Lag0016426 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016426
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPectinesterase
Locationchr12: 37635080 .. 37637000 (-)
RNA-Seq ExpressionLag0016426
SyntenyLag0016426
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGATGATAATATTTGTTAGAGCAACAGAAGGTCAGGCTTATAGAACTGTGGGAGCGAAGAAATTGCCATGGCGGACTCTGGTCGTCGACCAGAGCGGCCATGGAAATTTCTCCAACATTCAAGCCGCCATTGATTCTGTTCCTTCAAATAATAAGTTTTGGGTCTCCATCTTCGTCAACGCTGGCCTTTACAGGTAATTCAATTTCTTCTCTTTTCCTTTTTTCTTTTATCTTCGATGTTCGTCACACGTAGACGTTGCTAGAGGAGACTGTCACAATGTTACTGATGGTTTTGAGATGAGAAATTATAAAAGTATTTTACGTCGGTAACTATTGATTATCCATACGCATGCACAATGTTAAGTATCTTTCTCGATCACAAATTTATAAATTTTCGTTGCAATAATATGCGACGAATAGATATTTCGTCGCAAATGTGGGACAAACCCAAGTTTTCATCGCTCACTGTTCGACGATTCTATTTTCGTCGCTAGAAGGTCTATAGGATGAGATTCAAATATGAGACTTTTTGGTTGAGGATATATGTTTTAACCAATTGAACTACGTTTGTTTAATTTTAGAGAGAATAAAATGAGTTTTTTTCCATTGTTTTGAAGAATATAAAATAATTGGATTTTTTCATGTAAAATTTGAAACCAAATATTATTAGGTTTGAATTTGGTTGATTTTGATAGTTTGGTAGGTTTTGGTTTTTGTTAGGGCAACTAGGTTTGATTTTTTGAGGGGTATATTTTTGGTAAGAATTTTTATTTATTTATTTATTTATTTAATGGGTTTGATTAAAGTTTCAATATGGTAGAAAACTGGTTTGATTTTGTAATTAAGGAGTCAATTTTGAGCATTATTATTATTTTTAATTGATAGGTCTAGGTACCAATTTTAGCCAAAAATGGATACAAATTAACTAGTTTATTCCTTAATTATTTCCCCCCTAATTTAATATTTTCATCTCTCTCTCTCTCTAGATTATGATTTTCGATTATTAACTTAAATAATTCACCTCTATTATTTTCAATTACTTTGAAATTTAAATTTTATATTCTTAAGAAAATCATTCTAATACATTTTCACTTCGATTTAAAACCTTATTGATTTCTTAAATAATAAAGTTTCCATGTAGGGTAAAATAATGTAATATTGGGAATAATTTATATAACAAAATTTTGAATGAAAATGATGAAAATTATTGGTTGAGGAATTTAAAAAGTGATTTTTTTTTTTAATTTTAATTTTAGAGAGAAGGTGAAAATTCCATATGATAAGCCATATATAATTCTGAAAGGACAAGGAAAAAGAAGAACAAAGGTTGTCTGGGACGACCATCTCACTGTAGCTCAAAGCCCAACATTCACTTCTTCAGCTGATAATATTGTCGTCAAATCTATTACTTTTGTGGTAAGTAAATATATATATATATTGAAATTTACATTTATATTCAAATAAAAGTTGTTGAATATTAGGGTTTGATTTTGTTTTATTAATTTTTTTTTTCTTAATTTTCAAATTAGGTTGTTTGATTAACCCAATTAGACTAACATATAAAAAAAAAAAAAAAAAAGAGATTGTGAATAATATTTGAATTGAATTGTTTGGGTATTAGAATTCGTATAATTATCCATGGAAGAATGGAAATCCAAGGGTTCCAGCAGTGGCAGCAATGATCACAGGCGACAAGTCGTCTTTTTACAAATGTGGTTTCTATGGAGTGCAAGACACATTGTGGGATGACCAAGGACGACATTACTATCATCGTTGTACAATCCAAGGAGCTGTCGACTTTATTTTTGGAACTGCCCAATCCATTTTTCAGGTACGTTTTTCTTTTTTGGGTAAAATACATTTTTGGTCCCTCATGTTTCGAGTTGGTTTCCAATAGGTCCCTAGGTTTCAAAATTGA

mRNA sequence

ATGACGATGATAATATTTGTTAGAGCAACAGAAGGTCAGGCTTATAGAACTGTGGGAGCGAAGAAATTGCCATGGCGGACTCTGGTCGTCGACCAGAGCGGCCATGGAAATTTCTCCAACATTCAAGCCGCCATTGATTCTGTTCCTTCAAATAATAAGTTTTGGGTCTCCATCTTCGTCAACGCTGGCCTTTACAGAGAGAAGGTGAAAATTCCATATGATAAGCCATATATAATTCTGAAAGGACAAGGAAAAAGAAGAACAAAGGTTGTCTGGGACGACCATCTCACTGTAGCTCAAAGCCCAACATTCACTTCTTCAGCTGATAATATTGTCGTCAAATCTATTACTTTTGTGAATTCGTATAATTATCCATGGAAGAATGGAAATCCAAGGGTTCCAGCAGTGGCAGCAATGATCACAGGCGACAAGTCGTCTTTTTACAAATGTGGTTTCTATGGAGTGCAAGACACATTGTGGGATGACCAAGGACGACATTACTATCATCGTTGTACAATCCAAGGAGCTGTCGACTTTATTTTTGGAACTGCCCAATCCATTTTTCAGGTCCCTAGGTTTCAAAATTGA

Coding sequence (CDS)

ATGACGATGATAATATTTGTTAGAGCAACAGAAGGTCAGGCTTATAGAACTGTGGGAGCGAAGAAATTGCCATGGCGGACTCTGGTCGTCGACCAGAGCGGCCATGGAAATTTCTCCAACATTCAAGCCGCCATTGATTCTGTTCCTTCAAATAATAAGTTTTGGGTCTCCATCTTCGTCAACGCTGGCCTTTACAGAGAGAAGGTGAAAATTCCATATGATAAGCCATATATAATTCTGAAAGGACAAGGAAAAAGAAGAACAAAGGTTGTCTGGGACGACCATCTCACTGTAGCTCAAAGCCCAACATTCACTTCTTCAGCTGATAATATTGTCGTCAAATCTATTACTTTTGTGAATTCGTATAATTATCCATGGAAGAATGGAAATCCAAGGGTTCCAGCAGTGGCAGCAATGATCACAGGCGACAAGTCGTCTTTTTACAAATGTGGTTTCTATGGAGTGCAAGACACATTGTGGGATGACCAAGGACGACATTACTATCATCGTTGTACAATCCAAGGAGCTGTCGACTTTATTTTTGGAACTGCCCAATCCATTTTTCAGGTCCCTAGGTTTCAAAATTGA

Protein sequence

MTMIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFVNAGLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFIFGTAQSIFQVPRFQN
Homology
BLAST of Lag0016426 vs. NCBI nr
Match: XP_038875009.1 (probable pectinesterase 29 [Benincasa hispida])

HSP 1 Score: 361.3 bits (926), Expect = 5.2e-96
Identity = 167/189 (88.36%), Postives = 176/189 (93.12%), Query Frame = 0

Query: 1   MTMIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFV 60
           + MIIFVR  EGQ+YR+VG KKL W+TL+VDQ+GHGNFS IQAAIDS+PSNN+FWVSI V
Sbjct: 13  LMMIIFVRGNEGQSYRSVGMKKLTWKTLIVDQNGHGNFSTIQAAIDSIPSNNRFWVSIHV 72

Query: 61  NAGLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN 120
             G+YREKVKIPYDKPYIILKGQ KR TKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN
Sbjct: 73  KPGVYREKVKIPYDKPYIILKGQSKRTTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN 132

Query: 121 SYNYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI 180
           SYNYPW NGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI
Sbjct: 133 SYNYPWNNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI 192

Query: 181 FGTAQSIFQ 190
           FG AQSIFQ
Sbjct: 193 FGAAQSIFQ 201

BLAST of Lag0016426 vs. NCBI nr
Match: KAE8651766.1 (hypothetical protein Csa_006597 [Cucumis sativus])

HSP 1 Score: 352.8 bits (904), Expect = 1.9e-93
Identity = 162/189 (85.71%), Postives = 174/189 (92.06%), Query Frame = 0

Query: 1   MTMIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFV 60
           + M+IFVR  EG+ YR VG+KK  W+TL+VD+ GHGNFS IQAAIDSVPSNN+FWVSI +
Sbjct: 12  LMMMIFVRGNEGEPYRRVGSKKFAWKTLIVDKKGHGNFSTIQAAIDSVPSNNRFWVSIHI 71

Query: 61  NAGLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN 120
             GLYREKVKIPYDKPYIILKG  KRRTKVVWDDHLTVAQSPTFTSSADNIVVKSI+FVN
Sbjct: 72  RPGLYREKVKIPYDKPYIILKGHRKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVN 131

Query: 121 SYNYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI 180
           SYNYPWKNGNPRVPAVAAMITGDKSSFY+CGFYGVQDTLWD+QGRHYYHRCTIQGAVDFI
Sbjct: 132 SYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFI 191

Query: 181 FGTAQSIFQ 190
           FG AQSIFQ
Sbjct: 192 FGAAQSIFQ 200

BLAST of Lag0016426 vs. NCBI nr
Match: XP_011650140.1 (probable pectinesterase 29 [Cucumis sativus])

HSP 1 Score: 352.8 bits (904), Expect = 1.9e-93
Identity = 162/189 (85.71%), Postives = 174/189 (92.06%), Query Frame = 0

Query: 1   MTMIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFV 60
           + M+IFVR  EG+ YR VG+KK  W+TL+VD+ GHGNFS IQAAIDSVPSNN+FWVSI +
Sbjct: 12  LMMMIFVRGNEGEPYRRVGSKKFAWKTLIVDKKGHGNFSTIQAAIDSVPSNNRFWVSIHI 71

Query: 61  NAGLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN 120
             GLYREKVKIPYDKPYIILKG  KRRTKVVWDDHLTVAQSPTFTSSADNIVVKSI+FVN
Sbjct: 72  RPGLYREKVKIPYDKPYIILKGHRKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVN 131

Query: 121 SYNYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI 180
           SYNYPWKNGNPRVPAVAAMITGDKSSFY+CGFYGVQDTLWD+QGRHYYHRCTIQGAVDFI
Sbjct: 132 SYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFI 191

Query: 181 FGTAQSIFQ 190
           FG AQSIFQ
Sbjct: 192 FGAAQSIFQ 200

BLAST of Lag0016426 vs. NCBI nr
Match: KAA0033293.1 (putative pectinesterase 29 [Cucumis melo var. makuwa] >TYK14815.1 putative pectinesterase 29 [Cucumis melo var. makuwa])

HSP 1 Score: 350.1 bits (897), Expect = 1.2e-92
Identity = 160/189 (84.66%), Postives = 174/189 (92.06%), Query Frame = 0

Query: 1   MTMIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFV 60
           + MIIFVR  EG++YR +G KK  W+TL+VD++GHGNFS IQAAIDSVPS+N+FWVSI +
Sbjct: 11  LMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHI 70

Query: 61  NAGLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN 120
             GLYREKVKIPYDKPYIILKG  KR TKVVWDDHLTVAQSPTFTSSADNIVVKSI+FVN
Sbjct: 71  RPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVN 130

Query: 121 SYNYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI 180
           SYNYPWKNGNPRVPAVAAMITGDKSSFY+CGFYGVQDTLWD+QGRHYYHRCTIQGAVDFI
Sbjct: 131 SYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFI 190

Query: 181 FGTAQSIFQ 190
           FG AQSIFQ
Sbjct: 191 FGAAQSIFQ 199

BLAST of Lag0016426 vs. NCBI nr
Match: XP_008457276.1 (PREDICTED: probable pectinesterase 29 [Cucumis melo])

HSP 1 Score: 350.1 bits (897), Expect = 1.2e-92
Identity = 160/189 (84.66%), Postives = 174/189 (92.06%), Query Frame = 0

Query: 1   MTMIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFV 60
           + MIIFVR  EG++YR +G KK  W+TL+VD++GHGNFS IQAAIDSVPS+N+FWVSI +
Sbjct: 11  LMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHI 70

Query: 61  NAGLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN 120
             GLYREKVKIPYDKPYIILKG  KR TKVVWDDHLTVAQSPTFTSSADNIVVKSI+FVN
Sbjct: 71  RPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVN 130

Query: 121 SYNYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI 180
           SYNYPWKNGNPRVPAVAAMITGDKSSFY+CGFYGVQDTLWD+QGRHYYHRCTIQGAVDFI
Sbjct: 131 SYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFI 190

Query: 181 FGTAQSIFQ 190
           FG AQSIFQ
Sbjct: 191 FGAAQSIFQ 199

BLAST of Lag0016426 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 7.7e-66
Identity = 118/163 (72.39%), Postives = 134/163 (82.21%), Query Frame = 0

Query: 30  VDQSGHGNFSNIQAAIDSVPSNNKFWVSIFVNAGLYREKVKIPYDKPYIILKGQGKRRTK 89
           VDQSGHGNF+ IQ AIDSVP NN+ W  I V AGLYREK+KIPY+KP+I+L G GKR T+
Sbjct: 36  VDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRLTR 95

Query: 90  VVWDDHLTVAQSPTFTSSADNIVVKSITFVNSYNYPWK---NGNPRVPAVAAMITGDKSS 149
           V WDDH +VAQSPTF++ ADN VVKSITF NSYN+P K   N NPR PAVAA+I GDKS+
Sbjct: 96  VEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDKSA 155

Query: 150 FYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFIFGTAQSIFQ 190
           FY  GF G+QDTLWD  GRHY+HRCTIQGAVDFIFGT QSI+Q
Sbjct: 156 FYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQ 198

BLAST of Lag0016426 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 6.7e-62
Identity = 111/165 (67.27%), Postives = 128/165 (77.58%), Query Frame = 0

Query: 28  LVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFVNAGLYREKVKIPYDKPYIILKGQGKRR 87
           ++VDQSGHGNF+ IQ AIDSVP NN  W  I V AGLYREK+ IP  KP+I++ G GKR 
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 88  TKVVWDDHLTVAQSPTFTSSADNIVVKSITFVNSYNYPWK---NGNPRVPAVAAMITGDK 147
           T+V WDDH ++AQSPTF + ADN VVK ITF NSYN+P     N NPRVPAVAA I GDK
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGGDK 148

Query: 148 SSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFIFGTAQSIFQ 190
           S+FY  GF G+QDTLWD  GRHY+HRCTIQGAVDFI G+ QSI+Q
Sbjct: 149 SAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQ 193

BLAST of Lag0016426 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 1.5e-40
Identity = 79/163 (48.47%), Postives = 107/163 (65.64%), Query Frame = 0

Query: 27  TLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFVNAGLYREKVKIPYDKPYIILKGQGKR 86
           T+ VD +G GNF+ +Q+AIDS+   N  W+ +F   G+YREKV IP +K +I L+G+G  
Sbjct: 34  TITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQGKGIE 93

Query: 87  RTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVNSYNYPWKNGNPRVPAVAAMITGDKSS 146
           +T + +DDH     S TFT+ AD+IV+  ITF N+YN    N    VPAVAA + GD+  
Sbjct: 94  QTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPNNKREIVPAVAARMLGDRYV 153

Query: 147 FYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFIFGTAQSIFQ 190
                F G+QDTL+D +GRHYY RC I G +DFIFG  QS+F+
Sbjct: 154 VTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFK 196

BLAST of Lag0016426 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.0e-39
Identity = 77/164 (46.95%), Postives = 104/164 (63.41%), Query Frame = 0

Query: 26  RTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFVNAGLYREKVKIPYDKPYIILKGQGK 85
           +T++V+ +    F  +Q+AIDS+P  N+ W+ I ++ G+Y EKV IP  K YI ++G G 
Sbjct: 39  KTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGGGI 98

Query: 86  RRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVNSYNYPWKNGNPRVPAVAAMITGDKS 145
            +T + + DH     S TFTS   NI++  ITF N YN    + +P  PAVAAM+ GDK 
Sbjct: 99  EKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA-SSSSPTKPAVAAMMLGDKY 158

Query: 146 SFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFIFGTAQSIFQ 190
           +     F G QDTL+DD GRHYY RC I G +DFIFG AQSIF+
Sbjct: 159 AIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFE 201

BLAST of Lag0016426 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 7.7e-34
Identity = 74/169 (43.79%), Postives = 106/169 (62.72%), Query Frame = 0

Query: 28  LVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFVNAGLYREKVKIPYDKPYIILKGQGKRR 87
           L VD  G GNFSN+Q+AID VP  +     I VN+G YREKV +  +K  ++++G+G + 
Sbjct: 91  LTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQN 150

Query: 88  TKVVWDDHLTVAQSPTFTSSADNIVVKSITFVNSYNYPWKNGNPR-------VPAVAAMI 147
           T + W+D    A+S   T+ + + VV +  F  +YN  +KN  P          AVA  I
Sbjct: 151 TSIEWND---TAKSAGNTADSFSFVVFAANF-TAYNISFKNNAPEPDPGEADAQAVALRI 210

Query: 148 TGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFIFGTAQSIFQ 190
            GD+++FY CGFYG QDTL DD+GRH++  C IQG++DFIFG  +S++Q
Sbjct: 211 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQ 255

BLAST of Lag0016426 vs. ExPASy TrEMBL
Match: A0A0A0LI69 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G138720 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.1e-94
Identity = 163/190 (85.79%), Postives = 175/190 (92.11%), Query Frame = 0

Query: 1   MTMIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFV 60
           + M+IFVR  EG+ YR VG+KK  W+TL+VD+ GHGNFS IQAAIDSVPSNN+FWVSI +
Sbjct: 12  LMMMIFVRGNEGEPYRRVGSKKFAWKTLIVDKKGHGNFSTIQAAIDSVPSNNRFWVSIHI 71

Query: 61  NAGLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN 120
             GLYREKVKIPYDKPYIILKG  KRRTKVVWDDHLTVAQSPTFTSSADNIVVKSI+FVN
Sbjct: 72  RPGLYREKVKIPYDKPYIILKGHRKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVN 131

Query: 121 SYNYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI 180
           SYNYPWKNGNPRVPAVAAMITGDKSSFY+CGFYGVQDTLWD+QGRHYYHRCTIQGAVDFI
Sbjct: 132 SYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFI 191

Query: 181 FGTAQSIFQV 191
           FG AQSIFQV
Sbjct: 192 FGAAQSIFQV 201

BLAST of Lag0016426 vs. ExPASy TrEMBL
Match: A0A5D3CTI5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00040 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 5.8e-93
Identity = 160/189 (84.66%), Postives = 174/189 (92.06%), Query Frame = 0

Query: 1   MTMIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFV 60
           + MIIFVR  EG++YR +G KK  W+TL+VD++GHGNFS IQAAIDSVPS+N+FWVSI +
Sbjct: 11  LMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHI 70

Query: 61  NAGLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN 120
             GLYREKVKIPYDKPYIILKG  KR TKVVWDDHLTVAQSPTFTSSADNIVVKSI+FVN
Sbjct: 71  RPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVN 130

Query: 121 SYNYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI 180
           SYNYPWKNGNPRVPAVAAMITGDKSSFY+CGFYGVQDTLWD+QGRHYYHRCTIQGAVDFI
Sbjct: 131 SYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFI 190

Query: 181 FGTAQSIFQ 190
           FG AQSIFQ
Sbjct: 191 FGAAQSIFQ 199

BLAST of Lag0016426 vs. ExPASy TrEMBL
Match: A0A1S3C544 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497005 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 5.8e-93
Identity = 160/189 (84.66%), Postives = 174/189 (92.06%), Query Frame = 0

Query: 1   MTMIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFV 60
           + MIIFVR  EG++YR +G KK  W+TL+VD++GHGNFS IQAAIDSVPS+N+FWVSI +
Sbjct: 11  LMMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHI 70

Query: 61  NAGLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVN 120
             GLYREKVKIPYDKPYIILKG  KR TKVVWDDHLTVAQSPTFTSSADNIVVKSI+FVN
Sbjct: 71  RPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVN 130

Query: 121 SYNYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFI 180
           SYNYPWKNGNPRVPAVAAMITGDKSSFY+CGFYGVQDTLWD+QGRHYYHRCTIQGAVDFI
Sbjct: 131 SYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFI 190

Query: 181 FGTAQSIFQ 190
           FG AQSIFQ
Sbjct: 191 FGAAQSIFQ 199

BLAST of Lag0016426 vs. ExPASy TrEMBL
Match: A0A6J1KUQ1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111498876 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.2e-91
Identity = 158/187 (84.49%), Postives = 170/187 (90.91%), Query Frame = 0

Query: 3   MIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFVNA 62
           MIIFVR  E + YR+VG KKL WRT+VVDQSGHGNFS IQAAIDSVPSNN FWVSI +N 
Sbjct: 4   MIIFVRGNENERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWVSIHINP 63

Query: 63  GLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVNSY 122
           G+YREKVKIPYDKPYIILKGQ ++ TKVVWDDH T+AQSPTFTSSADN+VVKSITFVNSY
Sbjct: 64  GIYREKVKIPYDKPYIILKGQRRKTTKVVWDDHFTLAQSPTFTSSADNVVVKSITFVNSY 123

Query: 123 NYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFIFG 182
           N+PW NGN R PAVAAMITGDK+SFYKCGFYGVQDTLWDDQGRHYYHRC IQGAVDFIFG
Sbjct: 124 NFPWTNGNRRAPAVAAMITGDKASFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFG 183

Query: 183 TAQSIFQ 190
           +AQSIFQ
Sbjct: 184 SAQSIFQ 190

BLAST of Lag0016426 vs. ExPASy TrEMBL
Match: A0A6J1H4P1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460019 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.0e-89
Identity = 155/187 (82.89%), Postives = 169/187 (90.37%), Query Frame = 0

Query: 3   MIIFVRATEGQAYRTVGAKKLPWRTLVVDQSGHGNFSNIQAAIDSVPSNNKFWVSIFVNA 62
           MIIFVR  E + YR+VG KKL WRT+VVDQSGHGNFS IQAAIDSVPSNN FW+SI +N 
Sbjct: 4   MIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHINP 63

Query: 63  GLYREKVKIPYDKPYIILKGQGKRRTKVVWDDHLTVAQSPTFTSSADNIVVKSITFVNSY 122
           G+YREKVKIPYDKPYIILKGQ ++ TKVVW+DH T+AQSPTFTSSADN+VVKSITFVNSY
Sbjct: 64  GIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSY 123

Query: 123 NYPWKNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHYYHRCTIQGAVDFIFG 182
           N+PW NGN R PAVAAMITGDK+ FYKCGFYGVQDTLWDDQGRHYYHRC IQGAVDFIFG
Sbjct: 124 NFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFG 183

Query: 183 TAQSIFQ 190
           +AQSIFQ
Sbjct: 184 SAQSIFQ 190

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875009.15.2e-9688.36probable pectinesterase 29 [Benincasa hispida][more]
KAE8651766.11.9e-9385.71hypothetical protein Csa_006597 [Cucumis sativus][more]
XP_011650140.11.9e-9385.71probable pectinesterase 29 [Cucumis sativus][more]
KAA0033293.11.2e-9284.66putative pectinesterase 29 [Cucumis melo var. makuwa] >TYK14815.1 putative pecti... [more]
XP_008457276.11.2e-9284.66PREDICTED: probable pectinesterase 29 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q4PSN07.7e-6672.39Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1[more]
Q3E9D36.7e-6267.27Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1[more]
Q4PSQ51.5e-4048.47Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2[more]
O644798.0e-3946.95Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1[more]
Q9ZQA37.7e-3443.79Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LI693.1e-9485.79Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G138720 PE=4 SV=1[more]
A0A5D3CTI55.8e-9384.66Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00040... [more]
A0A1S3C5445.8e-9384.66Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497005 PE=4 SV=1[more]
A0A6J1KUQ13.2e-9184.49Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111498876 PE=4 SV=1[more]
A0A6J1H4P11.0e-8982.89Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460019 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 28..190
e-value: 9.3E-36
score: 123.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 5..193
e-value: 9.2E-59
score: 201.4
NoneNo IPR availablePANTHERPTHR31321:SF76PECTINESTERASE 10-RELATEDcoord: 9..189
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 9..189
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 173..182
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 25..189

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016426.1Lag0016426.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity