Lag0013963 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0013963 vs. NCBI nr
Match: KAG6581394.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 648.7 bits (1672), Expect = 3.0e-182
Identity = 312/359 (86.91%), Postives = 331/359 (92.20%), Query Frame = 0

Query: 1   MEPGVTKLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQIPVIDMNNL 60
           MEP V KL +RD LPVPCVQELVKSSPSTVP RY+RPDQDPPF+FTD S Q+PVIDM+ L
Sbjct: 1   MEPQVMKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTSTQVPVIDMHKL 60

Query: 61  F-----EDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQK 120
                 ED ELDK HHACKDWGFFQLINHGVSDVLVESMKSGI+ALFNLPMEEK KLWQK
Sbjct: 61  LSSNNCEDPELDKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQK 120

Query: 121 PGDIEGFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKN 180
           PGD+EGFGQSFVVSEEQKLNWGDLFG+F  PTYLRKPHL  NLPLPFRDDLDAYALE+KN
Sbjct: 121 PGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFPNLPLPFRDDLDAYALEMKN 180

Query: 181 LAMKLLELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITIL 240
           LAMKLLELMAKALKM+ CEMREVFEEG++STRMNYYPPCPQPELVIGLNNHSDAS ITIL
Sbjct: 181 LAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITIL 240

Query: 241 LQVNEMEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLS 300
           LQVNEMEGLQI+KDGRW+PVKPLPNAFVVNIGDV+EIITNG YRSIEHRATVNSAKERLS
Sbjct: 241 LQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLS 300

Query: 301 VAIFYTPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQKE 355
           VA+FY+PRLDGEIGPAPSL+T ERPALFK IGV DFL GFF+RELNGKSYLDVMRIQKE
Sbjct: 301 VAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMRIQKE 359

BLAST of Lag0013963 vs. NCBI nr
Match: XP_023544158.1 (protein SRG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647.5 bits (1669), Expect = 6.7e-182
Identity = 312/359 (86.91%), Postives = 331/359 (92.20%), Query Frame = 0

Query: 1   MEPGVTKLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQIPVIDMNNL 60
           MEP V KL +RD LPVPCVQELVKSSPSTVP RY+RPDQDPPF FTD SAQ+PVID++ L
Sbjct: 1   MEPQVMKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFVFTDTSAQVPVIDLHKL 60

Query: 61  F-----EDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQK 120
                 ED ELDK HHACKDWGFFQLINHGVSDVLVESMKSGI+ALFNLPMEEK KLWQK
Sbjct: 61  LSSSNCEDPELDKFHHACKDWGFFQLINHGVSDVLVESMKSGIEALFNLPMEEKSKLWQK 120

Query: 121 PGDIEGFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKN 180
           PGD+EGFGQSFVVSEEQKLNWGDLFG+F  PTYLRKPHL  NLPLPFRDDLDAYALE+KN
Sbjct: 121 PGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFPNLPLPFRDDLDAYALEMKN 180

Query: 181 LAMKLLELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITIL 240
           LAMKLLELMAKALKM+ CEMREVFEEG++STRMNYYPPCPQPELVIGLNNHSDAS ITIL
Sbjct: 181 LAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITIL 240

Query: 241 LQVNEMEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLS 300
           LQVNEMEGLQI+KDGRW+PVKPLPNAFVVNIGDV+EIITNG YRSIEHRATVNSAKERLS
Sbjct: 241 LQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLS 300

Query: 301 VAIFYTPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQKE 355
           VA+FY+PRLDGEIGPAPSL+T ERPALFK IGV DFL GFF+RELNGKSYLDVMRIQKE
Sbjct: 301 VAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMRIQKE 359

BLAST of Lag0013963 vs. NCBI nr
Match: XP_022978724.1 (protein SRG1-like [Cucurbita maxima])

HSP 1 Score: 647.5 bits (1669), Expect = 6.7e-182
Identity = 311/359 (86.63%), Postives = 332/359 (92.48%), Query Frame = 0

Query: 1   MEPGVTKLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQIPVIDMNNL 60
           MEP + KL +RD LPVPCVQELVKSSPSTVP RY+RPDQDPPF+FTD SAQ+PVIDM+ L
Sbjct: 1   MEPQIMKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTSAQVPVIDMHKL 60

Query: 61  F-----EDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQK 120
                 ED ELDK HHACKDWGFFQLINHGVSDVLVESMKSGI+ALFNLPMEEK KLWQK
Sbjct: 61  LSSSNCEDPELDKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQK 120

Query: 121 PGDIEGFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKN 180
           PGD+EGFGQSFVVSEEQKLNWGDLFG+F  PTYLRKPHL  NLPLPFRDDLDAYALE+KN
Sbjct: 121 PGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFLNLPLPFRDDLDAYALEMKN 180

Query: 181 LAMKLLELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITIL 240
           LAMKLLELMAKALKM+ CEMREVFEEG++STRMNYYPPCPQPELVIGLNNHSDAS ITIL
Sbjct: 181 LAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITIL 240

Query: 241 LQVNEMEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLS 300
           LQVNEMEGLQI+KDGRW+PVKPLPNAFVVNIGDV+EIITNG YRSIEHRATVNSAKERLS
Sbjct: 241 LQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLS 300

Query: 301 VAIFYTPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQKE 355
           VA+FY+PRLDGEIGPAPSL+T ERPALFK IGV DFL GFF+RELNGKSYLDVM+IQKE
Sbjct: 301 VAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMKIQKE 359

BLAST of Lag0013963 vs. NCBI nr
Match: XP_022925780.1 (protein SRG1-like [Cucurbita moschata])

HSP 1 Score: 644.4 bits (1661), Expect = 5.7e-181
Identity = 310/359 (86.35%), Postives = 330/359 (91.92%), Query Frame = 0

Query: 1   MEPGVTKLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQIPVIDMNNL 60
           MEP V KL +RD LPVPCVQELVKSSPSTVP RY+RPDQDPPF+FTD S Q+PVIDM+ L
Sbjct: 1   MEPQVMKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTSTQVPVIDMHKL 60

Query: 61  F-----EDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQK 120
                 ED EL K HHACKDWGFFQLINHGVSDVLVESMKSGI+ALFNLPMEEK KLWQ+
Sbjct: 61  LSSNNCEDPELAKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQR 120

Query: 121 PGDIEGFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKN 180
           PGD+EGFGQSFVVSEEQKLNWGDLFG+F  PTYLRKPHL  NLPLPFRDDLDAYALE+KN
Sbjct: 121 PGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFPNLPLPFRDDLDAYALEMKN 180

Query: 181 LAMKLLELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITIL 240
           LAMKLLELMAKALKM+ CEMREVFEEG++STRMNYYPPCPQPELVIGLNNHSDAS ITIL
Sbjct: 181 LAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITIL 240

Query: 241 LQVNEMEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLS 300
           LQVNEMEGLQI+KDGRW+PVKPLPNAFVVNIGDV+EIITNG YRSIEHRATVNSAKERLS
Sbjct: 241 LQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLS 300

Query: 301 VAIFYTPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQKE 355
           VA+FY+PRLDGEIGPAPSL+T ERPALFK IGV DFL GFF+RELNGKSYLDVMRIQKE
Sbjct: 301 VAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMRIQKE 359

BLAST of Lag0013963 vs. NCBI nr
Match: KAG7034679.1 (Protein SRG1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 644.4 bits (1661), Expect = 5.7e-181
Identity = 310/359 (86.35%), Postives = 330/359 (91.92%), Query Frame = 0

Query: 1   MEPGVTKLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQIPVIDMNNL 60
           MEP V KL +RD LPVPCVQELVKSSPSTVP RY+RPDQDPPF+FTD S Q+PVIDM+ L
Sbjct: 362 MEPQVMKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTSTQVPVIDMHKL 421

Query: 61  F-----EDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQK 120
                 ED EL K HHACKDWGFFQLINHGVSDVLVESMKSGI+ALFNLPMEEK KLWQ+
Sbjct: 422 LSSNNCEDPELAKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQR 481

Query: 121 PGDIEGFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKN 180
           PGD+EGFGQSFVVSEEQKLNWGDLFG+F  PTYLRKPHL  NLPLPFRDDLDAYALE+KN
Sbjct: 482 PGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFPNLPLPFRDDLDAYALEMKN 541

Query: 181 LAMKLLELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITIL 240
           LAMKLLELMAKALKM+ CEMREVFEEG++STRMNYYPPCPQPELVIGLNNHSDAS ITIL
Sbjct: 542 LAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITIL 601

Query: 241 LQVNEMEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLS 300
           LQVNEMEGLQI+KDGRW+PVKPLPNAFVVNIGDV+EIITNG YRSIEHRATVNSAKERLS
Sbjct: 602 LQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLS 661

Query: 301 VAIFYTPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQKE 355
           VA+FY+PRLDGEIGPAPSL+T ERPALFK IGV DFL GFF+RELNGKSYLDVMRIQKE
Sbjct: 662 VAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMRIQKE 720

BLAST of Lag0013963 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 2.9e-111
Identity = 195/345 (56.52%), Postives = 256/345 (74.20%), Query Frame = 0

Query: 16  VPCVQELVKSSP-STVPLRYVRPDQDPP--FQFTDISAQIPVIDMNNLFE----DSELDK 75
           VP VQE+VK    +TVP RYVR DQD        D+  +IP+IDM  L      DSE++K
Sbjct: 14  VPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEK 73

Query: 76  LHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEGFGQSFVV 135
           L  ACK+WGFFQL+NHG+    ++ +KS I+  FNLPMEEK+K WQ+P +IEGFGQ+FVV
Sbjct: 74  LDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVV 133

Query: 136 SEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLLELMAKAL 195
           SE+QKL+W DLF     P  LRKPHL   LPLPFRD L+ Y+ E++++A  L+  MA+AL
Sbjct: 134 SEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARAL 193

Query: 196 KMDGCEMREVFE--EGIVSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNEMEGLQI 255
           ++   E+ ++F+  + + S RMNYYPPCPQP+ VIGL  HSD+ G+T+L+QVN++EGLQI
Sbjct: 194 EIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQI 253

Query: 256 KKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFYTPRLDG 315
           KKDG+W+PVKPLPNAF+VNIGDVLEIITNG YRSIEHR  VNS KERLS+A F+   +  
Sbjct: 254 KKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYK 313

Query: 316 EIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRI 352
           E+GPA SL+  ++ A FKR+ + ++ +G FSR L+GK+YLD +RI
Sbjct: 314 EVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Lag0013963 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 5.6e-107
Identity = 187/351 (53.28%), Postives = 254/351 (72.36%), Query Frame = 0

Query: 14  LPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQ------IPVIDMNNLFEDS--- 73
           L +P VQEL K + + +P RY    + P     +I A       +PVID+ NL       
Sbjct: 13  LSIPSVQELAKLTLAEIPSRYTCTGESP---LNNIGASVTDDETVPVIDLQNLLSPEPVV 72

Query: 74  ---ELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEG 133
              ELDKLH ACK+WGFFQL+NHGV  +L++++KS IK  FNLPM EK K  Q+ GD EG
Sbjct: 73  GKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEG 132

Query: 134 FGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLL 193
           FGQ ++ SE+Q+L+W ++F M + P +LRKPHL   LPLPFR+ L++Y  ++K L+  + 
Sbjct: 133 FGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTVVF 192

Query: 194 ELMAKALKMDGCE-MREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNE 253
           E++ K+L++   + M ++FE+G+ + RMNYYPPCP+PELV+GL +HSD SG+TILLQ+NE
Sbjct: 193 EMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQLNE 252

Query: 254 MEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFY 313
           +EGLQI+K+ RWI +KPLP+AF+VN+GD+LEI+TNGIYRS+EHRA VNS KERLS+A F+
Sbjct: 253 VEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIATFH 312

Query: 314 TPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRI 352
             +L+ EIGP  SL+T E PALFKR    D L    SR+L+GKS+LD MR+
Sbjct: 313 DSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

BLAST of Lag0013963 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 2.0e-104
Identity = 189/352 (53.69%), Postives = 246/352 (69.89%), Query Frame = 0

Query: 14  LPVPCVQELVKSSPSTVPLRYVRPDQDPPF----QFTDISAQIPVIDMNNLF------ED 73
           + +P VQEL K + + +P RY+   ++          D    +PVID+ NL       E 
Sbjct: 13  MSIPSVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISSEPVTEK 72

Query: 74  SELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEGFG 133
            ELD+LH ACK+WGFFQ++NHGV   LV+++KS I+  FNL M EK K  QK GD+EGFG
Sbjct: 73  LELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDGDVEGFG 132

Query: 134 QSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLLEL 193
           Q+FV SE+Q L+W D+F +   P +LRKPHL   LPLP R+ +++Y+ E+K L+M L E 
Sbjct: 133 QAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLSMVLFEK 192

Query: 194 MAKALKMDGCEMR---EVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNE 253
           M KAL++   E++   EVF++     RMNYYPPCPQPEL IGL  HSD  G+TILLQ+NE
Sbjct: 193 MEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTILLQLNE 252

Query: 254 MEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFY 313
           +EGLQIK +GRWI VKPLPNAFVVN+GDVLEI+TNG+YRS++HRA VNS KERLS+A F+
Sbjct: 253 VEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERLSIATFH 312

Query: 314 TPRLDGEIGPAPSLITSERPALFKRIGV-ADFLNGFFSRELNGKSYLDVMRI 352
            P L+ EIGP  SLIT   PALF+      + +  F SR+L+GKS+LD MR+
Sbjct: 313 DPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLDSMRM 364

BLAST of Lag0013963 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 3.8e-103
Identity = 189/352 (53.69%), Postives = 246/352 (69.89%), Query Frame = 0

Query: 14  LPVPCVQELVKSSPSTVPLRYVRPDQD--PPFQFTDIS--AQIPVIDMNNLFEDS----- 73
           + +P VQEL K + + +P RYV  +++   P   + I+    IPVID+ NL         
Sbjct: 13  MEIPSVQELAKLTLAEIPSRYVCANENLLLPMGASVINDHETIPVIDIENLLSPEPIIGK 72

Query: 74  -ELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEGFG 133
            ELD+LH ACK+WGFFQ++NHGV   LV+S+KS I+  FNL M+EK K  Q+ GD+EGFG
Sbjct: 73  LELDRLHFACKEWGFFQVVNHGVDASLVDSVKSEIQGFFNLSMDEKTKYEQEDGDVEGFG 132

Query: 134 QSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLLEL 193
           Q F+ SE+Q L+W D+F MF  P +LRKPHL   LP+P R+ +++Y+ E+K L+M L   
Sbjct: 133 QGFIESEDQTLDWADIFMMFTLPLHLRKPHLFSKLPVPLRETIESYSSEMKKLSMVLFNK 192

Query: 194 MAKALKMDGCE---MREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNE 253
           M KAL++   E   M EVF +G  + RMNYYPPCPQP L IGL +HSD  G+TILLQ+NE
Sbjct: 193 MEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPNLAIGLTSHSDFGGLTILLQINE 252

Query: 254 MEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFY 313
           +EGLQIK++G WI VKPLPNAFVVN+GD+LEI+TNGIY S++HRA VNS  ERLS+A F+
Sbjct: 253 VEGLQIKREGTWISVKPLPNAFVVNVGDILEIMTNGIYHSVDHRAVVNSTNERLSIATFH 312

Query: 314 TPRLDGEIGPAPSLITSERPALFKRIGV-ADFLNGFFSRELNGKSYLDVMRI 352
            P L+  IGP  SLIT E PALFK      D +    +R+L+GKS+LD MRI
Sbjct: 313 DPSLESVIGPISSLITPETPALFKSGSTYGDLVEECKTRKLDGKSFLDSMRI 364

BLAST of Lag0013963 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.2e-74
Identity = 140/349 (40.11%), Postives = 210/349 (60.17%), Query Frame = 0

Query: 14  LPVPCVQ---ELVKSSPSTVPLRYVRPDQDPPFQFTDISAQ--IPVIDMNNLF----EDS 73
           LPVP VQ   E+       +P RY+RP+        +      IP+ID+  L      + 
Sbjct: 10  LPVPNVQALAEICNDPDEHIPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQSSEE 69

Query: 74  ELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEGFGQ 133
           E  KL  AC+ WGFF LINHGV D ++ ++K  I   F+ P++ K++  Q P  +EG+GQ
Sbjct: 70  ECVKLRSACQYWGFFLLINHGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQ 129

Query: 134 SFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLLELM 193
           SFV SE+QKL+W D+  +  HP+  R        P  FR  +DAY+ E K+LA+ L E M
Sbjct: 130 SFVFSEDQKLDWADMLYLHVHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFM 189

Query: 194 AKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNEMEGL 253
           AKA+      + ++FEE     RM YYPPC Q + V+GL+ HSDA G+T+LL++N ++GL
Sbjct: 190 AKAVGAKPESLLDLFEEQPRGLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGL 249

Query: 254 QIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFYTPRL 313
           QIKKDG+W  +     A + NIGD LEI++NG +RS+EHRA +N  KER+S A+F+ P  
Sbjct: 250 QIKKDGKWFSIDAPNGALIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSE 309

Query: 314 DGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQK 354
           +  I P P  +  +    ++ I   DF+   F+++L+GK+ ++V+++ +
Sbjct: 310 NMVISPLPEFV-KDGKVKYRSISYLDFMKQIFTQQLDGKNRVEVLKLDQ 357

BLAST of Lag0013963 vs. ExPASy TrEMBL
Match: A0A6J1IUU4 (protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111478595 PE=3 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 3.2e-182
Identity = 311/359 (86.63%), Postives = 332/359 (92.48%), Query Frame = 0

Query: 1   MEPGVTKLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQIPVIDMNNL 60
           MEP + KL +RD LPVPCVQELVKSSPSTVP RY+RPDQDPPF+FTD SAQ+PVIDM+ L
Sbjct: 1   MEPQIMKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTSAQVPVIDMHKL 60

Query: 61  F-----EDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQK 120
                 ED ELDK HHACKDWGFFQLINHGVSDVLVESMKSGI+ALFNLPMEEK KLWQK
Sbjct: 61  LSSSNCEDPELDKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQK 120

Query: 121 PGDIEGFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKN 180
           PGD+EGFGQSFVVSEEQKLNWGDLFG+F  PTYLRKPHL  NLPLPFRDDLDAYALE+KN
Sbjct: 121 PGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFLNLPLPFRDDLDAYALEMKN 180

Query: 181 LAMKLLELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITIL 240
           LAMKLLELMAKALKM+ CEMREVFEEG++STRMNYYPPCPQPELVIGLNNHSDAS ITIL
Sbjct: 181 LAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITIL 240

Query: 241 LQVNEMEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLS 300
           LQVNEMEGLQI+KDGRW+PVKPLPNAFVVNIGDV+EIITNG YRSIEHRATVNSAKERLS
Sbjct: 241 LQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLS 300

Query: 301 VAIFYTPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQKE 355
           VA+FY+PRLDGEIGPAPSL+T ERPALFK IGV DFL GFF+RELNGKSYLDVM+IQKE
Sbjct: 301 VAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMKIQKE 359

BLAST of Lag0013963 vs. ExPASy TrEMBL
Match: A0A6J1EJ61 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433085 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.7e-181
Identity = 310/359 (86.35%), Postives = 330/359 (91.92%), Query Frame = 0

Query: 1   MEPGVTKLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQIPVIDMNNL 60
           MEP V KL +RD LPVPCVQELVKSSPSTVP RY+RPDQDPPF+FTD S Q+PVIDM+ L
Sbjct: 1   MEPQVMKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTSTQVPVIDMHKL 60

Query: 61  F-----EDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQK 120
                 ED EL K HHACKDWGFFQLINHGVSDVLVESMKSGI+ALFNLPMEEK KLWQ+
Sbjct: 61  LSSNNCEDPELAKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQR 120

Query: 121 PGDIEGFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKN 180
           PGD+EGFGQSFVVSEEQKLNWGDLFG+F  PTYLRKPHL  NLPLPFRDDLDAYALE+KN
Sbjct: 121 PGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFPNLPLPFRDDLDAYALEMKN 180

Query: 181 LAMKLLELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITIL 240
           LAMKLLELMAKALKM+ CEMREVFEEG++STRMNYYPPCPQPELVIGLNNHSDAS ITIL
Sbjct: 181 LAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITIL 240

Query: 241 LQVNEMEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLS 300
           LQVNEMEGLQI+KDGRW+PVKPLPNAFVVNIGDV+EIITNG YRSIEHRATVNSAKERLS
Sbjct: 241 LQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLS 300

Query: 301 VAIFYTPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQKE 355
           VA+FY+PRLDGEIGPAPSL+T ERPALFK IGV DFL GFF+RELNGKSYLDVMRIQKE
Sbjct: 301 VAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMRIQKE 359

BLAST of Lag0013963 vs. ExPASy TrEMBL
Match: A0A5A7UBP9 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008610 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 7.0e-177
Identity = 302/359 (84.12%), Postives = 330/359 (91.92%), Query Frame = 0

Query: 1   MEPGVTKLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQIPVIDMNNL 60
           ME   +KL +RD LPVPCVQEL KSS STVPLRYVRPDQDPPF+FTD SA++PVIDM+ L
Sbjct: 43  METLASKLQLRDSLPVPCVQELAKSSLSTVPLRYVRPDQDPPFEFTDASAEVPVIDMHKL 102

Query: 61  -----FEDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQK 120
                F+DSELDKLHHACKDWGFFQ+INHGVSDVLVE++KSGI+ALFNLPM EKRKLWQ+
Sbjct: 103 LFSNNFDDSELDKLHHACKDWGFFQVINHGVSDVLVENVKSGIQALFNLPMVEKRKLWQR 162

Query: 121 PGDIEGFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKN 180
           PGD+EGFGQSFVVSEEQKLNWGDLFGMF  PTYLRKPHL  NLPLPFRDDLDAYALEIKN
Sbjct: 163 PGDVEGFGQSFVVSEEQKLNWGDLFGMFILPTYLRKPHLFPNLPLPFRDDLDAYALEIKN 222

Query: 181 LAMKLLELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITIL 240
           L MKL +LMAKAL+MD  EMRE++EEG++STRMNYYPPCPQPELV+GLNNHSDAS ITIL
Sbjct: 223 LGMKLFDLMAKALEMDSSEMRELYEEGMLSTRMNYYPPCPQPELVMGLNNHSDASAITIL 282

Query: 241 LQVNEMEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLS 300
           LQVNEMEGLQI+KDGRW PVKPLPNAFVVNIGDVLEI+TNGIYRSIEHRATVNS KERLS
Sbjct: 283 LQVNEMEGLQIRKDGRWTPVKPLPNAFVVNIGDVLEIVTNGIYRSIEHRATVNSTKERLS 342

Query: 301 VAIFYTPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQKE 355
           VA+F++PRLDGEIGPAPSL+TSER ALFKRIGVADFLN FF RELNG+SYLDVMRIQ+E
Sbjct: 343 VAMFFSPRLDGEIGPAPSLVTSERSALFKRIGVADFLNEFFKRELNGRSYLDVMRIQEE 401

BLAST of Lag0013963 vs. ExPASy TrEMBL
Match: A0A6J1EL63 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433603 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.6e-168
Identity = 291/362 (80.39%), Postives = 318/362 (87.85%), Query Frame = 0

Query: 1   MEPGVTKLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDISAQIPVIDMNNL 60
           ME   T L + D L VP VQEL KSSPSTVPLRYVRP+QDPPFQF D S QIPVID++ L
Sbjct: 1   MEQEATMLRLGDSLLVPSVQELAKSSPSTVPLRYVRPNQDPPFQFIDTSPQIPVIDLHKL 60

Query: 61  F-----EDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQK 120
                 +D EL KLH ACKDWGFFQLINHGVSD LVE+MKSG++ALFNLP+EEKRKLWQK
Sbjct: 61  LFSNNSQDCELHKLHLACKDWGFFQLINHGVSDALVENMKSGVEALFNLPLEEKRKLWQK 120

Query: 121 PGDIEGFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKN 180
           PGD EGFGQSFV SEEQKLNWGDLF +F  P YLRKPHL  NLPLPFRDDLDAYALE++ 
Sbjct: 121 PGDFEGFGQSFVFSEEQKLNWGDLFAVFILPAYLRKPHLFPNLPLPFRDDLDAYALEMEI 180

Query: 181 LAMKLLELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITIL 240
           LAMKLL+LMAK LKM+GCEMRE+   GIVSTRMNYYPPCPQPELVIGLNNHSDASG+TIL
Sbjct: 181 LAMKLLDLMAKTLKMEGCEMRELLGGGIVSTRMNYYPPCPQPELVIGLNNHSDASGLTIL 240

Query: 241 LQVNEMEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLS 300
           LQVNEMEGLQI+KDGRWIPV+PLPNAFVVNIGD+LEI+TNGIYRSIEHR TVNSAKERLS
Sbjct: 241 LQVNEMEGLQIRKDGRWIPVQPLPNAFVVNIGDILEIVTNGIYRSIEHRVTVNSAKERLS 300

Query: 301 VAIFYTPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQKEE 358
           +AIFY+PRLDGEIGPAPSL++S+ PALFKRIGV DFL GFFS+ELNGKSYLD MRIQ E 
Sbjct: 301 LAIFYSPRLDGEIGPAPSLVSSDSPALFKRIGVTDFLKGFFSKELNGKSYLDCMRIQNEG 360

BLAST of Lag0013963 vs. ExPASy TrEMBL
Match: A0A6J1CMB4 (protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111012287 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.8e-161
Identity = 279/352 (79.26%), Postives = 307/352 (87.22%), Query Frame = 0

Query: 7   KLHVRDYLPVPCVQELVKSSPSTVPLRYVRPDQDPPFQFTDIS-AQIPVIDMNNLF---- 66
           +L + D L VPCV+EL K   STVPLRYVR DQDPPF+F D S AQIPVIDM+ L     
Sbjct: 2   ELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSDH 61

Query: 67  -EDSELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIE 126
            EDSELDKLHHACKDWGFFQLINHGV+D LVESM+SGI+ LF+LPMEEKRKLWQK GD E
Sbjct: 62  SEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFE 121

Query: 127 GFGQSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKL 186
           GFGQ+FVVSEEQKLNWGDLFG+   PTYLRKPHL  NLPLPFRDDLD YA E+K LAMKL
Sbjct: 122 GFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKL 181

Query: 187 LELMAKALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNE 246
           L+ MAKALKM+GCEMRE+FEEG+   RMNYYPPCPQPELVIGLNNHSDASGITILLQ+NE
Sbjct: 182 LDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLNE 241

Query: 247 MEGLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFY 306
           MEGLQI+ DGRWIPVKPLPNAFVVN+GDV+EI+TNGIY SIEHRA VNS KERLSVA+FY
Sbjct: 242 MEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFY 301

Query: 307 TPRLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQ 353
            PRLDGE+GPAPSL+T+ERP LFKRI V D+  GFFS+ELNGKSYLDVMRI+
Sbjct: 302 NPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIE 353

BLAST of Lag0013963 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 403.3 bits (1035), Expect = 2.0e-112
Identity = 195/345 (56.52%), Postives = 256/345 (74.20%), Query Frame = 0

Query: 16  VPCVQELVKSSP-STVPLRYVRPDQDPP--FQFTDISAQIPVIDMNNLFE----DSELDK 75
           VP VQE+VK    +TVP RYVR DQD        D+  +IP+IDM  L      DSE++K
Sbjct: 14  VPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEK 73

Query: 76  LHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEGFGQSFVV 135
           L  ACK+WGFFQL+NHG+    ++ +KS I+  FNLPMEEK+K WQ+P +IEGFGQ+FVV
Sbjct: 74  LDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVV 133

Query: 136 SEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLLELMAKAL 195
           SE+QKL+W DLF     P  LRKPHL   LPLPFRD L+ Y+ E++++A  L+  MA+AL
Sbjct: 134 SEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARAL 193

Query: 196 KMDGCEMREVFE--EGIVSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNEMEGLQI 255
           ++   E+ ++F+  + + S RMNYYPPCPQP+ VIGL  HSD+ G+T+L+QVN++EGLQI
Sbjct: 194 EIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQI 253

Query: 256 KKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFYTPRLDG 315
           KKDG+W+PVKPLPNAF+VNIGDVLEIITNG YRSIEHR  VNS KERLS+A F+   +  
Sbjct: 254 KKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYK 313

Query: 316 EIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRI 352
           E+GPA SL+  ++ A FKR+ + ++ +G FSR L+GK+YLD +RI
Sbjct: 314 EVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Lag0013963 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 399.8 bits (1026), Expect = 2.3e-111
Identity = 202/344 (58.72%), Postives = 249/344 (72.38%), Query Frame = 0

Query: 16  VPCVQELVKSSPST--VPLRYVRPDQDPPFQFTDISA-QIPVIDMNNLFE----DSELDK 75
           VP VQE+VK    T  +P RYVR DQ+      D    QIP+IDM+ L      DSE+DK
Sbjct: 13  VPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDMSLLSSSTSMDSEIDK 72

Query: 76  LHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEGFGQSFVV 135
           L  ACK+WGFFQL+NHG+    ++  KS I+  FNLPMEEK+KLWQ+PGDIEGFGQ+FV 
Sbjct: 73  LDFACKEWGFFQLVNHGMD---LDKFKSDIQDFFNLPMEEKKKLWQQPGDIEGFGQAFVF 132

Query: 136 SEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLLELMAKAL 195
           SEEQKL+W D+F +   P  LRKPHL   LPLPFRD LD Y+ E+K++A  L   +A AL
Sbjct: 133 SEEQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELKSIAKVLFAKLASAL 192

Query: 196 KMDGCEMREVFEEGI-VSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNEMEGLQIK 255
           K+   EM ++F++ +    RMNYYPPCP+P+  IGL  HSDA+G+TILLQVNE+EGLQIK
Sbjct: 193 KIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQVNEVEGLQIK 252

Query: 256 KDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFYTPRLDGE 315
           KDG+W+ VKPLPNA VVN+GD+LEIITNG YRSIEHR  VNS KERLSVA F+      E
Sbjct: 253 KDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVASFHNTGFGKE 312

Query: 316 IGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRI 352
           IGP  SL+   + ALFK +   ++ +G FSREL+GK+YLDVMRI
Sbjct: 313 IGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

BLAST of Lag0013963 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 399.4 bits (1025), Expect = 3.0e-111
Identity = 201/346 (58.09%), Postives = 252/346 (72.83%), Query Frame = 0

Query: 16  VPCVQELVKSSP-STVPLRYVRPDQDPP---FQFTDISAQIPVIDMNNLFE----DSELD 75
           VP VQE+VK    +TVP RYVR DQD        + + ++IP+IDMN L      DSE++
Sbjct: 14  VPSVQEMVKDKMITTVPPRYVRYDQDKTEVVVHDSGLISEIPIIDMNRLCSSTAVDSEVE 73

Query: 76  KLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEGFGQSFV 135
           KL  ACK++GFFQL+NHG+    ++ +KS I+  FNLPMEEK+KLWQ P  +EGFGQ+FV
Sbjct: 74  KLDFACKEYGFFQLVNHGIDPSFLDKIKSEIQDFFNLPMEEKKKLWQTPAVMEGFGQAFV 133

Query: 136 VSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLLELMAKA 195
           VSE+QKL+W DLF +   P  LRK HL   LPLPFRD LD Y+  +K++A  LL  MAKA
Sbjct: 134 VSEDQKLDWADLFFLIMQPVQLRKRHLFPKLPLPFRDTLDMYSTRVKSIAKILLAKMAKA 193

Query: 196 LKMDGCEMREVFEEGIV-STRMNYYPPCPQPELVIGLNNHSDASGITILLQVNEMEGLQI 255
           L++   E+ E+F + ++ S RMNYYPPCPQP LV GL  HSDA G+TILLQVNE++GLQI
Sbjct: 194 LQIKPEEVEEIFGDDMMQSMRMNYYPPCPQPNLVTGLIPHSDAVGLTILLQVNEVDGLQI 253

Query: 256 KKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFYTPRLDG 315
           KK+G+W  VKPL NAF+VN+GDVLEIITNG YRSIEHRA VN  KERLS+A F+   +D 
Sbjct: 254 KKNGKWFFVKPLQNAFIVNVGDVLEIITNGTYRSIEHRAMVNLEKERLSIATFHNTGMDK 313

Query: 316 EIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRIQ 353
           EIGPA SL+  +  A F+ +   D+LNG FSREL GK+YLD MRI+
Sbjct: 314 EIGPARSLVQRQEAAKFRSLKTKDYLNGLFSRELKGKAYLDAMRIE 359

BLAST of Lag0013963 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 394.8 bits (1013), Expect = 7.3e-110
Identity = 198/349 (56.73%), Postives = 250/349 (71.63%), Query Frame = 0

Query: 11  RDYLPVPCVQELVKSSP-STVPLRYVRPDQDPPFQFTD--ISAQIPVIDMNNLFE----D 70
           R  + VP VQE+VK    +TVP RYVR DQD      D  +  QIP+IDM+ L      D
Sbjct: 8   RSSIIVPSVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDMSLLCSSTSMD 67

Query: 71  SELDKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEGFG 130
           SE+DKL  ACK+WGFFQL+NHG+    +  +KS ++  FNLPMEEK+ LWQ+P +IEGFG
Sbjct: 68  SEIDKLDSACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGFG 127

Query: 131 QSFVVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLLEL 190
           Q FVVSEEQKL+W D+F +   P  LRKPHL   LPLPFRD LD Y+ E+K++A  LL  
Sbjct: 128 QVFVVSEEQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKILLGK 187

Query: 191 MAKALKMDGCEMREVFEEGI-VSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNEME 250
           +A ALK+   EM ++F++ +    R+NYYP CP+P+ VIGL  HSD++G+TILLQ NE+E
Sbjct: 188 IAVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQANEVE 247

Query: 251 GLQIKKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFYTP 310
           GLQIKK+ +W+ VKPLPNA VVN+GD+LEIITNG YRSIEHR  VNS KERLSVA F+  
Sbjct: 248 GLQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAFHNI 307

Query: 311 RLDGEIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRI 352
            L  EIGP  SL+   + A FK +   ++ NG FSREL+GK+YLDVMR+
Sbjct: 308 GLGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

BLAST of Lag0013963 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 376.3 bits (965), Expect = 2.7e-104
Identity = 183/345 (53.04%), Postives = 249/345 (72.17%), Query Frame = 0

Query: 14  LPVPCVQELVKSSP-STVPLRYVRPDQDPPFQFTD--ISAQIPVIDMNNLFE----DSEL 73
           L VP V E+VK    +T+P RYVR DQ+      D  +S++IPVIDM  L      DSEL
Sbjct: 12  LIVPFVLEIVKEKNFTTIPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLCSVSAMDSEL 71

Query: 74  DKLHHACKDWGFFQLINHGVSDVLVESMKSGIKALFNLPMEEKRKLWQKPGDIEGFGQSF 133
            KL  AC+DWGFFQL+NHG+    +E +++ ++  FNLPM+EK+KLWQ+ G+ EGFGQ  
Sbjct: 72  KKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEGFGQVN 131

Query: 134 VVSEEQKLNWGDLFGMFNHPTYLRKPHLVDNLPLPFRDDLDAYALEIKNLAMKLLELMAK 193
           +VSE QKL+WGD+F +   P   RK HL   LP PFR+ L+ Y+ E+K++A  L   MA 
Sbjct: 132 IVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILFAKMAS 191

Query: 194 ALKMDGCEMREVFEEGIVSTRMNYYPPCPQPELVIGLNNHSDASGITILLQVNEMEGLQI 253
            L++   EM ++F++   S ++NYYPPCPQP+ V+GL  HSDA+G+TILLQVN++EGLQI
Sbjct: 192 VLEIKHEEMEDLFDDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQVEGLQI 251

Query: 254 KKDGRWIPVKPLPNAFVVNIGDVLEIITNGIYRSIEHRATVNSAKERLSVAIFYTPRLDG 313
           KKDG+W+ VKPL +A VVN+G++LEIITNG YRSIEHRA VNS KERLSVA+F++P  + 
Sbjct: 252 KKDGKWVVVKPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFHSPGKET 311

Query: 314 EIGPAPSLITSERPALFKRIGVADFLNGFFSRELNGKSYLDVMRI 352
            I PA SL+  ++  LFK +   ++ + FF+++LNGKS+LD+MRI
Sbjct: 312 IIRPAKSLVDRQKQCLFKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356