Lag0013897 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0013897
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	50S ribosomal protein L34
Location	chr1: 53569736 .. 53570709 (+)
RNA-Seq Expression	Lag0013897
Synteny	Lag0013897

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCGATATCAGTATTATCGTCCCCATGGGTTTCGACCGGAGGCATGGCCGCGCGCGTTCCTTCAGCTTCTCTTGTAATCTCCACTGGCTCTAGAAGTTGCTCCGTATCTCTTAATACCGCAAACAACACTTTCGCTCGTTCTGGACTGCTTCATTGTTCGTTTCTTCCTTCTTCCTCTATTTCGTGTGCTTCATCTTTCTCAGGTGAGGAATTTTTATACTCTCAAATTTCTGTATTAGTTCAAGTAGTTTAATGATTTCGCATTATTGCTTGCTCATTTGCCAATATAATGGAGCCTATAGCAAGTAGTTCACTGAAGAATTGATTTGCTTTCGTGTGCGTTTATCACTTTCTAGGCCCTTTCATTTCGAATTGTATACATTCAAGGTCGTTTGATTTGATAATTCCACAGTTGTTCGATGGCATAATTGCCGGATGGTCGATTTCTGCTTGCATTTGATTTGTTATGAATCCTCTCGTATTTTCCTTTTCGTGCTTTCCAGTAACCGAATTGTAAGTGAATGAAAGTTGGTGCAGGTGACTGCATGTCTCCATTTTTTATATCTAAGAATCCATTGTGGTTCCCTTGTTAAGGAGAAAATAAAAGAATATTCACCGCTGCCTGCAGCATTGCGTAGCTTCTCTTGCGATATTTAGTTCCGTTGATCAAGATTTGACATTAACTTTATGGGAAAGGAGTACTCATTTGCAGGTTTATCTTTGGGTTTGGATTGGAGCTCTAAAATTGAAGTTGGACAAGGGAAGGGCCGGAGCTTAGTGGTTAGGGCTGGCAAGGCTGCACTTTGTCAGACTAAAAGAAACAGGTCGCGTAAATCCTTAGCTCGGACTCATGGTTTTCGCCGACGAATGCGAACTACCAATGGAAGAGCTGTTTTAAAGCGCAGACGTGCCAAGGGTAGGAAGGTCCTCTGCACAAAGTCCAATCCAAGCAGCGGAAAGGGTGCTTAA

mRNA sequence

ATGGCAGCGATATCAGTATTATCGTCCCCATGGGTTTCGACCGGAGGCATGGCCGCGCGCGTTCCTTCAGCTTCTCTTGTAATCTCCACTGGCTCTAGAAGTTGCTCCGTATCTCTTAATACCGCAAACAACACTTTCGCTCGTTCTGGACTGCTTCATTGTTCGTTTCTTCCTTCTTCCTCTATTTCGTGTGCTTCATCTTTCTCAGGTTTATCTTTGGGTTTGGATTGGAGCTCTAAAATTGAAGTTGGACAAGGGAAGGGCCGGAGCTTAGTGGTTAGGGCTGGCAAGGCTGCACTTTGTCAGACTAAAAGAAACAGGTCGCGTAAATCCTTAGCTCGGACTCATGGTTTTCGCCGACGAATGCGAACTACCAATGGAAGAGCTGTTTTAAAGCGCAGACGTGCCAAGGGTAGGAAGGTCCTCTGCACAAAGTCCAATCCAAGCAGCGGAAAGGGTGCTTAA

Coding sequence (CDS)

ATGGCAGCGATATCAGTATTATCGTCCCCATGGGTTTCGACCGGAGGCATGGCCGCGCGCGTTCCTTCAGCTTCTCTTGTAATCTCCACTGGCTCTAGAAGTTGCTCCGTATCTCTTAATACCGCAAACAACACTTTCGCTCGTTCTGGACTGCTTCATTGTTCGTTTCTTCCTTCTTCCTCTATTTCGTGTGCTTCATCTTTCTCAGGTTTATCTTTGGGTTTGGATTGGAGCTCTAAAATTGAAGTTGGACAAGGGAAGGGCCGGAGCTTAGTGGTTAGGGCTGGCAAGGCTGCACTTTGTCAGACTAAAAGAAACAGGTCGCGTAAATCCTTAGCTCGGACTCATGGTTTTCGCCGACGAATGCGAACTACCAATGGAAGAGCTGTTTTAAAGCGCAGACGTGCCAAGGGTAGGAAGGTCCTCTGCACAAAGTCCAATCCAAGCAGCGGAAAGGGTGCTTAA

Protein sequence

MAAISVLSSPWVSTGGMAARVPSASLVISTGSRSCSVSLNTANNTFARSGLLHCSFLPSSSISCASSFSGLSLGLDWSSKIEVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFRRRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKGA

Homology

[Close]

BLAST of Lag0013897 vs. NCBI nr
Match: XP_008439748.1 (PREDICTED: 50S ribosomal protein L34, chloroplastic [Cucumis melo] >TYK13199.1 50S ribosomal protein L34 [Cucumis melo var. makuwa])

HSP 1 Score: 256.9 bits (655), Expect = 1.1e-64
Identity = 144/155 (92.90%), Postives = 148/155 (95.48%), Query Frame = 0


Query: 1   MAAISVLSSPWVSTGGMAARVPSASLVISTGSR-SCSVSLNTANNTFARSGLLHCSFLPS 60
           MAAISVLSSPWVS G +A RVPSASLVISTGSR SCSVSLNTANNT ARSGLLHCSFLPS
Sbjct: 1   MAAISVLSSPWVSNGAIAVRVPSASLVISTGSRGSCSVSLNTANNTSARSGLLHCSFLPS 60

Query: 61  SSISCASSFSGLSLGLDWSSKIEVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR 120
           SS+SC+SSFSGLSLGLDWSSK+ VGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR
Sbjct: 61  SSLSCSSSFSGLSLGLDWSSKVGVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR 120

Query: 121 RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKGA 155
           RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGK A
Sbjct: 121 RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKRA 155

[Close]

BLAST of Lag0013897 vs. NCBI nr
Match: XP_038883793.1 (50S ribosomal protein L34, chloroplastic [Benincasa hispida])

HSP 1 Score: 251.5 bits (641), Expect = 4.6e-63
Identity = 143/155 (92.26%), Postives = 145/155 (93.55%), Query Frame = 0


Query: 1   MAAISVLSSPWVSTGGMAARVPSASLVISTGSRS-CSVSLNTANNTFARSGLLHCSFLPS 60
           MAAISVLSSPWVSTG  A RVPSASLVISTG R  CSVSLNTANNT ARSGLLHCSFL S
Sbjct: 1   MAAISVLSSPWVSTGATAVRVPSASLVISTGFRGCCSVSLNTANNTSARSGLLHCSFLSS 60

Query: 61  SSISCASSFSGLSLGLDWSSKIEVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR 120
           SS+SC+SSFSGLSLGLDWSSKI VGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR
Sbjct: 61  SSLSCSSSFSGLSLGLDWSSKIGVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR 120

Query: 121 RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKGA 155
           RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGK A
Sbjct: 121 RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKRA 155

[Close]

BLAST of Lag0013897 vs. NCBI nr
Match: XP_004134668.1 (50S ribosomal protein L34, chloroplastic [Cucumis sativus] >KGN49298.1 hypothetical protein Csa_003653 [Cucumis sativus])

HSP 1 Score: 249.6 bits (636), Expect = 1.8e-62
Identity = 139/155 (89.68%), Postives = 144/155 (92.90%), Query Frame = 0


Query: 1   MAAISVLSSPWVSTGGMAARVPSASLVISTGSRS-CSVSLNTANNTFARSGLLHCSFLPS 60
           MAAISVLS+PWVSTG +A RVPSASLV STGSR  CSVSLNT NN  ARSGLLHCSFLPS
Sbjct: 1   MAAISVLSTPWVSTGAIAVRVPSASLVFSTGSRGYCSVSLNTVNNNSARSGLLHCSFLPS 60

Query: 61  SSISCASSFSGLSLGLDWSSKIEVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR 120
           SS+SC+SSFSGLSLGLDWSSK+ VGQGKGR LVVRAGKAALCQTKRNRSRKSLARTHGFR
Sbjct: 61  SSLSCSSSFSGLSLGLDWSSKVGVGQGKGRGLVVRAGKAALCQTKRNRSRKSLARTHGFR 120

Query: 121 RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKGA 155
           RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGK A
Sbjct: 121 RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKRA 155

[Close]

BLAST of Lag0013897 vs. NCBI nr
Match: XP_022142342.1 (50S ribosomal protein L34, chloroplastic [Momordica charantia])

HSP 1 Score: 245.0 bits (624), Expect = 4.3e-61
Identity = 138/155 (89.03%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 1   MAAISVLSSPWVSTGGMAARVPSASLVISTGSRS-CSVSLNTANNTFARSGLLHCSFLPS 60
           MAAISVLS+PWV +G +A RVPSASLVISTGSRS CSVSLNTANNT ARSGLL CSFLPS
Sbjct: 1   MAAISVLSTPWVLSGAVAVRVPSASLVISTGSRSCCSVSLNTANNTSARSGLLRCSFLPS 60

Query: 61  SSISCASSFSGLSLGLDWSSKIEVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR 120
           SS+SC+SSFSG SLGLDW+S+I VGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR
Sbjct: 61  SSLSCSSSFSGFSLGLDWNSRIWVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFR 120

Query: 121 RRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKGA 155
           RRMRTT+GRAVLKRRRAKGRKVLCTKS PSSGKGA
Sbjct: 121 RRMRTTSGRAVLKRRRAKGRKVLCTKSYPSSGKGA 155

[Close]

BLAST of Lag0013897 vs. NCBI nr
Match: XP_022926470.1 (50S ribosomal protein L34, chloroplastic-like [Cucurbita moschata] >XP_023517338.1 50S ribosomal protein L34, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6594552.1 50S ribosomal protein L34, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026528.1 50S ribosomal protein L34, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 229.6 bits (584), Expect = 1.9e-56
Identity = 134/154 (87.01%), Postives = 137/154 (88.96%), Query Frame = 0


Query: 1   MAAISVLSSPWVSTGGMAARVPSASLVISTGSRSCSVSLNTANNTFARSGLLHCSFLPSS 60
           MAAISV+SS  VST     RVPSASLV STGSRS S+SLNTANN  AR GLLHCSFLPSS
Sbjct: 1   MAAISVISSLCVST----VRVPSASLVFSTGSRSSSISLNTANNASARPGLLHCSFLPSS 60

Query: 61  SISCASSFSGLSLGLDWSSKIEVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTHGFRR 120
           S+SC SSFSGLSLGLDWS KI VGQGK RSLVVRAGKAALCQTKRNRSRKSLARTHGFRR
Sbjct: 61  SLSCKSSFSGLSLGLDWSPKIGVGQGKRRSLVVRAGKAALCQTKRNRSRKSLARTHGFRR 120

Query: 121 RMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKGA 155
           RMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGK A
Sbjct: 121 RMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKRA 150

[Close]

BLAST of Lag0013897 vs. ExPASy Swiss-Prot
Match: P82244 (50S ribosomal protein L34, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL34 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 3.6e-34
Identity = 102/158 (64.56%), Postives = 117/158 (74.05%), Query Frame = 0


Query: 1   MAAISVLSSPWVSTGGMAA---RVPSASLVISTGSRSCSVSLNTANNTFARSGLLHCSFL 60
           MA +S+L     STG  AA   R PSASL   TGSR+ +  + + N   ARSG LHCSFL
Sbjct: 1   MATLSLL-----STGVGAAITNRTPSASLTFITGSRTTNKRV-SFNGGSARSGSLHCSFL 60

Query: 61  -PSSSISCASSFSGLSLGLDWSSKIEVGQGKGRSLVVRAGKAALCQTKRNRSRKSLARTH 120
            PSSS+S  S+FSGLSLGLD +S   V   + R  VVRAGKAALC TKR+RSRKSLARTH
Sbjct: 61  APSSSLS--SNFSGLSLGLDLTSNTGVSTDRCRRFVVRAGKAALCLTKRSRSRKSLARTH 120

Query: 121 GFRRRMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKGA 155
           GFR RM TT+GRA+LKRRRAKGRK+LCTK+NPSSGK A
Sbjct: 121 GFRLRMSTTSGRALLKRRRAKGRKILCTKTNPSSGKRA 150

[Close]

BLAST of Lag0013897 vs. ExPASy Swiss-Prot
Match: Q9LP37 (50S ribosomal protein L34, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL34 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 7.5e-32
Identity = 98/154 (63.64%), Postives = 111/154 (72.08%), Query Frame = 0


Query: 2   AAISVLSSPWVSTGGMAARVPSASLVISTGSRSCSVSLNTANNTFARSGLLHCSFLPSSS 61
           +A S L +P  S G +   VPSASL + TG R  S SL ++    A S LLHCSFL SS 
Sbjct: 11  SASSRLWNPAASNGKIC--VPSASLSLRTGCRRSSSSLTSS----ASSQLLHCSFL-SSP 70

Query: 62  ISCASSFSGLSLGLDWSSKIEVGQG-KGRSLVVRAGKAALCQTKRNRSRKSLARTHGFRR 121
           +S AS FSGLS+  D SS+     G + R LVVRAGKAALCQTKR+RSRKSLARTHGFRR
Sbjct: 71  VSLASPFSGLSIAFDLSSQTSGLNGQRRRGLVVRAGKAALCQTKRSRSRKSLARTHGFRR 130

Query: 122 RMRTTNGRAVLKRRRAKGRKVLCTKSNPSSGKGA 155
           RMRTT+GRA +KRRRAKGR  LC KSNPSSGK A
Sbjct: 131 RMRTTSGRATIKRRRAKGRWNLCPKSNPSSGKRA 157

[Close]

BLAST of Lag0013897 vs. ExPASy Swiss-Prot
Match: Q04CX6 (50S ribosomal protein L34 OS=Oenococcus oeni (strain ATCC BAA-331 / PSU-1) OX=203123 GN=rpmH PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.6e-04
Identity = 24/34 (70.59%), Postives = 27/34 (79.41%), Query Frame = 0


Query: 109 RKSLARTHGFRRRMRTTNGRAVLKRRRAKGRKVL 143
           ++ L R HGFR+RM T NGR VL RRRAKGRKVL
Sbjct: 9   KRHLQRVHGFRKRMSTANGRKVLARRRAKGRKVL 42

[Close]

BLAST of Lag0013897 vs. ExPASy Swiss-Prot
Match: Q48BE9 (50S ribosomal protein L34 OS=Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) OX=264730 GN=rpmH PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.6e-04
Identity = 25/30 (83.33%), Postives = 25/30 (83.33%), Query Frame = 0


Query: 113 ARTHGFRRRMRTTNGRAVLKRRRAKGRKVL 143
           ARTHGFR RM T NGRAVL RRRAKGRK L
Sbjct: 13  ARTHGFRARMATKNGRAVLSRRRAKGRKRL 42

[Close]

BLAST of Lag0013897 vs. ExPASy Swiss-Prot
Match: Q1I2H1 (50S ribosomal protein L34 OS=Pseudomonas entomophila (strain L48) OX=384676 GN=rpmH PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.6e-04
Identity = 25/30 (83.33%), Postives = 25/30 (83.33%), Query Frame = 0


Query: 113 ARTHGFRRRMRTTNGRAVLKRRRAKGRKVL 143
           ARTHGFR RM T NGRAVL RRRAKGRK L
Sbjct: 13  ARTHGFRARMATKNGRAVLSRRRAKGRKRL 42

The following BLAST results are available for this feature:

BLAST of Lag0013897 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008439748.1	1.1e-64	92.90	PREDICTED: 50S ribosomal protein L34, chloroplastic [Cucumis melo] >TYK13199.1 5...	[more]
XP_038883793.1	4.6e-63	92.26	50S ribosomal protein L34, chloroplastic [Benincasa hispida]	[more]
XP_004134668.1	1.8e-62	89.68	50S ribosomal protein L34, chloroplastic [Cucumis sativus] >KGN49298.1 hypotheti...	[more]
XP_022142342.1	4.3e-61	89.03	50S ribosomal protein L34, chloroplastic [Momordica charantia]	[more]
XP_022926470.1	1.9e-56	87.01	50S ribosomal protein L34, chloroplastic-like [Cucurbita moschata] >XP_023517338...	[more]

BLAST of Lag0013897 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P82244	3.6e-34	64.56	50S ribosomal protein L34, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL34 P...	[more]
Q9LP37	7.5e-32	63.64	50S ribosomal protein L34, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL3...	[more]
Q04CX6	8.6e-04	70.59	50S ribosomal protein L34 OS=Oenococcus oeni (strain ATCC BAA-331 / PSU-1) OX=20...	[more]
Q48BE9	8.6e-04	83.33	50S ribosomal protein L34 OS=Pseudomonas savastanoi pv. phaseolicola (strain 144...	[more]
Q1I2H1	8.6e-04	83.33	50S ribosomal protein L34 OS=Pseudomonas entomophila (strain L48) OX=384676 GN=r...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.287.3980		coord: 96..154 e-value: 5.5E-26 score: 91.9
None	No IPR available	PANTHER	PTHR14503:SF9	BNAC06G17900D PROTEIN	coord: 13..153
IPR000271	Ribosomal protein L34	PFAM	PF00468	Ribosomal_L34	coord: 108..143 e-value: 2.0E-12 score: 46.8
IPR000271	Ribosomal protein L34	TIGRFAM	TIGR01030	TIGR01030	coord: 109..142 e-value: 3.8E-11 score: 41.0
IPR000271	Ribosomal protein L34	PANTHER	PTHR14503	MITOCHONDRIAL RIBOSOMAL PROTEIN 34 FAMILY MEMBER	coord: 13..153
IPR000271	Ribosomal protein L34	HAMAP	MF_00391	Ribosomal_L34	coord: 96..144 score: 12.617811

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0013897.1	Lag0013897.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005762	mitochondrial large ribosomal subunit
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003735	structural constituent of ribosome