Lag0015577 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0015577
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase
Location	chr12: 16797171 .. 16798054 (+)
RNA-Seq Expression	Lag0015577
Synteny	Lag0015577

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAGATGCTGAGAATAGTCGAGAGGTTTCTGATTATAAGGAGAAGGAAATAGCAGGTAAAAAACACAATATTGATTTTGAGGAGGAGGGATTAATTAAGATGAAGGTGTTGGTTGTTGACGGACATAATATTGCGAGAACGGTGGAGGCTGCGGAGCAGCCCCACCGAGCACAATGAAAATATTAAGCTGGAATGTCTAGAGGCTGGGGAACCCAGGAAATTCAGAGCGCTACGTCATGTAATTTGTAGCCATCATCCTCAAATGGCTTTCTTGATGGAAACTAAACTAAAAGGAAACAAGGTTGACAAATTTAGGCGAGATTTGGATTTTGAGTATGGATTAGCTGTTTCATGCGAGGGTTTGAGTGGTGGTTTGTTCCTGTTGTGGAAAAATGATATTGATGTCACTATTAGATCTTACTCGAAAGGGCATATTGACGTTGTGATTAAAGAAAAGGATTGGCAATGGAGGTTTTTGGGTATATATGGAAATCCTAATAGGGAATTGCATCATACTACTTGGACCTTGTTACATAGGCTAAAGAATAACTGTAACCTTCCTTGGATTCTAGGGAGGATTTTAATGAAATCACAAGTCAATCTGAGAAGATGGGGGTAGGAAGCGGGCAGAATCGGAAATGCAAGAGTTTCGAGATGTTATTGATAAATGTGAGTTATATGACCCAGGGTACATCGGTGCTGATTTTACATGGTATAATAACCATGTCAACGGTGAGATGATCTGGGAGCGGTTGGACTGTTTTTTGCTGGATTATGAAATGCAAAATAAGTGCAGCTCTGTTAAAGTTTTCCACTTACCTTGCATTGCCTCAGATCATAGACCAATTTTGGCTGAATGGCAAGAGGATAAGAGAGTGTAA

mRNA sequence

ATGAGAGATGCTGAGAATAGTCGAGAGGTTTCTGATTATAAGGAGAAGGAAATAGCAGAACGGTGGAGGCTGCGGAGCAGCCCCACCGAGCACAATGAAAATATTAAGCTGGAATGTCTAGAGGCTGGGGAACCCAGGAAATTCAGAGCGCTACGTCATGTAATTTGTAGCCATCATCCTCAAATGGCTTTCTTGATGGAAACTAAACTAAAAGGAAACAAGGTTGACAAATTTAGGCGAGATTTGGATTTTGAGTATGGATTAGCTGTTTCATGCGAGGGTTTGAGTGGTGGTTTGTTCCTGTTGTGGAAAAATGATATTGATGTCACTATTAGATCTTACTCGAAAGGGCATATTGACGTTGTGATTAAAGAAAAGGATTGGCAATGGAGGAAGCGGGCAGAATCGGAAATGCAAGAGTTTCGAGATGTTATTGATAAATGTGAGTTATATGACCCAGGGTACATCGGTGCTGATTTTACATGGTATAATAACCATGTCAACGGTGAGATGATCTGGGAGCGGTTGGACTGTTTTTTGCTGGATTATGAAATGCAAAATAAGTGCAGCTCTGTTAAAGTTTTCCACTTACCTTGCATTGCCTCAGATCATAGACCAATTTTGGCTGAATGGCAAGAGGATAAGAGAGTGTAA

Coding sequence (CDS)

ATGAGAGATGCTGAGAATAGTCGAGAGGTTTCTGATTATAAGGAGAAGGAAATAGCAGAACGGTGGAGGCTGCGGAGCAGCCCCACCGAGCACAATGAAAATATTAAGCTGGAATGTCTAGAGGCTGGGGAACCCAGGAAATTCAGAGCGCTACGTCATGTAATTTGTAGCCATCATCCTCAAATGGCTTTCTTGATGGAAACTAAACTAAAAGGAAACAAGGTTGACAAATTTAGGCGAGATTTGGATTTTGAGTATGGATTAGCTGTTTCATGCGAGGGTTTGAGTGGTGGTTTGTTCCTGTTGTGGAAAAATGATATTGATGTCACTATTAGATCTTACTCGAAAGGGCATATTGACGTTGTGATTAAAGAAAAGGATTGGCAATGGAGGAAGCGGGCAGAATCGGAAATGCAAGAGTTTCGAGATGTTATTGATAAATGTGAGTTATATGACCCAGGGTACATCGGTGCTGATTTTACATGGTATAATAACCATGTCAACGGTGAGATGATCTGGGAGCGGTTGGACTGTTTTTTGCTGGATTATGAAATGCAAAATAAGTGCAGCTCTGTTAAAGTTTTCCACTTACCTTGCATTGCCTCAGATCATAGACCAATTTTGGCTGAATGGCAAGAGGATAAGAGAGTGTAA

Protein sequence

MRDAENSREVSDYKEKEIAERWRLRSSPTEHNENIKLECLEAGEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYGLAVSCEGLSGGLFLLWKNDIDVTIRSYSKGHIDVVIKEKDWQWRKRAESEMQEFRDVIDKCELYDPGYIGADFTWYNNHVNGEMIWERLDCFLLDYEMQNKCSSVKVFHLPCIASDHRPILAEWQEDKRV

Homology

[Close]

BLAST of Lag0015577 vs. NCBI nr
Match: KAA3453752.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 133.3 bits (334), Expect = 2.6e-27
Identity = 76/206 (36.89%), Postives = 106/206 (51.46%), Query Frame = 0


Query: 28  PTEHNENIKLECLEAGEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYG 87
           PT  NEN  LEC   GE    R LR++    +P++ FLMETKL   ++++ RR   F  G
Sbjct: 4   PTGRNENHMLECPWVGESTGLRRLRYLCKQQNPRIVFLMETKLDQKRMERVRRMCGFSSG 63

Query: 88  LAVSCEGLSGGLFLLWKNDIDVTIRSYSKGHIDVVIKE----KDWQWR------------ 147
           L +  EG  GGL L WK+DI VT+RSYSK HIDV++ E     D+ W             
Sbjct: 64  LEIEAEGSRGGLCLAWKDDIVVTLRSYSKWHIDVLLWEGSIQNDYPWLVAGDFNEILYSF 123

Query: 148 ------KRAESEMQEFRDVIDKCELYDPGYIGADFTWYNNHVNGEMIWERLDCFLLDYEM 207
                  R +  M+ FRD++++C+L D GY+G  +TW   ++    I ERLD  + + E 
Sbjct: 124 EKSGGVPREQKRMEAFRDILEECKLSDIGYLGVWYTWERGNLPETNIKERLDRGVANEEW 183

Query: 208 QNKCSSVKVFHLPCIASDHRPILAEW 212
                   + HL   +SDH  I   W
Sbjct: 184 LLLFPMGNIQHLTFSSSDHCLIREFW 209

[Close]

BLAST of Lag0015577 vs. NCBI nr
Match: KAA3453480.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 129.4 bits (324), Expect = 3.7e-26
Identity = 76/227 (33.48%), Postives = 102/227 (44.93%), Query Frame = 0


Query: 43  GEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYGLAVSCEGLSGGLFLL 102
           G PR  R LR+++  HHP + FLMETKL    +D+ RR   F  G+ V  EG  GGL L 
Sbjct: 12  GSPRAVRRLRYILKQHHPHLVFLMETKLDSKCMDRVRRSCGFTNGINVEAEGSRGGLCLA 71

Query: 103 WKNDIDVTIRSYSKGHIDVVIKE----------------------------------KDW 162
           W++DI VT+RSYSK HIDV+IKE                                  KD+
Sbjct: 72  WRDDIGVTLRSYSKWHIDVLIKEEGIQEEWRFTGFYGSPYVRDQKYVWNLLERLSQDKDY 131

Query: 163 QW------------------RKRAESEMQEFRDVIDKCELYDPGYIGADFTWYNNHVNGE 218
            W                  + R    M+ FRD +  C L D G+ G  FTW   +++  
Sbjct: 132 PWLVAGDFNEIMFSFEKQGGQLRDPRRMETFRDTLANCGLMDVGFSGTWFTWERGNLSET 191

[Close]

BLAST of Lag0015577 vs. NCBI nr
Match: KAA3466249.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 124.8 bits (312), Expect = 9.2e-25
Identity = 76/218 (34.86%), Postives = 100/218 (45.87%), Query Frame = 0


Query: 43  GEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYGLAVSCEGLSGGLFLL 102
           G PR  R LRH+I  H PQ  FLMETKL   ++ K RR  DF  G+ +  EG  GGL L 
Sbjct: 12  GSPRAVRRLRHLIKQHTPQFVFLMETKLSQQRMSKARRSCDFINGIDIDAEGSRGGLCLA 71

Query: 103 WKNDIDVTIRSYSKGHIDVVIKEKDWQ--WR----------------------------- 162
           WK  +D+T++S+SK HIDV++KE   Q  WR                             
Sbjct: 72  WKPGMDITLKSFSKWHIDVMVKEDSSQAKWRFTGFYGTPYSRDQNQVWDLLKSLSQNGTG 131

Query: 163 ---------------------KRAESEMQEFRDVIDKCELYDPGYIGADFTWYNNHVNGE 209
                                 R +  M+ FR+ + KC+L D G+ GA FTW   ++   
Sbjct: 132 PWLVAEDFNEIMYSFKKKRGLPRDQRRMEIFRETLAKCQLADIGFSGASFTWERGNLLET 191

[Close]

BLAST of Lag0015577 vs. NCBI nr
Match: XP_022157437.1 (uncharacterized protein LOC111024135 [Momordica charantia])

HSP 1 Score: 123.6 bits (309), Expect = 2.0e-24
Identity = 73/195 (37.44%), Postives = 102/195 (52.31%), Query Frame = 0


Query: 43  GEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYGLAVSCEGLSGGLFLL 102
           G P  FR LR+++    PQ+ FL ETK       + +R+L F+  ++V+  G SGGL LL
Sbjct: 12  GNPDTFRTLRNLVRRSQPQLVFLSETKGNPTLEGRAKRELSFDCCVSVASTGKSGGLMLL 71

Query: 103 WKNDIDVTIRSYSKGHIDVVIKEKDWQWR-----------KRAES--EMQEFRDVIDKCE 162
           W +D +V I+S S GHID +I +K   WR           KR+ S   ++    ++D   
Sbjct: 72  WNSDSNVRIQSMSPGHIDSIITDKYGSWRFTGFYGNPCTYKRSASWKLLERLARMMDL-- 131

Query: 163 LYDPGYIGADFTWY-------------NNHVNGEMIWERLDCFLLDYEMQNKCSSVKVFH 212
              P  IG DF                 + + G  IWERLD FL++  M NKC ++KV H
Sbjct: 132 ---PWIIGGDFNEIVSMTEKMGGVCRNESQMRGCPIWERLDRFLINESMLNKCLNLKVTH 191

[Close]

BLAST of Lag0015577 vs. NCBI nr
Match: KAA3484396.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 123.6 bits (309), Expect = 2.0e-24
Identity = 76/227 (33.48%), Postives = 104/227 (45.81%), Query Frame = 0


Query: 43  GEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYGLAVSCEGLSGGLFLL 102
           G  R  R LR +I  HHPQ+ FLMETKL   ++   RR   F++G+ V  EG  GGL L 
Sbjct: 12  GSSRAVRRLRFLIKQHHPQLVFLMETKLNKQRMGTVRRRCGFDFGIEVEAEGSKGGLCLA 71

Query: 103 WKNDIDVTIRSYSKGHIDVVIKEKD----------------------WQWRKRAESE--- 162
           WK D+DV ++S+SK HIDV+IKE++                      W   KR   E   
Sbjct: 72  WKKDLDVKLKSFSKWHIDVLIKEENVEEDWKFTGFYGSPYSKDKNLVWDLLKRLAQESDY 131

Query: 163 ---------------------------MQEFRDVIDKCELYDPGYIGADFTWYNNHVNGE 218
                                      M+ FR+ +  C+LYD G+ G  +TW   ++   
Sbjct: 132 PWLVEGDFNEILYSFEKCGGIPRDSKRMEVFRETLVDCQLYDIGFSGVSYTWERGNLPET 191

[Close]

BLAST of Lag0015577 vs. ExPASy TrEMBL
Match: A0A5B6UC75 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_009754 PE=4 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 76/206 (36.89%), Postives = 106/206 (51.46%), Query Frame = 0


Query: 28  PTEHNENIKLECLEAGEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYG 87
           PT  NEN  LEC   GE    R LR++    +P++ FLMETKL   ++++ RR   F  G
Sbjct: 4   PTGRNENHMLECPWVGESTGLRRLRYLCKQQNPRIVFLMETKLDQKRMERVRRMCGFSSG 63

Query: 88  LAVSCEGLSGGLFLLWKNDIDVTIRSYSKGHIDVVIKE----KDWQWR------------ 147
           L +  EG  GGL L WK+DI VT+RSYSK HIDV++ E     D+ W             
Sbjct: 64  LEIEAEGSRGGLCLAWKDDIVVTLRSYSKWHIDVLLWEGSIQNDYPWLVAGDFNEILYSF 123

Query: 148 ------KRAESEMQEFRDVIDKCELYDPGYIGADFTWYNNHVNGEMIWERLDCFLLDYEM 207
                  R +  M+ FRD++++C+L D GY+G  +TW   ++    I ERLD  + + E 
Sbjct: 124 EKSGGVPREQKRMEAFRDILEECKLSDIGYLGVWYTWERGNLPETNIKERLDRGVANEEW 183

Query: 208 QNKCSSVKVFHLPCIASDHRPILAEW 212
                   + HL   +SDH  I   W
Sbjct: 184 LLLFPMGNIQHLTFSSSDHCLIREFW 209

[Close]

BLAST of Lag0015577 vs. ExPASy TrEMBL
Match: A0A5B6U6G4 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_009510 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.8e-26
Identity = 76/227 (33.48%), Postives = 102/227 (44.93%), Query Frame = 0


Query: 43  GEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYGLAVSCEGLSGGLFLL 102
           G PR  R LR+++  HHP + FLMETKL    +D+ RR   F  G+ V  EG  GGL L 
Sbjct: 12  GSPRAVRRLRYILKQHHPHLVFLMETKLDSKCMDRVRRSCGFTNGINVEAEGSRGGLCLA 71

Query: 103 WKNDIDVTIRSYSKGHIDVVIKE----------------------------------KDW 162
           W++DI VT+RSYSK HIDV+IKE                                  KD+
Sbjct: 72  WRDDIGVTLRSYSKWHIDVLIKEEGIQEEWRFTGFYGSPYVRDQKYVWNLLERLSQDKDY 131

Query: 163 QW------------------RKRAESEMQEFRDVIDKCELYDPGYIGADFTWYNNHVNGE 218
            W                  + R    M+ FRD +  C L D G+ G  FTW   +++  
Sbjct: 132 PWLVAGDFNEIMFSFEKQGGQLRDPRRMETFRDTLANCGLMDVGFSGTWFTWERGNLSET 191

[Close]

BLAST of Lag0015577 vs. ExPASy TrEMBL
Match: A0A5B6VAT3 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_001357 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 4.4e-25
Identity = 76/218 (34.86%), Postives = 100/218 (45.87%), Query Frame = 0


Query: 43  GEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYGLAVSCEGLSGGLFLL 102
           G PR  R LRH+I  H PQ  FLMETKL   ++ K RR  DF  G+ +  EG  GGL L 
Sbjct: 12  GSPRAVRRLRHLIKQHTPQFVFLMETKLSQQRMSKARRSCDFINGIDIDAEGSRGGLCLA 71

Query: 103 WKNDIDVTIRSYSKGHIDVVIKEKDWQ--WR----------------------------- 162
           WK  +D+T++S+SK HIDV++KE   Q  WR                             
Sbjct: 72  WKPGMDITLKSFSKWHIDVMVKEDSSQAKWRFTGFYGTPYSRDQNQVWDLLKSLSQNGTG 131

Query: 163 ---------------------KRAESEMQEFRDVIDKCELYDPGYIGADFTWYNNHVNGE 209
                                 R +  M+ FR+ + KC+L D G+ GA FTW   ++   
Sbjct: 132 PWLVAEDFNEIMYSFKKKRGLPRDQRRMEIFRETLAKCQLADIGFSGASFTWERGNLLET 191

[Close]

BLAST of Lag0015577 vs. ExPASy TrEMBL
Match: A0A6J1DUG8 (uncharacterized protein LOC111024135 OS=Momordica charantia OX=3673 GN=LOC111024135 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 9.9e-25
Identity = 73/195 (37.44%), Postives = 102/195 (52.31%), Query Frame = 0


Query: 43  GEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYGLAVSCEGLSGGLFLL 102
           G P  FR LR+++    PQ+ FL ETK       + +R+L F+  ++V+  G SGGL LL
Sbjct: 12  GNPDTFRTLRNLVRRSQPQLVFLSETKGNPTLEGRAKRELSFDCCVSVASTGKSGGLMLL 71

Query: 103 WKNDIDVTIRSYSKGHIDVVIKEKDWQWR-----------KRAES--EMQEFRDVIDKCE 162
           W +D +V I+S S GHID +I +K   WR           KR+ S   ++    ++D   
Sbjct: 72  WNSDSNVRIQSMSPGHIDSIITDKYGSWRFTGFYGNPCTYKRSASWKLLERLARMMDL-- 131

Query: 163 LYDPGYIGADFTWY-------------NNHVNGEMIWERLDCFLLDYEMQNKCSSVKVFH 212
              P  IG DF                 + + G  IWERLD FL++  M NKC ++KV H
Sbjct: 132 ---PWIIGGDFNEIVSMTEKMGGVCRNESQMRGCPIWERLDRFLINESMLNKCLNLKVTH 191

[Close]

BLAST of Lag0015577 vs. ExPASy TrEMBL
Match: A0A5B6WSX4 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_006480 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 9.9e-25
Identity = 76/227 (33.48%), Postives = 104/227 (45.81%), Query Frame = 0


Query: 43  GEPRKFRALRHVICSHHPQMAFLMETKLKGNKVDKFRRDLDFEYGLAVSCEGLSGGLFLL 102
           G  R  R LR +I  HHPQ+ FLMETKL   ++   RR   F++G+ V  EG  GGL L 
Sbjct: 12  GSSRAVRRLRFLIKQHHPQLVFLMETKLNKQRMGTVRRRCGFDFGIEVEAEGSKGGLCLA 71

Query: 103 WKNDIDVTIRSYSKGHIDVVIKEKD----------------------WQWRKRAESE--- 162
           WK D+DV ++S+SK HIDV+IKE++                      W   KR   E   
Sbjct: 72  WKKDLDVKLKSFSKWHIDVLIKEENVEEDWKFTGFYGSPYSKDKNLVWDLLKRLAQESDY 131

Query: 163 ---------------------------MQEFRDVIDKCELYDPGYIGADFTWYNNHVNGE 218
                                      M+ FR+ +  C+LYD G+ G  +TW   ++   
Sbjct: 132 PWLVEGDFNEILYSFEKCGGIPRDSKRMEVFRETLVDCQLYDIGFSGVSYTWERGNLPET 191

The following BLAST results are available for this feature:

BLAST of Lag0015577 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA3453752.1	2.6e-27	36.89	reverse transcriptase [Gossypium australe]	[more]
KAA3453480.1	3.7e-26	33.48	reverse transcriptase [Gossypium australe]	[more]
KAA3466249.1	9.2e-25	34.86	reverse transcriptase [Gossypium australe]	[more]
XP_022157437.1	2.0e-24	37.44	uncharacterized protein LOC111024135 [Momordica charantia]	[more]
KAA3484396.1	2.0e-24	33.48	reverse transcriptase [Gossypium australe]	[more]

BLAST of Lag0015577 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0015577 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5B6UC75	1.2e-27	36.89	Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_009754 PE=4 SV=1	[more]
A0A5B6U6G4	1.8e-26	33.48	Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_009510 PE=4 SV=1	[more]
A0A5B6VAT3	4.4e-25	34.86	Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_001357 PE=4 SV=1	[more]
A0A6J1DUG8	9.9e-25	37.44	uncharacterized protein LOC111024135 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A5B6WSX4	9.9e-25	33.48	Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_006480 PE=4 SV=1	[more]

BLAST of Lag0015577 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036691	Endonuclease/exonuclease/phosphatase superfamily	GENE3D	3.60.10.10	Endonuclease/exonuclease/phosphatase	coord: 117..210 e-value: 3.6E-10 score: 42.0
IPR036691	Endonuclease/exonuclease/phosphatase superfamily	SUPERFAMILY	56219	DNase I-like	coord: 45..210
None	No IPR available	PANTHER	PTHR33710:SF37	SUBFAMILY NOT NAMED	coord: 72..209
None	No IPR available	PANTHER	PTHR33710	BNAC02G09200D PROTEIN	coord: 72..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0015577.1	Lag0015577.1	mRNA