Lag0016343 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0016343
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	PMEI domain-containing protein
Location	chr12: 36583589 .. 36584158 (-)
RNA-Seq Expression	Lag0016343
Synteny	Lag0016343

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAATGGCCATATCTCATTTCTCTCTTTACATCCTCTCCATCTTCTTCTTCTCTAATTTCCTCATTGCTCAATCCTCTCACAACATCAACACACCTCTAATCCACAAAACTTGCAAGAAAATCGCGGTCGAAAACCCGAATATTTCCTTCAAATTTTGCTCGACGTCTCTACAATCTGCCACCAAGCGCAGCCGTCGTTGCGCCGATCTCCGCCGCCTCGGCCTCCTCTCCATCGGCTTGGTTCGGAGCAATGTGACTAGCACGCGCCACTTGATCACAAAGTTAATGAGAAACAAGAGCCTTGACCCGTTTGTGAGGTCATGTTTGAGTGATTGTTTGGAACTTTATTCGGACGCGATCCCGACGGTGAAGCGGGCCGCCAGGGACTATAGGCTGAAGCGATACGCCGATGCGAACATCGCGATCGGTACGGTGATGGACGCAGCGTCGACGTGTGAGGATGGTTTCAAGGAGAAGAAAGGGGTGCGTTCGCCATTGAAGAAGAGGGATGAAGATGCTTTTGAATTGGGAGCCATTGTTCTTGCAATTATGAATTTGCTTCATTGA

mRNA sequence

ATGCCAATGGCCATATCTCATTTCTCTCTTTACATCCTCTCCATCTTCTTCTTCTCTAATTTCCTCATTGCTCAATCCTCTCACAACATCAACACACCTCTAATCCACAAAACTTGCAAGAAAATCGCGGTCGAAAACCCGAATATTTCCTTCAAATTTTGCTCGACGTCTCTACAATCTGCCACCAAGCGCAGCCGTCGTTGCGCCGATCTCCGCCGCCTCGGCCTCCTCTCCATCGGCTTGGTTCGGAGCAATGTGACTAGCACGCGCCACTTGATCACAAAGTTAATGAGAAACAAGAGCCTTGACCCGTTTGTGAGGTCATGTTTGAGTGATTGTTTGGAACTTTATTCGGACGCGATCCCGACGGTGAAGCGGGCCGCCAGGGACTATAGGCTGAAGCGATACGCCGATGCGAACATCGCGATCGGTACGGTGATGGACGCAGCGTCGACGTGTGAGGATGGTTTCAAGGAGAAGAAAGGGGTGCGTTCGCCATTGAAGAAGAGGGATGAAGATGCTTTTGAATTGGGAGCCATTGTTCTTGCAATTATGAATTTGCTTCATTGA

Coding sequence (CDS)

ATGCCAATGGCCATATCTCATTTCTCTCTTTACATCCTCTCCATCTTCTTCTTCTCTAATTTCCTCATTGCTCAATCCTCTCACAACATCAACACACCTCTAATCCACAAAACTTGCAAGAAAATCGCGGTCGAAAACCCGAATATTTCCTTCAAATTTTGCTCGACGTCTCTACAATCTGCCACCAAGCGCAGCCGTCGTTGCGCCGATCTCCGCCGCCTCGGCCTCCTCTCCATCGGCTTGGTTCGGAGCAATGTGACTAGCACGCGCCACTTGATCACAAAGTTAATGAGAAACAAGAGCCTTGACCCGTTTGTGAGGTCATGTTTGAGTGATTGTTTGGAACTTTATTCGGACGCGATCCCGACGGTGAAGCGGGCCGCCAGGGACTATAGGCTGAAGCGATACGCCGATGCGAACATCGCGATCGGTACGGTGATGGACGCAGCGTCGACGTGTGAGGATGGTTTCAAGGAGAAGAAAGGGGTGCGTTCGCCATTGAAGAAGAGGGATGAAGATGCTTTTGAATTGGGAGCCATTGTTCTTGCAATTATGAATTTGCTTCATTGA

Protein sequence

MPMAISHFSLYILSIFFFSNFLIAQSSHNINTPLIHKTCKKIAVENPNISFKFCSTSLQSATKRSRRCADLRRLGLLSIGLVRSNVTSTRHLITKLMRNKSLDPFVRSCLSDCLELYSDAIPTVKRAARDYRLKRYADANIAIGTVMDAASTCEDGFKEKKGVRSPLKKRDEDAFELGAIVLAIMNLLH

Homology

[Close]

BLAST of Lag0016343 vs. NCBI nr
Match: KAA0039956.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK24547.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 259.6 bits (662), Expect = 2.1e-65
Identity = 139/188 (73.94%), Postives = 160/188 (85.11%), Query Frame = 0


Query: 3   MAISH-FSLYILSIFFFSNFLIAQSSHNINT-PLIHKTCKKIAVENPNISFKFCSTSLQS 62
           MAI H FS++ILSIFFFSNFLI QSS  I T  LI+KTCKKI+ E+PNISF FC TSL+ 
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 63  ATKRSRRCADLRRLGLLSIGLVRSNVTSTRHLITKLMRNKSLDPFVRSCLSDCLELYSDA 122
           AT  S RC D+R LGLLSIGL+  NVTST H ITKL++NK LDPFV+SCL DCLELYSDA
Sbjct: 61  ATNHS-RCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDA 120

Query: 123 IPTVKRAARDYRLKRYADANIAIGTVMDAASTCEDGFKEKKGVRSPLKKRDEDAFELGAI 182
           IPTVK+A RDY+ KRY D N+AIG+VMDAA+TCEDGFKE+KGV SPLKKRDE+AFELGAI
Sbjct: 121 IPTVKQAMRDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAI 180

Query: 183 VLAIMNLL 189
           VL+IM+L+
Sbjct: 181 VLSIMSLV 187

[Close]

BLAST of Lag0016343 vs. NCBI nr
Match: XP_008460048.1 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 258.5 bits (659), Expect = 4.6e-65
Identity = 138/188 (73.40%), Postives = 160/188 (85.11%), Query Frame = 0


Query: 3   MAISH-FSLYILSIFFFSNFLIAQSSHNINT-PLIHKTCKKIAVENPNISFKFCSTSLQS 62
           MAI H FS++ILSIFFFSNFLI QSS  I T  LI+KTCKKI+ E+PNISF FC TSL+ 
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 63  ATKRSRRCADLRRLGLLSIGLVRSNVTSTRHLITKLMRNKSLDPFVRSCLSDCLELYSDA 122
           AT  S RC D+R LGLLSIGL+  NVTST H ITKL++NK LDPFV+SCL DCLELYSDA
Sbjct: 61  ATNHS-RCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDA 120

Query: 123 IPTVKRAARDYRLKRYADANIAIGTVMDAASTCEDGFKEKKGVRSPLKKRDEDAFELGAI 182
           IPTVK+A +DY+ KRY D N+AIG+VMDAA+TCEDGFKE+KGV SPLKKRDE+AFELGAI
Sbjct: 121 IPTVKQAMKDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAI 180

Query: 183 VLAIMNLL 189
           VL+IM+L+
Sbjct: 181 VLSIMSLV 187

[Close]

BLAST of Lag0016343 vs. NCBI nr
Match: XP_022999945.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 253.1 bits (645), Expect = 1.9e-63
Identity = 137/187 (73.26%), Postives = 155/187 (82.89%), Query Frame = 0


Query: 3   MAISHFSLYILSIFFFSNFLIAQSSHNINTPLIHKTCKKIAVENPNISFKFCSTSLQSAT 62
           MAIS FSL+ILSIFFFSNFLI QS  NI+TPLIHKTC KIA ++PN+SF FC TSLQ AT
Sbjct: 1   MAISPFSLFILSIFFFSNFLITQSFSNIDTPLIHKTCNKIAHQDPNVSFNFCLTSLQFAT 60

Query: 63  KRSRRCADLRRLGLLSIGLVRSNVTSTRHLITKLMRNKSLDPFVRSCLSDCLELYSDAIP 122
           K S    DLRRLGLLS+GLV SNVT+T H IT+L+RNK LD  V+ CL+DCLELYSDAI 
Sbjct: 61  KHS-YYVDLRRLGLLSMGLVCSNVTNTYHFITELIRNKKLDSSVKLCLADCLELYSDAIS 120

Query: 123 TVKRAARDYRLKRYADANIAIGTVMDAASTCEDGFKEKKGVRSPLKKRDEDAFELGAIVL 182
           TVK A R+YRLKRY + N+AI  VM+AA TCE+GFKEKK V SPLKKRDED FELGAIVL
Sbjct: 121 TVKLAMRNYRLKRYKNDNVAISAVMNAAETCENGFKEKK-VNSPLKKRDEDVFELGAIVL 180

Query: 183 AIMNLLH 190
           AIM++LH
Sbjct: 181 AIMSMLH 185

[Close]

BLAST of Lag0016343 vs. NCBI nr
Match: KAG6593720.1 (hypothetical protein SDJN03_13196, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 250.8 bits (639), Expect = 9.7e-63
Identity = 135/187 (72.19%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 3   MAISHFSLYILSIFFFSNFLIAQSSHNINTPLIHKTCKKIAVENPNISFKFCSTSLQSAT 62
           MAIS FSL+ILSIFFFSNFLI +S  N +TPLIHKTC KI   +PN+SF FC TSLQ AT
Sbjct: 1   MAISPFSLFILSIFFFSNFLITRSVSNTDTPLIHKTCNKIVRLDPNVSFNFCLTSLQFAT 60

Query: 63  KRSRRCADLRRLGLLSIGLVRSNVTSTRHLITKLMRNKSLDPFVRSCLSDCLELYSDAIP 122
           K S    DLRRLGLLSIGLV SNVT+T H IT+L+RNK+LD  ++ CL+DCLELYSDAI 
Sbjct: 61  KHS-YYVDLRRLGLLSIGLVCSNVTNTYHFITELIRNKNLDSSIKLCLADCLELYSDAIS 120

Query: 123 TVKRAARDYRLKRYADANIAIGTVMDAASTCEDGFKEKKGVRSPLKKRDEDAFELGAIVL 182
           TVK A ++YRL+RY DAN+AI  VMDAA TCE+GFKEKK V SPLKKRDED FELGAIVL
Sbjct: 121 TVKLAMKNYRLRRYNDANVAISAVMDAAETCENGFKEKK-VNSPLKKRDEDVFELGAIVL 180

Query: 183 AIMNLLH 190
           AIM++LH
Sbjct: 181 AIMSMLH 185

[Close]

BLAST of Lag0016343 vs. NCBI nr
Match: XP_022964389.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 250.4 bits (638), Expect = 1.3e-62
Identity = 135/187 (72.19%), Postives = 153/187 (81.82%), Query Frame = 0


Query: 3   MAISHFSLYILSIFFFSNFLIAQSSHNINTPLIHKTCKKIAVENPNISFKFCSTSLQSAT 62
           MAIS FSL+ILSIFFFSNFLI +S  N +TPLIHKTC KI  ++PN+SF FC TSLQ A 
Sbjct: 1   MAISPFSLFILSIFFFSNFLITRSVSNTDTPLIHKTCNKIVRQDPNVSFNFCLTSLQFAI 60

Query: 63  KRSRRCADLRRLGLLSIGLVRSNVTSTRHLITKLMRNKSLDPFVRSCLSDCLELYSDAIP 122
           K S    DLRRLGLLSIGLV SNVT+T H IT+L+RNK+LD  ++ CL DCLELYSDAI 
Sbjct: 61  KHS-YYVDLRRLGLLSIGLVCSNVTNTYHFITELIRNKNLDSSIKLCLVDCLELYSDAIS 120

Query: 123 TVKRAARDYRLKRYADANIAIGTVMDAASTCEDGFKEKKGVRSPLKKRDEDAFELGAIVL 182
           TVK A R+YRL+RY DAN+AI  VMDAA TCE+GFKEKK V SPLKKRDED FELGAIVL
Sbjct: 121 TVKLAMRNYRLRRYNDANVAISAVMDAAETCENGFKEKK-VNSPLKKRDEDVFELGAIVL 180

Query: 183 AIMNLLH 190
           AIM++LH
Sbjct: 181 AIMSMLH 185

[Close]

BLAST of Lag0016343 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.0e-28
Identity = 70/178 (39.33%), Postives = 113/178 (63.48%), Query Frame = 0


Query: 11  YILSIFFFSNFLIAQSSHNINTPLIHKTCKKIAVENPNISFKFCSTSLQSATKRSRRCAD 70
           + L IFFF+  L+ Q+   I+  ++  TCKK+A  +PN+++ FC  SL +  K     AD
Sbjct: 5   FSLCIFFFNLLLLLQAV--ISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPK--SHTAD 64

Query: 71  LRRLGLLSIGLVRSNVTSTRHLITKLMRNKSLDPFVRSCLSDCLELYSDAIPTVKRAARD 130
           L+ LG++S  L   + +  +  I +++++K +DP ++  L+DC+ LY+DA  +V+ A  D
Sbjct: 65  LQGLGVISANLAIQHGSKIQTFIGRILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIAD 124

Query: 131 YRLKRYADANIAIGTVMDAASTCEDGFKEKKGVRSPLKKRDEDAFELGAIVLAIMNLL 189
           ++ K YA AN+ +   +D + TCEDGFKEKKG+ SP+ K ++D  +L AI LAI  LL
Sbjct: 125 FKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKLL 177

[Close]

BLAST of Lag0016343 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.0e-26
Identity = 63/159 (39.62%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 30  INTPLIHKTCKKIAVENPNISFKFCSTSLQSATKRSRRCADLRRLGLLSIGLVRSNVTST 89
           I+  ++  TCKK+A  +PN+++ FC  SL +  K     ADL+ LG++S  L     +  
Sbjct: 13  ISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPK--SHSADLQGLGVISANLAIQQGSKI 72

Query: 90  RHLITKLMRNKSLDPFVRSCLSDCLELYSDAIPTVKRAARDYRLKRYADANIAIGTVMDA 149
           +  I +++++K +DP ++  L+DC+ LY+DA  +V+ A  D++ K YA AN+ +   +D 
Sbjct: 73  QTFIGRILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDD 132

Query: 150 ASTCEDGFKEKKGVRSPLKKRDEDAFELGAIVLAIMNLL 189
           + TCEDGFKEKKG+ SP+ K ++D  +L AI LAI  LL
Sbjct: 133 SVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKLL 168

The following BLAST results are available for this feature:

BLAST of Lag0016343 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0039956.1	2.1e-65	73.94	putative invertase inhibitor [Cucumis melo var. makuwa] >TYK24547.1 putative inv...	[more]
XP_008460048.1	4.6e-65	73.40	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
XP_022999945.1	1.9e-63	73.26	putative invertase inhibitor [Cucurbita maxima]	[more]
KAG6593720.1	9.7e-63	72.19	hypothetical protein SDJN03_13196, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022964389.1	1.3e-62	72.19	putative invertase inhibitor [Cucurbita moschata]	[more]

BLAST of Lag0016343 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	8.0e-28	39.33	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	2.0e-26	39.62	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..184 e-value: 1.7E-24 score: 97.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..184 e-value: 9.0E-24 score: 84.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 7..189 e-value: 1.2E-34 score: 117.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 30..189 e-value: 4.0E-34 score: 119.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..188
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 31..188
None	No IPR available	PANTHER	PTHR31080:SF226	PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN-RELATED	coord: 31..188
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 39..184 e-value: 2.92638E-42 score: 137.111

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0016343.1	Lag0016343.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity