Lag0015133 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0015133
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRING-CH-type domain-containing protein
Locationchr12: 7957599 .. 7958365 (-)
RNA-Seq ExpressionLag0015133
SyntenyLag0015133
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCAAACGAGAGGAAGGATCGAGTGGGACTACGAGTCCAGACGAACCTGAGACAGTTGTCGTATTGCCATCGAGACCTGAAGAAGCAAAAGAAGTAATTGGAGGGACGGACGGGAACCATGCGCACGAGAATGAGGGGACTGGCAAGAGGCATGGAGAGGAAACGATAGAGAGCTCTAGACAGCCTGAGAGCCACCATAACTGCATCATTGATATAAAAAGCAATGGTGTGACAGAGGATGATGAAGATTCTAATGAAGAAAATGTTTGCAGGATATGTCATTTGGGCTCTGAACACCAGTCAATAACTTCAGATCTCATACACCTCGGATGTCACTGCAAAGACGAGCTGGGAATTTCCCACCGTGACTGCGCCGAGATTTGGTTTATGCACAGAGGCAACAGGTAAAATTTCTCTGGTCTTCATCCTCTACTCTCTTCACCATTCTGATATCAGTTATGTTGCACTTCTAACTCCAATCCAATTTCATCCCTTTCTAATGATAGACAGTGTGAGATCTGTGGTCAGACTGCAACAAACGTGAGGGGAAACCGGAGTAGCGGTTTCACAATACAGCGGAATGAAAGGAGGTTGGTAGCAAGCTCAACCGTGTCTACAGTGTCCTTGGACAACGATGTTTCATTATCATGTTGCTGGGGGCAGCGCTTCTGTAATTTTCTATTAACATGCACAGTTCTAGCCTTCCTCCTTCCATGGCTTTTTCGTCTAGATTTCTTCGGATGATGAGTGATGGCTTTTCTTGA

mRNA sequence

ATGATCAAACGAGAGGAAGGATCGAGTGGGACTACGAGTCCAGACGAACCTGAGACAGTTGTCGTATTGCCATCGAGACCTGAAGAAGCAAAAGAAGTAATTGGAGGGACGGACGGGAACCATGCGCACGAGAATGAGGGGACTGGCAAGAGGCATGGAGAGGAAACGATAGAGAGCTCTAGACAGCCTGAGAGCCACCATAACTGCATCATTGATATAAAAAGCAATGGTGTGACAGAGGATGATGAAGATTCTAATGAAGAAAATGTTTGCAGGATATGTCATTTGGGCTCTGAACACCAGTCAATAACTTCAGATCTCATACACCTCGGATGTCACTGCAAAGACGAGCTGGGAATTTCCCACCGTGACTGCGCCGAGATTTGGTTTATGCACAGAGGCAACAGACAGTGTGAGATCTGTGGTCAGACTGCAACAAACGTGAGGGGAAACCGGAGTAGCGGTTTCACAATACAGCGGAATGAAAGGAGTTCTAGCCTTCCTCCTTCCATGGCTTTTTCGTCTAGATTTCTTCGGATGATGAGTGATGGCTTTTCTTGA

Coding sequence (CDS)

ATGATCAAACGAGAGGAAGGATCGAGTGGGACTACGAGTCCAGACGAACCTGAGACAGTTGTCGTATTGCCATCGAGACCTGAAGAAGCAAAAGAAGTAATTGGAGGGACGGACGGGAACCATGCGCACGAGAATGAGGGGACTGGCAAGAGGCATGGAGAGGAAACGATAGAGAGCTCTAGACAGCCTGAGAGCCACCATAACTGCATCATTGATATAAAAAGCAATGGTGTGACAGAGGATGATGAAGATTCTAATGAAGAAAATGTTTGCAGGATATGTCATTTGGGCTCTGAACACCAGTCAATAACTTCAGATCTCATACACCTCGGATGTCACTGCAAAGACGAGCTGGGAATTTCCCACCGTGACTGCGCCGAGATTTGGTTTATGCACAGAGGCAACAGACAGTGTGAGATCTGTGGTCAGACTGCAACAAACGTGAGGGGAAACCGGAGTAGCGGTTTCACAATACAGCGGAATGAAAGGAGTTCTAGCCTTCCTCCTTCCATGGCTTTTTCGTCTAGATTTCTTCGGATGATGAGTGATGGCTTTTCTTGA

Protein sequence

MIKREEGSSGTTSPDEPETVVVLPSRPEEAKEVIGGTDGNHAHENEGTGKRHGEETIESSRQPESHHNCIIDIKSNGVTEDDEDSNEENVCRICHLGSEHQSITSDLIHLGCHCKDELGISHRDCAEIWFMHRGNRQCEICGQTATNVRGNRSSGFTIQRNERSSSLPPSMAFSSRFLRMMSDGFS
Homology
BLAST of Lag0015133 vs. NCBI nr
Match: XP_038887762.1 (probable E3 ubiquitin ligase SUD1 [Benincasa hispida])

HSP 1 Score: 259.6 bits (662), Expect = 2.0e-65
Identity = 128/163 (78.53%), Postives = 137/163 (84.05%), Query Frame = 0

Query: 1   MIKREEGSSGTTSPDEPETVVVLPSRPEEAKEVIGGTDGNHAHENEGTGKRHGEETIESS 60
           MIK++EGSSG TSP+E  T+   PSRP EAK VIGG   N  HENE  GKRHGEE  ESS
Sbjct: 1   MIKQDEGSSGITSPNESGTIAASPSRPHEAKGVIGGESENRVHENEEDGKRHGEEMQESS 60

Query: 61  RQPESHHNCIIDIKSNGVTEDDEDSNEENVCRICHLGSEHQSITSDLIHLGCHCKDELGI 120
           RQPES+ NCIIDIKSNG + D EDS+EE VCRICHL SEH+SITSDLIHLGC CKDELGI
Sbjct: 61  RQPESNQNCIIDIKSNGGSMDGEDSSEEKVCRICHLSSEHESITSDLIHLGCSCKDELGI 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRGNRSSGFTIQRNER 164
           SHRDCAEIWFMHRGNRQCEICGQTATNVR NRSSGF IQ+NER
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSGFAIQQNER 163

BLAST of Lag0015133 vs. NCBI nr
Match: XP_008463280.1 (PREDICTED: probable E3 ubiquitin ligase SUD1 [Cucumis melo])

HSP 1 Score: 256.1 bits (653), Expect = 2.3e-64
Identity = 127/163 (77.91%), Postives = 136/163 (83.44%), Query Frame = 0

Query: 1   MIKREEGSSGTTSPDEPETVVVLPSRPEEAKEVIGGTDGNHAHENEGTGKRHGEETIESS 60
           MI++ EGSSG TSP+EPET+   PSRP EAK VIGG  G +  ENE   KRHGEE +ESS
Sbjct: 1   MIEQNEGSSGITSPNEPETIAASPSRPREAKGVIGGEGGTYVRENEENSKRHGEEMLESS 60

Query: 61  RQPESHHNCIIDIKSNGVTEDDEDSNEENVCRICHLGSEHQSITSDLIHLGCHCKDELGI 120
            QPES  NCIIDIKSNG + DDEDS+EE VCRICHLGSEH+SITSDLIHLGC CKDELGI
Sbjct: 61  -QPESRQNCIIDIKSNGESVDDEDSSEEKVCRICHLGSEHESITSDLIHLGCSCKDELGI 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRGNRSSGFTIQRNER 164
           SHRDCAEIWFMHRGNRQCEICGQTATNVR NRSS F I RNER
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNER 162

BLAST of Lag0015133 vs. NCBI nr
Match: XP_011653704.1 (probable E3 ubiquitin ligase SUD1 isoform X1 [Cucumis sativus] >KGN64779.1 hypothetical protein Csa_013679 [Cucumis sativus])

HSP 1 Score: 253.4 bits (646), Expect = 1.5e-63
Identity = 124/163 (76.07%), Postives = 136/163 (83.44%), Query Frame = 0

Query: 1   MIKREEGSSGTTSPDEPETVVVLPSRPEEAKEVIGGTDGNHAHENEGTGKRHGEETIESS 60
           MI++ EGSSG TSPDEP+T+V  PSRP EAK VIGG  G    ENE   KRHG E +E+S
Sbjct: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENRKRHGVEMLETS 60

Query: 61  RQPESHHNCIIDIKSNGVTEDDEDSNEENVCRICHLGSEHQSITSDLIHLGCHCKDELGI 120
            QPESH NCIIDIK+NG + DDEDS+EE VCRICHLGSE++SITS+LIHLGC CKDELGI
Sbjct: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRGNRSSGFTIQRNER 164
           SHRDCAEIWFMHRGNRQCEICGQTATNVR NRSS F I RNER
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNER 163

BLAST of Lag0015133 vs. NCBI nr
Match: XP_022147939.1 (uncharacterized protein LOC111016745 [Momordica charantia])

HSP 1 Score: 235.0 bits (598), Expect = 5.4e-58
Identity = 122/163 (74.85%), Postives = 132/163 (80.98%), Query Frame = 0

Query: 1   MIKREEGSSGTTSPDEPETVVVLPSRPEEAKEVIGGTDGNHAHENEGTGKRHGEETIESS 60
           MIK++EGSSGT + DE  TV+ LP RP+EAKEV GG   NH +ENE  GKRHGEE+    
Sbjct: 1   MIKQDEGSSGTPNRDESVTVLALPPRPQEAKEVTGGVVQNH-NENE-AGKRHGEES---- 60

Query: 61  RQPESHHNCIIDIKSNGVTEDDEDSNEENVCRICHLGSEHQSITSDLIHLGCHCKDELGI 120
              ESHHNCIIDIKS+G + D EDS+EE VCRICHL SEHQSI SDLI LGC CKDELGI
Sbjct: 61  --KESHHNCIIDIKSSGESVDGEDSSEEKVCRICHLSSEHQSIASDLIQLGCRCKDELGI 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRGNRSSGFTIQRNER 164
           SHRDCAEIWFMHRGNRQCEICGQTATNVRG RS GF IQRNER
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRGIRSGGFMIQRNER 155

BLAST of Lag0015133 vs. NCBI nr
Match: XP_022988953.1 (uncharacterized protein LOC111486158 isoform X1 [Cucurbita maxima])

HSP 1 Score: 232.3 bits (591), Expect = 3.5e-57
Identity = 116/163 (71.17%), Postives = 134/163 (82.21%), Query Frame = 0

Query: 1   MIKREEGSSGTTSPDEPETVVVLPSRPEEAKEVIGGTDGNHAHENEGTGKRHGEETIESS 60
           MI++EEGSS TTSPDEPE V+VLP+RPEEAKEV+  ++ +H HE+EG GK        SS
Sbjct: 1   MIEQEEGSSRTTSPDEPEIVIVLPTRPEEAKEVLARSERSHEHEHEGAGK--------SS 60

Query: 61  RQPESHHNCIIDIKSNGVTEDDEDSNEENVCRICHLGSEHQSITSDLIHLGCHCKDELGI 120
           RQPES H CIIDIKS G + DD +S++E VCRICHL S+ QS+TSDL++LGC CK ELG+
Sbjct: 61  RQPESQHTCIIDIKSTGESMDDANSSDEKVCRICHLSSD-QSVTSDLVNLGCRCKGELGM 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRGNRSSGFTIQRNER 164
           SHRDCAEIWF HRGNRQCEICGQTATNVRGNRSS F IQRNER
Sbjct: 121 SHRDCAEIWFTHRGNRQCEICGQTATNVRGNRSSVFMIQRNER 154

BLAST of Lag0015133 vs. ExPASy Swiss-Prot
Match: Q68FA7 (E3 ubiquitin-protein ligase MARCHF3 OS=Xenopus tropicalis OX=8364 GN=marchf3 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 9.4e-05
Identity = 21/56 (37.50%), Postives = 26/56 (46.43%), Query Frame = 0

Query: 86  NEENVCRICHLGSEHQSITSDLIHLGCHCKDELGISHRDCAEIWFMHRGNRQCEIC 142
           N+  +CRICH GS  + + S      C C   LG  HR C E W        CE+C
Sbjct: 64  NDRPMCRICHEGSTQEDLLSP-----CECTGTLGTIHRSCLEHWLSSSNTSYCELC 114

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887762.12.0e-6578.53probable E3 ubiquitin ligase SUD1 [Benincasa hispida][more]
XP_008463280.12.3e-6477.91PREDICTED: probable E3 ubiquitin ligase SUD1 [Cucumis melo][more]
XP_011653704.11.5e-6376.07probable E3 ubiquitin ligase SUD1 isoform X1 [Cucumis sativus] >KGN64779.1 hypot... [more]
XP_022147939.15.4e-5874.85uncharacterized protein LOC111016745 [Momordica charantia][more]
XP_022988953.13.5e-5771.17uncharacterized protein LOC111486158 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q68FA79.4e-0537.50E3 ubiquitin-protein ligase MARCHF3 OS=Xenopus tropicalis OX=8364 GN=marchf3 PE=... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 90..142
e-value: 2.4E-10
score: 50.4
IPR011016Zinc finger, RING-CH-typePFAMPF12906RINGvcoord: 91..141
e-value: 3.8E-8
score: 33.3
IPR011016Zinc finger, RING-CH-typePROSITEPS51292ZF_RING_CHcoord: 83..148
score: 18.78487
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 74..154
e-value: 8.9E-16
score: 59.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..67
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..67
NoneNo IPR availablePANTHERPTHR46214ZINC FINGER, RING-CH-TYPEcoord: 8..165
NoneNo IPR availablePANTHERPTHR46214:SF8OS01G0731800 PROTEINcoord: 8..165
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 85..145

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0015133.1Lag0015133.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008270 zinc ion binding