQSAFVPGRLITDNILVAFETLHYMQHQKTGKTGSMALKLDMSKAYD 953

BLAST of Lag0018773 vs. ExPASy TrEMBL
Match: F4NCI4 (Reverse transcriptase domain-containing protein OS=Beta vulgaris subsp. vulgaris OX=3555 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 1.3e-81
Identity = 175/468 (37.39%), Postives = 255/468 (54.49%), Query Frame = 0

Query: 1   MERLEKAHNLPWIIGGDFNEIMFNKEKKRGYPKPLRFINDLCDSIRICNLIDLGFIGDRC 60
           +  L +A   PW+ GGDFN ++   EKK G     R  +   +++  C+ +DLGF+G   
Sbjct: 126 LSALARASRRPWLCGGDFNLMLVASEKKGGDGFNSREADIFRNAMEECHFMDLGFVGYEF 185

Query: 61  TWAKNKSNHGATRERLDRYLANPKMLDIVKDMRVEHLQFHHSDHRAILLKIDWGDTNQQQ 120
           TW  N+      +ERLDR++AN           V HL    SDH  I+  +    +   +
Sbjct: 186 TWTNNRGGDANIQERLDRFVANDLWKIKFPGSFVSHLPKRKSDHVPIVASVKGAQSAATR 245

Query: 121 SFHKRLAKLEQSWLQ------------------GITRKELEIKRLSNPKD---------- 180
           +   +  + E  WL+                  GI       K LS  K           
Sbjct: 246 TKKSKRFRFEAMWLREGESDEVVKETWMRGTDAGINLARTANKLLSWSKQKFGHVAKEIR 305

Query: 181 --QAALDLLSKAE-------------WELEKLLEEEESYWHMRAREEWLKSGDKNTKWFH 240
             Q  + +L ++E               +++L + EE YWH R+R++W+KSGDKNTK+FH
Sbjct: 306 MCQHQMKVLMESEPSEDNIMHMRALDARMDELEKREEVYWHQRSRQDWIKSGDKNTKFFH 365

Query: 241 SKATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHPNREDTKEVTKAITK 300
            KA+ R++RN ++ I N  G W ED  ++      Y+ +L  S   N  +   +   +  
Sbjct: 366 QKASHREQRNNVRRIRNEAGEWFEDEDDVTECFAHYFENLFQSG--NNCEMDPILNIVKP 425

Query: 301 KISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGEDTVKTCLGI 360
           +I+++   +LDA F REEV  A+  M P+KAPGPDG +A+ +Q+ W+T+GED     L +
Sbjct: 426 QITDELGTQLDAPFRREEVSAALAQMHPNKAPGPDGMNALFYQHFWDTIGEDVTTKVLNM 485

Query: 361 LNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVIAKALANRMKQVLDTIIS 420
           LNN + I  +N+T I LIPK K  ++  +FRPISLCNV+YK++AK LANRMK VL  +I 
Sbjct: 486 LNNVDNIGAVNQTHIVLIPKKKHCESPVDFRPISLCNVLYKIVAKVLANRMKMVLPMVIH 545

Query: 421 PSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKAYD 426
            SQS F+PGR ITDNVLV +EC H L  ++TG+ GY+ +KLDMSKAYD
Sbjct: 546 ESQSGFVPGRLITDNVLVAYECFHFLRKKKTGKKGYLGLKLDMSKAYD 591

BLAST of Lag0018773 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-17
Identity = 58/224 (25.89%), Postives = 107/224 (47.77%), Query Frame = 0

Query: 140 KELEIKRLSNPKDQA-ALDLLSKAEWELEKLLEEEESYWHMRAREEWLKSGDKNTKWFHS 199
           + ++ + L+NP D    ++ +++ +W         ES++  ++R +WL+ GD NT++FH 
Sbjct: 399 ESIQSQLLTNPSDSLFRVEHVARKKWNF--FAAALESFYRQKSRIKWLQDGDANTRFFHK 458

Query: 200 KATQRKKRNEIKGIFNSRGIWVEDIKEIGTATTEYYNSLLTSSHP--NREDTKEVTKAIT 259
                + +N IK +     + VE++ ++      YY  LL S       +  + +     
Sbjct: 459 VILANQAKNLIKFLRMDDDVRVENVTQVKEMIVAYYTHLLGSDSDILTPDSVQRIKDIHP 518

Query: 260 KKISEDQKAKLDASFSREEVEKAIKGMSPSKAPGPDGAHAMLFQNLWETMGEDTVKTCLG 319
            + ++   ++L A  S +E+  A+  M  +KAPGPD   A  F   W  + + T+     
Sbjct: 519 FRCNDTLASRLSALPSDKEITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKE 578

Query: 320 ILNNKEEIEPINKTLIALIPKTKDPKTMNEFRPISLCNVIYKVI 361
                  ++  N T I LIPK      ++ FRP+S C V+YK+I
Sbjct: 579 FFRTGHLLKRFNATAITLIPKVTGVDQLSMFRPVSCCTVVYKII 620

BLAST of Lag0018773 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 58.2 bits (139), Expect = 1.9e-08
Identity = 25/62 (40.32%), Postives = 42/62 (67.74%), Query Frame = 0

Query: 364 LANRMKQVLDTIISPSQSAFIPGRQITDNVLVGFECIHVLNNRRTGRTGYVAIKLDMSKA 423
           +  R+K ++  +I P+Q++FIPGR  TDN++   E +H +  R+ G  G++ +KLD+ KA
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSM-RRKKGVKGWMLLKLDLEKA 60

Query: 424 YD 426
           YD
Sbjct: 61  YD 61

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_030923330.12.5e-8239.35uncharacterized protein LOC115950239 [Quercus lobata][more]
PRQ56718.17.2e-8237.29putative RNA-directed DNA polymerase [Rosa chinensis][more]
CCA66036.12.7e-8137.39hypothetical protein [Beta vulgaris subsp. vulgaris][more]
XP_010673168.14.7e-8138.13PREDICTED: uncharacterized protein LOC104889608 [Beta vulgaris subsp. vulgaris][more]
XP_023908235.13.9e-8038.66uncharacterized protein LOC112019924 [Quercus suber][more]
Match NameE-valueIdentityDescription
P143812.2e-2527.69Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
P113695.3e-1929.35LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P085481.3e-1727.56LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
O003701.4e-1627.96LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
Q032742.5e-0528.66Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra... [more]
Match NameE-valueIdentityDescription
A0A2N9EX834.1e-8340.52Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A803NML17.0e-8337.20Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A2P6SDG43.5e-8237.29Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1... [more]
A0A2N9HE467.7e-8237.53Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS37832 PE=4 SV=1[more]
F4NCI41.3e-8137.39Reverse transcriptase domain-containing protein OS=Beta vulgaris subsp. vulgaris... [more]
Match NameE-valueIdentityDescription
AT1G43760.11.3e-1725.89DNAse I-like superfamily protein [more]
AT4G20520.11.9e-0840.32RNA binding;RNA-directed DNA polymerases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036691Endonuclease/exonuclease/phosphatase superfamilyGENE3D3.60.10.10Endonuclease/exonuclease/phosphatasecoord: 1..111
e-value: 3.1E-12
score: 48.8
IPR036691Endonuclease/exonuclease/phosphatase superfamilySUPERFAMILY56219DNase I-likecoord: 7..113
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 336..425
e-value: 4.3E-15
score: 55.8
NoneNo IPR availablePANTHERPTHR19446REVERSE TRANSCRIPTASEScoord: 65..425
NoneNo IPR availablePANTHERPTHR19446:SF440SUBFAMILY NOT NAMEDcoord: 65..425
NoneNo IPR availableCDDcd01650RT_nLTR_likecoord: 329..425
e-value: 4.125E-30
score: 113.925
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 276..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018773.1Lag0018773.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity