Lag0018729 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018729
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCalcium-binding EF-hand family protein
Locationchr5: 33430807 .. 33431163 (+)
RNA-Seq ExpressionLag0018729
SyntenyLag0018729
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTTGTCGCAAACCAATCGGGATTCGAGGACTGGCTGCCGACGATGGCGCAGAGGCTCGGCGGCGAGGGCCTGATTGGGGAGCTTTGCAATGGGTTCAATCTTCTGATGGACAGAGAGAAGGGGGTCATCGATTTCGAGAGCCTGAAGAGGAACGCCGCGGCGCTAGGGCTGGCGGATCTGAGCGATGACGATCTCAGAGCGATGTTGAGGGAGGGGGATTTCGACGGCGATGGCGCTCTCAATCAGATGGAGTTTTGTGTTCTGATGTTCAGATTGAGCCCTGAATTGATGGACGAGTCCTGGTTCTTGCTCGAGGAGGCTCTAGGCCAACAGTTTAAGGATTTTTGTTGA

mRNA sequence

ATGGCTTTTGTCGCAAACCAATCGGGATTCGAGGACTGGCTGCCGACGATGGCGCAGAGGCTCGGCGGCGAGGGCCTGATTGGGGAGCTTTGCAATGGGTTCAATCTTCTGATGGACAGAGAGAAGGGGGTCATCGATTTCGAGAGCCTGAAGAGGAACGCCGCGGCGCTAGGGCTGGCGGATCTGAGCGATGACGATCTCAGAGCGATGTTGAGGGAGGGGGATTTCGACGGCGATGGCGCTCTCAATCAGATGGAGTTTTGTGTTCTGATGTTCAGATTGAGCCCTGAATTGATGGACGAGTCCTGGTTCTTGCTCGAGGAGGCTCTAGGCCAACAGTTTAAGGATTTTTGTTGA

Coding sequence (CDS)

ATGGCTTTTGTCGCAAACCAATCGGGATTCGAGGACTGGCTGCCGACGATGGCGCAGAGGCTCGGCGGCGAGGGCCTGATTGGGGAGCTTTGCAATGGGTTCAATCTTCTGATGGACAGAGAGAAGGGGGTCATCGATTTCGAGAGCCTGAAGAGGAACGCCGCGGCGCTAGGGCTGGCGGATCTGAGCGATGACGATCTCAGAGCGATGTTGAGGGAGGGGGATTTCGACGGCGATGGCGCTCTCAATCAGATGGAGTTTTGTGTTCTGATGTTCAGATTGAGCCCTGAATTGATGGACGAGTCCTGGTTCTTGCTCGAGGAGGCTCTAGGCCAACAGTTTAAGGATTTTTGTTGA

Protein sequence

MAFVANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLADLSDDDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALGQQFKDFC
Homology
BLAST of Lag0018729 vs. NCBI nr
Match: XP_038903612.1 (calcium-binding protein KRP1-like [Benincasa hispida])

HSP 1 Score: 231.1 bits (588), Expect = 4.9e-57
Identity = 110/118 (93.22%), Postives = 115/118 (97.46%), Query Frame = 0

Query: 1   MAFVANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLA 60
           MAF A QSGF+DWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLA
Sbjct: 29  MAFGAEQSGFQDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLA 88

Query: 61  DLSDDDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALGQQFKDFC 119
           DLSD+DLR+MLREGDFDGDGALNQMEFCVLMFRLSP+LMDESWFLLEEAL Q+FKDFC
Sbjct: 89  DLSDEDLRSMLREGDFDGDGALNQMEFCVLMFRLSPDLMDESWFLLEEALNQEFKDFC 146

BLAST of Lag0018729 vs. NCBI nr
Match: XP_008452312.1 (PREDICTED: calcium-binding protein PBP1 [Cucumis melo])

HSP 1 Score: 230.3 bits (586), Expect = 8.4e-57
Identity = 110/118 (93.22%), Postives = 115/118 (97.46%), Query Frame = 0

Query: 1   MAFVANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLA 60
           MAF A +SGFEDWLPTMA+RLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLA
Sbjct: 1   MAFSAEKSGFEDWLPTMAERLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLA 60

Query: 61  DLSDDDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALGQQFKDFC 119
           DLSD+DLR+MLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEAL Q+FKDFC
Sbjct: 61  DLSDEDLRSMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALHQEFKDFC 118

BLAST of Lag0018729 vs. NCBI nr
Match: XP_022929391.1 (calcium-binding protein PBP1-like [Cucurbita moschata] >XP_023552392.1 calcium-binding protein PBP1 [Cucurbita pepo subsp. pepo] >KAG6577399.1 Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015477.1 Calcium-binding protein PBP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 226.1 bits (575), Expect = 1.6e-55
Identity = 108/118 (91.53%), Postives = 113/118 (95.76%), Query Frame = 0

Query: 1   MAFVANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLA 60
           MAFVA+QSGFEDWLP MA+RLGGEGLIGELCNGFNLL DREKGVIDFESLKRNAAALGLA
Sbjct: 1   MAFVADQSGFEDWLPKMAERLGGEGLIGELCNGFNLLKDREKGVIDFESLKRNAAALGLA 60

Query: 61  DLSDDDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALGQQFKDFC 119
           DLSD+DLR MLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLE+AL Q+FK FC
Sbjct: 61  DLSDEDLREMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEDALNQEFKGFC 118

BLAST of Lag0018729 vs. NCBI nr
Match: XP_004149304.1 (calcium-binding protein KRP1 [Cucumis sativus] >KGN56198.1 hypothetical protein Csa_010363 [Cucumis sativus])

HSP 1 Score: 225.3 bits (573), Expect = 2.7e-55
Identity = 107/118 (90.68%), Postives = 114/118 (96.61%), Query Frame = 0

Query: 1   MAFVANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLA 60
           MAF A +SGFEDWLPTMA+RLGGEGLIGELCNGFNLLM+REKGVIDFESLKRNAAALGL 
Sbjct: 1   MAFSAEKSGFEDWLPTMAERLGGEGLIGELCNGFNLLMNREKGVIDFESLKRNAAALGLG 60

Query: 61  DLSDDDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALGQQFKDFC 119
           DLSD+DLR+MLREGDFDGDGALNQMEFCVLMFRLSPELM+ESWFLLEEAL Q+FKDFC
Sbjct: 61  DLSDEDLRSMLREGDFDGDGALNQMEFCVLMFRLSPELMEESWFLLEEALHQEFKDFC 118

BLAST of Lag0018729 vs. NCBI nr
Match: XP_022985275.1 (calcium-binding protein PBP1 [Cucurbita maxima])

HSP 1 Score: 220.3 bits (560), Expect = 8.7e-54
Identity = 106/117 (90.60%), Postives = 111/117 (94.87%), Query Frame = 0

Query: 1   MAFVANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLA 60
           MAFVA+QSGFEDWLP MA+RLGGEGLIGELCNGFNLL DREKGVIDF SLKRNAAALGLA
Sbjct: 1   MAFVADQSGFEDWLPKMAERLGGEGLIGELCNGFNLLKDREKGVIDFSSLKRNAAALGLA 60

Query: 61  DLSDDDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALGQQFKDF 118
           DLSD+DLR MLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLE+AL Q+FK F
Sbjct: 61  DLSDEDLREMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEDALSQEFKGF 117

BLAST of Lag0018729 vs. ExPASy Swiss-Prot
Match: O81831 (Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.1e-38
Identity = 79/109 (72.48%), Postives = 92/109 (84.40%), Query Frame = 0

Query: 7   QSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAA-LGLADLSDD 66
           Q  F D+LPTMA  LGGEGLIGELCNGF LLMDREKGVI FESL+RNAAA LGL DL+D+
Sbjct: 16  QPNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDE 75

Query: 67  DLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALGQQF 115
           D+R M++EGDFD DGALNQMEFCVLMFRLSP+LM+ S  L+ E + ++F
Sbjct: 76  DVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

BLAST of Lag0018729 vs. ExPASy Swiss-Prot
Match: Q9LSQ6 (Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 5.5e-35
Identity = 71/106 (66.98%), Postives = 88/106 (83.02%), Query Frame = 0

Query: 10  FEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAA-ALGLADLSDDDLR 69
           F+D+ PTMA +LGGEGLI E+C GF LLMD++KGVI FESL+RNA+  LGL DL+DDD+R
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 70  AMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALGQQF 115
            M+ EGDFD DGALNQMEFCVLMFRLSPELM+ S  ++ E + ++F
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

BLAST of Lag0018729 vs. ExPASy Swiss-Prot
Match: Q9ZPX9 (Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 2.1e-26
Identity = 56/107 (52.34%), Postives = 79/107 (73.83%), Query Frame = 0

Query: 7   QSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLADLSDDD 66
           ++ +ED LP MA+++  E  + ELC GF+LL D E+ +I  ESL+RN+  LG+  +S +D
Sbjct: 19  ETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKED 78

Query: 67  LRAMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALGQQ 114
            + M+REGD DGDGALNQ EFCVLM RLSPE+M+++   LE+AL Q+
Sbjct: 79  AQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

BLAST of Lag0018729 vs. ExPASy Swiss-Prot
Match: Q2TBN3 (Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 8.0e-10
Identity = 35/86 (40.70%), Postives = 51/86 (59.30%), Query Frame = 0

Query: 10  FEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLADLSDDDLRA 69
           F D+L  M Q++  +    E+   F L  D E G I F++LKR A  LG  +LSD++L+ 
Sbjct: 86  FSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLSDEELQE 145

Query: 70  MLREGDFDGDGALNQMEFCVLMFRLS 96
           M+ E D DGDG +N+ EF  +M + S
Sbjct: 146 MIDEADRDGDGEVNEQEFLRIMKKTS 170

BLAST of Lag0018729 vs. ExPASy Swiss-Prot
Match: Q9R1K9 (Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-09
Identity = 34/86 (39.53%), Postives = 51/86 (59.30%), Query Frame = 0

Query: 10  FEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLADLSDDDLRA 69
           F D+L  M Q++  +    E+   F L  D E G I F++LKR A  LG  +L+D++L+ 
Sbjct: 86  FSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLTDEELQE 145

Query: 70  MLREGDFDGDGALNQMEFCVLMFRLS 96
           M+ E D DGDG +N+ EF  +M + S
Sbjct: 146 MIDEADRDGDGEVNEQEFLRIMKKTS 170

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903612.14.9e-5793.22calcium-binding protein KRP1-like [Benincasa hispida][more]
XP_008452312.18.4e-5793.22PREDICTED: calcium-binding protein PBP1 [Cucumis melo][more]
XP_022929391.11.6e-5591.53calcium-binding protein PBP1-like [Cucurbita moschata] >XP_023552392.1 calcium-b... [more]
XP_004149304.12.7e-5590.68calcium-binding protein KRP1 [Cucumis sativus] >KGN56198.1 hypothetical protein ... [more]
XP_022985275.18.7e-5490.60calcium-binding protein PBP1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O818311.1e-3872.48Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1[more]
Q9LSQ65.5e-3566.98Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1[more]
Q9ZPX92.1e-2652.34Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2[more]
Q2TBN38.0e-1040.70Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1[more]
Q9R1K91.8e-0939.53Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 66..94
e-value: 0.008
score: 25.4
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 41..93
e-value: 1.4E-20
score: 72.8
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 62..97
score: 10.859108
NoneNo IPR availableGENE3D1.10.238.10coord: 8..117
e-value: 2.1E-20
score: 74.7
NoneNo IPR availablePANTHERPTHR47319:SF6CALCIUM-BINDING PROTEIN KRP1coord: 5..117
IPR044205Calcium-binding protein KIC/PBP1/KRP1PANTHERPTHR47319CALCIUM-BINDING PROTEIN KICcoord: 5..117
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 75..87
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 10..100

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018729.1Lag0018729.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding