Lag0013913 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013913
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCBL-interacting serine/threonine-protein kinase 12
Locationchr1: 53677178 .. 53678581 (-)
RNA-Seq ExpressionLag0013913
SyntenyLag0013913
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGACCGGCCGGACCCCGACAAGAAGACTGAAACCACCAGGAAGGACATGCCGGCTCTTCTTTTGGGTCGATACGAGATCGGGAAGCTCCTTGGCCATGGCACCTTCGCTAAGGTCTACCATGCCCGCAACATCAAAACCAACGAAAGCGTCGCCATTAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGCCTTATCGCCCACATCAAACGCGAGATCTCAATCCTCCGCCGCGTTCGCCACCCCAATATTGTGCAACTCTTCGAGGTTATGGCCACCAAGGCCAAGATCTACTTTGTTATGGAATATGTCCGAGGCGGCGAGCTTTTCAAGAAGGTCTCCAAGGGCCGATTGAAGGAAGAGGTCGCTCGAAAGTACTTTCAGCAATTAATCTCTGCTGTTGGATTTTGCCACGCGCGTGGTGTTTATCATCGCGACCTTAAACCGGAGAATTTGTTGCTCGATGAAAATGGGAATCTGAAGGTCTCCGATTTTGGCCTCAGCGCCGTCTCCGATCAAATTCGACAGGATGGGTTGTTTCATACTTTCTGTGGTACACCGGCGTATGTTGCTCCGGAGGTTTTGGCCCGAAAAGGGTATGAGGCCGCTAAGGTCGATATTTGGTCTTGTGGGGTTATTCTGTTTGTTCTGATGGCCGGTTATTTGCCCTTCCATGACCAAAATATTATGGCAATGTATAAGAAGATTTACAAGGGGGAGTTTCGGTGTCCGAGATGGTTCTCACCGGAGCTAATTCGGCTTCTAACTCGCCTGTTAGACACAAATCCCGAAACCCGTTTCACAATTCCTGAAATCATGGAGAATAGATGGTTCAAAAAGGGGTATAAACATATCAAATTCTACATCGAAGATGATAAGGTCTGCAGCGTTGAGGATGACAACGATGATGTCGATTCCTTGTCGGATCAATCTCTATCGGAATCGGATTCCGAAATTATTGAGACCAGAAGAAGGCCGGGTTCACTCCCAAGACCGGCGAGTTTGAATGCATTTGATATCATTTCGTTTTCGCCTGGATTTGATCTTTCTGGGTTGTTTGAGGATGGAGGGGAAGAGGCTAGATTTGTCTCCAGTGCTCCGGTGTCAAAGATTATATCGAAATTGGAGGAGATTGCAAAATTGGTGAGTTTCACTGTGAGGAAGAAGGATTGCAGGGTTAGTTTAGAGGGCTCACGGGAAGGTGTAAAAGGGCCATTGACGATCGCGGCCGAGGTATTCGAGTTGACGCCGAAATTGGTGATGGTGGAGGTGAAGAGGAAAGGGGGAGATAAAGCAGAGTATGAACAGTTCTGTAACAAGGAATTGAAACCAGCATTGTTGAATCTAAAAGTGGAGGACTCTGCTGCTGCAGCACATATACAATCAGATACTGAATGA

mRNA sequence

ATGGCCGACCGGCCGGACCCCGACAAGAAGACTGAAACCACCAGGAAGGACATGCCGGCTCTTCTTTTGGGTCGATACGAGATCGGGAAGCTCCTTGGCCATGGCACCTTCGCTAAGGTCTACCATGCCCGCAACATCAAAACCAACGAAAGCGTCGCCATTAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGCCTTATCGCCCACATCAAACGCGAGATCTCAATCCTCCGCCGCGTTCGCCACCCCAATATTGTGCAACTCTTCGAGGTTATGGCCACCAAGGCCAAGATCTACTTTGTTATGGAATATGTCCGAGGCGGCGAGCTTTTCAAGAAGGTCTCCAAGGGCCGATTGAAGGAAGAGGTCGCTCGAAAGTACTTTCAGCAATTAATCTCTGCTGTTGGATTTTGCCACGCGCGTGGTGTTTATCATCGCGACCTTAAACCGGAGAATTTGTTGCTCGATGAAAATGGGAATCTGAAGGTCTCCGATTTTGGCCTCAGCGCCGTCTCCGATCAAATTCGACAGGATGGGTTGTTTCATACTTTCTGTGGTACACCGGCGTATGTTGCTCCGGAGGTTTTGGCCCGAAAAGGGTATGAGGCCGCTAAGGTCGATATTTGGTCTTGTGGGGTTATTCTGTTTGTTCTGATGGCCGGTTATTTGCCCTTCCATGACCAAAATATTATGGCAATGTATAAGAAGATTTACAAGGGGGAGTTTCGGTGTCCGAGATGGTTCTCACCGGAGCTAATTCGGCTTCTAACTCGCCTGTTAGACACAAATCCCGAAACCCGTTTCACAATTCCTGAAATCATGGAGAATAGATGGTTCAAAAAGGGGTATAAACATATCAAATTCTACATCGAAGATGATAAGGTCTGCAGCGTTGAGGATGACAACGATGATGTCGATTCCTTGTCGGATCAATCTCTATCGGAATCGGATTCCGAAATTATTGAGACCAGAAGAAGGCCGGGTTCACTCCCAAGACCGGCGAGTTTGAATGCATTTGATATCATTTCGTTTTCGCCTGGATTTGATCTTTCTGGGTTGTTTGAGGATGGAGGGGAAGAGGCTAGATTTGTCTCCAGTGCTCCGGTGTCAAAGATTATATCGAAATTGGAGGAGATTGCAAAATTGGTGAGTTTCACTGTGAGGAAGAAGGATTGCAGGGTTAGTTTAGAGGGCTCACGGGAAGGTGTAAAAGGGCCATTGACGATCGCGGCCGAGGTATTCGAGTTGACGCCGAAATTGGTGATGGTGGAGGTGAAGAGGAAAGGGGGAGATAAAGCAGAGTATGAACAGTTCTGTAACAAGGAATTGAAACCAGCATTGTTGAATCTAAAAGTGGAGGACTCTGCTGCTGCAGCACATATACAATCAGATACTGAATGA

Coding sequence (CDS)

ATGGCCGACCGGCCGGACCCCGACAAGAAGACTGAAACCACCAGGAAGGACATGCCGGCTCTTCTTTTGGGTCGATACGAGATCGGGAAGCTCCTTGGCCATGGCACCTTCGCTAAGGTCTACCATGCCCGCAACATCAAAACCAACGAAAGCGTCGCCATTAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGCCTTATCGCCCACATCAAACGCGAGATCTCAATCCTCCGCCGCGTTCGCCACCCCAATATTGTGCAACTCTTCGAGGTTATGGCCACCAAGGCCAAGATCTACTTTGTTATGGAATATGTCCGAGGCGGCGAGCTTTTCAAGAAGGTCTCCAAGGGCCGATTGAAGGAAGAGGTCGCTCGAAAGTACTTTCAGCAATTAATCTCTGCTGTTGGATTTTGCCACGCGCGTGGTGTTTATCATCGCGACCTTAAACCGGAGAATTTGTTGCTCGATGAAAATGGGAATCTGAAGGTCTCCGATTTTGGCCTCAGCGCCGTCTCCGATCAAATTCGACAGGATGGGTTGTTTCATACTTTCTGTGGTACACCGGCGTATGTTGCTCCGGAGGTTTTGGCCCGAAAAGGGTATGAGGCCGCTAAGGTCGATATTTGGTCTTGTGGGGTTATTCTGTTTGTTCTGATGGCCGGTTATTTGCCCTTCCATGACCAAAATATTATGGCAATGTATAAGAAGATTTACAAGGGGGAGTTTCGGTGTCCGAGATGGTTCTCACCGGAGCTAATTCGGCTTCTAACTCGCCTGTTAGACACAAATCCCGAAACCCGTTTCACAATTCCTGAAATCATGGAGAATAGATGGTTCAAAAAGGGGTATAAACATATCAAATTCTACATCGAAGATGATAAGGTCTGCAGCGTTGAGGATGACAACGATGATGTCGATTCCTTGTCGGATCAATCTCTATCGGAATCGGATTCCGAAATTATTGAGACCAGAAGAAGGCCGGGTTCACTCCCAAGACCGGCGAGTTTGAATGCATTTGATATCATTTCGTTTTCGCCTGGATTTGATCTTTCTGGGTTGTTTGAGGATGGAGGGGAAGAGGCTAGATTTGTCTCCAGTGCTCCGGTGTCAAAGATTATATCGAAATTGGAGGAGATTGCAAAATTGGTGAGTTTCACTGTGAGGAAGAAGGATTGCAGGGTTAGTTTAGAGGGCTCACGGGAAGGTGTAAAAGGGCCATTGACGATCGCGGCCGAGGTATTCGAGTTGACGCCGAAATTGGTGATGGTGGAGGTGAAGAGGAAAGGGGGAGATAAAGCAGAGTATGAACAGTTCTGTAACAAGGAATTGAAACCAGCATTGTTGAATCTAAAAGTGGAGGACTCTGCTGCTGCAGCACATATACAATCAGATACTGAATGA

Protein sequence

MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPKLVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAAAAHIQSDTE
Homology
BLAST of Lag0013913 vs. NCBI nr
Match: XP_008439731.1 (PREDICTED: CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo] >KAA0052614.1 CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo var. makuwa] >TYK13214.1 CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo var. makuwa])

HSP 1 Score: 905.6 bits (2339), Expect = 1.8e-259
Identity = 456/467 (97.64%), Postives = 458/467 (98.07%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDNDDVDSLSDQS SESDSEIIETRR+  SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420
           EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAAAAHIQSDTE 468
           LVMVEVKRKGGDKAEYEQFCN ELKPALLNLKVEDS   +HI SDTE
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Lag0013913 vs. NCBI nr
Match: XP_038882513.1 (CBL-interacting serine/threonine-protein kinase 12-like [Benincasa hispida])

HSP 1 Score: 904.8 bits (2337), Expect = 3.0e-259
Identity = 455/467 (97.43%), Postives = 459/467 (98.29%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDN+DVDSLSDQS SESDSEI ETRR+  SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNNDVDSLSDQSQSESDSEIFETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420
           EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGV+GPLTIAAEVFELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVRGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAAAAHIQSDTE 468
           LVMVEVKRKGGDKAEYEQFCN ELKPALLNLKVEDSAA +HI SDTE
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSDTE 467

BLAST of Lag0013913 vs. NCBI nr
Match: XP_011658253.1 (CBL-interacting serine/threonine-protein kinase 12 [Cucumis sativus] >KGN49312.1 hypothetical protein Csa_003581 [Cucumis sativus])

HSP 1 Score: 899.8 bits (2324), Expect = 9.8e-258
Identity = 452/467 (96.79%), Postives = 456/467 (97.64%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTN+SVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNDSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCS+E
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDVDSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DD DDVDSLSDQS SESDSEIIETRR+  SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDTDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420
           EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAAAAHIQSDTE 468
           LVMVEVKRKGGDK EYEQFCN ELKPALLNLKVEDS   +HI SDTE
Sbjct: 421 LVMVEVKRKGGDKEEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Lag0013913 vs. NCBI nr
Match: XP_022926641.1 (CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita moschata] >KAG6594541.1 CBL-interacting serine/threonine-protein kinase 12, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026520.1 CBL-interacting serine/threonine-protein kinase 12, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 873.2 bits (2255), Expect = 9.8e-250
Identity = 446/474 (94.09%), Postives = 457/474 (96.41%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPD DKKTETT+KDMPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDV---DSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQS SESDSEIIETRR+  +LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420
           GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPKLVMVEVKRKGGDKAEYEQFCNKELKPALLNLK--VEDSAAA--AHIQSDTE 468
           T KLVMVEVKRKGGDKAEYEQFCN+ELKPALLNLK  VEDSAAA  +HI SDTE
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDTE 474

BLAST of Lag0013913 vs. NCBI nr
Match: XP_023518648.1 (CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 872.8 bits (2254), Expect = 1.3e-249
Identity = 445/474 (93.88%), Postives = 457/474 (96.41%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPD DKKTETT+KDMPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCS+E
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDV---DSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQS SESDSEIIETRR+  +LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420
           GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPKLVMVEVKRKGGDKAEYEQFCNKELKPALLNLK--VEDSAAA--AHIQSDTE 468
           T KLVMVEVKRKGGDKAEYEQFCN+ELKPALLNLK  VEDSAAA  +HI SDTE
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDTE 474

BLAST of Lag0013913 vs. ExPASy Swiss-Prot
Match: Q9SN43 (CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=3702 GN=CIPK12 PE=1 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 9.3e-219
Identity = 380/462 (82.25%), Postives = 412/462 (89.18%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MA++   +      R    AL+LGRYE+GKLLGHGTFAKVY ARN+KTNESVAIKVIDKE
Sbjct: 1   MAEKITRETSLPKERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           K+LKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELF KV+KGRL
Sbjct: 61  KVLKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGY+AAKVDIWSCGVILFVLMAGYLPFHD+N+MAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFS EL RLL++LL+TNPE RFT PEIMEN WFKKG+KHIKFY+EDDK+C+V 
Sbjct: 241 GEFRCPRWFSTELTRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVV 300

Query: 301 DD----NDDVDSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFE 360
           DD    +D V+S  D + SES+ E +E RRR G LPRPASLNAFDIISFS GFDLSGLF+
Sbjct: 301 DDDELESDSVESDRDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFD 360

Query: 361 DGGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFE 420
           D GE +RFVS APVSKIISKLEEIAK+VSFTVRKKDCRVSLEGSR+GVKGPLTIAAE+FE
Sbjct: 361 DDGEGSRFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFE 420

Query: 421 LTPKLVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAA 459
           LTP LV+VEVK+KGGDK EYE FCN ELKP L NL  +D  A
Sbjct: 421 LTPSLVVVEVKKKGGDKTEYEDFCNNELKPKLQNLTADDVVA 462

BLAST of Lag0013913 vs. ExPASy Swiss-Prot
Match: Q9FJ55 (CBL-interacting serine/threonine-protein kinase 19 OS=Arabidopsis thaliana OX=3702 GN=CIPK19 PE=1 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 9.3e-203
Identity = 351/453 (77.48%), Postives = 405/453 (89.40%), Query Frame = 0

Query: 10  KTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIA 69
           K +  + +  AL+LG+YE+G+LLGHGTFAKVY ARN ++ ESVAIKVIDKEK+LK GLIA
Sbjct: 12  KKKQDQSNHQALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIA 71

Query: 70  HIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYF 129
           HIKREISILRRVRHPNIVQLFEVMATK+KIYFVMEYV+GGELF KV+KGRLKEE+ARKYF
Sbjct: 72  HIKREISILRRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYF 131

Query: 130 QQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 189
           QQLISAV FCH RGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP
Sbjct: 132 QQLISAVSFCHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 191

Query: 190 AYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWF 249
           AYVAPEVLARKGY+ AKVDIWSCGVILFVLMAG+LPFHD+N+MAMYKKIY+G+FRCPRWF
Sbjct: 192 AYVAPEVLARKGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWF 251

Query: 250 SPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDD-KVCSVEDDN--DDV 309
             E+ RLL R+L+T PE RFT+P+IME  WFKKG+KHIKFY+EDD ++C+V DD+  + +
Sbjct: 252 PVEINRLLIRMLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVADDDEIESI 311

Query: 310 DSLSDQSLSESDSEIIET---RRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEAR 369
           +S+S +S + S+ E  E+   RRR GS+PRPASLNAFD+ISFSPGFDLSGLFED GE +R
Sbjct: 312 ESVSGRSSTVSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFEDDGEGSR 371

Query: 370 FVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREG-VKGPLTIAAEVFELTPKLV 429
           FVS APV +IISKLEEIA++VSFTVRKKDC+VSLEGSREG +KGPL+IAAE+FELTP LV
Sbjct: 372 FVSGAPVGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPALV 431

Query: 430 MVEVKRKGGDKAEYEQFCNKELKPALLNLKVED 456
           +VEVK+KGGDK EY++FCNKELKP L NL  E+
Sbjct: 432 VVEVKKKGGDKMEYDEFCNKELKPKLQNLSSEN 464

BLAST of Lag0013913 vs. ExPASy Swiss-Prot
Match: Q9LP51 (CBL-interacting serine/threonine-protein kinase 18 OS=Arabidopsis thaliana OX=3702 GN=CIPK18 PE=1 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 1.4e-195
Identity = 339/452 (75.00%), Postives = 391/452 (86.50%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           +L+G+YE+GKLLGHGTFAKVY A+NIK+ + VAIKVIDKEKI+K GL+AHIKREISILRR
Sbjct: 69  ILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKIMKSGLVAHIKREISILRR 128

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVGFCH 140
           VRHP IV LFEVMATK+KIYFVMEYV GGELF  V+KGRL EE AR+YFQQLIS+V FCH
Sbjct: 129 VRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPEETARRYFQQLISSVSFCH 188

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARK 200
            RGVYHRDLKPENLLLD  GNLKVSDFGLSAV++Q+RQDGL HTFCGTPAY+APEVL RK
Sbjct: 189 GRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLCHTFCGTPAYIAPEVLTRK 248

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRL 260
           GY+AAK D+WSCGVILFVLMAG++PF+D+NIM MYKKIYKGEFRCPRWFS +L+RLLTRL
Sbjct: 249 GYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGEFRCPRWFSSDLVRLLTRL 308

Query: 261 LDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLSDQS--LSESD 320
           LDTNP+TR TIPEIM+NRWFKKG+KH+KFYIEDDK+C  ++D ++  S S +S  +SESD
Sbjct: 309 LDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDEDEEEEASSSGRSSTVSESD 368

Query: 321 SEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLF-EDGGEEARFVSSAPVSKIISKL 380
           +E    R   GS+PRP+SLNAFDIISFS GFDLSGLF E+GGE  RFVS APVSKIISKL
Sbjct: 369 AEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEEEGGEGTRFVSGAPVSKIISKL 428

Query: 381 EEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPKLVMVEVKRKGGDKAEYE 440
           EEIAK+VSFTVRKK+  + LEG REG KGPLTIAAE+FELTP LV+VEVK+KGGD+ EYE
Sbjct: 429 EEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFELTPSLVVVEVKKKGGDREEYE 488

Query: 441 QFCNKELKPALLNLKVEDSAA--AAHIQSDTE 468
           +FCNKEL+P L  L  E+     A ++ SDTE
Sbjct: 489 EFCNKELRPELEKLIHEEVVVEEALYLPSDTE 520

BLAST of Lag0013913 vs. ExPASy Swiss-Prot
Match: Q68Y49 (CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK19 PE=1 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 1.1e-182
Identity = 319/478 (66.74%), Postives = 386/478 (80.75%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           LLLGRYE+G+LLGHGTFAKVY AR+  + E VAIKV+DKEK ++ GL+ HIKREI+ILRR
Sbjct: 32  LLLGRYELGRLLGHGTFAKVYQARSADSGEPVAIKVLDKEKAMRHGLVPHIKREIAILRR 91

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVGFCH 140
           VRHPNIV+LFEVMATK+KIYFVME VRGGELF +V+KGRLKE+ AR+YFQQL+SAVGFCH
Sbjct: 92  VRHPNIVRLFEVMATKSKIYFVMELVRGGELFGRVAKGRLKEDTARRYFQQLVSAVGFCH 151

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARK 200
           ARGV+HRDLKPENLL+DE+G+LKVSDFGLSAV+DQ   DGL HTFCGTP+YVAPEVLAR+
Sbjct: 152 ARGVFHRDLKPENLLVDEHGDLKVSDFGLSAVADQFHPDGLLHTFCGTPSYVAPEVLARR 211

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRL 260
           GY+ AK DIWSCG+ILFVLMAGYLPFHDQN+MAMY+KIY+GEFRCPRWFS +L  LL R+
Sbjct: 212 GYDGAKADIWSCGIILFVLMAGYLPFHDQNLMAMYRKIYRGEFRCPRWFSKDLSSLLNRI 271

Query: 261 LDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSL----------- 320
           LDTNPETR T+ E+ME+RWF+KG++ ++FY+EDD+V S+ D ++D+  L           
Sbjct: 272 LDTNPETRITVKEVMESRWFQKGFRPVRFYVEDDQVHSLADGDNDMPELEPSEPPPPPPF 331

Query: 321 ----------SDQSLSESDSEII---------ETRRRP-GSLPRPASLNAFDIISFSPGF 380
                      ++S  ESDS +          E R+RP GSL RPASLNAFDIISFS GF
Sbjct: 332 PPPPPQQDDDGEESGWESDSSVASCPATLSSEERRQRPLGSLTRPASLNAFDIISFSKGF 391

Query: 381 DLSGLFEDGGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLT 440
           DLSGLFE+ G E RF+S+ P+  II+KLEEIAK+ SF VR+KD RVS+EG+REG+KGPLT
Sbjct: 392 DLSGLFEERGSEVRFISAEPMQTIITKLEEIAKVKSFFVRRKDWRVSIEGTREGLKGPLT 451

Query: 441 IAAEVFELTPKLVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAAAAHIQSDTE 468
           I AE+FELTP LV+VEVK+K GDK EY+ FCN+ELKP + +L V    +  +I SDTE
Sbjct: 452 IGAEIFELTPSLVVVEVKKKAGDKEEYDDFCNRELKPGMQHL-VHHMGSVPNIPSDTE 508

BLAST of Lag0013913 vs. ExPASy Swiss-Prot
Match: O22971 (CBL-interacting serine/threonine-protein kinase 13 OS=Arabidopsis thaliana OX=3702 GN=CIPK13 PE=1 SV=2)

HSP 1 Score: 631.7 bits (1628), Expect = 6.5e-180
Identity = 314/442 (71.04%), Postives = 367/442 (83.03%), Query Frame = 0

Query: 15  RKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKRE 74
           R    ++L+ +YEIGKLLGHG+FAKVY ARNI + E VAIKVIDKEKI+K GL  HIKRE
Sbjct: 46  RTPQGSILMDKYEIGKLLGHGSFAKVYLARNIHSGEDVAIKVIDKEKIVKSGLAGHIKRE 105

Query: 75  ISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLIS 134
           ISILRRVRHP IV L EVMATK KIY VMEYVRGGEL+  V++GRL+E  AR+YFQQLIS
Sbjct: 106 ISILRRVRHPYIVHLLEVMATKTKIYIVMEYVRGGELYNTVARGRLREGTARRYFQQLIS 165

Query: 135 AVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAP 194
           +V FCH+RGVYHRDLK ENLLLD+ GN+KVSDFGLS VS+Q++Q+G+  TFCGTPAY+AP
Sbjct: 166 SVAFCHSRGVYHRDLKLENLLLDDKGNVKVSDFGLSVVSEQLKQEGICQTFCGTPAYLAP 225

Query: 195 EVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELI 254
           EVL RKGYE AK DIWSCGVILFVLMAGYLPF D+NI+ MY KIYKG+F+CP+WFSPEL 
Sbjct: 226 EVLTRKGYEGAKADIWSCGVILFVLMAGYLPFDDKNILVMYTKIYKGQFKCPKWFSPELA 285

Query: 255 RLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLS---- 314
           RL+TR+LDTNP+TR TIPEIM++RWFKKG+KH+KFYIE+DK+C  +DDNDD DS S    
Sbjct: 286 RLVTRMLDTNPDTRITIPEIMKHRWFKKGFKHVKFYIENDKLCREDDDNDDDDSSSLSSG 345

Query: 315 -DQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEARFVSSAP 374
              + SE D+E     +R  S+PRPASLNAFDI+SFS   DLSGLFE+GG+ ARFVS+AP
Sbjct: 346 RSSTASEGDAEF--DIKRVDSMPRPASLNAFDILSFS---DLSGLFEEGGQGARFVSAAP 405

Query: 375 VSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPKLVMVEVKRK 434
           ++KIISKLEEIAK V F VRKKD  V LEG REG KGPLTI  E+FELTP LV+VEVK+K
Sbjct: 406 MTKIISKLEEIAKEVKFMVRKKDWSVRLEGCREGAKGPLTIRVEIFELTPSLVVVEVKKK 465

Query: 435 GGDKAEYEQFCNKELKPALLNL 452
           GG+  EYE+FCNKEL+P L  L
Sbjct: 466 GGNIEEYEEFCNKELRPQLEKL 482

BLAST of Lag0013913 vs. ExPASy TrEMBL
Match: A0A5A7UB69 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008270 PE=3 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 8.6e-260
Identity = 456/467 (97.64%), Postives = 458/467 (98.07%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDNDDVDSLSDQS SESDSEIIETRR+  SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420
           EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAAAAHIQSDTE 468
           LVMVEVKRKGGDKAEYEQFCN ELKPALLNLKVEDS   +HI SDTE
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Lag0013913 vs. ExPASy TrEMBL
Match: A0A1S3AZF1 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103484445 PE=3 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 8.6e-260
Identity = 456/467 (97.64%), Postives = 458/467 (98.07%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDNDDVDSLSDQS SESDSEIIETRR+  SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420
           EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAAAAHIQSDTE 468
           LVMVEVKRKGGDKAEYEQFCN ELKPALLNLKVEDS   +HI SDTE
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Lag0013913 vs. ExPASy TrEMBL
Match: A0A0A0KLP8 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6G519640 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 4.7e-258
Identity = 452/467 (96.79%), Postives = 456/467 (97.64%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTN+SVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNDSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCS+E
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDVDSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DD DDVDSLSDQS SESDSEIIETRR+  SLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDTDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420
           EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAAAAHIQSDTE 468
           LVMVEVKRKGGDK EYEQFCN ELKPALLNLKVEDS   +HI SDTE
Sbjct: 421 LVMVEVKRKGGDKEEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467

BLAST of Lag0013913 vs. ExPASy TrEMBL
Match: A0A6J1EFG7 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111433713 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 4.7e-250
Identity = 446/474 (94.09%), Postives = 457/474 (96.41%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPD DKKTETT+KDMPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDV---DSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQS SESDSEIIETRR+  +LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420
           GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPKLVMVEVKRKGGDKAEYEQFCNKELKPALLNLK--VEDSAAA--AHIQSDTE 468
           T KLVMVEVKRKGGDKAEYEQFCN+ELKPALLNLK  VEDSAAA  +HI SDTE
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDTE 474

BLAST of Lag0013913 vs. ExPASy TrEMBL
Match: A0A6J1KSW8 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111496943 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.1e-249
Identity = 444/474 (93.67%), Postives = 456/474 (96.20%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPD DKKTETT+KDMPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEI ENRWFKKGYKHIKFYIEDDKVCS+E
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEITENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDV---DSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQS SESDSEIIETRR+  +LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420
           GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPKLVMVEVKRKGGDKAEYEQFCNKELKPALLNLK--VEDSAAA--AHIQSDTE 468
           T KLVMVEVKRKGGDKAEYEQFCN+ELKPALLNLK  VEDSAAA  +HI SDTE
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDTE 474

BLAST of Lag0013913 vs. TAIR 10
Match: AT4G18700.1 (CBL-interacting protein kinase 12 )

HSP 1 Score: 760.8 bits (1963), Expect = 6.6e-220
Identity = 380/462 (82.25%), Postives = 412/462 (89.18%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MA++   +      R    AL+LGRYE+GKLLGHGTFAKVY ARN+KTNESVAIKVIDKE
Sbjct: 1   MAEKITRETSLPKERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           K+LKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELF KV+KGRL
Sbjct: 61  KVLKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRL 120

Query: 121 KEEVARKYFQQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGY+AAKVDIWSCGVILFVLMAGYLPFHD+N+MAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFS EL RLL++LL+TNPE RFT PEIMEN WFKKG+KHIKFY+EDDK+C+V 
Sbjct: 241 GEFRCPRWFSTELTRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVV 300

Query: 301 DD----NDDVDSLSDQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFE 360
           DD    +D V+S  D + SES+ E +E RRR G LPRPASLNAFDIISFS GFDLSGLF+
Sbjct: 301 DDDELESDSVESDRDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFD 360

Query: 361 DGGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFE 420
           D GE +RFVS APVSKIISKLEEIAK+VSFTVRKKDCRVSLEGSR+GVKGPLTIAAE+FE
Sbjct: 361 DDGEGSRFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFE 420

Query: 421 LTPKLVMVEVKRKGGDKAEYEQFCNKELKPALLNLKVEDSAA 459
           LTP LV+VEVK+KGGDK EYE FCN ELKP L NL  +D  A
Sbjct: 421 LTPSLVVVEVKKKGGDKTEYEDFCNNELKPKLQNLTADDVVA 462

BLAST of Lag0013913 vs. TAIR 10
Match: AT5G45810.1 (CBL-interacting protein kinase 19 )

HSP 1 Score: 707.6 bits (1825), Expect = 6.6e-204
Identity = 351/453 (77.48%), Postives = 405/453 (89.40%), Query Frame = 0

Query: 10  KTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIA 69
           K +  + +  AL+LG+YE+G+LLGHGTFAKVY ARN ++ ESVAIKVIDKEK+LK GLIA
Sbjct: 12  KKKQDQSNHQALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIA 71

Query: 70  HIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYF 129
           HIKREISILRRVRHPNIVQLFEVMATK+KIYFVMEYV+GGELF KV+KGRLKEE+ARKYF
Sbjct: 72  HIKREISILRRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYF 131

Query: 130 QQLISAVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 189
           QQLISAV FCH RGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP
Sbjct: 132 QQLISAVSFCHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 191

Query: 190 AYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWF 249
           AYVAPEVLARKGY+ AKVDIWSCGVILFVLMAG+LPFHD+N+MAMYKKIY+G+FRCPRWF
Sbjct: 192 AYVAPEVLARKGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWF 251

Query: 250 SPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDD-KVCSVEDDN--DDV 309
             E+ RLL R+L+T PE RFT+P+IME  WFKKG+KHIKFY+EDD ++C+V DD+  + +
Sbjct: 252 PVEINRLLIRMLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVADDDEIESI 311

Query: 310 DSLSDQSLSESDSEIIET---RRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEAR 369
           +S+S +S + S+ E  E+   RRR GS+PRPASLNAFD+ISFSPGFDLSGLFED GE +R
Sbjct: 312 ESVSGRSSTVSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFEDDGEGSR 371

Query: 370 FVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREG-VKGPLTIAAEVFELTPKLV 429
           FVS APV +IISKLEEIA++VSFTVRKKDC+VSLEGSREG +KGPL+IAAE+FELTP LV
Sbjct: 372 FVSGAPVGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPALV 431

Query: 430 MVEVKRKGGDKAEYEQFCNKELKPALLNLKVED 456
           +VEVK+KGGDK EY++FCNKELKP L NL  E+
Sbjct: 432 VVEVKKKGGDKMEYDEFCNKELKPKLQNLSSEN 464

BLAST of Lag0013913 vs. TAIR 10
Match: AT1G29230.1 (CBL-interacting protein kinase 18 )

HSP 1 Score: 683.7 bits (1763), Expect = 1.0e-196
Identity = 339/452 (75.00%), Postives = 391/452 (86.50%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           +L+G+YE+GKLLGHGTFAKVY A+NIK+ + VAIKVIDKEKI+K GL+AHIKREISILRR
Sbjct: 69  ILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKIMKSGLVAHIKREISILRR 128

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVGFCH 140
           VRHP IV LFEVMATK+KIYFVMEYV GGELF  V+KGRL EE AR+YFQQLIS+V FCH
Sbjct: 129 VRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPEETARRYFQQLISSVSFCH 188

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARK 200
            RGVYHRDLKPENLLLD  GNLKVSDFGLSAV++Q+RQDGL HTFCGTPAY+APEVL RK
Sbjct: 189 GRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLCHTFCGTPAYIAPEVLTRK 248

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRL 260
           GY+AAK D+WSCGVILFVLMAG++PF+D+NIM MYKKIYKGEFRCPRWFS +L+RLLTRL
Sbjct: 249 GYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGEFRCPRWFSSDLVRLLTRL 308

Query: 261 LDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLSDQS--LSESD 320
           LDTNP+TR TIPEIM+NRWFKKG+KH+KFYIEDDK+C  ++D ++  S S +S  +SESD
Sbjct: 309 LDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDEDEEEEASSSGRSSTVSESD 368

Query: 321 SEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLF-EDGGEEARFVSSAPVSKIISKL 380
           +E    R   GS+PRP+SLNAFDIISFS GFDLSGLF E+GGE  RFVS APVSKIISKL
Sbjct: 369 AEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEEEGGEGTRFVSGAPVSKIISKL 428

Query: 381 EEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPKLVMVEVKRKGGDKAEYE 440
           EEIAK+VSFTVRKK+  + LEG REG KGPLTIAAE+FELTP LV+VEVK+KGGD+ EYE
Sbjct: 429 EEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFELTPSLVVVEVKKKGGDREEYE 488

Query: 441 QFCNKELKPALLNLKVEDSAA--AAHIQSDTE 468
           +FCNKEL+P L  L  E+     A ++ SDTE
Sbjct: 489 EFCNKELRPELEKLIHEEVVVEEALYLPSDTE 520

BLAST of Lag0013913 vs. TAIR 10
Match: AT2G34180.1 (CBL-interacting protein kinase 13 )

HSP 1 Score: 631.7 bits (1628), Expect = 4.6e-181
Identity = 314/442 (71.04%), Postives = 367/442 (83.03%), Query Frame = 0

Query: 15  RKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKRE 74
           R    ++L+ +YEIGKLLGHG+FAKVY ARNI + E VAIKVIDKEKI+K GL  HIKRE
Sbjct: 46  RTPQGSILMDKYEIGKLLGHGSFAKVYLARNIHSGEDVAIKVIDKEKIVKSGLAGHIKRE 105

Query: 75  ISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLIS 134
           ISILRRVRHP IV L EVMATK KIY VMEYVRGGEL+  V++GRL+E  AR+YFQQLIS
Sbjct: 106 ISILRRVRHPYIVHLLEVMATKTKIYIVMEYVRGGELYNTVARGRLREGTARRYFQQLIS 165

Query: 135 AVGFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAP 194
           +V FCH+RGVYHRDLK ENLLLD+ GN+KVSDFGLS VS+Q++Q+G+  TFCGTPAY+AP
Sbjct: 166 SVAFCHSRGVYHRDLKLENLLLDDKGNVKVSDFGLSVVSEQLKQEGICQTFCGTPAYLAP 225

Query: 195 EVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELI 254
           EVL RKGYE AK DIWSCGVILFVLMAGYLPF D+NI+ MY KIYKG+F+CP+WFSPEL 
Sbjct: 226 EVLTRKGYEGAKADIWSCGVILFVLMAGYLPFDDKNILVMYTKIYKGQFKCPKWFSPELA 285

Query: 255 RLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLS---- 314
           RL+TR+LDTNP+TR TIPEIM++RWFKKG+KH+KFYIE+DK+C  +DDNDD DS S    
Sbjct: 286 RLVTRMLDTNPDTRITIPEIMKHRWFKKGFKHVKFYIENDKLCREDDDNDDDDSSSLSSG 345

Query: 315 -DQSLSESDSEIIETRRRPGSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEARFVSSAP 374
              + SE D+E     +R  S+PRPASLNAFDI+SFS   DLSGLFE+GG+ ARFVS+AP
Sbjct: 346 RSSTASEGDAEF--DIKRVDSMPRPASLNAFDILSFS---DLSGLFEEGGQGARFVSAAP 405

Query: 375 VSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPKLVMVEVKRK 434
           ++KIISKLEEIAK V F VRKKD  V LEG REG KGPLTI  E+FELTP LV+VEVK+K
Sbjct: 406 MTKIISKLEEIAKEVKFMVRKKDWSVRLEGCREGAKGPLTIRVEIFELTPSLVVVEVKKK 465

Query: 435 GGDKAEYEQFCNKELKPALLNL 452
           GG+  EYE+FCNKEL+P L  L
Sbjct: 466 GGNIEEYEEFCNKELRPQLEKL 482

BLAST of Lag0013913 vs. TAIR 10
Match: AT5G45820.1 (CBL-interacting protein kinase 20 )

HSP 1 Score: 498.4 bits (1282), Expect = 6.1e-141
Identity = 252/435 (57.93%), Postives = 321/435 (73.79%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           +L+ +YE+G+LLG GTFAKVYHARNIKT ESVAIKVIDK+K+ K GLI  IKREIS++R 
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVGFCH 140
           VRHP++V L EVMA+K KIYF MEYV+GGELF KVSKG+LKE +ARKYFQQLI A+ +CH
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVSKGKLKENIARKYFQQLIGAIDYCH 126

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARK 200
           +RGVYHRDLKPENLLLDENG+LK+SDFGLSA+ +  +QDGL HT CGTPAYVAPEV+ +K
Sbjct: 127 SRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGKK 186

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRL 260
           GY+ AK D+WSCGV+L+VL+AG+LPFH+QN++ MY+KI KGEF+CP WF PE+ +LL+R+
Sbjct: 187 GYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSRI 246

Query: 261 LDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSL-SDQSLSESDS 320
           LD NP +R  I +IMEN WF+KG+K I+             ++  +DSL SD   + S  
Sbjct: 247 LDPNPNSRIKIEKIMENSWFQKGFKKIE--------TPKSPESHQIDSLISDVHAAFS-- 306

Query: 321 EIIETRRRPGSLPRPASLNAFDII-SFSPGFDLSGLFE-DGGEEARFVSSAPVSKIISKL 380
                        +P S NAFD+I S S GFDLSGLFE +   E++F +     +I+SK 
Sbjct: 307 ------------VKPMSYNAFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKF 366

Query: 381 EEIA-KLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPKLVMVEVKRKGGDKAEY 440
           EEIA     F + K D  V +E  REG KG L I  E+FE+T    MVE K+ GGD  EY
Sbjct: 367 EEIATSSERFNLTKSDVGVKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEY 419

Query: 441 EQFCNKELKPALLNL 452
           +QFC++EL+P+L ++
Sbjct: 427 KQFCDRELRPSLKDI 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008439731.11.8e-25997.64PREDICTED: CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo... [more]
XP_038882513.13.0e-25997.43CBL-interacting serine/threonine-protein kinase 12-like [Benincasa hispida][more]
XP_011658253.19.8e-25896.79CBL-interacting serine/threonine-protein kinase 12 [Cucumis sativus] >KGN49312.1... [more]
XP_022926641.19.8e-25094.09CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita moschata] >KA... [more]
XP_023518648.11.3e-24993.88CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
Q9SN439.3e-21982.25CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=37... [more]
Q9FJ559.3e-20377.48CBL-interacting serine/threonine-protein kinase 19 OS=Arabidopsis thaliana OX=37... [more]
Q9LP511.4e-19575.00CBL-interacting serine/threonine-protein kinase 18 OS=Arabidopsis thaliana OX=37... [more]
Q68Y491.1e-18266.74CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
O229716.5e-18071.04CBL-interacting serine/threonine-protein kinase 13 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A5A7UB698.6e-26097.64Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3AZF18.6e-26097.64Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0KLP84.7e-25896.79Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6... [more]
A0A6J1EFG74.7e-25094.09Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KSW83.1e-24993.67Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G18700.16.6e-22082.25CBL-interacting protein kinase 12 [more]
AT5G45810.16.6e-20477.48CBL-interacting protein kinase 19 [more]
AT1G29230.11.0e-19675.00CBL-interacting protein kinase 18 [more]
AT2G34180.14.6e-18171.04CBL-interacting protein kinase 13 [more]
AT5G45820.16.1e-14157.93CBL-interacting protein kinase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 26..280
e-value: 4.5E-109
score: 378.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 26..280
e-value: 1.5E-75
score: 254.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 26..280
score: 52.314892
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 17..328
e-value: 6.6E-106
score: 356.3
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 332..452
e-value: 2.9E-40
score: 139.2
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 27..294
e-value: 3.1E-12
score: 43.9
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 26..239
e-value: 1.7E-36
score: 123.0
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 30..232
e-value: 2.9E-14
score: 50.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..329
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 19..456
NoneNo IPR availablePANTHERPTHR43895:SF96NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 19..456
NoneNo IPR availableCDDcd14663STKc_SnRK3coord: 25..279
e-value: 3.63683E-171
score: 479.208
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 338..448
e-value: 4.022E-50
score: 164.668
IPR004041NAF domainPFAMPF03822NAFcoord: 334..390
e-value: 4.3E-24
score: 84.4
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 144..156
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 32..55
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 332..356
score: 14.624336
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 22..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013913.1Lag0013913.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity