Lag0015966 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0015966
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr12: 30363455 .. 30364202 (+)
RNA-Seq ExpressionLag0015966
SyntenyLag0015966
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTAAATCCATGTGGTGCTGCTCAAAGTTTGCATCTTTGTATCAGTCTTTATCTGATTTGAATTTTGATTCACTGCTGTGGGCTTTAAAGGATAGAGTAAGCAGGCTGGATTTTGAGCTCTTGACCGTTTTCTGGTGGGTTGTATGGAATTTATGAAACTCATTACGTGGGATGGGAATTTTGATGACCGAAATTTGATATAGTGGGTGAGGGACTACTTAGATACGTTCCAGAAGGCGTCGGGGAGAAGCTTTCAGTCTCCGGTTGGTTTGCATCATTCGCAGTCGGTGAGGGCCGTGGTGTCGTGGGTTCCGCCAGCTGTGAATGGCCTGAAACTGAATATTGATGGGTCGGTAAGGCCTGAAACTGGGGCGGCGAGCGGTGGTTTTGTCTTGCGAAATGATAGAGGGATGTTCTATTGACGACTTGTGAAATTTTGCCCAAGTGTTGGACTGCGGACTTGGCCGAGGGATGGGCAATGTTGAGAGGTATACAAATTGCTCGCCAAATGGGTTTTTTTTTCAGGTTTCATGTTGAGACCGACTCGTTGAGATTATCAAGAGTTTTGACAGACGATGTGGATGATATTTCGGAAGTAGGAGCAATTATGGAAAAGATTCGTGGCTTGCTCCAGCAAGACTCTTCATGTAGGGTGTTATTCACACCTAGGCAAGGCAACTTGGTAGCTCATACCATGACTTCTTTAGCTTTTCTTTATGCTAATTATATTTGGCTTGAGGATTAA

mRNA sequence

ATGATTAAATCCATGTGGTGCTGCTCAAAGTTTGCATCTTTGTATCAGTCTTTATCTGATTTGAATTTTGATTCACTGCTGTGGGCTTTAAAGGATAGATGGGTGAGGGACTACTTAGATACGTTCCAGAAGGCGTCGGGGAGAAGCTTTCAGTCTCCGGTTGGTTTGCATCATTCGCAGTCGGTGAGGGCCGTGGTGTCGTGGGTTCCGCCAGCTGTGAATGGCCTGAAACTGAATATTGATGGGTCGTGTTGGACTGCGGACTTGGCCGAGGGATGGGCAATGTTGAGAGGTATACAAATTGCTCGCCAAATGGGTTTTTTTTTCAGGTTTCATGTTGAGACCGACTCGTTGAGATTATCAAGAGTTTTGACAGACGATGTGGATGATATTTCGGAAGTAGGAGCAATTATGGAAAAGATTCGTGGCTTGCTCCAGCAAGACTCTTCATGTAGGGTGTTATTCACACCTAGGCAAGGCAACTTGGTAGCTCATACCATGACTTCTTTAGCTTTTCTTTATGCTAATTATATTTGGCTTGAGGATTAA

Coding sequence (CDS)

ATGATTAAATCCATGTGGTGCTGCTCAAAGTTTGCATCTTTGTATCAGTCTTTATCTGATTTGAATTTTGATTCACTGCTGTGGGCTTTAAAGGATAGATGGGTGAGGGACTACTTAGATACGTTCCAGAAGGCGTCGGGGAGAAGCTTTCAGTCTCCGGTTGGTTTGCATCATTCGCAGTCGGTGAGGGCCGTGGTGTCGTGGGTTCCGCCAGCTGTGAATGGCCTGAAACTGAATATTGATGGGTCGTGTTGGACTGCGGACTTGGCCGAGGGATGGGCAATGTTGAGAGGTATACAAATTGCTCGCCAAATGGGTTTTTTTTTCAGGTTTCATGTTGAGACCGACTCGTTGAGATTATCAAGAGTTTTGACAGACGATGTGGATGATATTTCGGAAGTAGGAGCAATTATGGAAAAGATTCGTGGCTTGCTCCAGCAAGACTCTTCATGTAGGGTGTTATTCACACCTAGGCAAGGCAACTTGGTAGCTCATACCATGACTTCTTTAGCTTTTCTTTATGCTAATTATATTTGGCTTGAGGATTAA

Protein sequence

MIKSMWCCSKFASLYQSLSDLNFDSLLWALKDRWVRDYLDTFQKASGRSFQSPVGLHHSQSVRAVVSWVPPAVNGLKLNIDGSCWTADLAEGWAMLRGIQIARQMGFFFRFHVETDSLRLSRVLTDDVDDISEVGAIMEKIRGLLQQDSSCRVLFTPRQGNLVAHTMTSLAFLYANYIWLED
Homology
BLAST of Lag0015966 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-08
Identity = 55/182 (30.22%), Postives = 76/182 (41.76%), Query Frame = 0

Query: 34  WVRDYLDTFQKASGRSFQSPVGLHHSQSVRAVVSWVPPAVNGLKLNIDGS---------- 93
           W  +YL  +Q A   S  + V     +    V  W PPA   LK+N+D +          
Sbjct: 74  WSENYLQVYQAAQRSSXSALVVCRPPR----VAIWRPPAAPLLKVNVDAAFRKESFVAGV 133

Query: 94  ---------------------CWTADLAEGWAMLRGIQIARQMGFFFRFHVETDSLRLSR 153
                                    D  EG+A+  GI +A + G F RF +ETDSLR+  
Sbjct: 134 GVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAG-FIRFQIETDSLRIFN 193

Query: 154 VLTDDVDDISEVGAIMEKIRGLLQQDSS-CRVLFTPRQGNLVAHTMTSLAFLYAN-YIWL 183
           +LT D  D SEVG +   I+  L   +      FT R GN  AH +  LA    +  IW+
Sbjct: 194 LLTTDCVDDSEVGVLCSVIKLFLSSHAERVSFSFTHRNGNAXAHLLAQLALTSPHLQIWV 250

BLAST of Lag0015966 vs. NCBI nr
Match: KAF7805280.1 (non-LTR retroelement reverse transcriptase-like [Senna tora])

HSP 1 Score: 68.6 bits (166), Expect = 6.5e-08
Identity = 48/183 (26.23%), Postives = 79/183 (43.17%), Query Frame = 0

Query: 28  WALKDRW---------VRDYLDTFQKASGRSFQSPVGLHHSQSVRAVVSWVPPAVNGLKL 87
           W  K++W          R+Y++  ++A      S     H  ++   + W PP    +KL
Sbjct: 454 WVFKNQWQGTNQIISMARNYMEGLKEAERTCIHS--NNRHISNLNQ-IKWNPPCNGWIKL 513

Query: 88  NIDGSCW------------------------------TADLAEGWAMLRGIQIARQMGFF 147
           N+DGS W                               ++LAE WA+L G+++A  M   
Sbjct: 514 NVDGSYWQHTDTISCGGVLRDEQGRWIKGFTKKMGRGNSNLAEAWAVLTGLKMAWDMK-I 573

Query: 148 FRFHVETDSLRLSRVLTDDVDDISEVGAIMEKIRGLLQQDSSCRVLFTPRQGNLVAHTMT 172
            R HVE DSL +  ++ D VD    +G I++KIR    +D +  +    R+ N VA+ + 
Sbjct: 574 DRIHVEADSLNIVNMIKDGVDVAHPLGVIVDKIREFCAKDWAVYIDHVFRESNRVANALA 632

BLAST of Lag0015966 vs. NCBI nr
Match: KAF7834439.1 (WEB family protein [Senna tora])

HSP 1 Score: 66.2 bits (160), Expect = 3.2e-07
Identity = 41/136 (30.15%), Postives = 58/136 (42.65%), Query Frame = 0

Query: 68  WVPPAVNGLKLNIDGSCWTAD------------------------------LAEGWAMLR 127
           W+PP  N  K+N+DGSCW  D                              LAE W +  
Sbjct: 767 WIPPEANMFKVNVDGSCWENDMSISCGGIIRDAEKRWVMGFAKNLGKGNVLLAEMWGIRM 826

Query: 128 GIQIARQMGFFFRFHVETDSLRLSRVLTDDVDDISEVGAIMEKIRGLLQQDSSCRVLFTP 174
           G+QIAR  G      +ETDSL   +++  +  +   + AI E I+ +L  + S  ++  P
Sbjct: 827 GLQIARNNG-LSNITIETDSLAAIKLIKGNTSESHPLYAITEDIKRMLFSNGSMNLVHIP 886

BLAST of Lag0015966 vs. NCBI nr
Match: XP_037497304.1 (uncharacterized protein LOC105643257 [Jatropha curcas])

HSP 1 Score: 63.5 bits (153), Expect = 2.1e-06
Identity = 49/167 (29.34%), Postives = 78/167 (46.71%), Query Frame = 0

Query: 19  SDLNFDSLLWALKDRWVRDYLDTFQKASGRSFQSPVGLHHSQSVRA--VVSWVPPAVNGL 78
           S  NF   L    DR + D    F  +  R + +      + SV +  V +W PP    +
Sbjct: 105 SATNFSDTLLGFIDRRIDDQCLLFATSFLREWNNMQIDRSNSSVSSALVPTWAPPPTGVV 164

Query: 79  KLNIDGSCWTADLAEGWAMLRGIQIARQMGFFF------RFH---VETDSLRLSRVLTDD 138
           K N+D +     + EG +   G+ I  ++G F        F    VETD LRL + +T  
Sbjct: 165 KSNVDAA---VHMTEGRSSYAGL-IRNEVGQFLVACTGHHFEGVLVETDCLRLFQAVTTG 224

Query: 139 VDDISEVGAIMEKIRGLLQQDSSCRVLFTPRQGNLVAHTMTSLAFLY 175
            D  S+   ++E+I+ LL Q SS  +++  RQGN+ AH +  +  L+
Sbjct: 225 FDSYSDWAPVLEEIKQLLSQISSSELVWVQRQGNMPAHALARMTCLH 267

BLAST of Lag0015966 vs. NCBI nr
Match: XP_030509082.1 (uncharacterized protein LOC115723746 [Cannabis sativa])

HSP 1 Score: 62.0 bits (149), Expect = 6.1e-06
Identity = 45/170 (26.47%), Postives = 72/170 (42.35%), Query Frame = 0

Query: 36  RDYLDTFQKASGR---SFQSPVGLHHSQSVRAVVSWVPPAVNGLKLNIDGSC-------- 95
           +DYLD +Q A  +     QS   L+       +++W PP V  LKLN D +         
Sbjct: 140 QDYLDFYQAAQDKRKTQTQSTTDLN-------LLAWEPPPVGTLKLNTDAAVSNHQNRTG 199

Query: 96  -----------------------WTADLAEGWAMLRGIQIARQMGFFFRFHVETDSLRLS 155
                                    + +AEGW +L G++ +R  GF  + H+E D   L 
Sbjct: 200 GGVLVRDHTGKVLAATAFNRMGQMQSQVAEGWVLLEGLKWSRDNGFNIQ-HIEVDCKNLL 259

Query: 156 RVLTDDVDDISEVGAIMEKIRGLLQQDSSCRVLFTPRQGNLVAHTMTSLA 172
             L    +++S  G+I+  IR +L    +  +  T RQGN  AH +  ++
Sbjct: 260 TDLQSTNENLSSYGSIINAIRRMLSSLPTVTLHHTRRQGNTAAHNLAQMS 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150944.11.7e-0830.22uncharacterized protein LOC111018973 [Momordica charantia][more]
KAF7805280.16.5e-0826.23non-LTR retroelement reverse transcriptase-like [Senna tora][more]
KAF7834439.13.2e-0730.15WEB family protein [Senna tora][more]
XP_037497304.12.1e-0629.34uncharacterized protein LOC105643257 [Jatropha curcas][more]
XP_030509082.16.1e-0626.47uncharacterized protein LOC115723746 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 82..169
e-value: 2.8E-13
score: 49.8
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 84..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0015966.1Lag0015966.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity