Lag0015118 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0015118
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	E3 ubiquitin-protein ligase EL5
Location	chr12: 7829850 .. 7831567 (+)
RNA-Seq Expression	Lag0015118
Synteny	Lag0015118

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAGACGTTCTACTAATTTTCCCCCAGGAATTTGGCAGGCTAGAAGGGAATATGATCGCCAGAGATATAACAGAAATGATTCAAACCGTATGAACTCCACACCACCAAACCCTCGTTCATTTCAGGTCAGCAGGTTAACAATTCTTTTGTTTTGTAATGAGCTGTATTCAAACCTGCCATCAAAGCTTAAGCTACTATCCTACAACATGATTAGCAGGCATAAAAACAGAACTTGTAGATTACATTTAAATTCCATGTTAAAAGTTCTCCTAGTTTTTCTCAGAAACATATTAGTAATTGAGAAGTTAAAGAGATGATAACAATCGTATAGCTAGCTCATAATTCATGTGTTGTCAGAATTCTCATAGAGAGATAGATCCAGTTCCATGGCAGCCTGCTGGAGAGATAGATCCAGTTCCGTGGCAGCCTGTTGGGGAGATAGATCCAGTTCCGTGGCGGCCAGCTGAGGGGGCAATGATTACAAGAATATTGGACATCGGCCGGTAAATAAAACATAAGCTCAGTAGTTTTATAGGTGCTGTTTGTTTTGGACTGAATTATATGAAAACCAAAGAAGTCGATATTGCAGATTCATTTCATAAAATGATCTTAAATTATAAGAATTTAGAGGCCAAGCAAAGTTTGCGGTATATTGCCTGAACTAGTATCTAACTCAAGAAGATAACTTGACAGAGCAGTCAGAACTCCATGAATTTCACTAATGGAAGAACTTGAGGAAACAAATCATGCAAATCATGAAGTGTCAGAAATGGGGTTGAGCTTCAGGTTCCTTCTTAGTTGGAGACTAGTTCGTTGAAGATTCTTTTACACAAGGGAGTTCAACAGCATTCAACACCACACAACGTATTAAGCATAAATTTGCTCACTAATAATCTCTATTTACAAGTTGCAGGGAAAAAGAAACATGTTATTCTCATTCATTTAATTGAATTGCTTATGATCAATAGCTTTCAATTTATGTCATTAGCAGGATCCAATTTTGCAATAACAGACTAATCCCTTTTGAAAATATCAGTTCGTTTTAGAGATGATCTACTTACATTGCTCTTATTACCTGCAATTAAAATCCGTATCCTCGGTACTGTGTTGATATTCATTCACTTTTTGAGATATTTCCAGCCCGCCGGAAGAACCAACTGCCATAGTCAGAATCATTACCCAATCCAGACCGAGGCAGCTCGAAGCCAGTCCAAGACTTGCGGAACTGCACTCGCCAACTCCAAGATCTCGAGGAAACTCCACGCTAACACCTGAAGAGCAAAATATGGCACTGAATAAGCTCAACAAAAAAGTCTATGACCCTCCGAATAACTCAAGTAGGAAAATAAGCTTCTATTACAGAGAGAATAACAGAAGAAATTACAACGAGAGGAGCAACGGCAACAACGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAAGTGATGACGACGCCCTGCAACCATATGTTTCACGAAGAATGCATAGTGCCATGGGTGAAGAGCAATGGCCAATGTCCAGTCTGCAGATTCGCCTTGTTCGAACGAACAGAGCGAAGAGAAGCTTCGTCCAATAATACTGTCTCAATTGCGCCGGCGAGAAGCCTGATCGACGACGAGCTTGTGTACTTGATACGAGCCATGGAAGATGCTCTGATGCTGCGAAATCTGGCCGGATTTTGGGGACCATGA

mRNA sequence

ATGAGCAGACGTTCTACTAATTTTCCCCCAGGAATTTGGCAGGCTAGAAGGGAATATGATCGCCAGAGATATAACAGAAATGATTCAAACCGTATGAACTCCACACCACCAAACCCTCGTTCATTTCAGAATTCTCATAGAGAGATAGATCCAGTTCCATGGCAGCCTGCTGGAGAGATAGATCCAGTTCCGTGGCAGCCTGTTGGGGAGATAGATCCAGTTCCGTGGCGGCCAGCTGAGGGGGCAATGATTACAAGAATATTGGACATCGGCCGCCCGCCGGAAGAACCAACTGCCATAGTCAGAATCATTACCCAATCCAGACCGAGGCAGCTCGAAGCCAGTCCAAGACTTGCGGAACTGCACTCGCCAACTCCAAGATCTCGAGGAAACTCCACGCTAACACCTGAAGAGCAAAATATGGCACTGAATAAGCTCAACAAAAAAGTCTATGACCCTCCGAATAACTCAAGTAGGAAAATAAGCTTCTATTACAGAGAGAATAACAGAAGAAATTACAACGAGAGGAGCAACGGCAACAACGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAAGTGATGACGACGCCCTGCAACCATATGTTTCACGAAGAATGCATAGTGCCATGGGTGAAGAGCAATGGCCAATGTCCAGTCTGCAGATTCGCCTTGTTCGAACGAACAGAGCGAAGAGAAGCTTCGTCCAATAATACTGTCTCAATTGCGCCGGCGAGAAGCCTGATCGACGACGAGCTTGTGTACTTGATACGAGCCATGGAAGATGCTCTGATGCTGCGAAATCTGGCCGGATTTTGGGGACCATGA

Coding sequence (CDS)

ATGAGCAGACGTTCTACTAATTTTCCCCCAGGAATTTGGCAGGCTAGAAGGGAATATGATCGCCAGAGATATAACAGAAATGATTCAAACCGTATGAACTCCACACCACCAAACCCTCGTTCATTTCAGAATTCTCATAGAGAGATAGATCCAGTTCCATGGCAGCCTGCTGGAGAGATAGATCCAGTTCCGTGGCAGCCTGTTGGGGAGATAGATCCAGTTCCGTGGCGGCCAGCTGAGGGGGCAATGATTACAAGAATATTGGACATCGGCCGCCCGCCGGAAGAACCAACTGCCATAGTCAGAATCATTACCCAATCCAGACCGAGGCAGCTCGAAGCCAGTCCAAGACTTGCGGAACTGCACTCGCCAACTCCAAGATCTCGAGGAAACTCCACGCTAACACCTGAAGAGCAAAATATGGCACTGAATAAGCTCAACAAAAAAGTCTATGACCCTCCGAATAACTCAAGTAGGAAAATAAGCTTCTATTACAGAGAGAATAACAGAAGAAATTACAACGAGAGGAGCAACGGCAACAACGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAAGTGATGACGACGCCCTGCAACCATATGTTTCACGAAGAATGCATAGTGCCATGGGTGAAGAGCAATGGCCAATGTCCAGTCTGCAGATTCGCCTTGTTCGAACGAACAGAGCGAAGAGAAGCTTCGTCCAATAATACTGTCTCAATTGCGCCGGCGAGAAGCCTGATCGACGACGAGCTTGTGTACTTGATACGAGCCATGGAAGATGCTCTGATGCTGCGAAATCTGGCCGGATTTTGGGGACCATGA

Protein sequence

MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEIDPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAELHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGNNDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERREASSNNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWGP

Homology

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BLAST of Lag0015118 vs. NCBI nr
Match: XP_023529368.1 (E3 ubiquitin-protein ligase EL5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 450.3 bits (1157), Expect = 1.2e-122
Identity = 218/281 (77.58%), Postives = 244/281 (86.83%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRRSTNFPPG+W+ARREYD QRYN NDSNRM+ST P+P+ FQNS R+ +P+P +P G I
Sbjct: 1   MSRRSTNFPPGLWRARREYDHQRYNTNDSNRMSSTSPHPQPFQNSSRDTNPIPLRPTGGI 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
           DPVP +P  E+DP+PWRP  GAMI R+L+I    E+P AIVRIIT SRPRQ+EAS R AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           ++ PTPRS GNSTLT EEQNMALNKL KKVYDPPNN+SR+ISFYYRENNR+NYNERSNG 
Sbjct: 121 MYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRKNYNERSNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWG 282
           ASSNNT SIA   +L+DDELVYLIRAMED+LMLRNL G WG
Sbjct: 241 ASSNNTASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

[Close]

BLAST of Lag0015118 vs. NCBI nr
Match: XP_022989128.1 (E3 ubiquitin-protein ligase EL5 [Cucurbita maxima])

HSP 1 Score: 449.1 bits (1154), Expect = 2.8e-122
Identity = 219/281 (77.94%), Postives = 243/281 (86.48%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRRSTNFPPG+W+ARREYDRQRYN NDSNRM+ST P+P+ FQNS R+ +P+P +P   I
Sbjct: 1   MSRRSTNFPPGLWRARREYDRQRYNTNDSNRMSSTSPHPQPFQNSSRDTEPIPSRPTRGI 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
           DPVP +P  E+DP+PWRP  GAMI R+L+I    E+P AIVRIIT SRPRQ+EAS R AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           L+ PTPRS GNSTLT EEQNMALNKL KKVYDPPNN+SR+ISFYYRENNRRNYNER NG 
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERRNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWG 282
           ASSNNT SIA   +L+DDELVYLIRAMED+LMLRNL G WG
Sbjct: 241 ASSNNTASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

[Close]

BLAST of Lag0015118 vs. NCBI nr
Match: XP_022930836.1 (E3 ubiquitin-protein ligase EL5 [Cucurbita moschata] >KAG7022947.1 E3 ubiquitin-protein ligase SDIR1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 448.0 bits (1151), Expect = 6.1e-122
Identity = 218/281 (77.58%), Postives = 242/281 (86.12%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRR TNFPPG+W+ARREYD QRYN NDSNRM+ST P+P+ FQNS R+ +P+P +P G I
Sbjct: 1   MSRRPTNFPPGLWRARREYDHQRYNTNDSNRMSSTSPHPQPFQNSSRDTNPIPSRPTGGI 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
           DPVP +P  E+DP+PWRP  GAMI R+L+I    E+P AIVRIIT SRPRQ+EAS R AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           L+ PTPRS GNSTLT EEQNMALNKL KKVYDPPNN+SR+ISFYYRENNRRNYNERSNG 
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERSNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWG 282
           ASSNN  SIA   +L+DDELVYLIRAMED+LMLRNL G WG
Sbjct: 241 ASSNNAASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

[Close]

BLAST of Lag0015118 vs. NCBI nr
Match: XP_038888228.1 (uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida])

HSP 1 Score: 437.6 bits (1124), Expect = 8.3e-119
Identity = 224/283 (79.15%), Postives = 237/283 (83.75%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRRSTNFPPGIWQARREYDRQRYNRNDSNR+NSTP +  SFQNS+RE+DPVPW+PAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRINSTPSHTNSFQNSYREVDPVPWRPAGE- 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
                                 MI RILDIGRP EEP AIVRIIT SRPRQLEASP  AE
Sbjct: 61  ---------------------TMIARILDIGRPREEPPAIVRIITSSRPRQLEASPIPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           L+SPTPRS GNSTLT EEQNMAL KL K VYDPP+N+SR+ISFYYRENNR NYNER+N N
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALTKLKKNVYDPPSNTSRRISFYYRENNRGNYNERNNSN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           +DEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRF LFERTERR 
Sbjct: 181 SDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTERRG 240

Query: 241 AS--SNNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWG 282
           AS  SNNTVS AP  SLIDD+L+YLIRAMEDALMLRNLAGFWG
Sbjct: 241 ASSNSNNTVSTAPPSSLIDDDLMYLIRAMEDALMLRNLAGFWG 261

[Close]

BLAST of Lag0015118 vs. NCBI nr
Match: XP_008455285.1 (PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo])

HSP 1 Score: 423.7 bits (1088), Expect = 1.2e-114
Identity = 219/284 (77.11%), Postives = 237/284 (83.45%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRRSTNFPPGIWQARREYDRQRYNRN+SNR+NSTP + +SFQNS+REIDPVPW+PAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGE- 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
                                 MITRILDIG  PEEP AIVRIIT SRPRQ+EASP  AE
Sbjct: 61  ---------------------TMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           L+SPTPRS GNSTLT EEQNMALNKL KKVYDPP+N+SR+I+FYYRENNR NYNER N N
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTERR 240
           +DEEGK CAVCLEDFQPKEEVMTTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RTERR
Sbjct: 181 SDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERR 240

Query: 241 EASS--NNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWG 282
            ASS  NN VSIAP  SL+DDEL+YLIRAME+ALMLRN+A FWG
Sbjct: 241 GASSNFNNNVSIAPPSSLVDDELMYLIRAMEEALMLRNVAEFWG 262

[Close]

BLAST of Lag0015118 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.7e-11
Identity = 29/58 (50.00%), Postives = 36/58 (62.07%), Query Frame = 0


Query: 188 CAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERREASSNN 246
           CAVC E F+ + E    PC H+FH++CIVPW+     CPVCRF L     RR  S+NN
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR--SNNN 254

[Close]

BLAST of Lag0015118 vs. ExPASy Swiss-Prot
Match: Q9Y4L5 (E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 2.7e-11
Identity = 31/69 (44.93%), Postives = 42/69 (60.87%), Query Frame = 0


Query: 185 GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL-----FERTERR 244
           G  C VC ED+  +EEV   PCNH FH  CIVPW++ +  CPVCR +L       +++  
Sbjct: 225 GLECPVCKEDYTVEEEVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQSQST 284

Query: 245 EASSNNTVS 249
           EAS++N  S
Sbjct: 285 EASASNRFS 293

[Close]

BLAST of Lag0015118 vs. ExPASy Swiss-Prot
Match: Q9D0C1 (E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.7e-11
Identity = 31/69 (44.93%), Postives = 42/69 (60.87%), Query Frame = 0


Query: 185 GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL-----FERTERR 244
           G  C VC ED+  +E+V   PCNH FH  CIVPW++ +  CPVCR +L       +T+  
Sbjct: 226 GLECPVCKEDYTVEEKVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQTQSS 285

Query: 245 EASSNNTVS 249
           EAS++N  S
Sbjct: 286 EASASNRFS 294

[Close]

BLAST of Lag0015118 vs. ExPASy Swiss-Prot
Match: Q9C1X4 (Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP32A8.03c PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.0e-10
Identity = 26/67 (38.81%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 182 DEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERREA 241
           DEEG+ C +C+E F+  ++V+  PC H FHE CI PW++ NG C +CR  +   +++R  
Sbjct: 391 DEEGE-CTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICRAPVDPNSQQRNN 450

Query: 242 SSNNTVS 249
           +S ++ +
Sbjct: 451 TSTDSAN 456

[Close]

BLAST of Lag0015118 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.3e-10
Identity = 22/49 (44.90%), Postives = 36/49 (73.47%), Query Frame = 0


Query: 184 EGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL 233
           E  +CAVC+++F+   +V   PC H+FH++C++PW++ +  CPVCRF L
Sbjct: 212 EMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

[Close]

BLAST of Lag0015118 vs. ExPASy TrEMBL
Match: A0A6J1JPC8 (E3 ubiquitin-protein ligase EL5 OS=Cucurbita maxima OX=3661 GN=LOC111486293 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.3e-122
Identity = 219/281 (77.94%), Postives = 243/281 (86.48%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRRSTNFPPG+W+ARREYDRQRYN NDSNRM+ST P+P+ FQNS R+ +P+P +P   I
Sbjct: 1   MSRRSTNFPPGLWRARREYDRQRYNTNDSNRMSSTSPHPQPFQNSSRDTEPIPSRPTRGI 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
           DPVP +P  E+DP+PWRP  GAMI R+L+I    E+P AIVRIIT SRPRQ+EAS R AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           L+ PTPRS GNSTLT EEQNMALNKL KKVYDPPNN+SR+ISFYYRENNRRNYNER NG 
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERRNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWG 282
           ASSNNT SIA   +L+DDELVYLIRAMED+LMLRNL G WG
Sbjct: 241 ASSNNTASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

[Close]

BLAST of Lag0015118 vs. ExPASy TrEMBL
Match: A0A6J1ES11 (E3 ubiquitin-protein ligase EL5 OS=Cucurbita moschata OX=3662 GN=LOC111437204 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.0e-122
Identity = 218/281 (77.58%), Postives = 242/281 (86.12%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRR TNFPPG+W+ARREYD QRYN NDSNRM+ST P+P+ FQNS R+ +P+P +P G I
Sbjct: 1   MSRRPTNFPPGLWRARREYDHQRYNTNDSNRMSSTSPHPQPFQNSSRDTNPIPSRPTGGI 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
           DPVP +P  E+DP+PWRP  GAMI R+L+I    E+P AIVRIIT SRPRQ+EAS R AE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           L+ PTPRS GNSTLT EEQNMALNKL KKVYDPPNN+SR+ISFYYRENNRRNYNERSNG 
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERSNGE 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
           NDE+GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKS+GQCP CRF LFERT RRE
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 ASSNNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWG 282
           ASSNN  SIA   +L+DDELVYLIRAMED+LMLRNL G WG
Sbjct: 241 ASSNNAASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

[Close]

BLAST of Lag0015118 vs. ExPASy TrEMBL
Match: A0A1S3C054 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495488 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 6.0e-115
Identity = 219/284 (77.11%), Postives = 237/284 (83.45%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRRSTNFPPGIWQARREYDRQRYNRN+SNR+NSTP + +SFQNS+REIDPVPW+PAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGE- 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
                                 MITRILDIG  PEEP AIVRIIT SRPRQ+EASP  AE
Sbjct: 61  ---------------------TMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           L+SPTPRS GNSTLT EEQNMALNKL KKVYDPP+N+SR+I+FYYRENNR NYNER N N
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTERR 240
           +DEEGK CAVCLEDFQPKEEVMTTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RTERR
Sbjct: 181 SDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERR 240

Query: 241 EASS--NNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWG 282
            ASS  NN VSIAP  SL+DDEL+YLIRAME+ALMLRN+A FWG
Sbjct: 241 GASSNFNNNVSIAPPSSLVDDELMYLIRAMEEALMLRNVAEFWG 262

[Close]

BLAST of Lag0015118 vs. ExPASy TrEMBL
Match: A0A5D3C526 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003170 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 3.0e-114
Identity = 218/283 (77.03%), Postives = 237/283 (83.75%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRRSTNFPPGIWQARREYDRQRYNRN+SNR+NSTP + +SFQNS+REIDPVPW+PAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGE- 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
                                 MITRILDIG  PEEP AIVRIIT SRPRQ+EASP  AE
Sbjct: 61  ---------------------TMITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           L+SPTPRS GNSTLT EEQNMALNKL KKVYDPP+N+SR+I+FYYRENNR NYNER N N
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFE-RTERR 240
           +DEEGK CAVCLEDFQPKEEVMTTPCNHMFHE+CIVPWVKSNGQCPVCRF LFE RTERR
Sbjct: 181 SDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERR 240

Query: 241 EASS--NNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFW 281
            ASS  NN+VSIAP  SL+DDEL+YLIRAME+ALMLRN+A FW
Sbjct: 241 GASSNFNNSVSIAPPSSLVDDELMYLIRAMEEALMLRNVAEFW 261

[Close]

BLAST of Lag0015118 vs. ExPASy TrEMBL
Match: A0A0A0K1V1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G049220 PE=4 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.1e-113
Identity = 215/284 (75.70%), Postives = 236/284 (83.10%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRMNSTPPNPRSFQNSHREIDPVPWQPAGEI 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN+SNR NSTP + +SFQNS+R+IDPVPW+PAGE 
Sbjct: 1   MSRRSTNFPPGIWQARREHDRQRYNRNESNRTNSTPSHTQSFQNSYRQIDPVPWRPAGE- 60

Query: 61  DPVPWQPVGEIDPVPWRPAEGAMITRILDIGRPPEEPTAIVRIITQSRPRQLEASPRLAE 120
                                 +ITRILDIG  PEEP AIVRIIT SRPRQLEASP  AE
Sbjct: 61  ---------------------TVITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAE 120

Query: 121 LHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSSRKISFYYRENNRRNYNERSNGN 180
           L+SPTPRS GNSTLT EEQNMALNKL KKVYDPP+N+SR+ISFYYRENNR N NER N N
Sbjct: 121 LYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSN 180

Query: 181 NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERRE 240
            DEEGKRCAVCLEDFQPKEEVMTTPCNHMFHE+CIVPWVKS+GQCPVCRF LFERTERR 
Sbjct: 181 GDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRG 240

Query: 241 ASS---NNTVSIAPARSLIDDELVYLIRAMEDALMLRNLAGFWG 282
           ++S   NN+VSIAP  SL+D+ELVYLIRAME+ALMLRN+A FWG
Sbjct: 241 SASSNFNNSVSIAPPSSLVDEELVYLIRAMEEALMLRNVAEFWG 262

[Close]

BLAST of Lag0015118 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 2.6e-30
Identity = 73/182 (40.11%), Postives = 103/182 (56.59%), Query Frame = 0


Query: 101 VRIITQSRPRQLEASPRLAELHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSS-- 160
           +R     R + LEA  +     S  P S   S+    E++  L  L K+ Y+P   S+  
Sbjct: 53  IRNSNNLRRQHLEALRQAVHNQSTRPESSSRSSSQTNEEDEILKHLTKETYNPVPKSTLL 112

Query: 161 RKISFYYRENNRRNYNERSNGN--NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIV 220
           R +S YYR  N  + + R+  N   +E+ KRC+VCLEDF+PKE VM TPC HMFHEECIV
Sbjct: 113 RSLSLYYRNKNPGSESSRNTQNLSGEEDDKRCSVCLEDFEPKETVMLTPCKHMFHEECIV 172

Query: 221 PWVKSNGQCPVCRFALFERTERRE---ASSNNTVSIAPARSLIDDELVYLIRAMEDALML 276
           PW+K+ GQCPVCRF + + T++     A+ +N         L   EL+ ++RAME+  + 
Sbjct: 173 PWLKTKGQCPVCRFVILKPTKQDSSPPANGSNLTGDMTTNDLFTLELLSVVRAMEETFLF 232

[Close]

BLAST of Lag0015118 vs. TAIR 10
Match: AT3G58720.2 (RING/U-box superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 2.6e-30
Identity = 73/182 (40.11%), Postives = 103/182 (56.59%), Query Frame = 0


Query: 101 VRIITQSRPRQLEASPRLAELHSPTPRSRGNSTLTPEEQNMALNKLNKKVYDPPNNSS-- 160
           +R     R + LEA  +     S  P S   S+    E++  L  L K+ Y+P   S+  
Sbjct: 81  IRNSNNLRRQHLEALRQAVHNQSTRPESSSRSSSQTNEEDEILKHLTKETYNPVPKSTLL 140

Query: 161 RKISFYYRENNRRNYNERSNGN--NDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIV 220
           R +S YYR  N  + + R+  N   +E+ KRC+VCLEDF+PKE VM TPC HMFHEECIV
Sbjct: 141 RSLSLYYRNKNPGSESSRNTQNLSGEEDDKRCSVCLEDFEPKETVMLTPCKHMFHEECIV 200

Query: 221 PWVKSNGQCPVCRFALFERTERRE---ASSNNTVSIAPARSLIDDELVYLIRAMEDALML 276
           PW+K+ GQCPVCRF + + T++     A+ +N         L   EL+ ++RAME+  + 
Sbjct: 201 PWLKTKGQCPVCRFVILKPTKQDSSPPANGSNLTGDMTTNDLFTLELLSVVRAMEETFLF 260

[Close]

BLAST of Lag0015118 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.9e-12
Identity = 29/58 (50.00%), Postives = 36/58 (62.07%), Query Frame = 0


Query: 188 CAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFALFERTERREASSNN 246
           CAVC E F+ + E    PC H+FH++CIVPW+     CPVCRF L     RR  S+NN
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR--SNNN 254

[Close]

BLAST of Lag0015118 vs. TAIR 10
Match: AT2G44330.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.6e-11
Identity = 24/56 (42.86%), Postives = 36/56 (64.29%), Query Frame = 0


Query: 177 SNGNNDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL 233
           S+ ++D+    CA+C EDF   E     PCNH++H +CI+PW+ S+  CP+CR  L
Sbjct: 85  SSASSDDSALPCAICREDFVVGESARRLPCNHLYHNDCIIPWLTSHNSCPLCRVEL 140

[Close]

BLAST of Lag0015118 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.6e-11
Identity = 22/49 (44.90%), Postives = 36/49 (73.47%), Query Frame = 0


Query: 184 EGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFAL 233
           E  +CAVC+++F+   +V   PC H+FH++C++PW++ +  CPVCRF L
Sbjct: 212 EMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

The following BLAST results are available for this feature:

BLAST of Lag0015118 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023529368.1	1.2e-122	77.58	E3 ubiquitin-protein ligase EL5 [Cucurbita pepo subsp. pepo]	[more]
XP_022989128.1	2.8e-122	77.94	E3 ubiquitin-protein ligase EL5 [Cucurbita maxima]	[more]
XP_022930836.1	6.1e-122	77.58	E3 ubiquitin-protein ligase EL5 [Cucurbita moschata] >KAG7022947.1 E3 ubiquitin-...	[more]
XP_038888228.1	8.3e-119	79.15	uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida]	[more]
XP_008455285.1	1.2e-114	77.11	PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo]	[more]

BLAST of Lag0015118 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940T5	2.7e-11	50.00	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q9Y4L5	2.7e-11	44.93	E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2	[more]
Q9D0C1	2.7e-11	44.93	E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1	[more]
Q9C1X4	1.0e-10	38.81	Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (stra...	[more]
Q8LPN7	2.3e-10	44.90	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]

BLAST of Lag0015118 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JPC8	1.3e-122	77.94	E3 ubiquitin-protein ligase EL5 OS=Cucurbita maxima OX=3661 GN=LOC111486293 PE=4...	[more]
A0A6J1ES11	3.0e-122	77.58	E3 ubiquitin-protein ligase EL5 OS=Cucurbita moschata OX=3662 GN=LOC111437204 PE...	[more]
A0A1S3C054	6.0e-115	77.11	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A5D3C526	3.0e-114	77.03	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A0A0K1V1	1.1e-113	75.70	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G049220 P...	[more]

BLAST of Lag0015118 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58720.1	2.6e-30	40.11	RING/U-box superfamily protein	[more]
AT3G58720.2	2.6e-30	40.11	RING/U-box superfamily protein	[more]
AT5G59550.1	1.9e-12	50.00	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G44330.1	1.6e-11	42.86	RING/U-box superfamily protein	[more]
AT3G19950.1	1.6e-11	44.90	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 188..228 e-value: 1.8E-9 score: 47.4
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 187..229 e-value: 3.6E-14 score: 52.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 188..229 score: 13.536741
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 172..248 e-value: 1.2E-21 score: 77.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 110..137
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..62
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..45
None	No IPR available	PANTHER	PTHR22765:SF328	ANAPHASE-PROMOTING COMPLEX SUBUNIT 11 RING-H2 FINGER PROTEIN	coord: 139..275
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 139..275
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 188..229 e-value: 1.31473E-19 score: 78.1125
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 182..238

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0015118.1	Lag0015118.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
molecular_function	GO:0061630	ubiquitin protein ligase activity