Lag0014879 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014879
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionendoribonuclease Dicer homolog 1
Locationchr12: 5504906 .. 5505724 (+)
RNA-Seq ExpressionLag0014879
SyntenyLag0014879
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAGTATATTACTAGAGGTAGCGTATGGATGATTGAATATATTTGTGTTATTATGGTTTTGACACATTAGTGAATTTAATGTGTGCTTTTTAAGTGCAGGTGGTGCACAAAACTCAATTGAATGTTCATTGTTCTGCTCCTGTGCTATTAAGGTCTCAATTCTTTGTTGGGGGAAATAATTTTGTTATCTGTATTTGTTGTAGGACTTGTGTTAAACAATGTAAAAAAAAGACCAGGTACTGTGTATCAAGTTGAGTCAACTGGTGCCATCGTTAGCTTAAATTCTGCTGTTGGTCTTGTCCATTTCTACTGCTCGCAGCTGCCTAGTGACAGGTTCTGTTTTTACATTATACAAATTGTTTTCCCCATTTTTGTAATTTTAGATGATAATTGAATATATATTTTTTATATCTGCTCCCTTTCTACTAGGTATTCCATTCTTCGACCTGAGTTTGTGATGGTGAGGCATGAGAAACCAGGCGGTCCAACTGAGTACTCTTGTAAGCTTCAACTTCCTTGTAATGCACCATTTGAGGAACTTGAAGGTCCAATTTGCAGCTCAATGCGGCTCGCACAACAGGCATGTTATCATGTCATTCAAAAAATTTATTCCAGGTTTATCATTATAGCTTCACTTTAACCATCTCTATTTAGCAGGCTGTTTGTTTGGCTGCTTGCAAAAAGCTCCATGAAATGGGAGCATTTTCTGACATGCTTTGCCAGACAAGGGAAGCGGAGAGGAGAGGGAAAAGGTAGAACAGAATGATGATGGAGATCCGCTTCCTGGACTGCTAGACACAGGAGTTCTATCCTGA

mRNA sequence

ATGTGGAGTATATTACTAGAGGTGGTGCACAAAACTCAATTGAATGTTCATTGTTCTGCTCCTGTGCTATTAAGACCAGGTACTGTGTATCAAGTTGAGTCAACTGGTGCCATCGTTAGCTTAAATTCTGCTGTTGGTCTTGTCCATTTCTACTGCTCGCAGCTGCCTAGTGACAGGTATTCCATTCTTCGACCTGAGTTTGTGATGGTGAGGCATGAGAAACCAGGCGGTCCAACTGAGTACTCTTGTAAGCTTCAACTTCCTTGTAATGCACCATTTGAGGAACTTGAAGACAAGGGAAGCGGAGAGGAGAGGGAAAAGGTAGAACAGAATGATGATGGAGATCCGCTTCCTGGACTGCTAGACACAGGAGTTCTATCCTGA

Coding sequence (CDS)

ATGTGGAGTATATTACTAGAGGTGGTGCACAAAACTCAATTGAATGTTCATTGTTCTGCTCCTGTGCTATTAAGACCAGGTACTGTGTATCAAGTTGAGTCAACTGGTGCCATCGTTAGCTTAAATTCTGCTGTTGGTCTTGTCCATTTCTACTGCTCGCAGCTGCCTAGTGACAGGTATTCCATTCTTCGACCTGAGTTTGTGATGGTGAGGCATGAGAAACCAGGCGGTCCAACTGAGTACTCTTGTAAGCTTCAACTTCCTTGTAATGCACCATTTGAGGAACTTGAAGACAAGGGAAGCGGAGAGGAGAGGGAAAAGGTAGAACAGAATGATGATGGAGATCCGCTTCCTGGACTGCTAGACACAGGAGTTCTATCCTGA

Protein sequence

MWSILLEVVHKTQLNVHCSAPVLLRPGTVYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQLPCNAPFEELEDKGSGEEREKVEQNDDGDPLPGLLDTGVLS
Homology
[Close]
BLAST of Lag0014879 vs. NCBI nr
Match: XP_022148518.1 (endoribonuclease Dicer homolog 1 [Momordica charantia])

HSP 1 Score: 181.4 bits (459), Expect = 4.8e-42
Identity = 93/128 (72.66%), Postives = 94/128 (73.44%), Query Frame = 0 

Query: 26   PGTVYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKL 85
            PGTVYQVESTGA+VSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKL
Sbjct: 903  PGTVYQVESTGAVVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKL 962

Query: 86   QLPCNAPFEELE----------------------------------DKGSGEEREKVEQN 120
            QLPCNAPFEELE                                  DKGSGEEREKVEQN
Sbjct: 963  QLPCNAPFEELEGPTCSSMRLAQQAVCLAACKKLHEMGAFTDMLLPDKGSGEEREKVEQN 1022

[Close]
BLAST of Lag0014879 vs. NCBI nr
Match: XP_008437750.1 (PREDICTED: endoribonuclease Dicer homolog 1 [Cucumis melo] >TYK20748.1 endoribonuclease Dicer-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 179.1 bits (453), Expect = 2.4e-41
Identity = 91/128 (71.09%), Postives = 94/128 (73.44%), Query Frame = 0 

Query: 26   PGTVYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKL 85
            PGTVYQVESTGA+VSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKL
Sbjct: 905  PGTVYQVESTGAVVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKL 964

Query: 86   QLPCNAPFEELE----------------------------------DKGSGEEREKVEQN 120
            QLPCNAPFE+LE                                  DKGSGEE+EKVEQN
Sbjct: 965  QLPCNAPFEDLEGPICSSMRLAQQAVCLAACKKLHEMGAFTDMLLPDKGSGEEKEKVEQN 1024

[Close]
BLAST of Lag0014879 vs. NCBI nr
Match: XP_038883493.1 (endoribonuclease Dicer homolog 1 [Benincasa hispida])

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-40
Identity = 89/128 (69.53%), Postives = 94/128 (73.44%), Query Frame = 0 

Query: 26   PGTVYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKL 85
            PGTVYQVESTGA+VSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRH++PGGPTEYSCKL
Sbjct: 905  PGTVYQVESTGAVVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHDRPGGPTEYSCKL 964

Query: 86   QLPCNAPFEELE----------------------------------DKGSGEEREKVEQN 120
            QLPCNAPFE+LE                                  DKGSGEE+EKVEQN
Sbjct: 965  QLPCNAPFEDLEGPICSSMRLAQQAVCLAACKKLHEMGAFTDMLLPDKGSGEEKEKVEQN 1024

[Close]
BLAST of Lag0014879 vs. NCBI nr
Match: XP_022938349.1 (endoribonuclease Dicer homolog 1 [Cucurbita moschata])

HSP 1 Score: 176.4 bits (446), Expect = 1.6e-40
Identity = 91/127 (71.65%), Postives = 92/127 (72.44%), Query Frame = 0 

Query: 27   GTVYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQ 86
            GTVYQVESTGA+VSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQ
Sbjct: 906  GTVYQVESTGAVVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQ 965

Query: 87   LPCNAPFEELE----------------------------------DKGSGEEREKVEQND 120
            LPCNAPFEELE                                  DKGSGEEREKVEQ D
Sbjct: 966  LPCNAPFEELEGPICSSMRLAQQAVCLAACKKLHEMGAFTDMLLPDKGSGEEREKVEQKD 1025

[Close]
BLAST of Lag0014879 vs. NCBI nr
Match: XP_023539589.1 (endoribonuclease Dicer homolog 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 176.4 bits (446), Expect = 1.6e-40
Identity = 91/127 (71.65%), Postives = 92/127 (72.44%), Query Frame = 0 

Query: 27   GTVYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQ 86
            GTVYQVESTGA+VSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQ
Sbjct: 906  GTVYQVESTGAVVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQ 965

Query: 87   LPCNAPFEELE----------------------------------DKGSGEEREKVEQND 120
            LPCNAPFEELE                                  DKGSGEEREKVEQ D
Sbjct: 966  LPCNAPFEELEGPICSSMRLAQQAVCLAACKKLHEMGAFTDMLLPDKGSGEEREKVEQKD 1025

[Close]
BLAST of Lag0014879 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 9.2e-36
Identity = 78/134 (58.21%), Postives = 90/134 (67.16%), Query Frame = 0 

Query: 20  APVLLRPGTVYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPT 79
           +PV   PG++YQVESTGA+VSLNSAVGL+HFYCSQLPSDRYSIL PEF+M ++EKPGG  
Sbjct: 793 SPVDTSPGSMYQVESTGAVVSLNSAVGLIHFYCSQLPSDRYSILHPEFIMQKYEKPGGSV 852

Query: 80  EYSCKLQLPCNAPFEELE----------------------------------DKGSGEER 120
           EYSCKLQLPCNAPFE+LE                                  D+GSG E 
Sbjct: 853 EYSCKLQLPCNAPFEKLEGPICSSIRLAQQAVCLAACKKLHEMGAFTDTLLPDRGSG-EG 912

[Close]
BLAST of Lag0014879 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 5.9e-35
Identity = 74/128 (57.81%), Postives = 87/128 (67.97%), Query Frame = 0 

Query: 26  PGTVYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKL 85
           PGTVY+VE+TGA+VSLNSAVGLVHFYCSQLP DRY+ILRPEF M +HEKPGG TEYSC+L
Sbjct: 822 PGTVYKVEATGAMVSLNSAVGLVHFYCSQLPGDRYAILRPEFSMEKHEKPGGHTEYSCRL 881

Query: 86  QLPCNAPFEELE----------------------------------DKGSGEEREKVEQN 120
           QLPCNAPFE LE                                  DKGSG++ EK +Q+
Sbjct: 882 QLPCNAPFEILEGPVCSSMRLAQQAVCLAACKKLHEMGAFTDMLLPDKGSGQDAEKADQD 941

[Close]
BLAST of Lag0014879 vs. ExPASy Swiss-Prot
Match: Q10HL3 (Endoribonuclease Dicer homolog 2a OS=Oryza sativa subsp. japonica OX=39947 GN=DCL2A PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.5e-09
Identity = 34/75 (45.33%), Postives = 48/75 (64.00%), Query Frame = 0 

Query: 28  TVYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQL 87
           T Y+VEST AIV+LNS+V L+HF+CS+LPSD Y    P F +   +K  G    +C L L
Sbjct: 553 TYYRVESTRAIVTLNSSVPLIHFFCSKLPSDEYFNPLPRFDI---DKASG----TCTLHL 612

Query: 88  PCNAPFEELEDKGSG 103
           P ++P + +  +G G
Sbjct: 613 PKSSPVQTVNVEGEG 620

[Close]
BLAST of Lag0014879 vs. ExPASy Swiss-Prot
Match: Q3EBC8 (Endoribonuclease Dicer homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At3g03300 PE=1 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 5.2e-07
Identity = 28/75 (37.33%), Postives = 47/75 (62.67%), Query Frame = 0 

Query: 29  VYQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQLP 88
           +++VESTGA V+L+S+V L++ YCS+LPSD Y    P F + + +        SC L LP
Sbjct: 544 LFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKDQG-------SCTLYLP 603

Query: 89  CNAPFEELEDKGSGE 104
            + P +E++ + + +
Sbjct: 604 KSCPVKEVKAEANNK 611

[Close]
BLAST of Lag0014879 vs. ExPASy Swiss-Prot
Match: P34529 (Endoribonuclease dcr-1 OS=Caenorhabditis elegans OX=6239 GN=dcr-1 PE=1 SV=3)

HSP 1 Score: 55.5 bits (132), Expect = 5.2e-07
Identity = 26/65 (40.00%), Postives = 43/65 (66.15%), Query Frame = 0 

Query: 30  YQVESTGAIVSLNSAVGLVHFYCSQLPSDRYSILRPEFVMVRHEKPGGPTEYSCKLQLPC 89
           Y VE TGA + +++A+ L++ YCS+LPSD ++ L P   ++  E+  G T+Y  +L LP 
Sbjct: 557 YVVEKTGATLKMSTAIALINRYCSKLPSDIFTRLVPHNQIIPIEE-NGVTKYCAELLLPI 616

Query: 90  NAPFE 95
           N+P +
Sbjct: 617 NSPIK 620

The following BLAST results are available for this feature:
BLAST of Lag0014879 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022148518.14.8e-4272.66endoribonuclease Dicer homolog 1 [Momordica charantia][more]
XP_008437750.12.4e-4171.09PREDICTED: endoribonuclease Dicer homolog 1 [Cucumis melo] >TYK20748.1 endoribon... [more]
XP_038883493.11.2e-4069.53endoribonuclease Dicer homolog 1 [Benincasa hispida][more]
XP_022938349.11.6e-4071.65endoribonuclease Dicer homolog 1 [Cucurbita moschata][more]
XP_023539589.11.6e-4071.65endoribonuclease Dicer homolog 1 [Cucurbita pepo subsp. pepo][more]
BLAST of Lag0014879 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8LMR29.2e-3658.21Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL... [more]
Q9SP325.9e-3557.81Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV... [more]
Q10HL32.5e-0945.33Endoribonuclease Dicer homolog 2a OS=Oryza sativa subsp. japonica OX=39947 GN=DC... [more]
Q3EBC85.2e-0737.33Endoribonuclease Dicer homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At3g03300 PE... [more]
P345295.2e-0740.00Endoribonuclease dcr-1 OS=Caenorhabditis elegans OX=6239 GN=dcr-1 PE=1 SV=3[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF03368Dicer_dimercoord: 44..98
e-value: 2.9E-9
score: 36.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 95..127
NoneNo IPR availablePANTHERPTHR14950:SF44ENDORIBONUCLEASE DICER HOMOLOG 1coord: 21..97
NoneNo IPR availablePANTHERPTHR14950DICER-RELATEDcoord: 21..97
IPR038248Dicer dimerisation domain superfamilyGENE3D3.30.160.380Dicer dimerisation domaincoord: 39..110
e-value: 8.3E-14
score: 53.4
IPR005034Dicer dimerisation domainPROSITEPS51327DICER_DSRBFcoord: 44..127
score: 12.342844

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014879.1Lag0014879.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051607 defense response to virus
biological_process GO:0006955 immune response
biological_process GO:0030422 production of siRNA involved in RNA interference
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004525 ribonuclease III activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0016891 endoribonuclease activity, producing 5'-phosphomonoesters