Lag0015081 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0015081
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr12: 7373055 .. 7375255 (+)
RNA-Seq ExpressionLag0015081
SyntenyLag0015081
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAAGCTGAAGCTCCATTGCTCTCAGCCATGGCGTTGTTGCTGCAATTCCAAGCTATTTCAGGCTCTACTCTACTCAACTAAAGGCTTCCCTTCTTCTCGAAGTCCGGAGGATTCTCTCTACCGAAGGGTTTCTCAGGCAGGCGATCCTCGAATCTCCATTGTTCGCGTGCTGGACCAGTGGGTCGAAGAAGGCCGAGAAGTCAAGCAATCTGACCTCCAAAAGCTCATCAAGCAGCTCAGGAAGTTCCGTCGCTTCAACCATGCTCTGCAGGTATCACTTTTTTCTGAAATTGCATTCATTGTGCATTACATGAAAATTGTTTACATCTGCTCTGGCCCTGTCCTATGATAATGAATGTTGAGTTGGGAAGCGGGTTACGTCTAAAAATTCATTTAGTGGTATGGAATTGTTGTCTTTTACAGAGACGGCTTTGCATTATGGATTAATTGGAAACAGAAATTTAGGTTGGATATGATACGAAATGAAATTATTGGTACTATTAGACTAGTTTGGATATCGTAATGAATTCCATTTCAGTGCAACTTGGAAGTGGAAGCTAAGAGTGACCAACTCTTTCGGTCCAGCAATAAGAATTAACTTTGCAATTAAAAAACAATGGTATGCATCTTTATTGAGGACTACTAAACTATCAGAACAGTCAGTGTTTCTGTTCTGCGAGCTTTTAGTTTTGGCCATTTTGTATGTTTAACCTTCCCCAGCTTGCTTTTGCCCACAATTAAGGTCAGGACACTTACATCTTCAGTCACCATCTTCATTATCTTGCCTGGTTAGGTCAACTAATTGTAGCCATATTTCCCTTTTTGCTTGTCTTCTTCACTCAGTCTCAAACCAAAGCATTGAAATTGGATCATCATAAATACGTCTTTGTTATTCACAATTTGACATTATTGATTACTGTTTTGCAACAGTTGTGTGAATGGATAAGTAATGAAAGGAACCATGACCCGTCGCCTGGGGACATTGCTATTCAGTTGCACTTAATTTCAAAAGTTCATGGCTTAGAACAAGCTGAGAAGTATTTTAGCAGTATCATGGAATCTTCAAGAGATTATAAGGTCTATGGAGCACTTCTAAACTGTTATGTAGAGGATAAAAATTTGGAGAAGGCAGAAGCACTCATGGAGAAGATGAGGGAAGTAGGATTTATGAAAACACCACTACCCTATAATGTTATGTTAAACCTTTATGCTCATCTGGGTAAACATGAGAAACTTGATGAATTAATGGAAGAGATGGAAGAGATGGGAATTGCTCATGATAGATTTACCTATAACATTCGTATGAATGCTTATGCAGCTACTTCCAATATAACAAATATGGAAAAGCTTTTGTTGAAGATGGAGGCAGATCCACTAATTACTATGGATTGGCATGCGTATTTCGTTGTAGCAAATGGATACTTCAAAGTTGGTCTTTCTGAAAAGAGTATAATGATGCTGAAGAGATCAGAGCAACTCATTGGTGATAAGCAAAAGTGGTTTGCATATGAATGTCTCATTACACTGTATGCTGCTATCGGTAATAAGGATGAGGTGTATCGAGTTTGGAACTTGTACACTAATCTGAAACGAAGATTCAATACAGGATATCTTTGTATAATAAGTTCATTAATGAAACTGGATGATATTGACGGTGCTGAAAAAATCTTGAAGGAGTGGGAATCAGGGGATACATGTTTTGACTTTAAAATTCCAAACATGATGATAAACAGTTATTGTAAGAAGGGACTTGTGGATAAGGCAGAAGAATATATAAGCAGGCTTATGGAGAATGGCAAGGAACCACAAGCAAGCACTTGGGATCGACTAGCAACTGGATATCATGCCAATGGCCAGACGATGAAGGCAGTAGAAACTATGAAGAAAGCAATTTCAGCTAGTCAACCTGTATGGAAGCCTAATTATTATACCTTGGCCGCATGTCTTGAATATTTGAAAACAAATGGAAATGTGGAGGTAGCAGAGGAAATCATAGCGCTCCTTCAAAAACACAACAATGTTTCCAAAAATATTTGCAATAGATTAGTAGATTATATCCACAGTGAAAACGAAACCTCAAATGCCCTTGATCAACTCGGCCTAGATGGTCAGATTGAGAGACTCGACCACCCTTCAGATCAAAACAAGCTTGACTTAGCTGAGCTAAAGTATGAAGAGACTTCTGATAGTAAGTGA

mRNA sequence

ATGATGAAGCTGAAGCTCCATTGCTCTCAGCCATGGCGTTGTTGCTGCAATTCCAAGCTATTTCAGGCTCTACTCTACTCAACTAAAGGCTTCCCTTCTTCTCGAAGTCCGGAGGATTCTCTCTACCGAAGGGTTTCTCAGGCAGGCGATCCTCGAATCTCCATTGTTCGCGTGCTGGACCAGTGGGTCGAAGAAGGCCGAGAAGTCAAGCAATCTGACCTCCAAAAGCTCATCAAGCAGCTCAGGAAGTTCCGTCGCTTCAACCATGCTCTGCAGTTGTGTGAATGGATAAGTAATGAAAGGAACCATGACCCGTCGCCTGGGGACATTGCTATTCAGTTGCACTTAATTTCAAAAGTTCATGGCTTAGAACAAGCTGAGAAGTATTTTAGCAGTATCATGGAATCTTCAAGAGATTATAAGGTCTATGGAGCACTTCTAAACTGTTATGTAGAGGATAAAAATTTGGAGAAGGCAGAAGCACTCATGGAGAAGATGAGGGAAGTAGGATTTATGAAAACACCACTACCCTATAATGTTATGTTAAACCTTTATGCTCATCTGGGTAAACATGAGAAACTTGATGAATTAATGGAAGAGATGGAAGAGATGGGAATTGCTCATGATAGATTTACCTATAACATTCGTATGAATGCTTATGCAGCTACTTCCAATATAACAAATATGGAAAAGCTTTTGTTGAAGATGGAGGCAGATCCACTAATTACTATGGATTGGCATGCGTATTTCGTTGTAGCAAATGGATACTTCAAAGTTGGTCTTTCTGAAAAGAGTATAATGATGCTGAAGAGATCAGAGCAACTCATTGGTGATAAGCAAAAGTGGTTTGCATATGAATGTCTCATTACACTGTATGCTGCTATCGGTAATAAGGATGAGGTGTATCGAGTTTGGAACTTGTACACTAATCTGAAACGAAGATTCAATACAGGATATCTTTGTATAATAAGTTCATTAATGAAACTGGATGATATTGACGGTGCTGAAAAAATCTTGAAGGAGTGGGAATCAGGGGATACATGTTTTGACTTTAAAATTCCAAACATGATGATAAACAGTTATTGTAAGAAGGGACTTGTGGATAAGGCAGAAGAATATATAAGCAGGCTTATGGAGAATGGCAAGGAACCACAAGCAAGCACTTGGGATCGACTAGCAACTGGATATCATGCCAATGGCCAGACGATGAAGGCAGTAGAAACTATGAAGAAAGCAATTTCAGCTAGTCAACCTGTATGGAAGCCTAATTATTATACCTTGGCCGCATGTCTTGAATATTTGAAAACAAATGGAAATGTGGAGGTAGCAGAGGAAATCATAGCGCTCCTTCAAAAACACAACAATGTTTCCAAAAATATTTGCAATAGATTAGTAGATTATATCCACAGTGAAAACGAAACCTCAAATGCCCTTGATCAACTCGGCCTAGATGGTCAGATTGAGAGACTCGACCACCCTTCAGATCAAAACAAGCTTGACTTAGCTGAGCTAAAGTATGAAGAGACTTCTGATAGTAAGTGA

Coding sequence (CDS)

ATGATGAAGCTGAAGCTCCATTGCTCTCAGCCATGGCGTTGTTGCTGCAATTCCAAGCTATTTCAGGCTCTACTCTACTCAACTAAAGGCTTCCCTTCTTCTCGAAGTCCGGAGGATTCTCTCTACCGAAGGGTTTCTCAGGCAGGCGATCCTCGAATCTCCATTGTTCGCGTGCTGGACCAGTGGGTCGAAGAAGGCCGAGAAGTCAAGCAATCTGACCTCCAAAAGCTCATCAAGCAGCTCAGGAAGTTCCGTCGCTTCAACCATGCTCTGCAGTTGTGTGAATGGATAAGTAATGAAAGGAACCATGACCCGTCGCCTGGGGACATTGCTATTCAGTTGCACTTAATTTCAAAAGTTCATGGCTTAGAACAAGCTGAGAAGTATTTTAGCAGTATCATGGAATCTTCAAGAGATTATAAGGTCTATGGAGCACTTCTAAACTGTTATGTAGAGGATAAAAATTTGGAGAAGGCAGAAGCACTCATGGAGAAGATGAGGGAAGTAGGATTTATGAAAACACCACTACCCTATAATGTTATGTTAAACCTTTATGCTCATCTGGGTAAACATGAGAAACTTGATGAATTAATGGAAGAGATGGAAGAGATGGGAATTGCTCATGATAGATTTACCTATAACATTCGTATGAATGCTTATGCAGCTACTTCCAATATAACAAATATGGAAAAGCTTTTGTTGAAGATGGAGGCAGATCCACTAATTACTATGGATTGGCATGCGTATTTCGTTGTAGCAAATGGATACTTCAAAGTTGGTCTTTCTGAAAAGAGTATAATGATGCTGAAGAGATCAGAGCAACTCATTGGTGATAAGCAAAAGTGGTTTGCATATGAATGTCTCATTACACTGTATGCTGCTATCGGTAATAAGGATGAGGTGTATCGAGTTTGGAACTTGTACACTAATCTGAAACGAAGATTCAATACAGGATATCTTTGTATAATAAGTTCATTAATGAAACTGGATGATATTGACGGTGCTGAAAAAATCTTGAAGGAGTGGGAATCAGGGGATACATGTTTTGACTTTAAAATTCCAAACATGATGATAAACAGTTATTGTAAGAAGGGACTTGTGGATAAGGCAGAAGAATATATAAGCAGGCTTATGGAGAATGGCAAGGAACCACAAGCAAGCACTTGGGATCGACTAGCAACTGGATATCATGCCAATGGCCAGACGATGAAGGCAGTAGAAACTATGAAGAAAGCAATTTCAGCTAGTCAACCTGTATGGAAGCCTAATTATTATACCTTGGCCGCATGTCTTGAATATTTGAAAACAAATGGAAATGTGGAGGTAGCAGAGGAAATCATAGCGCTCCTTCAAAAACACAACAATGTTTCCAAAAATATTTGCAATAGATTAGTAGATTATATCCACAGTGAAAACGAAACCTCAAATGCCCTTGATCAACTCGGCCTAGATGGTCAGATTGAGAGACTCGACCACCCTTCAGATCAAAACAAGCTTGACTTAGCTGAGCTAAAGTATGAAGAGACTTCTGATAGTAAGTGA

Protein sequence

MMKLKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYRVWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPNYYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETSNALDQLGLDGQIERLDHPSDQNKLDLAELKYEETSDSK
Homology
BLAST of Lag0015081 vs. NCBI nr
Match: XP_022147816.1 (pentatricopeptide repeat-containing protein At2g20710, mitochondrial [Momordica charantia])

HSP 1 Score: 899.8 bits (2324), Expect = 1.1e-257
Identity = 444/509 (87.23%), Postives = 476/509 (93.52%), Query Frame = 0

Query: 4   LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
           +KLHCSQPWR CC SK F+AL YSTK   SS SPEDSLYRRVSQAGDPRISI RVLDQWV
Sbjct: 2   MKLHCSQPWRGCCTSKAFRALFYSTKALTSSPSPEDSLYRRVSQAGDPRISIRRVLDQWV 61

Query: 64  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
           EEGR VK SDLQKLIKQLRKFRRFNHALQLCEWISNE NHDPSPGDIAI+LHLISKV+GL
Sbjct: 62  EEGRLVKISDLQKLIKQLRKFRRFNHALQLCEWISNEMNHDPSPGDIAIRLHLISKVYGL 121

Query: 124 EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
           EQAEKYFSSI ESSRDY+VYGALLNCYVED++LEKAE +M+KMRE+GFMKTPL +NVML+
Sbjct: 122 EQAEKYFSSINESSRDYRVYGALLNCYVEDRDLEKAEEIMQKMRELGFMKTPLSFNVMLS 181

Query: 184 LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
           LYAHLGKHEKLD LMEEMEEMGIAHDRFTYNIRMNA+AATSNITNMEKLLLKMEAD LIT
Sbjct: 182 LYAHLGKHEKLDALMEEMEEMGIAHDRFTYNIRMNAHAATSNITNMEKLLLKMEADRLIT 241

Query: 244 MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
           MDWHAY+VVANGYFK GLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNK EVYR
Sbjct: 242 MDWHAYYVVANGYFKAGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKAEVYR 301

Query: 304 VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
           VWNLYTNLKRR+NT YLCIISSLMKLDDI+GAEKILKEWESGDTCFDFKIPNMMIN YC+
Sbjct: 302 VWNLYTNLKRRYNTAYLCIISSLMKLDDIEGAEKILKEWESGDTCFDFKIPNMMINIYCR 361

Query: 364 KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
           KGLVDKAE YISRLME+GKEPQA+TWDRLATGYHANGQTMKAVET+KKAISASQP WKPN
Sbjct: 362 KGLVDKAEAYISRLMESGKEPQANTWDRLATGYHANGQTMKAVETIKKAISASQPGWKPN 421

Query: 424 YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETSNALDQLGL 483
            +TLAACLE+LKTN NVEVAEEII LL+KH+ VS  IC+ LVDY+HSE +TS+ALDQLGL
Sbjct: 422 DHTLAACLEFLKTNENVEVAEEIIRLLRKHDIVSIRICDGLVDYVHSEIQTSSALDQLGL 481

Query: 484 DGQIERLDHPSDQNKLDLAELKYEETSDS 513
           DGQIER +H SD+NKLD+AE+KYEETSDS
Sbjct: 482 DGQIERHNHASDRNKLDIAEVKYEETSDS 510

BLAST of Lag0015081 vs. NCBI nr
Match: KAG7022915.1 (Pentatricopeptide repeat-containing protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 892.5 bits (2305), Expect = 1.7e-255
Identity = 436/511 (85.32%), Postives = 469/511 (91.78%), Query Frame = 0

Query: 4   LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
           +KLH  QPWRC CNS++ + L YST G  S RSPEDSLY RVSQAGDPRISIV VLDQWV
Sbjct: 2   MKLHSLQPWRCYCNSRILRPLFYSTLGSTSFRSPEDSLYLRVSQAGDPRISIVSVLDQWV 61

Query: 64  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
           EEGREVKQSDLQKLIKQLR+FRRFNHALQLCEWISNERN +PSPGDIAIQLHLISKVHGL
Sbjct: 62  EEGREVKQSDLQKLIKQLRRFRRFNHALQLCEWISNERNQEPSPGDIAIQLHLISKVHGL 121

Query: 124 EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
           EQAEKYF+SI ESSRDYKVYGALLNCYVEDK LEK+EALMEKMRE+GF+KTPLPYN ML 
Sbjct: 122 EQAEKYFNSIRESSRDYKVYGALLNCYVEDKKLEKSEALMEKMRELGFLKTPLPYNTMLG 181

Query: 184 LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
           LYAHLGKHEKLDEL+EEMEEMGIA DRFTYNIRMNAYAA S++TNMEKLLLKMEADPLI+
Sbjct: 182 LYAHLGKHEKLDELIEEMEEMGIARDRFTYNIRMNAYAANSDVTNMEKLLLKMEADPLIS 241

Query: 244 MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
           MDWHAYFVVANGYFK GLS+KS++MLKRSEQLIGDKQ+WFAYECLITLYAAIGNKD+VYR
Sbjct: 242 MDWHAYFVVANGYFKAGLSDKSLLMLKRSEQLIGDKQRWFAYECLITLYAAIGNKDQVYR 301

Query: 304 VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
           VWNLY NLKRR+NTGYLCIISSLMKLDDI+GAE+ILKEWESGDTCFDFKIPNMM+NSYCK
Sbjct: 302 VWNLYANLKRRYNTGYLCIISSLMKLDDIEGAEEILKEWESGDTCFDFKIPNMMVNSYCK 361

Query: 364 KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
           KGLVDKAE YI RLMENGKEPQA+TWDRLATGYHAN QTMKAVE MKKAISAS P WKPN
Sbjct: 362 KGLVDKAEAYIGRLMENGKEPQANTWDRLATGYHANDQTMKAVEAMKKAISASPPGWKPN 421

Query: 424 YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETSNALDQLGL 483
           YYTLAACLEYLKTNGNVE AEEI+ LL KHN +S +I +RLVDY HS+N+TS+ALDQ GL
Sbjct: 422 YYTLAACLEYLKTNGNVEAAEEIVRLLGKHNIISSHINDRLVDYTHSDNQTSSALDQFGL 481

Query: 484 DGQIERLDHPSDQNKLDLAE-LKYEETSDSK 514
           DG IER+DH SD NKL+ AE LK EETSD K
Sbjct: 482 DGHIERIDHASDLNKLNFAEVLKNEETSDMK 512

BLAST of Lag0015081 vs. NCBI nr
Match: XP_022988744.1 (pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 891.7 bits (2303), Expect = 2.9e-255
Identity = 436/508 (85.83%), Postives = 467/508 (91.93%), Query Frame = 0

Query: 4   LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
           +KLH  QPWRC CNS++ + L YST G  S RSPEDSLYRRVSQAGDPRISIV VLDQWV
Sbjct: 2   MKLHSLQPWRCYCNSRILRPLFYSTLGSTSFRSPEDSLYRRVSQAGDPRISIVSVLDQWV 61

Query: 64  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
           EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERN +PSPGDIAIQLHLISKVHGL
Sbjct: 62  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNQEPSPGDIAIQLHLISKVHGL 121

Query: 124 EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
           EQAEKYF+SI ESSRDYKVYGALLNCYVEDK LEKAEALMEKMRE+GF+KTPLPYN ML 
Sbjct: 122 EQAEKYFNSIRESSRDYKVYGALLNCYVEDKKLEKAEALMEKMRELGFVKTPLPYNTMLG 181

Query: 184 LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
           LYAHLGKHEKLDEL+EEMEEMGIA DRFTYNIRMNAYAA S++TNMEKLLLKMEADPLI+
Sbjct: 182 LYAHLGKHEKLDELIEEMEEMGIARDRFTYNIRMNAYAANSDVTNMEKLLLKMEADPLIS 241

Query: 244 MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
           MDWHAYFVVANGYFK GLS+KS++MLKRSEQLIGDKQ+WFAYECLITLYAAIGNKD+VYR
Sbjct: 242 MDWHAYFVVANGYFKAGLSDKSLLMLKRSEQLIGDKQRWFAYECLITLYAAIGNKDQVYR 301

Query: 304 VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
           VWNLY NLKRR+NTGYLCIISSLMKLDDI+GAE+ILKEWESGDTCFDFKIPNMM+NSYCK
Sbjct: 302 VWNLYANLKRRYNTGYLCIISSLMKLDDIEGAEEILKEWESGDTCFDFKIPNMMVNSYCK 361

Query: 364 KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
           KGLVDKAE YISRLMENGKEPQA+TWDRLATGYHAN QTMKAVE MKKAISAS P WKPN
Sbjct: 362 KGLVDKAEAYISRLMENGKEPQANTWDRLATGYHANDQTMKAVEAMKKAISASPPGWKPN 421

Query: 424 YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETSNALDQLGL 483
           YYTLAACLEYLKTNGNVE  EEI+ LL KHN +S +I +R VDYIHS+N+TS+ALDQ GL
Sbjct: 422 YYTLAACLEYLKTNGNVEAVEEIVRLLGKHNIISSHINDRFVDYIHSDNQTSSALDQFGL 481

Query: 484 DGQIERLDHPSDQNKLDLAE-LKYEETS 511
           D  IER+DH SD NKL+ AE LK EETS
Sbjct: 482 DDHIERIDHASDLNKLNFAEVLKNEETS 509

BLAST of Lag0015081 vs. NCBI nr
Match: XP_023531849.1 (pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891.7 bits (2303), Expect = 2.9e-255
Identity = 435/511 (85.13%), Postives = 468/511 (91.59%), Query Frame = 0

Query: 4   LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
           +KLH  QPWRC CNS++ + L YST G  S RSPEDSLY RVSQAGDPRISIV VLDQWV
Sbjct: 2   MKLHSLQPWRCYCNSRILRPLFYSTSGSTSFRSPEDSLYLRVSQAGDPRISIVSVLDQWV 61

Query: 64  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
           EEGREVKQSDLQKLIKQLR+FRRFNHALQLCEWISNERN +PSPGDIAIQLHLISKVHGL
Sbjct: 62  EEGREVKQSDLQKLIKQLRRFRRFNHALQLCEWISNERNQEPSPGDIAIQLHLISKVHGL 121

Query: 124 EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
           EQAEKYF+SI ESSRDYKVYGALLNCYVEDKNLEKAEALMEKMRE+GF+KTPLPYN M  
Sbjct: 122 EQAEKYFNSIRESSRDYKVYGALLNCYVEDKNLEKAEALMEKMRELGFLKTPLPYNTMFG 181

Query: 184 LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
           LYAHLGKHEKLDEL+EEMEEMGIA DRFTYNIRMNAYAA S++TNMEKLLLKMEADPLI+
Sbjct: 182 LYAHLGKHEKLDELIEEMEEMGIARDRFTYNIRMNAYAANSDVTNMEKLLLKMEADPLIS 241

Query: 244 MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
           MDWH YFVVANGYFK GLS+KS++MLKRSE LIGDKQ+WFAYECLITLYAAIGNKD+VYR
Sbjct: 242 MDWHGYFVVANGYFKAGLSDKSLLMLKRSEHLIGDKQRWFAYECLITLYAAIGNKDQVYR 301

Query: 304 VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
           VWNLY NLKRR+NTGYLCIISSLMKLDDI+GAE+ILKEWE GDTCFDFKIPNMM+NSYCK
Sbjct: 302 VWNLYANLKRRYNTGYLCIISSLMKLDDIEGAEEILKEWELGDTCFDFKIPNMMVNSYCK 361

Query: 364 KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
           KGLVDKAE YI+RLMENGKEPQA+TWDRLATGYHANGQTMKAVE MKKAISAS P WKPN
Sbjct: 362 KGLVDKAEAYINRLMENGKEPQANTWDRLATGYHANGQTMKAVEAMKKAISASPPGWKPN 421

Query: 424 YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETSNALDQLGL 483
           YYTLAACLEYLKTNGNVE AEEI+ LL KHN +S +I +RLVDYIHS+N+TS+AL Q GL
Sbjct: 422 YYTLAACLEYLKTNGNVEAAEEIVRLLGKHNIISSHINDRLVDYIHSDNQTSSALHQFGL 481

Query: 484 DGQIERLDHPSDQNKLDLAE-LKYEETSDSK 514
           DG IER+DH SD NKL+ AE LK EETSD K
Sbjct: 482 DGHIERIDHASDLNKLNFAEVLKNEETSDMK 512

BLAST of Lag0015081 vs. NCBI nr
Match: KAG6589215.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 889.4 bits (2297), Expect = 1.5e-254
Identity = 435/511 (85.13%), Postives = 469/511 (91.78%), Query Frame = 0

Query: 4   LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
           +KLH  QPWRC CNS++ + L YST G  S RSPEDSLY RVSQAGDPRISIV VLDQWV
Sbjct: 2   MKLHSLQPWRCYCNSRILRPLFYSTLGSTSFRSPEDSLYLRVSQAGDPRISIVSVLDQWV 61

Query: 64  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
           EEGREVKQSDLQKLIKQLR+FRRFNHALQLCEWISNERN +PSPGDIAIQLHLISKVHGL
Sbjct: 62  EEGREVKQSDLQKLIKQLRRFRRFNHALQLCEWISNERNQEPSPGDIAIQLHLISKVHGL 121

Query: 124 EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
           EQAEKYF+SI ESSRDYKVYGALLNCYVEDK LEK+EALMEKMRE+GF+KTPLPYN ML 
Sbjct: 122 EQAEKYFNSIRESSRDYKVYGALLNCYVEDKKLEKSEALMEKMRELGFLKTPLPYNTMLG 181

Query: 184 LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
           LYAHLGKHEKLDEL+EEMEEMGIA DRFTYNIRMNAYAA S++TNMEKLLLKMEADPLI+
Sbjct: 182 LYAHLGKHEKLDELIEEMEEMGIARDRFTYNIRMNAYAANSDVTNMEKLLLKMEADPLIS 241

Query: 244 MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
           MDWHAYFVVANGYFK GLS+KS++MLKRSEQLIGDKQ+WFAYECLITLYAAIGNKD+VYR
Sbjct: 242 MDWHAYFVVANGYFKAGLSDKSLLMLKRSEQLIGDKQRWFAYECLITLYAAIGNKDQVYR 301

Query: 304 VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
           VWNLY NLKRR+NTGYLCIISSLMKLDDI+GAE+ILKEWESGDTCFDFKIPNMM+NSYCK
Sbjct: 302 VWNLYANLKRRYNTGYLCIISSLMKLDDIEGAEEILKEWESGDTCFDFKIPNMMVNSYCK 361

Query: 364 KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
           KGLVDKAE YI RLME+GKEPQA+TWDRLATGYHAN QTMKAVE MKKAISAS P WKPN
Sbjct: 362 KGLVDKAEAYIGRLMEDGKEPQANTWDRLATGYHANDQTMKAVEAMKKAISASPPGWKPN 421

Query: 424 YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETSNALDQLGL 483
           YYTLAACLEYLKTNGNVEVAEEI+ LL KHN +S +I  RLVDY +S+N+TS+ALDQ GL
Sbjct: 422 YYTLAACLEYLKTNGNVEVAEEIVRLLGKHNIISSHINERLVDYTYSDNQTSSALDQFGL 481

Query: 484 DGQIERLDHPSDQNKLDLAE-LKYEETSDSK 514
           DG IER+DH SD NKL+ AE LK EETSD K
Sbjct: 482 DGHIERIDHASDLNKLNFAEVLKNEETSDMK 512

BLAST of Lag0015081 vs. ExPASy Swiss-Prot
Match: Q9SKU6 (Pentatricopeptide repeat-containing protein At2g20710, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g20710 PE=2 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.6e-121
Identity = 204/434 (47.00%), Postives = 306/434 (70.51%), Query Frame = 0

Query: 24  LLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLRK 83
           L +S K  PS   P D+L RRV+++GDP  SI++VLD W+++G  VK S+L  +IK LRK
Sbjct: 23  LFHSGKTTPSPLDPYDTLQRRVARSGDPSASIIKVLDGWLDQGNLVKTSELHSIIKMLRK 82

Query: 84  FRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKVY 143
           F RF+HALQ+ +W+S  R H+ S GD+AI+L LI+KV GL +AEK+F +I    R+Y +Y
Sbjct: 83  FSRFSHALQISDWMSEHRVHEISEGDVAIRLDLIAKVGGLGEAEKFFETIPMERRNYHLY 142

Query: 144 GALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEMEE 203
           GALLNCY   K L KAE + ++M+E+GF+K  LPYNVMLNLY   GK+  +++L+ EME+
Sbjct: 143 GALLNCYASKKVLHKAEQVFQEMKELGFLKGCLPYNVMLNLYVRTGKYTMVEKLLREMED 202

Query: 204 MGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLSE 263
             +  D FT N R++AY+  S++  MEK L++ EAD  + +DW  Y   ANGY K GL+E
Sbjct: 203 ETVKPDIFTVNTRLHAYSVVSDVEGMEKFLMRCEADQGLHLDWRTYADTANGYIKAGLTE 262

Query: 264 KSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYRVWNLYTNLKRRFNTGYLCII 323
           K++ ML++SEQ++  +++  AYE L++ Y A G K+EVYR+W+LY  L   +NTGY+ +I
Sbjct: 263 KALEMLRKSEQMVNAQKRKHAYEVLMSFYGAAGKKEEVYRLWSLYKELDGFYNTGYISVI 322

Query: 324 SSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENGKE 383
           S+L+K+DDI+  EKI++EWE+G + FD +IP+++I  YCKKG+++KAEE ++ L++  + 
Sbjct: 323 SALLKMDDIEEVEKIMEEWEAGHSLFDIRIPHLLITGYCKKGMMEKAEEVVNILVQKWRV 382

Query: 384 PQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPNYYTLAACLEYLKTNGNVEVA 443
              STW+RLA GY   G+  KAVE  K+AI  S+P W+P+   L +C++YL+   ++E  
Sbjct: 383 EDTSTWERLALGYKMAGKMEKAVEKWKRAIEVSKPGWRPHQVVLMSCVDYLEGQRDMEGL 442

Query: 444 EEIIALLQKHNNVS 458
            +I+ LL +  ++S
Sbjct: 443 RKILRLLSERGHIS 456

BLAST of Lag0015081 vs. ExPASy Swiss-Prot
Match: Q84JR3 (Pentatricopeptide repeat-containing protein At4g21705, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g21705 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.3e-80
Identity = 158/446 (35.43%), Postives = 266/446 (59.64%), Query Frame = 0

Query: 23  ALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLR 82
           A L +++ + ++R  + +LY ++S  GDP+ S+   L  WV+ G++V  ++L +++  LR
Sbjct: 9   ANLIASRYYYTNRVKKTTLYSKISPLGDPKSSVYPELQNWVQCGKKVSVAELIRIVHDLR 68

Query: 83  KFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKV 142
           + +RF HAL++ +W++       SP + A+ L LI +V+G   AE+YF ++ E  ++ K 
Sbjct: 69  RRKRFLHALEVSKWMNETGVCVFSPTEHAVHLDLIGRVYGFVTAEEYFENLKEQYKNDKT 128

Query: 143 YGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEME 202
           YGALLNCYV  +N+EK+    EKM+E+GF+ + L YN ++ LY ++G+HEK+ +++EEM+
Sbjct: 129 YGALLNCYVRQQNVEKSLLHFEKMKEMGFVTSSLTYNNIMCLYTNIGQHEKVPKVLEEMK 188

Query: 203 EMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLS 262
           E  +A D ++Y I +NA+ A  ++  +   L  ME    ITMDW+ Y V A  Y   G  
Sbjct: 189 EENVAPDNYSYRICINAFGAMYDLERIGGTLRDMERRQDITMDWNTYAVAAKFYIDGGDC 248

Query: 263 EKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYRVWNLYTNL-KRRFNTGYLC 322
           ++++ +LK SE  + +K+    Y  LITLYA +G K EV R+W+L  ++ KRR N  YL 
Sbjct: 249 DRAVELLKMSENRL-EKKDGEGYNHLITLYARLGKKIEVLRLWDLEKDVCKRRINQDYLT 308

Query: 323 IISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENG 382
           ++ SL+K+D +  AE++L EW+S   C+DF++PN +I  Y  K + +KAE  +  L   G
Sbjct: 309 VLQSLVKIDALVEAEEVLTEWKSSGNCYDFRVPNTVIRGYIGKSMEEKAEAMLEDLARRG 368

Query: 383 KEPQASTWDRLATGYHANGQTMKAVETMKKA--ISASQPVWKPNYYTLAACLEYLKTNGN 442
           K     +W+ +AT Y   G    A + MK A  +      W+P    + + L ++   G+
Sbjct: 369 KATTPESWELVATAYAEKGTLENAFKCMKTALGVEVGSRKWRPGLTLVTSVLSWVGDEGS 428

Query: 443 VEVAEEIIALLQKHNNVSKNICNRLV 466
           ++  E  +A L+    V+K + + LV
Sbjct: 429 LKEVESFVASLRNCIGVNKQMYHALV 453

BLAST of Lag0015081 vs. ExPASy Swiss-Prot
Match: Q8LPS6 (Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX=3702 GN=At1g02150 PE=2 SV=2)

HSP 1 Score: 284.6 bits (727), Expect = 2.1e-75
Identity = 148/455 (32.53%), Postives = 271/455 (59.56%), Query Frame = 0

Query: 39  DSLYRRVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWIS 98
           +++Y+++S    P +    VL+QW + GR++ + +L +++K+LRK++R N AL++ +W++
Sbjct: 67  NAIYKKISLMEKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKRANQALEVYDWMN 126

Query: 99  NE-RNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLE 158
           N       S  D AIQL LI KV G+  AE++F  + E+ +D +VYG+LLN YV  K+ E
Sbjct: 127 NRGERFRLSASDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKSRE 186

Query: 159 KAEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRM 218
           KAEAL+  MR+ G+   PLP+NVM+ LY +L +++K+D ++ EM++  I  D ++YNI +
Sbjct: 187 KAEALLNTMRDKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNIWL 246

Query: 219 NAYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIG 278
           ++  +  ++  ME +  +M++D  I  +W  +  +A  Y K+G +EK+   L++ E  I 
Sbjct: 247 SSCGSLGSVEKMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEARIT 306

Query: 279 DKQKWFAYECLITLYAAIGNKDEVYRVWNLYTNLKRRF-NTGYLCIISSLMKLDDIDGAE 338
            + +   Y  L++LY ++GNK E+YRVW++Y ++     N GY  ++SSL+++ DI+GAE
Sbjct: 307 GRNR-IPYHYLLSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDIEGAE 366

Query: 339 KILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENGKEPQASTWDRLATGY 398
           K+ +EW    + +D +IPN+++N+Y K   ++ AE     ++E G +P +STW+ LA G+
Sbjct: 367 KVYEEWLPVKSSYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEILAVGH 426

Query: 399 HANGQTMKAVETMKKAISA-SQPVWKPNYYTLAACLEYLKTNGNVEVAEEIIALLQKHNN 458
                  +A+  ++ A SA     W+P    L+   +  +   +V   E ++ LL++  +
Sbjct: 427 TRKRCISEALTCLRNAFSAEGSSNWRPKVLMLSGFFKLCEEESDVTSKEAVLELLRQSGD 486

Query: 459 VSKNICNRLVDYIHSENETSNALDQLGLDGQIERL 491
           +       L+D   +    ++ +D    D  + +L
Sbjct: 487 LEDKSYLALIDVDENRTVNNSEIDAHETDALLTQL 520

BLAST of Lag0015081 vs. ExPASy Swiss-Prot
Match: Q9SY07 (Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g02820 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 9.7e-68
Identity = 149/428 (34.81%), Postives = 247/428 (57.71%), Query Frame = 0

Query: 39  DSLYRRVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWIS 98
           D+L  R+      + S V  + +W EEG  V++ +L +++++LRK +R+ HAL++CEW+ 
Sbjct: 61  DTLGGRLLSLVYTKRSAVVTIRKWKEEGHSVRKYELNRIVRELRKIKRYKHALEICEWMV 120

Query: 99  NERNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEK 158
            + +     GD A+ L LISK+ GL  AEK+F  + +  R +    +LL+ YV++K  +K
Sbjct: 121 VQEDIKLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTSLLHSYVQNKLSDK 180

Query: 159 AEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMN 218
           AEAL EKM E GF+K+ LPYN ML++Y   G+ EK+  L++E+ ++  + D  TYN+ + 
Sbjct: 181 AEALFEKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKEL-KIRTSPDIVTYNLWLT 240

Query: 219 AYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGD 278
           A+A+ +++   EK+ LK + + L   DW  Y V+ N Y K    EK+ + LK  E+L+  
Sbjct: 241 AFASGNDVEGAEKVYLKAKEEKL-NPDWVTYSVLTNLYAKTDNVEKARLALKEMEKLVSK 300

Query: 279 KQKWFAYECLITLYAAIGNKDEVYRVW-NLYTNLKRRFNTGYLCIISSLMKLDDIDGAEK 338
           K +  AY  LI+L+A +G+KD V   W  + ++ K+  +  YL +IS+++KL + + A+ 
Sbjct: 301 KNR-VAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMISAVVKLGEFEQAKG 360

Query: 339 ILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENGKEPQASTWDRLATGYH 398
           +  EWES     D +IPN+++  Y  +  V   E++  R++E G  P  STW+ L   Y 
Sbjct: 361 LYDEWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGINPSYSTWEILTWAYL 420

Query: 399 ANGQTMKAVETMKKAISASQPVWKPNYYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVS 458
                 K ++   KAI  S   W  N   +    + L+  GNV+ AE+++ LLQK   V+
Sbjct: 421 KRKDMEKVLDCFGKAID-SVKKWTVNVRLVKGACKELEEQGNVKGAEKLMTLLQKAGYVN 480

Query: 459 KNICNRLV 466
             + N L+
Sbjct: 481 TQLYNSLL 484

BLAST of Lag0015081 vs. ExPASy Swiss-Prot
Match: Q9C7F1 (Putative pentatricopeptide repeat-containing protein At1g28020 OS=Arabidopsis thaliana OX=3702 GN=At1g28020 PE=3 SV=2)

HSP 1 Score: 256.1 bits (653), Expect = 8.2e-67
Identity = 160/468 (34.19%), Postives = 255/468 (54.49%), Query Frame = 0

Query: 44  RVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNH 103
           R++ A      I+ VL+QW ++G +V  S ++ +IK+LR   +   ALQ+ EW+S E+  
Sbjct: 41  RITDALHRNAQIIPVLEQWRQQGNQVNPSHVRVIIKKLRDSDQSLQALQVSEWMSKEKIC 100

Query: 104 DPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKVYGALLNCYV-EDKNLEKAEAL 163
           +  P D A +LHLI  V GLE+AEK+F SI +++R   VY +LLN Y   DK L KAEA 
Sbjct: 101 NLIPEDFAARLHLIENVVGLEEAEKFFESIPKNARGDSVYTSLLNSYARSDKTLCKAEAT 160

Query: 164 MEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAA 223
            +KMR++G +  P+PYN M++LY+ L   EK++EL+ EM++  +  D  T N  +  Y+A
Sbjct: 161 FQKMRDLGLLLRPVPYNAMMSLYSALKNREKVEELLLEMKDNDVEADNVTVNNVLKLYSA 220

Query: 224 TSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKW 283
             ++T MEK L K E    I ++WH    +A  Y +   S K++ ML+ +EQL+  K   
Sbjct: 221 VCDVTEMEKFLNKWEGIHGIKLEWHTTLDMAKAYLRARSSGKAMKMLRLTEQLVDQKSLK 280

Query: 284 FAYECLITLYAAIGNKDEVYRVWNLY-TNLKRRFNTGYLCIISSLMKLDDIDGAEKILKE 343
            AY+ L+ LY   GN++EV RVW LY + +  R N GY  +I SL+K+DDI GAE+I K 
Sbjct: 281 SAYDHLMKLYGEAGNREEVLRVWKLYKSKIGERDNNGYRTVIRSLLKVDDIVGAEEIYKV 340

Query: 344 WESGDTCFDFKIPNMMINSYCKKGLVDKAEEYIS------RLMENGKEPQASTW-DRL-A 403
           WES    FD +IP M+ + Y  +G+ +KAE+ ++      R M     P    W D++  
Sbjct: 341 WESLPLEFDHRIPTMLASGYRDRGMTEKAEKLMNSKTIKDRRMNKPVTPLLEQWGDQMKP 400

Query: 404 TGYHANGQTMKAVETMKKAISASQPVWKPNYYTLAACLEYLKTNGNVEVAEEIIALLQKH 463
           +      + ++  +   KA+  S+ + +        C  YL+         E +  L++ 
Sbjct: 401 SDLKCLIKNLRDSKQFSKALQVSEWMGEKQ-----VCNLYLEDYAARLYLTENVLGLEEA 460

Query: 464 NNVSKNICNRLVDY-IHSENETSNALDQLGLDGQIERLDHPSDQNKLD 501
               +NI   + DY ++    +S A     L   ++ +    ++N +D
Sbjct: 461 EKYFENIPENMKDYSVYVALLSSYAKSDKNLGNMVDEILREMEENNVD 503

BLAST of Lag0015081 vs. ExPASy TrEMBL
Match: A0A6J1D3H6 (pentatricopeptide repeat-containing protein At2g20710, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016662 PE=4 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 5.2e-258
Identity = 444/509 (87.23%), Postives = 476/509 (93.52%), Query Frame = 0

Query: 4   LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
           +KLHCSQPWR CC SK F+AL YSTK   SS SPEDSLYRRVSQAGDPRISI RVLDQWV
Sbjct: 2   MKLHCSQPWRGCCTSKAFRALFYSTKALTSSPSPEDSLYRRVSQAGDPRISIRRVLDQWV 61

Query: 64  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
           EEGR VK SDLQKLIKQLRKFRRFNHALQLCEWISNE NHDPSPGDIAI+LHLISKV+GL
Sbjct: 62  EEGRLVKISDLQKLIKQLRKFRRFNHALQLCEWISNEMNHDPSPGDIAIRLHLISKVYGL 121

Query: 124 EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
           EQAEKYFSSI ESSRDY+VYGALLNCYVED++LEKAE +M+KMRE+GFMKTPL +NVML+
Sbjct: 122 EQAEKYFSSINESSRDYRVYGALLNCYVEDRDLEKAEEIMQKMRELGFMKTPLSFNVMLS 181

Query: 184 LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
           LYAHLGKHEKLD LMEEMEEMGIAHDRFTYNIRMNA+AATSNITNMEKLLLKMEAD LIT
Sbjct: 182 LYAHLGKHEKLDALMEEMEEMGIAHDRFTYNIRMNAHAATSNITNMEKLLLKMEADRLIT 241

Query: 244 MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
           MDWHAY+VVANGYFK GLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNK EVYR
Sbjct: 242 MDWHAYYVVANGYFKAGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKAEVYR 301

Query: 304 VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
           VWNLYTNLKRR+NT YLCIISSLMKLDDI+GAEKILKEWESGDTCFDFKIPNMMIN YC+
Sbjct: 302 VWNLYTNLKRRYNTAYLCIISSLMKLDDIEGAEKILKEWESGDTCFDFKIPNMMINIYCR 361

Query: 364 KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
           KGLVDKAE YISRLME+GKEPQA+TWDRLATGYHANGQTMKAVET+KKAISASQP WKPN
Sbjct: 362 KGLVDKAEAYISRLMESGKEPQANTWDRLATGYHANGQTMKAVETIKKAISASQPGWKPN 421

Query: 424 YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETSNALDQLGL 483
            +TLAACLE+LKTN NVEVAEEII LL+KH+ VS  IC+ LVDY+HSE +TS+ALDQLGL
Sbjct: 422 DHTLAACLEFLKTNENVEVAEEIIRLLRKHDIVSIRICDGLVDYVHSEIQTSSALDQLGL 481

Query: 484 DGQIERLDHPSDQNKLDLAELKYEETSDS 513
           DGQIER +H SD+NKLD+AE+KYEETSDS
Sbjct: 482 DGQIERHNHASDRNKLDIAEVKYEETSDS 510

BLAST of Lag0015081 vs. ExPASy TrEMBL
Match: A0A6J1JMF8 (pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111485992 PE=4 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.4e-255
Identity = 436/508 (85.83%), Postives = 467/508 (91.93%), Query Frame = 0

Query: 4   LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
           +KLH  QPWRC CNS++ + L YST G  S RSPEDSLYRRVSQAGDPRISIV VLDQWV
Sbjct: 2   MKLHSLQPWRCYCNSRILRPLFYSTLGSTSFRSPEDSLYRRVSQAGDPRISIVSVLDQWV 61

Query: 64  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
           EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERN +PSPGDIAIQLHLISKVHGL
Sbjct: 62  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNQEPSPGDIAIQLHLISKVHGL 121

Query: 124 EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
           EQAEKYF+SI ESSRDYKVYGALLNCYVEDK LEKAEALMEKMRE+GF+KTPLPYN ML 
Sbjct: 122 EQAEKYFNSIRESSRDYKVYGALLNCYVEDKKLEKAEALMEKMRELGFVKTPLPYNTMLG 181

Query: 184 LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
           LYAHLGKHEKLDEL+EEMEEMGIA DRFTYNIRMNAYAA S++TNMEKLLLKMEADPLI+
Sbjct: 182 LYAHLGKHEKLDELIEEMEEMGIARDRFTYNIRMNAYAANSDVTNMEKLLLKMEADPLIS 241

Query: 244 MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
           MDWHAYFVVANGYFK GLS+KS++MLKRSEQLIGDKQ+WFAYECLITLYAAIGNKD+VYR
Sbjct: 242 MDWHAYFVVANGYFKAGLSDKSLLMLKRSEQLIGDKQRWFAYECLITLYAAIGNKDQVYR 301

Query: 304 VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
           VWNLY NLKRR+NTGYLCIISSLMKLDDI+GAE+ILKEWESGDTCFDFKIPNMM+NSYCK
Sbjct: 302 VWNLYANLKRRYNTGYLCIISSLMKLDDIEGAEEILKEWESGDTCFDFKIPNMMVNSYCK 361

Query: 364 KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
           KGLVDKAE YISRLMENGKEPQA+TWDRLATGYHAN QTMKAVE MKKAISAS P WKPN
Sbjct: 362 KGLVDKAEAYISRLMENGKEPQANTWDRLATGYHANDQTMKAVEAMKKAISASPPGWKPN 421

Query: 424 YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETSNALDQLGL 483
           YYTLAACLEYLKTNGNVE  EEI+ LL KHN +S +I +R VDYIHS+N+TS+ALDQ GL
Sbjct: 422 YYTLAACLEYLKTNGNVEAVEEIVRLLGKHNIISSHINDRFVDYIHSDNQTSSALDQFGL 481

Query: 484 DGQIERLDHPSDQNKLDLAE-LKYEETS 511
           D  IER+DH SD NKL+ AE LK EETS
Sbjct: 482 DDHIERIDHASDLNKLNFAEVLKNEETS 509

BLAST of Lag0015081 vs. ExPASy TrEMBL
Match: A0A6J1EXP1 (pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111437133 PE=4 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.5e-254
Identity = 435/511 (85.13%), Postives = 468/511 (91.59%), Query Frame = 0

Query: 4   LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
           +KLH  QPWRC CNS++ + L YST G  S RSPEDSLY RVSQAGDPRISIV VLDQWV
Sbjct: 2   MKLHSLQPWRCYCNSRILRPLFYSTLGSTSFRSPEDSLYLRVSQAGDPRISIVSVLDQWV 61

Query: 64  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
           EEGREVKQSDLQKLIKQLR+FRRFNHALQLCEWISNERN +PSPGDIAIQLHLISKVHGL
Sbjct: 62  EEGREVKQSDLQKLIKQLRRFRRFNHALQLCEWISNERNQEPSPGDIAIQLHLISKVHGL 121

Query: 124 EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
           EQAEKYF+SI ESSRDYKVYGALLNCYVEDK LEK+EALMEKMRE+GF+KTPLPYN ML 
Sbjct: 122 EQAEKYFNSIRESSRDYKVYGALLNCYVEDKKLEKSEALMEKMRELGFLKTPLPYNTMLG 181

Query: 184 LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
           LYAHLGKHEKLDEL+EEMEEMGIA DRFTYNIRMNAYAA S++TNMEKLLLKMEADPLI+
Sbjct: 182 LYAHLGKHEKLDELIEEMEEMGIARDRFTYNIRMNAYAANSDVTNMEKLLLKMEADPLIS 241

Query: 244 MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
           MDWHAYFVVANGYFK GLS+KS++MLKRSEQLIGDKQ+WFAYECLITLYAAIGNKD+VYR
Sbjct: 242 MDWHAYFVVANGYFKAGLSDKSLLMLKRSEQLIGDKQRWFAYECLITLYAAIGNKDQVYR 301

Query: 304 VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
           VWNLY NLKRR+NTGYLCIISSLMKLDDI+GAE+ILKEWESGDT FDFKIPNMM+NSYCK
Sbjct: 302 VWNLYANLKRRYNTGYLCIISSLMKLDDIEGAEEILKEWESGDTRFDFKIPNMMVNSYCK 361

Query: 364 KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
           KGLVDKAE YI RLME+GKEPQA+TWDRLATGYHAN QTMKAVE MKKAISAS P WKPN
Sbjct: 362 KGLVDKAEAYIGRLMEDGKEPQANTWDRLATGYHANDQTMKAVEAMKKAISASPPGWKPN 421

Query: 424 YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETSNALDQLGL 483
           YYTLAACLEYLKTNGNVEVAEEI+ LL KHN +S +I  RLVDY HS+N+TS+ALDQ GL
Sbjct: 422 YYTLAACLEYLKTNGNVEVAEEIVRLLGKHNIISSHINERLVDYTHSDNQTSSALDQFGL 481

Query: 484 DGQIERLDHPSDQNKLDLAE-LKYEETSDSK 514
           DG IER+DH SD NKL+ AE LK EETSD K
Sbjct: 482 DGHIERIDHASDLNKLNFAEVLKNEETSDMK 512

BLAST of Lag0015081 vs. ExPASy TrEMBL
Match: A0A1S4E0B6 (pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103495690 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.3e-221
Identity = 382/493 (77.48%), Postives = 422/493 (85.60%), Query Frame = 0

Query: 4   LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
           +KLHCSQ W    N K+ QAL YSTK  PSSRS ED+L+RRV +AGDPRISIVRVLDQW+
Sbjct: 41  MKLHCSQSWLFSSNFKVLQALFYSTKSLPSSRSTEDTLFRRVFRAGDPRISIVRVLDQWI 100

Query: 64  EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
           EEGR+V QSD+Q LIKQLRKF RFNHALQLCEWI NERN +PSPGDIA+QLHLISK  GL
Sbjct: 101 EEGRKVNQSDIQALIKQLRKFGRFNHALQLCEWIHNERNKNPSPGDIAVQLHLISKARGL 160

Query: 124 EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
           EQAEKYFSSI ESSRD+KVYGALLNCYVE+KNLEKAEA+M+KMREVGFMKTPL YNVML+
Sbjct: 161 EQAEKYFSSIRESSRDHKVYGALLNCYVENKNLEKAEAIMQKMREVGFMKTPLSYNVMLS 220

Query: 184 LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
           LYA LGK EK DEL++EMEEMGI HDRFTYNIRMNAYAATS+I NMEKLL KMEAD L+ 
Sbjct: 221 LYADLGKQEKFDELIKEMEEMGIGHDRFTYNIRMNAYAATSDIANMEKLLSKMEADSLVA 280

Query: 244 MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
           MDWHAYF V NGY K G SE  I+MLK++EQLIGDKQKW AYE LITLY AIGNKDEVYR
Sbjct: 281 MDWHAYFTVGNGYLKAGFSENGILMLKKAEQLIGDKQKWSAYEYLITLYGAIGNKDEVYR 340

Query: 304 VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
           VWNLY+NL++RFN+GYLC+I+SLMKLDDIDGAE+ILKEWESGDTCFDF+IPNMMINSYC 
Sbjct: 341 VWNLYSNLEKRFNSGYLCMINSLMKLDDIDGAERILKEWESGDTCFDFRIPNMMINSYCT 400

Query: 364 KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
           KG +DKAE YISRL+ENGKEP+A  WDRL +GYH+NG T KA ETMKKAIS S P WKPN
Sbjct: 401 KGFMDKAEAYISRLIENGKEPRAFAWDRLVSGYHSNGLTNKAAETMKKAISVSPPRWKPN 460

Query: 424 YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETS-NALDQLG 483
            + +AACLEYLKTNGNVE+AEEII LL K +    NICNRL DYIHSEN+TS   LD L 
Sbjct: 461 NHIVAACLEYLKTNGNVELAEEIIGLLCKGDIFPSNICNRLEDYIHSENQTSIKCLDLLD 520

Query: 484 LDGQIERLDHPSD 496
           L GQ E LDH  D
Sbjct: 521 LKGQSEGLDHELD 533

BLAST of Lag0015081 vs. ExPASy TrEMBL
Match: A0A5A7SQP0 (Putative pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003480 PE=4 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.1e-220
Identity = 380/493 (77.08%), Postives = 422/493 (85.60%), Query Frame = 0

Query: 4    LKLHCSQPWRCCCNSKLFQALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWV 63
            +KLHCSQ W    N K+ QAL YSTK  PSSRS ED+L+RRV +AGDPRISIVRVLDQW+
Sbjct: 531  MKLHCSQSWLFSSNFKVLQALFYSTKSLPSSRSTEDTLFRRVFRAGDPRISIVRVLDQWI 590

Query: 64   EEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGL 123
            EEGR+V QSD+Q LIKQLRKF RFNHALQLCEWI NERN +PSPGDIA+QLHLISK  GL
Sbjct: 591  EEGRKVNQSDIQALIKQLRKFGRFNHALQLCEWIHNERNKNPSPGDIAVQLHLISKARGL 650

Query: 124  EQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLN 183
            EQAEKYFSSI ESSRD+KVYGALLNCYVE+KNLEKAEA+M+KMREVGFMKTPL YNVML+
Sbjct: 651  EQAEKYFSSIRESSRDHKVYGALLNCYVENKNLEKAEAIMQKMREVGFMKTPLSYNVMLS 710

Query: 184  LYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLIT 243
            LYA LGK EK DEL++EMEEMGI HDRFTYNIRMNAYAATS+I NMEKLL KMEAD L+ 
Sbjct: 711  LYADLGKQEKFDELIKEMEEMGIGHDRFTYNIRMNAYAATSDIANMEKLLSKMEADSLVA 770

Query: 244  MDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYR 303
            MDWH+YF V NGY K G SE  I+MLK++EQLIGDKQKW AYE LITLY AIGNKDEVYR
Sbjct: 771  MDWHSYFTVGNGYLKAGFSENGILMLKKAEQLIGDKQKWSAYEYLITLYGAIGNKDEVYR 830

Query: 304  VWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCK 363
            VWNLY+NL++RFN+GYLC+I+SLMKLDDIDGAE+ILKEWESGDTCFDF+IPNMMINSYC 
Sbjct: 831  VWNLYSNLEKRFNSGYLCMINSLMKLDDIDGAERILKEWESGDTCFDFRIPNMMINSYCT 890

Query: 364  KGLVDKAEEYISRLMENGKEPQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPN 423
            KG +DKAE YISRL+ENGKEP+A  WDRL +GYH+NG T KA ET+KKAIS S P WKPN
Sbjct: 891  KGFMDKAEAYISRLIENGKEPRAFAWDRLVSGYHSNGLTNKAAETLKKAISVSPPRWKPN 950

Query: 424  YYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVSKNICNRLVDYIHSENETS-NALDQLG 483
             + +AACLEYLKTNGNVE+AEEII LL K +    NICNRL DYIHSEN+TS   LD L 
Sbjct: 951  NHIVAACLEYLKTNGNVELAEEIIGLLCKGDIFPSNICNRLEDYIHSENQTSIKCLDLLD 1010

Query: 484  LDGQIERLDHPSD 496
            L GQ E LDH  D
Sbjct: 1011 LKGQSEGLDHELD 1023

BLAST of Lag0015081 vs. TAIR 10
Match: AT2G20710.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 437.2 bits (1123), Expect = 1.8e-122
Identity = 204/434 (47.00%), Postives = 306/434 (70.51%), Query Frame = 0

Query: 24  LLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLRK 83
           L +S K  PS   P D+L RRV+++GDP  SI++VLD W+++G  VK S+L  +IK LRK
Sbjct: 23  LFHSGKTTPSPLDPYDTLQRRVARSGDPSASIIKVLDGWLDQGNLVKTSELHSIIKMLRK 82

Query: 84  FRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKVY 143
           F RF+HALQ+ +W+S  R H+ S GD+AI+L LI+KV GL +AEK+F +I    R+Y +Y
Sbjct: 83  FSRFSHALQISDWMSEHRVHEISEGDVAIRLDLIAKVGGLGEAEKFFETIPMERRNYHLY 142

Query: 144 GALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEMEE 203
           GALLNCY   K L KAE + ++M+E+GF+K  LPYNVMLNLY   GK+  +++L+ EME+
Sbjct: 143 GALLNCYASKKVLHKAEQVFQEMKELGFLKGCLPYNVMLNLYVRTGKYTMVEKLLREMED 202

Query: 204 MGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLSE 263
             +  D FT N R++AY+  S++  MEK L++ EAD  + +DW  Y   ANGY K GL+E
Sbjct: 203 ETVKPDIFTVNTRLHAYSVVSDVEGMEKFLMRCEADQGLHLDWRTYADTANGYIKAGLTE 262

Query: 264 KSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYRVWNLYTNLKRRFNTGYLCII 323
           K++ ML++SEQ++  +++  AYE L++ Y A G K+EVYR+W+LY  L   +NTGY+ +I
Sbjct: 263 KALEMLRKSEQMVNAQKRKHAYEVLMSFYGAAGKKEEVYRLWSLYKELDGFYNTGYISVI 322

Query: 324 SSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENGKE 383
           S+L+K+DDI+  EKI++EWE+G + FD +IP+++I  YCKKG+++KAEE ++ L++  + 
Sbjct: 323 SALLKMDDIEEVEKIMEEWEAGHSLFDIRIPHLLITGYCKKGMMEKAEEVVNILVQKWRV 382

Query: 384 PQASTWDRLATGYHANGQTMKAVETMKKAISASQPVWKPNYYTLAACLEYLKTNGNVEVA 443
              STW+RLA GY   G+  KAVE  K+AI  S+P W+P+   L +C++YL+   ++E  
Sbjct: 383 EDTSTWERLALGYKMAGKMEKAVEKWKRAIEVSKPGWRPHQVVLMSCVDYLEGQRDMEGL 442

Query: 444 EEIIALLQKHNNVS 458
            +I+ LL +  ++S
Sbjct: 443 RKILRLLSERGHIS 456

BLAST of Lag0015081 vs. TAIR 10
Match: AT2G20710.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 359.0 bits (920), Expect = 6.4e-99
Identity = 166/361 (45.98%), Postives = 253/361 (70.08%), Query Frame = 0

Query: 97  ISNERNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKVYGALLNCYVEDKNL 156
           +S  R H+ S GD+AI+L LI+KV GL +AEK+F +I    R+Y +YGALLNCY   K L
Sbjct: 1   MSEHRVHEISEGDVAIRLDLIAKVGGLGEAEKFFETIPMERRNYHLYGALLNCYASKKVL 60

Query: 157 EKAEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIR 216
            KAE + ++M+E+GF+K  LPYNVMLNLY   GK+  +++L+ EME+  +  D FT N R
Sbjct: 61  HKAEQVFQEMKELGFLKGCLPYNVMLNLYVRTGKYTMVEKLLREMEDETVKPDIFTVNTR 120

Query: 217 MNAYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLI 276
           ++AY+  S++  MEK L++ EAD  + +DW  Y   ANGY K GL+EK++ ML++SEQ++
Sbjct: 121 LHAYSVVSDVEGMEKFLMRCEADQGLHLDWRTYADTANGYIKAGLTEKALEMLRKSEQMV 180

Query: 277 GDKQKWFAYECLITLYAAIGNKDEVYRVWNLYTNLKRRFNTGYLCIISSLMKLDDIDGAE 336
             +++  AYE L++ Y A G K+EVYR+W+LY  L   +NTGY+ +IS+L+K+DDI+  E
Sbjct: 181 NAQKRKHAYEVLMSFYGAAGKKEEVYRLWSLYKELDGFYNTGYISVISALLKMDDIEEVE 240

Query: 337 KILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENGKEPQASTWDRLATGY 396
           KI++EWE+G + FD +IP+++I  YCKKG+++KAEE ++ L++  +    STW+RLA GY
Sbjct: 241 KIMEEWEAGHSLFDIRIPHLLITGYCKKGMMEKAEEVVNILVQKWRVEDTSTWERLALGY 300

Query: 397 HANGQTMKAVETMKKAISASQPVWKPNYYTLAACLEYLKTNGNVEVAEEIIALLQKHNNV 456
              G+  KAVE  K+AI  S+P W+P+   L +C++YL+   ++E   +I+ LL +  ++
Sbjct: 301 KMAGKMEKAVEKWKRAIEVSKPGWRPHQVVLMSCVDYLEGQRDMEGLRKILRLLSERGHI 360

Query: 457 S 458
           S
Sbjct: 361 S 361

BLAST of Lag0015081 vs. TAIR 10
Match: AT4G21705.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 9.2e-82
Identity = 158/446 (35.43%), Postives = 266/446 (59.64%), Query Frame = 0

Query: 23  ALLYSTKGFPSSRSPEDSLYRRVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLR 82
           A L +++ + ++R  + +LY ++S  GDP+ S+   L  WV+ G++V  ++L +++  LR
Sbjct: 9   ANLIASRYYYTNRVKKTTLYSKISPLGDPKSSVYPELQNWVQCGKKVSVAELIRIVHDLR 68

Query: 83  KFRRFNHALQLCEWISNERNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKV 142
           + +RF HAL++ +W++       SP + A+ L LI +V+G   AE+YF ++ E  ++ K 
Sbjct: 69  RRKRFLHALEVSKWMNETGVCVFSPTEHAVHLDLIGRVYGFVTAEEYFENLKEQYKNDKT 128

Query: 143 YGALLNCYVEDKNLEKAEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEME 202
           YGALLNCYV  +N+EK+    EKM+E+GF+ + L YN ++ LY ++G+HEK+ +++EEM+
Sbjct: 129 YGALLNCYVRQQNVEKSLLHFEKMKEMGFVTSSLTYNNIMCLYTNIGQHEKVPKVLEEMK 188

Query: 203 EMGIAHDRFTYNIRMNAYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLS 262
           E  +A D ++Y I +NA+ A  ++  +   L  ME    ITMDW+ Y V A  Y   G  
Sbjct: 189 EENVAPDNYSYRICINAFGAMYDLERIGGTLRDMERRQDITMDWNTYAVAAKFYIDGGDC 248

Query: 263 EKSIMMLKRSEQLIGDKQKWFAYECLITLYAAIGNKDEVYRVWNLYTNL-KRRFNTGYLC 322
           ++++ +LK SE  + +K+    Y  LITLYA +G K EV R+W+L  ++ KRR N  YL 
Sbjct: 249 DRAVELLKMSENRL-EKKDGEGYNHLITLYARLGKKIEVLRLWDLEKDVCKRRINQDYLT 308

Query: 323 IISSLMKLDDIDGAEKILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENG 382
           ++ SL+K+D +  AE++L EW+S   C+DF++PN +I  Y  K + +KAE  +  L   G
Sbjct: 309 VLQSLVKIDALVEAEEVLTEWKSSGNCYDFRVPNTVIRGYIGKSMEEKAEAMLEDLARRG 368

Query: 383 KEPQASTWDRLATGYHANGQTMKAVETMKKA--ISASQPVWKPNYYTLAACLEYLKTNGN 442
           K     +W+ +AT Y   G    A + MK A  +      W+P    + + L ++   G+
Sbjct: 369 KATTPESWELVATAYAEKGTLENAFKCMKTALGVEVGSRKWRPGLTLVTSVLSWVGDEGS 428

Query: 443 VEVAEEIIALLQKHNNVSKNICNRLV 466
           ++  E  +A L+    V+K + + LV
Sbjct: 429 LKEVESFVASLRNCIGVNKQMYHALV 453

BLAST of Lag0015081 vs. TAIR 10
Match: AT1G02150.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 284.6 bits (727), Expect = 1.5e-76
Identity = 148/455 (32.53%), Postives = 271/455 (59.56%), Query Frame = 0

Query: 39  DSLYRRVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWIS 98
           +++Y+++S    P +    VL+QW + GR++ + +L +++K+LRK++R N AL++ +W++
Sbjct: 67  NAIYKKISLMEKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKRANQALEVYDWMN 126

Query: 99  NE-RNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLE 158
           N       S  D AIQL LI KV G+  AE++F  + E+ +D +VYG+LLN YV  K+ E
Sbjct: 127 NRGERFRLSASDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKSRE 186

Query: 159 KAEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRM 218
           KAEAL+  MR+ G+   PLP+NVM+ LY +L +++K+D ++ EM++  I  D ++YNI +
Sbjct: 187 KAEALLNTMRDKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNIWL 246

Query: 219 NAYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIG 278
           ++  +  ++  ME +  +M++D  I  +W  +  +A  Y K+G +EK+   L++ E  I 
Sbjct: 247 SSCGSLGSVEKMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEARIT 306

Query: 279 DKQKWFAYECLITLYAAIGNKDEVYRVWNLYTNLKRRF-NTGYLCIISSLMKLDDIDGAE 338
            + +   Y  L++LY ++GNK E+YRVW++Y ++     N GY  ++SSL+++ DI+GAE
Sbjct: 307 GRNR-IPYHYLLSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDIEGAE 366

Query: 339 KILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENGKEPQASTWDRLATGY 398
           K+ +EW    + +D +IPN+++N+Y K   ++ AE     ++E G +P +STW+ LA G+
Sbjct: 367 KVYEEWLPVKSSYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEILAVGH 426

Query: 399 HANGQTMKAVETMKKAISA-SQPVWKPNYYTLAACLEYLKTNGNVEVAEEIIALLQKHNN 458
                  +A+  ++ A SA     W+P    L+   +  +   +V   E ++ LL++  +
Sbjct: 427 TRKRCISEALTCLRNAFSAEGSSNWRPKVLMLSGFFKLCEEESDVTSKEAVLELLRQSGD 486

Query: 459 VSKNICNRLVDYIHSENETSNALDQLGLDGQIERL 491
           +       L+D   +    ++ +D    D  + +L
Sbjct: 487 LEDKSYLALIDVDENRTVNNSEIDAHETDALLTQL 520

BLAST of Lag0015081 vs. TAIR 10
Match: AT4G02820.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 6.9e-69
Identity = 149/428 (34.81%), Postives = 247/428 (57.71%), Query Frame = 0

Query: 39  DSLYRRVSQAGDPRISIVRVLDQWVEEGREVKQSDLQKLIKQLRKFRRFNHALQLCEWIS 98
           D+L  R+      + S V  + +W EEG  V++ +L +++++LRK +R+ HAL++CEW+ 
Sbjct: 61  DTLGGRLLSLVYTKRSAVVTIRKWKEEGHSVRKYELNRIVRELRKIKRYKHALEICEWMV 120

Query: 99  NERNHDPSPGDIAIQLHLISKVHGLEQAEKYFSSIMESSRDYKVYGALLNCYVEDKNLEK 158
            + +     GD A+ L LISK+ GL  AEK+F  + +  R +    +LL+ YV++K  +K
Sbjct: 121 VQEDIKLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTSLLHSYVQNKLSDK 180

Query: 159 AEALMEKMREVGFMKTPLPYNVMLNLYAHLGKHEKLDELMEEMEEMGIAHDRFTYNIRMN 218
           AEAL EKM E GF+K+ LPYN ML++Y   G+ EK+  L++E+ ++  + D  TYN+ + 
Sbjct: 181 AEALFEKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKEL-KIRTSPDIVTYNLWLT 240

Query: 219 AYAATSNITNMEKLLLKMEADPLITMDWHAYFVVANGYFKVGLSEKSIMMLKRSEQLIGD 278
           A+A+ +++   EK+ LK + + L   DW  Y V+ N Y K    EK+ + LK  E+L+  
Sbjct: 241 AFASGNDVEGAEKVYLKAKEEKL-NPDWVTYSVLTNLYAKTDNVEKARLALKEMEKLVSK 300

Query: 279 KQKWFAYECLITLYAAIGNKDEVYRVW-NLYTNLKRRFNTGYLCIISSLMKLDDIDGAEK 338
           K +  AY  LI+L+A +G+KD V   W  + ++ K+  +  YL +IS+++KL + + A+ 
Sbjct: 301 KNR-VAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMISAVVKLGEFEQAKG 360

Query: 339 ILKEWESGDTCFDFKIPNMMINSYCKKGLVDKAEEYISRLMENGKEPQASTWDRLATGYH 398
           +  EWES     D +IPN+++  Y  +  V   E++  R++E G  P  STW+ L   Y 
Sbjct: 361 LYDEWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGINPSYSTWEILTWAYL 420

Query: 399 ANGQTMKAVETMKKAISASQPVWKPNYYTLAACLEYLKTNGNVEVAEEIIALLQKHNNVS 458
                 K ++   KAI  S   W  N   +    + L+  GNV+ AE+++ LLQK   V+
Sbjct: 421 KRKDMEKVLDCFGKAID-SVKKWTVNVRLVKGACKELEEQGNVKGAEKLMTLLQKAGYVN 480

Query: 459 KNICNRLV 466
             + N L+
Sbjct: 481 TQLYNSLL 484

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147816.11.1e-25787.23pentatricopeptide repeat-containing protein At2g20710, mitochondrial [Momordica ... [more]
KAG7022915.11.7e-25585.32Pentatricopeptide repeat-containing protein, mitochondrial [Cucurbita argyrosper... [more]
XP_022988744.12.9e-25585.83pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like [Cucur... [more]
XP_023531849.12.9e-25585.13pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like [Cucur... [more]
KAG6589215.11.5e-25485.13Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
Match NameE-valueIdentityDescription
Q9SKU62.6e-12147.00Pentatricopeptide repeat-containing protein At2g20710, mitochondrial OS=Arabidop... [more]
Q84JR31.3e-8035.43Pentatricopeptide repeat-containing protein At4g21705, mitochondrial OS=Arabidop... [more]
Q8LPS62.1e-7532.53Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX... [more]
Q9SY079.7e-6834.81Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidop... [more]
Q9C7F18.2e-6734.19Putative pentatricopeptide repeat-containing protein At1g28020 OS=Arabidopsis th... [more]
Match NameE-valueIdentityDescription
A0A6J1D3H65.2e-25887.23pentatricopeptide repeat-containing protein At2g20710, mitochondrial OS=Momordic... [more]
A0A6J1JMF81.4e-25585.83pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like OS=Cuc... [more]
A0A6J1EXP13.5e-25485.13pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like OS=Cuc... [more]
A0A1S4E0B62.3e-22177.48pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like OS=Cuc... [more]
A0A5A7SQP01.1e-22077.08Putative pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
AT2G20710.11.8e-12247.00Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G20710.26.4e-9945.98Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G21705.19.2e-8235.43Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G02150.11.5e-7632.53Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G02820.16.9e-6934.81Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 178..221
e-value: 1.2E-7
score: 31.8
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 355..381
e-value: 2.4E-5
score: 24.2
coord: 142..171
e-value: 3.6E-4
score: 20.6
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 355..384
e-value: 2.0E-6
score: 25.6
coord: 142..171
e-value: 2.0E-5
score: 22.4
coord: 178..207
e-value: 3.0E-5
score: 21.9
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 139..173
score: 9.799459
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 350..384
score: 10.490022
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 174..208
score: 9.722731
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 283..489
e-value: 2.4E-17
score: 65.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 43..202
e-value: 6.4E-18
score: 66.9
coord: 203..276
e-value: 3.2E-5
score: 25.5
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 246..415
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 491..513
NoneNo IPR availablePANTHERPTHR45717OS12G0527900 PROTEINcoord: 20..477
NoneNo IPR availablePANTHERPTHR45717:SF10OS10G0501200 PROTEINcoord: 20..477

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0015081.1Lag0015081.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding