Lag0018431 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018431
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionnuclear intron maturase 3, mitochondrial
Locationchr5: 26985289 .. 26987580 (+)
RNA-Seq ExpressionLag0018431
SyntenyLag0018431
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTTCCACCTTAGAAAAATAGCGACCCTATTGAAACCCAAGCTCTCAACTTCCTTGAACAGCAAACTTTACTCTGATCTGCCATTGAAGCATTCCCCTCTGTCTCAACATTCTGCACAAACCCTCACAAAGCCCCAACTCAAGGCTCTGGTTCTCAGCCGATTCACACAGGGCAAGTTCCTCGACCTCATTCAAAATGTTGTCGCTTCTCCCTCTGTTCTTCTCACTGCCTCCCAAAACCTCATCACTCCGCTTCCAAGTAATCGCCTCAACGCTCACTTACCCATTTTCGATTTGGTCTCCAATCGCTTTTCGGTCCAGGACATGTCTCGGGAGCTCTACGAAAATCGTTTCGATGTTGGAGCTTGCTGTGTTCGGATGCTACCATCGGAGGAGAAAGGTGAGAGTCTGGTCTTACCGAACTTGAAATTGAAGGTCTTAATCGAGGCTATTAGGTTGGTCTTAGAAATTGTTTATGACGAACGATTTGTAACGTTCTCTTACGGCGGGCGTGTTGGTATGGGGCGGCACACTGCGATTAGGTACCTGAAGAACTCGGTGGAAAACCCTAGTTGGTGGTTCACCGTTGCATTTCATCGCAGAAAGTTCGATTCTGTACATGTAAATAAGTTGTGCTTATTGGTACAAGAGAAAATTAAGGATGATACTTTGATTTATATGTTAAAGAAACTGTTTGAACTGGGCGCAATTCAAATTGAATTGGGTGCTTCTTATATGGGAAGGGGTTTCCCTCAGGAAAGTGGTTTGTGTTCTATCTTATTTAATATATACTTGAATGGCTTTGATAAGGAAATTCAACAAATACGTCTTCAAAAGAATGAAGAAAATCCCAAGTTCAATCTGGACGATATTGTTTCTTTTCATAATCCGGTGAAAATATATGCTGTTAGGTATTTGGATGAGATATTAGTAATAACATCAGGGTCGAAGATGCTAACAATGGAGTTGAAAAGCCAGGTGCTTATGTATTTAGAAGGGAATTTAGAATTGGAGGTGGATCGCATGAATACTGCAATTCATAGCGCTGTCTCGGAGAAAATTAGTTTCTTAGGAATGGAACTACAAGCAGTACCACCTTCAGTTCTGCATCCACCAATGTCGGAAAAGGCAATCAGGGCACGGAAGAAGTACCTTAGACAGAAGGAAGTTAGAGCAATAGAATTGAGAAATGCCCGTGAGAGAAACAGGAAGAAATTGGGATTGAAGATATTGAGTCACGTGTTCAAAAAATTGAAGCAAACTGATGGCTTTAGATCTGAATTCCAAATTGAGAATGAAGTTAGAGAAATCTTCAGAAACTGGGCTGATGAAGTTGTGCAAGATTTCTTTGAGTCTTTGGAAGATAATGCAGAATGGCACCGTGTGCTGTCAGCTGGTGATTTCCTCTCTTTAAAACACATAAGAAATCAATTGCCAGAAGGTCTTGTGAATGCTTATGATAGGTTTCAAGATCAGGTAGACAAGCACTTGAATCCTGTAAAGGTCAAAAAGGAAAAGGCTAGGGAGGATGAAGAGAAAAGAGTGGAGGAAGAAGAAGAATATGCTAAACGAACAGTTGAGGACTTAACAAGGCTGTGCATCAAAGTTGATGCTCCTATAGAGCTTGTCAGGAAGGCAGTCAAGATGGTTGGGTTTACAAATAAAATGGGCCGCCCCCGGCCAATTAGCTCTCTCATTGTTCTTGAAGATACTGATATTATCAAGTGGTATGCCGGTGTAGGAAGAAGGTGGTTAGACTTCTTTTGTTGTTGTCATAACTATAAGATGGTCAAAACTATTGTAACTTACCATTTGAGGTTTTCTTGTATTTTGACACTGGCAGAAAAGCATGAATCGACCAAACGGGAAGCCATTAAACATTACAGTAAAGATTTGAAGGTCTTTGATTTGAATGGAAATGAAGAACTGCATTTCCCAACAGAAAGAGAAGTTAAGATGTTGGGAGATAGAAATCTTGCAGACCCATACCCTGTGGATGGGGCTTTATCTTTGTTTCTGATTAGATTAGCCAGTGATGAAGCTCCGTATCCTTGTATTGCTCATTTTTGCAATAGAACAGACTCTATTTTATATCGGGTCCGATTACTGCAAAGGACTCTGAATGTCAATCCATCTAATGGACTGGAATGGGTGAGAGGGATGGGAGTGATTCATGAAAGTTTAAATCAGAGATGTGTCCCTCTCTGTGCTGATCACATCAGTGATTTATACATGGGGAAAATCAACCTTGAAGACTTGGACTGCACCTTATCATTAGATATGGACTGA

mRNA sequence

ATGTTCTTCCACCTTAGAAAAATAGCGACCCTATTGAAACCCAAGCTCTCAACTTCCTTGAACAGCAAACTTTACTCTGATCTGCCATTGAAGCATTCCCCTCTGTCTCAACATTCTGCACAAACCCTCACAAAGCCCCAACTCAAGGCTCTGGTTCTCAGCCGATTCACACAGGGCAAGTTCCTCGACCTCATTCAAAATGTTGTCGCTTCTCCCTCTGTTCTTCTCACTGCCTCCCAAAACCTCATCACTCCGCTTCCAAGTAATCGCCTCAACGCTCACTTACCCATTTTCGATTTGGTCTCCAATCGCTTTTCGGTCCAGGACATGTCTCGGGAGCTCTACGAAAATCGTTTCGATGTTGGAGCTTGCTGTGTTCGGATGCTACCATCGGAGGAGAAAGGTGAGAGTCTGGTCTTACCGAACTTGAAATTGAAGGTCTTAATCGAGGCTATTAGGTTGGTCTTAGAAATTGTTTATGACGAACGATTTGTAACGTTCTCTTACGGCGGGCGTGTTGGTATGGGGCGGCACACTGCGATTAGGTACCTGAAGAACTCGGTGGAAAACCCTAGTTGGTGGTTCACCGTTGCATTTCATCGCAGAAAGTTCGATTCTGTACATGTAAATAAGTTGTGCTTATTGGTACAAGAGAAAATTAAGGATGATACTTTGATTTATATGTTAAAGAAACTGTTTGAACTGGGCGCAATTCAAATTGAATTGGGTGCTTCTTATATGGGAAGGGGTTTCCCTCAGGAAAGTGGTTTGTGTTCTATCTTATTTAATATATACTTGAATGGCTTTGATAAGGAAATTCAACAAATACGTCTTCAAAAGAATGAAGAAAATCCCAAGTTCAATCTGGACGATATTGTTTCTTTTCATAATCCGGTGAAAATATATGCTGTTAGGTATTTGGATGAGATATTAGTAATAACATCAGGGTCGAAGATGCTAACAATGGAGTTGAAAAGCCAGGTGCTTATGTATTTAGAAGGGAATTTAGAATTGGAGGTGGATCGCATGAATACTGCAATTCATAGCGCTGTCTCGGAGAAAATTAGTTTCTTAGGAATGGAACTACAAGCAGTACCACCTTCAGTTCTGCATCCACCAATGTCGGAAAAGGCAATCAGGGCACGGAAGAAGTACCTTAGACAGAAGGAAGTTAGAGCAATAGAATTGAGAAATGCCCGTGAGAGAAACAGGAAGAAATTGGGATTGAAGATATTGAGTCACGTGTTCAAAAAATTGAAGCAAACTGATGGCTTTAGATCTGAATTCCAAATTGAGAATGAAGTTAGAGAAATCTTCAGAAACTGGGCTGATGAAGTTGTGCAAGATTTCTTTGAGTCTTTGGAAGATAATGCAGAATGGCACCGTGTGCTGTCAGCTGGTGATTTCCTCTCTTTAAAACACATAAGAAATCAATTGCCAGAAGGTCTTGTGAATGCTTATGATAGGTTTCAAGATCAGGTAGACAAGCACTTGAATCCTGTAAAGGTCAAAAAGGAAAAGGCTAGGGAGGATGAAGAGAAAAGAGTGGAGGAAGAAGAAGAATATGCTAAACGAACAGTTGAGGACTTAACAAGGCTGTGCATCAAAGTTGATGCTCCTATAGAGCTTGTCAGGAAGGCAGTCAAGATGGTTGGGTTTACAAATAAAATGGGCCGCCCCCGGCCAATTAGCTCTCTCATTGTTCTTGAAGATACTGATATTATCAAGTGGTATGCCGGTGTAGGAAGAAGGTGGTTAGACTTCTTTTGTTGTTGTCATAACTATAAGATGGTCAAAACTATTGTAACTTACCATTTGAGGTTTTCTTGTATTTTGACACTGGCAGAAAAGCATGAATCGACCAAACGGGAAGCCATTAAACATTACAGTAAAGATTTGAAGGTCTTTGATTTGAATGGAAATGAAGAACTGCATTTCCCAACAGAAAGAGAAGTTAAGATGTTGGGAGATAGAAATCTTGCAGACCCATACCCTGTGGATGGGGCTTTATCTTTGTTTCTGATTAGATTAGCCAGTGATGAAGCTCCGTATCCTTGTATTGCTCATTTTTGCAATAGAACAGACTCTATTTTATATCGGGTCCGATTACTGCAAAGGACTCTGAATGTCAATCCATCTAATGGACTGGAATGGGTGAGAGGGATGGGAGTGATTCATGAAAGTTTAAATCAGAGATGTGTCCCTCTCTGTGCTGATCACATCAGTGATTTATACATGGGGAAAATCAACCTTGAAGACTTGGACTGCACCTTATCATTAGATATGGACTGA

Coding sequence (CDS)

ATGTTCTTCCACCTTAGAAAAATAGCGACCCTATTGAAACCCAAGCTCTCAACTTCCTTGAACAGCAAACTTTACTCTGATCTGCCATTGAAGCATTCCCCTCTGTCTCAACATTCTGCACAAACCCTCACAAAGCCCCAACTCAAGGCTCTGGTTCTCAGCCGATTCACACAGGGCAAGTTCCTCGACCTCATTCAAAATGTTGTCGCTTCTCCCTCTGTTCTTCTCACTGCCTCCCAAAACCTCATCACTCCGCTTCCAAGTAATCGCCTCAACGCTCACTTACCCATTTTCGATTTGGTCTCCAATCGCTTTTCGGTCCAGGACATGTCTCGGGAGCTCTACGAAAATCGTTTCGATGTTGGAGCTTGCTGTGTTCGGATGCTACCATCGGAGGAGAAAGGTGAGAGTCTGGTCTTACCGAACTTGAAATTGAAGGTCTTAATCGAGGCTATTAGGTTGGTCTTAGAAATTGTTTATGACGAACGATTTGTAACGTTCTCTTACGGCGGGCGTGTTGGTATGGGGCGGCACACTGCGATTAGGTACCTGAAGAACTCGGTGGAAAACCCTAGTTGGTGGTTCACCGTTGCATTTCATCGCAGAAAGTTCGATTCTGTACATGTAAATAAGTTGTGCTTATTGGTACAAGAGAAAATTAAGGATGATACTTTGATTTATATGTTAAAGAAACTGTTTGAACTGGGCGCAATTCAAATTGAATTGGGTGCTTCTTATATGGGAAGGGGTTTCCCTCAGGAAAGTGGTTTGTGTTCTATCTTATTTAATATATACTTGAATGGCTTTGATAAGGAAATTCAACAAATACGTCTTCAAAAGAATGAAGAAAATCCCAAGTTCAATCTGGACGATATTGTTTCTTTTCATAATCCGGTGAAAATATATGCTGTTAGGTATTTGGATGAGATATTAGTAATAACATCAGGGTCGAAGATGCTAACAATGGAGTTGAAAAGCCAGGTGCTTATGTATTTAGAAGGGAATTTAGAATTGGAGGTGGATCGCATGAATACTGCAATTCATAGCGCTGTCTCGGAGAAAATTAGTTTCTTAGGAATGGAACTACAAGCAGTACCACCTTCAGTTCTGCATCCACCAATGTCGGAAAAGGCAATCAGGGCACGGAAGAAGTACCTTAGACAGAAGGAAGTTAGAGCAATAGAATTGAGAAATGCCCGTGAGAGAAACAGGAAGAAATTGGGATTGAAGATATTGAGTCACGTGTTCAAAAAATTGAAGCAAACTGATGGCTTTAGATCTGAATTCCAAATTGAGAATGAAGTTAGAGAAATCTTCAGAAACTGGGCTGATGAAGTTGTGCAAGATTTCTTTGAGTCTTTGGAAGATAATGCAGAATGGCACCGTGTGCTGTCAGCTGGTGATTTCCTCTCTTTAAAACACATAAGAAATCAATTGCCAGAAGGTCTTGTGAATGCTTATGATAGGTTTCAAGATCAGGTAGACAAGCACTTGAATCCTGTAAAGGTCAAAAAGGAAAAGGCTAGGGAGGATGAAGAGAAAAGAGTGGAGGAAGAAGAAGAATATGCTAAACGAACAGTTGAGGACTTAACAAGGCTGTGCATCAAAGTTGATGCTCCTATAGAGCTTGTCAGGAAGGCAGTCAAGATGGTTGGGTTTACAAATAAAATGGGCCGCCCCCGGCCAATTAGCTCTCTCATTGTTCTTGAAGATACTGATATTATCAAGTGGTATGCCGGTGTAGGAAGAAGGTGGTTAGACTTCTTTTGTTGTTGTCATAACTATAAGATGGTCAAAACTATTGTAACTTACCATTTGAGGTTTTCTTGTATTTTGACACTGGCAGAAAAGCATGAATCGACCAAACGGGAAGCCATTAAACATTACAGTAAAGATTTGAAGGTCTTTGATTTGAATGGAAATGAAGAACTGCATTTCCCAACAGAAAGAGAAGTTAAGATGTTGGGAGATAGAAATCTTGCAGACCCATACCCTGTGGATGGGGCTTTATCTTTGTTTCTGATTAGATTAGCCAGTGATGAAGCTCCGTATCCTTGTATTGCTCATTTTTGCAATAGAACAGACTCTATTTTATATCGGGTCCGATTACTGCAAAGGACTCTGAATGTCAATCCATCTAATGGACTGGAATGGGTGAGAGGGATGGGAGTGATTCATGAAAGTTTAAATCAGAGATGTGTCCCTCTCTGTGCTGATCACATCAGTGATTTATACATGGGGAAAATCAACCTTGAAGACTTGGACTGCACCTTATCATTAGATATGGACTGA

Protein sequence

MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPLSQHSAQTLTKPQLKALVLSRFTQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNAHLPIFDLVSNRFSVQDMSRELYENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFELGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVSFHNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKHIRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKMLGDRNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD
Homology
BLAST of Lag0018431 vs. NCBI nr
Match: KAG6600779.1 (Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 683/769 (88.82%), Postives = 718/769 (93.37%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPL----SQHSAQTLTKPQLKALVLSRF 60
           M  HLRKIAT LKPKLS+S N KLYSD PLKHSPL     QHS QTLT+PQL+ALVLSRF
Sbjct: 1   MLIHLRKIATPLKPKLSSSFN-KLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRF 60

Query: 61  TQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNA--HLPIFDLVSNRFSVQDMSREL 120
           +QGKF DL+QNVVASPSVL TASQNLITPLPSNRLNA   L   D+VS+ FSV+DM+REL
Sbjct: 61  SQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMAREL 120

Query: 121 YENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVG 180
           YENRFDVGACCVR+ PSEEKGE LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVG
Sbjct: 121 YENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVG 180

Query: 181 MGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFE 240
           MGRHTAIRYLKNSV+NPSWWFTVAF RRKFDSVHVNKLCLLVQEKIKDD LI MLKKLFE
Sbjct: 181 MGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFE 240

Query: 241 LGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVS 300
           L A+QIELG  Y+GRGFPQESGLCSIL NIY NGFDKEIQQIRLQKNEENPKF++D  VS
Sbjct: 241 LEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSMDGTVS 300

Query: 301 FHNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEK 360
           FHNPVKIYAVRYLDEILVITSGSKML MELKSQVL YLEGNLELEVDRMNTAIHSAVSEK
Sbjct: 301 FHNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEK 360

Query: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420
           ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH
Sbjct: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420

Query: 421 VFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKH 480
           VFKKLK+TDG +SEFQIE EV EIFRNWADEVV+DFFESLEDN EWHR LSAGDFLSLKH
Sbjct: 421 VFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKH 480

Query: 481 IRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLC 540
           IRNQLP  LVNAYDRFQDQV++HLNP+K K+EKAREDEEKR+ EEE YAKRTVEDLTRLC
Sbjct: 481 IRNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLC 540

Query: 541 IKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 600
           IKV+APIELVRKAVKM+GFTNKMGRPRPISSLI LEDTDIIKWYAGVGRRWLDFFCCCHN
Sbjct: 541 IKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHN 600

Query: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKM 660
           YKMVKTIVTYHLRFSCILTLAEKHESTKREA+KHYSKDLKVFDLNG EE+HFPTEREVKM
Sbjct: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKM 660

Query: 661 LGDRNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSN 720
           LG+RNLADPYPVDGA SLFLIRL +DE  YPCIAHFCNRTDSILYRVRLLQ+TLNVNPSN
Sbjct: 661 LGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSN 720

Query: 721 GLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
           G+EWVRGMGVIHESLNQRC+PLCADHISDLYMGKINL+DLDCTLSLDMD
Sbjct: 721 GVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Lag0018431 vs. NCBI nr
Match: KAG7031416.1 (Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1344.7 bits (3479), Expect = 0.0e+00
Identity = 683/769 (88.82%), Postives = 718/769 (93.37%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPL----SQHSAQTLTKPQLKALVLSRF 60
           M  HLRKIAT LKPKLS+S N KLYSD PLKHSPL     QHS QTLT+PQL+ALVLSRF
Sbjct: 1   MLIHLRKIATPLKPKLSSSFN-KLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRF 60

Query: 61  TQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNA--HLPIFDLVSNRFSVQDMSREL 120
           +QGKF DL+QNVVASPSVL TASQNLITPLPSNRLNA   L   D+VS+ FSV+DM+REL
Sbjct: 61  SQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMAREL 120

Query: 121 YENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVG 180
           YENRFDVGACCVR+ PSEEKGE LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVG
Sbjct: 121 YENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVG 180

Query: 181 MGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFE 240
           MGRHTAIRYLKNSV+NPSWWFTVAF RRKFDSVHVNKLCLLVQEKIKDD LI MLKKLFE
Sbjct: 181 MGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFE 240

Query: 241 LGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVS 300
           L A+QIELG  Y+GRGFPQESGLCSIL NIY NGFDKEIQQIRLQKNEENPKF+LD  VS
Sbjct: 241 LEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVS 300

Query: 301 FHNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEK 360
           FHNPVKIYAVRYLDEILVITSGSKML MELKSQVL YLEGNLELEVDRMNTAIHSAVSEK
Sbjct: 301 FHNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEK 360

Query: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420
           ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH
Sbjct: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420

Query: 421 VFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKH 480
           VFKKLK+TDG +SEFQIE EV EIFRNWADEVV+DFFESLEDN EWHR LSAGDFLSLKH
Sbjct: 421 VFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKH 480

Query: 481 IRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLC 540
           IRNQLP  LVNAYDRFQDQV++HLNP+K K+EKAREDEEKR+ EEE YAKRTVEDLTRLC
Sbjct: 481 IRNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLC 540

Query: 541 IKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 600
           IKV+APIELVRKAVKM+GFTNKMGRPRPISSLI LEDTDIIKWYAGVGRRWLDFFCCCHN
Sbjct: 541 IKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHN 600

Query: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKM 660
           YKMVKTIVTYHLRFSCILTLAEKHESTKREA+KHYSKDLKVFDLNG EE+HFPTEREVKM
Sbjct: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKM 660

Query: 661 LGDRNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSN 720
           LG+RNLADPYPVDGA SLFLIRL +DE  YPCIAHFCNRTDSILYRVRLLQ+TLNVNPSN
Sbjct: 661 LGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSN 720

Query: 721 GLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
           G+EWVRGMGVIHESLNQRC+PLCADHI+DLYMGKINL+DLDCTLSLDMD
Sbjct: 721 GVEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 768

BLAST of Lag0018431 vs. NCBI nr
Match: XP_022976163.1 (nuclear intron maturase 3, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1339.3 bits (3465), Expect = 0.0e+00
Identity = 681/769 (88.56%), Postives = 717/769 (93.24%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPL----SQHSAQTLTKPQLKALVLSRF 60
           M  HLRKIAT LKPKLS+SLN KLYSD PLKHSPL     QHS QTLT+PQL+ALVLSRF
Sbjct: 1   MLIHLRKIATPLKPKLSSSLN-KLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRF 60

Query: 61  TQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNA--HLPIFDLVSNRFSVQDMSREL 120
           +QGKF DL+QNVVASPSVL TASQNLITPLPSNRLNA   L   D+VS+ FSV+DM+REL
Sbjct: 61  SQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMAREL 120

Query: 121 YENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVG 180
           YENRFDVGACCVR+  SEEKGE LVLPNLKLKVL+EA+++VLEIVYDERFVTFSYGGRVG
Sbjct: 121 YENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAMKMVLEIVYDERFVTFSYGGRVG 180

Query: 181 MGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFE 240
           MGRHTAIRYLKNSV+NPSWWFTVAF RRKFDSVHVNKLCLLVQEKIKDD LI MLKKLFE
Sbjct: 181 MGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFE 240

Query: 241 LGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVS 300
           L A+QIELG  Y+GRGFPQESGLCSIL NIY NGFDKEIQQIRLQKNEENPKF+L+  VS
Sbjct: 241 LEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLNGTVS 300

Query: 301 FHNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEK 360
           FHNPVKIYAVRYLDEILVITSGSKM  MELKSQVL YLEGNLELEVDRMNTAIHSAVSEK
Sbjct: 301 FHNPVKIYAVRYLDEILVITSGSKMQIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEK 360

Query: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420
           ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH
Sbjct: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420

Query: 421 VFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKH 480
           VFKKLK+TDG +SEFQIE EV EIFRNWADEVV+DFFESLEDN EWHR LSAGDFLSLKH
Sbjct: 421 VFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKH 480

Query: 481 IRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLC 540
           IRNQLP  LVNAYDRFQDQV+KHLNP+K KKEKAREDEEKR+ EEE YAKRTVEDLTRLC
Sbjct: 481 IRNQLPVDLVNAYDRFQDQVNKHLNPLKAKKEKAREDEEKRLVEEERYAKRTVEDLTRLC 540

Query: 541 IKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 600
           IKV+APIELVRKAVKM+GFTNKMGRP+PISSLI LEDTDIIKWYAGVGRRWLDFFCCCHN
Sbjct: 541 IKVEAPIELVRKAVKMIGFTNKMGRPQPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHN 600

Query: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKM 660
           YKMVKTIVTYHLRFSCILTLAEKHESTKREA+KHYSKDLKVFDLNG EE+HFPTEREVKM
Sbjct: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKM 660

Query: 661 LGDRNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSN 720
           LG+RNLADPYPVDGA SLFLIRL +DE  YPCIAHFCNRTDSILYRVRLLQ+TLNVNPSN
Sbjct: 661 LGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSN 720

Query: 721 GLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
           G+EWVRGMGVIHESLNQRC+PLCADHISDLYMGKINL+DLDCTLSLDMD
Sbjct: 721 GVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Lag0018431 vs. NCBI nr
Match: XP_022942578.1 (nuclear intron maturase 3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1335.5 bits (3455), Expect = 0.0e+00
Identity = 680/769 (88.43%), Postives = 715/769 (92.98%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPL----SQHSAQTLTKPQLKALVLSRF 60
           M  HLRKIAT LKPKLS+S N KLYSD PLKHSPL     QHS QTLT+PQL+ALVLSRF
Sbjct: 1   MLIHLRKIATPLKPKLSSSFN-KLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRF 60

Query: 61  TQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNA--HLPIFDLVSNRFSVQDMSREL 120
           +QGKF DL+QNVVASPSVL TASQNLITPLPSNRLNA   L   D+VS+ FSV+DM+REL
Sbjct: 61  SQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSLDMVSSCFSVEDMAREL 120

Query: 121 YENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVG 180
           YENRFDVGACCVR+  SEEKGE LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVG
Sbjct: 121 YENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVG 180

Query: 181 MGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFE 240
           MGRHTAIRYLKNSV+NPSWWFTVAF RRKFDSVHVNKLCLLVQEKIKDD LI MLKKLFE
Sbjct: 181 MGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFE 240

Query: 241 LGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVS 300
           L A+QIELG  Y+GRG PQESGLCSIL NIY NGFDKEIQQIRL+KNEENPKF+LD  VS
Sbjct: 241 LEAVQIELGGCYLGRGLPQESGLCSILSNIYFNGFDKEIQQIRLEKNEENPKFSLDGTVS 300

Query: 301 FHNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEK 360
           FHNPVKIYAVRYLDEILVITSGSKML MELKSQVL YLEGNLELEVDRMNTAIHSAVSEK
Sbjct: 301 FHNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEK 360

Query: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420
           ISFLGMELQAV PSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH
Sbjct: 361 ISFLGMELQAVLPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420

Query: 421 VFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKH 480
           VFKKLK+TDG +SEFQIE EV EIFRNWADEVV+DFFESLEDN EWHR LSAGDFLSLKH
Sbjct: 421 VFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKH 480

Query: 481 IRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLC 540
           IRNQLP  LVNAYDRFQDQV++HLNP+K K+EKAREDEEKR+ EEE YAKRTVEDLTRLC
Sbjct: 481 IRNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLC 540

Query: 541 IKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 600
           IKV+APIELVRKAVKM+GFTNKMGRPRPISSLI LEDTDIIKWYAGVGRRWLDFFCCCHN
Sbjct: 541 IKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHN 600

Query: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKM 660
           YKMVKTIVTYHLRFSCILTLAEKHESTKREA+KHYSKDLKVFDLNG EE+HFPTEREVKM
Sbjct: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKM 660

Query: 661 LGDRNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSN 720
           LG+RNLADPYPVDGA SLFLIRL +DE  YPCIAHFCNRTDSILYRVRLLQ+TLNVNPSN
Sbjct: 661 LGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSN 720

Query: 721 GLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
           G+EWVRGMGVIHESLNQRC+PLCADHISDLYMGKINL+DLDCTLSLDMD
Sbjct: 721 GVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Lag0018431 vs. NCBI nr
Match: XP_023544815.1 (nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544823.1 nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544830.1 nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544839.1 nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1328.9 bits (3438), Expect = 0.0e+00
Identity = 675/769 (87.78%), Postives = 712/769 (92.59%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPL----SQHSAQTLTKPQLKALVLSRF 60
           M  HLRKIAT  KP LS+S N KLYSD PLKHSPL     QHS QTLT+PQL+ALVLSRF
Sbjct: 1   MLIHLRKIATPWKPNLSSSFN-KLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRF 60

Query: 61  TQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNA--HLPIFDLVSNRFSVQDMSREL 120
           +QGKF DL++NVVASPSVL TASQNLITPLPSNRLNA   L  FD+VS+ FSV+DM+REL
Sbjct: 61  SQGKFFDLLRNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSFDMVSSCFSVEDMAREL 120

Query: 121 YENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVG 180
           YENRFDVGACCVR+  SEEKGE LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVG
Sbjct: 121 YENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVG 180

Query: 181 MGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFE 240
           MGRHTAIRYLKNSV+NPSWWFTVAF RRKFDSVHVNKLCLLVQEKIKDD LI MLKKLFE
Sbjct: 181 MGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFE 240

Query: 241 LGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVS 300
           L A+QIELG  Y+GRGFPQESGLCSIL NIY NGFDKE+QQIRL+KNEENPKF+LD  VS
Sbjct: 241 LEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKELQQIRLEKNEENPKFSLDGTVS 300

Query: 301 FHNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEK 360
           FHNPVKIYAVRYLDEILVITSGSKML MELKSQVL YLEGNLELEVDRMNTAIHSAVSEK
Sbjct: 301 FHNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEK 360

Query: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420
           ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKIL H
Sbjct: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILGH 420

Query: 421 VFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKH 480
           VFKKLK+TDG + EFQIE EV EIFRNWADEVV+DF ESLEDN EWHR LSAGDFLSLKH
Sbjct: 421 VFKKLKRTDGLKYEFQIEKEVTEIFRNWADEVVRDFLESLEDNTEWHRPLSAGDFLSLKH 480

Query: 481 IRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLC 540
           IRNQLP  LVNAYDRFQDQV++HLNP+K KKEKAREDE+KR+ EEE YAKRTVEDLTRLC
Sbjct: 481 IRNQLPVDLVNAYDRFQDQVNQHLNPLKAKKEKAREDEKKRLGEEERYAKRTVEDLTRLC 540

Query: 541 IKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 600
           IKV+APIELVRKAVKM+GFTNKMGRPRPISSLI LEDTDIIKWYAGVGRRWLDFFCCCHN
Sbjct: 541 IKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHN 600

Query: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKM 660
           YK VKTIVTYHLRFSCILTLAEKHESTKREA+KHYSKDLKVFDLNG EE+HFPTEREVKM
Sbjct: 601 YKTVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKM 660

Query: 661 LGDRNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSN 720
           LG+RNLADPYPVDGA SLFLIRL +DE  YPCIAHFCNRTDSILYRVRLLQ+TLNVNPSN
Sbjct: 661 LGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSN 720

Query: 721 GLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
           G+EWVRGMGVIHESLNQRC+PLCADHISDLYMGKINL+DLDCTLSLDMD
Sbjct: 721 GVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Lag0018431 vs. ExPASy Swiss-Prot
Match: Q9LZA5 (Nuclear intron maturase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT3 PE=3 SV=2)

HSP 1 Score: 831.6 bits (2147), Expect = 7.0e-240
Identity = 442/740 (59.73%), Postives = 546/740 (73.78%), Query Frame = 0

Query: 33  SPLSQHSAQTLTKP----QLKALVLSRFTQGKFLDLIQNVVASPSVLLTASQNLITPLPS 92
           S L  +S QT+T+P    +L+ALVL +++ GKF  L++N V+ P VLL A QNL     S
Sbjct: 30  SSLFLNSDQTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNL-----S 89

Query: 93  NRLNAHLPIFDLVSNRFSVQDMSRELYENRFDVGACCVRMLPSEEKGESLVLPNLKLKVL 152
              N+   + D VS RFS+++M RE+ E RFD+ +CCV  + S     SLVLPNLKLKVL
Sbjct: 90  LSANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISS-----SLVLPNLKLKVL 149

Query: 153 IEAIRLVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVH 212
           IEAIR+VLEIVYD+RF TFSYGGRVGMGRHTAIRYLKNSVENP WWF V+F R  F+  +
Sbjct: 150 IEAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERN 209

Query: 213 VNKLCLLVQEKIKDDTLIYMLKKLFELGAIQIELGASYMGRGFPQESGLCSILFNIYLNG 272
           V+ LC  V EKI D  LI M+KKLFE G ++IELG    GRGFPQE GLCSIL N+Y +G
Sbjct: 210 VDILCGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDG 269

Query: 273 FDKEIQQIRLQKNEENPKFNLDDIVS----FHNPVKIYAVRYLDEILVITSGSKMLTMEL 332
            DKEIQ +RL+   +NP+    D  S    F  PV IYAVRYLDEILVITSGSKMLTM+L
Sbjct: 270 LDKEIQDLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDL 329

Query: 333 KSQVLMYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRARKK 392
           K +++  LE  LEL VDR+NT+IHSAVSEKI+FLGM LQAVPPSVL PP SEKA+RA KK
Sbjct: 330 KKRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKK 389

Query: 393 YLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTDGFRSEFQIENEVREIFRNWAD 452
           Y RQK+VR +ELRNARERNRK LGLKI  HV KK+KQ++GF+ E +IENEVR+IF++W +
Sbjct: 390 YQRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGE 449

Query: 453 EVVQDFFESLEDNAEWHRVLSAGDFLSLKHIRNQLPEGLVNAYDRFQDQVDKHLNPVKVK 512
           EV+QDF  SLE+  +WH +L+ GDFLSL+HIR +LP+ L++AYD FQ+QVDKHL P + K
Sbjct: 450 EVMQDFMGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK 509

Query: 513 KEKAREDEEKRVEEEEE--YAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPRP 572
             K  EDEE+RVEEEEE  YA+RTVEDLT+LC+KV AP ELVRKA+K+VGFTN MGRPRP
Sbjct: 510 --KVLEDEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAIKLVGFTNSMGRPRP 569

Query: 573 ISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSCILTLAEKHESTK 632
           I  L+ LED+DIIKWYA                                    EKH STK
Sbjct: 570 IIHLVTLEDSDIIKWYA----------------------------------RHEKHGSTK 629

Query: 633 REAIKHYSKDLKVFDLNGNEELHFPTEREVKMLGDRNLADPYPVDGALSLFLIRLASDEA 692
           +  I+HY+KDL+V DL+G EE HFP+EREVKM+GD+NL+DP PVDG LSL LIRLASDE 
Sbjct: 630 K-LIRHYTKDLRVSDLDGREEAHFPSEREVKMMGDKNLSDPKPVDGTLSLLLIRLASDEP 689

Query: 693 PYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGLEWVRGMGVIHESLNQRCVPLCADHIS 752
            + C A FC R+D+I++RV LLQ  L++NP +  +WV GMG IH +LN++C+PLC+ HIS
Sbjct: 690 LHHCAASFCERSDTIMHRVHLLQNRLHINPLDEEKWVPGMGTIHSALNRKCLPLCSTHIS 722

Query: 753 DLYMGKINLEDLDCTLSLDM 763
           D+Y+GKI L+D+D +  +D+
Sbjct: 750 DVYLGKITLQDVDSSSFIDL 722

BLAST of Lag0018431 vs. ExPASy Swiss-Prot
Match: Q9CA78 (Nuclear intron maturase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT4 PE=3 SV=2)

HSP 1 Score: 226.5 bits (576), Expect = 1.0e-57
Identity = 194/764 (25.39%), Postives = 346/764 (45.29%), Query Frame = 0

Query: 34  PLSQHSAQTLTKPQLKALVLSRFTQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNA 93
           P S+   +   + +LK  V  +   GKF DL++ V+A P  L  A   +       RLN+
Sbjct: 77  PRSRMELKRSLELRLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCI-------RLNS 136

Query: 94  HLPIFDLVSNRFSVQDMSRELYENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIR 153
           ++ I +  +   +   ++ EL    FDV +    ++  ++  E LVLP++ LKV+ EAIR
Sbjct: 137 NVSITER-NGSVAFDSIAEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIR 196

Query: 154 LVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLC 213
           +VLE+V+   F   S+  R G GR +A++Y+ N++    W FT++ +++   SV  N L 
Sbjct: 197 IVLEVVFSPHFSKISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLS 256

Query: 214 LLVQEKIKDDTLIYMLKKLFELGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEI 273
           ++ +EK++D +L  +L+ +FE   + +E G    G G PQE  L  +L NIYL+ FD E 
Sbjct: 257 VM-EEKVEDSSLSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEF 316

Query: 274 QQIRL---------QKNEENP-------------KFNLDDIVSFHNPVKIYAVRYLDEIL 333
            +I +         + +E++P             +  L         +++Y  R++DEI 
Sbjct: 317 YRISMRHEALGLDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVALRVYCCRFMDEIY 376

Query: 334 VITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLH 393
              SG K +  +++S+ + +L  +L L++           +  +  LG     V  +V  
Sbjct: 377 FSVSGPKKVASDIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG---TLVRKNVRE 436

Query: 394 PPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTD-------- 453
            P + KA+   K+ +R   + A++   A      ++G K L H  KK+K+++        
Sbjct: 437 SP-TVKAVHKLKEKVR---LFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKGLADSN 496

Query: 454 --------GFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKH- 513
                     ++  + ++  + + R W ++V++    +  D +E        +F+  KH 
Sbjct: 497 STLSQISCHRKAGMETDHWYKILLRIWMEDVLR----TSADRSE--------EFVLSKHV 556

Query: 514 IRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLC 573
           +   +P+ L +A+ +FQ+    +++      E      +               D     
Sbjct: 557 VEPTVPQELRDAFYKFQNAAAAYVSSETANLEALLPCPQS-------------HDRPVFF 616

Query: 574 IKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 633
             V AP   + + +   G     G  R  S LI+L+   II WY+G+ RRW+ ++  C N
Sbjct: 617 GDVVAPTNAIGRRLYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSN 676

Query: 634 YKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKM 693
           +  +K ++   +R SCI TLA K+   + E  K    +L       + E     E+    
Sbjct: 677 FDEIKALIDNQIRMSCIRTLAAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSP 736

Query: 694 LGDRNLADPYPV--DGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNP 753
             DR+    Y +   G   L L RL S+  P  C    C+     +Y +  ++R      
Sbjct: 737 AFDRDEHLTYGLSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------ 793

Query: 754 SNGLEWVRGMGV-IHESLNQRCVPLCADHISDLYMGKINLEDLD 756
                W  G  V I  SLN R + LC  H+ DLY+G+I+L+ +D
Sbjct: 797 QKFPGWKTGFSVCIPSSLNGRRIGLCKQHLKDLYIGQISLQAVD 793

BLAST of Lag0018431 vs. ExPASy Swiss-Prot
Match: P05511 (Uncharacterized 91 kDa protein in cob intron OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPMIT.06 PE=4 SV=4)

HSP 1 Score: 80.9 bits (198), Expect = 7.0e-14
Identity = 70/252 (27.78%), Postives = 118/252 (46.83%), Query Frame = 0

Query: 146 KVLIEAIRLVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFD 205
           K++ E +R+VLE +Y+  F T S+G R G   H+A+R +  + +  +WW      +  FD
Sbjct: 318 KLVQEILRIVLEAIYEPLFNTASHGFRPGRSCHSALRSIFTNFKGCTWWIEGDI-KACFD 377

Query: 206 SVHVNKLCLLVQEKIKDDTLIYMLKKLFELGAIQIELGASYMGRGFPQESGLCSILFNIY 265
           S+  +KL  L+  KIKD   I +++K    G +  E    Y   G PQ S +  IL NIY
Sbjct: 378 SIPHDKLIALLSSKIKDQRFIQLIRKALNAGYL-TENRYKYDIVGTPQGSIVSPILANIY 437

Query: 266 LNGFDKEIQQIRLQKNEENP--------------------KFNLDD------IVSFHN-P 325
           L+  D+ I+ ++ + + + P                    + N D        +   N P
Sbjct: 438 LHQLDEFIENLKSEFDYKGPIARKRTSESRHLHYLMAKAKRENADSKTIRKIAIEMRNVP 497

Query: 326 VKIYA--------VRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSA 363
            KI+         VRY D+ +V  +GS   T E+ +++  +   ++ L V    T I ++
Sbjct: 498 NKIHGIQSNKLMYVRYADDWIVAVNGSYTQTKEILAKITCFC-SSIGLTVSPTKTKITNS 557

BLAST of Lag0018431 vs. ExPASy Swiss-Prot
Match: P03876 (Putative COX1/OXI3 intron 2 protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AI2 PE=4 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 9.1e-14
Identity = 71/291 (24.40%), Postives = 130/291 (44.67%), Query Frame = 0

Query: 110 MSRELYENRFDVGACCVRMLPSEEKG-ESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFS 169
           +S+++  N F         +P    G   L + N + K++ E++R++LEI+Y+  F  +S
Sbjct: 329 LSKDINTNMFKFSPVRRVEIPKTSGGFRPLSVGNPREKIVQESMRMMLEIIYNNSFSYYS 388

Query: 170 YGGRVGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYM 229
           +G R  +   TAI   KN ++  +W+  V  + + FD++  N L  ++ E+IKD   + +
Sbjct: 389 HGFRPNLSCLTAIIQCKNYMQYCNWFIKVDLN-KCFDTIPHNMLINVLNERIKDKGFMDL 448

Query: 230 LKKLFELGAIQIELGASYMGRGFPQESGLCSILFNIYL--------NGFDKEIQQIRLQK 289
           L KL   G +           G PQ S +  IL NI+L        N F+ E     +  
Sbjct: 449 LYKLLRAGYVDKNNNYHNTTLGIPQGSVVSPILCNIFLDKLDKYLENKFENEFNTGNMSN 508

Query: 290 NEENPKFN-------------------------LDDIVSFHNPVKIYAVRYLDEILVITS 349
              NP +N                           ++ S  +  + Y VRY D+I++   
Sbjct: 509 RGRNPIYNSLSSKIYRCKLLSEKLKLIRLRDHYQRNMGSDKSFKRAYFVRYADDIIIGVM 568

Query: 350 GSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVP 367
           GS      + + +  +L+ NL + ++ M+ ++     E +SFLG +++  P
Sbjct: 569 GSHNDCKNILNDINNFLKENLGMSIN-MDKSVIKHSKEGVSFLGYDVKVTP 617


HSP 2 Score: 44.3 bits (103), Expect = 7.3e-03
Identity = 84/399 (21.05%), Postives = 155/399 (38.85%), Query Frame = 0

Query: 368 SVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNR---------KKLGLKILSHVFKK 427
           SV+ P +    +    KYL  K        N   R R         K    K+LS   K 
Sbjct: 474 SVVSPILCNIFLDKLDKYLENKFENEFNTGNMSNRGRNPIYNSLSSKIYRCKLLSEKLKL 533

Query: 428 LKQTDGFRSEFQIENEV-REIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKHIRN 487
           ++  D ++     +    R  F  +AD+++     S  D               L  I N
Sbjct: 534 IRLRDHYQRNMGSDKSFKRAYFVRYADDIIIGVMGSHNDCKN-----------ILNDINN 593

Query: 488 QLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDE-EKRVEEEEEYAKRTVEDLTRLCIK 547
            L E L  + +  +  +      V       +    EKR     +     +       + 
Sbjct: 594 FLKENLGMSINMDKSVIKHSKEGVSFLGYDVKVTPWEKRPYRMIKKGDNFIRVRHHTSLV 653

Query: 548 VDAPIELVRKAVKMVGFTNK--MGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 607
           V+API  +   +   G+ +   +G+PR +  LI  E   I+  Y  VGR  ++++    N
Sbjct: 654 VNAPIRSIVMKLNKHGYCSHGILGKPRGVGRLIHEEMKTILMHYLAVGRGIMNYYRLATN 713

Query: 608 YKMVKTIVTYHLRFSCILTLAEKHE-STKREAIKHYSKDLKVFDLNGNEELHFPTEREVK 667
           +  ++  +TY L +SC LTLA K + +T ++ I  + K L    ++ + ++ F  + + K
Sbjct: 714 FTTLRGRITYILFYSCCLTLARKFKLNTVKKVILKFGKVL----VDPHSKVSFSID-DFK 773

Query: 668 MLGDRNLADP-YPVDGALSLFLIRLASDEAPYPCIAHFC-NRTDSILYRVRLLQRTLNVN 727
           +    N+ D  Y  D  L  +   L    + +  I   C ++ D  ++ VR L    N  
Sbjct: 774 IRHKMNMTDSNYTPDEILDRYKYMLPRSLSLFSGICQICGSKHDLEVHHVRTLNNAANKI 833

Query: 728 PSNGLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKIN 751
                +++ G  +    +N++ + +C      ++ GK N
Sbjct: 834 KD---DYLLGRMI---KMNRKQITICKTCHFKVHQGKYN 850

BLAST of Lag0018431 vs. ExPASy Swiss-Prot
Match: P0A3U0 (Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris OX=1359 GN=ltrA PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 5.9e-13
Identity = 67/275 (24.36%), Postives = 122/275 (44.36%), Query Frame = 0

Query: 131 SEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVEN 190
           + +K   L +P    K++ EA+R++LE +Y+  F   S+G R     HTA++ +K     
Sbjct: 93  NSKKMRPLGIPTFTDKLIQEAVRIILESIYEPVFEDVSHGFRPQRSCHTALKTIKREF-G 152

Query: 191 PSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFELGAIQ-IELGASYMGR 250
            + WF     +  FD++    L  L+  KIKD  +  ++ K  + G ++  +   +Y G 
Sbjct: 153 GARWFVEGDIKGCFDNIDHVTLIGLINLKIKDMKMSQLIYKFLKAGYLENWQYHKTYSGT 212

Query: 251 GFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVSFHNPVK--------- 310
             PQ   L  +L NIYL+  DK + Q++++ + E+P+    +    HN +K         
Sbjct: 213 --PQGGILSPLLANIYLHELDKFVLQLKMKFDRESPERITPEYRELHNEIKRISHRLKKL 272

Query: 311 -------------------------------IYAVRYLDEILVITSGSKMLTMELKSQVL 364
                                          +  VRY D+ ++   GSK     +K Q+ 
Sbjct: 273 EGEEKAKVLLEYQEKRKRLPTLPCTSQTNKVLKYVRYADDFIISVKGSKEDCQWIKEQLK 332

BLAST of Lag0018431 vs. ExPASy TrEMBL
Match: A0A6J1ILC2 (nuclear intron maturase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111476629 PE=4 SV=1)

HSP 1 Score: 1339.3 bits (3465), Expect = 0.0e+00
Identity = 681/769 (88.56%), Postives = 717/769 (93.24%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPL----SQHSAQTLTKPQLKALVLSRF 60
           M  HLRKIAT LKPKLS+SLN KLYSD PLKHSPL     QHS QTLT+PQL+ALVLSRF
Sbjct: 1   MLIHLRKIATPLKPKLSSSLN-KLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRF 60

Query: 61  TQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNA--HLPIFDLVSNRFSVQDMSREL 120
           +QGKF DL+QNVVASPSVL TASQNLITPLPSNRLNA   L   D+VS+ FSV+DM+REL
Sbjct: 61  SQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMAREL 120

Query: 121 YENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVG 180
           YENRFDVGACCVR+  SEEKGE LVLPNLKLKVL+EA+++VLEIVYDERFVTFSYGGRVG
Sbjct: 121 YENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAMKMVLEIVYDERFVTFSYGGRVG 180

Query: 181 MGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFE 240
           MGRHTAIRYLKNSV+NPSWWFTVAF RRKFDSVHVNKLCLLVQEKIKDD LI MLKKLFE
Sbjct: 181 MGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFE 240

Query: 241 LGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVS 300
           L A+QIELG  Y+GRGFPQESGLCSIL NIY NGFDKEIQQIRLQKNEENPKF+L+  VS
Sbjct: 241 LEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLNGTVS 300

Query: 301 FHNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEK 360
           FHNPVKIYAVRYLDEILVITSGSKM  MELKSQVL YLEGNLELEVDRMNTAIHSAVSEK
Sbjct: 301 FHNPVKIYAVRYLDEILVITSGSKMQIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEK 360

Query: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420
           ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH
Sbjct: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420

Query: 421 VFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKH 480
           VFKKLK+TDG +SEFQIE EV EIFRNWADEVV+DFFESLEDN EWHR LSAGDFLSLKH
Sbjct: 421 VFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKH 480

Query: 481 IRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLC 540
           IRNQLP  LVNAYDRFQDQV+KHLNP+K KKEKAREDEEKR+ EEE YAKRTVEDLTRLC
Sbjct: 481 IRNQLPVDLVNAYDRFQDQVNKHLNPLKAKKEKAREDEEKRLVEEERYAKRTVEDLTRLC 540

Query: 541 IKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 600
           IKV+APIELVRKAVKM+GFTNKMGRP+PISSLI LEDTDIIKWYAGVGRRWLDFFCCCHN
Sbjct: 541 IKVEAPIELVRKAVKMIGFTNKMGRPQPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHN 600

Query: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKM 660
           YKMVKTIVTYHLRFSCILTLAEKHESTKREA+KHYSKDLKVFDLNG EE+HFPTEREVKM
Sbjct: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKM 660

Query: 661 LGDRNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSN 720
           LG+RNLADPYPVDGA SLFLIRL +DE  YPCIAHFCNRTDSILYRVRLLQ+TLNVNPSN
Sbjct: 661 LGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSN 720

Query: 721 GLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
           G+EWVRGMGVIHESLNQRC+PLCADHISDLYMGKINL+DLDCTLSLDMD
Sbjct: 721 GVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Lag0018431 vs. ExPASy TrEMBL
Match: A0A6J1FV64 (nuclear intron maturase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111447574 PE=4 SV=1)

HSP 1 Score: 1335.5 bits (3455), Expect = 0.0e+00
Identity = 680/769 (88.43%), Postives = 715/769 (92.98%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPL----SQHSAQTLTKPQLKALVLSRF 60
           M  HLRKIAT LKPKLS+S N KLYSD PLKHSPL     QHS QTLT+PQL+ALVLSRF
Sbjct: 1   MLIHLRKIATPLKPKLSSSFN-KLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRF 60

Query: 61  TQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNA--HLPIFDLVSNRFSVQDMSREL 120
           +QGKF DL+QNVVASPSVL TASQNLITPLPSNRLNA   L   D+VS+ FSV+DM+REL
Sbjct: 61  SQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSLDMVSSCFSVEDMAREL 120

Query: 121 YENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVG 180
           YENRFDVGACCVR+  SEEKGE LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVG
Sbjct: 121 YENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVG 180

Query: 181 MGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFE 240
           MGRHTAIRYLKNSV+NPSWWFTVAF RRKFDSVHVNKLCLLVQEKIKDD LI MLKKLFE
Sbjct: 181 MGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFE 240

Query: 241 LGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVS 300
           L A+QIELG  Y+GRG PQESGLCSIL NIY NGFDKEIQQIRL+KNEENPKF+LD  VS
Sbjct: 241 LEAVQIELGGCYLGRGLPQESGLCSILSNIYFNGFDKEIQQIRLEKNEENPKFSLDGTVS 300

Query: 301 FHNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEK 360
           FHNPVKIYAVRYLDEILVITSGSKML MELKSQVL YLEGNLELEVDRMNTAIHSAVSEK
Sbjct: 301 FHNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEK 360

Query: 361 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420
           ISFLGMELQAV PSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH
Sbjct: 361 ISFLGMELQAVLPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH 420

Query: 421 VFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKH 480
           VFKKLK+TDG +SEFQIE EV EIFRNWADEVV+DFFESLEDN EWHR LSAGDFLSLKH
Sbjct: 421 VFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKH 480

Query: 481 IRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLC 540
           IRNQLP  LVNAYDRFQDQV++HLNP+K K+EKAREDEEKR+ EEE YAKRTVEDLTRLC
Sbjct: 481 IRNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLC 540

Query: 541 IKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 600
           IKV+APIELVRKAVKM+GFTNKMGRPRPISSLI LEDTDIIKWYAGVGRRWLDFFCCCHN
Sbjct: 541 IKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHN 600

Query: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKM 660
           YKMVKTIVTYHLRFSCILTLAEKHESTKREA+KHYSKDLKVFDLNG EE+HFPTEREVKM
Sbjct: 601 YKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKM 660

Query: 661 LGDRNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSN 720
           LG+RNLADPYPVDGA SLFLIRL +DE  YPCIAHFCNRTDSILYRVRLLQ+TLNVNPSN
Sbjct: 661 LGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSN 720

Query: 721 GLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
           G+EWVRGMGVIHESLNQRC+PLCADHISDLYMGKINL+DLDCTLSLDMD
Sbjct: 721 GVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Lag0018431 vs. ExPASy TrEMBL
Match: A0A1S4E4Y1 (uncharacterized protein LOC103501588 OS=Cucumis melo OX=3656 GN=LOC103501588 PE=4 SV=1)

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 668/766 (87.21%), Postives = 710/766 (92.69%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPL---SQHSAQTLTKPQLKALVLSRFT 60
           MF H RKIAT LKPK S SLN KLYS LPL+   L   SQHS ++LT  QLKALVLSRF+
Sbjct: 1   MFIHFRKIATSLKPKFSNSLN-KLYSHLPLRKKVLNLPSQHSPESLTSSQLKALVLSRFS 60

Query: 61  QGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNAHLPIFDLVSNRFSVQDMSRELYEN 120
            GKF+DL QNVVASPSVLLTAS+NLITP  SN  ++ LP+ DLVS  FSV+ M+REL EN
Sbjct: 61  HGKFVDLFQNVVASPSVLLTASRNLITPPFSNAPDS-LPLSDLVSKCFSVEVMARELSEN 120

Query: 121 RFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVGMGR 180
           RFDVGACCV M P EEKGESLVLPNLKLKVLIEAIR+VLEIVYDERFVTFSYGGRVGMGR
Sbjct: 121 RFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGR 180

Query: 181 HTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFELGA 240
           HTAIRYLKNSV+NPSWWFTVAF R+KF+SVHVN LCLL+QEKIKDD LIYML+KLFE+ A
Sbjct: 181 HTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVEA 240

Query: 241 IQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVSFHN 300
           IQIELG  Y+GRGFPQESGLCSIL NIY NGFDKEIQ+IRLQKNEENPKFNLD+IVSFHN
Sbjct: 241 IQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSFHN 300

Query: 301 PVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEKISF 360
           PVKIYAVRYLDEILVITSGSKMLTMELKSQVL YLEGNLELEVDRMNTAIHSAVSEKI F
Sbjct: 301 PVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIGF 360

Query: 361 LGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFK 420
           LGMELQAV PSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH+FK
Sbjct: 361 LGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHMFK 420

Query: 421 KLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKHIRN 480
           K K+T GF+SEFQIE EVR IFRNWADEVVQDFFES ED+AEWHRVLSAGDFLSLKHIRN
Sbjct: 421 KFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDHAEWHRVLSAGDFLSLKHIRN 480

Query: 481 QLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLCIKV 540
           QLPE LVNAYDRFQ QV+KHLNPVKVKKEKAREDEEKR+EEEE YAKRTVEDLTRLCIKV
Sbjct: 481 QLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCIKV 540

Query: 541 DAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHNYKM 600
           DAPIELVRKAV+MVGFTNKMGRPRPISSLI LED DIIKWY+GVGRRWLDFFCCCHNYKM
Sbjct: 541 DAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNYKM 600

Query: 601 VKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKMLGD 660
           VKT+VTYHLRFSCILTLAEKHESTKREA+KHY KDLKVFDLNGNEE+HFPTE+ VKMLG+
Sbjct: 601 VKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKMLGE 660

Query: 661 RNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGLE 720
           RNLADPYPVDGALSL LIRLA+DEA YPCIA+FCNRT+SILYRVRLLQRTLNVNPS+G+E
Sbjct: 661 RNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGVE 720

Query: 721 WVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
           WV+GMGVIHESLNQRC+PLCADHISDLYMGKINL+DLDCTLSLDMD
Sbjct: 721 WVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

BLAST of Lag0018431 vs. ExPASy TrEMBL
Match: A0A5A7SWY5 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold400G00320 PE=4 SV=1)

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 669/766 (87.34%), Postives = 709/766 (92.56%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSPL---SQHSAQTLTKPQLKALVLSRFT 60
           MF H RKIAT LKPK S SLN KLYS LPL+   L   SQHS ++LT  QLKALVLSRF+
Sbjct: 1   MFIHFRKIATSLKPKFSNSLN-KLYSHLPLRKKVLNLPSQHSPESLTSSQLKALVLSRFS 60

Query: 61  QGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNAHLPIFDLVSNRFSVQDMSRELYEN 120
            GKF+DL QNVVASPSVLLTAS+NLITP  SN  ++ LP+ DLVS  FSV+ M+REL EN
Sbjct: 61  HGKFVDLFQNVVASPSVLLTASRNLITPPFSNAPDS-LPLSDLVSKCFSVEVMARELSEN 120

Query: 121 RFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGRVGMGR 180
           RFDVGACCV M P EEKGESLVLPNLKLKVLIEAIR+VLEIVYDERFVTFSYGGRVGMGR
Sbjct: 121 RFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGR 180

Query: 181 HTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKLFELGA 240
           HTAIRYLKNSV+NPSWWFTVAF R+KF+SVHVN LCLL+QEKIKDD LIYML+KLFE+ A
Sbjct: 181 HTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEVEA 240

Query: 241 IQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDIVSFHN 300
           IQIELG  Y+GRGFPQESGLCSIL NIY NGFDKEIQ+IRLQKNEENPKFNLD+IVSFHN
Sbjct: 241 IQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSFHN 300

Query: 301 PVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEKISF 360
           PVKIYAVRYLDEILVITSGSKMLTMELKSQVL YLEGNLELEVDRMNTAIHSAVSEKI F
Sbjct: 301 PVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKIGF 360

Query: 361 LGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFK 420
           LGMELQAV PSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFK
Sbjct: 361 LGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFK 420

Query: 421 KLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKHIRN 480
           K K+T GF+SEFQIE EVR IFRNWADEVVQDFFES ED AEWHRVLSAGDFLSLKHIRN
Sbjct: 421 KFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDPAEWHRVLSAGDFLSLKHIRN 480

Query: 481 QLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLCIKV 540
           QLPE LVNAYDRFQ QV+KHLNPVKVKKEKAREDEEKR+EEEE YAKRTVEDLTRLCIKV
Sbjct: 481 QLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCIKV 540

Query: 541 DAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHNYKM 600
           DAPIELVRKAV+MVGFTNKMGRPRPISSLI LED DIIKWY+GVGRRWLDFFCCCHNYKM
Sbjct: 541 DAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNYKM 600

Query: 601 VKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKMLGD 660
           VKT+VTYHLRFSCILTLAEKHESTKREA+KHY KDLKVFDLNGNEE+HFPTE+ VKMLG+
Sbjct: 601 VKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKMLGE 660

Query: 661 RNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGLE 720
           RNLADPYPVDGALSL LIRLA+DEA YPCIA+FCNRT+SILYRVRLLQRTLNVNPS+G+E
Sbjct: 661 RNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDGVE 720

Query: 721 WVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
           WV+GMGVIHESLNQRC+PLCADHISDLYMGKINL+DLDCTLSLDMD
Sbjct: 721 WVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

BLAST of Lag0018431 vs. ExPASy TrEMBL
Match: A0A6J1DNH6 (nuclear intron maturase 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111022853 PE=4 SV=1)

HSP 1 Score: 1300.0 bits (3363), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 711/771 (92.22%), Query Frame = 0

Query: 1   MFFHLRKIATLLKPKLSTSLNSKLYSDLPLKHSP-----LSQHSAQTLTKPQLKALVLSR 60
           MF  LRKIAT LK  LSTS N KLYSDLPLKHSP       QHS +TLT P+LKALVLSR
Sbjct: 1   MFIPLRKIATPLKSILSTSFN-KLYSDLPLKHSPPLHNFPFQHSTETLTWPELKALVLSR 60

Query: 61  FTQGKFLDLIQNVVASPSVLLTASQNLIT-PLPSNRLNA--HLPIFDLVSNRFSVQDMSR 120
           FT GKFLDL+QNVVASPSVLLTASQNLIT P PSN LNA   LPI DLVS  FSV++M+R
Sbjct: 61  FTHGKFLDLLQNVVASPSVLLTASQNLITPPPPSNGLNAPDPLPILDLVSKCFSVEEMAR 120

Query: 121 ELYENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIRLVLEIVYDERFVTFSYGGR 180
           ELYE+RFDVGACCVRM   ++KG+SLVLPNLKLKVLIEAIR+VLEIVYDERFVTFSYGGR
Sbjct: 121 ELYEDRFDVGACCVRM---DQKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGR 180

Query: 181 VGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLCLLVQEKIKDDTLIYMLKKL 240
           VGMGRHTAIRYLKNSV+NPSWWFTVAF R+KFDSVHV+KLCLLV+EKIKD  LI MLKKL
Sbjct: 181 VGMGRHTAIRYLKNSVQNPSWWFTVAFRRKKFDSVHVHKLCLLVEEKIKDHLLICMLKKL 240

Query: 241 FELGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEIQQIRLQKNEENPKFNLDDI 300
           FEL AIQIELGA Y+GRGFPQESGLCSIL NI+ NGFDK+IQQIRLQKNEENPKF+LD+I
Sbjct: 241 FELEAIQIELGACYLGRGFPQESGLCSILCNIFFNGFDKDIQQIRLQKNEENPKFSLDEI 300

Query: 301 VSFHNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVS 360
           VSFH+PVKIYAVRYLDEILVITSGSKMLTM+LKSQVL YLEGNLELEVDRMNTAIHSAVS
Sbjct: 301 VSFHSPVKIYAVRYLDEILVITSGSKMLTMDLKSQVLKYLEGNLELEVDRMNTAIHSAVS 360

Query: 361 EKISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKIL 420
           EKISFLGMELQAVPPSVLHPPMSEKAIRARKKY+RQKEVR IELRNARERNRKKLGLKIL
Sbjct: 361 EKISFLGMELQAVPPSVLHPPMSEKAIRARKKYIRQKEVRVIELRNARERNRKKLGLKIL 420

Query: 421 SHVFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSL 480
           SHVFKKLKQTDGF+ EFQIE EVREIFRNWADEV Q FFESLE++AEWH  LSAGDFLSL
Sbjct: 421 SHVFKKLKQTDGFKFEFQIEKEVREIFRNWADEVAQHFFESLENHAEWHHALSAGDFLSL 480

Query: 481 KHIRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTR 540
           KHIRNQLPE LVNAYDRFQDQVDKHLNPVKVK  KAREDEEKRVEEE++YA+RTVEDLTR
Sbjct: 481 KHIRNQLPEDLVNAYDRFQDQVDKHLNPVKVKYVKAREDEEKRVEEEQKYARRTVEDLTR 540

Query: 541 LCIKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCC 600
           LCIKVDAPIELVRKAVKMVGFTNKMGRP+PIS L+ LED DIIKWYAGVGRRWLDFFCCC
Sbjct: 541 LCIKVDAPIELVRKAVKMVGFTNKMGRPQPISLLVALEDIDIIKWYAGVGRRWLDFFCCC 600

Query: 601 HNYKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREV 660
           HNYKMVKT+VTYHLRFSCILTLAEKHESTKREA+KH+SKDLKVFDLNG+EE+HFPTEREV
Sbjct: 601 HNYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKHFSKDLKVFDLNGDEEIHFPTEREV 660

Query: 661 KMLGDRNLADPYPVDGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNP 720
           KMLGDR LADPYPVDG LSLFLIRLA DE   PCIAHFCNRTDSILYRVRLLQRTLNVN 
Sbjct: 661 KMLGDRMLADPYPVDGTLSLFLIRLAIDEPSCPCIAHFCNRTDSILYRVRLLQRTLNVNS 720

Query: 721 SNGLEWVRGMGVIHESLNQRCVPLCADHISDLYMGKINLEDLDCTLSLDMD 764
            +G +WVRGMGVIHESLNQRC+PLCADHISDLYMG+INL+DLDCTLSLDMD
Sbjct: 721 CDGEKWVRGMGVIHESLNQRCLPLCADHISDLYMGRINLQDLDCTLSLDMD 767

BLAST of Lag0018431 vs. TAIR 10
Match: AT5G04050.2 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 739.6 bits (1908), Expect = 2.6e-213
Identity = 407/740 (55.00%), Postives = 504/740 (68.11%), Query Frame = 0

Query: 33  SPLSQHSAQTLTKP----QLKALVLSRFTQGKFLDLIQNVVASPSVLLTASQNLITPLPS 92
           S L  +S QT+T+P    +L+ALVL +++ GKF  L++N V+ P VLL A QNL     S
Sbjct: 30  SSLFLNSDQTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNL-----S 89

Query: 93  NRLNAHLPIFDLVSNRFSVQDMSRELYENRFDVGACCVRMLPSEEKGESLVLPNLKLKVL 152
              N+   + D VS RFS+++M RE+ E RFD+ +CCV  + S     SLVLPNLKLKVL
Sbjct: 90  LSANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISS-----SLVLPNLKLKVL 149

Query: 153 IEAIRLVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVH 212
           IEAIR+VLEIVYD+RF TFSYGGRVGMGRHTAIRYLKNSVENP WWF V+F R  F+  +
Sbjct: 150 IEAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERN 209

Query: 213 VNKLCLLVQEKIKDDTLIYMLKKLFELGAIQIELGASYMGRGFPQESGLCSILFNIYLNG 272
           V+ LC  V EKI D  LI M+KKLFE G ++IELG    GRGFPQE GLCSIL N+Y +G
Sbjct: 210 VDILCGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDG 269

Query: 273 FDKEIQQIRLQKNEENPKFNLDDIVS----FHNPVKIYAVRYLDEILVITSGSKMLTMEL 332
            DKEIQ +RL+   +NP+    D  S    F  PV IYAVRYLDEILVITSGSKMLTM+L
Sbjct: 270 LDKEIQDLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDL 329

Query: 333 KSQVLMYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRARKK 392
           K +++  LE  LEL VDR+NT+IHSAVSEKI+FLGM LQAVPPSVL PP SEKA+RA KK
Sbjct: 330 KKRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKK 389

Query: 393 YLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTDGFRSEFQIENEVREIFRNWAD 452
           Y RQK+VR +ELRNARERNRK LGLKI  HV KK+KQ++GF+ E +IENEVR+IF++W +
Sbjct: 390 YQRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGE 449

Query: 453 EVVQDFFESLEDNAEWHRVLSAGDFLSLKHIRNQLPEGLVNAYDRFQDQVDKHLNPVKVK 512
           EV+QDF  SLE+  +WH +L+ GDFLSL+HIR +LP+ L++AYD FQ+QVDKHL P + K
Sbjct: 450 EVMQDFMGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK 509

Query: 513 KEKAREDEEKRVEEEEE--YAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPRP 572
             K  EDEE+RVEEEEE  YA+RTVEDLT+LC+KV AP ELVRKA+              
Sbjct: 510 --KVLEDEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAI-------------- 569

Query: 573 ISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSCILTLAEKHESTK 632
                                                                       
Sbjct: 570 ------------------------------------------------------------ 629

Query: 633 REAIKHYSKDLKVFDLNGNEELHFPTEREVKMLGDRNLADPYPVDGALSLFLIRLASDEA 692
                      KV DL+G EE HFP+EREVKM+GD+NL+DP PVDG LSL LIRLASDE 
Sbjct: 630 -----------KVSDLDGREEAHFPSEREVKMMGDKNLSDPKPVDGTLSLLLIRLASDEP 672

Query: 693 PYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGLEWVRGMGVIHESLNQRCVPLCADHIS 752
            + C A FC R+D+I++RV LLQ  L++NP +  +WV GMG IH +LN++C+PLC+ HIS
Sbjct: 690 LHHCAASFCERSDTIMHRVHLLQNRLHINPLDEEKWVPGMGTIHSALNRKCLPLCSTHIS 672

Query: 753 DLYMGKINLEDLDCTLSLDM 763
           D+Y+GKI L+D+D +  +D+
Sbjct: 750 DVYLGKITLQDVDSSSFIDL 672

BLAST of Lag0018431 vs. TAIR 10
Match: AT5G04050.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 710.7 bits (1833), Expect = 1.3e-204
Identity = 374/580 (64.48%), Postives = 451/580 (77.76%), Query Frame = 0

Query: 33  SPLSQHSAQTLTKP----QLKALVLSRFTQGKFLDLIQNVVASPSVLLTASQNLITPLPS 92
           S L  +S QT+T+P    +L+ALVL +++ GKF  L++N V+ P VLL A QNL     S
Sbjct: 30  SSLFLNSDQTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNL-----S 89

Query: 93  NRLNAHLPIFDLVSNRFSVQDMSRELYENRFDVGACCVRMLPSEEKGESLVLPNLKLKVL 152
              N+   + D VS RFS+++M RE+ E RFD+ +CCV  + S     SLVLPNLKLKVL
Sbjct: 90  LSANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISS-----SLVLPNLKLKVL 149

Query: 153 IEAIRLVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVH 212
           IEAIR+VLEIVYD+RF TFSYGGRVGMGRHTAIRYLKNSVENP WWF V+F R  F+  +
Sbjct: 150 IEAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERN 209

Query: 213 VNKLCLLVQEKIKDDTLIYMLKKLFELGAIQIELGASYMGRGFPQESGLCSILFNIYLNG 272
           V+ LC  V EKI D  LI M+KKLFE G ++IELG    GRGFPQE GLCSIL N+Y +G
Sbjct: 210 VDILCGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDG 269

Query: 273 FDKEIQQIRLQKNEENPKFNLDDIVS----FHNPVKIYAVRYLDEILVITSGSKMLTMEL 332
            DKEIQ +RL+   +NP+    D  S    F  PV IYAVRYLDEILVITSGSKMLTM+L
Sbjct: 270 LDKEIQDLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDL 329

Query: 333 KSQVLMYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRARKK 392
           K +++  LE  LEL VDR+NT+IHSAVSEKI+FLGM LQAVPPSVL PP SEKA+RA KK
Sbjct: 330 KKRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKK 389

Query: 393 YLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTDGFRSEFQIENEVREIFRNWAD 452
           Y RQK+VR +ELRNARERNRK LGLKI  HV KK+KQ++GF+ E +IENEVR+IF++W +
Sbjct: 390 YQRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGE 449

Query: 453 EVVQDFFESLEDNAEWHRVLSAGDFLSLKHIRNQLPEGLVNAYDRFQDQVDKHLNPVKVK 512
           EV+QDF  SLE+  +WH +L+ GDFLSL+HIR +LP+ L++AYD FQ+QVDKHL P + K
Sbjct: 450 EVMQDFMGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK 509

Query: 513 KEKAREDEEKRVEEEEE--YAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPRP 572
             K  EDEE+RVEEEEE  YA+RTVEDLT+LC+KV AP ELVRKA+K+VGFTN MGRPRP
Sbjct: 510 --KVLEDEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAIKLVGFTNSMGRPRP 569

Query: 573 ISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIV 603
           I  L+ LED+DIIKWYAGVGR+WLDFFCCCHNYKMVK++V
Sbjct: 570 IIHLVTLEDSDIIKWYAGVGRKWLDFFCCCHNYKMVKSLV 597

BLAST of Lag0018431 vs. TAIR 10
Match: AT1G74350.1 (Intron maturase, type II family protein )

HSP 1 Score: 226.5 bits (576), Expect = 7.3e-59
Identity = 194/764 (25.39%), Postives = 346/764 (45.29%), Query Frame = 0

Query: 34  PLSQHSAQTLTKPQLKALVLSRFTQGKFLDLIQNVVASPSVLLTASQNLITPLPSNRLNA 93
           P S+   +   + +LK  V  +   GKF DL++ V+A P  L  A   +       RLN+
Sbjct: 32  PRSRMELKRSLELRLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCI-------RLNS 91

Query: 94  HLPIFDLVSNRFSVQDMSRELYENRFDVGACCVRMLPSEEKGESLVLPNLKLKVLIEAIR 153
           ++ I +  +   +   ++ EL    FDV +    ++  ++  E LVLP++ LKV+ EAIR
Sbjct: 92  NVSITER-NGSVAFDSIAEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIR 151

Query: 154 LVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFDSVHVNKLC 213
           +VLE+V+   F   S+  R G GR +A++Y+ N++    W FT++ +++   SV  N L 
Sbjct: 152 IVLEVVFSPHFSKISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLS 211

Query: 214 LLVQEKIKDDTLIYMLKKLFELGAIQIELGASYMGRGFPQESGLCSILFNIYLNGFDKEI 273
           ++ +EK++D +L  +L+ +FE   + +E G    G G PQE  L  +L NIYL+ FD E 
Sbjct: 212 VM-EEKVEDSSLSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEF 271

Query: 274 QQIRL---------QKNEENP-------------KFNLDDIVSFHNPVKIYAVRYLDEIL 333
            +I +         + +E++P             +  L         +++Y  R++DEI 
Sbjct: 272 YRISMRHEALGLDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVALRVYCCRFMDEIY 331

Query: 334 VITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLH 393
              SG K +  +++S+ + +L  +L L++           +  +  LG     V  +V  
Sbjct: 332 FSVSGPKKVASDIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG---TLVRKNVRE 391

Query: 394 PPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTD-------- 453
            P + KA+   K+ +R   + A++   A      ++G K L H  KK+K+++        
Sbjct: 392 SP-TVKAVHKLKEKVR---LFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKGLADSN 451

Query: 454 --------GFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLKH- 513
                     ++  + ++  + + R W ++V++    +  D +E        +F+  KH 
Sbjct: 452 STLSQISCHRKAGMETDHWYKILLRIWMEDVLR----TSADRSE--------EFVLSKHV 511

Query: 514 IRNQLPEGLVNAYDRFQDQVDKHLNPVKVKKEKAREDEEKRVEEEEEYAKRTVEDLTRLC 573
           +   +P+ L +A+ +FQ+    +++      E      +               D     
Sbjct: 512 VEPTVPQELRDAFYKFQNAAAAYVSSETANLEALLPCPQS-------------HDRPVFF 571

Query: 574 IKVDAPIELVRKAVKMVGFTNKMGRPRPISSLIVLEDTDIIKWYAGVGRRWLDFFCCCHN 633
             V AP   + + +   G     G  R  S LI+L+   II WY+G+ RRW+ ++  C N
Sbjct: 572 GDVVAPTNAIGRRLYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSN 631

Query: 634 YKMVKTIVTYHLRFSCILTLAEKHESTKREAIKHYSKDLKVFDLNGNEELHFPTEREVKM 693
           +  +K ++   +R SCI TLA K+   + E  K    +L       + E     E+    
Sbjct: 632 FDEIKALIDNQIRMSCIRTLAAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSP 691

Query: 694 LGDRNLADPYPV--DGALSLFLIRLASDEAPYPCIAHFCNRTDSILYRVRLLQRTLNVNP 753
             DR+    Y +   G   L L RL S+  P  C    C+     +Y +  ++R      
Sbjct: 692 AFDRDEHLTYGLSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------ 748

Query: 754 SNGLEWVRGMGV-IHESLNQRCVPLCADHISDLYMGKINLEDLD 756
                W  G  V I  SLN R + LC  H+ DLY+G+I+L+ +D
Sbjct: 752 QKFPGWKTGFSVCIPSSLNGRRIGLCKQHLKDLYIGQISLQAVD 748

BLAST of Lag0018431 vs. TAIR 10
Match: ATMG00520.1 (Intron maturase, type II family protein )

HSP 1 Score: 78.2 bits (191), Expect = 3.2e-14
Identity = 145/676 (21.45%), Postives = 244/676 (36.09%), Query Frame = 0

Query: 146 KVLIEAIRLVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVENPSWWFTVAFHRRKFD 205
           K++ EAIR+VLE +YD  F   S+  R G G H+ +R +K      S WF     R+ F 
Sbjct: 15  KIMKEAIRMVLESIYDPEFPDTSH-FRSGQGCHSVLRRIKEE-WGISRWFLEFDIRKCFH 74

Query: 206 SVHVNKLCLLVQEKIKDDTLIYMLKKLFELGAIQIELGASYMGRGFPQESGLCSILFNIY 265
           ++  ++L  +++E+I D    Y ++K+F  G +   +G        P    L ++  NIY
Sbjct: 75  TIDRHRLIQILKEEIDDPKFFYSIQKVFSAGRL---VGVERGPYSVPHSVLLSALPGNIY 134

Query: 266 LNGFDKEIQQIRLQ--------------------KNEENP----KFNLDD---------- 325
           L+  D+EI +IR +                     ++ENP     FN             
Sbjct: 135 LHKLDQEIGRIRQKYEIPIVQRVRSVLLRTGRRIDDQENPGEEASFNAPQDNRAIIVGSV 194

Query: 326 ------------IVSFHNP----------------------------------------- 385
                       + S+H P                                         
Sbjct: 195 KSMQRKAAFHSLVSSWHTPPTSTLRLRGDQKRPFVFPPSSALAVFLNKPSSLLCAAFLIE 254

Query: 386 ------------------------------------------------------------ 445
                                                                       
Sbjct: 255 AAGLTPKAEFYGGERCNNNWAMRDLLKYCKRKGLLIELGGEAILVIRSERGLARKQAPLK 314

Query: 446 ----VKIYAVRYLDEILVITSGSKMLTMELKSQVLMYLEGNLELEVDRMNTAIHSAVSEK 505
               ++I   RY D++L+   G+  L +E++ ++  +L+  L L V    +   +A S  
Sbjct: 315 THYLIRICYARYADDLLLGIVGAVELLIEIQKRIAHFLQSGLNLWVGSAGSTTIAARS-T 374

Query: 506 ISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKI-LS 565
           + FLG  ++ VPP    P    + +  R +   +  + A  LR+A     + LG  I + 
Sbjct: 375 VEFLGTVIREVPPRTT-PIQFLRELEKRLRVKHRIHITACHLRSAIHSKFRNLGDSIPIK 434

Query: 566 HVFKKLKQTDGFRSEFQIENEVREIFRNWADEVVQDFFESLEDNAEWHRVLSAGDFLSLK 625
            + K + +T   +   Q+           A+ +      S + +  W  V         K
Sbjct: 435 QLTKGMSKTGSLQDGVQL-----------AETLGTAGVRSPQVSVLWGTV---------K 494

Query: 626 HIRNQL-------PEGLVNAYDRFQDQVDK---HLNPVKVKKEKAREDEEKRVEEEEEYA 657
           HIR            G  NA    Q  V +   H   + +     R    K   E   + 
Sbjct: 495 HIRQGSRGISFLHSSGRSNASSDVQQVVSRSGTHARKLSLYTPPGR----KAAGEGGGHW 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6600779.10.0e+0088.82Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp.... [more]
KAG7031416.10.0e+0088.82Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp.... [more]
XP_022976163.10.0e+0088.56nuclear intron maturase 3, mitochondrial [Cucurbita maxima][more]
XP_022942578.10.0e+0088.43nuclear intron maturase 3, mitochondrial [Cucurbita moschata][more]
XP_023544815.10.0e+0087.78nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544... [more]
Match NameE-valueIdentityDescription
Q9LZA57.0e-24059.73Nuclear intron maturase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT... [more]
Q9CA781.0e-5725.39Nuclear intron maturase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT... [more]
P055117.0e-1427.78Uncharacterized 91 kDa protein in cob intron OS=Schizosaccharomyces pombe (strai... [more]
P038769.1e-1424.40Putative COX1/OXI3 intron 2 protein OS=Saccharomyces cerevisiae (strain ATCC 204... [more]
P0A3U05.9e-1324.36Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris OX=13... [more]
Match NameE-valueIdentityDescription
A0A6J1ILC20.0e+0088.56nuclear intron maturase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A6J1FV640.0e+0088.43nuclear intron maturase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A1S4E4Y10.0e+0087.21uncharacterized protein LOC103501588 OS=Cucumis melo OX=3656 GN=LOC103501588 PE=... [more]
A0A5A7SWY50.0e+0087.34RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1DNH60.0e+0086.90nuclear intron maturase 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT5G04050.22.6e-21355.00RNA-directed DNA polymerase (reverse transcriptase) [more]
AT5G04050.11.3e-20464.48RNA-directed DNA polymerase (reverse transcriptase) [more]
AT1G74350.17.3e-5925.39Intron maturase, type II family protein [more]
ATMG00520.13.2e-1421.45Intron maturase, type II family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 501..529
NoneNo IPR availablePANTHERPTHR33642:SF4NUCLEAR INTRON MATURASE 3, MITOCHONDRIALcoord: 17..759
NoneNo IPR availablePANTHERPTHR33642COX1/OXI3 INTRON 1 PROTEIN-RELATEDcoord: 17..759
NoneNo IPR availableCDDcd01651RT_G2_introncoord: 137..362
e-value: 1.02653E-38
score: 141.57
IPR024937Domain XPFAMPF01348Intron_maturas2coord: 537..640
e-value: 1.2E-15
score: 57.7
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 139..361
e-value: 3.2E-10
score: 39.9
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 110..362
score: 10.597533

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018431.1Lag0018431.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006315 homing of group II introns
biological_process GO:0090615 mitochondrial mRNA processing
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
biological_process GO:0006397 mRNA processing
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003964 RNA-directed DNA polymerase activity