Lag0014543 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014543
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H-like superfamily protein
Locationchr12: 1930806 .. 1932043 (-)
RNA-Seq ExpressionLag0014543
SyntenyLag0014543
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCTGGTATGGTTATTGTCATGTTTGCCAAGAGGAGACTGAGACTACCAAGCATGCCCTTTTCCAATGTTCGAAAGCCCGAGCGATTTGGGAGTTATTACTTCCGTTAATGTACGACGATCAATGGGGTATAGTGGATATCAAAGATCTTTGGTTGGGATTGACTGATTGCCAAATGTCGGATCTTGAACGTATCTGTGTGGGGGCTTGGGCCATTTGGAATGATAGAAATAGTTGGATTCACAATCATCCAATTCATGACACAGTGTCTAGGGCGGATTGGATAAATATGTATCTGACAGAATTTTGGCAGGCAAACCCTAAGGGTGGTATTCCAATCCAGTCCGAGAAAGATATTTTGGAGATCATCTCCCGAGGCGAGGAGACTATTTTGCACACTGATGCAGCGTTTATGGGAGACCGAGACACAGGTGGTATTGGGTTAGTAATGCGGGACAAATTGGGGCAACTGCTTGCAATAAAATCATCAGGTTTTTCGAACTTTTCATCCCCTTTGGTGGGGGAAGTGATTGTGGTGCTAGAAGGACTTCGAATGGCACGAGCTTTTGATGTGAGGAGGTTGATGATTTTGTCTGACTTGTTGAACTTAATTAACATCATTAACGAGGAGATACAAGGTCATTCTAGTATTGCAACAGTTATCTGGGATATTAAAGTCATGAGGAATTTTTTTGAGAATGTAAGTTTTAAATTTGTTAGTCGAAAGTTTAATAGGTTTGCTCATCAAACGGCCCGTGTTGGGTTATCTTCTCCTTCACCGATTTTGTGGCAAGGAAACTATCCTGATTGGATGGTTGGGTTATCGCTCACAGAATGTAGTTTGTTTGTACCCCTGAGGTTCTATTATTATCTAATGAGAATTTTCCTATTTTTCAAAAAAAAAAAAAAAAAAAAAGTTAAACCAAACACACCCTTAGATCTATGGATTTAATTTTGTATCTAATGAGTCTTTTCAATCTTATTTAAAAATTCAAAAATTTGTAAGTTTCGGACTTATCAAACAATAAAAGTGAACATACTTTGGTGGTTTTGATTAAGATGTCAAATGTTCGAATTCTTTCAGTTTTTTTTTTTTAAGAAAATTAACTATTTAAACACGGAAAAATTGAAAATTTATCCAAAGAATCTATTTTTCTAGTAGGAAACTAACTATTTAATGGAAGATTTTTCATGAATAATTAACTATCATTGAAACTTTATAGATATTGCTCATGA

mRNA sequence

ATGTGCTGGTATGGTTATTGTCATGTTTGCCAAGAGGAGACTGAGACTACCAAGCATGCCCTTTTCCAATGTTCGAAAGCCCGAGCGATTTGGGAGTTATTACTTCCGTTAATGTACGACGATCAATGGGGTATAGTGGATATCAAAGATCTTTGGTTGGGATTGACTGATTGCCAAATGTCGGATCTTGAACGTATCTGTGTGGGGGCTTGGGCCATTTGGAATGATAGAAATAGTTGGATTCACAATCATCCAATTCATGACACAGTGTCTAGGGCGGATTGGATAAATATGTATCTGACAGAATTTTGGCAGGCAAACCCTAAGGGTGGTATTCCAATCCAGTCCGAGAAAGATATTTTGGAGATCATCTCCCGAGGCGAGGAGACTATTTTGCACACTGATGCAGCGTTTATGGGAGACCGAGACACAGGTGGTATTGGGTTAGTAATGCGGGACAAATTGGGGCAACTGCTTGCAATAAAATCATCAGGTTTTTCGAACTTTTCATCCCCTTTGGTGGGGGAAGTGATTGTGGTGCTAGAAGGACTTCGAATGGCACGAGCTTTTGATGTGAGGAGGTTGATGATTTTGTCTGACTTGTTGAACTTAATTAACATCATTAACGAGGAGATACAAGGTCATTCTAGTATTGCAACAGTTATCTGGGATATTAAAGTCATGAGGAATTTTTTTGAGAATGTAAGTTTTAAATTTGTTAGTCGAAAGTTTAATAGGTTTGCTCATCAAACGGCCCGTGTTGGGTTATCTTCTCCTTCACCGATTTTGTGGCAAGGAAACTATCCTGATTGGATGGTTGGGTTATCGCTCACAGAATGTAGTTTGTTTGTACCCCTGAGATATTGCTCATGA

Coding sequence (CDS)

ATGTGCTGGTATGGTTATTGTCATGTTTGCCAAGAGGAGACTGAGACTACCAAGCATGCCCTTTTCCAATGTTCGAAAGCCCGAGCGATTTGGGAGTTATTACTTCCGTTAATGTACGACGATCAATGGGGTATAGTGGATATCAAAGATCTTTGGTTGGGATTGACTGATTGCCAAATGTCGGATCTTGAACGTATCTGTGTGGGGGCTTGGGCCATTTGGAATGATAGAAATAGTTGGATTCACAATCATCCAATTCATGACACAGTGTCTAGGGCGGATTGGATAAATATGTATCTGACAGAATTTTGGCAGGCAAACCCTAAGGGTGGTATTCCAATCCAGTCCGAGAAAGATATTTTGGAGATCATCTCCCGAGGCGAGGAGACTATTTTGCACACTGATGCAGCGTTTATGGGAGACCGAGACACAGGTGGTATTGGGTTAGTAATGCGGGACAAATTGGGGCAACTGCTTGCAATAAAATCATCAGGTTTTTCGAACTTTTCATCCCCTTTGGTGGGGGAAGTGATTGTGGTGCTAGAAGGACTTCGAATGGCACGAGCTTTTGATGTGAGGAGGTTGATGATTTTGTCTGACTTGTTGAACTTAATTAACATCATTAACGAGGAGATACAAGGTCATTCTAGTATTGCAACAGTTATCTGGGATATTAAAGTCATGAGGAATTTTTTTGAGAATGTAAGTTTTAAATTTGTTAGTCGAAAGTTTAATAGGTTTGCTCATCAAACGGCCCGTGTTGGGTTATCTTCTCCTTCACCGATTTTGTGGCAAGGAAACTATCCTGATTGGATGGTTGGGTTATCGCTCACAGAATGTAGTTTGTTTGTACCCCTGAGATATTGCTCATGA

Protein sequence

MCWYGYCHVCQEETETTKHALFQCSKARAIWELLLPLMYDDQWGIVDIKDLWLGLTDCQMSDLERICVGAWAIWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGIPIQSEKDILEIISRGEETILHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPLVGEVIVVLEGLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSFKFVSRKFNRFAHQTARVGLSSPSPILWQGNYPDWMVGLSLTECSLFVPLRYCS
Homology
BLAST of Lag0014543 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 134.4 bits (337), Expect = 1.5e-27
Identity = 89/279 (31.90%), Postives = 131/279 (46.95%), Query Frame = 0

Query: 7    CHVCQEETETTKHALFQCSKARAIWELLLP----LMYDDQWGIVDIKDLWLGLTD-CQMS 66
            C +C +  E+  HA F C +AR IW  L P    L  +D    +   +LW  LT+  +  
Sbjct: 1173 CTICGDRRESIIHAFFHCKRARQIWRTLFPFLTCLSAEDN---ISFLELWSSLTEQLEPK 1232

Query: 67   DLERICVGAWAIWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGIP-IQSEKDI 126
            DL    +  W IWNDRNS IH   +     + +W+  +L    QA      P  QS    
Sbjct: 1233 DLNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHRP 1292

Query: 127  LEIISRGEETI---LHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPLVGEV 186
            +    R   ++   L+TDAA  G   +   G ++RD    L+A  S       SPL+ E+
Sbjct: 1293 VVQYWRPSSSVSLKLNTDAACRG--ASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEI 1352

Query: 187  IVVLEGLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSF 246
              +LEGL+ A A +   L + SD L  I +I  EI         + +I+ +   F  +SF
Sbjct: 1353 RCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISF 1412

Query: 247  KFVSRKFNRFAHQTARVGLSSPS-PILWQGNYPDWMVGL 276
               SR+ NR AH  A+ G++SPS    W  N+P W++ L
Sbjct: 1413 SHSSRQCNRAAHGLAKWGITSPSATYAWLFNFPTWLLDL 1446

BLAST of Lag0014543 vs. NCBI nr
Match: OMO70721.1 (Zinc finger, CCHC-type [Corchorus olitorius])

HSP 1 Score: 113.6 bits (283), Expect = 2.8e-21
Identity = 79/277 (28.52%), Postives = 122/277 (44.04%), Query Frame = 0

Query: 7   CHVCQEETETTKHALFQCSKARAIWELLLPLMYDDQWGIVDIKDLWLGLTDCQMSDLERI 66
           C  C +E ET  HA+ +C KA  +WEL+       +   VD     LG  D    D+   
Sbjct: 696 CIRCGKEEETMAHAIKECEKAEQVWELISEHYVASKNDEVDGSQTKLG--DLNWGDI--A 755

Query: 67  CVGAWAIWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGIPIQSEKDILEIISR 126
            V  WAIW  RN  IH++ ++     A ++  YL E+ +   K   P  +++   E   +
Sbjct: 756 LVTTWAIWGARNKEIHDNIVYSATETAGFVISYLMEYQRCKRKMQ-PNLAKETHWEAPGQ 815

Query: 127 GEETILHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPLVGEVIVVLEGLRM 186
            E  + + D A+     TG  G + RD  G +L   +      S   V E    L+  + 
Sbjct: 816 SEIKV-NFDGAYDSRGKTGAFGAIARDSAGLVLGAYAGRLGFVSDVYVAECKAALKAAKW 875

Query: 187 ARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSFKFVSRKFNR 246
           AR     R+++  D L +I   N      S I   I D+K  +N+FE  SF  V RK NR
Sbjct: 876 ARNMGFTRIILEGDALTVIKKANSSETDLSPITAYIADLKEAQNWFEKCSFSHVGRKGNR 935

Query: 247 FAHQTARVGLSSPSPILWQGNYPDWMVGLSLTECSLF 284
                A++G S    I+W  + P ++  +   +C+ F
Sbjct: 936 ATDVLAQLGKSYSESIIWMEDVPVYLSEIIQQDCTGF 966

BLAST of Lag0014543 vs. NCBI nr
Match: XP_030508852.1 (uncharacterized protein LOC115723496 [Cannabis sativa])

HSP 1 Score: 110.2 bits (274), Expect = 3.1e-20
Identity = 81/287 (28.22%), Postives = 133/287 (46.34%), Query Frame = 0

Query: 6    YCHVCQEETETTKHALFQCSKARAIWEL---LLPLMYDDQWGIVDIKDLWLGLTDCQMSD 65
            YC +C    ET  HALF C +A+A+WEL    +     ++    D   L L  T    S+
Sbjct: 1092 YCTICNSCEETVHHALFSCPRAKAVWELSNFSIDFQTIERSSTAD--TLLLLSTSLSSSE 1151

Query: 66   LERICVGAWAIWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGIPIQS------ 125
            LE   V  W+IW++RN+  H + +    + A +   YLTEF QA  K   P+ +      
Sbjct: 1152 LELFLVLCWSIWHERNAIYHGNSVRTPAAVAAYAPSYLTEFQQARAKNAKPVTASGAATP 1211

Query: 126  EKDILEIISRGEETI-------LHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGF-SN 185
             +   E I   + T        L+TDAA   +R+T GIG V+R+  G ++A  S  F  N
Sbjct: 1212 SRPSSEFIHAPKWTTPPRGRLKLNTDAAIDKERNTIGIGAVLRNSDGIIVAALSKPFRGN 1271

Query: 186  FSSPLVGEVIVVLEGLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVM 245
            F +  + E + +   L    + ++    I +D L ++  +       S+   ++ +I  +
Sbjct: 1272 FKAEEM-EALGLALSLNWLLSHNLSVDFIETDSLLVVQGLKTSHSFLSAFHALLNNINYL 1331

Query: 246  RNFFENVSFKFVSRKFNRFAHQTARVGLSSPSPILWQGNYPDWMVGL 276
             +FF       VSR  N +AH  A+  L+  +  +W G +P  ++ L
Sbjct: 1332 VSFFPRAQIDHVSRSANTYAHTLAKFALTVDTDCIWLGTFPSPLMTL 1375

BLAST of Lag0014543 vs. NCBI nr
Match: XP_038704726.1 (uncharacterized protein LOC120000672 [Tripterygium wilfordii])

HSP 1 Score: 108.6 bits (270), Expect = 9.1e-20
Identity = 71/264 (26.89%), Postives = 124/264 (46.97%), Query Frame = 0

Query: 15  ETTKHALFQCSKARAIWELLLPLMYDDQWGIVDIKDLWLG--LTDCQMSDLERICVGAWA 74
           E   HAL +C  A  +W+    + Y  +   +  +D WL   L   +    +R+   AWA
Sbjct: 2   ENVSHALLECKWASVVWQEAGCVAYQSKERDLSFED-WLSQVLLGGEGEVAKRVMYYAWA 61

Query: 75  IWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGI--PIQSEKDILEIISRGEET 134
           +W ++NS+I         S  +++  Y+ E+ QA     +  P QS ++ ++       T
Sbjct: 62  VWTNKNSFIFEGKWRSATSTINFVEYYMAEYDQARASRNLPRPEQSVQEAVKWAPPSSGT 121

Query: 135 I-LHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPLVGEVIVVLEGLRMARA 194
           I L  D A   +    G+G V+RD  GQ+ A  S   +    P V +   +++G ++A  
Sbjct: 122 IKLSVDRAVFNEAHAIGVGAVIRDHNGQVCAALSECMAGAFGPQVVKAYALMKGTQLAIH 181

Query: 195 FDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSFKFVSRKFNRFAH 254
             V  L+I SD   L+N I  E    +    ++ DI+ + +   +VS ++  R  NR AH
Sbjct: 182 MGVSNLIIESDSHVLVNAIRSEAYELAPFGHILDDIRSLISCIGSVSLRYTRRLCNRVAH 241

Query: 255 QTARVGLSSPSPILWQGNYPDWMV 274
           + AR  ++   P+ W G  PD+++
Sbjct: 242 KLARNSINFDCPMRWIGECPDFIL 264

BLAST of Lag0014543 vs. NCBI nr
Match: TXG50387.1 (hypothetical protein EZV62_022911 [Acer yangbiense])

HSP 1 Score: 108.2 bits (269), Expect = 1.2e-19
Identity = 72/267 (26.97%), Postives = 124/267 (46.44%), Query Frame = 0

Query: 6   YCHVCQEETETTKHALFQCSKARAIWELLLPLMYDDQWGIVDIKDLWLGL---TDCQMSD 65
           YC VC +++E+  H L+ C+ A  +W  LL      +  + D   + L L    D  + +
Sbjct: 157 YCQVCNDDSESITHILWSCNSAVEVWRQLLGDDVIQRIVVSDFPSIILSLWRSVDSVVFN 216

Query: 66  LERICVGAWAIWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGIPIQSEKDILE 125
           L  + +G W +W +RNS +H            WI+ +  EF  AN      + S +   +
Sbjct: 217 L--LIIGYWRLWTNRNSVVHGSAGWAAADMVTWIDNFANEFRLANELNHKEVLSHQPSWK 276

Query: 126 IISRGEETILHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPLVGEVIVVLE 185
              RGE  I + DA+F       G+G+++RD  G  +A +SS     SS  + E    LE
Sbjct: 277 TPKRGEFKI-NCDASFQLRSGKAGVGVIIRDYKGSAIAARSSPVLCCSSVEMLEAQACLE 336

Query: 186 GLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSFKFVSR 245
           G+ +A    V  ++I SD  ++I +++++    + +  +I     +      +S+  V R
Sbjct: 337 GIHLAIDIGVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGASVNLLSYVAVRR 396

Query: 246 KFNRFAHQTARVGLSSPSPILWQGNYP 270
           + N  AH  A+  LS  SP++W    P
Sbjct: 397 EANSVAHCIAQHALSLDSPVVWFEEMP 420

BLAST of Lag0014543 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 7.5e-28
Identity = 89/279 (31.90%), Postives = 131/279 (46.95%), Query Frame = 0

Query: 7    CHVCQEETETTKHALFQCSKARAIWELLLP----LMYDDQWGIVDIKDLWLGLTD-CQMS 66
            C +C +  E+  HA F C +AR IW  L P    L  +D    +   +LW  LT+  +  
Sbjct: 1173 CTICGDRRESIIHAFFHCKRARQIWRTLFPFLTCLSAEDN---ISFLELWSSLTEQLEPK 1232

Query: 67   DLERICVGAWAIWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGIP-IQSEKDI 126
            DL    +  W IWNDRNS IH   +     + +W+  +L    QA      P  QS    
Sbjct: 1233 DLNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHRP 1292

Query: 127  LEIISRGEETI---LHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPLVGEV 186
            +    R   ++   L+TDAA  G   +   G ++RD    L+A  S       SPL+ E+
Sbjct: 1293 VVQYWRPSSSVSLKLNTDAACRG--ASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEI 1352

Query: 187  IVVLEGLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSF 246
              +LEGL+ A A +   L + SD L  I +I  EI         + +I+ +   F  +SF
Sbjct: 1353 RCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISF 1412

Query: 247  KFVSRKFNRFAHQTARVGLSSPS-PILWQGNYPDWMVGL 276
               SR+ NR AH  A+ G++SPS    W  N+P W++ L
Sbjct: 1413 SHSSRQCNRAAHGLAKWGITSPSATYAWLFNFPTWLLDL 1446

BLAST of Lag0014543 vs. ExPASy TrEMBL
Match: A0A1R3HK95 (Zinc finger, CCHC-type OS=Corchorus olitorius OX=93759 GN=COLO4_28522 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.4e-21
Identity = 79/277 (28.52%), Postives = 122/277 (44.04%), Query Frame = 0

Query: 7   CHVCQEETETTKHALFQCSKARAIWELLLPLMYDDQWGIVDIKDLWLGLTDCQMSDLERI 66
           C  C +E ET  HA+ +C KA  +WEL+       +   VD     LG  D    D+   
Sbjct: 696 CIRCGKEEETMAHAIKECEKAEQVWELISEHYVASKNDEVDGSQTKLG--DLNWGDI--A 755

Query: 67  CVGAWAIWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGIPIQSEKDILEIISR 126
            V  WAIW  RN  IH++ ++     A ++  YL E+ +   K   P  +++   E   +
Sbjct: 756 LVTTWAIWGARNKEIHDNIVYSATETAGFVISYLMEYQRCKRKMQ-PNLAKETHWEAPGQ 815

Query: 127 GEETILHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPLVGEVIVVLEGLRM 186
            E  + + D A+     TG  G + RD  G +L   +      S   V E    L+  + 
Sbjct: 816 SEIKV-NFDGAYDSRGKTGAFGAIARDSAGLVLGAYAGRLGFVSDVYVAECKAALKAAKW 875

Query: 187 ARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSFKFVSRKFNR 246
           AR     R+++  D L +I   N      S I   I D+K  +N+FE  SF  V RK NR
Sbjct: 876 ARNMGFTRIILEGDALTVIKKANSSETDLSPITAYIADLKEAQNWFEKCSFSHVGRKGNR 935

Query: 247 FAHQTARVGLSSPSPILWQGNYPDWMVGLSLTECSLF 284
                A++G S    I+W  + P ++  +   +C+ F
Sbjct: 936 ATDVLAQLGKSYSESIIWMEDVPVYLSEIIQQDCTGF 966

BLAST of Lag0014543 vs. ExPASy TrEMBL
Match: A0A803QDG1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 2.6e-20
Identity = 71/265 (26.79%), Postives = 120/265 (45.28%), Query Frame = 0

Query: 7   CHVCQEETETTKHALFQCSKARAIWELLLPLMYDDQWGIVDIKDLWLGL-TDCQMSDLER 66
           C VC+ E ETTKHAL  CS+ R  W       Y D +  +DI   +LGL       D+  
Sbjct: 439 CPVCKMENETTKHALLDCSRVRKAWRYSRFADYYDAYKNLDIDGFFLGLFQTLDKHDIGI 498

Query: 67  ICVGAWAIWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGIPIQSEKDILEIIS 126
           +    W++WN RN+++HN          DW  ++  +F  A        QS      + S
Sbjct: 499 LFCFIWSLWNQRNNFVHNQYTVPPKEVFDWALVFYFKFLDARQHPITGSQSAPRSNSVQS 558

Query: 127 RGEETI-LHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPLVGEVIVVLEGL 186
                + L  DA          IGLV+ D    + A  S+ FS    P V E   + + +
Sbjct: 559 YSSIGLKLFIDATIDSINRRHSIGLVVLDADNAVKAGFSAPFSGLVPPAVAEAKAIFQAI 618

Query: 187 RMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSFKFVSRKF 246
             A+   +   ++L+D  ++++ +N     +S +  V+  I  + +F   ++  ++SR+ 
Sbjct: 619 HWAQLISLPVDVLLTDCKSIVDKLNSCNWNNSVLDDVLLSINNLLSFSPRLAISYISREN 678

Query: 247 NRFAHQTARVGLSSPSPILWQGNYP 270
           N  AH  A++GL   + ++W G+ P
Sbjct: 679 NVLAHNLAKLGLGLDNELVWNGSLP 703

BLAST of Lag0014543 vs. ExPASy TrEMBL
Match: A0A5C7H0P0 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 5.7e-20
Identity = 72/267 (26.97%), Postives = 124/267 (46.44%), Query Frame = 0

Query: 6   YCHVCQEETETTKHALFQCSKARAIWELLLPLMYDDQWGIVDIKDLWLGL---TDCQMSD 65
           YC VC +++E+  H L+ C+ A  +W  LL      +  + D   + L L    D  + +
Sbjct: 157 YCQVCNDDSESITHILWSCNSAVEVWRQLLGDDVIQRIVVSDFPSIILSLWRSVDSVVFN 216

Query: 66  LERICVGAWAIWNDRNSWIHNHPIHDTVSRADWINMYLTEFWQANPKGGIPIQSEKDILE 125
           L  + +G W +W +RNS +H            WI+ +  EF  AN      + S +   +
Sbjct: 217 L--LIIGYWRLWTNRNSVVHGSAGWAAADMVTWIDNFANEFRLANELNHKEVLSHQPSWK 276

Query: 126 IISRGEETILHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPLVGEVIVVLE 185
              RGE  I + DA+F       G+G+++RD  G  +A +SS     SS  + E    LE
Sbjct: 277 TPKRGEFKI-NCDASFQLRSGKAGVGVIIRDYKGSAIAARSSPVLCCSSVEMLEAQACLE 336

Query: 186 GLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSFKFVSR 245
           G+ +A    V  ++I SD  ++I +++++    + +  +I     +      +S+  V R
Sbjct: 337 GIHLAIDIGVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGASVNLLSYVAVRR 396

Query: 246 KFNRFAHQTARVGLSSPSPILWQGNYP 270
           + N  AH  A+  LS  SP++W    P
Sbjct: 397 EANSVAHCIAQHALSLDSPVVWFEEMP 420

BLAST of Lag0014543 vs. ExPASy TrEMBL
Match: A0A2P6QT88 (Putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0402201 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 7.5e-20
Identity = 74/279 (26.52%), Postives = 126/279 (45.16%), Query Frame = 0

Query: 1   MCWYGYCHVCQEETETTKHALFQCSKARAIWELLLPLMYDDQWGIVDIKDLWLG-LTDCQ 60
           +C    C  C E  ET  H L++C   R IW+L         W      DL+   L   Q
Sbjct: 296 ICSQSVCARCGEADETVIHCLWECRVVRKIWKLSWMWPVYKIWKEPSFMDLFAHVLASEQ 355

Query: 61  MSDLERICVGAWAIWNDRNSWIHNHPIHDTVSR----ADWINMYLTEFWQANP--KGGIP 120
              +E   +  W IW DRN++IH     D        +DW    L + W+     K  + 
Sbjct: 356 AEQVELFGLLIWWIWKDRNNFIHRQTSLDPSELLRKCSDW-QRELKQVWEKKQAHKHNVR 415

Query: 121 IQSEKDILEIISRGEETILHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSPL 180
            +++ ++       +   L+ D A   +    G+G V+R++LG+L+   +  F+    P 
Sbjct: 416 SKNQLEVKWERPPPQWCKLNFDGACEANGGNSGLGAVIRNELGELVGALAVPFAATLKPS 475

Query: 181 VGEVIVVLEGLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFE 240
             E + + EGLR +R   +R L +  D L +IN +++     + I  V+  ++++   FE
Sbjct: 476 AVEALALQEGLRYSRLLGIRNLEVEGDALCIINSLSQTENDLTEIGAVLEGVRLLLQEFE 535

Query: 241 NVSFKFVSRKFNRFAHQTARVGLSSPSPILWQGNYPDWM 273
            V++K V +K N+ AH+ AR  L S   +  +   P W+
Sbjct: 536 FVNWKHVRKKANQVAHELARSALKSVQSMFCRERGPPWL 573

BLAST of Lag0014543 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 6.5e-16
Identity = 74/280 (26.43%), Postives = 116/280 (41.43%), Query Frame = 0

Query: 7   CHVCQEETETTKHALFQCSKARAIWELLLPLMYDDQWGIVD----IKDLWLGLTDCQMSD 66
           C  C  E E+  HALF C  A   W L    +  +Q    D    I ++   + D  MSD
Sbjct: 200 CPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSNDFEENISNILNFVQDTTMSD 259

Query: 67  LERICVG--AWAIWNDRNSWIHN----HPIHDTVSRA----DWINMYLTEFWQANPKGGI 126
             ++      W IW  RN+ + N     P    +S      DW+N   +     +P   I
Sbjct: 260 FHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNATQSHKKTPSPTRQI 319

Query: 127 PIQSEKDILEIISRGEETILHTDAAFMGDRDTGGIGLVMRDKLGQLLAIKSSGFSNFSSP 186
              +E  I            + DA F   +     G ++R+  G  ++  S   ++ S+P
Sbjct: 320 ---AENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKLAHTSNP 379

Query: 187 LVGEVIVVLEGLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFF 246
           L  E   +L  L+        ++ +  D   LIN+IN  I  HSS+A  + DI    N F
Sbjct: 380 LEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLIN-GISFHSSLANHLEDISFWANKF 439

Query: 247 ENVSFKFVSRKFNRFAHQTARVGLSSPSPILWQGNYPDWM 273
            ++ F F+ RK N+ AH  A+ G +  +     G+ P W+
Sbjct: 440 ASIQFGFIRRKGNKLAHVLAKYGCTYSTFYSGSGSLPIWL 475

BLAST of Lag0014543 vs. TAIR 10
Match: AT1G10000.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 5.3e-10
Identity = 61/257 (23.74%), Postives = 105/257 (40.86%), Query Frame = 0

Query: 15  ETTKHALFQCSKARAIWELLLPLMYDDQWGIVDIKDLWLGLTDCQMSDLERICVG----- 74
           ET+ H LF C  A  +W  L PL    Q G + I    L   +     +    VG     
Sbjct: 40  ETSTHMLFHCDFAAQVWN-LAPL----QLGHIPIGTPILEALNLLKKTIVLPPVGIHTAT 99

Query: 75  -----AWAIWNDRNSWIHNH---PIHDTVSRADWINMYLTEFWQANPKGGIPIQSEKDIL 134
                 W IW  RN  I  +    + +TV++A    +       A PK  +         
Sbjct: 100 LFPWICWHIWKARNQLIFQNSHFSVIETVTKAVQDALAWQSAQLALPKVRVATPRPNSTT 159

Query: 135 EIISRGEETILHTDAAFMGDRDTGGIGLVMR--DKLGQLLAIKSSGFSNFSSPLVGEVIV 194
            +     + + + DAA+       G G V +      + +   S+G   F SPL  E   
Sbjct: 160 TLT---HDFLCYVDAAWQQQSSLAGSGWVFQATSHSEKEITTFSAGCRRFPSPLAAEAWA 219

Query: 195 VLEGLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSFKF 254
           +   +  A   +   L++LSD  ++++ +N  +   + I  ++ +I+ +RN F ++SF+F
Sbjct: 220 IKSAMLHALQLERSDLLVLSDSKSIVDALNSNV-SLNEIFGLLVEIRSIRNRFRSISFQF 279

Query: 255 VSRKFNRFAHQTARVGL 257
           + R  N  A   A++ L
Sbjct: 280 IPRLVNSIADAAAKLSL 287

BLAST of Lag0014543 vs. TAIR 10
Match: AT4G09490.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 1.2e-06
Identity = 41/132 (31.06%), Postives = 64/132 (48.48%), Query Frame = 0

Query: 128 EETILHTDAAFMGDRDTGGIGLVMRDKLGQLLAIK-SSGFSNFSSPLVGEVIVVLEGLRM 187
           +   + TDAA+  +    G G V+R+   +L A+   S   N   PL+ E I +   L+ 
Sbjct: 29  DRVTIFTDAAWKHETKDVGFGWVIRN-CPELTALHYQSAARNVRLPLMAEAIALFLALQY 88

Query: 188 ARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKVMRNFFENVSFKFVSRKFNR 247
           A++  + +L + SD   LI  I  E    +    +I+DI  +   F +VSF FV R  NR
Sbjct: 89  AQSIGITKLSMASDSQQLITAITSE-SPSTEFYGIIFDILNLSLGFADVSFSFVPRSENR 148

Query: 248 FAHQTARVGLSS 259
            A + A+  L S
Sbjct: 149 VADELAKSSLIS 158

BLAST of Lag0014543 vs. TAIR 10
Match: AT2G13980.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.4e-05
Identity = 28/91 (30.77%), Postives = 48/91 (52.75%), Query Frame = 0

Query: 168 NFSSPLVGEVIVVLEGLRMARAFDVRRLMILSDLLNLINIINEEIQGHSSIATVIWDIKV 227
           N S+PL  E   +L  L+        R+++  D   L N+++     H+ +A ++ DI++
Sbjct: 59  NTSTPLEAETKALLAALQQTWIRGYLRVIMEGDCETLTNLVSGS-SSHNHLANLLDDIRI 118

Query: 228 MRNFFENVSFKFVSRKFNRFAHQTARVGLSS 259
               F NV F FV R  N+ AH+ A++G +S
Sbjct: 119 WAKKFSNVQFSFVRRGGNKVAHELAKLGCNS 148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.11.5e-2731.90uncharacterized protein LOC111024874 [Momordica charantia][more]
OMO70721.12.8e-2128.52Zinc finger, CCHC-type [Corchorus olitorius][more]
XP_030508852.13.1e-2028.22uncharacterized protein LOC115723496 [Cannabis sativa][more]
XP_038704726.19.1e-2026.89uncharacterized protein LOC120000672 [Tripterygium wilfordii][more]
TXG50387.11.2e-1926.97hypothetical protein EZV62_022911 [Acer yangbiense][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DX307.5e-2831.90uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A1R3HK951.4e-2128.52Zinc finger, CCHC-type OS=Corchorus olitorius OX=93759 GN=COLO4_28522 PE=4 SV=1[more]
A0A803QDG12.6e-2026.79Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A5C7H0P05.7e-2026.97Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1[more]
A0A2P6QT887.5e-2026.52Putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-c... [more]
Match NameE-valueIdentityDescription
AT3G09510.16.5e-1626.43Ribonuclease H-like superfamily protein [more]
AT1G10000.15.3e-1023.74Ribonuclease H-like superfamily protein [more]
AT4G09490.11.2e-0631.06Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT2G13980.11.4e-0530.77Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 129..259
e-value: 8.4E-13
score: 50.4
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 134..253
e-value: 1.5E-20
score: 73.3
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 133..258
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 133..258
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 132..253
e-value: 2.07832E-17
score: 74.658
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 131..260

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014543.1Lag0014543.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity