Lag0016535 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016535
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Locationchr12: 38724646 .. 38727568 (-)
RNA-Seq ExpressionLag0016535
SyntenyLag0016535
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAGGGGAAGATCTGATGGAACACAGAAAAAGCGCTTAATCACTACCATATGTGTTGTGGCAATATTTCTTGGGTTTCTATATGCATATTATGGATCCATTTTTGGCAATCAAGACCGTGGTGCATCGGCTCTGCAGCATGGCAGCAAATCCTTGAGCCATTATTTGATGGGGAGTGAAGACTCTGAAGAATCTTCAACCTCAACTGAGCAGGAAGATCCAGAAGATGATGTCGTACCAAAAAGTTACCCGGTACGTAACTGTCTTGCTTTAATAGGTGCTCATTAAAACAACTCTCTTTTCTTGATAAAAAGAGAATAAAACATCCATTGGAACCCATTTCCTATATATTTTTAATTTGTTTCTTCAACTTTCCATATTTTTAATCTTGAGATGAGATCTGTTTAGCCCTCATGTAGTTTTTATTTTTATTTTAAAGGTTTGCGATGACCGGCATTCGGAATTAATTCCCTGCTTGGACAGACATCTCATATATCAAATGAGATTGAAACTGGATCTTTCTTTAATGGAACACTATGAAAGACACTGCCCACCACCTGAGAGGCGGTTCAATTGCCTGATTCCTCCTCCAGCAGGATATAAGGTAGCAATTTGGTAACACTGAGCATCGCCTGAATGTTTTGCTGATTACTAACTACATCATTTCAGCTTATGATTTCTAACTTTTTACTTGTTAACAATGTGTTGCTGCAGGTCCCAATTAAGTGGCCAAAGAGTAGAGATGAAGTGTGGAAAGCTAACATACCTCACACTCATCTTGCACATGAGAAATCTGATCAGAACTGGATGGTTGTCAAAGGTGAAAAAATTGTGTTCCCTGGAGGAGGTACTCATTTTCACTATGGAGCTGATAAGTACATTGCTTCAATTGCCAATGTAAGATCTCTGAAACTTCTTTACCTTCCAGAAAGGAAGGATGACACAACAAAGTTTGCTTTTTGGACAATATTTTCAAATAAAAAGTTCATTAAATGACTCAAATTTGCATGTGTTTAGAGAATATCATGTTTGATCTCTACCATTCATGGTTGTCTCAAGTTTATGAGATTCTGTATTTCTATTCAGAATTCCTAATGAGCAATTGATTAACTGAAAATGGTTGGCCTTCATCTTTCAGATGCTCAACTTTTCAAATGATAATCTAAACAATCAAGGACGATTGCGGACTGTTCTTGATGTTGGTTGTGGTGTTGCAAGTTTTGGAGGATATCTTCTCTCTTCTAATATTATAGCAATGTCCTTAGCACCAAATGATGTTCATCAGAATCAAATTCAGTTTGCTTTGGAAAGAGGAATTCCTGCATACCTTGGTGTTTTAGGAACCAAGAGGCTCCCTTACCCAAGCAGATCTTTTGAACTTGCACACTGTTCTCGTTGTCGTATAGATTGGCTTCAAAGAGACGGGATTCTTCTTCTTGAGTTAGATAGATTACTTAGACCTGGAGGTTATTTTGCTTATTCATCTCCAGAAGCATATGCACAGGATGAGGAGGATCTGAGAATATGGAGAGAAATGAGTGACCTTGTGAGTCGTATGTGTTGGAGAATTGCTGCAAAGAGGAACCAGACTGTGATTTGGCAAAAACCCGTAACAAATGACTGTTATCTGCAAAGAGAACCTGGTACTCGTCCTCCACTTTGCCGATCTGACGATGATCCTGATGCTGTTTGGGGTGTGCAGATGGAAGCTTGTATCACTCCCTACTCTGACCGTAAGTCCCTTATTGAATGGCTTATTTAGATTTTTGTTTTCTTTTAACTAAATGCATCATTTTGTATTTGCTAACTTCAATCCATGTGCTGCTGCTTAGCTAATGGAAATTGTTTGAATTGTTGGGTTTAAAGCAATGTGGCATAGAGCATCTGACATCTTTGGGTTAATGAATACTACCTTTGGACTTCATGGTGTTGATTTTTTCTTTGTTATGACCCATGTAGTTTTGAGATCTTTTCCTTGTGTGCTATTTGACTCGTGCCGAGATATATCACCCTATTGCAAATTGAATATGCTTGCTAAGTGATCACATTGGATTTGTTGATGCAGATGATCATAAAGCCAAGGGCAGTGGATTAGCTCCTTGGCCAGCTCGATTAACTTCACCTCCTCCACGTCTTCAGGATTTTGGCTATTCAACTGAAATGTTTGAAAAAGACACGGTAATTTATGAGCAGGGTGAATTTGGCTAATTCTAATCTGGATGCCAAAAGTCTCTAAATTTTATCATCTTGTTGCAGGAAATTTGGCGGCGTAGAGTCGAGAGTTACTGGAATCTACTGAGTCCAAAGATCGAATCAGATACTATTAGAAACGTGATGGACATGAAGGCAAATATGGGATCATTTGGAGCTGCTCTGAAGGACAAGGATGTGTGGGTGATGAACGTTGTCCCAGAAGACGGACCGAACACACTGAAGTTGATATACGACCGAGGCCTCATAGGCACTACTAATAACTGGTATGCAACCTGAAAAAGCACTTGCATATCCTTATGGCTTCAATGAACTGTGAAATGCAATTCTAGTTTATATTCAAGATAGGATGTTCCAGTTGATGACACTTCATTACATTTTCAACAGGTGTGAAGCCTTCTCGACATATCCTCGTACCTACGATCTGCTCCACGCATGGACCGTCTTTTCCGACGTCGAGAAGAAAGAATGTGGCGCTGAGGACCTGTTGCTCGAGATGGATCGCATGCTCAGACCCACAGGTTTCATCATCATCAGGGACAAGCAATCCGTTGTAGATTTGATAAAGAAGTACCTACCTGCATTGCACTGGGAAACAGTGGCAACCACGGATGCAAGCTCGGATTCAGAATTGGACAGCGACGAGGTCGTGTTTATCGTCCAGAAGAAACTGTGGCTGACAAGTGAGAGTGTGAGGGATTCAGAATAA

mRNA sequence

ATGACGAGGGGAAGATCTGATGGAACACAGAAAAAGCGCTTAATCACTACCATATGTGTTGTGGCAATATTTCTTGGGTTTCTATATGCATATTATGGATCCATTTTTGGCAATCAAGACCGTGGTGCATCGGCTCTGCAGCATGGCAGCAAATCCTTGAGCCATTATTTGATGGGGAGTGAAGACTCTGAAGAATCTTCAACCTCAACTGAGCAGGAAGATCCAGAAGATGATGTCGTACCAAAAAGTTACCCGGTTTGCGATGACCGGCATTCGGAATTAATTCCCTGCTTGGACAGACATCTCATATATCAAATGAGATTGAAACTGGATCTTTCTTTAATGGAACACTATGAAAGACACTGCCCACCACCTGAGAGGCGGTTCAATTGCCTGATTCCTCCTCCAGCAGGATATAAGGTCCCAATTAAGTGGCCAAAGAGTAGAGATGAAGTGTGGAAAGCTAACATACCTCACACTCATCTTGCACATGAGAAATCTGATCAGAACTGGATGGTTGTCAAAGGTGAAAAAATTGTGTTCCCTGGAGGAGGTACTCATTTTCACTATGGAGCTGATAAGTACATTGCTTCAATTGCCAATATGCTCAACTTTTCAAATGATAATCTAAACAATCAAGGACGATTGCGGACTGTTCTTGATGTTGGTTGTGGTGTTGCAAGTTTTGGAGGATATCTTCTCTCTTCTAATATTATAGCAATGTCCTTAGCACCAAATGATGTTCATCAGAATCAAATTCAGTTTGCTTTGGAAAGAGGAATTCCTGCATACCTTGGTGTTTTAGGAACCAAGAGGCTCCCTTACCCAAGCAGATCTTTTGAACTTGCACACTGTTCTCGTTGTCGTATAGATTGGCTTCAAAGAGACGGGATTCTTCTTCTTGAGTTAGATAGATTACTTAGACCTGGAGGTTATTTTGCTTATTCATCTCCAGAAGCATATGCACAGGATGAGGAGGATCTGAGAATATGGAGAGAAATGAGTGACCTTGTGAGTCGTATGTGTTGGAGAATTGCTGCAAAGAGGAACCAGACTGTGATTTGGCAAAAACCCGTAACAAATGACTGTTATCTGCAAAGAGAACCTGGTACTCGTCCTCCACTTTGCCGATCTGACGATGATCCTGATGCTGTTTGGGGTGTGCAGATGGAAGCTTGTATCACTCCCTACTCTGACCATGATCATAAAGCCAAGGGCAGTGGATTAGCTCCTTGGCCAGCTCGATTAACTTCACCTCCTCCACGTCTTCAGGATTTTGGCTATTCAACTGAAATGTTTGAAAAAGACACGGAAATTTGGCGGCGTAGAGTCGAGAGTTACTGGAATCTACTGAGTCCAAAGATCGAATCAGATACTATTAGAAACGTGATGGACATGAAGGCAAATATGGGATCATTTGGAGCTGCTCTGAAGGACAAGGATGTGTGGGTGATGAACGTTGTCCCAGAAGACGGACCGAACACACTGAAGTTGATATACGACCGAGGCCTCATAGGCACTACTAATAACTGGTGTGAAGCCTTCTCGACATATCCTCGTACCTACGATCTGCTCCACGCATGGACCGTCTTTTCCGACGTCGAGAAGAAAGAATGTGGCGCTGAGGACCTGTTGCTCGAGATGGATCGCATGCTCAGACCCACAGGTTTCATCATCATCAGGGACAAGCAATCCGTTGTAGATTTGATAAAGAAGTACCTACCTGCATTGCACTGGGAAACAGTGGCAACCACGGATGCAAGCTCGGATTCAGAATTGGACAGCGACGAGGTCGTGTTTATCGTCCAGAAGAAACTGTGGCTGACAAGTGAGAGTGTGAGGGATTCAGAATAA

Coding sequence (CDS)

ATGACGAGGGGAAGATCTGATGGAACACAGAAAAAGCGCTTAATCACTACCATATGTGTTGTGGCAATATTTCTTGGGTTTCTATATGCATATTATGGATCCATTTTTGGCAATCAAGACCGTGGTGCATCGGCTCTGCAGCATGGCAGCAAATCCTTGAGCCATTATTTGATGGGGAGTGAAGACTCTGAAGAATCTTCAACCTCAACTGAGCAGGAAGATCCAGAAGATGATGTCGTACCAAAAAGTTACCCGGTTTGCGATGACCGGCATTCGGAATTAATTCCCTGCTTGGACAGACATCTCATATATCAAATGAGATTGAAACTGGATCTTTCTTTAATGGAACACTATGAAAGACACTGCCCACCACCTGAGAGGCGGTTCAATTGCCTGATTCCTCCTCCAGCAGGATATAAGGTCCCAATTAAGTGGCCAAAGAGTAGAGATGAAGTGTGGAAAGCTAACATACCTCACACTCATCTTGCACATGAGAAATCTGATCAGAACTGGATGGTTGTCAAAGGTGAAAAAATTGTGTTCCCTGGAGGAGGTACTCATTTTCACTATGGAGCTGATAAGTACATTGCTTCAATTGCCAATATGCTCAACTTTTCAAATGATAATCTAAACAATCAAGGACGATTGCGGACTGTTCTTGATGTTGGTTGTGGTGTTGCAAGTTTTGGAGGATATCTTCTCTCTTCTAATATTATAGCAATGTCCTTAGCACCAAATGATGTTCATCAGAATCAAATTCAGTTTGCTTTGGAAAGAGGAATTCCTGCATACCTTGGTGTTTTAGGAACCAAGAGGCTCCCTTACCCAAGCAGATCTTTTGAACTTGCACACTGTTCTCGTTGTCGTATAGATTGGCTTCAAAGAGACGGGATTCTTCTTCTTGAGTTAGATAGATTACTTAGACCTGGAGGTTATTTTGCTTATTCATCTCCAGAAGCATATGCACAGGATGAGGAGGATCTGAGAATATGGAGAGAAATGAGTGACCTTGTGAGTCGTATGTGTTGGAGAATTGCTGCAAAGAGGAACCAGACTGTGATTTGGCAAAAACCCGTAACAAATGACTGTTATCTGCAAAGAGAACCTGGTACTCGTCCTCCACTTTGCCGATCTGACGATGATCCTGATGCTGTTTGGGGTGTGCAGATGGAAGCTTGTATCACTCCCTACTCTGACCATGATCATAAAGCCAAGGGCAGTGGATTAGCTCCTTGGCCAGCTCGATTAACTTCACCTCCTCCACGTCTTCAGGATTTTGGCTATTCAACTGAAATGTTTGAAAAAGACACGGAAATTTGGCGGCGTAGAGTCGAGAGTTACTGGAATCTACTGAGTCCAAAGATCGAATCAGATACTATTAGAAACGTGATGGACATGAAGGCAAATATGGGATCATTTGGAGCTGCTCTGAAGGACAAGGATGTGTGGGTGATGAACGTTGTCCCAGAAGACGGACCGAACACACTGAAGTTGATATACGACCGAGGCCTCATAGGCACTACTAATAACTGGTGTGAAGCCTTCTCGACATATCCTCGTACCTACGATCTGCTCCACGCATGGACCGTCTTTTCCGACGTCGAGAAGAAAGAATGTGGCGCTGAGGACCTGTTGCTCGAGATGGATCGCATGCTCAGACCCACAGGTTTCATCATCATCAGGGACAAGCAATCCGTTGTAGATTTGATAAAGAAGTACCTACCTGCATTGCACTGGGAAACAGTGGCAACCACGGATGCAAGCTCGGATTCAGAATTGGACAGCGACGAGGTCGTGTTTATCGTCCAGAAGAAACTGTGGCTGACAAGTGAGAGTGTGAGGGATTCAGAATAA

Protein sequence

MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGSEDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYERHCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIVFPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIAMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVTNDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPPPRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDKDVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECGAEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVFIVQKKLWLTSESVRDSE
Homology
BLAST of Lag0016535 vs. NCBI nr
Match: XP_008460648.1 (PREDICTED: probable methyltransferase PMT3 [Cucumis melo] >KAA0065644.1 putative methyltransferase PMT3 [Cucumis melo var. makuwa] >TYK01437.1 putative methyltransferase PMT3 [Cucumis melo var. makuwa])

HSP 1 Score: 1255.7 bits (3248), Expect = 0.0e+00
Identity = 595/617 (96.43%), Postives = 605/617 (98.06%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDGTQKKRLI TICVVAIFLGFLYAYYGSIFGNQD   SALQHGSKSLSHYLMG+
Sbjct: 1   MTRGRSDGTQKKRLIATICVVAIFLGFLYAYYGSIFGNQD---SALQHGSKSLSHYLMGN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEESST+TEQED +DDV+PKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESSTTTEQEDADDDVIPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNN+GRLRTVLDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNEGRLRTVLDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLV RMCWRIAAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVGRMCWRIAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYS EMFEKDTEIWRRRVESYWNLLSPKIE+DTIRNVMDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSNEMFEKDTEIWRRRVESYWNLLSPKIEADTIRNVMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
           +EDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWE VAT DASSDSELD DE +F
Sbjct: 541 SEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWEAVATVDASSDSELDGDEAIF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 614

BLAST of Lag0016535 vs. NCBI nr
Match: XP_023548079.1 (probable methyltransferase PMT3 [Cucurbita pepo subsp. pepo] >XP_023548080.1 probable methyltransferase PMT3 [Cucurbita pepo subsp. pepo] >XP_023548081.1 probable methyltransferase PMT3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1251.5 bits (3237), Expect = 0.0e+00
Identity = 590/617 (95.62%), Postives = 603/617 (97.73%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDGTQKKRLI TICVVAIFLGFLYAYYGSIFGNQDRGA ALQHGSKSLSHYLMG+
Sbjct: 1   MTRGRSDGTQKKRLIATICVVAIFLGFLYAYYGSIFGNQDRGAFALQHGSKSLSHYLMGN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEES+TSTEQ+D ED VVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESTTSTEQDDAEDGVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWR+MSDLV RMCWR+AAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWRKMSDLVGRMCWRVAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHK KGSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKVKGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYS EMFEKDTEIW+RRV SYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSNEMFEKDTEIWQRRVGSYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIG+ NNWCEAFSTYPRTYDLLHAWT+FSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGSINNWCEAFSTYPRTYDLLHAWTIFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
            EDLLLEMDRMLRPTGF+IIRDKQS+VDLIKKYLPALHWETVAT +ASSDSELD DE VF
Sbjct: 541 IEDLLLEMDRMLRPTGFVIIRDKQSIVDLIKKYLPALHWETVATANASSDSELDGDETVF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 617

BLAST of Lag0016535 vs. NCBI nr
Match: XP_022958907.1 (probable methyltransferase PMT3 [Cucurbita moschata] >XP_022958908.1 probable methyltransferase PMT3 [Cucurbita moschata])

HSP 1 Score: 1251.1 bits (3236), Expect = 0.0e+00
Identity = 590/617 (95.62%), Postives = 604/617 (97.89%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDGTQKKRLI TICVVAIFLGFLYAYYGSIFGNQDRGA ALQHGSKSLSHYLMG+
Sbjct: 1   MTRGRSDGTQKKRLIATICVVAIFLGFLYAYYGSIFGNQDRGAFALQHGSKSLSHYLMGN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEES+TSTEQ+D ED VVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESTTSTEQDDAEDGVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRT+LDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTILDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWR+MSDLV RMCWRIAAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWRKMSDLVGRMCWRIAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHK KGSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKVKGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYSTEMFEKDTEIW+RRV SYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSTEMFEKDTEIWQRRVGSYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIG+ NNWCEAFSTYPRTYDLLHAWTVFSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGSINNWCEAFSTYPRTYDLLHAWTVFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
            EDLLLEMDR+LRPTGF+IIRDKQS+VDLIKKYLPALHWET+AT +ASSDSELD DE VF
Sbjct: 541 IEDLLLEMDRILRPTGFVIIRDKQSIVDLIKKYLPALHWETLATANASSDSELDGDETVF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 617

BLAST of Lag0016535 vs. NCBI nr
Match: XP_038874564.1 (probable methyltransferase PMT3 [Benincasa hispida] >XP_038874565.1 probable methyltransferase PMT3 [Benincasa hispida])

HSP 1 Score: 1250.0 bits (3233), Expect = 0.0e+00
Identity = 590/617 (95.62%), Postives = 605/617 (98.06%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDGTQKKRLI TICVVAIFLGFLYAYYGSIFGNQD   SALQHGSKSLSHYLMG+
Sbjct: 1   MTRGRSDGTQKKRLIATICVVAIFLGFLYAYYGSIFGNQD---SALQHGSKSLSHYLMGN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEESST++EQED EDDV+PKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESSTTSEQEDAEDDVIPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNN+GRLRTVLDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNEGRLRTVLDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLV RMCWRIAAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVGRMCWRIAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDD+PDAVWGVQMEACITPYSDHDHKA+GSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDNPDAVWGVQMEACITPYSDHDHKARGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYSTEMFEKD EIWRRRVESYWNLLSPK+E+DTIRN+MDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSTEMFEKDKEIWRRRVESYWNLLSPKMETDTIRNIMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWT+FSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTIFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
           AEDLLLEMDRMLRPTGF+IIRDKQSVVDLIKKYLPALHWE VAT D+SSDSELD DE VF
Sbjct: 541 AEDLLLEMDRMLRPTGFVIIRDKQSVVDLIKKYLPALHWEAVATADSSSDSELDGDEAVF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 614

BLAST of Lag0016535 vs. NCBI nr
Match: XP_023006143.1 (probable methyltransferase PMT3 [Cucurbita maxima] >XP_023006144.1 probable methyltransferase PMT3 [Cucurbita maxima] >XP_023006145.1 probable methyltransferase PMT3 [Cucurbita maxima] >XP_023006147.1 probable methyltransferase PMT3 [Cucurbita maxima])

HSP 1 Score: 1249.2 bits (3231), Expect = 0.0e+00
Identity = 591/617 (95.79%), Postives = 603/617 (97.73%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDGTQKKRLI TICVVAIFLGFLYAYYGSIFG+QD GA ALQHGSKSLSHYLMG+
Sbjct: 1   MTRGRSDGTQKKRLIATICVVAIFLGFLYAYYGSIFGSQDHGAFALQHGSKSLSHYLMGN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEES+TSTEQ+D ED VVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESTTSTEQDDAEDGVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWR+MSDLV RMCWRIAAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWRKMSDLVGRMCWRIAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHK KGSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKVKGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYSTEMFEKDTEIW+RRV SYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSTEMFEKDTEIWQRRVGSYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIG+ NNWCEAFSTYPRTYDLLHAWTVFSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGSINNWCEAFSTYPRTYDLLHAWTVFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
            EDLLLEMDR+LRPTGF+IIRDKQSVVDLIKKYLPALHWETVAT +ASSDSELD DE VF
Sbjct: 541 IEDLLLEMDRILRPTGFVIIRDKQSVVDLIKKYLPALHWETVATANASSDSELDGDETVF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 617

BLAST of Lag0016535 vs. ExPASy Swiss-Prot
Match: Q940J9 (Probable methyltransferase PMT8 OS=Arabidopsis thaliana OX=3702 GN=At1g04430 PE=2 SV=1)

HSP 1 Score: 1049.7 bits (2713), Expect = 1.3e-305
Identity = 496/627 (79.11%), Postives = 554/627 (88.36%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHG---SKSLSHYL 60
           M RGRSDG  KKRLI ++CVVA+F+ FL+ YYGS      +GASAL++G    K  S YL
Sbjct: 1   MMRGRSDGGLKKRLIASVCVVALFVCFLFMYYGS----SSQGASALEYGRSLRKLGSSYL 60

Query: 61  MGSEDSEESSTSTEQEDPEDD-VVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLME 120
            G +D+ ++       + ED  VV KS+PVCDDRHSE+IPCLDR+ IYQMRLKLDLSLME
Sbjct: 61  SGDDDNGDTKQDDSVANAEDSLVVAKSFPVCDDRHSEIIPCLDRNFIYQMRLKLDLSLME 120

Query: 121 HYERHCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKG 180
           HYERHCPPPERRFNCLIPPP+GYKVPIKWPKSRDEVWKANIPHTHLA EKSDQNWMV KG
Sbjct: 121 HYERHCPPPERRFNCLIPPPSGYKVPIKWPKSRDEVWKANIPHTHLAKEKSDQNWMVEKG 180

Query: 181 EKIVFPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSS 240
           EKI FPGGGTHFHYGADKYIASIANMLNFSND LN++GRLRTVLDVGCGVASFG YLL+S
Sbjct: 181 EKISFPGGGTHFHYGADKYIASIANMLNFSNDVLNDEGRLRTVLDVGCGVASFGAYLLAS 240

Query: 241 NIIAMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRD 300
           +I+ MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFE AHCSRCRIDWLQRD
Sbjct: 241 DIMTMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFEFAHCSRCRIDWLQRD 300

Query: 301 GILLLELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQ 360
           G+LLLELDR+LRPGGYFAYSSPEAYAQDEE+L+IW+EMS LV RMCWRIA KRNQTV+WQ
Sbjct: 301 GLLLLELDRVLRPGGYFAYSSPEAYAQDEENLKIWKEMSALVERMCWRIAVKRNQTVVWQ 360

Query: 361 KPVTNDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARL 420
           KP++NDCYL+REPGT+PPLCRSD DPDAV GV MEACITPYS HDHK KGSGLAPWPARL
Sbjct: 361 KPLSNDCYLEREPGTQPPLCRSDADPDAVAGVSMEACITPYSKHDHKTKGSGLAPWPARL 420

Query: 421 TSPPPRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAA 480
           TS PPRL DFGYST+MFEKDTE+W+++V+SYWNL+S K++S+T+RN+MDMKA+MGSF AA
Sbjct: 421 TSSPPRLADFGYSTDMFEKDTELWKQQVDSYWNLMSSKVKSNTVRNIMDMKAHMGSFAAA 480

Query: 481 LKDKDVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEK 540
           LKDKDVWVMNVV  DGPNTLKLIYDRGLIGT +NWCEAFSTYPRTYDLLHAW++FSD++ 
Sbjct: 481 LKDKDVWVMNVVSPDGPNTLKLIYDRGLIGTNHNWCEAFSTYPRTYDLLHAWSIFSDIKS 540

Query: 541 KECGAEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSD 600
           K C AEDLL+EMDR+LRPTGF+IIRDKQSVV+ IKKYL ALHWETVA+   ++ SELD D
Sbjct: 541 KGCSAEDLLIEMDRILRPTGFVIIRDKQSVVESIKKYLQALHWETVASEKVNTSSELDQD 600

Query: 601 ------EVVFIVQKKLWLTSESVRDSE 618
                  VVFIVQKKLWLTSES+RDSE
Sbjct: 601 SEDGENNVVFIVQKKLWLTSESLRDSE 623

BLAST of Lag0016535 vs. ExPASy Swiss-Prot
Match: Q93YV7 (Probable methyltransferase PMT3 OS=Arabidopsis thaliana OX=3702 GN=At4g14360 PE=2 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 1.7e-305
Identity = 492/615 (80.00%), Postives = 543/615 (88.29%), Query Frame = 0

Query: 3   RGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGSED 62
           +GRSDG QKKR+I  +CV A+ L F+Y +YGS     D  ASA+++G K     L G +D
Sbjct: 2   KGRSDGGQKKRVIALVCVAAVVLVFVYLFYGS----SDHRASAIEYGRKL---GLGGDDD 61

Query: 63  SEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYERHC 122
             +   ++     +D   P+S+PVCDDRHSELIPCLDR+LIYQMRLKLDLSLMEHYERHC
Sbjct: 62  DTKQDDTSSSFGVDDGFTPRSFPVCDDRHSELIPCLDRNLIYQMRLKLDLSLMEHYERHC 121

Query: 123 PPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIVFP 182
           PPPERRFNCLIPPP GYKVPIKWPKSRDEVWK NIPHTHLAHEKSDQNWMVVKG+KI FP
Sbjct: 122 PPPERRFNCLIPPPNGYKVPIKWPKSRDEVWKVNIPHTHLAHEKSDQNWMVVKGDKINFP 181

Query: 183 GGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIAMS 242
           GGGTHFHYGADKYIAS+ANMLN+ N+ LNN GRLRTV DVGCGVASFGGYLLSS+I+ MS
Sbjct: 182 GGGTHFHYGADKYIASMANMLNYPNNVLNNGGRLRTVFDVGCGVASFGGYLLSSDILTMS 241

Query: 243 LAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLE 302
           LAPNDVHQNQIQFALERGIPA LGVLGTKRLPYPSRSFEL+HCSRCRIDWLQRDGILLLE
Sbjct: 242 LAPNDVHQNQIQFALERGIPASLGVLGTKRLPYPSRSFELSHCSRCRIDWLQRDGILLLE 301

Query: 303 LDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVTND 362
           LDR+LRPGGYFAYSSPEAYAQDEEDLRIWREMS LV RMCW+IAAKRNQTVIWQKP+TND
Sbjct: 302 LDRVLRPGGYFAYSSPEAYAQDEEDLRIWREMSALVERMCWKIAAKRNQTVIWQKPLTND 361

Query: 363 CYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPPPR 422
           CYL+REPGT+PPLCRSD+DPDAVWGV MEACIT YSDHDHK KGSGLAPWPARLTSPPPR
Sbjct: 362 CYLEREPGTQPPLCRSDNDPDAVWGVNMEACITSYSDHDHKTKGSGLAPWPARLTSPPPR 421

Query: 423 LQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDKDV 482
           L DFGYST MFEKDTE+WR+RV++YW+LLSP+IESDT+RN+MDMKA+MGSF AALK+KDV
Sbjct: 422 LADFGYSTGMFEKDTELWRQRVDTYWDLLSPRIESDTVRNIMDMKASMGSFAAALKEKDV 481

Query: 483 WVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECGAE 542
           WVMNVVPEDGPNTLKLIYDRGL+G  ++WCEAFSTYPRTYDLLHAW + SD++KK C   
Sbjct: 482 WVMNVVPEDGPNTLKLIYDRGLMGAVHSWCEAFSTYPRTYDLLHAWDIISDIKKKGCSEV 541

Query: 543 DLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVFIV 602
           DLLLEMDR+LRP+GFIIIRDKQ VVD +KKYL ALHWE V  T   SDS+ DSD VVFIV
Sbjct: 542 DLLLEMDRILRPSGFIIIRDKQRVVDFVKKYLKALHWEEVG-TKTDSDSDQDSDNVVFIV 601

Query: 603 QKKLWLTSESVRDSE 618
           QKKLWLTSES+RD E
Sbjct: 602 QKKLWLTSESLRDME 608

BLAST of Lag0016535 vs. ExPASy Swiss-Prot
Match: Q8H118 (Probable methyltransferase PMT1 OS=Arabidopsis thaliana OX=3702 GN=At3g23300 PE=2 SV=2)

HSP 1 Score: 1029.6 bits (2661), Expect = 1.4e-299
Identity = 487/620 (78.55%), Postives = 539/620 (86.94%), Query Frame = 0

Query: 3   RGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGSED 62
           RGRS+G +KK +I  +CV ++ L F+Y ++GS          A+++G K     L G +D
Sbjct: 2   RGRSEGGKKKPVIVLLCVASVVLVFVYLFFGS------SNHKAIEYGRKL---GLGGDDD 61

Query: 63  SEESSTSTEQEDPEDDVV-----PKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEH 122
                  T      +DVV     P+S+PVCDDRHSELIPCLDR+LIYQMRLKLDLSLMEH
Sbjct: 62  DSTKKDDTSSSFYVEDVVGNGFTPRSFPVCDDRHSELIPCLDRNLIYQMRLKLDLSLMEH 121

Query: 123 YERHCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGE 182
           YERHCPPPERRFNCLIPPP GYK+PIKWPKSRDEVWK NIPHTHLAHEKSDQNWMVVKGE
Sbjct: 122 YERHCPPPERRFNCLIPPPPGYKIPIKWPKSRDEVWKVNIPHTHLAHEKSDQNWMVVKGE 181

Query: 183 KIVFPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSN 242
           KI FPGGGTHFHYGADKYIAS+ANMLNF N+ LNN GRLRT LDVGCGVASFGGYLL+S 
Sbjct: 182 KINFPGGGTHFHYGADKYIASMANMLNFPNNVLNNGGRLRTFLDVGCGVASFGGYLLASE 241

Query: 243 IIAMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDG 302
           I+ MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDG
Sbjct: 242 IMTMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDG 301

Query: 303 ILLLELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQK 362
           ILLLELDR+LRPGGYFAYSSPEAYAQDEEDLRIWREMS LV RMCW IAAKRNQTVIWQK
Sbjct: 302 ILLLELDRVLRPGGYFAYSSPEAYAQDEEDLRIWREMSALVGRMCWTIAAKRNQTVIWQK 361

Query: 363 PVTNDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLT 422
           P+TNDCYL REPGT+PPLC SD DPDAV+GV MEACIT YSDHDHK KGSGLAPWPARLT
Sbjct: 362 PLTNDCYLGREPGTQPPLCNSDSDPDAVYGVNMEACITQYSDHDHKTKGSGLAPWPARLT 421

Query: 423 SPPPRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAAL 482
           SPPPRL DFGYST++FEKDTE WR+RV++YW+LLSPKI+SDT+RN+MDMKA+MGSF AAL
Sbjct: 422 SPPPRLADFGYSTDIFEKDTETWRQRVDTYWDLLSPKIQSDTVRNIMDMKASMGSFAAAL 481

Query: 483 KDKDVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKK 542
           K+KDVWVMNVVPEDGPNTLKLIYDRGL+G  ++WCEAFSTYPRTYDLLHAW + SD++K+
Sbjct: 482 KEKDVWVMNVVPEDGPNTLKLIYDRGLMGAVHSWCEAFSTYPRTYDLLHAWDIISDIKKR 541

Query: 543 ECGAEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDE 602
            C AEDLLLEMDR+LRP+GFI+IRDKQSVVDL+KKYL ALHWE V T  A S+S+ DSD 
Sbjct: 542 GCSAEDLLLEMDRILRPSGFILIRDKQSVVDLVKKYLKALHWEAVETKTA-SESDQDSDN 601

Query: 603 VVFIVQKKLWLTSESVRDSE 618
           V+ IVQKKLWLTSES+RD E
Sbjct: 602 VILIVQKKLWLTSESLRDLE 611

BLAST of Lag0016535 vs. ExPASy Swiss-Prot
Match: Q8VZV7 (Probable methyltransferase PMT9 OS=Arabidopsis thaliana OX=3702 GN=At5g14430 PE=2 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 6.1e-234
Identity = 382/597 (63.99%), Postives = 449/597 (75.21%), Query Frame = 0

Query: 13  RLITTICVVAI-FLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGSEDSEESSTSTE 72
           +L T + V  I  LG    YYGS F    R +     GS +     +GS  + +   +  
Sbjct: 14  KLFTYVLVGFIALLGLTCLYYGSSFAPGSRKSDEFD-GSNNRVRTGIGSLRNRDIVLAVS 73

Query: 73  QEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYERHCPPPERRFNC 132
           + +     VPKS P+CD RHSELIPCLDR+L YQ++LKL+LSLMEHYE HCPP ERRFNC
Sbjct: 74  RFE-----VPKSVPICDSRHSELIPCLDRNLHYQLKLKLNLSLMEHYEHHCPPSERRFNC 133

Query: 133 LIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIVFPGGGTHFHYG 192
           L+PPP GYK+P++WP SRDEVWKANIPHTHLA EKSDQNWMVV G+KI FPGGGTHFH G
Sbjct: 134 LVPPPVGYKIPLRWPVSRDEVWKANIPHTHLAQEKSDQNWMVVNGDKINFPGGGTHFHNG 193

Query: 193 ADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIAMSLAPNDVHQN 252
           ADKYI S+A ML F  D LNN G +R VLDVGCGVASFG YLLS +IIAMSLAPNDVHQN
Sbjct: 194 ADKYIVSLAQMLKFPGDKLNNGGSIRNVLDVGCGVASFGAYLLSHDIIAMSLAPNDVHQN 253

Query: 253 QIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLELDRLLRPGG 312
           QIQFALERGIP+ LGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLELDRLLRPGG
Sbjct: 254 QIQFALERGIPSTLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLELDRLLRPGG 313

Query: 313 YFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVTNDCYLQREPGT 372
           YF YSSPEAYA D E+ +I   M DL  RMCW++ AKR+Q+VIW KP++N CYL+R+PG 
Sbjct: 314 YFVYSSPEAYAHDPENRKIGNAMHDLFKRMCWKVVAKRDQSVIWGKPISNSCYLKRDPGV 373

Query: 373 RPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPPPRLQDFGYSTE 432
            PPLC S DDPDA W V M+ACI+PYS   HK + SGL PWP RLT+PPPRL++ G + E
Sbjct: 374 LPPLCPSGDDPDATWNVSMKACISPYSVRMHKERWSGLVPWPRRLTAPPPRLEEIGVTPE 433

Query: 433 MFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDKDVWVMNVVPED 492
            F +DTE WR RV  YW LL P ++ ++IRNVMDM +N+G F AAL DKDVWVMNV+P  
Sbjct: 434 QFREDTETWRLRVIEYWKLLKPMVQKNSIRNVMDMSSNLGGFAAALNDKDVWVMNVMPVQ 493

Query: 493 GPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECGAEDLLLEMDRM 552
               +K+IYDRGLIG T++WCEAF TYPRT+DL+HAW  F++ + + C  EDLL+EMDR+
Sbjct: 494 SSPRMKIIYDRGLIGATHDWCEAFDTYPRTFDLIHAWNTFTETQARGCSFEDLLIEMDRI 553

Query: 553 LRPTGFIIIRDKQSVVDLIKKYLPALHWETVAT-TDASSDSELDSDEVVFIVQKKLW 608
           LRP GF+IIRD    +  IKKYL  L W+  +T T    D     DE+V I +KKLW
Sbjct: 554 LRPEGFVIIRDTTDNISYIKKYLTLLKWDKWSTETTPKGDPLSTKDEIVLIARKKLW 604

BLAST of Lag0016535 vs. ExPASy Swiss-Prot
Match: Q94KE1 (Probable methyltransferase PMT10 OS=Arabidopsis thaliana OX=3702 GN=At1g77260 PE=2 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.1e-145
Identity = 247/521 (47.41%), Postives = 339/521 (65.07%), Query Frame = 0

Query: 66  SSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYERHCPPP 125
           +S+  E+E PE     +   +CD    + IPCLD     +++   +    E+YERHC  P
Sbjct: 125 NSSVEEKESPEVGFQIEKLKLCDKTKIDYIPCLDNE--EEIKRLNNTDRGENYERHC--P 184

Query: 126 ERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIVFPGGG 185
           ++  +CLIPPP GYK PI+WP+SRD++W  N+PHT L  +K  QNW+  + +K VFPGGG
Sbjct: 185 KQSLDCLIPPPDGYKKPIQWPQSRDKIWFNNVPHTRLVEDKGGQNWIRREKDKFVFPGGG 244

Query: 186 THFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIAMSLAP 245
           T F +GAD+Y+  I+ M+     ++    R R  LD+GCGVASFG +L+  N   +S+AP
Sbjct: 245 TQFIHGADQYLDQISQMI----PDITFGSRTRVALDIGCGVASFGAFLMQRNTTTLSVAP 304

Query: 246 NDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLELDR 305
            DVH+NQIQFALERG+PA + V  T+RL YPS+SFE+ HCSRCRI+W + DGILLLE++R
Sbjct: 305 KDVHENQIQFALERGVPAMVAVFATRRLLYPSQSFEMIHCSRCRINWTRDDGILLLEVNR 364

Query: 306 LLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVTNDCYL 365
           +LR GGYF +++   Y  ++     W+EM DL +R+CW +  K     +W+KP+ N CY+
Sbjct: 365 MLRAGGYFVWAAQPVYKHEDNLQEQWKEMLDLTNRICWELIKKEGYIAVWRKPLNNSCYV 424

Query: 366 QREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPPPRLQD 425
            RE GT+PPLCR DDDPD VW V M+ CIT   D+ +   G+ ++ WPARL  PP RLQ 
Sbjct: 425 SREAGTKPPLCRPDDDPDDVWYVDMKPCITRLPDNGY---GANVSTWPARLHDPPERLQS 484

Query: 426 FGYST-----EMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKD- 485
                     E+ + ++  W   VESY  +   + +   +RNV+DM+A  G F AAL D 
Sbjct: 485 IQMDAYISRKEIMKAESRFWLEVVESYVRVF--RWKEFKLRNVLDMRAGFGGFAAALNDL 544

Query: 486 -KDVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKE 545
             D WVMN+VP  G NTL +IYDRGL G  ++WCE F TYPRTYDL+HA  +FS VEKK 
Sbjct: 545 GLDCWVMNIVPVSGFNTLPVIYDRGLQGAMHDWCEPFDTYPRTYDLIHAAFLFS-VEKKR 604

Query: 546 CGAEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHW 580
           C   +++LEMDRMLRP G + IRD  S++D +++   A+ W
Sbjct: 605 CNITNIMLEMDRMLRPGGHVYIRDSLSLMDQLQQVAKAIGW 631

BLAST of Lag0016535 vs. ExPASy TrEMBL
Match: A0A5D3BQ04 (Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold772G00220 PE=3 SV=1)

HSP 1 Score: 1255.7 bits (3248), Expect = 0.0e+00
Identity = 595/617 (96.43%), Postives = 605/617 (98.06%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDGTQKKRLI TICVVAIFLGFLYAYYGSIFGNQD   SALQHGSKSLSHYLMG+
Sbjct: 1   MTRGRSDGTQKKRLIATICVVAIFLGFLYAYYGSIFGNQD---SALQHGSKSLSHYLMGN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEESST+TEQED +DDV+PKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESSTTTEQEDADDDVIPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNN+GRLRTVLDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNEGRLRTVLDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLV RMCWRIAAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVGRMCWRIAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYS EMFEKDTEIWRRRVESYWNLLSPKIE+DTIRNVMDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSNEMFEKDTEIWRRRVESYWNLLSPKIEADTIRNVMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
           +EDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWE VAT DASSDSELD DE +F
Sbjct: 541 SEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWEAVATVDASSDSELDGDEAIF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 614

BLAST of Lag0016535 vs. ExPASy TrEMBL
Match: A0A1S3CDD7 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499424 PE=3 SV=1)

HSP 1 Score: 1255.7 bits (3248), Expect = 0.0e+00
Identity = 595/617 (96.43%), Postives = 605/617 (98.06%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDGTQKKRLI TICVVAIFLGFLYAYYGSIFGNQD   SALQHGSKSLSHYLMG+
Sbjct: 1   MTRGRSDGTQKKRLIATICVVAIFLGFLYAYYGSIFGNQD---SALQHGSKSLSHYLMGN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEESST+TEQED +DDV+PKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESSTTTEQEDADDDVIPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNN+GRLRTVLDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNEGRLRTVLDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLV RMCWRIAAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVGRMCWRIAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYS EMFEKDTEIWRRRVESYWNLLSPKIE+DTIRNVMDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSNEMFEKDTEIWRRRVESYWNLLSPKIEADTIRNVMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
           +EDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWE VAT DASSDSELD DE +F
Sbjct: 541 SEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWEAVATVDASSDSELDGDEAIF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 614

BLAST of Lag0016535 vs. ExPASy TrEMBL
Match: A0A6J1H346 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460059 PE=3 SV=1)

HSP 1 Score: 1251.1 bits (3236), Expect = 0.0e+00
Identity = 590/617 (95.62%), Postives = 604/617 (97.89%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDGTQKKRLI TICVVAIFLGFLYAYYGSIFGNQDRGA ALQHGSKSLSHYLMG+
Sbjct: 1   MTRGRSDGTQKKRLIATICVVAIFLGFLYAYYGSIFGNQDRGAFALQHGSKSLSHYLMGN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEES+TSTEQ+D ED VVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESTTSTEQDDAEDGVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRT+LDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTILDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWR+MSDLV RMCWRIAAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWRKMSDLVGRMCWRIAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHK KGSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKVKGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYSTEMFEKDTEIW+RRV SYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSTEMFEKDTEIWQRRVGSYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIG+ NNWCEAFSTYPRTYDLLHAWTVFSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGSINNWCEAFSTYPRTYDLLHAWTVFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
            EDLLLEMDR+LRPTGF+IIRDKQS+VDLIKKYLPALHWET+AT +ASSDSELD DE VF
Sbjct: 541 IEDLLLEMDRILRPTGFVIIRDKQSIVDLIKKYLPALHWETLATANASSDSELDGDETVF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 617

BLAST of Lag0016535 vs. ExPASy TrEMBL
Match: A0A6J1KZD4 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111498974 PE=3 SV=1)

HSP 1 Score: 1249.2 bits (3231), Expect = 0.0e+00
Identity = 591/617 (95.79%), Postives = 603/617 (97.73%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDGTQKKRLI TICVVAIFLGFLYAYYGSIFG+QD GA ALQHGSKSLSHYLMG+
Sbjct: 1   MTRGRSDGTQKKRLIATICVVAIFLGFLYAYYGSIFGSQDHGAFALQHGSKSLSHYLMGN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEES+TSTEQ+D ED VVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESTTSTEQDDAEDGVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWR+MSDLV RMCWRIAAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWRKMSDLVGRMCWRIAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHK KGSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKVKGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYSTEMFEKDTEIW+RRV SYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSTEMFEKDTEIWQRRVGSYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIG+ NNWCEAFSTYPRTYDLLHAWTVFSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGSINNWCEAFSTYPRTYDLLHAWTVFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
            EDLLLEMDR+LRPTGF+IIRDKQSVVDLIKKYLPALHWETVAT +ASSDSELD DE VF
Sbjct: 541 IEDLLLEMDRILRPTGFVIIRDKQSVVDLIKKYLPALHWETVATANASSDSELDGDETVF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 617

BLAST of Lag0016535 vs. ExPASy TrEMBL
Match: A0A0A0LI73 (Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G174120 PE=3 SV=1)

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 590/617 (95.62%), Postives = 604/617 (97.89%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGS 60
           MTRGRSDG+QKKRLI TICVVAIFLGFLYAYYGSIFGNQD   SALQHGSKSLSHYLM +
Sbjct: 1   MTRGRSDGSQKKRLIATICVVAIFLGFLYAYYGSIFGNQD---SALQHGSKSLSHYLMRN 60

Query: 61  EDSEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120
           EDSEESST+TEQED +DDV+PKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER
Sbjct: 61  EDSEESSTTTEQEDADDDVIPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYER 120

Query: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180
           HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV
Sbjct: 121 HCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIV 180

Query: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIA 240
           FPGGGTHFHYGADKYIASIANMLNFSNDNLNN+GRLRTVLDVGCGVASFGGYLLSSNIIA
Sbjct: 181 FPGGGTHFHYGADKYIASIANMLNFSNDNLNNEGRLRTVLDVGCGVASFGGYLLSSNIIA 240

Query: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300
           MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL
Sbjct: 241 MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILL 300

Query: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVT 360
           LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLV RMCWRIAAKRNQTVIWQKP+T
Sbjct: 301 LELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVGRMCWRIAAKRNQTVIWQKPLT 360

Query: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPP 420
           NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACI+PYSD DHKAKGSGLAPWPARLTSPP
Sbjct: 361 NDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACISPYSDRDHKAKGSGLAPWPARLTSPP 420

Query: 421 PRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDK 480
           PRLQDFGYS EMFEKDTE+WRRRVESYWNLLSPKIE+DTIRNVMDMKANMGSFGAALKDK
Sbjct: 421 PRLQDFGYSNEMFEKDTEMWRRRVESYWNLLSPKIETDTIRNVMDMKANMGSFGAALKDK 480

Query: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECG 540
           DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSD+EKKEC 
Sbjct: 481 DVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDIEKKECS 540

Query: 541 AEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVF 600
           +EDLLLEMDRMLRPTGFIIIRDKQSV+DLIKKYLPALHWE VAT DASSDSELDSDE +F
Sbjct: 541 SEDLLLEMDRMLRPTGFIIIRDKQSVIDLIKKYLPALHWEAVATADASSDSELDSDEAIF 600

Query: 601 IVQKKLWLTSESVRDSE 618
           IVQKKLWLTSESVRDSE
Sbjct: 601 IVQKKLWLTSESVRDSE 614

BLAST of Lag0016535 vs. TAIR 10
Match: AT1G04430.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 1049.7 bits (2713), Expect = 9.4e-307
Identity = 496/627 (79.11%), Postives = 554/627 (88.36%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHG---SKSLSHYL 60
           M RGRSDG  KKRLI ++CVVA+F+ FL+ YYGS      +GASAL++G    K  S YL
Sbjct: 1   MMRGRSDGGLKKRLIASVCVVALFVCFLFMYYGS----SSQGASALEYGRSLRKLGSSYL 60

Query: 61  MGSEDSEESSTSTEQEDPEDD-VVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLME 120
            G +D+ ++       + ED  VV KS+PVCDDRHSE+IPCLDR+ IYQMRLKLDLSLME
Sbjct: 61  SGDDDNGDTKQDDSVANAEDSLVVAKSFPVCDDRHSEIIPCLDRNFIYQMRLKLDLSLME 120

Query: 121 HYERHCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKG 180
           HYERHCPPPERRFNCLIPPP+GYKVPIKWPKSRDEVWKANIPHTHLA EKSDQNWMV KG
Sbjct: 121 HYERHCPPPERRFNCLIPPPSGYKVPIKWPKSRDEVWKANIPHTHLAKEKSDQNWMVEKG 180

Query: 181 EKIVFPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSS 240
           EKI FPGGGTHFHYGADKYIASIANMLNFSND LN++GRLRTVLDVGCGVASFG YLL+S
Sbjct: 181 EKISFPGGGTHFHYGADKYIASIANMLNFSNDVLNDEGRLRTVLDVGCGVASFGAYLLAS 240

Query: 241 NIIAMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRD 300
           +I+ MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFE AHCSRCRIDWLQRD
Sbjct: 241 DIMTMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFEFAHCSRCRIDWLQRD 300

Query: 301 GILLLELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQ 360
           G+LLLELDR+LRPGGYFAYSSPEAYAQDEE+L+IW+EMS LV RMCWRIA KRNQTV+WQ
Sbjct: 301 GLLLLELDRVLRPGGYFAYSSPEAYAQDEENLKIWKEMSALVERMCWRIAVKRNQTVVWQ 360

Query: 361 KPVTNDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARL 420
           KP++NDCYL+REPGT+PPLCRSD DPDAV GV MEACITPYS HDHK KGSGLAPWPARL
Sbjct: 361 KPLSNDCYLEREPGTQPPLCRSDADPDAVAGVSMEACITPYSKHDHKTKGSGLAPWPARL 420

Query: 421 TSPPPRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAA 480
           TS PPRL DFGYST+MFEKDTE+W+++V+SYWNL+S K++S+T+RN+MDMKA+MGSF AA
Sbjct: 421 TSSPPRLADFGYSTDMFEKDTELWKQQVDSYWNLMSSKVKSNTVRNIMDMKAHMGSFAAA 480

Query: 481 LKDKDVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEK 540
           LKDKDVWVMNVV  DGPNTLKLIYDRGLIGT +NWCEAFSTYPRTYDLLHAW++FSD++ 
Sbjct: 481 LKDKDVWVMNVVSPDGPNTLKLIYDRGLIGTNHNWCEAFSTYPRTYDLLHAWSIFSDIKS 540

Query: 541 KECGAEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSD 600
           K C AEDLL+EMDR+LRPTGF+IIRDKQSVV+ IKKYL ALHWETVA+   ++ SELD D
Sbjct: 541 KGCSAEDLLIEMDRILRPTGFVIIRDKQSVVESIKKYLQALHWETVASEKVNTSSELDQD 600

Query: 601 ------EVVFIVQKKLWLTSESVRDSE 618
                  VVFIVQKKLWLTSES+RDSE
Sbjct: 601 SEDGENNVVFIVQKKLWLTSESLRDSE 623

BLAST of Lag0016535 vs. TAIR 10
Match: AT1G04430.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 1049.7 bits (2713), Expect = 9.4e-307
Identity = 496/627 (79.11%), Postives = 554/627 (88.36%), Query Frame = 0

Query: 1   MTRGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHG---SKSLSHYL 60
           M RGRSDG  KKRLI ++CVVA+F+ FL+ YYGS      +GASAL++G    K  S YL
Sbjct: 1   MMRGRSDGGLKKRLIASVCVVALFVCFLFMYYGS----SSQGASALEYGRSLRKLGSSYL 60

Query: 61  MGSEDSEESSTSTEQEDPEDD-VVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLME 120
            G +D+ ++       + ED  VV KS+PVCDDRHSE+IPCLDR+ IYQMRLKLDLSLME
Sbjct: 61  SGDDDNGDTKQDDSVANAEDSLVVAKSFPVCDDRHSEIIPCLDRNFIYQMRLKLDLSLME 120

Query: 121 HYERHCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKG 180
           HYERHCPPPERRFNCLIPPP+GYKVPIKWPKSRDEVWKANIPHTHLA EKSDQNWMV KG
Sbjct: 121 HYERHCPPPERRFNCLIPPPSGYKVPIKWPKSRDEVWKANIPHTHLAKEKSDQNWMVEKG 180

Query: 181 EKIVFPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSS 240
           EKI FPGGGTHFHYGADKYIASIANMLNFSND LN++GRLRTVLDVGCGVASFG YLL+S
Sbjct: 181 EKISFPGGGTHFHYGADKYIASIANMLNFSNDVLNDEGRLRTVLDVGCGVASFGAYLLAS 240

Query: 241 NIIAMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRD 300
           +I+ MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFE AHCSRCRIDWLQRD
Sbjct: 241 DIMTMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFEFAHCSRCRIDWLQRD 300

Query: 301 GILLLELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQ 360
           G+LLLELDR+LRPGGYFAYSSPEAYAQDEE+L+IW+EMS LV RMCWRIA KRNQTV+WQ
Sbjct: 301 GLLLLELDRVLRPGGYFAYSSPEAYAQDEENLKIWKEMSALVERMCWRIAVKRNQTVVWQ 360

Query: 361 KPVTNDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARL 420
           KP++NDCYL+REPGT+PPLCRSD DPDAV GV MEACITPYS HDHK KGSGLAPWPARL
Sbjct: 361 KPLSNDCYLEREPGTQPPLCRSDADPDAVAGVSMEACITPYSKHDHKTKGSGLAPWPARL 420

Query: 421 TSPPPRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAA 480
           TS PPRL DFGYST+MFEKDTE+W+++V+SYWNL+S K++S+T+RN+MDMKA+MGSF AA
Sbjct: 421 TSSPPRLADFGYSTDMFEKDTELWKQQVDSYWNLMSSKVKSNTVRNIMDMKAHMGSFAAA 480

Query: 481 LKDKDVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEK 540
           LKDKDVWVMNVV  DGPNTLKLIYDRGLIGT +NWCEAFSTYPRTYDLLHAW++FSD++ 
Sbjct: 481 LKDKDVWVMNVVSPDGPNTLKLIYDRGLIGTNHNWCEAFSTYPRTYDLLHAWSIFSDIKS 540

Query: 541 KECGAEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSD 600
           K C AEDLL+EMDR+LRPTGF+IIRDKQSVV+ IKKYL ALHWETVA+   ++ SELD D
Sbjct: 541 KGCSAEDLLIEMDRILRPTGFVIIRDKQSVVESIKKYLQALHWETVASEKVNTSSELDQD 600

Query: 601 ------EVVFIVQKKLWLTSESVRDSE 618
                  VVFIVQKKLWLTSES+RDSE
Sbjct: 601 SEDGENNVVFIVQKKLWLTSESLRDSE 623

BLAST of Lag0016535 vs. TAIR 10
Match: AT4G14360.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 1049.3 bits (2712), Expect = 1.2e-306
Identity = 492/615 (80.00%), Postives = 543/615 (88.29%), Query Frame = 0

Query: 3   RGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGSED 62
           +GRSDG QKKR+I  +CV A+ L F+Y +YGS     D  ASA+++G K     L G +D
Sbjct: 2   KGRSDGGQKKRVIALVCVAAVVLVFVYLFYGS----SDHRASAIEYGRKL---GLGGDDD 61

Query: 63  SEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYERHC 122
             +   ++     +D   P+S+PVCDDRHSELIPCLDR+LIYQMRLKLDLSLMEHYERHC
Sbjct: 62  DTKQDDTSSSFGVDDGFTPRSFPVCDDRHSELIPCLDRNLIYQMRLKLDLSLMEHYERHC 121

Query: 123 PPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIVFP 182
           PPPERRFNCLIPPP GYKVPIKWPKSRDEVWK NIPHTHLAHEKSDQNWMVVKG+KI FP
Sbjct: 122 PPPERRFNCLIPPPNGYKVPIKWPKSRDEVWKVNIPHTHLAHEKSDQNWMVVKGDKINFP 181

Query: 183 GGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIAMS 242
           GGGTHFHYGADKYIAS+ANMLN+ N+ LNN GRLRTV DVGCGVASFGGYLLSS+I+ MS
Sbjct: 182 GGGTHFHYGADKYIASMANMLNYPNNVLNNGGRLRTVFDVGCGVASFGGYLLSSDILTMS 241

Query: 243 LAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLE 302
           LAPNDVHQNQIQFALERGIPA LGVLGTKRLPYPSRSFEL+HCSRCRIDWLQRDGILLLE
Sbjct: 242 LAPNDVHQNQIQFALERGIPASLGVLGTKRLPYPSRSFELSHCSRCRIDWLQRDGILLLE 301

Query: 303 LDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVTND 362
           LDR+LRPGGYFAYSSPEAYAQDEEDLRIWREMS LV RMCW+IAAKRNQTVIWQKP+TND
Sbjct: 302 LDRVLRPGGYFAYSSPEAYAQDEEDLRIWREMSALVERMCWKIAAKRNQTVIWQKPLTND 361

Query: 363 CYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPPPR 422
           CYL+REPGT+PPLCRSD+DPDAVWGV MEACIT YSDHDHK KGSGLAPWPARLTSPPPR
Sbjct: 362 CYLEREPGTQPPLCRSDNDPDAVWGVNMEACITSYSDHDHKTKGSGLAPWPARLTSPPPR 421

Query: 423 LQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDKDV 482
           L DFGYST MFEKDTE+WR+RV++YW+LLSP+IESDT+RN+MDMKA+MGSF AALK+KDV
Sbjct: 422 LADFGYSTGMFEKDTELWRQRVDTYWDLLSPRIESDTVRNIMDMKASMGSFAAALKEKDV 481

Query: 483 WVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECGAE 542
           WVMNVVPEDGPNTLKLIYDRGL+G  ++WCEAFSTYPRTYDLLHAW + SD++KK C   
Sbjct: 482 WVMNVVPEDGPNTLKLIYDRGLMGAVHSWCEAFSTYPRTYDLLHAWDIISDIKKKGCSEV 541

Query: 543 DLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVFIV 602
           DLLLEMDR+LRP+GFIIIRDKQ VVD +KKYL ALHWE V  T   SDS+ DSD VVFIV
Sbjct: 542 DLLLEMDRILRPSGFIIIRDKQRVVDFVKKYLKALHWEEVG-TKTDSDSDQDSDNVVFIV 601

Query: 603 QKKLWLTSESVRDSE 618
           QKKLWLTSES+RD E
Sbjct: 602 QKKLWLTSESLRDME 608

BLAST of Lag0016535 vs. TAIR 10
Match: AT4G14360.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 1049.3 bits (2712), Expect = 1.2e-306
Identity = 492/615 (80.00%), Postives = 543/615 (88.29%), Query Frame = 0

Query: 3   RGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGSED 62
           +GRSDG QKKR+I  +CV A+ L F+Y +YGS     D  ASA+++G K     L G +D
Sbjct: 2   KGRSDGGQKKRVIALVCVAAVVLVFVYLFYGS----SDHRASAIEYGRKL---GLGGDDD 61

Query: 63  SEESSTSTEQEDPEDDVVPKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEHYERHC 122
             +   ++     +D   P+S+PVCDDRHSELIPCLDR+LIYQMRLKLDLSLMEHYERHC
Sbjct: 62  DTKQDDTSSSFGVDDGFTPRSFPVCDDRHSELIPCLDRNLIYQMRLKLDLSLMEHYERHC 121

Query: 123 PPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGEKIVFP 182
           PPPERRFNCLIPPP GYKVPIKWPKSRDEVWK NIPHTHLAHEKSDQNWMVVKG+KI FP
Sbjct: 122 PPPERRFNCLIPPPNGYKVPIKWPKSRDEVWKVNIPHTHLAHEKSDQNWMVVKGDKINFP 181

Query: 183 GGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSNIIAMS 242
           GGGTHFHYGADKYIAS+ANMLN+ N+ LNN GRLRTV DVGCGVASFGGYLLSS+I+ MS
Sbjct: 182 GGGTHFHYGADKYIASMANMLNYPNNVLNNGGRLRTVFDVGCGVASFGGYLLSSDILTMS 241

Query: 243 LAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLE 302
           LAPNDVHQNQIQFALERGIPA LGVLGTKRLPYPSRSFEL+HCSRCRIDWLQRDGILLLE
Sbjct: 242 LAPNDVHQNQIQFALERGIPASLGVLGTKRLPYPSRSFELSHCSRCRIDWLQRDGILLLE 301

Query: 303 LDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQKPVTND 362
           LDR+LRPGGYFAYSSPEAYAQDEEDLRIWREMS LV RMCW+IAAKRNQTVIWQKP+TND
Sbjct: 302 LDRVLRPGGYFAYSSPEAYAQDEEDLRIWREMSALVERMCWKIAAKRNQTVIWQKPLTND 361

Query: 363 CYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLTSPPPR 422
           CYL+REPGT+PPLCRSD+DPDAVWGV MEACIT YSDHDHK KGSGLAPWPARLTSPPPR
Sbjct: 362 CYLEREPGTQPPLCRSDNDPDAVWGVNMEACITSYSDHDHKTKGSGLAPWPARLTSPPPR 421

Query: 423 LQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAALKDKDV 482
           L DFGYST MFEKDTE+WR+RV++YW+LLSP+IESDT+RN+MDMKA+MGSF AALK+KDV
Sbjct: 422 LADFGYSTGMFEKDTELWRQRVDTYWDLLSPRIESDTVRNIMDMKASMGSFAAALKEKDV 481

Query: 483 WVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKKECGAE 542
           WVMNVVPEDGPNTLKLIYDRGL+G  ++WCEAFSTYPRTYDLLHAW + SD++KK C   
Sbjct: 482 WVMNVVPEDGPNTLKLIYDRGLMGAVHSWCEAFSTYPRTYDLLHAWDIISDIKKKGCSEV 541

Query: 543 DLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDEVVFIV 602
           DLLLEMDR+LRP+GFIIIRDKQ VVD +KKYL ALHWE V  T   SDS+ DSD VVFIV
Sbjct: 542 DLLLEMDRILRPSGFIIIRDKQRVVDFVKKYLKALHWEEVG-TKTDSDSDQDSDNVVFIV 601

Query: 603 QKKLWLTSESVRDSE 618
           QKKLWLTSES+RD E
Sbjct: 602 QKKLWLTSESLRDME 608

BLAST of Lag0016535 vs. TAIR 10
Match: AT3G23300.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 1029.6 bits (2661), Expect = 1.0e-300
Identity = 487/620 (78.55%), Postives = 539/620 (86.94%), Query Frame = 0

Query: 3   RGRSDGTQKKRLITTICVVAIFLGFLYAYYGSIFGNQDRGASALQHGSKSLSHYLMGSED 62
           RGRS+G +KK +I  +CV ++ L F+Y ++GS          A+++G K     L G +D
Sbjct: 2   RGRSEGGKKKPVIVLLCVASVVLVFVYLFFGS------SNHKAIEYGRKL---GLGGDDD 61

Query: 63  SEESSTSTEQEDPEDDVV-----PKSYPVCDDRHSELIPCLDRHLIYQMRLKLDLSLMEH 122
                  T      +DVV     P+S+PVCDDRHSELIPCLDR+LIYQMRLKLDLSLMEH
Sbjct: 62  DSTKKDDTSSSFYVEDVVGNGFTPRSFPVCDDRHSELIPCLDRNLIYQMRLKLDLSLMEH 121

Query: 123 YERHCPPPERRFNCLIPPPAGYKVPIKWPKSRDEVWKANIPHTHLAHEKSDQNWMVVKGE 182
           YERHCPPPERRFNCLIPPP GYK+PIKWPKSRDEVWK NIPHTHLAHEKSDQNWMVVKGE
Sbjct: 122 YERHCPPPERRFNCLIPPPPGYKIPIKWPKSRDEVWKVNIPHTHLAHEKSDQNWMVVKGE 181

Query: 183 KIVFPGGGTHFHYGADKYIASIANMLNFSNDNLNNQGRLRTVLDVGCGVASFGGYLLSSN 242
           KI FPGGGTHFHYGADKYIAS+ANMLNF N+ LNN GRLRT LDVGCGVASFGGYLL+S 
Sbjct: 182 KINFPGGGTHFHYGADKYIASMANMLNFPNNVLNNGGRLRTFLDVGCGVASFGGYLLASE 241

Query: 243 IIAMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDG 302
           I+ MSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDG
Sbjct: 242 IMTMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDG 301

Query: 303 ILLLELDRLLRPGGYFAYSSPEAYAQDEEDLRIWREMSDLVSRMCWRIAAKRNQTVIWQK 362
           ILLLELDR+LRPGGYFAYSSPEAYAQDEEDLRIWREMS LV RMCW IAAKRNQTVIWQK
Sbjct: 302 ILLLELDRVLRPGGYFAYSSPEAYAQDEEDLRIWREMSALVGRMCWTIAAKRNQTVIWQK 361

Query: 363 PVTNDCYLQREPGTRPPLCRSDDDPDAVWGVQMEACITPYSDHDHKAKGSGLAPWPARLT 422
           P+TNDCYL REPGT+PPLC SD DPDAV+GV MEACIT YSDHDHK KGSGLAPWPARLT
Sbjct: 362 PLTNDCYLGREPGTQPPLCNSDSDPDAVYGVNMEACITQYSDHDHKTKGSGLAPWPARLT 421

Query: 423 SPPPRLQDFGYSTEMFEKDTEIWRRRVESYWNLLSPKIESDTIRNVMDMKANMGSFGAAL 482
           SPPPRL DFGYST++FEKDTE WR+RV++YW+LLSPKI+SDT+RN+MDMKA+MGSF AAL
Sbjct: 422 SPPPRLADFGYSTDIFEKDTETWRQRVDTYWDLLSPKIQSDTVRNIMDMKASMGSFAAAL 481

Query: 483 KDKDVWVMNVVPEDGPNTLKLIYDRGLIGTTNNWCEAFSTYPRTYDLLHAWTVFSDVEKK 542
           K+KDVWVMNVVPEDGPNTLKLIYDRGL+G  ++WCEAFSTYPRTYDLLHAW + SD++K+
Sbjct: 482 KEKDVWVMNVVPEDGPNTLKLIYDRGLMGAVHSWCEAFSTYPRTYDLLHAWDIISDIKKR 541

Query: 543 ECGAEDLLLEMDRMLRPTGFIIIRDKQSVVDLIKKYLPALHWETVATTDASSDSELDSDE 602
            C AEDLLLEMDR+LRP+GFI+IRDKQSVVDL+KKYL ALHWE V T  A S+S+ DSD 
Sbjct: 542 GCSAEDLLLEMDRILRPSGFILIRDKQSVVDLVKKYLKALHWEAVETKTA-SESDQDSDN 601

Query: 603 VVFIVQKKLWLTSESVRDSE 618
           V+ IVQKKLWLTSES+RD E
Sbjct: 602 VILIVQKKLWLTSESLRDLE 611

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460648.10.0e+0096.43PREDICTED: probable methyltransferase PMT3 [Cucumis melo] >KAA0065644.1 putative... [more]
XP_023548079.10.0e+0095.62probable methyltransferase PMT3 [Cucurbita pepo subsp. pepo] >XP_023548080.1 pro... [more]
XP_022958907.10.0e+0095.62probable methyltransferase PMT3 [Cucurbita moschata] >XP_022958908.1 probable me... [more]
XP_038874564.10.0e+0095.62probable methyltransferase PMT3 [Benincasa hispida] >XP_038874565.1 probable met... [more]
XP_023006143.10.0e+0095.79probable methyltransferase PMT3 [Cucurbita maxima] >XP_023006144.1 probable meth... [more]
Match NameE-valueIdentityDescription
Q940J91.3e-30579.11Probable methyltransferase PMT8 OS=Arabidopsis thaliana OX=3702 GN=At1g04430 PE=... [more]
Q93YV71.7e-30580.00Probable methyltransferase PMT3 OS=Arabidopsis thaliana OX=3702 GN=At4g14360 PE=... [more]
Q8H1181.4e-29978.55Probable methyltransferase PMT1 OS=Arabidopsis thaliana OX=3702 GN=At3g23300 PE=... [more]
Q8VZV76.1e-23463.99Probable methyltransferase PMT9 OS=Arabidopsis thaliana OX=3702 GN=At5g14430 PE=... [more]
Q94KE11.1e-14547.41Probable methyltransferase PMT10 OS=Arabidopsis thaliana OX=3702 GN=At1g77260 PE... [more]
Match NameE-valueIdentityDescription
A0A5D3BQ040.0e+0096.43Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold772G00... [more]
A0A1S3CDD70.0e+0096.43Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499424 PE=3 SV=1[more]
A0A6J1H3460.0e+0095.62Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460059 PE=3 SV=1[more]
A0A6J1KZD40.0e+0095.79Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111498974 PE=3 SV=1[more]
A0A0A0LI730.0e+0095.62Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G174120 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G04430.19.4e-30779.11S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G04430.29.4e-30779.11S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G14360.11.2e-30680.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G14360.21.2e-30680.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G23300.11.0e-30078.55S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 208..357
e-value: 9.1E-11
score: 43.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..83
NoneNo IPR availablePANTHERPTHR10108:SF1082S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE-RELATEDcoord: 1..614
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 93..604
e-value: 1.3E-231
score: 769.5
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePANTHERPTHR10108SAM-DEPENDENT METHYLTRANSFERASEcoord: 1..614
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 431..578
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 119..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016535.1Lag0016535.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity