Lag0016459 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0016459
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	S-protein homolog
Location	chr12: 37982681 .. 37983115 (+)
RNA-Seq Expression	Lag0016459
Synteny	Lag0016459

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAAAAAATTCTCAACACCATCATCTTTCTCTTCTTCCTCTCAGCATTCTCCTTCTTCATCTTCATCGTACAACCAGACAGGTTGTACCTACACGGCGTCGTTTTCGACGTTCGAGTCATCAACGGCTTCAAAAACAACTCATCTCTGCCTTTGGTGATATGGTGTTCCTCAAAAGACAGCGATTTGGGGGGCAGAGCCTTGCAAGAGCACGATGATTTCAGCTGGAGAGTGACAACAAATCTGTGGAAGAGCAACCACTTCTCTTGTACCATGAAATGGGATGCCAAAAGAAGGTCCTTCGATGCGTTTAAGGTTCCTAGAGACTTCTACCGATGTGGGAATTTCAAGAAGTGTTCTTGGTTGGTTAGGGAAGATGGCTTCTATTTTAGCCCTGATGAGGTTAATTGGAAGAGGGATTTTCAGTGGTGA

mRNA sequence

ATGAAGAAAAAAATTCTCAACACCATCATCTTTCTCTTCTTCCTCTCAGCATTCTCCTTCTTCATCTTCATCGTACAACCAGACAGGTTGTACCTACACGGCGTCGTTTTCGACGTTCGAGTCATCAACGGCTTCAAAAACAACTCATCTCTGCCTTTGGTGATATGGTGTTCCTCAAAAGACAGCGATTTGGGGGGCAGAGCCTTGCAAGAGCACGATGATTTCAGCTGGAGAGTGACAACAAATCTGTGGAAGAGCAACCACTTCTCTTGTACCATGAAATGGGATGCCAAAAGAAGGTCCTTCGATGCGTTTAAGGTTCCTAGAGACTTCTACCGATGTGGGAATTTCAAGAAGTGTTCTTGGTTGGTTAGGGAAGATGGCTTCTATTTTAGCCCTGATGAGGTTAATTGGAAGAGGGATTTTCAGTGGTGA

Coding sequence (CDS)

ATGAAGAAAAAAATTCTCAACACCATCATCTTTCTCTTCTTCCTCTCAGCATTCTCCTTCTTCATCTTCATCGTACAACCAGACAGGTTGTACCTACACGGCGTCGTTTTCGACGTTCGAGTCATCAACGGCTTCAAAAACAACTCATCTCTGCCTTTGGTGATATGGTGTTCCTCAAAAGACAGCGATTTGGGGGGCAGAGCCTTGCAAGAGCACGATGATTTCAGCTGGAGAGTGACAACAAATCTGTGGAAGAGCAACCACTTCTCTTGTACCATGAAATGGGATGCCAAAAGAAGGTCCTTCGATGCGTTTAAGGTTCCTAGAGACTTCTACCGATGTGGGAATTTCAAGAAGTGTTCTTGGTTGGTTAGGGAAGATGGCTTCTATTTTAGCCCTGATGAGGTTAATTGGAAGAGGGATTTTCAGTGGTGA

Protein sequence

MKKKILNTIIFLFFLSAFSFFIFIVQPDRLYLHGVVFDVRVINGFKNNSSLPLVIWCSSKDSDLGGRALQEHDDFSWRVTTNLWKSNHFSCTMKWDAKRRSFDAFKVPRDFYRCGNFKKCSWLVREDGFYFSPDEVNWKRDFQW

Homology

[Close]

BLAST of Lag0016459 vs. NCBI nr
Match: XP_023000578.1 (S-protein homolog 5 [Cucurbita maxima])

HSP 1 Score: 282.7 bits (722), Expect = 1.7e-72
Identity = 128/144 (88.89%), Postives = 136/144 (94.44%), Query Frame = 0


Query: 1   MKKKILNTIIFLFFLSAFSFFIFIVQPDRLYLHGVVFDVRVINGFKNNSSLPLVIWCSSK 60
           MKKKILNTI+ L F+SAFSFFIFI+QPDRLYLHGVV++VRVINGF NNSSLPLVIWCSSK
Sbjct: 1   MKKKILNTILLLLFVSAFSFFIFIIQPDRLYLHGVVYEVRVINGFTNNSSLPLVIWCSSK 60

Query: 61  DSDLGGRALQEHDDFSWRVTTNLWKSNHFSCTMKWDAKRRSFDAFKVPRDFYRCGNFKKC 120
           DSDLGGRALQEHDDFSWRVTT LW +N F CTMKWDAKR+SFDAFKVPRDFYRCG FKKC
Sbjct: 61  DSDLGGRALQEHDDFSWRVTTKLWNTNQFCCTMKWDAKRKSFDAFKVPRDFYRCGIFKKC 120

Query: 121 SWLVREDGFYFSPDEVNWKRDFQW 145
           SWLVREDGFYFSPDEVNWK+DFQW
Sbjct: 121 SWLVREDGFYFSPDEVNWKKDFQW 144

[Close]

BLAST of Lag0016459 vs. NCBI nr
Match: XP_023515117.1 (S-protein homolog 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 280.8 bits (717), Expect = 6.6e-72
Identity = 126/144 (87.50%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 1   MKKKILNTIIFLFFLSAFSFFIFIVQPDRLYLHGVVFDVRVINGFKNNSSLPLVIWCSSK 60
           MKKKILN I+ L F+SAFSFFIFI+QPDRLYLHGVV++VRVINGF NNSSLPLVIWCSSK
Sbjct: 1   MKKKILNAIVLLLFISAFSFFIFIIQPDRLYLHGVVYEVRVINGFTNNSSLPLVIWCSSK 60

Query: 61  DSDLGGRALQEHDDFSWRVTTNLWKSNHFSCTMKWDAKRRSFDAFKVPRDFYRCGNFKKC 120
           DSDLGGRALQEHDDFSWRVTT +W +N F CTMKWDAKR+SFDAFKVPRDFYRCG FKKC
Sbjct: 61  DSDLGGRALQEHDDFSWRVTTKMWNTNQFCCTMKWDAKRKSFDAFKVPRDFYRCGIFKKC 120

Query: 121 SWLVREDGFYFSPDEVNWKRDFQW 145
           SWLVREDGFYFSPDEVNWK+DFQW
Sbjct: 121 SWLVREDGFYFSPDEVNWKKDFQW 144

[Close]

BLAST of Lag0016459 vs. NCBI nr
Match: XP_022964484.1 (S-protein homolog 5-like [Cucurbita moschata])

HSP 1 Score: 280.8 bits (717), Expect = 6.6e-72
Identity = 127/144 (88.19%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 1   MKKKILNTIIFLFFLSAFSFFIFIVQPDRLYLHGVVFDVRVINGFKNNSSLPLVIWCSSK 60
           MKKKILN I+ L F+SAFSFFIFI+QPDRLYLHGVV++VRVINGF NNSSLPLVIWCSSK
Sbjct: 1   MKKKILNVILLLLFVSAFSFFIFIIQPDRLYLHGVVYEVRVINGFTNNSSLPLVIWCSSK 60

Query: 61  DSDLGGRALQEHDDFSWRVTTNLWKSNHFSCTMKWDAKRRSFDAFKVPRDFYRCGNFKKC 120
           DSDLGGRALQEHDDFSWRVTT LW +N F CTMKWDAKR+SFDAFKVPRDFYRCG FKKC
Sbjct: 61  DSDLGGRALQEHDDFSWRVTTKLWNTNQFCCTMKWDAKRKSFDAFKVPRDFYRCGIFKKC 120

Query: 121 SWLVREDGFYFSPDEVNWKRDFQW 145
           SWLVREDGFYFSPDEVNWK+DFQW
Sbjct: 121 SWLVREDGFYFSPDEVNWKKDFQW 144

[Close]

BLAST of Lag0016459 vs. NCBI nr
Match: XP_016902587.1 (PREDICTED: uncharacterized protein LOC103499490 [Cucumis melo] >KAA0032040.1 self-incompatibility family protein [Cucumis melo var. makuwa] >TYK16860.1 self-incompatibility family protein [Cucumis melo var. makuwa])

HSP 1 Score: 280.0 bits (715), Expect = 1.1e-71
Identity = 125/143 (87.41%), Postives = 134/143 (93.71%), Query Frame = 0


Query: 2   KKKILNTIIFLFFLSAFSFFIFIVQPDRLYLHGVVFDVRVINGFKNNSSLPLVIWCSSKD 61
           KKK L TI+ LF  SAFSF +FIVQPDRLYLHGV+F+VRVINGFKNNSSLPLVIWCSSK+
Sbjct: 3   KKKFLGTILLLFSFSAFSFIVFIVQPDRLYLHGVIFEVRVINGFKNNSSLPLVIWCSSKE 62

Query: 62  SDLGGRALQEHDDFSWRVTTNLWKSNHFSCTMKWDAKRRSFDAFKVPRDFYRCGNFKKCS 121
           SDLGGRALQEHDDFSW+VTT LWKSN FSCTMKWDAKR+SFDAFKVPRDFYRCGNFKKCS
Sbjct: 63  SDLGGRALQEHDDFSWKVTTKLWKSNQFSCTMKWDAKRKSFDAFKVPRDFYRCGNFKKCS 122

Query: 122 WLVREDGFYFSPDEVNWKRDFQW 145
           WLV EDGFYFSPDE+NWK+DFQW
Sbjct: 123 WLVTEDGFYFSPDEINWKKDFQW 145

[Close]

BLAST of Lag0016459 vs. NCBI nr
Match: XP_038875976.1 (S-protein homolog 5-like isoform X1 [Benincasa hispida])

HSP 1 Score: 279.3 bits (713), Expect = 1.9e-71
Identity = 125/143 (87.41%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 2   KKKILNTIIFLFFLSAFSFFIFIVQPDRLYLHGVVFDVRVINGFKNNSSLPLVIWCSSKD 61
           KKKIL+TI+ LFF S+FSFF+FI+ PDR YLH VVF+VRVINGF+NNSSLPLVIWCSSK+
Sbjct: 16  KKKILSTILVLFFFSSFSFFMFIIHPDRFYLHDVVFEVRVINGFRNNSSLPLVIWCSSKE 75

Query: 62  SDLGGRALQEHDDFSWRVTTNLWKSNHFSCTMKWDAKRRSFDAFKVPRDFYRCGNFKKCS 121
           SDLGGRALQEHDDFSW+VTT LWKSN FSCTMKWDAKRRSFDAFKVPRDFYRCGNFKKCS
Sbjct: 76  SDLGGRALQEHDDFSWKVTTKLWKSNQFSCTMKWDAKRRSFDAFKVPRDFYRCGNFKKCS 135

Query: 122 WLVREDGFYFSPDEVNWKRDFQW 145
           WLV EDGFYFSPDEVNWK+DFQW
Sbjct: 136 WLVTEDGFYFSPDEVNWKKDFQW 158

[Close]

BLAST of Lag0016459 vs. ExPASy Swiss-Prot
Match: F4JLQ5 (S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 8.6e-06
Identity = 26/83 (31.33%), Postives = 41/83 (49.40%), Query Frame = 0


Query: 49  SSLPLVIWCSSKDSDLGGRALQEHDDFSWRVTTNLWKSNHFSCTMKWDAKRRSFDAFKVP 108
           + L L+  C SKD DLG R LQ  + +S+      +    + C+  W  +  SFD +K  
Sbjct: 58  NQLTLLYHCKSKDDDLGNRTLQPGESWSFSFGRQFFGRTLYFCSFSWPNESHSFDIYKDH 117

Query: 109 RDF---YRCGNFKKCSWLVREDG 129
           RD     +C +  +C W +R +G
Sbjct: 118 RDSGGDNKCES-DRCVWKIRRNG 139

The following BLAST results are available for this feature:

BLAST of Lag0016459 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000578.1	1.7e-72	88.89	S-protein homolog 5 [Cucurbita maxima]	[more]
XP_023515117.1	6.6e-72	87.50	S-protein homolog 5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022964484.1	6.6e-72	88.19	S-protein homolog 5-like [Cucurbita moschata]	[more]
XP_016902587.1	1.1e-71	87.41	PREDICTED: uncharacterized protein LOC103499490 [Cucumis melo] >KAA0032040.1 sel...	[more]
XP_038875976.1	1.9e-71	87.41	S-protein homolog 5-like isoform X1 [Benincasa hispida]	[more]

BLAST of Lag0016459 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JLQ5	8.6e-06	31.33	S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010264	Plant self-incompatibility S1	PFAM	PF05938	Self-incomp_S1	coord: 39..144 e-value: 3.3E-24 score: 85.4
IPR010264	Plant self-incompatibility S1	PANTHER	PTHR31232	FAMILY NOT NAMED	coord: 9..144
None	No IPR available	PANTHER	PTHR31232:SF8	BNAA01G23250D PROTEIN	coord: 9..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0016459.1	Lag0016459.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0060320	rejection of self pollen
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane