Lag0014581 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014581
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNA pseudouridine synthase 4, mitochondrial
Locationchr12: 2312239 .. 2315531 (+)
RNA-Seq ExpressionLag0014581
SyntenyLag0014581
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACTTCCGGCACTCCTCCGTCGTTCTGTCATCCTCCGGAATTTCCCGTCTTCCGCCGCTCGCGGCGGCGAATGCCTCTATTTCTCAATCTTTCATGAGCTGAAGTCCACCTTCGCCTTGACTAACACCATCGGAACTGTTTCCGAACAGAAAAATGAGAAGAAAGAAAGCAACAGATGGTTCACCTTGCCTCCATACACTGCGACTGTAAGCGGCTCGGTTTTAGGTAATCGCATGGTGAACCAAGGAGCTCGAGCCGAAGCAGAAACTTCCACCACTGCAATCACCGCTCTGAAATGGGTGCTTCGCTGCTGCCCGGAGCTGCCGAGGAGCCTCGTGCAGAAGCTGTTTCGATTGAGAAAGGTTCCGTTCTCAATTTCCCTTAATACTCTTTTAAATTCTTTCATTTGGTCAAGCTAGCTGAATTAATGCCTTAAGATTATCAATTGCCTGCCTGTAATTGAATGTTAAAGTTGTAATTAGCATCGTCAGATTTCGAGTTACTTGTTAACTGTAAGGGAAATGCATGTAAGTTTAGTCATCTTCCTCAATTTATGAATCTTTTCCACCTTTCAGGTTCGAAGAGGTTCTTCTATGATTGAAAGTACTGAATGTAATTTGGAAGCTGAAGAACAACTTCGAAGAGTAAGCAATTCTTTTCTGTTACTTATTTATGTGATTTAGAGAAAGATTTGATTGTGTACGGTGCTATGAATTGAAAAAGGTTATTTTCATTCTTACCACATAAAAGAAAACTTCCTTAGTTCGTTACTAACATTTATAAGTTATTGCTAGGTGGCAGCCAAGGACATTCTGAACACAGGTGATCGAATTTTTCTCCCTGTTTCAGTTCAACTATTTCCCAAAGAGAGAGCTCGGGATTATTGCAATAAAGAAGAAGAGAAATTTATACATAGTTTAGTATTATATAAGGTTTTCACTTTTCACTGTTTTTCCCCTTCTTTTGTTGCCCGGGAGAAAGGTGGGGGTTAAGAATTCTTAGTTCCTCCTGCTGTTTGTGTCAGGATCCAGCAATACTTGTTATCAATAAACCTCCTGGCTTGCCTGTACAGGTATGGCAATCAACTTGTACCATCGATTGATCCTTTGAATAATCGTGATCTTCAAGTTTACAAATTTCTAGCAAATAAAAATATTTCTGCAGGGTGGTATTGGCGTCAAAAGAAGTTTAGATGAATTGGCTGCAAGTTGTTTAAGTTATGACTACTCGGAACCACCTCGGCTTGTAAGTTTTTCTTAAAATCATTGATTTTGTTATAAAGACCACAAGCAGCAATTTTTTTTTACTAATTCTTGAGAGTTTTCCTTCTCTATGTTGAATTCAGTTAAAACATTGAACTCTGTGCTTGGTATTAATTAGTGGTGCTTGAACTTGACCTTTAAATGATCAACTTTGACTTTGGTGAGCTTGTTCAACTTCATTAAATAGCATGACATAATTAAGAACTAAACATTTATGGTATGTTTCCAGCTTCATCCTTTCCCTTTGGTGAAGGGATGTCCGTATTTTGAAATCTTTATCTTTTTAAAAACAAAGATGTGGGCTTTGCTTATTGCTAGGTACATAGGCTTGACAGGGACAGCAGTGGTATCTTGATAATGGGAAGGACTTGCACAAGTACTTCAGTTCTCCATTCTATCTTCCGTGAGAAAACTTTTGGGGCATCAAAACATGTATTTTTCTCTGTACACCTGTTGTTTCTTAAACATTATAAAAGTGTGATGGAAACAAACGAAATTAAAGTATTGCAATCATGTAAACGGGCATGGTTAAGTCTTTTGCTCTGAACTAAAAAATAAGGTTAATTCTTTTACTTTTCCATTGCATCAAAGGATGTTGGTAGTGAGAAGAAAATTTTGCAAAAAAGGTATTGGGCACTTGTAATTGGTTGTCCGAGACGTCCAAAGGGATTGATTGCAGCCCCACTAGGAAAGGTTTGTATAAAATTTCCTTCAAACAACTATTTGATAAGTTTTATCTTATGTTGAGTAGCCATACCTTTGCATTTTGCATTGGTTTTTCATTTCTTGTTCCTTGTCTATTGTCTAGAAATTTACATGTCCGCTTTGATGATTTTAGATTGTTTTGGATGATGGAAGATCTGAGAAAATTACGGTCATTGATAATGCTGAATCTATGCCTTTTCAGCAAGCAATTACTACGTACAAAGTGATCCAATCTCACCATGGTTAGCTCTTTGCCCTTTTTACAAGAAGCATTCAACAATTCACGTTTGATTTTTCAAGTGCTAGACATGTTTTTAATCTGAATAGTCAAGACATACTGGAATCATCACTTGAAAATGCATCAAATTGTAGACTTCGTAGGTTTCTTCATTCTTTTTACTTGGGAAGTTTGTATTGGGCACATGAGATCATCTCTTCAGTATCCCGTTCCTAATAAAGCTTGCATCTGATCATTGGAATGGGAAGGGTTTTCTCTTAATTGAGGGGGTAGGGAATGACTTAGAACTCGTATACATTGTTACTTTTTCTTTGGATCTCAGAATGAGAAAAATTGAATACTGCCCAAGTTAGCTAGAAAGTAGCTTTATGCTCAAGTAGTCAGTTATAATTCTACCTGCCTGGTTTTTGAACGAATGTCTCACAAGTAAAAGAAAAAGCACTTTGCACTCTGTTGTTTTGATTACAAGGATCAATCTTTTTTGATAAAGCATTTCTAATTGAACTAAATTAATTCATGTATAACTTGAACGCCCTGTCTTAACTATGATAGAGTAAACATCAGTCTCCGTTGATTTATTATATGGCAATTATGCAGGATTTACTTGGTTGGAGCTGTGTCCCCTCACTGGGAGAAAACATCAGGTACCATCCAGCACTTCATGTCTCCAGCTACTACAAGTATTAGTCTCTTTTTCTACATATTTTTGGCTGAATATACCTTGCATTGCATGTTTTTAATGCAGCTTCGTGTACATTGTGCTGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGATGGCAAGCACATAGAAATTGGAAACCATTTCCTTCTAATTTCCACAATAACCAAGGAAAGAAACAATCCCTTCCTTTTGGTCTTGATTTTGAAAATGGCAGTATTGCAGAAAATAACCCTCGCCTACACCTTCACTGTAAGCAACTGACTTTACCAGATGTTTCTTGCGCTCTGGATAATGATTCTCTAGATCATGACTGCTCAGGATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCAAAGAAGTTGGGATATCATAAATAATTAG

mRNA sequence

ATGGCACTTCCGGCACTCCTCCGTCGTTCTGTCATCCTCCGGAATTTCCCGTCTTCCGCCGCTCGCGGCGGCGAATGCCTCTATTTCTCAATCTTTCATGAGCTGAAGTCCACCTTCGCCTTGACTAACACCATCGGAACTGTTTCCGAACAGAAAAATGAGAAGAAAGAAAGCAACAGATGGTTCACCTTGCCTCCATACACTGCGACTGTAAGCGGCTCGGTTTTAGGTAATCGCATGGTGAACCAAGGAGCTCGAGCCGAAGCAGAAACTTCCACCACTGCAATCACCGCTCTGAAATGGGTGCTTCGCTGCTGCCCGGAGCTGCCGAGGAGCCTCGTGCAGAAGCTGTTTCGATTGAGAAAGGTTCGAAGAGGTTCTTCTATGATTGAAAGTACTGAATGTAATTTGGAAGCTGAAGAACAACTTCGAAGAGTGGCAGCCAAGGACATTCTGAACACAGGTGATCGAATTTTTCTCCCTGTTTCAGTTCAACTATTTCCCAAAGAGAGAGCTCGGGATTATTGCAATAAAGAAGAAGAGAAATTTATACATAGTTTAGTATTATATAAGGATCCAGCAATACTTGTTATCAATAAACCTCCTGGCTTGCCTGTACAGGGTGGTATTGGCGTCAAAAGAAGTTTAGATGAATTGGCTGCAAGTTGTTTAAGTTATGACTACTCGGAACCACCTCGGCTTGTACATAGGCTTGACAGGGACAGCAGTGGTATCTTGATAATGGGAAGGACTTGCACAAGTACTTCAGTTCTCCATTCTATCTTCCGTGAGAAAACTTTTGGGGCATCAAAACATGATGTTGGTAGTGAGAAGAAAATTTTGCAAAAAAGGTATTGGGCACTTGTAATTGGTTGTCCGAGACGTCCAAAGGGATTGATTGCAGCCCCACTAGGAAAGATTGTTTTGGATGATGGAAGATCTGAGAAAATTACGGTCATTGATAATGCTGAATCTATGCCTTTTCAGCAAGCAATTACTACGTACAAAGTGATCCAATCTCACCATGGATTTACTTGGTTGGAGCTGTGTCCCCTCACTGGGAGAAAACATCAGCTTCGTGTACATTGTGCTGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGATGGCAAGCACATAGAAATTGGAAACCATTTCCTTCTAATTTCCACAATAACCAAGGAAAGAAACAATCCCTTCCTTTTGGTCTTGATTTTGAAAATGGCAGTATTGCAGAAAATAACCCTCGCCTACACCTTCACTGTAAGCAACTGACTTTACCAGATGTTTCTTGCGCTCTGGATAATGATTCTCTAGATCATGACTGCTCAGGATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCAAAGAAGTTGGGATATCATAAATAATTAG

Coding sequence (CDS)

ATGGCACTTCCGGCACTCCTCCGTCGTTCTGTCATCCTCCGGAATTTCCCGTCTTCCGCCGCTCGCGGCGGCGAATGCCTCTATTTCTCAATCTTTCATGAGCTGAAGTCCACCTTCGCCTTGACTAACACCATCGGAACTGTTTCCGAACAGAAAAATGAGAAGAAAGAAAGCAACAGATGGTTCACCTTGCCTCCATACACTGCGACTGTAAGCGGCTCGGTTTTAGGTAATCGCATGGTGAACCAAGGAGCTCGAGCCGAAGCAGAAACTTCCACCACTGCAATCACCGCTCTGAAATGGGTGCTTCGCTGCTGCCCGGAGCTGCCGAGGAGCCTCGTGCAGAAGCTGTTTCGATTGAGAAAGGTTCGAAGAGGTTCTTCTATGATTGAAAGTACTGAATGTAATTTGGAAGCTGAAGAACAACTTCGAAGAGTGGCAGCCAAGGACATTCTGAACACAGGTGATCGAATTTTTCTCCCTGTTTCAGTTCAACTATTTCCCAAAGAGAGAGCTCGGGATTATTGCAATAAAGAAGAAGAGAAATTTATACATAGTTTAGTATTATATAAGGATCCAGCAATACTTGTTATCAATAAACCTCCTGGCTTGCCTGTACAGGGTGGTATTGGCGTCAAAAGAAGTTTAGATGAATTGGCTGCAAGTTGTTTAAGTTATGACTACTCGGAACCACCTCGGCTTGTACATAGGCTTGACAGGGACAGCAGTGGTATCTTGATAATGGGAAGGACTTGCACAAGTACTTCAGTTCTCCATTCTATCTTCCGTGAGAAAACTTTTGGGGCATCAAAACATGATGTTGGTAGTGAGAAGAAAATTTTGCAAAAAAGGTATTGGGCACTTGTAATTGGTTGTCCGAGACGTCCAAAGGGATTGATTGCAGCCCCACTAGGAAAGATTGTTTTGGATGATGGAAGATCTGAGAAAATTACGGTCATTGATAATGCTGAATCTATGCCTTTTCAGCAAGCAATTACTACGTACAAAGTGATCCAATCTCACCATGGATTTACTTGGTTGGAGCTGTGTCCCCTCACTGGGAGAAAACATCAGCTTCGTGTACATTGTGCTGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGATGGCAAGCACATAGAAATTGGAAACCATTTCCTTCTAATTTCCACAATAACCAAGGAAAGAAACAATCCCTTCCTTTTGGTCTTGATTTTGAAAATGGCAGTATTGCAGAAAATAACCCTCGCCTACACCTTCACTGTAAGCAACTGACTTTACCAGATGTTTCTTGCGCTCTGGATAATGATTCTCTAGATCATGACTGCTCAGGATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCAAAGAAGTTGGGATATCATAAATAATTAG

Protein sequence

MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNRWFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRLRKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRDSSGILIMGRTCTSTSVLHSIFREKTFGASKHDVGSEKKILQKRYWALVIGCPRRPKGLIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNPRLHLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN
Homology
[Close]
BLAST of Lag0014581 vs. NCBI nr
Match: XP_022148845.1 (RNA pseudouridine synthase 4, mitochondrial [Momordica charantia])

HSP 1 Score: 855.5 bits (2209), Expect = 2.1e-244
Identity = 409/470 (87.02%), Postives = 440/470 (93.62%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M LPALLRRS+IL   PSS ARGG  L+FS+FHELKSTFAL+NT  T+SE+KNEKK  NR
Sbjct: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPPYTATV GSVLGNR++NQG++AE ETSTTAITALKWVLRCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RK+RRGSSMIES+ECNLEA+ QL+RVAAKDILN GD+IFLP+SVQ+FP+ERARDYCNKEE
Sbjct: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           EKF+HSLVLYKDPAI+VINKPPGLPVQGGIG+ RSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKHDVGSEKKILQKRYWALVIGCPRRPKGLI 300
           DSSGIL+MGRT TSTSVLHSIFREKTFGASKH V SEKKILQK+YWALVIG PRRP GLI
Sbjct: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRRPNGLI 300

Query: 301 AAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQLR 360
            APLGKIVLDDGRSEKI VIDNAESM FQQAITTY+VI+SHHG+TWLELCPLTGRKHQLR
Sbjct: 301 TAPLGKIVLDDGRSEKIMVIDNAESMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQLR 360

Query: 361 VHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNPRL 420
           VHCAEVLGTPIVGDYKYGWQAHRNW+PFPSNFH NQGKKQSLPFGLDFENGS+AEN+PRL
Sbjct: 361 VHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENSPRL 420

Query: 421 HLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           HLHCKQL LP+VS AL NDSLDHD S LECLELDAPLPSYM+RSWDIINN
Sbjct: 421 HLHCKQLALPNVSGALPNDSLDHDFSELECLELDAPLPSYMRRSWDIINN 470

[Close]
BLAST of Lag0014581 vs. NCBI nr
Match: XP_023553811.1 (RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 824.3 bits (2128), Expect = 5.3e-235
Identity = 405/472 (85.81%), Postives = 430/472 (91.10%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M LPA+LRRSVI R+FPSSAA GGE L+FSIF E KSTFAL+N   TVSEQKNE K SNR
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TATV+GS LGNR++N GA+AEAETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATVNGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RKVRRGSSMIE++ECNLEA +QL+RVAA DILN GD++ LP+SVQ+FP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKH--DVGSEKKILQKRYWALVIGCPRRPKG 300
           DSSGILIMGRT TSTSVLHSIFREKT GASKH   V  EKKILQKRYWALVIG PRR KG
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKKILQKRYWALVIGSPRRTKG 300

Query: 301 LIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQ 360
           LI APL K+VLDDGRSE+I V+DNAESMPFQ AITTYKVI++HHG+TWLELCPLTGRKHQ
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVVDNAESMPFQHAITTYKVIKTHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFP N H NQGKKQSLPFGLDFE+GSIAENNP
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENNP 420

Query: 421 RLHLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           RLHLHCK LTLPDVSCAL NDSLDHDCS LE LELDAPLPSYMQRSWDIINN
Sbjct: 421 RLHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]
BLAST of Lag0014581 vs. NCBI nr
Match: KAG7011636.1 (RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 821.6 bits (2121), Expect = 3.4e-234
Identity = 404/472 (85.59%), Postives = 430/472 (91.10%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M  PA+LRRSVI R+FPSSAA GGE L+FSIF E KSTFAL++   TVSEQKNE K SNR
Sbjct: 1   MVFPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSSATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TAT +GS LGNR++N GA+AEAETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RKVRRGSSMIE++ECNLEA +QL+RVAA DILN GD++ LP+SVQ+FP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKH--DVGSEKKILQKRYWALVIGCPRRPKG 300
           DSSGILIMGRT TSTSVLHSIFREKT GASKH   V SEKKILQKRYWALVIG PRR KG
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKG 300

Query: 301 LIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQ 360
           LI APL K+VLDDGRSE+I VIDNAESMPFQ AITTYKVI+SHHG+TWLELCPLTGRKHQ
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFP N H NQGKKQSLPFGLDFE+GSIAEN+P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSP 420

Query: 421 RLHLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           RLHLHCKQLTLPDVSCAL NDSLDHDCS LE LELDAPLPSYMQRSWDIINN
Sbjct: 421 RLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]
BLAST of Lag0014581 vs. NCBI nr
Match: XP_022952780.1 (RNA pseudouridine synthase 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 820.5 bits (2118), Expect = 7.6e-234
Identity = 404/472 (85.59%), Postives = 430/472 (91.10%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M LPA+LRRSVI R+FPSSAA GGE L+FSIF E KST+AL+    TVSEQKNE K SNR
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTYALSIATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TAT +GS LGNR++N GA+AEAETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RKVRRGSSMIE++ECNLEA +QL+RVAA DILN GD++ LP+SVQ+FP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKH--DVGSEKKILQKRYWALVIGCPRRPKG 300
           DSSGILIMGRT TSTSVLHSIFREKT GASKH   V SEKKILQKRYWALVIG PRR KG
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKG 300

Query: 301 LIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQ 360
           LI APL K+VLDDGRSE+I VIDNAESMPFQ AITTYKVI+SHHG+TWLELCPLTGRKHQ
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFP N H NQGKKQSLPFGLDFE+GSIAEN+P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSP 420

Query: 421 RLHLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           RLHLHCKQLTLPDVSCAL NDSLDHDCS LE LELDAPLPSYMQRSWDIINN
Sbjct: 421 RLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]
BLAST of Lag0014581 vs. NCBI nr
Match: KAG6571947.1 (RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 818.5 bits (2113), Expect = 2.9e-233
Identity = 403/472 (85.38%), Postives = 429/472 (90.89%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M  PA+LRRSVI R+FPSSAA GGE L+FSIF E KSTFAL++   TVSEQKNE K SNR
Sbjct: 1   MVFPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSSATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TAT +GS LGNR++N GA+AEAETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RKVRRGSSMIE++ECNLEA +QL+RVAA DILN GD++ LP+SVQ+FP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKH--DVGSEKKILQKRYWALVIGCPRRPKG 300
           DSSGILIMGRT TSTSVLHSIFREKT GASKH   V SEKKILQKRYWALVIG PRR KG
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKG 300

Query: 301 LIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQ 360
           LI APL K+VLDDGRSE+I VIDNAESMPFQ AITTYKVI+SHHG+TWLELCPLTGRKHQ
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFP N H NQGKKQSLPFGLDFE+GSIAEN+P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSP 420

Query: 421 RLHLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           RLHLHCKQLTLPDVSCAL NDSLDHDCS LE LELDAPLPSYMQRSWDII N
Sbjct: 421 RLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIIYN 471

[Close]
BLAST of Lag0014581 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 1.6e-133
Identity = 239/419 (57.04%), Postives = 309/419 (73.75%), Query Frame = 0 

Query: 60  RWFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFR 119
           +W TLPP++ T+ G+ +G  +++ G   ++ T  +  TAL+W+LRC P+LPR+LVQKLFR
Sbjct: 54  KWLTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFR 113

Query: 120 LRKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSV-----QLFPKERARD 179
           LR+VRR  SM  S + +     QL+RVAAK+ LN GDRI+LP+SV     Q  P ++   
Sbjct: 114 LRQVRREMSM--SVDGDELQRSQLKRVAAKESLNVGDRIYLPLSVDNDTPQTPPAKKESF 173

Query: 180 YCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRL 239
            C+ EE KF+ SLVLYKDPAI+V+NKP GL VQGG G+K S+DELAASCL +D SE PRL
Sbjct: 174 QCSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRL 233

Query: 240 VHRLDRDSSGILIMGRTCTSTSVLHSIFREKTFGASKHDVGSEKKILQKRYWALVIGCPR 299
           VHRLDRD SG+L++ RT T+ +VLHSIFREKT GAS + V    K L+++Y ALVIGCP 
Sbjct: 234 VHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGVKKNVKSLKRKYMALVIGCPP 293

Query: 300 RPKGLIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSH-HGFTWLELCPLT 359
           R +G I+APL K+V+DDG+SE+ITV DN E +  Q AIT Y+VI+S  HG+TWLEL PLT
Sbjct: 294 RQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLT 353

Query: 360 GRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSI 419
           GRKHQLRVHCAEVLGTPIVGDYKYGWQAH+  +PF S+  NN  K+ S PFGLD + G +
Sbjct: 354 GRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS-ENNPTKQSSSPFGLDLDGGDV 413

Query: 420 AENNPRLHLHCKQLTLPDVSCALD--NDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           +   P LHLH KQ+ LP++S  L+    S D D S L+ L+ DAPLPS+MQ S++++ +
Sbjct: 414 SSKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLDSLKFDAPLPSHMQLSFNLLKS 469

[Close]
BLAST of Lag0014581 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.7e-79
Identity = 167/429 (38.93%), Postives = 249/429 (58.04%), Query Frame = 0 

Query: 63  TLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRLRK 122
           +LPP+     G  L  R      R         +TA+ WV     ++P+ +VQ  F  R 
Sbjct: 55  SLPPFPGGGDGDPLPGRKAAAPRR---------VTAVSWVKHYLADVPQEVVQAHFNKRL 114

Query: 123 VRRGSSMIE-STECNLEAEEQLRRVAAKDILNTGDRIFLPVSV---------QLFPKERA 182
           V    S  E S E     +  L+++   D++  G RI LPVSV         +  P   A
Sbjct: 115 VYSECSDHEVSVETIKSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIP--TA 174

Query: 183 RDYCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPP 242
             + NK+E +++  LV++KD AILV+NKPP +P++G + V  S+D LAA+ LSY   E P
Sbjct: 175 TLHPNKDEIEYLRRLVIHKDSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGP 234

Query: 243 RLVHRLDRDSSGILIMGRTCTSTSVLHSIFREKTFGASKHDV-GSEKKILQKRYWALVIG 302
           +LVHRLDR+SSG+L+ GRT  S + LH +F       +   V  +  +   +RYWALVIG
Sbjct: 235 KLVHRLDRESSGLLLFGRTKESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIG 294

Query: 303 CPRRPKGLIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVI-QSHHGFTWLELC 362
            P+  +G+I+APL K++LDDG++E++ +   +     Q+A+T Y+V+  + HG +W+EL 
Sbjct: 295 TPKEREGIISAPLSKVLLDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELR 354

Query: 363 PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPS-NFHNNQGKKQSL--PFGLD 422
           PLTGRKHQLRVHCAE LGTPIVGDYKYGW  H+ WK  P  +F    G+   L  P GL+
Sbjct: 355 PLTGRKHQLRVHCAEALGTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLE 414

Query: 423 FENGSIAENNPRLHLHCKQLTLPDVSCALDNDSLDHDCSG------LECLELDAPLPSYM 471
            + GS+    P LHLHC+++ +P+++  L ++   H+            L   AP+P++M
Sbjct: 415 IQKGSVLSKVPLLHLHCREMVIPNIAKFLSSNGEWHENGAPWSKEKPNLLRFIAPMPAHM 472

[Close]
BLAST of Lag0014581 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 3.0e-76
Identity = 167/462 (36.15%), Postives = 259/462 (56.06%), Query Frame = 0 

Query: 37  STFALTNTIGTVSEQKNEKKESNRWFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAI 96
           S   +TN +  +   K +     +  +LPP+     G  L  +  N GA  +   S   +
Sbjct: 29  SVIRVTNNVAHLGPPK-QGPLPRQLISLPPF----PGHPLPGK--NAGADGDDGDSGGHV 88

Query: 97  TALKWVLRCCPEL---------PRSLVQKLFRLRKVRRGSSMIESTECNLEAEEQLRRVA 156
           TA+ WV     E+          + LVQ  FR R+        +++    +    +R++ 
Sbjct: 89  TAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRR--------DASREKEDGAIPMRKIK 148

Query: 157 AKDILNTGDRIFLPVSVQLFPKERARD-------YCNKEEEKFIHSLVLYKDPAILVINK 216
             +++  GD+I+LPVS+      +  D       Y N +E  ++  LV +KD AI+V+NK
Sbjct: 149 HNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDSAIIVLNK 208

Query: 217 PPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRDSSGILIMGRTCTSTSVLHS 276
           PP LPV+G + +  S+D LAA+ LS+   E PRLVHRLDR++SG+L+MGRT  S   LHS
Sbjct: 209 PPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKESIDYLHS 268

Query: 277 IFRE-KTFGASKHDVGSEKKILQKRYWALVIGCPRRPKGLIAAPLGKIVLDDGRSEKITV 336
           +F + K   +S        + + ++YWALVIG P+  +GLI+APL K++LDDG+++++ +
Sbjct: 269 VFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLDDGKTDRVVL 328

Query: 337 IDNAESMPFQQAITTYKVI-QSHHGFTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 396
              +     Q AIT YKV+    +G +W+EL P+T RKHQLRVHCAE LGTPIVGDYKYG
Sbjct: 329 AQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPIVGDYKYG 388

Query: 397 WQAHRNWKPFPS-NFHNNQGKKQSL--PFGLDFENGSIAENNPRLHLHCKQLTLPDVS-- 456
           W  H+ WK  P  +     GK   L  P GLD + GS+    P LHLHC+++ LP+++  
Sbjct: 389 WFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMVLPNIAKF 448

Query: 457 -CALDNDSLDHDCSGL----ECLELDAPLPSYMQRSWDIINN 471
              ++    +   +G+    + L   A +PS+M+ SW+++++
Sbjct: 449 LHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

[Close]
BLAST of Lag0014581 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 7.5e-75
Identity = 150/301 (49.83%), Postives = 204/301 (67.77%), Query Frame = 0 

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           W  LPP+    + +    R +++G   E        TA+KWV RCCP+LP SLVQKLFRL
Sbjct: 52  WLQLPPFAPLDAAAAA--RAISRGG-GEGGDGEQGATAIKWVRRCCPDLPTSLVQKLFRL 111

Query: 121 RKVRRGSSMIESTECNLEAEE-QLRRVAAKDILNTGDRIFLPVSV-QLFPKERARDYCNK 180
           RKV++     E +  +  AE+ +LRRV+AKD L  GD +FLPV++ +    E+ + + N+
Sbjct: 112 RKVKKNVVTAEISSADASAEQHRLRRVSAKDQLMPGDILFLPVNLKESSVAEKTKKFDNR 171

Query: 181 EEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRL 240
            E  F+  L +YKD AI+V+NKPPG+PVQGG+G+K S+D L AS    + SE PRLVHRL
Sbjct: 172 NEINFLRGLEIYKDEAIIVVNKPPGMPVQGGVGIKNSIDVL-ASMFEENSSEAPRLVHRL 231

Query: 241 DRDSSGILIMGRTCTSTSVLHSIFREKTFGASKHDVGSEKKILQKRYWALVIGCPRRPKG 300
           DRD SGIL++GR   STS+LH+IFREKT  A        + +LQ++Y ALVIG PR PKG
Sbjct: 232 DRDCSGILVLGRNQLSTSMLHAIFREKTADAL---ADGTQHVLQRKYVALVIGTPRHPKG 291

Query: 301 LIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQS-HHGFTWLELCPLTGRKH 359
           L++APL KI+L DG+SE++T+  ++ +   Q A+T Y+VI+S   G+TWLEL P TGRKH
Sbjct: 292 LLSAPLAKILLQDGKSERLTIRASSNAASVQDALTEYRVIESCPQGYTWLELFPRTGRKH 345

[Close]
BLAST of Lag0014581 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.0e-28
Identity = 89/283 (31.45%), Postives = 148/283 (52.30%), Query Frame = 0 

Query: 100 KWVLRCCPELPRSLVQKLFRLRKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIF 159
           K++ R  P L + +++K  R ++V            N +  E   RV   D +   D   
Sbjct: 15  KYLKRLYPSLTQGVIEKALRQKQV----------TVNFQKAEANLRVKEGDTIFINDYFN 74

Query: 160 LPVSVQLFPKERARDYCNKE---EEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSL 219
           LPV+     +     + + E    +K +   ++Y+D  ++ INKP  L  QGG  +  S+
Sbjct: 75  LPVT-----QHETLVFADAEIKLAKKILTDYLIYEDDHLIAINKPASLATQGGSKINLSI 134

Query: 220 DELAASCLSYDYSEPPRLVHRLDRDSSGILIMGRTCTSTSVLHSIFREKTFGASKHDVGS 279
           D  A   L+Y  ++  +LVHRLD+++SG+L++ +   S+  LH  F+E            
Sbjct: 135 DS-ALKYLNYQGAD-FKLVHRLDKETSGLLLIAKNYLSSVKLHDAFKE------------ 194

Query: 280 EKKILQKRYWALVIGCPRRPKGLIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYK 339
             K++ K+Y+A+  G P +  G++ + +GK     GR  KIT ID+      + AIT YK
Sbjct: 195 --KLVIKKYFAITYGRPVKNVGIVKSNIGK---SKGRMFKITDIDSDNG---KLAITYYK 254

Query: 340 VIQS-HHGFTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 379
           +++S ++    +E  P+TGR HQLR+H A++LG PI+GD KYG
Sbjct: 255 LLKSLNNNLFLIEFMPVTGRMHQLRLH-AQLLGCPILGDDKYG 259

[Close]
BLAST of Lag0014581 vs. ExPASy TrEMBL
Match: A0A6J1D432 (RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017405 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.0e-244
Identity = 409/470 (87.02%), Postives = 440/470 (93.62%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M LPALLRRS+IL   PSS ARGG  L+FS+FHELKSTFAL+NT  T+SE+KNEKK  NR
Sbjct: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPPYTATV GSVLGNR++NQG++AE ETSTTAITALKWVLRCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RK+RRGSSMIES+ECNLEA+ QL+RVAAKDILN GD+IFLP+SVQ+FP+ERARDYCNKEE
Sbjct: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           EKF+HSLVLYKDPAI+VINKPPGLPVQGGIG+ RSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKHDVGSEKKILQKRYWALVIGCPRRPKGLI 300
           DSSGIL+MGRT TSTSVLHSIFREKTFGASKH V SEKKILQK+YWALVIG PRRP GLI
Sbjct: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRRPNGLI 300

Query: 301 AAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQLR 360
            APLGKIVLDDGRSEKI VIDNAESM FQQAITTY+VI+SHHG+TWLELCPLTGRKHQLR
Sbjct: 301 TAPLGKIVLDDGRSEKIMVIDNAESMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQLR 360

Query: 361 VHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNPRL 420
           VHCAEVLGTPIVGDYKYGWQAHRNW+PFPSNFH NQGKKQSLPFGLDFENGS+AEN+PRL
Sbjct: 361 VHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENSPRL 420

Query: 421 HLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           HLHCKQL LP+VS AL NDSLDHD S LECLELDAPLPSYM+RSWDIINN
Sbjct: 421 HLHCKQLALPNVSGALPNDSLDHDFSELECLELDAPLPSYMRRSWDIINN 470

[Close]
BLAST of Lag0014581 vs. ExPASy TrEMBL
Match: A0A6J1GMP9 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455375 PE=4 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.7e-234
Identity = 404/472 (85.59%), Postives = 430/472 (91.10%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M LPA+LRRSVI R+FPSSAA GGE L+FSIF E KST+AL+    TVSEQKNE K SNR
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTYALSIATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TAT +GS LGNR++N GA+AEAETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RKVRRGSSMIE++ECNLEA +QL+RVAA DILN GD++ LP+SVQ+FP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKH--DVGSEKKILQKRYWALVIGCPRRPKG 300
           DSSGILIMGRT TSTSVLHSIFREKT GASKH   V SEKKILQKRYWALVIG PRR KG
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKG 300

Query: 301 LIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQ 360
           LI APL K+VLDDGRSE+I VIDNAESMPFQ AITTYKVI+SHHG+TWLELCPLTGRKHQ
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFP N H NQGKKQSLPFGLDFE+GSIAEN+P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSP 420

Query: 421 RLHLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           RLHLHCKQLTLPDVSCAL NDSLDHDCS LE LELDAPLPSYMQRSWDIINN
Sbjct: 421 RLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]
BLAST of Lag0014581 vs. ExPASy TrEMBL
Match: A0A6J1IBC5 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111470950 PE=4 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 5.5e-230
Identity = 400/473 (84.57%), Postives = 425/473 (89.85%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M LPALLRRSVI R+FPSSAA GGE L+FSIF E KSTFAL+N   TVSEQKNE K SNR
Sbjct: 1   MVLPALLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TATV+GS LGNR++N GA++EAETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATVNGSALGNRIMNGGAQSEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RKVRRGSSMIE++ECNLEA +QL+RVAA DILN GD++ LP+SVQ+FP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKH--DVGSEKKILQKRYWALVIGCPRRPKG 300
           DSSGILIMGRT TSTSVLHSIFREKT GASKH   V  EK ILQKRYWALVIG P   KG
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKMILQKRYWALVIGSPSHTKG 300

Query: 301 LIAAPLGKIVLDDGRSEKITVIDNAES-MPFQQAITTYKVIQSHHGFTWLELCPLTGRKH 360
           LI APL K+VLDDGRSE+I V+DNAES MPFQ AITTYKVI+SHHG+TW+ELCPLTGRKH
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVVDNAESIMPFQHAITTYKVIKSHHGYTWVELCPLTGRKH 360

Query: 361 QLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENN 420
           QLRVHCAE LGTPIVGDYKYGWQAHRN KPFP N H NQGKKQSLPFGLDFE+GSIAENN
Sbjct: 361 QLRVHCAEALGTPIVGDYKYGWQAHRNLKPFPHNSHKNQGKKQSLPFGLDFESGSIAENN 420

Query: 421 PRLHLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           PRLHLHCK LTLPDVSCAL NDSLDHDCS LE LELDAPLPSYMQRSWDIINN
Sbjct: 421 PRLHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 472

[Close]
BLAST of Lag0014581 vs. ExPASy TrEMBL
Match: A0A5D3C4F5 (RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00930 PE=4 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.4e-225
Identity = 388/472 (82.20%), Postives = 416/472 (88.14%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M LP L R SVILR  PS      E LYFSIFH LKST AL+N    VS+QKNE K SNR
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNE-KGSNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPPYTATV+ S+LGNR++N+GA+AE ETST AITALKWV+ CCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RKVRRGSSMIE++E NLEA +QL+RVAAKDILN GD+IFLP+S Q+F  E+ RDYCNK+E
Sbjct: 121 RKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           E F+HSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDR
Sbjct: 181 ETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKH--DVGSEKKILQKRYWALVIGCPRRPKG 300
           DSSGILIMGRT TSTS+LHS+FREKTF ASKH   V SEKKIL KRYWALVIG PRR KG
Sbjct: 241 DSSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKG 300

Query: 301 LIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQ 360
           LI APLGKIVLDDGRSE+I VIDNAE MPFQQAIT YKVI+SHHG+TWLELCPLTGRKHQ
Sbjct: 301 LITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNP 420
           LRVHCAEVLGTPIVGDYKYGWQAH+NWKPF  NFH NQGKKQ LPFGLDFE+GSIAE NP
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNP 420

Query: 421 RLHLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           RLHLHCKQLTLPDVS AL NDSLD DCS LECLELDAPLPSYMQ+SWDIIN+
Sbjct: 421 RLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]
BLAST of Lag0014581 vs. ExPASy TrEMBL
Match: A0A1S3BZE7 (RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495275 PE=4 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.4e-225
Identity = 388/472 (82.20%), Postives = 416/472 (88.14%), Query Frame = 0 

Query: 1   MALPALLRRSVILRNFPSSAARGGECLYFSIFHELKSTFALTNTIGTVSEQKNEKKESNR 60
           M LP L R SVILR  PS      E LYFSIFH LKST AL+N    VS+QKNE K SNR
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNE-KGSNR 60

Query: 61  WFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPPYTATV+ S+LGNR++N+GA+AE ETST AITALKWV+ CCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEE 180
           RKVRRGSSMIE++E NLEA +QL+RVAAKDILN GD+IFLP+S Q+F  E+ RDYCNK+E
Sbjct: 121 RKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDE 180

Query: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
           E F+HSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDR
Sbjct: 181 ETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTCTSTSVLHSIFREKTFGASKH--DVGSEKKILQKRYWALVIGCPRRPKG 300
           DSSGILIMGRT TSTS+LHS+FREKTF ASKH   V SEKKIL KRYWALVIG PRR KG
Sbjct: 241 DSSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKG 300

Query: 301 LIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSHHGFTWLELCPLTGRKHQ 360
           LI APLGKIVLDDGRSE+I VIDNAE MPFQQAIT YKVI+SHHG+TWLELCPLTGRKHQ
Sbjct: 301 LITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSIAENNP 420
           LRVHCAEVLGTPIVGDYKYGWQAH+NWKPF  NFH NQGKKQ LPFGLDFE+GSIAE NP
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNP 420

Query: 421 RLHLHCKQLTLPDVSCALDNDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           RLHLHCKQLTLPDVS AL NDSLD DCS LECLELDAPLPSYMQ+SWDIIN+
Sbjct: 421 RLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]
BLAST of Lag0014581 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 477.6 bits (1228), Expect = 1.1e-134
Identity = 239/419 (57.04%), Postives = 309/419 (73.75%), Query Frame = 0 

Query: 60  RWFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAITALKWVLRCCPELPRSLVQKLFR 119
           +W TLPP++ T+ G+ +G  +++ G   ++ T  +  TAL+W+LRC P+LPR+LVQKLFR
Sbjct: 54  KWLTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFR 113

Query: 120 LRKVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSV-----QLFPKERARD 179
           LR+VRR  SM  S + +     QL+RVAAK+ LN GDRI+LP+SV     Q  P ++   
Sbjct: 114 LRQVRREMSM--SVDGDELQRSQLKRVAAKESLNVGDRIYLPLSVDNDTPQTPPAKKESF 173

Query: 180 YCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRL 239
            C+ EE KF+ SLVLYKDPAI+V+NKP GL VQGG G+K S+DELAASCL +D SE PRL
Sbjct: 174 QCSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRL 233

Query: 240 VHRLDRDSSGILIMGRTCTSTSVLHSIFREKTFGASKHDVGSEKKILQKRYWALVIGCPR 299
           VHRLDRD SG+L++ RT T+ +VLHSIFREKT GAS + V    K L+++Y ALVIGCP 
Sbjct: 234 VHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGVKKNVKSLKRKYMALVIGCPP 293

Query: 300 RPKGLIAAPLGKIVLDDGRSEKITVIDNAESMPFQQAITTYKVIQSH-HGFTWLELCPLT 359
           R +G I+APL K+V+DDG+SE+ITV DN E +  Q AIT Y+VI+S  HG+TWLEL PLT
Sbjct: 294 RQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLT 353

Query: 360 GRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPSNFHNNQGKKQSLPFGLDFENGSI 419
           GRKHQLRVHCAEVLGTPIVGDYKYGWQAH+  +PF S+  NN  K+ S PFGLD + G +
Sbjct: 354 GRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS-ENNPTKQSSSPFGLDLDGGDV 413

Query: 420 AENNPRLHLHCKQLTLPDVSCALD--NDSLDHDCSGLECLELDAPLPSYMQRSWDIINN 471
           +   P LHLH KQ+ LP++S  L+    S D D S L+ L+ DAPLPS+MQ S++++ +
Sbjct: 414 SSKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLDSLKFDAPLPSHMQLSFNLLKS 469

[Close]
BLAST of Lag0014581 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 287.3 bits (734), Expect = 2.2e-77
Identity = 167/462 (36.15%), Postives = 259/462 (56.06%), Query Frame = 0 

Query: 37  STFALTNTIGTVSEQKNEKKESNRWFTLPPYTATVSGSVLGNRMVNQGARAEAETSTTAI 96
           S   +TN +  +   K +     +  +LPP+     G  L  +  N GA  +   S   +
Sbjct: 29  SVIRVTNNVAHLGPPK-QGPLPRQLISLPPF----PGHPLPGK--NAGADGDDGDSGGHV 88

Query: 97  TALKWVLRCCPEL---------PRSLVQKLFRLRKVRRGSSMIESTECNLEAEEQLRRVA 156
           TA+ WV     E+          + LVQ  FR R+        +++    +    +R++ 
Sbjct: 89  TAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRR--------DASREKEDGAIPMRKIK 148

Query: 157 AKDILNTGDRIFLPVSVQLFPKERARD-------YCNKEEEKFIHSLVLYKDPAILVINK 216
             +++  GD+I+LPVS+      +  D       Y N +E  ++  LV +KD AI+V+NK
Sbjct: 149 HNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDSAIIVLNK 208

Query: 217 PPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRDSSGILIMGRTCTSTSVLHS 276
           PP LPV+G + +  S+D LAA+ LS+   E PRLVHRLDR++SG+L+MGRT  S   LHS
Sbjct: 209 PPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKESIDYLHS 268

Query: 277 IFRE-KTFGASKHDVGSEKKILQKRYWALVIGCPRRPKGLIAAPLGKIVLDDGRSEKITV 336
           +F + K   +S        + + ++YWALVIG P+  +GLI+APL K++LDDG+++++ +
Sbjct: 269 VFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLDDGKTDRVVL 328

Query: 337 IDNAESMPFQQAITTYKVI-QSHHGFTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 396
              +     Q AIT YKV+    +G +W+EL P+T RKHQLRVHCAE LGTPIVGDYKYG
Sbjct: 329 AQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPIVGDYKYG 388

Query: 397 WQAHRNWKPFPS-NFHNNQGKKQSL--PFGLDFENGSIAENNPRLHLHCKQLTLPDVS-- 456
           W  H+ WK  P  +     GK   L  P GLD + GS+    P LHLHC+++ LP+++  
Sbjct: 389 WFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMVLPNIAKF 448

Query: 457 -CALDNDSLDHDCSGL----ECLELDAPLPSYMQRSWDIINN 471
              ++    +   +G+    + L   A +PS+M+ SW+++++
Sbjct: 449 LHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

[Close]
BLAST of Lag0014581 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 75.5 bits (184), Expect = 1.3e-13
Identity = 86/352 (24.43%), Postives = 141/352 (40.06%), Query Frame = 0 

Query: 64  LPPYTATVSGSVLGNRMVNQGARAE--AETSTTAITALKWVLRCCPELPRSLVQKLFRLR 123
           +P   A     ++  R+ N G R E   + +   I    W+      + R+ VQ   RL 
Sbjct: 48  IPNLVACSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLG 107

Query: 124 KVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEEE 183
            V     +++    N++               +GD +   +S +L P         K E 
Sbjct: 108 LVTVNGRVVDKVSHNVK---------------SGDEVNCTIS-ELQPL--------KAEA 167

Query: 184 KFIHSLVLYKDPAILVINKP--------PGLP---VQGGIGVKRSL-------------- 243
           + I   ++Y+D  +LV+NKP        PG P   +  GI    SL              
Sbjct: 168 EDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDS 227

Query: 244 --------DELAASCLSYDYSEPPRLVHRLDRDSSGILIMGRTCTSTSVLHSIFREKTFG 303
                   +E+  S  SY  S  P +VHRLD+ ++G+L++ +   S + L   F+  T  
Sbjct: 228 DEETFSDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHT-- 287

Query: 304 ASKHDVGSEKKILQKRYWALVIGCPRRPKGLIAAPLGKIVLDDGRSEKITVIDNAESMPF 363
                       +++ Y +L  G P  P+G I  P+G+      R     +         
Sbjct: 288 ------------IERVYVSLTTGVPSPPQGRIEIPIGRD--SSNRIRMAAIPGGVRGGRA 347

Query: 364 QQAITTYKVIQSH--HGFTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 379
           + A + YKVI++    G   +E    TGR HQ+R H A+ +G P++GD  YG
Sbjct: 348 RHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVYG 358

[Close]
BLAST of Lag0014581 vs. TAIR 10
Match: AT1G76050.1 (Pseudouridine synthase family protein )

HSP 1 Score: 50.4 bits (119), Expect = 4.4e-06
Identity = 64/278 (23.02%), Postives = 108/278 (38.85%), Query Frame = 0 

Query: 64  LPPYTATVSGSVLGNRMVNQGARAE--AETSTTAITALKWVLRCCPELPRSLVQKLFRLR 123
           +P   A     ++  R+ N G R E   + +   I    W+      + R+ VQ   RL 
Sbjct: 48  IPNLVACSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLG 107

Query: 124 KVRRGSSMIESTECNLEAEEQLRRVAAKDILNTGDRIFLPVSVQLFPKERARDYCNKEEE 183
            V     +++    N++               +GD +   +S +L P         K E 
Sbjct: 108 LVTVNGRVVDKVSHNVK---------------SGDEVNCTIS-ELQPL--------KAEA 167

Query: 184 KFIHSLVLYKDPAILVINKP--------PGLP---VQGGIGVKRSL-------------- 243
           + I   ++Y+D  +LV+NKP        PG P   +  GI    SL              
Sbjct: 168 EDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDS 227

Query: 244 --------DELAASCLSYDYSEPPRLVHRLDRDSSGILIMGRTCTSTSVLHSIFREKTFG 303
                   +E+  S  SY  S  P +VHRLD+ ++G+L++ +   S + L   F+  T  
Sbjct: 228 DEETFSDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHT-- 287

Query: 304 ASKHDVGSEKKILQKRYWALVIGCPRRPKGLIAAPLGK 307
                       +++ Y +L  G P  P+G I  P+G+
Sbjct: 288 ------------IERVYVSLTTGVPSPPQGRIEIPIGR 287

The following BLAST results are available for this feature:
BLAST of Lag0014581 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022148845.12.1e-24487.02RNA pseudouridine synthase 4, mitochondrial [Momordica charantia][more]
XP_023553811.15.3e-23585.81RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
KAG7011636.13.4e-23485.59RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma sub... [more]
XP_022952780.17.6e-23485.59RNA pseudouridine synthase 4, mitochondrial [Cucurbita moschata][more]
KAG6571947.12.9e-23385.38RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma sub... [more]
BLAST of Lag0014581 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LT721.6e-13357.04RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q0J4D41.7e-7938.93RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q5XET63.0e-7636.15RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q69K077.5e-7549.83RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q68XB24.0e-2831.45Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC... [more]
BLAST of Lag0014581 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D4321.0e-24487.02RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1GMP93.7e-23485.59RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IBC55.5e-23084.57RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A5D3C4F52.4e-22582.20RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BZE72.4e-22582.20RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495... [more]
BLAST of Lag0014581 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G19440.11.1e-13457.04Pseudouridine synthase family protein [more]
AT1G78910.12.2e-7736.15Pseudouridine synthase family protein [more]
AT1G76050.21.3e-1324.43Pseudouridine synthase family protein [more]
AT1G76050.14.4e-0623.02Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 195..364
e-value: 8.9E-25
score: 87.6
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 195..429
e-value: 9.99876E-52
score: 171.366
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 176..435
e-value: 3.2E-52
score: 179.2
NoneNo IPR availablePANTHERPTHR21600:SF53RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIALcoord: 40..468
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 40..468
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 183..466

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014581.1Lag0014581.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding