Lag0017391 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017391
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	protein N-lysine methyltransferase METTL21A-like
Location	chr5: 3084690 .. 3085364 (-)
RNA-Seq Expression	Lag0017391
Synteny	Lag0017391

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGTTAGAGAAATCCAAGTCAGTGATAGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGTACAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTGGTCCTAGCCGAATGGATGGCAACTCGGAGCAAGCTACTCGACTTCGACTTCCGGGACAAGACTGTGATTGAGCTCGGCGCTGGGGCTGGACTCCCAGGTTTGATGGCAGCTCTTCTTGGTGCCAGTCGTGTTGTACTTACTGATGTAGAGCCATTGCTTCCTGGGTTGTTGAAGAATGCAGATGCCAATGGACTTGGGGATCGAGTGGAAGTAAGGGAACTCGTCTGGGGTTTGGATAAACCGGTGAGTGAAGTGAACAAGTTGGGGGAGTTTGACCTAATATTGATGTCGGATTTGTTCTATGACTCGGAGGAAATGGCCCCTCTAGCACACATCCTAAAGACCATCTCAGGAAAAGATAGCAAAATCTGGGCAGCTTCCGAGATCCGACCATGGACAGTCGAGTGTCTAACCGAGTTCATGAATCAAGGATTCAAGATCGAAGAGACACCAGTTCAACTTGACCAACCCTGTGAGGAAGATGACTATGCCATCTTAGAATCATTTTCTATTTTCCATCTCCAACCACCAGATGAAGAAGAAAGCAACTTTTAA

mRNA sequence

ATGGGTGTTAGAGAAATCCAAGTCAGTGATAGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGTACAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTGGTCCTAGCCGAATGGATGGCAACTCGGAGCAAGCTACTCGACTTCGACTTCCGGGACAAGACTGTGATTGAGCTCGGCGCTGGGGCTGGACTCCCAGGTTTGATGGCAGCTCTTCTTGGTGCCAGTCGTGTTGTACTTACTGATGTAGAGCCATTGCTTCCTGGGTTGTTGAAGAATGCAGATGCCAATGGACTTGGGGATCGAGTGGAAGTAAGGGAACTCGTCTGGGGTTTGGATAAACCGGTGAGTGAAGTGAACAAGTTGGGGGAGTTTGACCTAATATTGATGTCGGATTTGTTCTATGACTCGGAGGAAATGGCCCCTCTAGCACACATCCTAAAGACCATCTCAGGAAAAGATAGCAAAATCTGGGCAGCTTCCGAGATCCGACCATGGACAGTCGAGTGTCTAACCGAGTTCATGAATCAAGGATTCAAGATCGAAGAGACACCAGTTCAACTTGACCAACCCTGTGAGGAAGATGACTATGCCATCTTAGAATCATTTTCTATTTTCCATCTCCAACCACCAGATGAAGAAGAAAGCAACTTTTAA

Coding sequence (CDS)

ATGGGTGTTAGAGAAATCCAAGTCAGTGATAGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGTACAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTGGTCCTAGCCGAATGGATGGCAACTCGGAGCAAGCTACTCGACTTCGACTTCCGGGACAAGACTGTGATTGAGCTCGGCGCTGGGGCTGGACTCCCAGGTTTGATGGCAGCTCTTCTTGGTGCCAGTCGTGTTGTACTTACTGATGTAGAGCCATTGCTTCCTGGGTTGTTGAAGAATGCAGATGCCAATGGACTTGGGGATCGAGTGGAAGTAAGGGAACTCGTCTGGGGTTTGGATAAACCGGTGAGTGAAGTGAACAAGTTGGGGGAGTTTGACCTAATATTGATGTCGGATTTGTTCTATGACTCGGAGGAAATGGCCCCTCTAGCACACATCCTAAAGACCATCTCAGGAAAAGATAGCAAAATCTGGGCAGCTTCCGAGATCCGACCATGGACAGTCGAGTGTCTAACCGAGTTCATGAATCAAGGATTCAAGATCGAAGAGACACCAGTTCAACTTGACCAACCCTGTGAGGAAGATGACTATGCCATCTTAGAATCATTTTCTATTTTCCATCTCCAACCACCAGATGAAGAAGAAAGCAACTTTTAA

Protein sequence

MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDKTVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDKPVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEFMNQGFKIEETPVQLDQPCEEDDYAILESFSIFHLQPPDEEESNF

Homology

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BLAST of Lag0017391 vs. NCBI nr
Match: KAG7015681.1 (EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 191/223 (85.65%), Postives = 205/223 (91.93%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           MGVRE Q+SD N+VIHELDDLYDSTTG VLTGSWVWNSA VLAEWMATR KL DFDFRDK
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATRCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
           TVIELGAGAGLPGLMAALLGA RVVLTDVEPLLPGLL+N DANGL DRVEVRELVWGLD+
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
            VS  N+ GEFDLILMSDLFYDSE+MAPLAHILK ISGK+SKIWAASEIRPWTVECLTEF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 MNQGFKIEETPVQLDQPCEE-DDYAILESFSIFHLQPPDEEES 223
           MN+GFK+EE+ VQLD+P EE DD+AIL+SFSIFHLQPPDEE +
Sbjct: 181 MNEGFKVEESQVQLDEPREEVDDHAILDSFSIFHLQPPDEERN 223

[Close]

BLAST of Lag0017391 vs. NCBI nr
Match: XP_022923423.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata])

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 190/224 (84.82%), Postives = 206/224 (91.96%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           MGVRE Q+SD N+VIHELDDLYDSTTG VLTGSWVWNSA VLAEWMAT+ KL DFDFRDK
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
           TVIELGAGAGLPGLMAALLGA RVVLTDVEPLLPGLL+N DANGL DRVEVRELVWGLD+
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
            VS  N+ GEFDLILMSDLFYDSE+MAPLAHILK ISGK+SKIWAASEIRPWTVECLTEF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 MNQGFKIEETPVQLDQPCEE-DDYAILESFSIFHLQPPDEEESN 224
           MN+GFK+EE+ VQLD+P EE DD+AIL+SFSIFHLQPPDEE ++
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERNS 224

[Close]

BLAST of Lag0017391 vs. NCBI nr
Match: XP_023553140.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387.1 bits (993), Expect = 1.0e-103
Identity = 189/224 (84.38%), Postives = 204/224 (91.07%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           MGVRE Q+SDRN+VIHELDDLYDSTTG VLTGSWVWNSA VLAEWMAT+ KL DFDFRDK
Sbjct: 24  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 83

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
           TVIELGAGAGLPGLMAALLGA RVVLTDVEPLLPGLL+N DANGL DRVEVRELVWGLD+
Sbjct: 84  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 143

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
            VS  N+ GEFDLILMSDLFYDSE+MAPLAHILK ISGK SKIWAASEIRPWTVECLTEF
Sbjct: 144 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKHSKIWAASEIRPWTVECLTEF 203

Query: 181 MNQGFKIEETPVQLDQPCEE-DDYAILESFSIFHLQPPDEEESN 224
            N+GFK+EE+ VQLD+P EE DD+ IL+SFSIFHLQPPDEE ++
Sbjct: 204 TNEGFKVEESQVQLDEPREEDDDHTILDSFSIFHLQPPDEERNS 247

[Close]

BLAST of Lag0017391 vs. NCBI nr
Match: KAG6577643.1 (EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 386.7 bits (992), Expect = 1.3e-103
Identity = 189/223 (84.75%), Postives = 204/223 (91.48%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           MGVRE Q+SD N+VIHELDDLYDSTTG VLTGSWVWNSA VLAEWMAT+ KL DFDFRDK
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
           TVIELGAGAGLPGLMAALLGA  VVLTDVEPLLPGLL+N DANGL DRVEVRELVWGLD+
Sbjct: 61  TVIELGAGAGLPGLMAALLGARHVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
            VS  N+ GEFDLILMSDLFYDSE+MAPLAHILK ISGK+SKIWAASEIRPWTVECLTEF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 MNQGFKIEETPVQLDQPCEE-DDYAILESFSIFHLQPPDEEES 223
           MN+GFK+EE+ VQLD+P EE DD+AIL+SFSIFHLQPPDEE +
Sbjct: 181 MNEGFKVEESQVQLDEPREEVDDHAILDSFSIFHLQPPDEERN 223

[Close]

BLAST of Lag0017391 vs. NCBI nr
Match: XP_022965153.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita maxima])

HSP 1 Score: 382.9 bits (982), Expect = 1.9e-102
Identity = 186/224 (83.04%), Postives = 204/224 (91.07%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           MGVRE Q+SDRN+VIHELDDLYDSTTG VLTGSWVWNSA VLAEWMAT+ KL DFD RDK
Sbjct: 31  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDK 90

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
           TVIELGAG GLPGLMAALLGA RVVLTDVEPLLPGLL+N DANGL DRV+VRELVWGLD+
Sbjct: 91  TVIELGAGVGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLVDRVQVRELVWGLDE 150

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
            VS  N+LGEFDLILMSDLFYDSE+MAPLAH+LK ISGK+SKIWAASEIRPWTVECL EF
Sbjct: 151 SVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTVECLKEF 210

Query: 181 MNQGFKIEETPVQLDQPCEE-DDYAILESFSIFHLQPPDEEESN 224
           MN+GFK+EE+ VQLD+P EE DD+AIL+ FSIFHLQPPDEE ++
Sbjct: 211 MNEGFKVEESQVQLDEPREEDDDHAILDLFSIFHLQPPDEERNS 254

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BLAST of Lag0017391 vs. ExPASy Swiss-Prot
Match: A4FV98 (EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.2e-14
Identity = 55/157 (35.03%), Postives = 84/157 (53.50%), Query Frame = 0


Query: 35  VWNSALVLAEWMATRSKLLDFDFRDKTVIELGAGAGLPGLMAALLGASRVVLTDVEPLLP 94
           VW++AL L  +  ++    + DFR K VIELGAG G+ G++AAL G   V +TD+  +L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLVLE 115

Query: 95  GLLKNADAN-GLGDRVEVRELVWGLDKPVSEVNKLGEFDLILMSDLFYDSEEMAPLAHIL 154
            +  N  AN   G R +VR L WG+D+ V      G++DL+L +D+ Y       L   L
Sbjct: 116 QIQGNVQANVPPGGRAQVRALSWGIDQHVFP----GDYDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KTISGKDSKIWAASEIRP--WTVECLTEFMNQGFKIE 189
           + + G    I+ AS++R    T       + Q F++E
Sbjct: 176 RHLCGPHGTIYLASKMREEHGTESFFQHLLPQHFQLE 203

[Close]

BLAST of Lag0017391 vs. ExPASy Swiss-Prot
Match: Q5BLD8 (Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.2e-14
Identity = 57/158 (36.08%), Postives = 84/158 (53.16%), Query Frame = 0


Query: 35  VWNSALVLAEWMATRSKLLDFDFRDKTVIELGAGAGLPGLMAALLGASRVVLTDVEPLLP 94
           VW++A+VL  ++    ++   D + K VIELGAG GL G++AALLGA+ V +TD EP L 
Sbjct: 46  VWDAAVVLCMFL----EMGKVDLKGKRVIELGAGTGLVGIVAALLGAN-VTITDREPALE 105

Query: 95  GLLKNADAN---GLGDRVEVRELVWGLDKPVSEVNKLGEFDLILMSDLFYDSEEMAPLAH 154
            L  N   N   G    V+V EL WG +    ++   G +DLIL +D+ Y  E    L  
Sbjct: 106 FLTANVHENIPQGRQKAVQVSELTWGEN---LDLYPQGGYDLILGADIVYLEETFPALLQ 165

Query: 155 ILKTISGKDSKIWAASEIRPWTVECLTEFMNQGFKIEE 190
            L+ +S  D+ +  +  IR    E     + Q F ++E
Sbjct: 166 TLEHLSSGDTVVLLSCRIRYERDERFLTELRQRFSVQE 195

[Close]

BLAST of Lag0017391 vs. ExPASy Swiss-Prot
Match: Q8C436 (Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=2 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 1.7e-13
Identity = 63/178 (35.39%), Postives = 89/178 (50.00%), Query Frame = 0


Query: 32  GSWVWNSALVLAEWMATRSKLLD--FDFRDKTVIELGAGAGLPGLMAALLGASRVVLTDV 91
           G  VW++A+VL++++ T     D       ++V+ELG+G G  GLMAA LGA  V++TD+
Sbjct: 39  GCVVWDAAIVLSKYLETPGFSGDGAHALSRRSVLELGSGTGAVGLMAATLGAD-VIVTDL 98

Query: 92  EPLLPGLLKNADANG--LGDRVEVRELVWGLDKPVSEVNKLGEFDLILMSDLFYDSEEMA 151
           E L   L  N D N   +   V+ + L WG D     +  L   D ILM+D  Y  E + 
Sbjct: 99  EELQDLLKMNIDMNKHLVTGSVQAKVLKWGED-----IEDLMSPDYILMADCIYYEESLE 158

Query: 152 PLAHILKTISGKDSKIWAASEIR-----PWTVECLTEFMNQGFKIEETPVQLDQPCEE 201
           PL   LK +SG ++ I    E R     P   +   E +   F  EE P  LD+  EE
Sbjct: 159 PLLKTLKDLSGSETCIICCYEQRTMGKNPEIEKKYFELLQLDFDFEEIP--LDKHDEE 208

[Close]

BLAST of Lag0017391 vs. ExPASy Swiss-Prot
Match: D3YWP0 (EEF1A lysine methyltransferase 3 OS=Mus musculus OX=10090 GN=eef1akmt3 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.1e-13
Identity = 50/137 (36.50%), Postives = 74/137 (54.01%), Query Frame = 0


Query: 35  VWNSALVLAEWMATRSKLLDFDFRDKTVIELGAGAGLPGLMAALLGASRVVLTDVEPLLP 94
           VW++AL L ++  ++    + DFR K VIELGAG G+ G++AAL G   V +TD+   L 
Sbjct: 56  VWDAALSLCDYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPVALE 115

Query: 95  GLLKNADAN-GLGDRVEVRELVWGLDKPVSEVNKLGEFDLILMSDLFYDSEEMAPLAHIL 154
            +  N  AN   G R  V  L WG+D+ V      G +DL+L +D+ Y       L   L
Sbjct: 116 QIQDNVHANVPPGGRARVCALSWGIDQHVFP----GNYDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KTISGKDSKIWAASEIR 171
           + + G    I+ AS++R
Sbjct: 176 RHLCGPHGTIYLASKMR 183

[Close]

BLAST of Lag0017391 vs. ExPASy Swiss-Prot
Match: Q96AZ1 (EEF1A lysine methyltransferase 3 OS=Homo sapiens OX=9606 GN=EEF1AKMT3 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 53/157 (33.76%), Postives = 80/157 (50.96%), Query Frame = 0


Query: 35  VWNSALVLAEWMATRSKLLDFDFRDKTVIELGAGAGLPGLMAALLGASRVVLTDVEPLLP 94
           VW++AL L  +  ++    + DFR K VIELGAG G+ G++AAL G   V +TD+   L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLALE 115

Query: 95  GLLKNADAN-GLGDRVEVRELVWGLDKPVSEVNKLGEFDLILMSDLFYDSEEMAPLAHIL 154
            +  N  AN   G + +VR L WG+D  V   N    +DL+L +D+ Y       L   L
Sbjct: 116 QIQGNVQANVPAGGQAQVRALSWGIDHHVFPAN----YDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KTISGKDSKIWAASEIRP--WTVECLTEFMNQGFKIE 189
           + +      I+ AS++R    T       + Q F++E
Sbjct: 176 QHLCRPHGTIYLASKMRKEHGTESFFQHLLPQHFQLE 203

[Close]

BLAST of Lag0017391 vs. ExPASy TrEMBL
Match: A0A6J1E9L9 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 GN=LOC111431122 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.8e-105
Identity = 190/224 (84.82%), Postives = 206/224 (91.96%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           MGVRE Q+SD N+VIHELDDLYDSTTG VLTGSWVWNSA VLAEWMAT+ KL DFDFRDK
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
           TVIELGAGAGLPGLMAALLGA RVVLTDVEPLLPGLL+N DANGL DRVEVRELVWGLD+
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
            VS  N+ GEFDLILMSDLFYDSE+MAPLAHILK ISGK+SKIWAASEIRPWTVECLTEF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 MNQGFKIEETPVQLDQPCEE-DDYAILESFSIFHLQPPDEEESN 224
           MN+GFK+EE+ VQLD+P EE DD+AIL+SFSIFHLQPPDEE ++
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERNS 224

[Close]

BLAST of Lag0017391 vs. ExPASy TrEMBL
Match: A0A6J1HN15 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=LOC111465095 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 9.3e-103
Identity = 186/224 (83.04%), Postives = 204/224 (91.07%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           MGVRE Q+SDRN+VIHELDDLYDSTTG VLTGSWVWNSA VLAEWMAT+ KL DFD RDK
Sbjct: 31  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDK 90

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
           TVIELGAG GLPGLMAALLGA RVVLTDVEPLLPGLL+N DANGL DRV+VRELVWGLD+
Sbjct: 91  TVIELGAGVGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLVDRVQVRELVWGLDE 150

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
            VS  N+LGEFDLILMSDLFYDSE+MAPLAH+LK ISGK+SKIWAASEIRPWTVECL EF
Sbjct: 151 SVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTVECLKEF 210

Query: 181 MNQGFKIEETPVQLDQPCEE-DDYAILESFSIFHLQPPDEEESN 224
           MN+GFK+EE+ VQLD+P EE DD+AIL+ FSIFHLQPPDEE ++
Sbjct: 211 MNEGFKVEESQVQLDEPREEDDDHAILDLFSIFHLQPPDEERNS 254

[Close]

BLAST of Lag0017391 vs. ExPASy TrEMBL
Match: A0A6J1DMN9 (protein N-lysine methyltransferase METTL21A-like OS=Momordica charantia OX=3673 GN=LOC111022654 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 9.3e-103
Identity = 185/220 (84.09%), Postives = 203/220 (92.27%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           MGVREIQVSD NI+I+ELDDLYDSTTG VLTGSWVWNSA+VLAEWMATR KLLDFDF+DK
Sbjct: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
           TVIELGAGAGLPGLMAALLGASRVVLTD+E LLPGLL+N DANGLGDRVEVRELVWG DK
Sbjct: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
            VS+ N+LGEFDLILMSD+F+D EEM PLA ILK I+G++S IWAASE+RPWT+ECL EF
Sbjct: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180

Query: 181 MNQGFKIEETPVQLDQPCEEDDYAILESFSIFHLQPPDEE 221
            NQGFKI+ETPVQLD+PCEEDDYAIL+SFSIFHLQP D E
Sbjct: 181 TNQGFKIDETPVQLDRPCEEDDYAILDSFSIFHLQPSDGE 220

[Close]

BLAST of Lag0017391 vs. ExPASy TrEMBL
Match: A0A0A0L6N4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.9e-90
Identity = 167/223 (74.89%), Postives = 191/223 (85.65%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           M V+E Q+SDRNIVIHELDDLYDSTTG VLTGSWVWNSA VLA+WMAT+  L DFDFR K
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
            VIELGAG GLPGL AALLGA+RV+LTDVEPLLPGLL+N DANG+GDRVEVRELVWG + 
Sbjct: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
             S+ N+LGEFDLILMSDLFY+SEEM  LA +LK ISG D+KIWAASEIRPWT+EC+TE 
Sbjct: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180

Query: 181 MNQGFKIEETPVQLDQPCEEDDYAILESFSIFHLQPPDEEESN 224
           +NQGFKI E+PVQ D+  EED++ IL+SFSIFHL+   +EE N
Sbjct: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLR---QEERN 220

[Close]

BLAST of Lag0017391 vs. ExPASy TrEMBL
Match: A0A5A7UCU5 (Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G001920 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 2.2e-88
Identity = 168/224 (75.00%), Postives = 191/224 (85.27%), Query Frame = 0


Query: 1   MGVREIQVSDRNIVIHELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFDFRDK 60
           M V+E Q+SDRNIVIHELDDLYDSTTG VLTGSWVWNSA VLAEWMAT+ KL DFD R K
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANGLGDRVEVRELVWGLDK 120
            VIELGAGAGLPGL AALLGA+RVVLTDV+PLL GLL+N DAN LGDRVEVRELVWG ++
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 PVSEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTVECLTEF 180
             S+ N+LGEFDLILMSDLFYDSEEM  LA +LK ISG DSKIWAASEIRPWT+EC+TE 
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 MNQGFKIEETPVQL-DQPCEEDDYAILESFSIFHLQPPDEEESN 224
           +NQGFKI E+PVQ  D+  EED++ +L+S+SIFHL+   +EE N
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLR---QEERN 221

[Close]

BLAST of Lag0017391 vs. TAIR 10
Match: AT5G44170.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 2.5e-15
Identity = 59/199 (29.65%), Postives = 94/199 (47.24%), Query Frame = 0


Query: 27  GTVLTGSWVWNSALVLAEWMATRSKLLD--------------FDFRDKTVIELGAGAGLP 86
           G++  G+ VW  +L+L+++ A R   LD              FDFR +  IELG G G+ 
Sbjct: 25  GSMHVGTSVWPCSLILSKF-AERWSTLDSSSSTTSPNPYAELFDFRRRRGIELGTGCGVA 84

Query: 87  GLMAALLGASRVVLTDVEPLLPGLLKNADAN--GLGDRVEVRELVWGLDKPVSEVNKLGE 146
           G+   LLG + +VLTD+ P++P L  N   N   LG  ++   + W     +S +     
Sbjct: 85  GMAFYLLGLTEIVLTDIAPVMPALKHNLKRNKTALGKSLKTSIVYWNNRDQISALKP--P 144

Query: 147 FDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIR-PWTVECLTEFMNQGFKIEE 206
           FDL++ +D+ Y  E +  L   ++ +   D  +    +IR P   +   E  +  FKIE+
Sbjct: 145 FDLVIAADVVYIEESVGQLVTAMELLVADDGAVLLGYQIRSPEADKLFWELCDIVFKIEK 204

[Close]

BLAST of Lag0017391 vs. TAIR 10
Match: AT1G08125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.8e-14
Identity = 54/190 (28.42%), Postives = 91/190 (47.89%), Query Frame = 0


Query: 16  HELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFD---FRDKTVIELGAGAGLP 75
           HELD   D  +  +  G+ VW++++V A+++   S+   F     + K  IELGAG G+ 
Sbjct: 21  HELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVA 80

Query: 76  GLMAALLGASRVVLTDVEPLLPGLLKNADAN----------GLGDRVEVRELVWGLDKPV 135
           G   A+LG   VV TD + +LP L +N + N               + V EL WG +  +
Sbjct: 81  GFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHI 140

Query: 136 SEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTV-ECLTEFM 192
           + V     FD ++ +D+ Y  + + PL   +  +SG  + +    EIR   V E + +  
Sbjct: 141 TAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMW 200

[Close]

BLAST of Lag0017391 vs. TAIR 10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.8e-14
Identity = 54/190 (28.42%), Postives = 91/190 (47.89%), Query Frame = 0


Query: 16  HELDDLYDSTTGTVLTGSWVWNSALVLAEWMATRSKLLDFD---FRDKTVIELGAGAGLP 75
           HELD   D  +  +  G+ VW++++V A+++   S+   F     + K  IELGAG G+ 
Sbjct: 28  HELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVA 87

Query: 76  GLMAALLGASRVVLTDVEPLLPGLLKNADAN----------GLGDRVEVRELVWGLDKPV 135
           G   A+LG   VV TD + +LP L +N + N               + V EL WG +  +
Sbjct: 88  GFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHI 147

Query: 136 SEVNKLGEFDLILMSDLFYDSEEMAPLAHILKTISGKDSKIWAASEIRPWTV-ECLTEFM 192
           + V     FD ++ +D+ Y  + + PL   +  +SG  + +    EIR   V E + +  
Sbjct: 148 TAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMW 207

[Close]

BLAST of Lag0017391 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 3.4e-12
Identity = 50/147 (34.01%), Postives = 76/147 (51.70%), Query Frame = 0


Query: 30  LTGSWVWNSALVLAEWM--ATRSKLLDFDFRDKTVIELGAGAGLPGLMAALLGASRVVLT 89
           +TGS +W+S +VL +++  +  SK+L  +   K ++ELG+G GL G +AALLG +  VLT
Sbjct: 117 VTGSVMWDSGVVLGKFLEHSVDSKVLSLE--GKKIVELGSGCGLVGCIAALLGGN-AVLT 176

Query: 90  DVEPLLPGLLKNADAN----GLGDRVEVRELVWGLDKPVSEVNKLGEFDLILMSDLFYDS 149
           D+   L  L KN   N           V+ELVWG D     +      D +L SD+ Y  
Sbjct: 177 DLPDRLRLLKKNIQTNLHRGNTRGSAIVQELVWGDDPDPDLIEPFP--DYVLGSDVIYSE 236

Query: 150 EEMAPLAHILKTISGKDSKIWAASEIR 171
           E +  L   L  +    + I+ + E+R
Sbjct: 237 EAVHHLVKTLLQLCSDQTTIFLSGELR 258

[Close]

BLAST of Lag0017391 vs. TAIR 10
Match: AT3G50850.1 (Putative methyltransferase family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.4e-10
Identity = 39/124 (31.45%), Postives = 63/124 (50.81%), Query Frame = 0


Query: 62  VIELGAGAGLPGLMAALLGASRVVLTDVEPLLPGLLKNADANG-----LGDRVEVRELVW 121
           ++ELG+G G+ G+ AA    + V +TD+  ++  L  NADAN       G +V V  L W
Sbjct: 92  IVELGSGTGIVGIAAAATLGANVTVTDLPNVIENLKFNADANAQVVAKFGGKVHVASLRW 151

Query: 122 GLDKPVSEVNKLGE-FDLILMSDLFYDSEEMAPLAHILK--TISGKDSKIWAASEIRPWT 178
           G    + +V  LG+  DLIL SD+ Y      PL   L+   + G   +++  + ++ W 
Sbjct: 152 G---EIDDVESLGQNVDLILASDVVYHVHLYEPLLKTLRFLLLEGSSERVFLMAHLKRWK 211

The following BLAST results are available for this feature:

BLAST of Lag0017391 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015681.1	1.2e-104	85.65	EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022923423.1	1.2e-104	84.82	protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata]	[more]
XP_023553140.1	1.0e-103	84.38	protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo]	[more]
KAG6577643.1	1.3e-103	84.75	EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022965153.1	1.9e-102	83.04	protein N-lysine methyltransferase METTL21A-like [Cucurbita maxima]	[more]

BLAST of Lag0017391 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4FV98	1.2e-14	35.03	EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1	[more]
Q5BLD8	1.2e-14	36.08	Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a P...	[more]
Q8C436	1.7e-13	35.39	Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=...	[more]
D3YWP0	5.1e-13	36.50	EEF1A lysine methyltransferase 3 OS=Mus musculus OX=10090 GN=eef1akmt3 PE=3 SV=1	[more]
Q96AZ1	1.1e-12	33.76	EEF1A lysine methyltransferase 3 OS=Homo sapiens OX=9606 GN=EEF1AKMT3 PE=1 SV=1	[more]

BLAST of Lag0017391 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E9L9	5.8e-105	84.82	protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1HN15	9.3e-103	83.04	protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1DMN9	9.3e-103	84.09	protein N-lysine methyltransferase METTL21A-like OS=Momordica charantia OX=3673 ...	[more]
A0A0A0L6N4	6.9e-90	74.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1	[more]
A0A5A7UCU5	2.2e-88	75.00	Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=...	[more]

BLAST of Lag0017391 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44170.1	2.5e-15	29.65	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.1	2.8e-14	28.42	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.2	2.8e-14	28.42	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G73320.1	3.4e-12	34.01	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT3G50850.1	2.4e-10	31.45	Putative methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019410	Lysine methyltransferase	PFAM	PF10294	Methyltransf_16	coord: 31..171 e-value: 1.8E-27 score: 96.1
IPR019410	Lysine methyltransferase	PANTHER	PTHR14614	HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN	coord: 1..218
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 2..199 e-value: 5.0E-43 score: 148.9
None	No IPR available	PANTHER	PTHR14614:SF123	OSJNBA0070O11.4 PROTEIN	coord: 1..218
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 61..145 e-value: 3.1165E-5 score: 39.7207
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 34..189

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017391.1	Lag0017391.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity