Lag0017371 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017371
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	beta-amyrin 11-oxidase-like
Location	chr5: 2865541 .. 2868625 (-)
RNA-Seq Expression	Lag0017371
Synteny	Lag0017371

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTCATAAATTGGTTGATGCTGATTCCTGTGGGATTTGTGTTTTTTGTTGGGGTTTTGAAGAGATTGAATGATTTGTATTATGCAGTGAAGTTGGGGAAGAAATGGGCTGAGCTTCCTCCTGGTGATCTTGGCTGGCCACTTCTCGGTTCTACTTTATCTTTCTTCAAATATTTCACCATTGGTGCACCTCAAAATTTTATTCGAGCCTTTTCGACCAGGTAATTTCTCCTGCATCTCTATCCATTCACTCTATTTTGATAACGTGGGGTGGGAGATTCGAATCCCCAAAATTTTATTCAACTAGCCTAAAGGTTATATTAATTATGACCGGGATATGTGGTTCAAATCCCCACATCCCCATCCCCATCTCCATCCCCACATGTTGTCGAAAGAAAAAAAAAATTAGAGACCAATTTTTAGAATACAATGATTTAAATAAATACAACGACCTAGAAAGTTTACAAACTTGCTAAACATATAACGAAATAAATACAACTTTAAAAGTTCATACCAAAGTCACAATCTTACCTTTTTTTTTTTTCTCTTTTATTTTTTTTTGGATTAGATATGGGAAAGTTGACATGTACAAGACTCACATGTTTGGGAGAGCCACCATACTTGTCTGCACACCTGAGATCTGTCATCAAGTATTAACCGATGAAACCATATTTGCTGTGAGTTATCCCTCAAACATCAGAACCTTATTTGGTAGAAAGACTCTGTTTGAAGCCTCCAAGGAGGAGCACAGGCAGCTCCGACGACTGATAACGGCGCCGATCAGCGGCCACACGGCGCTGGAGATGTACATAAGTCATATTGAACACAGAGTGATTGGTTGCTTAGAGGAGTGGTCGAGCATGGAGAAGCCATTGGAGCTGTTGACAGAGATAAAGGAGCTTACCTTCAAACTTATTTGGAACATTTTCATGGGTTCAACTCCTATGGACTCTTCCATGAGAGAAATAATGGATTTGTTTTATGATATTAGTCGTGGGTTGTTCTCTTTGCCCATCAACTTCCCTGGCTTCTACTTCCATAAATCACTCAAGGTAAGAACAAAAATCATTTCTAAATATATATTCTAGGAGAAAATACATTTTTGGTCCCTCGGCTTTGAAGAATAAGCGTGTTTGGTCCCAGAGTTTTTAACATAGACTTTTTTAGTCCTTGAGTTGTGAGAAATAGGTTTAAAAGGTCCCTAAATTTTAAAATGGACCTTTTTAGTCCATGGGGGTTTGAGAAATAGGTTTAAAAGGTCCCTCGTATTATTTTGTCAAAATTTTTAAAATAATATATTTTAATATTGAATGGTTTTTTAAATTAAAAAAATTAAAAAAGATTCGGGGACTAAACCTTTTAAACCTATTTTTCAAAACTCAGGGACTAAAAAGATTTATTTTGAAAACTTAGGGACCTTTTTAACCTATTTCTTAAAACTTGGGGACTAGAAATGTCTATTTTGAAAACTTAGGGGACCAAAGACATTTATTCGGGATCAAAAAGGTATTTTTACCTTTCTTATATATAGAATTGAATCATTTTTATTATGGAGGATAATATTTAAATTTAAGATTTTATATTGTTTAGCAACTTTTGATATAGTAAGTATAATTTGATATATTTGAACCATTTTGACTCTTATATTGAATAAATAAATATGAAATATTATTTTGTTTCTATTAAGAGTTTTTTTTTTTAAAAAAAATTGTCCTTTAAATGAAGATTTCTAGTGGGGAATTAAGAATTTAATGGAAATTCTCTTTAGAAAAACTATGTAATTGAAATATAAAAAAAAAAAAAAAGGTAAATGGAAAATATTTTAGAATTTCCCATGAAAGATCCAACCCTGATTTCCGTAAAATTAAACGAGGAATTCTTCGAGGATGGAAAATGAAATGGGAGGCAAAGGTGAGGAAGTCCTTCCCTATCCCGACCCCAGCCTCTGCCTCATGGACATCCCTAATTTAGTAATTATATATTATAAATTTTGTCATATTATTCTTTTTGTTCTCTATATATTTGATATCAATTCTTCGTGTTTGGTTATCATTTAACCACAAGAAAGGTATAAATATAGGCTCGAGACAGATTCCTTGAAATACTTCAATCTATTGTGAATGAGAAGAGATTGGTGAACAAAAACAAAGGTAAATGTTGGGAAGCAAAAGATATGATGGATTTGCTAATAGAAGTGAAAGATGAAGATGGTGAAGGGATGGATGATGATACCATTATAAATATGATTTTTGGTATGTTGTTTGCTGGCCATGAGACTTCAGCTTACACTATAATGTGGGCAGTTTTGCTTCTTACTGATCATCCACATATACTCCAAAAAGCCAAGGTTAGAGTCCTTTCCCAACTTGTTAATTGCAATTCTTTGTATATCAATATAATCATAATGCCTAGCTTTATTTTTCCTACTACATATTTACTTTTTTCTTGAATGCATATGTGATTAAAGGATATTTGGCTTGACGGTTAGAGTGTTGTGTAATCTAGGGTTGCAAAATCGAGTATATATTTTAGGTTTGTGAACTTATTTATCCTAAGTTATGAGTCTATTAACTTCACATTGTTATATAAATTATTTAGGGTTCTATCAACTTATGCGGATCAAAACTCCATTATTTAACCTCAAACCATATATAAGTTAGACAAAACTAAGTTTATAATTGCACAATTCAAAATTGAATGTGATTCTTTATGTATTAAGTTTTAATTTCTTAACCAGGTCAATATTTTCTTAATAAGGAGGTTGGTTGTTCGTATATGCATTTTTAATAAATGCATATAAGAGACGACTTATTACCGTTTGTAAAATGTGGATATGAAATGTGACGAGTTGTAACACTCGAGTTAGGAGGAGCACATGAATGAACCGAGGCCACATCCGAATGAGAGAGATCCTGAGGACATGGAAAGCATGGTCAAGATAAGCTTAAAAGAATTGATAGATACTATCTATACCAACAAGGTGCACTTTCCTTTTCGGTGGCTCAATCATAGGAACTTTGAAGTTAAGCGTGCTTGGCTTGGAGAAGTTCTATGTTGGGTGACCTCTTGGGAATTTTCCTAG

mRNA sequence

ATGGAGTTCATAAATTGGTTGATGCTGATTCCTGTGGGATTTGTGTTTTTTGTTGGGGTTTTGAAGAGATTGAATGATTTGTATTATGCAGTGAAGTTGGGGAAGAAATGGGCTGAGCTTCCTCCTGGTGATCTTGGCTGGCCACTTCTCGGTTCTACTTTATCTTTCTTCAAATATTTCACCATTGGTGCACCTCAAAATTTTATTCGAGCCTTTTCGACCAGATATGGGAAAGTTGACATGTACAAGACTCACATGTTTGGGAGAGCCACCATACTTGTCTGCACACCTGAGATCTGTCATCAAGTATTAACCGATGAAACCATATTTGCTGTGAGTTATCCCTCAAACATCAGAACCTTATTTGGTAGAAAGACTCTGTTTGAAGCCTCCAAGGAGGAGCACAGGCAGCTCCGACGACTGATAACGGCGCCGATCAGCGGCCACACGGCGCTGGAGATGTACATAAGTCATATTGAACACAGAGTGATTGGTTGCTTAGAGGAGTGGTCGAGCATGGAGAAGCCATTGGAGCTGTTGACAGAGATAAAGGAGCTTACCTTCAAACTTATTTGGAACATTTTCATGGGTTCAACTCCTATGGACTCTTCCATGAGAGAAATAATGGATTTGTTTTATGATATTAGTCGTGGGTTGTTCTCTTTGCCCATCAACTTCCCTGGCTTCTACTTCCATAAATCACTCAAGGCTCGAGACAGATTCCTTGAAATACTTCAATCTATTGTGAATGAGAAGAGATTGGTGAACAAAAACAAAGGTAAATGTTGGGAAGCAAAAGATATGATGGATTTGCTAATAGAAGTGAAAGATGAAGATGGTGAAGGGATGGATGATGATACCATTATAAATATGATTTTTGGTATGTTGTTTGCTGGCCATGAGACTTCAGCTTACACTATAATGTGGGCAGTTTTGCTTCTTACTGATCATCCACATATACTCCAAAAAGCCAAGGAGGAGCACATGAATGAACCGAGGCCACATCCGAATGAGAGAGATCCTGAGGACATGGAAAGCATGGTCAAGATAAGCTTAAAAGAATTGATAGATACTATCTATACCAACAAGGTGCACTTTCCTTTTCGGTGGCTCAATCATAGGAACTTTGAAGTTAAGCGTGCTTGGCTTGGAGAAGTTCTATGTTGGGTGACCTCTTGGGAATTTTCCTAG

Coding sequence (CDS)

ATGGAGTTCATAAATTGGTTGATGCTGATTCCTGTGGGATTTGTGTTTTTTGTTGGGGTTTTGAAGAGATTGAATGATTTGTATTATGCAGTGAAGTTGGGGAAGAAATGGGCTGAGCTTCCTCCTGGTGATCTTGGCTGGCCACTTCTCGGTTCTACTTTATCTTTCTTCAAATATTTCACCATTGGTGCACCTCAAAATTTTATTCGAGCCTTTTCGACCAGATATGGGAAAGTTGACATGTACAAGACTCACATGTTTGGGAGAGCCACCATACTTGTCTGCACACCTGAGATCTGTCATCAAGTATTAACCGATGAAACCATATTTGCTGTGAGTTATCCCTCAAACATCAGAACCTTATTTGGTAGAAAGACTCTGTTTGAAGCCTCCAAGGAGGAGCACAGGCAGCTCCGACGACTGATAACGGCGCCGATCAGCGGCCACACGGCGCTGGAGATGTACATAAGTCATATTGAACACAGAGTGATTGGTTGCTTAGAGGAGTGGTCGAGCATGGAGAAGCCATTGGAGCTGTTGACAGAGATAAAGGAGCTTACCTTCAAACTTATTTGGAACATTTTCATGGGTTCAACTCCTATGGACTCTTCCATGAGAGAAATAATGGATTTGTTTTATGATATTAGTCGTGGGTTGTTCTCTTTGCCCATCAACTTCCCTGGCTTCTACTTCCATAAATCACTCAAGGCTCGAGACAGATTCCTTGAAATACTTCAATCTATTGTGAATGAGAAGAGATTGGTGAACAAAAACAAAGGTAAATGTTGGGAAGCAAAAGATATGATGGATTTGCTAATAGAAGTGAAAGATGAAGATGGTGAAGGGATGGATGATGATACCATTATAAATATGATTTTTGGTATGTTGTTTGCTGGCCATGAGACTTCAGCTTACACTATAATGTGGGCAGTTTTGCTTCTTACTGATCATCCACATATACTCCAAAAAGCCAAGGAGGAGCACATGAATGAACCGAGGCCACATCCGAATGAGAGAGATCCTGAGGACATGGAAAGCATGGTCAAGATAAGCTTAAAAGAATTGATAGATACTATCTATACCAACAAGGTGCACTTTCCTTTTCGGTGGCTCAATCATAGGAACTTTGAAGTTAAGCGTGCTTGGCTTGGAGAAGTTCTATGTTGGGTGACCTCTTGGGAATTTTCCTAG

Protein sequence

MEFINWLMLIPVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKYFTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLELLTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHETSAYTIMWAVLLLTDHPHILQKAKEEHMNEPRPHPNERDPEDMESMVKISLKELIDTIYTNKVHFPFRWLNHRNFEVKRAWLGEVLCWVTSWEFS

Homology

[Close]

BLAST of Lag0017371 vs. NCBI nr
Match: XP_022923280.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 512.7 bits (1319), Expect = 2.9e-141
Identity = 248/328 (75.61%), Postives = 281/328 (85.67%), Query Frame = 0


Query: 1   MEFINWLMLI-PVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKY 60
           MEFINW+ L+ PVGFV    VLKRLN LYYAVKLGKKW ELPPGDL WPLLGSTLSF K 
Sbjct: 1   MEFINWVFLVLPVGFVLVYEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIR 120
           FT+G PQN+IR F+TRYGKVDMYKTH+FGRA+ILVCTPEIC QVL+DETIF   YP+N++
Sbjct: 61  FTVGPPQNYIRTFTTRYGKVDMYKTHIFGRASILVCTPEICRQVLSDETIFVPGYPANMQ 120

Query: 121 TLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLEL 180
           TLFGR TL  ASK EHR+LRR+ TAPIS HTALEMYI HIEH VI  LEEWSS+EKPL+L
Sbjct: 121 TLFGRNTLHRASKAEHRKLRRVTTAPISSHTALEMYIDHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARD 240
           LTEIKELTFK+IWNIFMGST +D + +E+  L+ DIS G FSLPINFPGFYFHKSLKAR 
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAG 300
           R  EIL SIVNEKRL+ K+KG+ WEAKDMMDL+IEV+DEDGEGMD++TI ++IFG LFAG
Sbjct: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAYTIMWAVLLLTDHPHILQKAKEE 328
            ETSA+T MWA++ LTDHPHI QKAKEE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHIFQKAKEE 328

[Close]

BLAST of Lag0017371 vs. NCBI nr
Match: XP_023007793.1 (beta-amyrin 11-oxidase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 497.7 bits (1280), Expect = 9.5e-137
Identity = 242/328 (73.78%), Postives = 279/328 (85.06%), Query Frame = 0


Query: 1   MEFINWLMLI-PVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKY 60
           MEFIN + LI PVGFV    VLKRLN LYYAVKLGKKW ELPPGDL WPLLGSTLSF K 
Sbjct: 1   MEFINGVFLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKS 60

Query: 61  FTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIR 120
           FT+G P++FIRAF+TRYGKVDMYKTH+FGRA+ILVC PEIC QVL+DETIF   YP++++
Sbjct: 61  FTVGPPESFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQ 120

Query: 121 TLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLEL 180
           TLFGR TL  ASK EHR+LRR+ TAPIS H ALEMY+ HIEH VI  LEEWSS+EKPL+L
Sbjct: 121 TLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARD 240
           LTEIKELTFK+IWNIFMGST +D + +E+  L+ DIS G FSLPINFPGFYFHKSLKAR 
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAG 300
           R  EIL SIVNEKRL+ K+KG+ WEA+DMMDL+IEV+DEDGEGMD++TI ++IFG LFAG
Sbjct: 241 RLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAYTIMWAVLLLTDHPHILQKAKEE 328
            ETSA+T MWA++ LTDHPH+ QKAKEE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHLFQKAKEE 328

[Close]

BLAST of Lag0017371 vs. NCBI nr
Match: XP_022145397.1 (beta-amyrin 11-oxidase-like, partial [Momordica charantia])

HSP 1 Score: 493.8 bits (1270), Expect = 1.4e-135
Identity = 245/332 (73.80%), Postives = 277/332 (83.43%), Query Frame = 0


Query: 6   WLMLIPVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKYFTIGAP 65
           WL+LI   FVF  GVLKRLN  YYAVKLGK WA+LPPGDL WPL GSTLSF K FTIG+P
Sbjct: 1   WLLLILGVFVFVFGVLKRLNGWYYAVKLGKIWAKLPPGDLDWPLFGSTLSFIKCFTIGSP 60

Query: 66  QNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLFGRK 125
           +NFIR FSTRYGKVDMYKT++FGRATILVCTP+I  QVLTDET F  S+P+ ++TLFGRK
Sbjct: 61  ENFIRTFSTRYGKVDMYKTNIFGRATILVCTPKISRQVLTDETKFVPSFPTTMKTLFGRK 120

Query: 126 TLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLELLTEIKE 185
           +L   SK EHR+LRRL TAP+SGH ALEMYI+HIE  VI  LEEW+SM++P+ELLTEIKE
Sbjct: 121 SLMRVSKAEHRRLRRLTTAPVSGHRALEMYINHIEETVIDGLEEWASMQRPMELLTEIKE 180

Query: 186 LTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRFLEIL 245
           LTFK+IWNIFMGST + S MRE+  LFYDIS G FSLPINFPGF+FHKSLKAR R LEIL
Sbjct: 181 LTFKVIWNIFMGSTSIGSCMREMEALFYDISLGFFSLPINFPGFHFHKSLKARKRLLEIL 240

Query: 246 QSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHETSAY 305
           QSIV++KR V KN GK WEAKDMMDL+IEV+DEDGE +DD+TII++IFG LFAGHETSA 
Sbjct: 241 QSIVSKKRFVKKNNGKNWEAKDMMDLMIEVRDEDGEELDDETIIDLIFGKLFAGHETSAD 300

Query: 306 TIMWAVLLLTDHPHILQKAKEEHMNEPRPHPN 338
           T MWAVL LTDHPHI QKAKEE     R  P+
Sbjct: 301 TAMWAVLFLTDHPHIFQKAKEEQEEIIRRRPS 332

[Close]

BLAST of Lag0017371 vs. NCBI nr
Match: XP_023007801.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])

HSP 1 Score: 493.0 bits (1268), Expect = 2.3e-135
Identity = 239/328 (72.87%), Postives = 278/328 (84.76%), Query Frame = 0


Query: 1   MEFINWLMLI-PVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKY 60
           MEFIN + LI P+GFV    VLKRLN LYYA+KLGKKW ELPPGDL WPLLGSTLSF K 
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIR 120
           FT+G P++FIRAF+TRYGKVDMYKTH+FGRA+I+VC PEIC QVL DET F   YP++++
Sbjct: 61  FTVGPPESFIRAFTTRYGKVDMYKTHIFGRASIIVCKPEICRQVLIDETKFVPGYPASMK 120

Query: 121 TLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLEL 180
           TLFGRK+L   SK EHR+LRR+ TAPISGH ALEMYI HIEH VI  LEEWSS+EKPL+L
Sbjct: 121 TLFGRKSLHRVSKVEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARD 240
           LTEIKELTFK+IWNIFMGST +D + +E+  L+ DIS G FSLPINFPGFYFHKSLKAR 
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAG 300
           R  EIL SIVNEKRL+ K+KG+ WEA+DMMDL+IEV+DEDGEGMD++TI ++IFG LFAG
Sbjct: 241 RLHEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAYTIMWAVLLLTDHPHILQKAKEE 328
            ETSA+T MWA++ LTDHPH+ QKAKEE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHLFQKAKEE 328

[Close]

BLAST of Lag0017371 vs. NCBI nr
Match: XP_022923281.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 492.3 bits (1266), Expect = 4.0e-135
Identity = 240/328 (73.17%), Postives = 277/328 (84.45%), Query Frame = 0


Query: 1   MEFINWLMLI-PVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKY 60
           MEFIN + LI P+GFV    VLKRLN LYYA+KLGKKW ELPPGDL WPLLGSTLSF K 
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWVELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIR 120
           FT+G P++FIR F+TRYGKVDMYKTH+FGRA+ILVC PEIC QVL +ET F   YP++++
Sbjct: 61  FTVGPPESFIRTFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLINETKFVPGYPASMK 120

Query: 121 TLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLEL 180
           TLFGRK+L   SK EHR+LRR+ TAPISGH ALEMYI HIEH VI  LEEWSS+EKPL+L
Sbjct: 121 TLFGRKSLHRVSKAEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARD 240
           LTEIKELTFK+IWNIFMGST +D + +E+  L+ DIS G FSLPINFPGFYFHKSLKAR 
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAG 300
           R  EIL SIVNEKRL+ K+KG+ WEAKDMMDL+IEV+DEDGEGMD++TI ++IFG LFAG
Sbjct: 241 RLHEILLSIVNEKRLMMKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAYTIMWAVLLLTDHPHILQKAKEE 328
            ETSA+T MWA++ LTDHPHI QKAKEE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHIFQKAKEE 328

[Close]

BLAST of Lag0017371 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.6e-84
Identity = 159/366 (43.44%), Postives = 244/366 (66.67%), Query Frame = 0


Query: 4   INWLML----IPVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKY 63
           ++W+ +    + V ++F    ++ LN  YY VKL +K   LPPGD+GWPL+G  LSF K 
Sbjct: 3   VHWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKD 62

Query: 64  FTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIR 123
           F+ G P +FI     +YG+  +YKTH+FG  +I+VC P++C +VLTD+  F + YP +I+
Sbjct: 63  FSSGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIK 122

Query: 124 TLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLEL 183
            L   + + + S  EHR  RRLIT+PI GH AL MY+  +E  VI  LEE SSM+ P+EL
Sbjct: 123 ELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVEL 182

Query: 184 LTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARD 243
           L E+K+++FK I ++FMGS+  D  +++I   F D+  G+FS+PIN PGF FHK+L+AR 
Sbjct: 183 LKEMKKVSFKAIVHVFMGSSNQD-IIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARK 242

Query: 244 RFLEILQSIVNEKRLVNKN-KGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFA 303
           +  +I+Q +V+E+RL+ +N   +  + KD++D+L+EVKDE+G  ++D+ I +++ G+LFA
Sbjct: 243 KLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFA 302

Query: 304 GHETSAYTIMWAVLLLTDHPHILQKAKEEHMNEPRPHPNERDPEDMESMVKISLKELIDT 363
           GHE++A ++MW++  LT HPHIL+KAKEE     R            S  ++SLKE+   
Sbjct: 303 GHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTR--------FSSQKQLSLKEIKQM 359

Query: 364 IYTNKV 365
           +Y ++V
Sbjct: 363 VYLSQV 359

[Close]

BLAST of Lag0017371 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 277.3 bits (708), Expect = 2.6e-73
Identity = 156/362 (43.09%), Postives = 222/362 (61.33%), Query Frame = 0


Query: 6   WLMLIPVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKYFTIGAP 65
           W  LI +G      VLKR+N   Y  KLG+K   LPPGDLGWP++G+  SF + F    P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 66  QNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLFGRK 125
           ++FI+++ TRYG+  +YK HMFG   +LV TPE C +VLTD+  F + +P +   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 126 TLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLELLTEIKE 185
           +    S EEH++LRRL +AP++G  AL +YI  IE  V   LE+WS M + +E L+ +++
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 189

Query: 186 LTFKLIWNIFMGSTP---MDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRFL 245
           LTFK+I  IF+ S     MDS  RE  +L Y    G+ ++ IN PGF +H++LKAR + +
Sbjct: 190 LTFKVIMYIFLSSESEHVMDSLEREYTNLNY----GVRAMGINLPGFAYHRALKARKKLV 249

Query: 246 EILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHET 305
              QSIV  +R   K        KDM+D LI+VKDE+G  +DD+ II+++   L AGHE+
Sbjct: 250 AAFQSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 309

Query: 306 SAYTIMWAVLLLTDHPHILQKAKEEHMNEPRPHPNERDPEDMESMVKISLKELIDTIYTN 365
           S +  MWA +L+ +HP ILQKAKEE          ER  +      K++LKE  + +Y +
Sbjct: 310 SGHLTMWATILMQEHPMILQKAKEE---------QERIVKKRAPGQKLTLKETREMVYLS 356

[Close]

BLAST of Lag0017371 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.8e-67
Identity = 137/325 (42.15%), Postives = 199/325 (61.23%), Query Frame = 0


Query: 6   WLMLIPVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKYFTIGAP 65
           W  L+ +G      +++++N   Y   LG+    LPPGDLGWP +G+ LSF + F    P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 66  QNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLFGRK 125
            +F R    RYG   +YK HMFG  +I+V T + C +VLTD+  F   +P++   L GRK
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 126 TLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLELLTEIKE 185
           +    S EEH++LRRL  AP++GH AL  YI +IE  VI  L++W+ M +  E LT +++
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRK 190

Query: 186 LTFKLIWNIFMGS---TPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRFL 245
           LTF++I  IF+ S     MD+  RE   L Y    G+ ++ +N PGF +H++LKAR   +
Sbjct: 191 LTFRIIMYIFLSSESENVMDALEREYTALNY----GVRAMAVNIPGFAYHRALKARKTLV 250

Query: 246 EILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHET 305
              QSIV E+R   K +      KDM+D L+ VKDEDG+ +DD+ II+++   L AGHE+
Sbjct: 251 AAFQSIVTERRNQRK-QNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHES 310

Query: 306 SAYTIMWAVLLLTDHPHILQKAKEE 328
           S +TIMWA + L +HP +LQ+AK E
Sbjct: 311 SGHTIMWATVFLQEHPEVLQRAKAE 329

[Close]

BLAST of Lag0017371 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 4.4e-60
Identity = 129/357 (36.13%), Postives = 205/357 (57.42%), Query Frame = 0


Query: 6   WLMLIPV--GFVFFVGVLKRLNDLYYAVKLG-KKWAELPPGDLGWPLLGSTLSFFKYFTI 65
           W++   V  G V    V++R +D      LG ++ + LPPG++GWP++GS  +F + F  
Sbjct: 18  WVLAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKS 77

Query: 66  GAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLF 125
           G P  FI +F  R+G+  +Y+T MF   TIL  TPE C QVL D+  F   +P    TL 
Sbjct: 78  GNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLI 137

Query: 126 GRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEK-PLELLT 185
           G K+    S ++HR++R+L  API+G  AL  Y+S I+  V+  L  WSS E   +E LT
Sbjct: 138 GPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLT 197

Query: 186 EIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRF 245
           E++ +TFK+I  IFM     D++M  +   + D++ G+ ++ IN PGF ++++L+AR + 
Sbjct: 198 ELRRMTFKIIVQIFMSGAD-DATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKL 257

Query: 246 LEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHE 305
           + +LQ +++ +R       K   A DMMD LIE +DE G  + DD I++++   L AGHE
Sbjct: 258 VSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHE 317

Query: 306 TSAYTIMWAVLLLTDHPHILQKAKEEHMNEPRPHPNERDPEDMESMVKIS-LKELID 358
           +S +  MWA + L ++P I  +AK E     R  P  ++   +    K+  L +++D
Sbjct: 318 SSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVD 373

[Close]

BLAST of Lag0017371 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 7.7e-57
Identity = 116/304 (38.16%), Postives = 176/304 (57.89%), Query Frame = 0


Query: 28  YYAVKLGK-KWAELPPGDLGWPLLGSTLSFFKYFTIGAPQNFIRAFSTRYGKVDMYKTHM 87
           Y+   LG  +   LPPGD+GWPL+G   +F + F  G P +FI +F+ R+G+  +Y+  M
Sbjct: 32  YWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPDSFIDSFARRFGRAGLYRAFM 91

Query: 88  FGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLFGRKTLFEASKEEHRQLRRLITAPI 147
           F   TI+  TPE C QVL D+  F   +P     L G K+      +EHR+LR+L  API
Sbjct: 92  FSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRLRKLTAAPI 151

Query: 148 SGHTALEMYISHIEHRVIGCLEEWSSM--EKPLELLTEIKELTFKLIWNIFMGSTPMDSS 207
           +G  AL  Y+  I+  V+  L  WS    +   E LTE++ +TF++I  IFMG    + +
Sbjct: 152 NGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFMGGAD-ERT 211

Query: 208 MREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRFLEILQSIVNEKRL-VNKNKGKCW 267
             E+   + +++ G+ ++ I+ PGF +HK+++AR R +  LQ +++E+R    K      
Sbjct: 212 AAELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERRARGGKTAAGAA 271

Query: 268 EAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHETSAYTIMWAVLLLTDHPHILQK 327
              DMMD LI V+DE G  + DD II+++   L AGHE+S +  MWA + L ++P IL K
Sbjct: 272 APVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPEILAK 331

[Close]

BLAST of Lag0017371 vs. ExPASy TrEMBL
Match: A0A6J1EBC2 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431021 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.4e-141
Identity = 248/328 (75.61%), Postives = 281/328 (85.67%), Query Frame = 0


Query: 1   MEFINWLMLI-PVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKY 60
           MEFINW+ L+ PVGFV    VLKRLN LYYAVKLGKKW ELPPGDL WPLLGSTLSF K 
Sbjct: 1   MEFINWVFLVLPVGFVLVYEVLKRLNGLYYAVKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIR 120
           FT+G PQN+IR F+TRYGKVDMYKTH+FGRA+ILVCTPEIC QVL+DETIF   YP+N++
Sbjct: 61  FTVGPPQNYIRTFTTRYGKVDMYKTHIFGRASILVCTPEICRQVLSDETIFVPGYPANMQ 120

Query: 121 TLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLEL 180
           TLFGR TL  ASK EHR+LRR+ TAPIS HTALEMYI HIEH VI  LEEWSS+EKPL+L
Sbjct: 121 TLFGRNTLHRASKAEHRKLRRVTTAPISSHTALEMYIDHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARD 240
           LTEIKELTFK+IWNIFMGST +D + +E+  L+ DIS G FSLPINFPGFYFHKSLKAR 
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAG 300
           R  EIL SIVNEKRL+ K+KG+ WEAKDMMDL+IEV+DEDGEGMD++TI ++IFG LFAG
Sbjct: 241 RLYEILLSIVNEKRLMKKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAYTIMWAVLLLTDHPHILQKAKEE 328
            ETSA+T MWA++ LTDHPHI QKAKEE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHIFQKAKEE 328

[Close]

BLAST of Lag0017371 vs. ExPASy TrEMBL
Match: A0A6J1L8M7 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500295 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 4.6e-137
Identity = 242/328 (73.78%), Postives = 279/328 (85.06%), Query Frame = 0


Query: 1   MEFINWLMLI-PVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKY 60
           MEFIN + LI PVGFV    VLKRLN LYYAVKLGKKW ELPPGDL WPLLGSTLSF K 
Sbjct: 1   MEFINGVFLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKS 60

Query: 61  FTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIR 120
           FT+G P++FIRAF+TRYGKVDMYKTH+FGRA+ILVC PEIC QVL+DETIF   YP++++
Sbjct: 61  FTVGPPESFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQ 120

Query: 121 TLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLEL 180
           TLFGR TL  ASK EHR+LRR+ TAPIS H ALEMY+ HIEH VI  LEEWSS+EKPL+L
Sbjct: 121 TLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARD 240
           LTEIKELTFK+IWNIFMGST +D + +E+  L+ DIS G FSLPINFPGFYFHKSLKAR 
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAG 300
           R  EIL SIVNEKRL+ K+KG+ WEA+DMMDL+IEV+DEDGEGMD++TI ++IFG LFAG
Sbjct: 241 RLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAYTIMWAVLLLTDHPHILQKAKEE 328
            ETSA+T MWA++ LTDHPH+ QKAKEE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHLFQKAKEE 328

[Close]

BLAST of Lag0017371 vs. ExPASy TrEMBL
Match: A0A6J1CWG2 (beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111014855 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 6.6e-136
Identity = 245/332 (73.80%), Postives = 277/332 (83.43%), Query Frame = 0


Query: 6   WLMLIPVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKYFTIGAP 65
           WL+LI   FVF  GVLKRLN  YYAVKLGK WA+LPPGDL WPL GSTLSF K FTIG+P
Sbjct: 1   WLLLILGVFVFVFGVLKRLNGWYYAVKLGKIWAKLPPGDLDWPLFGSTLSFIKCFTIGSP 60

Query: 66  QNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLFGRK 125
           +NFIR FSTRYGKVDMYKT++FGRATILVCTP+I  QVLTDET F  S+P+ ++TLFGRK
Sbjct: 61  ENFIRTFSTRYGKVDMYKTNIFGRATILVCTPKISRQVLTDETKFVPSFPTTMKTLFGRK 120

Query: 126 TLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLELLTEIKE 185
           +L   SK EHR+LRRL TAP+SGH ALEMYI+HIE  VI  LEEW+SM++P+ELLTEIKE
Sbjct: 121 SLMRVSKAEHRRLRRLTTAPVSGHRALEMYINHIEETVIDGLEEWASMQRPMELLTEIKE 180

Query: 186 LTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRFLEIL 245
           LTFK+IWNIFMGST + S MRE+  LFYDIS G FSLPINFPGF+FHKSLKAR R LEIL
Sbjct: 181 LTFKVIWNIFMGSTSIGSCMREMEALFYDISLGFFSLPINFPGFHFHKSLKARKRLLEIL 240

Query: 246 QSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHETSAY 305
           QSIV++KR V KN GK WEAKDMMDL+IEV+DEDGE +DD+TII++IFG LFAGHETSA 
Sbjct: 241 QSIVSKKRFVKKNNGKNWEAKDMMDLMIEVRDEDGEELDDETIIDLIFGKLFAGHETSAD 300

Query: 306 TIMWAVLLLTDHPHILQKAKEEHMNEPRPHPN 338
           T MWAVL LTDHPHI QKAKEE     R  P+
Sbjct: 301 TAMWAVLFLTDHPHIFQKAKEEQEEIIRRRPS 332

[Close]

BLAST of Lag0017371 vs. ExPASy TrEMBL
Match: A0A6J1L1P3 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.1e-135
Identity = 239/328 (72.87%), Postives = 278/328 (84.76%), Query Frame = 0


Query: 1   MEFINWLMLI-PVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKY 60
           MEFIN + LI P+GFV    VLKRLN LYYA+KLGKKW ELPPGDL WPLLGSTLSF K 
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIR 120
           FT+G P++FIRAF+TRYGKVDMYKTH+FGRA+I+VC PEIC QVL DET F   YP++++
Sbjct: 61  FTVGPPESFIRAFTTRYGKVDMYKTHIFGRASIIVCKPEICRQVLIDETKFVPGYPASMK 120

Query: 121 TLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLEL 180
           TLFGRK+L   SK EHR+LRR+ TAPISGH ALEMYI HIEH VI  LEEWSS+EKPL+L
Sbjct: 121 TLFGRKSLHRVSKVEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARD 240
           LTEIKELTFK+IWNIFMGST +D + +E+  L+ DIS G FSLPINFPGFYFHKSLKAR 
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAG 300
           R  EIL SIVNEKRL+ K+KG+ WEA+DMMDL+IEV+DEDGEGMD++TI ++IFG LFAG
Sbjct: 241 RLHEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAYTIMWAVLLLTDHPHILQKAKEE 328
            ETSA+T MWA++ LTDHPH+ QKAKEE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHLFQKAKEE 328

[Close]

BLAST of Lag0017371 vs. ExPASy TrEMBL
Match: A0A6J1E6E2 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.9e-135
Identity = 240/328 (73.17%), Postives = 277/328 (84.45%), Query Frame = 0


Query: 1   MEFINWLMLI-PVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKY 60
           MEFIN + LI P+GFV    VLKRLN LYYA+KLGKKW ELPPGDL WPLLGSTLSF K 
Sbjct: 1   MEFINGVFLILPLGFVLVFEVLKRLNGLYYALKLGKKWVELPPGDLSWPLLGSTLSFLKS 60

Query: 61  FTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIR 120
           FT+G P++FIR F+TRYGKVDMYKTH+FGRA+ILVC PEIC QVL +ET F   YP++++
Sbjct: 61  FTVGPPESFIRTFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLINETKFVPGYPASMK 120

Query: 121 TLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLEL 180
           TLFGRK+L   SK EHR+LRR+ TAPISGH ALEMYI HIEH VI  LEEWSS+EKPL+L
Sbjct: 121 TLFGRKSLHRVSKAEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKL 180

Query: 181 LTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARD 240
           LTEIKELTFK+IWNIFMGST +D + +E+  L+ DIS G FSLPINFPGFYFHKSLKAR 
Sbjct: 181 LTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARK 240

Query: 241 RFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAG 300
           R  EIL SIVNEKRL+ K+KG+ WEAKDMMDL+IEV+DEDGEGMD++TI ++IFG LFAG
Sbjct: 241 RLHEILLSIVNEKRLMMKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAG 300

Query: 301 HETSAYTIMWAVLLLTDHPHILQKAKEE 328
            ETSA+T MWA++ LTDHPHI QKAKEE
Sbjct: 301 QETSAFTTMWAIIFLTDHPHIFQKAKEE 328

[Close]

BLAST of Lag0017371 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 277.3 bits (708), Expect = 1.9e-74
Identity = 156/362 (43.09%), Postives = 222/362 (61.33%), Query Frame = 0


Query: 6   WLMLIPVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKYFTIGAP 65
           W  LI +G      VLKR+N   Y  KLG+K   LPPGDLGWP++G+  SF + F    P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 66  QNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLFGRK 125
           ++FI+++ TRYG+  +YK HMFG   +LV TPE C +VLTD+  F + +P +   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 126 TLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLELLTEIKE 185
           +    S EEH++LRRL +AP++G  AL +YI  IE  V   LE+WS M + +E L+ +++
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 189

Query: 186 LTFKLIWNIFMGSTP---MDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRFL 245
           LTFK+I  IF+ S     MDS  RE  +L Y    G+ ++ IN PGF +H++LKAR + +
Sbjct: 190 LTFKVIMYIFLSSESEHVMDSLEREYTNLNY----GVRAMGINLPGFAYHRALKARKKLV 249

Query: 246 EILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHET 305
              QSIV  +R   K        KDM+D LI+VKDE+G  +DD+ II+++   L AGHE+
Sbjct: 250 AAFQSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 309

Query: 306 SAYTIMWAVLLLTDHPHILQKAKEEHMNEPRPHPNERDPEDMESMVKISLKELIDTIYTN 365
           S +  MWA +L+ +HP ILQKAKEE          ER  +      K++LKE  + +Y +
Sbjct: 310 SGHLTMWATILMQEHPMILQKAKEE---------QERIVKKRAPGQKLTLKETREMVYLS 356

[Close]

BLAST of Lag0017371 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 277.3 bits (708), Expect = 1.9e-74
Identity = 156/362 (43.09%), Postives = 222/362 (61.33%), Query Frame = 0


Query: 6   WLMLIPVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKYFTIGAP 65
           W  LI +G      VLKR+N   Y  KLG+K   LPPGDLGWP++G+  SF + F    P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 66  QNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLFGRK 125
           ++FI+++ TRYG+  +YK HMFG   +LV TPE C +VLTD+  F + +P +   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 126 TLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLELLTEIKE 185
           +    S EEH++LRRL +AP++G  AL +YI  IE  V   LE+WS M + +E L+ +++
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 189

Query: 186 LTFKLIWNIFMGSTP---MDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRFL 245
           LTFK+I  IF+ S     MDS  RE  +L Y    G+ ++ IN PGF +H++LKAR + +
Sbjct: 190 LTFKVIMYIFLSSESEHVMDSLEREYTNLNY----GVRAMGINLPGFAYHRALKARKKLV 249

Query: 246 EILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHET 305
              QSIV  +R   K        KDM+D LI+VKDE+G  +DD+ II+++   L AGHE+
Sbjct: 250 AAFQSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 309

Query: 306 SAYTIMWAVLLLTDHPHILQKAKEEHMNEPRPHPNERDPEDMESMVKISLKELIDTIYTN 365
           S +  MWA +L+ +HP ILQKAKEE          ER  +      K++LKE  + +Y +
Sbjct: 310 SGHLTMWATILMQEHPMILQKAKEE---------QERIVKKRAPGQKLTLKETREMVYLS 356

[Close]

BLAST of Lag0017371 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 257.3 bits (656), Expect = 2.0e-68
Identity = 137/325 (42.15%), Postives = 199/325 (61.23%), Query Frame = 0


Query: 6   WLMLIPVGFVFFVGVLKRLNDLYYAVKLGKKWAELPPGDLGWPLLGSTLSFFKYFTIGAP 65
           W  L+ +G      +++++N   Y   LG+    LPPGDLGWP +G+ LSF + F    P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 66  QNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEICHQVLTDETIFAVSYPSNIRTLFGRK 125
            +F R    RYG   +YK HMFG  +I+V T + C +VLTD+  F   +P++   L GRK
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 126 TLFEASKEEHRQLRRLITAPISGHTALEMYISHIEHRVIGCLEEWSSMEKPLELLTEIKE 185
           +    S EEH++LRRL  AP++GH AL  YI +IE  VI  L++W+ M +  E LT +++
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRK 190

Query: 186 LTFKLIWNIFMGS---TPMDSSMREIMDLFYDISRGLFSLPINFPGFYFHKSLKARDRFL 245
           LTF++I  IF+ S     MD+  RE   L Y    G+ ++ +N PGF +H++LKAR   +
Sbjct: 191 LTFRIIMYIFLSSESENVMDALEREYTALNY----GVRAMAVNIPGFAYHRALKARKTLV 250

Query: 246 EILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDEDGEGMDDDTIINMIFGMLFAGHET 305
              QSIV E+R   K +      KDM+D L+ VKDEDG+ +DD+ II+++   L AGHE+
Sbjct: 251 AAFQSIVTERRNQRK-QNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHES 310

Query: 306 SAYTIMWAVLLLTDHPHILQKAKEE 328
           S +TIMWA + L +HP +LQ+AK E
Sbjct: 311 SGHTIMWATVFLQEHPEVLQRAKAE 329

[Close]

BLAST of Lag0017371 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 143.3 bits (360), Expect = 4.2e-34
Identity = 86/292 (29.45%), Postives = 148/292 (50.68%), Query Frame = 0


Query: 40  LPPGDLGWPLLGSTLSFFKYFTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEI 99
           LPPG +G P +G TL  +       P +F      +YG  D++KTH+ G   +++ +PE 
Sbjct: 49  LPPGSMGLPYIGETLRLY----TENPNSFFATRQNKYG--DIFKTHILGCPCVMISSPEA 108

Query: 100 CHQVLTDET-IFAVSYPSNIRTLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISH 159
              VL  +  +F  +YP +   + G + LF      H  L+RL+ +     +AL   +SH
Sbjct: 109 ARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFM-PSALRPTVSH 168

Query: 160 IEHRVIGCLEEWSSMEKPLELLTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRG 219
           IE  V+  L  W+S +K +  L  +K   F +      G     +++  I  L+  + RG
Sbjct: 169 IELLVLQTLSSWTS-QKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERG 228

Query: 220 LFSLPINFPGFYFHKSLKARDRFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDE 279
             S+P++ PG  FHKS+KAR    E L+ ++ ++R   + +G       ++ +L+  KD+
Sbjct: 229 YNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAKDQ 288

Query: 280 DGEGMDDDTIINMIFGMLFAGHETSAYTIMWAVLLLTDHPHILQKAKEEHMN 331
              G+ D  I + I G++FA  +T+A  + W +  L DHP++LQ+   E  +
Sbjct: 289 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFS 326

[Close]

BLAST of Lag0017371 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 143.3 bits (360), Expect = 4.2e-34
Identity = 86/292 (29.45%), Postives = 148/292 (50.68%), Query Frame = 0


Query: 40  LPPGDLGWPLLGSTLSFFKYFTIGAPQNFIRAFSTRYGKVDMYKTHMFGRATILVCTPEI 99
           LPPG +G P +G TL  +       P +F      +YG  D++KTH+ G   +++ +PE 
Sbjct: 49  LPPGSMGLPYIGETLRLY----TENPNSFFATRQNKYG--DIFKTHILGCPCVMISSPEA 108

Query: 100 CHQVLTDET-IFAVSYPSNIRTLFGRKTLFEASKEEHRQLRRLITAPISGHTALEMYISH 159
              VL  +  +F  +YP +   + G + LF      H  L+RL+ +     +AL   +SH
Sbjct: 109 ARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFM-PSALRPTVSH 168

Query: 160 IEHRVIGCLEEWSSMEKPLELLTEIKELTFKLIWNIFMGSTPMDSSMREIMDLFYDISRG 219
           IE  V+  L  W+S +K +  L  +K   F +      G     +++  I  L+  + RG
Sbjct: 169 IELLVLQTLSSWTS-QKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERG 228

Query: 220 LFSLPINFPGFYFHKSLKARDRFLEILQSIVNEKRLVNKNKGKCWEAKDMMDLLIEVKDE 279
             S+P++ PG  FHKS+KAR    E L+ ++ ++R   + +G       ++ +L+  KD+
Sbjct: 229 YNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAKDQ 288

Query: 280 DGEGMDDDTIINMIFGMLFAGHETSAYTIMWAVLLLTDHPHILQKAKEEHMN 331
              G+ D  I + I G++FA  +T+A  + W +  L DHP++LQ+   E  +
Sbjct: 289 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFS 326

The following BLAST results are available for this feature:

BLAST of Lag0017371 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923280.1	2.9e-141	75.61	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]
XP_023007793.1	9.5e-137	73.78	beta-amyrin 11-oxidase-like isoform X2 [Cucurbita maxima]	[more]
XP_022145397.1	1.4e-135	73.80	beta-amyrin 11-oxidase-like, partial [Momordica charantia]	[more]
XP_023007801.1	2.3e-135	72.87	beta-amyrin 11-oxidase-like [Cucurbita maxima]	[more]
XP_022923281.1	4.0e-135	73.17	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]

BLAST of Lag0017371 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	2.6e-84	43.44	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	2.6e-73	43.09	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
O23051	2.8e-67	42.15	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q5VRM7	4.4e-60	36.13	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q9AXH9	7.7e-57	38.16	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]

BLAST of Lag0017371 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EBC2	1.4e-141	75.61	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431021 PE=3 S...	[more]
A0A6J1L8M7	4.6e-137	73.78	beta-amyrin 11-oxidase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1115002...	[more]
A0A6J1CWG2	6.6e-136	73.80	beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111014855 PE=3 ...	[more]
A0A6J1L1P3	1.1e-135	72.87	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=...	[more]
A0A6J1E6E2	1.9e-135	73.17	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 S...	[more]

BLAST of Lag0017371 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	1.9e-74	43.09	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	1.9e-74	43.09	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	2.0e-68	42.15	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G29090.1	4.2e-34	29.45	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	4.2e-34	29.45	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 41..329 e-value: 2.1E-30 score: 105.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 32..352 e-value: 5.1E-66 score: 225.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 38..347
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 325..344
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 19..328
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 19..328

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017371.1	Lag0017371.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen