Lag0013888 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0013888 vs. NCBI nr
Match: XP_038882473.1 (GDSL esterase/lipase At5g45910 isoform X3 [Benincasa hispida])

HSP 1 Score: 265.4 bits (677), Expect = 3.8e-67
Identity = 130/155 (83.87%), Postives = 141/155 (90.97%), Query Frame = 0

Query: 1   MNSVSAFLCLCLISCSSALSKPYNAIFNFGDSLSDTGNFLRSGSLAFPVIGNLPYGQTFF 60
           MNS+ +FL L L  CSSALS PYNAIFNFGDSLSDTGNFLRSG+L+FPVIG LPYG+TFF
Sbjct: 1   MNSLLSFLFLSL--CSSALSTPYNAIFNFGDSLSDTGNFLRSGALSFPVIGKLPYGETFF 60

Query: 61  KHATGRCSDGRLVIDFIAEAFGLRYLPPYLGLKQGQNFSGGVNFAVAGATALDAEFFYKR 120
           +HATGRCSDGRLVIDFIAEAFGL +LPPYLGLKQGQNFS GVNFAVAGATALDA+FFYKR
Sbjct: 61  RHATGRCSDGRLVIDFIAEAFGLPHLPPYLGLKQGQNFSRGVNFAVAGATALDADFFYKR 120

Query: 121 KTGTILWTNDSLSVQLGWFRKLKSSLCTSTQGVDR 156
           K+G ILWTN SL+VQLGWFR LKSSLCTSTQ  D+
Sbjct: 121 KSGIILWTNHSLTVQLGWFRNLKSSLCTSTQECDK 153

BLAST of Lag0013888 vs. NCBI nr
Match: XP_038882472.1 (GDSL esterase/lipase At5g45910 isoform X2 [Benincasa hispida])

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-66
Identity = 129/155 (83.23%), Postives = 140/155 (90.32%), Query Frame = 0

Query: 1   MNSVSAFLCLCLISCSSALSKPYNAIFNFGDSLSDTGNFLRSGSLAFPVIGNLPYGQTFF 60
           MNS+ +FL L L  CSSALS PYNAIFNFGDSLSDTGNFLRSG+L+FPVIG LPYG+TFF
Sbjct: 1   MNSLLSFLFLSL--CSSALSTPYNAIFNFGDSLSDTGNFLRSGALSFPVIGKLPYGETFF 60

Query: 61  KHATGRCSDGRLVIDFIAEAFGLRYLPPYLGLKQGQNFSGGVNFAVAGATALDAEFFYKR 120
           +HATGRCSDGRLVIDFIAEAFGL +LPPYLGLKQGQ FSGGVNFAVAGATAL A+FFYKR
Sbjct: 61  RHATGRCSDGRLVIDFIAEAFGLPHLPPYLGLKQGQKFSGGVNFAVAGATALGADFFYKR 120

Query: 121 KTGTILWTNDSLSVQLGWFRKLKSSLCTSTQGVDR 156
           K GTILWTN SL+VQLGWFRKLKSSLCTS Q  ++
Sbjct: 121 KIGTILWTNHSLTVQLGWFRKLKSSLCTSAQECEK 153

BLAST of Lag0013888 vs. NCBI nr
Match: XP_038882471.1 (GDSL esterase/lipase At5g45910 isoform X1 [Benincasa hispida])

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-66
Identity = 128/155 (82.58%), Postives = 137/155 (88.39%), Query Frame = 0

Query: 1   MNSVSAFLCLCLISCSSALSKPYNAIFNFGDSLSDTGNFLRSGSLAFPVIGNLPYGQTFF 60
           MNS+ AFL LC   CSSALS+PYNAIFNFGDSLSDTGN LRS S A P IG LPYG+TFF
Sbjct: 1   MNSLLAFLFLCFCFCSSALSRPYNAIFNFGDSLSDTGNLLRSRSHAVPAIGKLPYGETFF 60

Query: 61  KHATGRCSDGRLVIDFIAEAFGLRYLPPYLGLKQGQNFSGGVNFAVAGATALDAEFFYKR 120
           +HATGRCSDGRLVIDFIAEAFGL +LPPYLGLKQGQNFS GVNFAVAGATALDA+FFYKR
Sbjct: 61  RHATGRCSDGRLVIDFIAEAFGLPHLPPYLGLKQGQNFSRGVNFAVAGATALDADFFYKR 120

Query: 121 KTGTILWTNDSLSVQLGWFRKLKSSLCTSTQGVDR 156
           K+G ILWTN SL+VQLGWFR LKSSLCTSTQ  D+
Sbjct: 121 KSGIILWTNHSLTVQLGWFRNLKSSLCTSTQECDK 155

BLAST of Lag0013888 vs. NCBI nr
Match: CAB4276212.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 231.1 bits (588), Expect = 7.9e-57
Identity = 111/149 (74.50%), Postives = 127/149 (85.23%), Query Frame = 0

Query: 6   AFLCLCLISCSSALSKPYNAIFNFGDSLSDTGNFLRSGSLAFPVIGNLPYGQTFFKHATG 65
           A LCL L + SS+L +PY AIFNFGDSLSDTGNFL SG+LAFPVIG LPYG+TFF+HATG
Sbjct: 8   ASLCLTLSTVSSSL-QPYEAIFNFGDSLSDTGNFLLSGALAFPVIGKLPYGETFFRHATG 67

Query: 66  RCSDGRLVIDFIAEAFGLRYLPPYLGLKQGQNFSGGVNFAVAGATALDAEFFYKRKTGTI 125
           RCSDGRLV+DFIAEA GL +LPPYL L +GQ+   GVNFAVAGATALD +FFY+RK G++
Sbjct: 68  RCSDGRLVVDFIAEALGLPHLPPYLALSKGQDVKHGVNFAVAGATALDPQFFYQRKIGSV 127

Query: 126 LWTNDSLSVQLGWFRKLKSSLCTSTQGVD 155
           LWTNDSLS QLGWF+KLK SLCT+ Q  D
Sbjct: 128 LWTNDSLSTQLGWFKKLKPSLCTTKQECD 155

BLAST of Lag0013888 vs. NCBI nr
Match: XP_021811462.1 (GDSL esterase/lipase At5g45910-like [Prunus avium])

HSP 1 Score: 229.2 bits (583), Expect = 3.0e-56
Identity = 110/149 (73.83%), Postives = 127/149 (85.23%), Query Frame = 0

Query: 6   AFLCLCLISCSSALSKPYNAIFNFGDSLSDTGNFLRSGSLAFPVIGNLPYGQTFFKHATG 65
           A LCL L + SS+L +PY AIFNFGDSLSDTGNFL SG+LAFPVIG LPYG+TFF+ ATG
Sbjct: 8   ASLCLTLSTVSSSL-QPYEAIFNFGDSLSDTGNFLLSGALAFPVIGKLPYGETFFRSATG 67

Query: 66  RCSDGRLVIDFIAEAFGLRYLPPYLGLKQGQNFSGGVNFAVAGATALDAEFFYKRKTGTI 125
           RCSDGRLV+DFIAEAFGL +LPPYL L +GQ+   GVNFAVAGATALD +FFY+RK G++
Sbjct: 68  RCSDGRLVVDFIAEAFGLPHLPPYLALSKGQDVKHGVNFAVAGATALDPQFFYQRKIGSV 127

Query: 126 LWTNDSLSVQLGWFRKLKSSLCTSTQGVD 155
           +WTNDSLS QLGWF+KLK SLCT+ Q  D
Sbjct: 128 MWTNDSLSTQLGWFKKLKPSLCTTKQECD 155

BLAST of Lag0013888 vs. ExPASy Swiss-Prot
Match: Q9FJ45 (GDSL esterase/lipase At5g45910 OS=Arabidopsis thaliana OX=3702 GN=At5g45910 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.5e-39
Identity = 84/142 (59.15%), Postives = 96/142 (67.61%), Query Frame = 0

Query: 12  LISCSSALSKP---YNAIFNFGDSLSDTGNFLRSGSLAFPVIGNLPYGQTFFKHATGRCS 71
           L+S  S   +P   Y +IFNFGDSLSDTGNFL SG +  P IG LPYGQTFF  +TGRCS
Sbjct: 14  LVSVRSLPMRPTLKYESIFNFGDSLSDTGNFLLSGDVDSPNIGRLPYGQTFFNRSTGRCS 73

Query: 72  DGRLVIDFIAEAFGLRYLPPY---LGLKQGQNFSGGVNFAVAGATALDAEFFYKRKTGTI 131
           DGRL+IDFIAEA GL Y+PPY   L      +F  G NFAVAGATA +  FF  R     
Sbjct: 74  DGRLIIDFIAEASGLPYIPPYLQSLRTNDSVDFKRGANFAVAGATANEFSFFKNRGLSVT 133

Query: 132 LWTNDSLSVQLGWFRKLKSSLC 148
           L TN +L +QL WF+KLK SLC
Sbjct: 134 LLTNKTLDIQLDWFKKLKPSLC 155

BLAST of Lag0013888 vs. ExPASy Swiss-Prot
Match: Q3ZFI4 (Sinapine esterase OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.2e-28
Identity = 71/146 (48.63%), Postives = 94/146 (64.38%), Query Frame = 0

Query: 7   FLCLCLISCSSALSKPYNAIFNFGDSLSDTGNFLRSGSLAF-PVIGNLPYGQTFFKHATG 66
           F    +++ S    + Y +I +FGDS++DTGN+L    +   P    LPYG+TFF   TG
Sbjct: 16  FFYTIIVASSEPSCRRYKSIISFGDSIADTGNYLHLSDVNHPPQAAFLPYGETFFSVPTG 75

Query: 67  RCSDGRLVIDFIAEAFGLRYLPPYLGLKQGQNFSGGVNFAVAGATALDAEFFYKRKTGTI 126
           R SDGRL+IDFIAE  GL Y+PPY G  Q  +F  GVNFAV GATALD  FF ++  G +
Sbjct: 76  RDSDGRLIIDFIAEFLGLPYVPPYFG-SQNVSFEQGVNFAVYGATALDRAFFIEK--GIV 135

Query: 127 L-WTNDSLSVQLGWFRKLKSSLCTST 151
             +TN SLSVQL  F+++  +LC S+
Sbjct: 136 SDFTNVSLSVQLNTFKQILPTLCASS 158

BLAST of Lag0013888 vs. ExPASy Swiss-Prot
Match: Q3E7I6 (GDSL esterase/lipase At1g28650 OS=Arabidopsis thaliana OX=3702 GN=At1g28650 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.0e-28
Identity = 66/140 (47.14%), Postives = 94/140 (67.14%), Query Frame = 0

Query: 12  LISCSSALSKPYNAIFNFGDSLSDTGNFLRSGSL-AFPVIGNLPYGQTFFKHATGRCSDG 71
           +++ S +  + Y +I +FGDS++DTGN++   ++   P    LPYG++FF   +GR SDG
Sbjct: 24  VVASSESRCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAFLPYGESFFHPPSGRYSDG 83

Query: 72  RLVIDFIAEAFGLRYLPPYLGLKQGQNFSGGVNFAVAGATALDAEFFYKRKTGTILWTND 131
           RLVIDFIAE  GL Y+PPY G  Q  +F+ G+NFAV GATALD  F  K+   +  +TN 
Sbjct: 84  RLVIDFIAEFLGLPYVPPYFG-SQNVSFNQGINFAVYGATALDRAFLVKQGIKSD-FTNI 143

Query: 132 SLSVQLGWFRKLKSSLCTST 151
           SLSVQL  F+++  +LC S+
Sbjct: 144 SLSVQLNTFKQILPNLCASS 161

BLAST of Lag0013888 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.0e-27
Identity = 68/128 (53.12%), Postives = 83/128 (64.84%), Query Frame = 0

Query: 23  YNAIFNFGDSLSDTGNFL-RSGSLAFPVIGNLPYGQTFFKHATGRCSDGRLVIDFIAEAF 82
           + +I +FGDS++DTGN +  S     PV    PYG+TFF H TGR  DGR+++DFIAE  
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFPPYGETFFHHPTGRSCDGRIIMDFIAEFV 88

Query: 83  GLRYLPPYLGLKQGQNFSGGVNFAVAGATALDAEFFYKRKTGTILWTNDSLSVQLGWFRK 142
           GL Y+PPY G K  +NF  GVNFAVAGATAL + F  KR  G    TN SL VQL  F+K
Sbjct: 89  GLPYVPPYFGSK-NRNFDKGVNFAVAGATALKSSFLKKR--GIQPHTNVSLGVQLKSFKK 148

Query: 143 LKSSLCTS 150
              +LC S
Sbjct: 149 SLPNLCGS 153

BLAST of Lag0013888 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.2e-27
Identity = 72/154 (46.75%), Postives = 93/154 (60.39%), Query Frame = 0

Query: 1   MNSVSAFLCLCLISCSSALSKP----YNAIFNFGDSLSDTGNFLR-SGSLAFPVIGNLPY 60
           M  VS FL L     ++  S+P    + +I +FGDS++DTGN L  S     P +  LPY
Sbjct: 6   MKLVSFFLILSTFCLTTVNSEPQCHNFKSIISFGDSIADTGNLLALSDPTNLPKVAFLPY 65

Query: 61  GQTFFKHATGRCSDGRLVIDFIAEAFGLRYLPPYLGLKQGQNFSGGVNFAVAGATALDAE 120
           G+TFF H TGR S+GRL+IDFIAE  G   +PP+ G  Q  NF  GVNFAV GATAL+  
Sbjct: 66  GETFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPFYG-SQNANFEKGVNFAVGGATALERS 125

Query: 121 FFYKRKTGTILWTNDSLSVQLGWFRKLKSSLCTS 150
           F  +R      +TN SL+VQL  F++   +LC S
Sbjct: 126 FLEERGI-HFPYTNVSLAVQLSSFKESLPNLCVS 157