Lag0014931 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0014931
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RNase III domain-containing protein
Location	chr12: 5959419 .. 5962560 (+)
RNA-Seq Expression	Lag0014931
Synteny	Lag0014931

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAAGAAGAACACGGAGTAGATTCGTACGGCGGCGCCGGCGAGGTACGGCAGAATCCGGAGGAGATGAGGACCGAGTCGTCGTCGTCGTCGCCGGCGGCGCCGAGTCTGGAGGGAATCGAGGCGATTCTGAATTATGAGTTTCGAGATAAGAGATTGCTGGAAGAGGCTTTCACAGACGCGTCGTATTCACCGGAAAATTGCTCGTCGTACGAGCGATTGGAGTACGTCGGCGATTCGGTGTTGAATTTTCTGATGACGAGAGAGCAATACTTCTCGAATCCGAATCTGTCGCCTGGTTCACTGACTCGGCTCCGAGCTGCAAATGTCGACACCGAAAAGCTTGCTCGTGTTGCGATTACCCATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTCATACAAAAGTAAGTAATTTCAATTTCAATTTCAATTTCAATTTCAATTTCGTTGTTGTGATCGACTCATTAACTTCGATATTAACTCCTCTGTTTTCTCAAAAATAAAAAAAATAAAAAAAATTAAAAACTCTGTTTTCTTCCCAGGTTTTGTTTATAATTAATCTTTGGTTTAATTATATGTTTAGTCCCTCAATTTTAAGAAGTTTCATATAAGGTTCGAACTTCCAATTTTGTTTTCAATAAGTCTCTTAAATTTAAAAGATTTCAAAACTTTTACTTTTTGTGTTCAACAGGTCCTTTACCTTTTAATTATTATTAAGATTGGTTAATTTTTAAACTCTCAAATATATCCAAGATTTATTAGACATTTTTAAAGTTCGACTTTTTTTAAAGTTAGAACGGGATATTACTCTGAAAATTTAGAGACTAGATTGGTAATAAAACCTTAATCTTTAACCAATCGATTGAGCTAGTTATGATAATTCTTTTATTTTATTTTATTTTTTCTAATTTTTAAAAATTAAGCTCAAAGTTAAATTTAAAATTAATCACGTTTTGTTTTTCTTTGGGTAAAAATATATGAGGAACTTTTTCCATCGCCAATTTTTTTTAGTTTCCACTTTTGTCTTTTTTGTTTTCCTTTTTTGGGTAACATATATAAGGAGCTTTTTCGATTACTTTATTTCTACTTTTGGGAATTTTAGAAATAAAATTGGATATCAGTATCTTTTATCTCTTCCCTAAAAATTAAAATTACTTTTGAAATTATTGGTTATTAGAAATAATAAGATACTAAAGGATTGGTATAGTCTATCACTTATTGAATTAAATTAATTAATATTTGTTAATATATTTTATGCTTGGAAGTAAATAAATATTAATTAAGGCATGAAATGTCTTGAAAATTACCTGTAGATTATTTCCATCATCTATTTTATTCTTGTGAAATCTTCAAATCTTTATTTGGTTAAAGATTTAAAAAATAACTTCTAAAACAAAATATTAAAGCTAATCTCTAATTTAAGATATACTAACAGATAAATTTAAGAATTTATGTATAACCCAAATAATTAATATTTAAATGTATAAAATATATTTAAAAATAAGTCTGGAATTTCAATAAGACTCAAATTGTGCTCTTAAGTCTAAAGTCTATTGTTCTTTTTCGTTAGAAAAAAATTTAAATTCTCTTTATATTCTAGCTTTTTTAGAGTCTCTCATAAATGGATAAATTTTGTTTCCTTTATGAGATAAGATTACATTTTAAAATTTAACTTTTTCAATATAATGATAGCTTAACTGTAATTGACATACGTTCAACCTTTGAAGTCAGATATTCGAATTTCACACCTCCAAATGTTATTGAACTAAAAAAAACGTTCTCAATATTATATTATTACTCGACAAAAAAAAAAAAAATCAATTCTAAATGAGAAGATAGGCTAAAAAATAACTATGTAAGTCAACCGTTCTCATTGCGATAAGCATATGAATAAAACTTTTAAAGAAAGAAAATAATGATGATAATAAAATAAAAATAGAAATTAACAAATTCTTAAATAAAAAAATGACAACATCAATGACGTGTGTATAATATATATTTCTCCCACCGACCTTCCGATCTGACACTTGGTCTAGTTCATGAGTCAATATATTTTATATTTCTTTTTTTCTTTTGTTTCTTGAATTAAAATAGGGTTTGAACACATGGAATATAAAATGTTAAGGTTGAATTATTAGTTTAATTGGATAATCATTTGATTTTTTTAAAATTAAGCTTAGTTCCACCTATAATTTCATTTGTTTTGTTATTTACACTCTTTCATATTTTCAAAAACCATATCAAATTTGGAAAATTGAAAAAAGTAGACCCCGTTTGATAACCATTTGGTTTTTGAAAATTAAGTTTATAAACACTACTTCCCTTCACAACTTTCTTTGTTTTGTTATCTACAACCTTTCTATGTGTTTAAAAAACTGAATCAAATTTTGAAAAAAAAAAAATTCAAAAACTTGTTTTTGTTTTTGGAATTTGGCTAAGAATTCAAGTGTCTACTTAACAAAATGAAAAATCATGATAAGCTGGAAATTGAGAGAAAACAAGTATAATTTTCAAAAACCGAATGGTTATCAAACGAGGTCGTAGTTATACACTTTTTAAAGTTAAAAAACCTATATTAAACACAAAAATGAAAGTTCAGGACCAAATAAACATAAACGTGAAAGTTTTACTTACTTAAACTTTGATTATTGATGGAATCTATTTGTGACATAACAGATAGAAGAGTTTGCTCAAAGCATAGAGGCATATCCATTACATTCAAATGGAATGATCAAAGCTCCAAAAATTCTAGCCGACATTGTTGAATCAACCATTGGAGCTGTTTTTGCCGATAGCAACTCTATAGACACTGTTTGGACGGTAATTAAACTATTTGGAAAAAAAAATAAAAATTCCATTCTTAATCATAAATAATCCTCACTTGTATGTATTACATTATTTTGATGAATTAAAGATGATTTATTTATTTTGTAGATATTTAAGGAGCTATTGGAGCCATTGATTACTGTAAAGACCATGAAAAGGCATCCAATGACAGAGTTGACTGAAATTTGCCAAAAAAGAAGTCTCAAGTTGGAATTTGTGGACCTATGGGAAGAAACAAAAGAAATTCAAGTTTTTATAGACAAAAACTTGTGGGAAAAGGAAGCTATTGCAAAAAACTTATAG

mRNA sequence

ATGGAAGAAGAAGAACACGGAGTAGATTCGTACGGCGGCGCCGGCGAGGTACGGCAGAATCCGGAGGAGATGAGGACCGAGTCGTCGTCGTCGTCGCCGGCGGCGCCGAGTCTGGAGGGAATCGAGGCGATTCTGAATTATGAGTTTCGAGATAAGAGATTGCTGGAAGAGGCTTTCACAGACGCGTCGTATTCACCGGAAAATTGCTCGTCGTACGAGCGATTGGAGTACGTCGGCGATTCGGTGTTGAATTTTCTGATGACGAGAGAGCAATACTTCTCGAATCCGAATCTGTCGCCTGGTTCACTGACTCGGCTCCGAGCTGCAAATGTCGACACCGAAAAGCTTGCTCGTGTTGCGATTACCCATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTCATACAAAAATAGAAGAGTTTGCTCAAAGCATAGAGGCATATCCATTACATTCAAATGGAATGATCAAAGCTCCAAAAATTCTAGCCGACATTGTTGAATCAACCATTGGAGCTGTTTTTGCCGATAGCAACTCTATAGACACTGTTTGGACGATATTTAAGGAGCTATTGGAGCCATTGATTACTGTAAAGACCATGAAAAGGCATCCAATGACAGAGTTGACTGAAATTTGCCAAAAAAGAAGTCTCAAGTTGGAATTTGTGGACCTATGGGAAGAAACAAAAGAAATTCAAGTTTTTATAGACAAAAACTTGTGGGAAAAGGAAGCTATTGCAAAAAACTTATAG

Coding sequence (CDS)

ATGGAAGAAGAAGAACACGGAGTAGATTCGTACGGCGGCGCCGGCGAGGTACGGCAGAATCCGGAGGAGATGAGGACCGAGTCGTCGTCGTCGTCGCCGGCGGCGCCGAGTCTGGAGGGAATCGAGGCGATTCTGAATTATGAGTTTCGAGATAAGAGATTGCTGGAAGAGGCTTTCACAGACGCGTCGTATTCACCGGAAAATTGCTCGTCGTACGAGCGATTGGAGTACGTCGGCGATTCGGTGTTGAATTTTCTGATGACGAGAGAGCAATACTTCTCGAATCCGAATCTGTCGCCTGGTTCACTGACTCGGCTCCGAGCTGCAAATGTCGACACCGAAAAGCTTGCTCGTGTTGCGATTACCCATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTCATACAAAAATAGAAGAGTTTGCTCAAAGCATAGAGGCATATCCATTACATTCAAATGGAATGATCAAAGCTCCAAAAATTCTAGCCGACATTGTTGAATCAACCATTGGAGCTGTTTTTGCCGATAGCAACTCTATAGACACTGTTTGGACGATATTTAAGGAGCTATTGGAGCCATTGATTACTGTAAAGACCATGAAAAGGCATCCAATGACAGAGTTGACTGAAATTTGCCAAAAAAGAAGTCTCAAGTTGGAATTTGTGGACCTATGGGAAGAAACAAAAGAAATTCAAGTTTTTATAGACAAAAACTTGTGGGAAAAGGAAGCTATTGCAAAAAACTTATAG

Protein sequence

MEEEEHGVDSYGGAGEVRQNPEEMRTESSSSSPAAPSLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITVKTMKRHPMTELTEICQKRSLKLEFVDLWEETKEIQVFIDKNLWEKEAIAKNL

Homology

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BLAST of Lag0014931 vs. NCBI nr
Match: XP_038888348.1 (ribonuclease 3-like protein 3 [Benincasa hispida])

HSP 1 Score: 377.1 bits (967), Expect = 1.2e-100
Identity = 197/254 (77.56%), Postives = 215/254 (84.65%), Query Frame = 0


Query: 1   MEEEEHGV---DSYGGAGEVRQNPEEMRTESSSSSPAAPSLEGIEAILNYEFRDKRLLEE 60
           MEEEE  V   +  G +G           E+ +   +A SL+GIEAILNYEFRDKRLLEE
Sbjct: 1   MEEEEQRVVFLEEEGSSG---------GGEAVNGGDSAASLDGIEAILNYEFRDKRLLEE 60

Query: 61  AFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLA 120
           AFTDASYSPENCSS+ERLEYVGDSVLNFL+TREQYFSNPNLSPGSLTRLRAANVDTEKLA
Sbjct: 61  AFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLA 120

Query: 121 RVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVESTIGAVFA 180
           RVAITHGFHRYLRHNK DLH KIEEF +SIE YPLHSNGMIKAPK+LADIVESTIGAVFA
Sbjct: 121 RVAITHGFHRYLRHNKPDLHPKIEEFIKSIEDYPLHSNGMIKAPKVLADIVESTIGAVFA 180

Query: 181 DSNSIDTVWTIFKELLEPLITVKTMKRHPMTELTEICQKRSLKLEFVDLWEETKEIQVFI 240
           DSNSI+TVW IFK+L+EPLIT+KTMK+HPMTELTEICQKR+L LEFVD WEE KE+QVFI
Sbjct: 181 DSNSIETVWKIFKDLMEPLITLKTMKKHPMTELTEICQKRNLNLEFVDCWEERKEVQVFI 240

Query: 241 DKNLWEKEAIAKNL 252
           DK L  K +  K L
Sbjct: 241 DKQLVGKGSYRKKL 245

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BLAST of Lag0014931 vs. NCBI nr
Match: XP_004144598.1 (ribonuclease 3-like protein 3 [Cucumis sativus] >KGN43500.1 hypothetical protein Csa_020414 [Cucumis sativus])

HSP 1 Score: 376.3 bits (965), Expect = 2.0e-100
Identity = 201/258 (77.91%), Postives = 216/258 (83.72%), Query Frame = 0


Query: 1   MEEEEHGVD-------SYGGAGEVRQNPEEMRTESSSSSPAAPSLEGIEAILNYEFRDKR 60
           MEE +  VD       S GG GE           + S SPA  SL+GIEAILNYEFRDKR
Sbjct: 1   MEEGKKRVDFLEGKDGSRGGGGE---------AVNGSDSPA--SLDGIEAILNYEFRDKR 60

Query: 61  LLEEAFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAANVDT 120
           LLEEAFTDASYSPENCSS+ERLEYVGDSVLNFL+TREQYFSNPNLSPGSLTRLRAANVDT
Sbjct: 61  LLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDT 120

Query: 121 EKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVESTIG 180
           EKLARVAITHGFHRYLRHNKADLH KIEEF +SIE YPLHSNGMIKAPK+LADIVESTIG
Sbjct: 121 EKLARVAITHGFHRYLRHNKADLHQKIEEFGRSIEEYPLHSNGMIKAPKVLADIVESTIG 180

Query: 181 AVFADSNSIDTVWTIFKELLEPLITVKTMKRHPMTELTEICQKRSLKLEFVDLWEETKEI 240
           AVFADSN IDTVW IFKEL+EPLIT+KTMK+HPMTELTE+CQKRSLKLEF D WEETKEI
Sbjct: 181 AVFADSNFIDTVWKIFKELMEPLITLKTMKKHPMTELTEMCQKRSLKLEFRDRWEETKEI 240

Query: 241 QVFIDKNLWEKEAIAKNL 252
           +V I+K +  K +  K L
Sbjct: 241 EVLIEKQVVGKGSYRKKL 247

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BLAST of Lag0014931 vs. NCBI nr
Match: XP_022969213.1 (ribonuclease 3-like protein 3 [Cucurbita maxima])

HSP 1 Score: 372.9 bits (956), Expect = 2.2e-99
Identity = 188/228 (82.46%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 24  MRTESSSSSPAAPSLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVL 83
           ++ E       A SL+G+EAILNYEFRDKRLLEEAFTDASYSPENCSS+ERLEYVGDSVL
Sbjct: 11  LKQEGRVGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVL 70

Query: 84  NFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEF 143
           NFL+TREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADL  KIEEF
Sbjct: 71  NFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEF 130

Query: 144 AQSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITVKTMK 203
           +++IE YPLHSNGMIKAPKILADIVES IGAVF DSNSIDTVWTIFK L+EPLIT+KTMK
Sbjct: 131 SKNIEEYPLHSNGMIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMK 190

Query: 204 RHPMTELTEICQKRSLKLEFVDLWEETKEIQVFIDKNLWEKEAIAKNL 252
           RHPMTELTE+CQKR+LK EFVDLWEE KEI V IDK L  K +  K L
Sbjct: 191 RHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKL 238

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BLAST of Lag0014931 vs. NCBI nr
Match: XP_023554575.1 (ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 371.7 bits (953), Expect = 5.0e-99
Identity = 187/228 (82.02%), Postives = 201/228 (88.16%), Query Frame = 0


Query: 24  MRTESSSSSPAAPSLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVL 83
           +  E       A SL+G+EAILNYEFRDKRLLEEAFTDASYSPENCSS+ERLEYVGDSVL
Sbjct: 11  LEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVL 70

Query: 84  NFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEF 143
           NFL+TREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADL  KIEEF
Sbjct: 71  NFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEF 130

Query: 144 AQSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITVKTMK 203
           +++IE YPLHSNG+IKAPKILADIVES IGAVF DSNSIDTVWTIFK L+EPLIT+KTMK
Sbjct: 131 SKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMK 190

Query: 204 RHPMTELTEICQKRSLKLEFVDLWEETKEIQVFIDKNLWEKEAIAKNL 252
           RHPMTELTE+CQKR+LK EFVDLWEE KEI V IDK L  K +  K L
Sbjct: 191 RHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKL 238

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BLAST of Lag0014931 vs. NCBI nr
Match: KAG6572281.1 (Endoribonuclease Dicer-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 371.3 bits (952), Expect = 6.5e-99
Identity = 186/228 (81.58%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 24  MRTESSSSSPAAPSLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVL 83
           ++ E       A SL+G+EAILNYEFRDKRLLEEAFTDASYSPENCSS+ERLEYVGDSVL
Sbjct: 11  LKQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVL 70

Query: 84  NFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEF 143
           NFL+TREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNKADL  KIEEF
Sbjct: 71  NFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEF 130

Query: 144 AQSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITVKTMK 203
           +++IE YPLHSNG+IKAPKILADIVES IGAVF DSNSIDTVWTIFK L+EPLIT+KTMK
Sbjct: 131 SKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMK 190

Query: 204 RHPMTELTEICQKRSLKLEFVDLWEETKEIQVFIDKNLWEKEAIAKNL 252
           RHPMTELTE+CQKR+LK EFVDLWEE KEI V IDK L  K +  K L
Sbjct: 191 RHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKL 238

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BLAST of Lag0014931 vs. ExPASy Swiss-Prot
Match: Q69KJ0 (Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0358800 PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.3e-49
Identity = 103/229 (44.98%), Postives = 153/229 (66.81%), Query Frame = 0


Query: 33  PAAP-SLEGIEAILNYEFRDKRLLEEAFTDAS--YSPENCSSYERLEYVGDSVLNFLMTR 92
           P  P  +  +E++L YEFRDK L+EEA T  S  Y      +YERLEY+GD+VL  +++R
Sbjct: 33  PMGPGEVAAVESLLGYEFRDKALVEEALTHGSFYYPYRPGVTYERLEYLGDAVLTCVVSR 92

Query: 93  EQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEA 152
           E + +   L PG LTRLRAANVD EKLARVA+ HG H +LRH   +L  +I +F + +  
Sbjct: 93  EVFLTYGQLQPGPLTRLRAANVDKEKLARVAVVHGLHHFLRHKAPNLDGQITDFIEELSM 152

Query: 153 YPLHSNGMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITVKTMKRHPMT 212
           YP+HSNG++  PK+L D+VES IGA++ DSN + + VW +F++L +PLI+++T+ +HP++
Sbjct: 153 YPIHSNGLLDPPKVLCDVVESLIGAIYCDSNFNQEIVWQVFQKLADPLISLETLGKHPVS 212

Query: 213 ELTEICQKRSLKLEFV-DLWEETKEIQVFIDKNL------WEKEAIAKN 251
           EL E CQK    ++ V D W++   ++V ID  +       +K+ IA+N
Sbjct: 213 ELFEFCQKTRRGVKIVKDEWDKNLTVEVLIDGEMVGRATYAQKKEIAQN 261

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BLAST of Lag0014931 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 163.7 bits (413), Expect = 2.7e-39
Identity = 91/207 (43.96%), Postives = 126/207 (60.87%), Query Frame = 0


Query: 40   GIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLS 99
            G+E  L YEF++K LL EA T AS      S Y+RLE+VGD+VL+ L+TR  +F+  +L 
Sbjct: 1561 GLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLP 1620

Query: 100  PGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIE------AYPLH 159
            PG LT LRAA V+ E  ARVA+ H  H YLRH  + L  +I EF + ++       +   
Sbjct: 1621 PGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSF 1680

Query: 160  SNGMIKAPKILADIVESTIGAVFADSNSIDT-VWTIFKELLEPLITVKTMKRHPMTELTE 219
              G  KAPK+L DIVES  GA+F DS    T  W +F+ LL+P++T +T+  HP+ EL E
Sbjct: 1681 GLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPMHPVRELQE 1740

Query: 220  ICQKRSLKLEF-VDLWEETKEIQVFID 239
             CQ+++  LE+       T  ++VFID
Sbjct: 1741 RCQQQAEGLEYKASRSGNTATVEVFID 1767

[Close]

BLAST of Lag0014931 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 5.6e-37
Identity = 87/210 (41.43%), Postives = 126/210 (60.00%), Query Frame = 0


Query: 37   SLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNP 96
            + + ++ +L  EF++K LL EA T AS      S Y+RLE+VGD+VL+ L+TR  +F+  
Sbjct: 1537 NFDTLKGVLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYT 1596

Query: 97   NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSN- 156
            +L PG LT LRAA V+ E  ARVA+ H  H +LRH  + L T+I EF + ++   L    
Sbjct: 1597 DLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQEELLKPGF 1656

Query: 157  -----GMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITVKTMKRHPMTE 216
                 G  KAPK+L DIVES  GA+F DS      VW +F+ LL P++T +T+  HP+ E
Sbjct: 1657 NSFGLGDCKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPETLPMHPVRE 1716

Query: 217  LTEICQKRSLKLEF-VDLWEETKEIQVFID 239
            L E CQ+++  LE+          ++VF+D
Sbjct: 1717 LQERCQQQAEGLEYKASRAGNIATVEVFVD 1746

[Close]

BLAST of Lag0014931 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.1e-36
Identity = 83/213 (38.97%), Postives = 136/213 (63.85%), Query Frame = 0


Query: 29  SSSSPAAP------SLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSV 88
           S S+P+ P      S+E +E ILNY+F +K LL+EA T  S +  +  SYERLE++GDS 
Sbjct: 45  SPSAPSVPVSSEMESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSA 104

Query: 89  LNFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEE 148
           +   ++   Y + P+L P  L+ LRAANV TEKLARV++ HG + +LR N   L  K++E
Sbjct: 105 IGLAISNYLYLTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKE 164

Query: 149 FAQSI---EAYPLHSNGMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLIT 208
           F++++   +   +   G++KAPK+LAD+ ES  GAV+ D N  +  +W IF+ LLEP++T
Sbjct: 165 FSEAVGKEDDLSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVT 224

Query: 209 VKTMKR--HPMTELTEICQKRSLKLEFVDLWEE 230
           +  +++   P++ L ++C K   +++ +  W++
Sbjct: 225 LDDLQKQPQPVSMLFKLCHKHKKRID-IKNWKD 254

[Close]

BLAST of Lag0014931 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 2.4e-35
Identity = 79/191 (41.36%), Postives = 117/191 (61.26%), Query Frame = 0


Query: 41  IEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLSP 100
           +E +L Y+FRD RLLEEA T  S++ ++  SY+RLE+VGDS L    +   Y +NP L P
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFA-DDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGP 99

Query: 101 GSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYP-------LH 160
           G L+ LRAAN+ TEKLARVA+ H  +  LR N   L   + +F ++++  P        +
Sbjct: 100 GPLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPY 159

Query: 161 SNGMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITVKTMKRHPMTELTE 220
              ++KAPK+LADIVE+   AV+ D    ++ +W + + L EP+IT +T+   P+T L E
Sbjct: 160 GGSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAETIDEQPVTMLHE 219

Query: 221 ICQKRSLKLEF 224
           +CQK     +F
Sbjct: 220 LCQKHGKMAQF 229

[Close]

BLAST of Lag0014931 vs. ExPASy TrEMBL
Match: A0A0A0K1J8 (RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 9.8e-101
Identity = 201/258 (77.91%), Postives = 216/258 (83.72%), Query Frame = 0


Query: 1   MEEEEHGVD-------SYGGAGEVRQNPEEMRTESSSSSPAAPSLEGIEAILNYEFRDKR 60
           MEE +  VD       S GG GE           + S SPA  SL+GIEAILNYEFRDKR
Sbjct: 1   MEEGKKRVDFLEGKDGSRGGGGE---------AVNGSDSPA--SLDGIEAILNYEFRDKR 60

Query: 61  LLEEAFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAANVDT 120
           LLEEAFTDASYSPENCSS+ERLEYVGDSVLNFL+TREQYFSNPNLSPGSLTRLRAANVDT
Sbjct: 61  LLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDT 120

Query: 121 EKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVESTIG 180
           EKLARVAITHGFHRYLRHNKADLH KIEEF +SIE YPLHSNGMIKAPK+LADIVESTIG
Sbjct: 121 EKLARVAITHGFHRYLRHNKADLHQKIEEFGRSIEEYPLHSNGMIKAPKVLADIVESTIG 180

Query: 181 AVFADSNSIDTVWTIFKELLEPLITVKTMKRHPMTELTEICQKRSLKLEFVDLWEETKEI 240
           AVFADSN IDTVW IFKEL+EPLIT+KTMK+HPMTELTE+CQKRSLKLEF D WEETKEI
Sbjct: 181 AVFADSNFIDTVWKIFKELMEPLITLKTMKKHPMTELTEMCQKRSLKLEFRDRWEETKEI 240

Query: 241 QVFIDKNLWEKEAIAKNL 252
           +V I+K +  K +  K L
Sbjct: 241 EVLIEKQVVGKGSYRKKL 247

[Close]

BLAST of Lag0014931 vs. ExPASy TrEMBL
Match: A0A6J1I0C1 (ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 1.1e-99
Identity = 188/228 (82.46%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 24  MRTESSSSSPAAPSLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVL 83
           ++ E       A SL+G+EAILNYEFRDKRLLEEAFTDASYSPENCSS+ERLEYVGDSVL
Sbjct: 11  LKQEGRVGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVL 70

Query: 84  NFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEF 143
           NFL+TREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADL  KIEEF
Sbjct: 71  NFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEF 130

Query: 144 AQSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITVKTMK 203
           +++IE YPLHSNGMIKAPKILADIVES IGAVF DSNSIDTVWTIFK L+EPLIT+KTMK
Sbjct: 131 SKNIEEYPLHSNGMIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMK 190

Query: 204 RHPMTELTEICQKRSLKLEFVDLWEETKEIQVFIDKNLWEKEAIAKNL 252
           RHPMTELTE+CQKR+LK EFVDLWEE KEI V IDK L  K +  K L
Sbjct: 191 RHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKL 238

[Close]

BLAST of Lag0014931 vs. ExPASy TrEMBL
Match: A0A6J1C375 (ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 7.0e-99
Identity = 196/258 (75.97%), Postives = 219/258 (84.88%), Query Frame = 0


Query: 4   EEHGVDSY-------GGA---GEVRQNPEEMRTESSSSSPAAPSLEGIEAILNYEFRDKR 63
           EEHGV+ +        GA    +VR+NP E     SS SP  PSLE +EAIL YEFRDKR
Sbjct: 2   EEHGVELFEQLRLQAQGAVVNDDVRRNPPE-----SSESP--PSLEEVEAILGYEFRDKR 61

Query: 64  LLEEAFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLSPGSLTRLRAANVDT 123
           LLEEAFTD+SYSPENC S+ERLEYVGDSVLNFL+TREQYFSNP+LSPGSLTRLRAANVDT
Sbjct: 62  LLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDT 121

Query: 124 EKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNGMIKAPKILADIVESTIG 183
           EKLARVAITHGFHRYLRHNK+DL  KIEEFA+ ++ YPLHSNG+IKAPKILADIVESTIG
Sbjct: 122 EKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIG 181

Query: 184 AVFADSNSIDTVWTIFKELLEPLITVKTMKRHPMTELTEICQKRSLKLEFVDLWEETKEI 243
           AVF DSNS+DTVWTIF+ L+EPLIT+KT+KRHPMTELTEICQKR+LKLEFVD WE+TKE+
Sbjct: 182 AVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEV 241

Query: 244 QVFIDKNLWEKEAIAKNL 252
           QVFIDK L  K +  K L
Sbjct: 242 QVFIDKQLVGKGSYRKKL 252

[Close]

BLAST of Lag0014931 vs. ExPASy TrEMBL
Match: A0A6J1GKQ4 (ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.6e-98
Identity = 185/228 (81.14%), Postives = 201/228 (88.16%), Query Frame = 0


Query: 24  MRTESSSSSPAAPSLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVL 83
           ++ E       A SL+G+EAIL YEFRDKRLLEEAFTDASYSPENCSS+ERLEYVGDSVL
Sbjct: 11  LKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVL 70

Query: 84  NFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEF 143
           NFL+TREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNKADL  KIEEF
Sbjct: 71  NFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEF 130

Query: 144 AQSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITVKTMK 203
           +++IE YPLHSNG+IKAPKILADIVES IGAVF DSNSIDTVWTIFK L+EPLIT+KTMK
Sbjct: 131 SKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMK 190

Query: 204 RHPMTELTEICQKRSLKLEFVDLWEETKEIQVFIDKNLWEKEAIAKNL 252
           RHPMTELTE+CQKR+LK EFVDLWEE KEI V IDK L  K +  K L
Sbjct: 191 RHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKL 238

[Close]

BLAST of Lag0014931 vs. ExPASy TrEMBL
Match: A0A1Q3BNT8 (Ribonuclease_3 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_13208 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.9e-72
Identity = 141/206 (68.45%), Postives = 169/206 (82.04%), Query Frame = 0


Query: 38  LEGIEAILNYEFRDKRLLEEAFTDASY-SPENCSSYERLEYVGDSVLNFLMTREQYFSNP 97
           LEG+E IL Y+F +K LLEEAFT ASY SP+ C+SYERLE+VGDSVLN L T+EQYF  P
Sbjct: 1   LEGVEEILGYKFGNKSLLEEAFTHASYTSPDQCASYERLEFVGDSVLNLLFTKEQYFLYP 60

Query: 98  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHSNG 157
           NL PG LTRLRAANVDTEKLARV+I HG HRYL H K  L  +I+EF Q+I  YPLHSNG
Sbjct: 61  NLQPGDLTRLRAANVDTEKLARVSIRHGLHRYLCHKKPLLEQQIQEFTQAILDYPLHSNG 120

Query: 158 MIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITVKTMKRHPMTELTEICQ 217
           ++  PK+LADIVESTIGAVF DSN SID VW +FK LLEP+I+V+T+KRHP+TEL E+CQ
Sbjct: 121 LVDPPKVLADIVESTIGAVFIDSNLSIDIVWKVFKGLLEPIISVETLKRHPVTELYEVCQ 180

Query: 218 KRSLKLEFVDLWEETKEIQVFIDKNL 242
           K++LK++FVDLW+E+   +VFID  L
Sbjct: 181 KKNLKVKFVDLWKESTAFEVFIDDQL 206

[Close]

BLAST of Lag0014931 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 226.1 bits (575), Expect = 3.1e-59
Identity = 114/210 (54.29%), Postives = 146/210 (69.52%), Query Frame = 0


Query: 22  EEMRTESSSSSPAAPSLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDS 81
           +  +T  S+       +E +E ILNY+F+DK LL +AFTDASY  +   SYE LE +GDS
Sbjct: 13  DNTKTIGSADPDQLMEIESLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELLGDS 72

Query: 82  VLNFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIE 141
           +LN  +  +     P  +PG LT+LRA NVDTEKLARVA+ H  + YLRH K  L  +I 
Sbjct: 73  ILNMGIIYDFIKLYPKEAPGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQIL 132

Query: 142 EFAQSIEAYPLHSNGMIKAPKILADIVESTIGAVFADSNSIDTVWTIFKELLEPLITVKT 201
           EF +++E YPLHSNG++K PK+LADIVESTIGA+F D NS +TVW + K LLEP+I +  
Sbjct: 133 EFVEAMEKYPLHSNGLLKVPKVLADIVESTIGAIFMDCNSTETVWKVIKPLLEPIIHLDK 192

Query: 202 MKRHPMTELTEICQKRSLKLEFVDLWEETK 232
           MK HPMTEL E+CQKR+LKL   D WEE +
Sbjct: 193 MKSHPMTELNEMCQKRNLKLTSKDTWEENQ 222

[Close]

BLAST of Lag0014931 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 163.7 bits (413), Expect = 1.9e-40
Identity = 91/207 (43.96%), Postives = 126/207 (60.87%), Query Frame = 0


Query: 40   GIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLS 99
            G+E  L YEF++K LL EA T AS      S Y+RLE+VGD+VL+ L+TR  +F+  +L 
Sbjct: 1561 GLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLP 1620

Query: 100  PGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIE------AYPLH 159
            PG LT LRAA V+ E  ARVA+ H  H YLRH  + L  +I EF + ++       +   
Sbjct: 1621 PGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSF 1680

Query: 160  SNGMIKAPKILADIVESTIGAVFADSNSIDT-VWTIFKELLEPLITVKTMKRHPMTELTE 219
              G  KAPK+L DIVES  GA+F DS    T  W +F+ LL+P++T +T+  HP+ EL E
Sbjct: 1681 GLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPMHPVRELQE 1740

Query: 220  ICQKRSLKLEF-VDLWEETKEIQVFID 239
             CQ+++  LE+       T  ++VFID
Sbjct: 1741 RCQQQAEGLEYKASRSGNTATVEVFID 1767

[Close]

BLAST of Lag0014931 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 163.7 bits (413), Expect = 1.9e-40
Identity = 91/207 (43.96%), Postives = 126/207 (60.87%), Query Frame = 0


Query: 40   GIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLS 99
            G+E  L YEF++K LL EA T AS      S Y+RLE+VGD+VL+ L+TR  +F+  +L 
Sbjct: 1562 GLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLP 1621

Query: 100  PGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIE------AYPLH 159
            PG LT LRAA V+ E  ARVA+ H  H YLRH  + L  +I EF + ++       +   
Sbjct: 1622 PGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSF 1681

Query: 160  SNGMIKAPKILADIVESTIGAVFADSNSIDT-VWTIFKELLEPLITVKTMKRHPMTELTE 219
              G  KAPK+L DIVES  GA+F DS    T  W +F+ LL+P++T +T+  HP+ EL E
Sbjct: 1682 GLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPMHPVRELQE 1741

Query: 220  ICQKRSLKLEF-VDLWEETKEIQVFID 239
             CQ+++  LE+       T  ++VFID
Sbjct: 1742 RCQQQAEGLEYKASRSGNTATVEVFID 1768

[Close]

BLAST of Lag0014931 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 154.1 bits (388), Expect = 1.5e-37
Identity = 83/213 (38.97%), Postives = 136/213 (63.85%), Query Frame = 0


Query: 29  SSSSPAAP------SLEGIEAILNYEFRDKRLLEEAFTDASYSPENCSSYERLEYVGDSV 88
           S S+P+ P      S+E +E ILNY+F +K LL+EA T  S +  +  SYERLE++GDS 
Sbjct: 45  SPSAPSVPVSSEMESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSA 104

Query: 89  LNFLMTREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEE 148
           +   ++   Y + P+L P  L+ LRAANV TEKLARV++ HG + +LR N   L  K++E
Sbjct: 105 IGLAISNYLYLTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKE 164

Query: 149 FAQSI---EAYPLHSNGMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLIT 208
           F++++   +   +   G++KAPK+LAD+ ES  GAV+ D N  +  +W IF+ LLEP++T
Sbjct: 165 FSEAVGKEDDLSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVT 224

Query: 209 VKTMKR--HPMTELTEICQKRSLKLEFVDLWEE 230
           +  +++   P++ L ++C K   +++ +  W++
Sbjct: 225 LDDLQKQPQPVSMLFKLCHKHKKRID-IKNWKD 254

[Close]

BLAST of Lag0014931 vs. TAIR 10
Match: AT3G03300.1 (dicer-like 2 )

HSP 1 Score: 136.3 bits (342), Expect = 3.3e-32
Identity = 79/187 (42.25%), Postives = 111/187 (59.36%), Query Frame = 0


Query: 41   IEAILNYEFRDKRLLEEAFTDASY-SPENCSSYERLEYVGDSVLNFLMTREQYFSNPNLS 100
            +E++LNY F DK LL EA T  SY  PE    Y+RLE++GDSVL++L+T+  Y   P LS
Sbjct: 1152 MESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLS 1211

Query: 101  PGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHTKIEEFAQSIEAYPLHS----N 160
            PG LT +R+A+V+ E  A VA+    H+++ +    LH  I       E   L S     
Sbjct: 1212 PGLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWE 1271

Query: 161  GMIKAPKILADIVESTIGAVFADSN-SIDTVWTIFKELLEPLITVKTMKRHPMTELTEIC 220
              I  PK+L D++ES  GA+F DS  + + V+   K LL  +IT +T+K HP+ ELTE+C
Sbjct: 1272 SDISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELC 1331

Query: 221  QKRSLKL 222
            QK   +L
Sbjct: 1332 QKWQFEL 1338

The following BLAST results are available for this feature:

BLAST of Lag0014931 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888348.1	1.2e-100	77.56	ribonuclease 3-like protein 3 [Benincasa hispida]	[more]
XP_004144598.1	2.0e-100	77.91	ribonuclease 3-like protein 3 [Cucumis sativus] >KGN43500.1 hypothetical protein...	[more]
XP_022969213.1	2.2e-99	82.46	ribonuclease 3-like protein 3 [Cucurbita maxima]	[more]
XP_023554575.1	5.0e-99	82.02	ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo]	[more]
KAG6572281.1	6.5e-99	81.58	Endoribonuclease Dicer-like 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0014931 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69KJ0	1.3e-49	44.98	Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0...	[more]
Q9SP32	2.7e-39	43.96	Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV...	[more]
Q8LMR2	5.6e-37	41.43	Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]
Q9LTQ0	2.1e-36	38.97	Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1	[more]
Q6ATG6	2.4e-35	41.36	Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0...	[more]

BLAST of Lag0014931 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K1J8	9.8e-101	77.91	RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 P...	[more]
A0A6J1I0C1	1.1e-99	82.46	ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 S...	[more]
A0A6J1C375	7.0e-99	75.97	ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=...	[more]
A0A6J1GKQ4	1.6e-98	81.14	ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4...	[more]
A0A1Q3BNT8	1.9e-72	68.45	Ribonuclease_3 domain-containing protein (Fragment) OS=Cephalotus follicularis O...	[more]

BLAST of Lag0014931 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15417.1	3.1e-59	54.29	RNAse II-like 1	[more]
AT1G01040.1	1.9e-40	43.96	dicer-like 1	[more]
AT1G01040.2	1.9e-40	43.96	dicer-like 1	[more]
AT3G20420.1	1.5e-37	38.97	RNAse THREE-like protein 2	[more]
AT3G03300.1	3.3e-32	42.25	dicer-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000999	Ribonuclease III domain	SMART	SM00535	riboneu5	coord: 53..202 e-value: 1.3E-32 score: 124.3
IPR000999	Ribonuclease III domain	PFAM	PF00636	Ribonuclease_3	coord: 73..179 e-value: 3.2E-21 score: 75.9
IPR000999	Ribonuclease III domain	PROSITE	PS00517	RNASE_3_1	coord: 73..81
IPR000999	Ribonuclease III domain	PROSITE	PS50142	RNASE_3_2	coord: 38..180 score: 34.398342
IPR000999	Ribonuclease III domain	CDD	cd00593	RIBOc	coord: 53..198 e-value: 1.46375E-39 score: 131.966
IPR036389	Ribonuclease III, endonuclease domain superfamily	GENE3D	1.10.1520.10	Ribonuclease III domain	coord: 35..209 e-value: 4.8E-49 score: 168.6
IPR036389	Ribonuclease III, endonuclease domain superfamily	SUPERFAMILY	69065	RNase III domain-like	coord: 38..199
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..37
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 24..241
None	No IPR available	PANTHER	PTHR14950:SF54	RNASE II-LIKE 1	coord: 24..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0014931.1	Lag0014931.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0006396	RNA processing
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding