Lag0014553 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014553
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAmino acid permease family protein
Locationchr12: 2054111 .. 2056341 (+)
RNA-Seq ExpressionLag0014553
SyntenyLag0014553
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGAAGAGAGAGGGTCCGATGCTAAAATTGCTCATAAACTAACGATTCTTCCTCTAATCGCTTTGATTTTCTACGACGTTTCTGGAGGACCCTTTGGAGTGGAGGATTCGGTGAGCTGTGGCGGCGGCCCTCTTCTGGCTTTGTTGGGTTTCATAGTGTTTCCGTTTATTTGGAGTATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACAAGTTTCCCCCAGAATGGCGGATATGTGGTATGGATTTCGGCCGCTTTTGGCCCTTTTTGGGGTTTTCAAGAGGGGTTTTGGAAATGGTTCAGTGGGGTAATGGATAATGCTCTGTATCCTGTTCTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAACCATTTGTTAGCTCGAATTCCAGCTCTGTTAGGGATTACAGCCTCTTTAACTTACTTGAACTATCGTGGTCTGCACATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTTTCCATTTGCGGTGATGACCATTCTTTCAATTCCCAGAATAAGGCCCAAAAAGTGGCTAGTTCTGGATTATAGCAAGGTGAATTGGAGGGGTTATTTCAATAGTATGTTTTGGAATCTGAACTATTGGGATAAAGCAAGTACTCTGGCAGGGGAGATTGAAAATCCTAGCAAAACTTTCCCCAAGGCTATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTGATTCCTCTTCTGGCTGGGACTGGTGCCTTGGAGTCGGATTCAAGTGAATGGAGTGATGGGTATTTTGCAGAGGCTGGGGCCTTGATTGGTGGGGTGTGGCTGAAGTGGTGGATTCAAGCTGCTGCTGCCATGTCTAACATGGGGTTGTTTGAAGCTGAAATGAGCAGTGATGCATACCAACTGCTTGGGATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGGTTGGTGGTTTTCAGTTTCCTTTTTCATTTTTCTGTTTTGGTAGCAAGTTTGAGTAACAACAGTCTCTCCCGATGTTCCCACCGATGAGTTTTGTAGCTCAAATCGGTAGCAGTGATTCAAAATAGCAATCCTTGTGAGATGTCAAAAGTTTAAATCACTCTGGAGTAAAATCAAAACTTGTGAGCTCAATTGGCTTGGGTTAACAATATGTTTTAGTGGTAAGTCGACATTGACACGAACCATCACCATAAAAAGGATGATAGGATGAGAAGGATGGATGAGATTGATAGCCTGAAAGTATCATAGAATCCCTAGAACTATAAGACTCCTTGGGGGACTTTTTGCTTCCAAGATGAGTTTGTATAGCTTAAAATTATAAACTAGAGCTTACATTTGTTAGCTGGATTTACTTATGATAAGATACATGTTTGTTAATCCCACGGAACTTCACAAACTCAGGTTACATAAATTCAAACTCTTGAAATACATCTCTCTTGCCACGCCGCCCTAAACACGAGAATCAAAGTAGGTACCTTTCTGCTCTCTAGGTCTATATCCAAGTATGGGGCTATACAAACTCAAACTTTTCAACCTTCAACATGTTGATTAAACAACCATAAGCCAAACATTTCCTAAACTCCTCTCCTATGTCCATATCCTAGTGTGCTTCCTCCTTGAACTCTTCCAGCATTTTGATTCATTTGATTTACTGGCATCTTGTGGATACAAAAGCTGCTCTTATTGAACTACTGTCTATTTCACTTTGCAGATCAAAATATGGGACACCCACCTTCAGCATTCTGTGCTCAGCTTTTGGGGTTATCTTCGTCTCATGGTTGAGTTTCCAAGAAATACTTGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTTTGGAGCTTGCTGCCTTTGTAAAACTAAGAATAAAGAAGCCAGACCTTAACAGACCTTACAGAGTTCCCTTGCAAACATTTGGTGCCACAATGCTTTGCTTGCCACCTACAGCCTTGCTTGTTCTCGTCATGTGTTTAGCTTCTGCACAAACATTCTTAATCAGTGGGATTCTAATTGCTGTGGGGTTTCTTCTATACCCTACTCTTTTGCAGGCAAAGAACAGAAGATGGGCCAAATTCATTTCAGTACAGCCAGCAGCTACTCTTTCTGATGTGGAAGATAGGTTGGCCGAGTCGCAACCAGTGCAAGAAGTTCCTGATGAGGCTGCGGTTCGGCTTCTTTCAGAATCTTATCCGTCTTCGACTATAGCGCAGCAATAA

mRNA sequence

ATGGGCGAAGAGAGAGGGTCCGATGCTAAAATTGCTCATAAACTAACGATTCTTCCTCTAATCGCTTTGATTTTCTACGACGTTTCTGGAGGACCCTTTGGAGTGGAGGATTCGGTGAGCTGTGGCGGCGGCCCTCTTCTGGCTTTGTTGGGTTTCATAGTGTTTCCGTTTATTTGGAGTATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACAAGTTTCCCCCAGAATGGCGGATATGTGGTATGGATTTCGGCCGCTTTTGGCCCTTTTTGGGGTTTTCAAGAGGGGTTTTGGAAATGGTTCAGTGGGGTAATGGATAATGCTCTGTATCCTGTTCTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAACCATTTGTTAGCTCGAATTCCAGCTCTGTTAGGGATTACAGCCTCTTTAACTTACTTGAACTATCGTGGTCTGCACATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTTTCCATTTGCGGTGATGACCATTCTTTCAATTCCCAGAATAAGGCCCAAAAAGTGGCTAGTTCTGGATTATAGCAAGGTGAATTGGAGGGGTTATTTCAATAGTATGTTTTGGAATCTGAACTATTGGGATAAAGCAAGTACTCTGGCAGGGGAGATTGAAAATCCTAGCAAAACTTTCCCCAAGGCTATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTGATTCCTCTTCTGGCTGGGACTGGTGCCTTGGAGTCGGATTCAAGTGAATGGAGTGATGGGTATTTTGCAGAGGCTGGGGCCTTGATTGGTGGGGTGTGGCTGAAGTGGTGGATTCAAGCTGCTGCTGCCATGTCTAACATGGGGTTGTTTGAAGCTGAAATGAGCAGTGATGCATACCAACTGCTTGGGATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGATCAAAATATGGGACACCCACCTTCAGCATTCTGTGCTCAGCTTTTGGGGTTATCTTCGTCTCATGGTTGAGTTTCCAAGAAATACTTGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTTTGGAGCTTGCTGCCTTTGTAAAACTAAGAATAAAGAAGCCAGACCTTAACAGACCTTACAGAGTTCCCTTGCAAACATTTGGTGCCACAATGCTTTGCTTGCCACCTACAGCCTTGCTTGTTCTCGTCATGTGTTTAGCTTCTGCACAAACATTCTTAATCAGTGGGATTCTAATTGCTGTGGGGTTTCTTCTATACCCTACTCTTTTGCAGGCAAAGAACAGAAGATGGGCCAAATTCATTTCAGTACAGCCAGCAGCTACTCTTTCTGATGTGGAAGATAGGTTGGCCGAGTCGCAACCAGTGCAAGAAGTTCCTGATGAGGCTGCGGTTCGGCTTCTTTCAGAATCTTATCCGTCTTCGACTATAGCGCAGCAATAA

Coding sequence (CDS)

ATGGGCGAAGAGAGAGGGTCCGATGCTAAAATTGCTCATAAACTAACGATTCTTCCTCTAATCGCTTTGATTTTCTACGACGTTTCTGGAGGACCCTTTGGAGTGGAGGATTCGGTGAGCTGTGGCGGCGGCCCTCTTCTGGCTTTGTTGGGTTTCATAGTGTTTCCGTTTATTTGGAGTATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACAAGTTTCCCCCAGAATGGCGGATATGTGGTATGGATTTCGGCCGCTTTTGGCCCTTTTTGGGGTTTTCAAGAGGGGTTTTGGAAATGGTTCAGTGGGGTAATGGATAATGCTCTGTATCCTGTTCTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAACCATTTGTTAGCTCGAATTCCAGCTCTGTTAGGGATTACAGCCTCTTTAACTTACTTGAACTATCGTGGTCTGCACATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTTTCCATTTGCGGTGATGACCATTCTTTCAATTCCCAGAATAAGGCCCAAAAAGTGGCTAGTTCTGGATTATAGCAAGGTGAATTGGAGGGGTTATTTCAATAGTATGTTTTGGAATCTGAACTATTGGGATAAAGCAAGTACTCTGGCAGGGGAGATTGAAAATCCTAGCAAAACTTTCCCCAAGGCTATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTGATTCCTCTTCTGGCTGGGACTGGTGCCTTGGAGTCGGATTCAAGTGAATGGAGTGATGGGTATTTTGCAGAGGCTGGGGCCTTGATTGGTGGGGTGTGGCTGAAGTGGTGGATTCAAGCTGCTGCTGCCATGTCTAACATGGGGTTGTTTGAAGCTGAAATGAGCAGTGATGCATACCAACTGCTTGGGATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGATCAAAATATGGGACACCCACCTTCAGCATTCTGTGCTCAGCTTTTGGGGTTATCTTCGTCTCATGGTTGAGTTTCCAAGAAATACTTGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTTTGGAGCTTGCTGCCTTTGTAAAACTAAGAATAAAGAAGCCAGACCTTAACAGACCTTACAGAGTTCCCTTGCAAACATTTGGTGCCACAATGCTTTGCTTGCCACCTACAGCCTTGCTTGTTCTCGTCATGTGTTTAGCTTCTGCACAAACATTCTTAATCAGTGGGATTCTAATTGCTGTGGGGTTTCTTCTATACCCTACTCTTTTGCAGGCAAAGAACAGAAGATGGGCCAAATTCATTTCAGTACAGCCAGCAGCTACTCTTTCTGATGTGGAAGATAGGTTGGCCGAGTCGCAACCAGTGCAAGAAGTTCCTGATGAGGCTGCGGTTCGGCTTCTTTCAGAATCTTATCCGTCTTCGACTATAGCGCAGCAATAA

Protein sequence

MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWSIPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIAVGFLLYPTLLQAKNRRWAKFISVQPAATLSDVEDRLAESQPVQEVPDEAAVRLLSESYPSSTIAQQ
Homology
BLAST of Lag0014553 vs. NCBI nr
Match: XP_038888395.1 (probable polyamine transporter At3g19553 [Benincasa hispida])

HSP 1 Score: 875.5 bits (2261), Expect = 2.1e-250
Identity = 439/486 (90.33%), Postives = 458/486 (94.24%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MG+ER +DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRADAKIAQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIP LLGITASLTYLNYRGLHIVG SAVVLA FSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHVFARIPTLLGITASLTYLNYRGLHIVGVSAVVLAAFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RPKKWLV++YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALESDSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGM+PSVFA+RSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMLPSVFAARSKYGTPTFSILCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LE AAF+KLRIKKPDLNRPYRVPLQTFG TMLC PP ALLVLVMCLASA+TFLISGI++ 
Sbjct: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVT 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPAATLSDVEDRLAESQPVQEVPDEAAVRLLSESYPS 480
           VGFLLYPTLLQAKN+RW KFIS QP ATL DVEDRL ES   QEVP+EA VRLLSES  S
Sbjct: 421 VGFLLYPTLLQAKNKRWVKFISEQPEATLPDVEDRLVESHAQQEVPNEAEVRLLSESSSS 480

Query: 481 STIAQQ 487
           S IAQQ
Sbjct: 481 SNIAQQ 486

BLAST of Lag0014553 vs. NCBI nr
Match: KAA0031359.1 (putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative polyamine transporter [Cucumis melo var. makuwa])

HSP 1 Score: 861.7 bits (2225), Expect = 3.1e-246
Identity = 436/487 (89.53%), Postives = 457/487 (93.84%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MG+ER  DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWL+L+YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LE AAF+KLRI+KPDLNRPY+VPLQT G T+LC PP+ALL+LVMCLASA+TFLISGI+IA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDVEDRLAESQPVQEVPDEAAVRLLSESYP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TLSDVEDRL E QP QEVP+EA VRLLSES  
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES-S 480

Query: 481 SSTIAQQ 487
           SS IAQQ
Sbjct: 481 SSNIAQQ 486

BLAST of Lag0014553 vs. NCBI nr
Match: XP_008455023.1 (PREDICTED: probable polyamine transporter At3g19553 isoform X1 [Cucumis melo])

HSP 1 Score: 860.5 bits (2222), Expect = 6.8e-246
Identity = 435/487 (89.32%), Postives = 457/487 (93.84%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MG+ER  DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWL+L+YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LE AAF+KLRI+KPDLNRPY+VPLQT G T+LC PP+ALL+LVMCLASA+TFLISGI+IA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDVEDRLAESQPVQEVPDEAAVRLLSESYP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TLSDVEDRL E QP QEVP+EA VRLLSES  
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES-S 480

Query: 481 SSTIAQQ 487
           SS IAQQ
Sbjct: 481 SSNIAQQ 486

BLAST of Lag0014553 vs. NCBI nr
Match: XP_004136943.1 (probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypothetical protein Csa_017296 [Cucumis sativus])

HSP 1 Score: 856.3 bits (2211), Expect = 1.3e-244
Identity = 434/487 (89.12%), Postives = 454/487 (93.22%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MG+ER  DAKI  KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWLV++YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALE+DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGM+PSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLY+IGML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LE AAF+KLRI+KPDLNRPY+VPLQTFG T+LC PP ALL LVMCLASA+TFLISGI+IA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDVEDRLAESQPVQEVPDEAAVRLLSESYP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TL DVEDRL ESQ  QEVP+EA VRLLSES  
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSES-S 480

Query: 481 SSTIAQQ 487
           SS IAQQ
Sbjct: 481 SSNIAQQ 486

BLAST of Lag0014553 vs. NCBI nr
Match: XP_023553804.1 (probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.4 bits (2201), Expect = 1.9e-243
Identity = 432/486 (88.89%), Postives = 453/486 (93.21%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MG+ER   A IA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDER---ANIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVF+H+ ARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSL PF VMTILSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RP+KWLV+DYS VNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE+DSSEWSDGYFAE GALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYSIGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LELAAF+KLR+KKPDL+RPY+VPLQTFGA MLCLPP ALLVLVMCLASAQTFLISG++IA
Sbjct: 361 LELAAFIKLRMKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPAATLSDVEDRLAESQPVQEVPDEAAVRLLSESYPS 480
           VGFLLYP+L QAKNR W KFIS +  ATL DVEDRL E QP +EV DEA +RLLSES  S
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISDEQGATLPDVEDRLVEPQPREEVLDEAVIRLLSESPSS 480

Query: 481 STIAQQ 487
             IA Q
Sbjct: 481 PNIANQ 483

BLAST of Lag0014553 vs. ExPASy Swiss-Prot
Match: Q9LH39 (Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g19553 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.0e-204
Identity = 354/477 (74.21%), Postives = 410/477 (85.95%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           + +E  S  K + KLT+LPL+ LIFY+VSGGPFGVEDSV  GGGPLLALLGF++FP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFP+NGGYVVWIS+AFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
            SFPV +H+ AR+PALL IT SLTYLNYRGLHIVGFSAVVLAVFSL PF VM +L++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RPK+WL +D  K+NWRGYFN+MFWNLNYWDKASTLAGE++ P KTFPKA+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALESDSS-EWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL S +S EWSDGYFAE G LIGGVWLK WIQAAAAMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGM 360
           A+QLLGMSE+GM+P+ FA RSKYGTPT SILCSA GVIF+SW+SFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILI 420
           LLE AAFVKLRIKKPDL+RPYRVPL TFG +MLCLPP+ L++LVM LA+ +TFLISG++I
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 AVGFLLYPTLLQAKNRRWAKFI--SVQPAATLSDVEDRLAESQPVQEVPDEAAVRLL 475
            +GF LYP L   K ++WA+FI    +P + +S      +ESQ  +E  DE+A  LL
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFIPEETRPVSGVS------SESQLDEEHGDESAASLL 478

BLAST of Lag0014553 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.7e-151
Identity = 254/451 (56.32%), Postives = 341/451 (75.61%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF++FPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SGV+DNALYPVLFLDYLK   P     L R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKV 193
            ++L +T  LTYLNYRGL IVG+ AV++ VFS+ PFAVM ++SIP++ P +WLV+D   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  STLAGE+ENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 254 SDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            +  +W+DGYF++    +GG WL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
             FA RS+YGTP   IL SA GV+ +SWLSFQEI+   N LY +GM+LE  AFV++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 374 PDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIAVGFLLYPTLLQAK 433
           P  +RPY++P+ T G+ ++C+PPT L+  V+ L+S +   +S +++ +GFL++P L    
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 434 NRRWAKFISVQPAATLSDVEDRLAESQPVQE 465
            +RW KF      +  SD+ D   +++  +E
Sbjct: 468 RKRWVKF------SISSDLPDLQQQTREYEE 491

BLAST of Lag0014553 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.4e-148
Identity = 251/429 (58.51%), Postives = 327/429 (76.22%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWSIPEALVTAELATS 73
           K+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GFIVFPFIWSIPEAL+TAE+ T 
Sbjct: 52  KITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 111

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           FP+NGGYVVW++ A GP+WGFQ+G+ KW SGV+DNALYP+LFLDYLK   P+    + R+
Sbjct: 112 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 171

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLD--YS 193
            A+L +T +LTYLNYRGL IVG +AV+L VFS+ PF VM+ +SIP+++P +WLV+     
Sbjct: 172 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 231

Query: 194 KVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 253
            VNW  Y N++FWNLNYWD  STL GE+ENPSKT P+A+F A++LVV SY+ P+L GTGA
Sbjct: 232 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 291

Query: 254 LESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGM 313
           +  D   W+DGYFA+ G +IGGVWL WWIQAAAA SNMG+F AEMSSD++QLLGM+E GM
Sbjct: 292 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 351

Query: 314 IPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRI 373
           +P VFA RS+Y TP   IL SA GVI +SWLSFQEI+   N LY  GM+LE   FV+LR+
Sbjct: 352 LPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRM 411

Query: 374 KKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIAVGFLLYPTLLQ 433
           K P  +RP+++P+   G+ ++C+PPT L+ ++M   + +  L+S   I +G +L P L Q
Sbjct: 412 KYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQ 471

Query: 434 AKNRRWAKF 441
            + + W KF
Sbjct: 472 VEKKGWLKF 479

BLAST of Lag0014553 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 7.6e-147
Identity = 253/444 (56.98%), Postives = 332/444 (74.77%), Query Frame = 0

Query: 2   GEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWSI 61
           G+  G     A  ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GF+V P IWSI
Sbjct: 70  GDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSI 129

Query: 62  PEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKR 121
           PEAL+TAEL   FP+NGGYVVW+++A GP+WGFQ+G+ KW SGV+DNALYPVLFLDYLK 
Sbjct: 130 PEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKS 189

Query: 122 SFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIR 181
             P       R  A++G+TA LT LNYRGL +VG+ A+ L VFSL PF VM ++++P++R
Sbjct: 190 GVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLR 249

Query: 182 PKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSY 241
           P +WLV+D   V+W  Y N++FWNLNYWD  STLAGE++NP KT PKA+F AV+ VV +Y
Sbjct: 250 PARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAY 309

Query: 242 LIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAY 301
           L PLLAGTGA+  D  +W+DGYFA+   L+GG WL WW+Q+AAA+SNMG+F AEMSSD+Y
Sbjct: 310 LYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSY 369

Query: 302 QLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLL 361
           QLLGM+E GM+PS FA+RS+YGTP   IL SA GV+ +S +SFQEI+   NFLY  GMLL
Sbjct: 370 QLLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLL 429

Query: 362 ELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIAV 421
           E  AF+  R+++PD  RPYRVPL T G   + +PPTAL+ +V+ L++ +  ++S   +A+
Sbjct: 430 EFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAM 489

Query: 422 GFLLYPTLLQAKNRRWAKFISVQP 446
           G +L P L   + +RW +F SV P
Sbjct: 490 GLVLQPALRFVEKKRWLRF-SVNP 511

BLAST of Lag0014553 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 7.6e-147
Identity = 253/444 (56.98%), Postives = 332/444 (74.77%), Query Frame = 0

Query: 2   GEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWSI 61
           G+  G     A  ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GF+V P IWSI
Sbjct: 70  GDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSI 129

Query: 62  PEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKR 121
           PEAL+TAEL   FP+NGGYVVW+++A GP+WGFQ+G+ KW SGV+DNALYPVLFLDYLK 
Sbjct: 130 PEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKS 189

Query: 122 SFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIR 181
             P       R  A++G+TA LT LNYRGL +VG+ A+ L VFSL PF VM ++++P++R
Sbjct: 190 GVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLR 249

Query: 182 PKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSY 241
           P +WLV+D   V+W  Y N++FWNLNYWD  STLAGE++NP KT PKA+F AV+ VV +Y
Sbjct: 250 PARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAY 309

Query: 242 LIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAY 301
           L PLLAGTGA+  D  +W+DGYFA+   L+GG WL WW+Q+AAA+SNMG+F AEMSSD+Y
Sbjct: 310 LYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSY 369

Query: 302 QLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLL 361
           QLLGM+E GM+PS FA+RS+YGTP   IL SA GV+ +S +SFQEI+   NFLY  GMLL
Sbjct: 370 QLLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLL 429

Query: 362 ELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIAV 421
           E  AF+  R+++PD  RPYRVPL T G   + +PPTAL+ +V+ L++ +  ++S   +A+
Sbjct: 430 EFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAM 489

Query: 422 GFLLYPTLLQAKNRRWAKFISVQP 446
           G +L P L   + +RW +F SV P
Sbjct: 490 GLVLQPALRFVEKKRWLRF-SVNP 511

BLAST of Lag0014553 vs. ExPASy TrEMBL
Match: A0A5D3C4H2 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001140 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.5e-246
Identity = 436/487 (89.53%), Postives = 457/487 (93.84%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MG+ER  DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWL+L+YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LE AAF+KLRI+KPDLNRPY+VPLQT G T+LC PP+ALL+LVMCLASA+TFLISGI+IA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDVEDRLAESQPVQEVPDEAAVRLLSESYP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TLSDVEDRL E QP QEVP+EA VRLLSES  
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES-S 480

Query: 481 SSTIAQQ 487
           SS IAQQ
Sbjct: 481 SSNIAQQ 486

BLAST of Lag0014553 vs. ExPASy TrEMBL
Match: A0A1S3C014 (probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495296 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.3e-246
Identity = 435/487 (89.32%), Postives = 457/487 (93.84%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MG+ER  DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWL+L+YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LE AAF+KLRI+KPDLNRPY+VPLQT G T+LC PP+ALL+LVMCLASA+TFLISGI+IA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDVEDRLAESQPVQEVPDEAAVRLLSESYP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TLSDVEDRL E QP QEVP+EA VRLLSES  
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES-S 480

Query: 481 SSTIAQQ 487
           SS IAQQ
Sbjct: 481 SSNIAQQ 486

BLAST of Lag0014553 vs. ExPASy TrEMBL
Match: A0A0A0K2Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 6.3e-245
Identity = 434/487 (89.12%), Postives = 454/487 (93.22%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MG+ER  DAKI  KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWLV++YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALE+DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGM+PSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLY+IGML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LE AAF+KLRI+KPDLNRPY+VPLQTFG T+LC PP ALL LVMCLASA+TFLISGI+IA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDVEDRLAESQPVQEVPDEAAVRLLSESYP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TL DVEDRL ESQ  QEVP+EA VRLLSES  
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSES-S 480

Query: 481 SSTIAQQ 487
           SS IAQQ
Sbjct: 481 SSNIAQQ 486

BLAST of Lag0014553 vs. ExPASy TrEMBL
Match: A0A6J1I9Z5 (probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC111471008 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.6e-243
Identity = 433/486 (89.09%), Postives = 452/486 (93.00%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MG+ER   AKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDER---AKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVF+H+ ARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSL PF VMTILSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RP+KWLV+DYS VNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE+DSS+WSDGYFAE GALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSDWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYSIGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LELAAF+KLRIKKPDL+RPY+VPLQTFGA MLCLPP ALLVLVMCLASAQTFLISGI+I+
Sbjct: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGIIIS 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPAATLSDVEDRLAESQPVQEVPDEAAVRLLSESYPS 480
           VGFLLYP+L QAKNR W KFIS Q   TL DVED L E QP QEV DEA +RLLSES  S
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDEAVIRLLSESPSS 480

Query: 481 STIAQQ 487
             IA Q
Sbjct: 481 PNIANQ 483

BLAST of Lag0014553 vs. ExPASy TrEMBL
Match: A0A6J1D4Y1 (probable polyamine transporter At3g19553 OS=Momordica charantia OX=3673 GN=LOC111017345 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.3e-242
Identity = 428/477 (89.73%), Postives = 451/477 (94.55%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           MGEERG+DAKIA KLTILPL+ALIFYDVSGGPFGVEDSVS GGGPLLA+LGF++FPFIWS
Sbjct: 1   MGEERGADAKIAPKLTILPLVALIFYDVSGGPFGVEDSVSTGGGPLLAILGFLIFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFG FWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGHFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPV NHL ARIPAL GIT SLTYLNYRGLHIVGFSAVVLAVFSL PF VM ILSIPRI
Sbjct: 121 RSFPVLNHLFARIPALFGITVSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMAILSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RPK+WLV+++SKVNWRGYFNSMFWNLNYWDKAST+AGE+ENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 RPKRWLVVEFSKVNWRGYFNSMFWNLNYWDKASTVAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALES+SSEWSDGYFAE GALIGGVWLKWWI+AAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALESNSSEWSDGYFAEVGALIGGVWLKWWIEAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           +QLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIF+SW+SFQEILEFLNFLYSIGML
Sbjct: 301 FQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIA 420
           LELAAFVKLRIKKPDL+RPYRVPLQTFGATMLCLPP  LLVLVM LAS +TFL+SG +IA
Sbjct: 361 LELAAFVKLRIKKPDLHRPYRVPLQTFGATMLCLPPALLLVLVMSLASLRTFLVSGFIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPAATLSDVEDRLAESQPVQEVPDEAAVRLLSES 478
           VGFLLYPTLL AK+RRWAKFIS  PAA L DVED+ AESQP   +PDEAAVRLLSES
Sbjct: 421 VGFLLYPTLLLAKSRRWAKFISQHPAAPLPDVEDQPAESQP---LPDEAAVRLLSES 474

BLAST of Lag0014553 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 712.6 bits (1838), Expect = 2.1e-205
Identity = 354/477 (74.21%), Postives = 410/477 (85.95%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWS 60
           + +E  S  K + KLT+LPL+ LIFY+VSGGPFGVEDSV  GGGPLLALLGF++FP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFP+NGGYVVWIS+AFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
            SFPV +H+ AR+PALL IT SLTYLNYRGLHIVGFSAVVLAVFSL PF VM +L++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RPK+WL +D  K+NWRGYFN+MFWNLNYWDKASTLAGE++ P KTFPKA+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALESDSS-EWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL S +S EWSDGYFAE G LIGGVWLK WIQAAAAMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGM 360
           A+QLLGMSE+GM+P+ FA RSKYGTPT SILCSA GVIF+SW+SFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILI 420
           LLE AAFVKLRIKKPDL+RPYRVPL TFG +MLCLPP+ L++LVM LA+ +TFLISG++I
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 AVGFLLYPTLLQAKNRRWAKFI--SVQPAATLSDVEDRLAESQPVQEVPDEAAVRLL 475
            +GF LYP L   K ++WA+FI    +P + +S      +ESQ  +E  DE+A  LL
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFIPEETRPVSGVS------SESQLDEEHGDESAASLL 478

BLAST of Lag0014553 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 537.3 bits (1383), Expect = 1.2e-152
Identity = 254/451 (56.32%), Postives = 341/451 (75.61%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF++FPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SGV+DNALYPVLFLDYLK   P     L R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKV 193
            ++L +T  LTYLNYRGL IVG+ AV++ VFS+ PFAVM ++SIP++ P +WLV+D   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  STLAGE+ENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 254 SDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            +  +W+DGYF++    +GG WL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
             FA RS+YGTP   IL SA GV+ +SWLSFQEI+   N LY +GM+LE  AFV++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 374 PDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIAVGFLLYPTLLQAK 433
           P  +RPY++P+ T G+ ++C+PPT L+  V+ L+S +   +S +++ +GFL++P L    
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 434 NRRWAKFISVQPAATLSDVEDRLAESQPVQE 465
            +RW KF      +  SD+ D   +++  +E
Sbjct: 468 RKRWVKF------SISSDLPDLQQQTREYEE 491

BLAST of Lag0014553 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 537.3 bits (1383), Expect = 1.2e-152
Identity = 254/451 (56.32%), Postives = 341/451 (75.61%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF++FPFIWSIPEAL+TAE+ T 
Sbjct: 32  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 91

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SGV+DNALYPVLFLDYLK   P     L R+
Sbjct: 92  YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 151

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKV 193
            ++L +T  LTYLNYRGL IVG+ AV++ VFS+ PFAVM ++SIP++ P +WLV+D   V
Sbjct: 152 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 211

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  STLAGE+ENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 212 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 271

Query: 254 SDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            +  +W+DGYF++    +GG WL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 272 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 331

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
             FA RS+YGTP   IL SA GV+ +SWLSFQEI+   N LY +GM+LE  AFV++R+K 
Sbjct: 332 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 391

Query: 374 PDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIAVGFLLYPTLLQAK 433
           P  +RPY++P+ T G+ ++C+PPT L+  V+ L+S +   +S +++ +GFL++P L    
Sbjct: 392 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 451

Query: 434 NRRWAKFISVQPAATLSDVEDRLAESQPVQE 465
            +RW KF      +  SD+ D   +++  +E
Sbjct: 452 RKRWVKF------SISSDLPDLQQQTREYEE 475

BLAST of Lag0014553 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 527.7 bits (1358), Expect = 9.8e-150
Identity = 251/429 (58.51%), Postives = 327/429 (76.22%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWSIPEALVTAELATS 73
           K+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GFIVFPFIWSIPEAL+TAE+ T 
Sbjct: 52  KITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 111

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           FP+NGGYVVW++ A GP+WGFQ+G+ KW SGV+DNALYP+LFLDYLK   P+    + R+
Sbjct: 112 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 171

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLD--YS 193
            A+L +T +LTYLNYRGL IVG +AV+L VFS+ PF VM+ +SIP+++P +WLV+     
Sbjct: 172 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 231

Query: 194 KVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 253
            VNW  Y N++FWNLNYWD  STL GE+ENPSKT P+A+F A++LVV SY+ P+L GTGA
Sbjct: 232 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 291

Query: 254 LESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGM 313
           +  D   W+DGYFA+ G +IGGVWL WWIQAAAA SNMG+F AEMSSD++QLLGM+E GM
Sbjct: 292 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 351

Query: 314 IPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRI 373
           +P VFA RS+Y TP   IL SA GVI +SWLSFQEI+   N LY  GM+LE   FV+LR+
Sbjct: 352 LPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRM 411

Query: 374 KKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIAVGFLLYPTLLQ 433
           K P  +RP+++P+   G+ ++C+PPT L+ ++M   + +  L+S   I +G +L P L Q
Sbjct: 412 KYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQ 471

Query: 434 AKNRRWAKF 441
            + + W KF
Sbjct: 472 VEKKGWLKF 479

BLAST of Lag0014553 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 499.6 bits (1285), Expect = 2.9e-141
Identity = 241/427 (56.44%), Postives = 319/427 (74.71%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFIVFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFG E SV+   GPLLALLGF++FPFIW IPEAL+TAE++T 
Sbjct: 35  KVSMLPLVFLIFYEVSGGPFGAEGSVN-AAGPLLALLGFVIFPFIWCIPEALITAEMSTM 94

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           FP NGG+VVW+S+A G FWGFQ G+ KW  GV+DNALYPVLFLDYLK + P     L R+
Sbjct: 95  FPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNALYPVLFLDYLKSAVPALATGLPRV 154

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKV 193
            ++L +T  LTYLNYRGL IVG++AV + VFS+ PFAVM+++SIP++ P +WLV+D   V
Sbjct: 155 ASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPFAVMSLVSIPQLEPSRWLVMDLGNV 214

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++ WNLNYWD  STLAGE+ NP KT PKA+   V+ V  S  +PLL+GTGA+ 
Sbjct: 215 NWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKALCYGVIFVALSNFLPLLSGTGAIP 274

Query: 254 SDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            D   W+DGY AE    IGG WL+ W+QAAAA SNMG+F AEMSSD++QLLGM+E+G++P
Sbjct: 275 LDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNMGMFLAEMSSDSFQLLGMAELGILP 334

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
            +FA RS+YGTP   IL SA GV+ +S LSFQEI+   N LY  GM+LE  AFV+LR K 
Sbjct: 335 EIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIAAENLLYCGGMILEFIAFVRLRKKH 394

Query: 374 PDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGILIAVGFLLYPTLLQAK 433
           P  +RPY++P+ T G+ ++C+PP  L+ LV+ L++ +  L+S +++ +GFL+ P L    
Sbjct: 395 PAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTIKVALVSFVMVVIGFLMKPCLNHMD 454

Query: 434 NRRWAKF 441
            ++W KF
Sbjct: 455 GKKWVKF 460

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888395.12.1e-25090.33probable polyamine transporter At3g19553 [Benincasa hispida][more]
KAA0031359.13.1e-24689.53putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative p... [more]
XP_008455023.16.8e-24689.32PREDICTED: probable polyamine transporter At3g19553 isoform X1 [Cucumis melo][more]
XP_004136943.11.3e-24489.12probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypotheti... [more]
XP_023553804.11.9e-24388.89probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LH393.0e-20474.21Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9C6S51.7e-15156.32Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Q9FFL11.4e-14858.51Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
A2X8M87.6e-14756.98Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D07.6e-14756.98Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Match NameE-valueIdentityDescription
A0A5D3C4H21.5e-24689.53Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3C0143.3e-24689.32probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
A0A0A0K2Y26.3e-24589.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1[more]
A0A6J1I9Z52.6e-24389.09probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A6J1D4Y11.3e-24289.73probable polyamine transporter At3g19553 OS=Momordica charantia OX=3673 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G19553.12.1e-20574.21Amino acid permease family protein [more]
AT1G31830.11.2e-15256.32Amino acid permease family protein [more]
AT1G31830.21.2e-15256.32Amino acid permease family protein [more]
AT5G05630.19.8e-15058.51Amino acid permease family protein [more]
AT1G31820.12.9e-14156.44Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 20..415
e-value: 5.1E-32
score: 111.3
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 1..450
e-value: 1.6E-65
score: 218.9
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 13..446
e-value: 3.9E-56
score: 192.5
NoneNo IPR availablePANTHERPTHR45826:SF21BNAA05G20530D PROTEINcoord: 5..474
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 5..474

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014553.1Lag0014553.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity