Lag0013487 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013487
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr1: 50636799 .. 50640259 (+)
RNA-Seq ExpressionLag0013487
SyntenyLag0013487
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGATCAGTCATCAGCACCTCAAACTGGAAGACCCTTTTCTTCATAATTATAAGCCCTCAGAGCTACGGATCGCTTCCGAGTTTCTCACCACTTGGCTTCCCTTTCTTTCGAGAGATCTCTGCCGAGACTGCACCCAATTGCTCTCGGATCGAATCCGCGCCCTTGATCCAGGTATTCTTTTGTTGCTTTCTATTTGTTATCGGTCAATTATTGCACGAGCAGTGTTAGAGAAAAAAACCCATCTGATTTTTGTGACTCTTCAGAATAATTGAGCGTACGAAGGGATAAATTTACAACTTTAACGAGTTGGGGACTCGATTTTTTTTTTTTTTTATAAATATTATAATTGGTTAATTCGTATGCATATTTCTTTAAGCATTAGAGCATTGTGACTTCTTGATGTGTTGTGTTGCTAGAAAGAGTTAGGCGTCTGCCGAAATTTACATTATCTGTTCCTTTGGTTCGTAGGATTTAGATGCTTTTTATCTGTTTAATCATTAGGATTATCTAATGGAATTGTTGAACTTGACAATATTGTAGCTGTCCGTCCCAACGAGAATTCAAGTTCTGCACCACCCCCTATAGTGGAAGACCAAGACATGCATGAGAGTAATGGGAATCAGGATGATGCTTGTGATGCAAATTCTCTTGGAAGTTGGAAAGATGAAGCGGAAACGAATTCATTAGGGAGTTGGAAGGACGGCATAAATGAAGGGAATGAAGCTGATGGAGTACATGAAATTTCTTCTTGTGAACCATCCTCTAAATTAAATTCCACCAAAACCTCAGGGCCTCGAATGTCGTGGGCTGACATGACTCAGGAGGATGAACTAGAGGAAGAGGAAGATGAGTGTGAATCAGAAAAAAGATTAGTCAATGTTAATACGTCTACAGGGAAATTAACAATATCGAAGGTTATTGAGAAGCCAAAGCTGTCTAGAGAGCAGAGAGAGTACATCAGATTCATGAACGTTGGACGGAAAAAAGACTTTATATGTCTAGAGAGGTTTAAAGGAAAATTAGTAAACATTCTTGAAGGACTTGAGCTTCATACGGGTATTTTTAGTGCTGCAGAGCAGAAGAGAATAGTCGATCATGTTTATGCACTTCAAGAGATGGGCCAGAAGGGAGAATTAAGAGGTAATTATTCCCCTTCTCATATTGCTTTCTTTTATATTCTTCTTCTATCCTGCCATGTGTTGGTAAGCTTAATCTTTCCTCCCTCAAGTTTTAGTGCATTCGTTGAGTAAAGGTGTTTTGAAATGTTTTTCCTTGGATAATTTGGTTAATGGCTTTACTATTGATGCTCATCAACAAGCCACGCATCTGTAGTATCATTAATGTTGGTTCATGTGTAACCTAGGGACTCATCCTATTCTTGATTAGTCTTTTGTTTAGTATGTTATGTATAATGTACCTGAGGTCATGCAATATTTTGCATTAGTTCAAGTACAGTACAGTTTAAGGCTTCTAGCCTGTTATAATGTACCTAGTAATGAAATATTTTGTACTGGAAGGATTCTATCTGTGGTGCGGTTCATTAGAGAGTGTAGTATTAGCATTTGGAGCCTAGACTATAAGATGTTGGATGTTGTAGAATTGGTTGTACTAGATCTGGTAGATGGTAGAAGCAGAATAGAAAAGGCCAAAATCTATTCTTAGAAAGTTCTCAGTGCAAGTTCCACATAAGCAAACCAGATCATATGAGAGGGTCACACTCTCCCAAAACTACCTACTCAATTACTGCTTAAGTCTTTTTTTTTTTTTTTTTTTTGGAGAAAATTACTGCTTAAGTCATGCCTCGTTGCTTTCCCTTTTGAATGTACATTCTCTCTCTTTTAACTAACATGATATTCCCGTATTACTATTATCTTACATATTTATAAACTATCAGGTCTGTTGTAGGCTAGTAGTTCTCTTGGATTTATATCTTTAAATCCGTTCAGTAGACCCTGAGCTCATGCATCTTTGTTGTTATCCTTCTTAAGGATGCAAATAGTTTAGGGGAAACAAATGAGAATTGTGAGGATAGCTAACCAGTAGCTGAGCTTTTAATTTTGTTAAGTGTTATGGTTTTCTATCAAAAAGAAATTGTTAAATGGAATTCAAATAATTCTTCAACCAAATTACCAAGCAAATTTAACGTGCATTTTCTCTGCCTTCTTTGGTTAAAAATTTTAATCTCTATTTTAGATTTAGCCAGAACACATGATAATGTTACTCCATAGTCCCCATTTTTGGTTTCAACTTCTTCGTTTTTCATCAAGTACAAACATGACAATGACTTGAACGCAATGCCAAACTCACCATTAATTTTTTTTTTCTTCAGAACGAACATTTTCAGCTCCCAAGAAGTGGATGAAAGGAAAGGGACGTGTAACTCTGCAGTTTGGGTGCTGTTACAATTATGCACCTGTGAGTCTATCTGATTGCTAAGTTGGTGTGATTCTTTTGAATTCCATCTAATTTTTCTGTTTTTGATCTTCAACCAATTGTGATGCTCAGCTAGTATTTCTAATTAGAGTATCATGTTGAATGAATGGGCAAATCATTCTAATAAAAATTCTGGTGACAGGATAAAAATGGCAATCCTCCTGGAATTCTTCGAAGTGAAATTGTGGATCCAATACCTTCTCTCTTTAAGGTGATCATTAGAAGGTTGGTGAGATGGCATGTGCTTCCTCCAACATGTGTTCCTGATAGTTGCATTGTGAACATCTATGAAGAAGGAGACTGTATTCCTCCCCATATTGACAACCATGATTTTGTTCGACCTTTTTGCACTGTCTCATTCCTGAGTGAATGCAATATTCTTTTTGGAACAAACCTTTCTATTGTAGGACCTGGTGAATTTTCTGGGCCAATGGCAATCCCTCTGCCTGTGGGGTACGTATAGTATGCAATCAACTATGTTCATTTTTGTGGTTCCATCTCTGCAAGTTATCAATTGCATTAGATTTCAAATCCTTATTGTTATCTATTTTCTTGCAGATCTGTTCTTGTGTTAAATGGAAATGGAGCTGATGTTGCTAAACATTGTGTTCCTGCAGTCCCTATGAAGAGGTAAAGTTCAGGGAGGTCGCTTGAATATAATTCTATTAGGCCAAGACTTTTACTTGCTTAATGTTATCCAATCTAACTTGCCTCTTTTCTGGTCTATTTTAGGATATCAATTACATTTAGAAGGATAGATGAGTCCAAAAGGCCGATTGAGTATGCTTCAGAACCGGATTTGCAGGGAATCCAACCATTGCCCTATGAAGCAAGTGAAAATGATGTACCAACTTCTCCTATATCATCAGAAAGAGAAATGAGGAGGCAGCCATTTAGGAGGGGTAGTCAAATGAGAACCAGGGGATCTGGCAATAGAAGCGACAATCGATTTGAGCCACGCAATCCAGGTAGGGCTCAACATAGGTCAGCAGATAGGAGGAGCAGAGGAAATCTAGACAGCTAA

mRNA sequence

ATGCCGATCAGTCATCAGCACCTCAAACTGGAAGACCCTTTTCTTCATAATTATAAGCCCTCAGAGCTACGGATCGCTTCCGAGTTTCTCACCACTTGGCTTCCCTTTCTTTCGAGAGATCTCTGCCGAGACTGCACCCAATTGCTCTCGGATCGAATCCGCGCCCTTGATCCAGCTGTCCGTCCCAACGAGAATTCAAGTTCTGCACCACCCCCTATAGTGGAAGACCAAGACATGCATGAGAGTAATGGGAATCAGGATGATGCTTGTGATGCAAATTCTCTTGGAAGTTGGAAAGATGAAGCGGAAACGAATTCATTAGGGAGTTGGAAGGACGGCATAAATGAAGGGAATGAAGCTGATGGAGTACATGAAATTTCTTCTTGTGAACCATCCTCTAAATTAAATTCCACCAAAACCTCAGGGCCTCGAATGTCGTGGGCTGACATGACTCAGGAGGATGAACTAGAGGAAGAGGAAGATGAGTGTGAATCAGAAAAAAGATTAGTCAATGTTAATACGTCTACAGGGAAATTAACAATATCGAAGGTTATTGAGAAGCCAAAGCTGTCTAGAGAGCAGAGAGAGTACATCAGATTCATGAACGTTGGACGGAAAAAAGACTTTATATGTCTAGAGAGGTTTAAAGGAAAATTAGTAAACATTCTTGAAGGACTTGAGCTTCATACGGGTATTTTTAGTGCTGCAGAGCAGAAGAGAATAGTCGATCATGTTTATGCACTTCAAGAGATGGGCCAGAAGGGAGAATTAAGAGAACGAACATTTTCAGCTCCCAAGAAGTGGATGAAAGGAAAGGGACGTGTAACTCTGCAGTTTGGGTGCTGTTACAATTATGCACCTGATAAAAATGGCAATCCTCCTGGAATTCTTCGAAGTGAAATTGTGGATCCAATACCTTCTCTCTTTAAGGTGATCATTAGAAGGTTGGTGAGATGGCATGTGCTTCCTCCAACATGTGTTCCTGATAGTTGCATTGTGAACATCTATGAAGAAGGAGACTGTATTCCTCCCCATATTGACAACCATGATTTTGTTCGACCTTTTTGCACTGTCTCATTCCTGAGTGAATGCAATATTCTTTTTGGAACAAACCTTTCTATTGTAGGACCTGGTGAATTTTCTGGGCCAATGGCAATCCCTCTGCCTGTGGGATCTGTTCTTGTGTTAAATGGAAATGGAGCTGATGTTGCTAAACATTGTGTTCCTGCAGTCCCTATGAAGAGGATATCAATTACATTTAGAAGGATAGATGAGTCCAAAAGGCCGATTGAGTATGCTTCAGAACCGGATTTGCAGGGAATCCAACCATTGCCCTATGAAGCAAGTGAAAATGATGTACCAACTTCTCCTATATCATCAGAAAGAGAAATGAGGAGGCAGCCATTTAGGAGGGGTAGTCAAATGAGAACCAGGGGATCTGGCAATAGAAGCGACAATCGATTTGAGCCACGCAATCCAGGTAGGGCTCAACATAGGTCAGCAGATAGGAGGAGCAGAGGAAATCTAGACAGCTAA

Coding sequence (CDS)

ATGCCGATCAGTCATCAGCACCTCAAACTGGAAGACCCTTTTCTTCATAATTATAAGCCCTCAGAGCTACGGATCGCTTCCGAGTTTCTCACCACTTGGCTTCCCTTTCTTTCGAGAGATCTCTGCCGAGACTGCACCCAATTGCTCTCGGATCGAATCCGCGCCCTTGATCCAGCTGTCCGTCCCAACGAGAATTCAAGTTCTGCACCACCCCCTATAGTGGAAGACCAAGACATGCATGAGAGTAATGGGAATCAGGATGATGCTTGTGATGCAAATTCTCTTGGAAGTTGGAAAGATGAAGCGGAAACGAATTCATTAGGGAGTTGGAAGGACGGCATAAATGAAGGGAATGAAGCTGATGGAGTACATGAAATTTCTTCTTGTGAACCATCCTCTAAATTAAATTCCACCAAAACCTCAGGGCCTCGAATGTCGTGGGCTGACATGACTCAGGAGGATGAACTAGAGGAAGAGGAAGATGAGTGTGAATCAGAAAAAAGATTAGTCAATGTTAATACGTCTACAGGGAAATTAACAATATCGAAGGTTATTGAGAAGCCAAAGCTGTCTAGAGAGCAGAGAGAGTACATCAGATTCATGAACGTTGGACGGAAAAAAGACTTTATATGTCTAGAGAGGTTTAAAGGAAAATTAGTAAACATTCTTGAAGGACTTGAGCTTCATACGGGTATTTTTAGTGCTGCAGAGCAGAAGAGAATAGTCGATCATGTTTATGCACTTCAAGAGATGGGCCAGAAGGGAGAATTAAGAGAACGAACATTTTCAGCTCCCAAGAAGTGGATGAAAGGAAAGGGACGTGTAACTCTGCAGTTTGGGTGCTGTTACAATTATGCACCTGATAAAAATGGCAATCCTCCTGGAATTCTTCGAAGTGAAATTGTGGATCCAATACCTTCTCTCTTTAAGGTGATCATTAGAAGGTTGGTGAGATGGCATGTGCTTCCTCCAACATGTGTTCCTGATAGTTGCATTGTGAACATCTATGAAGAAGGAGACTGTATTCCTCCCCATATTGACAACCATGATTTTGTTCGACCTTTTTGCACTGTCTCATTCCTGAGTGAATGCAATATTCTTTTTGGAACAAACCTTTCTATTGTAGGACCTGGTGAATTTTCTGGGCCAATGGCAATCCCTCTGCCTGTGGGATCTGTTCTTGTGTTAAATGGAAATGGAGCTGATGTTGCTAAACATTGTGTTCCTGCAGTCCCTATGAAGAGGATATCAATTACATTTAGAAGGATAGATGAGTCCAAAAGGCCGATTGAGTATGCTTCAGAACCGGATTTGCAGGGAATCCAACCATTGCCCTATGAAGCAAGTGAAAATGATGTACCAACTTCTCCTATATCATCAGAAAGAGAAATGAGGAGGCAGCCATTTAGGAGGGGTAGTCAAATGAGAACCAGGGGATCTGGCAATAGAAGCGACAATCGATTTGAGCCACGCAATCCAGGTAGGGCTCAACATAGGTCAGCAGATAGGAGGAGCAGAGGAAATCTAGACAGCTAA

Protein sequence

MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAVRPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEADGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLTISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMRTRGSGNRSDNRFEPRNPGRAQHRSADRRSRGNLDS
Homology
BLAST of Lag0013487 vs. NCBI nr
Match: XP_038882153.1 (RNA demethylase ALKBH9B [Benincasa hispida])

HSP 1 Score: 926.8 bits (2394), Expect = 8.2e-266
Identity = 462/506 (91.30%), Postives = 479/506 (94.66%), Query Frame = 0

Query: 1   MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAV 60
           M ISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCT+LLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTKLLSDRIRALDPAV 60

Query: 61  RPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120
           R  EN  +APP     +DMHESNGNQDDACDANSLGSWKDE ETNSLGSWKDGINEGNEA
Sbjct: 61  RSGENFGAAPPV----EDMHESNGNQDDACDANSLGSWKDEGETNSLGSWKDGINEGNEA 120

Query: 121 DGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLT 180
           D V E SS E SSKLNSTKTSGPRMSWADMTQEDELEEE+DE ESEKRLV++N ST KLT
Sbjct: 121 DVVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEDDEYESEKRLVSINESTRKLT 180

Query: 181 ISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR 240
           ISKVIEKP LSREQRE+IRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR
Sbjct: 181 ISKVIEKPTLSREQREHIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR 240

Query: 241 IVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSE 300
           IVDHVYALQEMG+KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSE
Sbjct: 241 IVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSE 300

Query: 301 IVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSF 360
           IVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIY+EGDCIPPHIDNHDFVRPFCTVSF
Sbjct: 301 IVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSF 360

Query: 361 LSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITF 420
           LSECNI+FG+NLSIVGPGEFSGP+AIPLPVGSVLVLNGNGADVAKHCVPAVP KRISITF
Sbjct: 361 LSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITF 420

Query: 421 RRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMRTRGS 480
           RRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSP+SSERE+RRQPFRRG  MR RGS
Sbjct: 421 RRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRRGGHMRNRGS 480

Query: 481 GNRSDNRFEPRNPGRAQHRSADRRSR 507
           GNRSD RF+PRNPGR +H SADRR+R
Sbjct: 481 GNRSDTRFDPRNPGRGRHSSADRRTR 502

BLAST of Lag0013487 vs. NCBI nr
Match: XP_022926828.1 (uncharacterized protein LOC111433825 [Cucurbita moschata])

HSP 1 Score: 915.2 bits (2364), Expect = 2.5e-262
Identity = 458/511 (89.63%), Postives = 482/511 (94.32%), Query Frame = 0

Query: 1   MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAV 60
           M ISHQHLKLEDPFL NYKPSELRIASEFLTTWLPFLSRDLC+DCTQLLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLQNYKPSELRIASEFLTTWLPFLSRDLCQDCTQLLSDRIRALDPAV 60

Query: 61  RPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120
           RPNE+SSSAPPP   ++DM ESNGN+ DACDANSLGSWKDEAETNSLGSW+DGINEGNEA
Sbjct: 61  RPNEDSSSAPPP--REKDMSESNGNEVDACDANSLGSWKDEAETNSLGSWQDGINEGNEA 120

Query: 121 DGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLT 180
           DG+ E SS EPSSKLNSTKTSGPR+SWADMTQEDELEEEEDECE+EKRLVN N   GKLT
Sbjct: 121 DGLPETSSSEPSSKLNSTKTSGPRLSWADMTQEDELEEEEDECETEKRLVNAN--AGKLT 180

Query: 181 ISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR 240
           ISKVIEKPKLSREQREYIRFM+VGRKKDFICLERFKGK VNILEGLELHTGIFSAAEQKR
Sbjct: 181 ISKVIEKPKLSREQREYIRFMSVGRKKDFICLERFKGKFVNILEGLELHTGIFSAAEQKR 240

Query: 241 IVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSE 300
           IVDHVYALQEMG KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYA DKNGNPPGIL+SE
Sbjct: 241 IVDHVYALQEMGMKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAADKNGNPPGILQSE 300

Query: 301 IVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSF 360
           IVD IPSLFKVIIRRLVRWHV+PPTCVPDSCIVNIY+EGDCIPPHIDNHDFVRPFCTVSF
Sbjct: 301 IVDSIPSLFKVIIRRLVRWHVIPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSF 360

Query: 361 LSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITF 420
           LSECNI+FGTNLSIVGPG+FSGP+AIPLPVGSVLVLNGN ADVA+HCVPAVP KRISITF
Sbjct: 361 LSECNIVFGTNLSIVGPGQFSGPIAIPLPVGSVLVLNGNSADVARHCVPAVPTKRISITF 420

Query: 421 RRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRR-GSQMRTRG 480
           RRIDE KRPIEYA EPDLQGIQPLPYEAS+NDVPTSP+SSERE+RRQPFRR G  MRTRG
Sbjct: 421 RRIDEPKRPIEYAPEPDLQGIQPLPYEASKNDVPTSPVSSEREIRRQPFRRGGGHMRTRG 480

Query: 481 SGNRSDNRFEPRNPGRAQHRSADRRSRGNLD 511
           SGNRS+  FEPRNPGRA+H S+DRRSR NL+
Sbjct: 481 SGNRSNTLFEPRNPGRARHTSSDRRSRVNLE 507

BLAST of Lag0013487 vs. NCBI nr
Match: XP_023518278.1 (uncharacterized protein LOC111781804 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 913.7 bits (2360), Expect = 7.2e-262
Identity = 456/511 (89.24%), Postives = 484/511 (94.72%), Query Frame = 0

Query: 1   MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAV 60
           M ISHQHLKLEDPFL NYKPSELRIASEFLTTWLPFLSRDLC+DCTQLLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLQNYKPSELRIASEFLTTWLPFLSRDLCQDCTQLLSDRIRALDPAV 60

Query: 61  RPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120
            P+E+SSSAPP  + ++ M+ESNGN+DDACDANSLGSWKDEAETNSLGSW+DGINEGNEA
Sbjct: 61  HPDEDSSSAPP--LREKAMNESNGNEDDACDANSLGSWKDEAETNSLGSWQDGINEGNEA 120

Query: 121 DGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLT 180
           DG+ E SS EPSSKLNSTKTSGPR+SWADMTQEDELEEEEDECE+EKRLVN N   GKLT
Sbjct: 121 DGLPETSSSEPSSKLNSTKTSGPRLSWADMTQEDELEEEEDECETEKRLVNAN--AGKLT 180

Query: 181 ISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR 240
           ISKVIEKPKLSREQREYIRFM+VGRKKDFICLERFKGK VNILEGLELHTGIFSAAEQKR
Sbjct: 181 ISKVIEKPKLSREQREYIRFMSVGRKKDFICLERFKGKFVNILEGLELHTGIFSAAEQKR 240

Query: 241 IVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSE 300
           IVDHVYALQEMG KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYA DKNGNPPGIL+SE
Sbjct: 241 IVDHVYALQEMGMKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAADKNGNPPGILQSE 300

Query: 301 IVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSF 360
           IVD IPSLFKVIIRRLVRWHV+PPTCVPDSCIVNIY+EGDCIPPHIDNHDFVRPFCTVSF
Sbjct: 301 IVDSIPSLFKVIIRRLVRWHVIPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSF 360

Query: 361 LSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITF 420
           LSECNI+FGTNLSIVGPG+FSGP+AIPLPVGSVLVLNGN ADVA+HCVPAVP KRISITF
Sbjct: 361 LSECNIVFGTNLSIVGPGQFSGPIAIPLPVGSVLVLNGNSADVARHCVPAVPTKRISITF 420

Query: 421 RRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRR-GSQMRTRG 480
           RRIDESKRPIEYA EPDLQGIQPLPYEAS+NDVPTSP+SSERE+RRQPFRR G  MRTRG
Sbjct: 421 RRIDESKRPIEYAPEPDLQGIQPLPYEASKNDVPTSPVSSEREIRRQPFRRGGGHMRTRG 480

Query: 481 SGNRSDNRFEPRNPGRAQHRSADRRSRGNLD 511
           SGNRSD +FEPRNPGRA++ S+DRRSR NL+
Sbjct: 481 SGNRSDTQFEPRNPGRARYTSSDRRSRVNLE 507

BLAST of Lag0013487 vs. NCBI nr
Match: KAG6594779.1 (RNA demethylase ALKBH9B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026744.1 RNA demethylase ALKBH5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 908.3 bits (2346), Expect = 3.0e-260
Identity = 454/511 (88.85%), Postives = 480/511 (93.93%), Query Frame = 0

Query: 1   MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAV 60
           M ISHQHLKLEDPFL NYKPSELRIASEFLTTWLPFLSRDLC+DCTQLLSDRIRA+DPAV
Sbjct: 1   MAISHQHLKLEDPFLQNYKPSELRIASEFLTTWLPFLSRDLCQDCTQLLSDRIRAIDPAV 60

Query: 61  RPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120
           RPNE+SSSAPPP   ++D  +SNGN+ DACDANSLGSWKDEAETNSLGSW+DGINEGNEA
Sbjct: 61  RPNEDSSSAPPP--REKDTSQSNGNEVDACDANSLGSWKDEAETNSLGSWQDGINEGNEA 120

Query: 121 DGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLT 180
           DG+ E SS EPSSKLNSTKTSGPR+SWADMTQEDELEEEEDECE+EKRLVN N   GKLT
Sbjct: 121 DGLPETSSSEPSSKLNSTKTSGPRLSWADMTQEDELEEEEDECETEKRLVNAN--AGKLT 180

Query: 181 ISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR 240
           ISKVI KPKLSREQREYIRFM+VGRKKDFICLERFKGK VNILEGLELHTGIFSAAEQKR
Sbjct: 181 ISKVIGKPKLSREQREYIRFMSVGRKKDFICLERFKGKFVNILEGLELHTGIFSAAEQKR 240

Query: 241 IVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSE 300
           IVDHVYALQEMG KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYA DKNGNPPGIL+SE
Sbjct: 241 IVDHVYALQEMGMKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAADKNGNPPGILQSE 300

Query: 301 IVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSF 360
           IVD IPSLFKVIIRRLVRWHV+PPTCVPDSCIVNIY+EGDCIPPHIDNHDFVRPFCTVSF
Sbjct: 301 IVDSIPSLFKVIIRRLVRWHVIPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSF 360

Query: 361 LSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITF 420
           LSECNI+FGTNLSIVGPG+FSGP+AIPLPVGSVLVLNGN ADVA+HCVPAVP KRISITF
Sbjct: 361 LSECNIVFGTNLSIVGPGQFSGPIAIPLPVGSVLVLNGNSADVARHCVPAVPTKRISITF 420

Query: 421 RRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRR-GSQMRTRG 480
           RRIDE KRPIEYA EPDLQGIQPLPYEAS+NDVPTSP+SSERE+RRQPFRR G  MRTRG
Sbjct: 421 RRIDEPKRPIEYAPEPDLQGIQPLPYEASKNDVPTSPVSSEREIRRQPFRRGGGHMRTRG 480

Query: 481 SGNRSDNRFEPRNPGRAQHRSADRRSRGNLD 511
           SGNRS+  FEPRNPGRA+H S+DRRSR NL+
Sbjct: 481 SGNRSNTLFEPRNPGRARHTSSDRRSRVNLE 507

BLAST of Lag0013487 vs. NCBI nr
Match: XP_023544551.1 (uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo] >XP_023544552.1 uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo] >XP_023544553.1 uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907.1 bits (2343), Expect = 6.7e-260
Identity = 455/507 (89.74%), Postives = 479/507 (94.48%), Query Frame = 0

Query: 1   MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAV 60
           M ISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCR+CT+LLSDRIRALDPA 
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDPAA 60

Query: 61  RPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120
           R +ENSSS PP  V    MHESNGNQ+DACDANSLGSWKDEAETNSLGSWKDGINEGNEA
Sbjct: 61  RTDENSSSTPPVEV----MHESNGNQNDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120

Query: 121 DGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDEL-EEEEDECESEKRLVNVNTSTGKL 180
           DGV E SS  PSSK+ STKTSGPRMSWADMTQEDEL EEEEDE ESEKR+V+VN ST KL
Sbjct: 121 DGVPETSSSIPSSKVVSTKTSGPRMSWADMTQEDELEEEEEDEYESEKRVVSVNESTRKL 180

Query: 181 TISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQK 240
           TISKVIEKPKLSREQRE+IRF+NVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQK
Sbjct: 181 TISKVIEKPKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQK 240

Query: 241 RIVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRS 300
           RIVDH+Y+LQEMG+KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRS
Sbjct: 241 RIVDHIYSLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRS 300

Query: 301 EIVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVS 360
           EIVDPIPSLFKVIIRRLVRWHVLPPTCVP+SCIVNIY+EGDCIPPHID+HDFVRPFCTVS
Sbjct: 301 EIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYDEGDCIPPHIDSHDFVRPFCTVS 360

Query: 361 FLSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISIT 420
           FLSECNI+FGTNLSIVGPGEFSGP+AIPLPVGSVLVLNGNGADVAKHCVPAVP KRISIT
Sbjct: 361 FLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISIT 420

Query: 421 FRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMRTRG 480
           FRR+D SK PIEYA EPDLQGIQPLPYEASEN+VPTSP+SSERE+RRQPFRRGS MRTRG
Sbjct: 421 FRRMDGSKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQPFRRGSHMRTRG 480

Query: 481 SGNRSDNRFEPRNPGRAQHRSADRRSR 507
           SGNR+D RF+ RNPGR +H SADRRSR
Sbjct: 481 SGNRTDTRFDSRNPGRGRHSSADRRSR 503

BLAST of Lag0013487 vs. ExPASy Swiss-Prot
Match: Q9SL49 (RNA demethylase ALKBH9B OS=Arabidopsis thaliana OX=3702 GN=ALKBH9B PE=1 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.9e-156
Identity = 295/518 (56.95%), Postives = 360/518 (69.50%), Query Frame = 0

Query: 12  DPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAVRPNENS----- 71
           DPFL  Y+PSEL+IASEFLT WLPFLS+DLC+DC  LLS+RIR+LDPA   N        
Sbjct: 4   DPFLRQYQPSELKIASEFLTNWLPFLSKDLCKDCNHLLSNRIRSLDPAHCSNNTDKVDGE 63

Query: 72  -SSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDG-INEGNEADGVH 131
             +    +VE+     ++ N DD  D  S     ++ + +SLGSWK   I  G+  +   
Sbjct: 64  CKTGSCSVVENMGSERASNNVDDNYDEKSENG--EDCDNHSLGSWKGSEIVFGSFPEDFS 123

Query: 132 EISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLTISKV 191
            +    P+      +T+ PRM WADM QEDE +EEE+E E E+           +   K 
Sbjct: 124 SVLQSRPA----VVETASPRMRWADMAQEDEFDEEEEEEEEERDSSRKGFDASSM---KT 183

Query: 192 IEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDH 251
            EKPKLSR+QRE +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD 
Sbjct: 184 PEKPKLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQ 243

Query: 252 VYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIVDP 311
           VY LQE G++GEL++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPGIL+ E VDP
Sbjct: 244 VYQLQEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDP 303

Query: 312 IPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSEC 371
           +P LFKVIIR+L++WHVLPPTCVPDSCIVNIY+EGDCIPPHIDNHDF+RPFCT+SFLSEC
Sbjct: 304 LPHLFKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSEC 363

Query: 372 NILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITFRRID 431
           +ILFG+NL + GPG+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVP KRISITFR++D
Sbjct: 364 DILFGSNLKVEGPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMD 423

Query: 432 ESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMR---TRGSG 491
           ESKRP+ +  EPDLQGI+PLP + + +        S     R     G+  R    RG G
Sbjct: 424 ESKRPVWFTPEPDLQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGG 483

Query: 492 NRSDNR--FEPRNPGR---------AQHRSADRRSRGN 509
           N  D+R  + P              +Q R   R SR N
Sbjct: 484 NGYDSRGYYNPERSSEHNDSGDWPSSQRRGMPRPSRRN 505

BLAST of Lag0013487 vs. ExPASy Swiss-Prot
Match: Q9ZT92 (RNA demethylase ALKBH10B OS=Arabidopsis thaliana OX=3702 GN=ALKBH10B PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.5e-28
Identity = 75/219 (34.25%), Postives = 116/219 (52.97%), Query Frame = 0

Query: 207 KDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGQKGELRERTFSAPK 266
           K F   E+ KG  VN+++GL+L+  +    E  +++D V  L+E G  G+L   +F    
Sbjct: 223 KGFQAKEQVKGHTVNVVKGLKLYEELLKEDEISKLLDFVAELREAGINGKLAGESFILFN 282

Query: 267 KWMKGKGRVTLQFGCCY--NYAPDKNGNPPGILRSEIVDPIPSLFKVIIRRLVRWHVLPP 326
           K +KG  R  +Q G     +   D+N N      S  ++PIP L + +I   V W ++P 
Sbjct: 283 KQIKGNKRELIQLGVPIFGHVKADENSNDTN--NSVNIEPIPPLLESVIDHFVTWRLIPE 342

Query: 327 TCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNILFGTNLSIVGPGEFSGPM 386
              P+ C++N +EEG+   P +      +P  T+  LSE  + +G  LS    G F GP+
Sbjct: 343 YKRPNGCVINFFEEGEYSQPFLKPPHLEQPISTL-VLSESTMAYGRILSSDNEGNFRGPL 402

Query: 387 AIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITFRRI 424
            + L  GS+LV+ GN AD+A+H +     KR+SITF RI
Sbjct: 403 TLSLKQGSLLVMRGNSADMARHVMCPSQNKRVSITFFRI 438

BLAST of Lag0013487 vs. ExPASy Swiss-Prot
Match: Q66JG8 (RNA demethylase ALKBH5 OS=Xenopus tropicalis OX=8364 GN=alkbh5 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 8.8e-13
Identity = 67/208 (32.21%), Postives = 98/208 (47.12%), Query Frame = 0

Query: 232 IFSAAEQKRIVDHVYALQEMGQKGELRERTFS-AP--KKWMKGKGRVTLQFGCCYNYAPD 291
           +FS  E  RI   +  +    +KG  RE T   AP   K+  G+G         Y Y   
Sbjct: 60  LFSPDECARIEAKIDEVVSRAEKGLYREHTVDRAPLRNKYFFGEG---------YTYGAQ 119

Query: 292 KNGNPPG---ILRSEIVDPIPS-LFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPP 351
                PG   +     VD IP+ + +++IRRLV   V+P   V +S ++N Y+ G CI  
Sbjct: 120 LQRRGPGQERLYPKGEVDEIPAWVNELVIRRLVEHRVIPEGFV-NSAVINDYQPGGCIVS 179

Query: 352 HIDN-HDFVRPFCTVSFLSECNILFGTNLSIVGPGEFSGPM-AIPLPVGSVLVLNGNGAD 411
           H+D  H F RP  +VSF S+  + FG       P   S P+  +P+  GSV VL+G  AD
Sbjct: 180 HVDPIHIFERPIVSVSFFSDSALCFGCKFQF-KPIRVSEPVFFLPVQRGSVTVLSGYAAD 239

Query: 412 VAKHCV-PAVPMKRISITFRRIDESKRP 430
              HC+ P    +R ++   R   ++ P
Sbjct: 240 EITHCIRPQDIKERRAVVILRKTRTEAP 256

BLAST of Lag0013487 vs. ExPASy TrEMBL
Match: A0A6J1EJB3 (uncharacterized protein LOC111433825 OS=Cucurbita moschata OX=3662 GN=LOC111433825 PE=4 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 1.2e-262
Identity = 458/511 (89.63%), Postives = 482/511 (94.32%), Query Frame = 0

Query: 1   MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAV 60
           M ISHQHLKLEDPFL NYKPSELRIASEFLTTWLPFLSRDLC+DCTQLLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLQNYKPSELRIASEFLTTWLPFLSRDLCQDCTQLLSDRIRALDPAV 60

Query: 61  RPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120
           RPNE+SSSAPPP   ++DM ESNGN+ DACDANSLGSWKDEAETNSLGSW+DGINEGNEA
Sbjct: 61  RPNEDSSSAPPP--REKDMSESNGNEVDACDANSLGSWKDEAETNSLGSWQDGINEGNEA 120

Query: 121 DGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLT 180
           DG+ E SS EPSSKLNSTKTSGPR+SWADMTQEDELEEEEDECE+EKRLVN N   GKLT
Sbjct: 121 DGLPETSSSEPSSKLNSTKTSGPRLSWADMTQEDELEEEEDECETEKRLVNAN--AGKLT 180

Query: 181 ISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR 240
           ISKVIEKPKLSREQREYIRFM+VGRKKDFICLERFKGK VNILEGLELHTGIFSAAEQKR
Sbjct: 181 ISKVIEKPKLSREQREYIRFMSVGRKKDFICLERFKGKFVNILEGLELHTGIFSAAEQKR 240

Query: 241 IVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSE 300
           IVDHVYALQEMG KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYA DKNGNPPGIL+SE
Sbjct: 241 IVDHVYALQEMGMKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAADKNGNPPGILQSE 300

Query: 301 IVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSF 360
           IVD IPSLFKVIIRRLVRWHV+PPTCVPDSCIVNIY+EGDCIPPHIDNHDFVRPFCTVSF
Sbjct: 301 IVDSIPSLFKVIIRRLVRWHVIPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSF 360

Query: 361 LSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITF 420
           LSECNI+FGTNLSIVGPG+FSGP+AIPLPVGSVLVLNGN ADVA+HCVPAVP KRISITF
Sbjct: 361 LSECNIVFGTNLSIVGPGQFSGPIAIPLPVGSVLVLNGNSADVARHCVPAVPTKRISITF 420

Query: 421 RRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRR-GSQMRTRG 480
           RRIDE KRPIEYA EPDLQGIQPLPYEAS+NDVPTSP+SSERE+RRQPFRR G  MRTRG
Sbjct: 421 RRIDEPKRPIEYAPEPDLQGIQPLPYEASKNDVPTSPVSSEREIRRQPFRRGGGHMRTRG 480

Query: 481 SGNRSDNRFEPRNPGRAQHRSADRRSRGNLD 511
           SGNRS+  FEPRNPGRA+H S+DRRSR NL+
Sbjct: 481 SGNRSNTLFEPRNPGRARHTSSDRRSRVNLE 507

BLAST of Lag0013487 vs. ExPASy TrEMBL
Match: A0A6J1KUK5 (uncharacterized protein LOC111497336 OS=Cucurbita maxima OX=3661 GN=LOC111497336 PE=4 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.5e-260
Identity = 454/511 (88.85%), Postives = 481/511 (94.13%), Query Frame = 0

Query: 1   MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAV 60
           M ISHQHLKLEDPFL NYKPSELRIASEFLTTWLPFLSRDLC+DCTQLLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLQNYKPSELRIASEFLTTWLPFLSRDLCQDCTQLLSDRIRALDPAV 60

Query: 61  RPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120
           RP+E+SSSA PP   ++DM+ESNGN+ DACDANSLGSWKDEAETNSLGSW+DGINEGNEA
Sbjct: 61  RPDEDSSSASPP--REKDMNESNGNEVDACDANSLGSWKDEAETNSLGSWQDGINEGNEA 120

Query: 121 DGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLT 180
           DG+ E SS EPSSKLNSTKTSGPR+SWADMTQEDELEEEEDECE+EKR+VN N   GKLT
Sbjct: 121 DGLPETSSSEPSSKLNSTKTSGPRLSWADMTQEDELEEEEDECETEKRIVNAN--AGKLT 180

Query: 181 ISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR 240
           ISKVIEKPKLSREQREYIRFM+VGRKKDFICLERFKGK VNILEGLELHTGIFSAAEQKR
Sbjct: 181 ISKVIEKPKLSREQREYIRFMSVGRKKDFICLERFKGKFVNILEGLELHTGIFSAAEQKR 240

Query: 241 IVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSE 300
           IVDHVYALQEMG KGEL+ERTFSAPKKWMKGKGRVTLQFGCCYNYA DKNGNPPGIL+SE
Sbjct: 241 IVDHVYALQEMGMKGELKERTFSAPKKWMKGKGRVTLQFGCCYNYAADKNGNPPGILQSE 300

Query: 301 IVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSF 360
           IVD IPSLFKVIIRRLVRWHV+PPTCVPDSCIVNIY+E DCIPPHIDNHDFVRPFCTVSF
Sbjct: 301 IVDSIPSLFKVIIRRLVRWHVIPPTCVPDSCIVNIYDEEDCIPPHIDNHDFVRPFCTVSF 360

Query: 361 LSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITF 420
           LSECNI+FGTNLSIVGPG+FSGP+AIPLPVGSVLVLNGN ADVA+HCVPAVP KRISITF
Sbjct: 361 LSECNIVFGTNLSIVGPGQFSGPIAIPLPVGSVLVLNGNSADVARHCVPAVPTKRISITF 420

Query: 421 RRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRR-GSQMRTRG 480
           RRIDESKRPIEYA EPDLQGIQPLPYEAS+NDVPTS +SSERE+RRQPFRR G  MRTRG
Sbjct: 421 RRIDESKRPIEYAPEPDLQGIQPLPYEASKNDVPTSHVSSEREIRRQPFRRGGGHMRTRG 480

Query: 481 SGNRSDNRFEPRNPGRAQHRSADRRSRGNLD 511
           SGNRSD +FEPRNPGRA H S+DRRSR NL+
Sbjct: 481 SGNRSDTQFEPRNPGRAWHTSSDRRSRVNLE 507

BLAST of Lag0013487 vs. ExPASy TrEMBL
Match: A0A6J1BY69 (uncharacterized protein LOC111005802 OS=Momordica charantia OX=3673 GN=LOC111005802 PE=4 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.0e-259
Identity = 453/510 (88.82%), Postives = 474/510 (92.94%), Query Frame = 0

Query: 3   ISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAVRP 62
           ++HQHL+LED FL NYKPSE RIASEFLTTWLPFLSRDLC DCTQLLSDRIRALDPAV  
Sbjct: 1   MTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHS 60

Query: 63  NENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEADG 122
           +E SSS PP +   +DMH SNGNQDD CD NSLGSWKDEAETNSLGSWKD INEG EA+G
Sbjct: 61  DEKSSSTPPIV---KDMHGSNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEG 120

Query: 123 VHEISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLTIS 182
           + E SS EP SKL+STKTS PRMSWADMTQEDELE EEDECESEKR+VNVN STGKLTIS
Sbjct: 121 LPETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEVEEDECESEKRVVNVNASTGKLTIS 180

Query: 183 KVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV 242
           K+  KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV
Sbjct: 181 KI--KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV 240

Query: 243 DHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIV 302
           DHVYALQEMG+KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPG+LR+EIV
Sbjct: 241 DHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIV 300

Query: 303 DPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLS 362
           DPIPSLFKVIIRR+VRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLS
Sbjct: 301 DPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLS 360

Query: 363 ECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITFRR 422
           ECNI+FG+NLSIVGPGEFSGP+AIPLPVGSVLVLNGNGADVAKHCVPAVP KRISITFRR
Sbjct: 361 ECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRR 420

Query: 423 IDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMRTRGSGN 482
           ID+SKRP EYASEPDLQGIQPLPYE SENDVPTSP+SSERE+RRQPF RGS MRTRGSGN
Sbjct: 421 IDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGN 480

Query: 483 RSDNRFEPRNPGRAQHRSAD-RRSRGNLDS 512
           RSD RFEPRN GRAQHR AD RRSR NLDS
Sbjct: 481 RSDTRFEPRNSGRAQHRPADNRRSRVNLDS 505

BLAST of Lag0013487 vs. ExPASy TrEMBL
Match: A0A6J1IMQ3 (RNA demethylase ALKBH5 OS=Cucurbita maxima OX=3661 GN=LOC111478802 PE=4 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 1.5e-257
Identity = 453/508 (89.17%), Postives = 476/508 (93.70%), Query Frame = 0

Query: 1   MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAV 60
           M ISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCR+CT+LLSDRIRALDPA 
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDPAS 60

Query: 61  RPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120
           R +ENSSS PP  V    MHESNGNQ+DACDANSLGSWKDEAETNSLGSWKDGINEGNEA
Sbjct: 61  RTDENSSSTPPVEV----MHESNGNQNDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120

Query: 121 DGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEE--DECESEKRLVNVNTSTGK 180
           DGV E SS  PS+K+ S KTSGPRMSWADMTQEDELEEEE  DE ESEKR+V+VN ST K
Sbjct: 121 DGVPETSSSVPSTKVVSIKTSGPRMSWADMTQEDELEEEEEDDEYESEKRVVSVNESTRK 180

Query: 181 LTISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQ 240
           LTISKVIEKPKLSREQRE+IRF+NVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQ
Sbjct: 181 LTISKVIEKPKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQ 240

Query: 241 KRIVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILR 300
           KRIVDHVY LQEMG+KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILR
Sbjct: 241 KRIVDHVYLLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILR 300

Query: 301 SEIVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTV 360
           SEIVDPIPSLFKVIIRRLVRWHVLPPTCVP+SCIVNIY+EGDCIPPHID+HDFVRPFCTV
Sbjct: 301 SEIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYDEGDCIPPHIDSHDFVRPFCTV 360

Query: 361 SFLSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISI 420
           SFLSECNI+FGTNLSIVGPGEFSGP+AIPLPVGSVLVLNGNGADVAKHCVPAVP KRISI
Sbjct: 361 SFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISI 420

Query: 421 TFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMRTR 480
           TFRR+DESK PIEYA EPDLQGIQPLPYEASEN+VPTSP+SSERE+RRQ FRRGS MRTR
Sbjct: 421 TFRRMDESKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQSFRRGSHMRTR 480

Query: 481 GSGNRSDNRFEPRNPGRAQHRSADRRSR 507
           GSGNR+D RF+ RN GR +H SADRRSR
Sbjct: 481 GSGNRTDTRFDSRNLGRGRHSSADRRSR 504

BLAST of Lag0013487 vs. ExPASy TrEMBL
Match: A0A6J1GFD0 (uncharacterized protein LOC111453671 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453671 PE=4 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 2.8e-256
Identity = 451/507 (88.95%), Postives = 474/507 (93.49%), Query Frame = 0

Query: 1   MPISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAV 60
           M ISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCR+CT+LLSDRIRALDPA 
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDPAA 60

Query: 61  RPNENSSSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGINEGNEA 120
           R +ENSSS PP  V    MHESNGNQ+DACDANSLGSWKDEAETNSL SWKDGINEGNEA
Sbjct: 61  RTDENSSSTPPVEV----MHESNGNQNDACDANSLGSWKDEAETNSLESWKDGINEGNEA 120

Query: 121 DGVHEISSCEPSSKLNSTKTSGPRMSWADMTQEDEL-EEEEDECESEKRLVNVNTSTGKL 180
           DGV E SS   SSK+ STKTSGPRMSWADMTQEDEL EEEEDE ESEKR+V+VN ST KL
Sbjct: 121 DGVPETSSSVQSSKVVSTKTSGPRMSWADMTQEDELEEEEEDEYESEKRVVSVNESTRKL 180

Query: 181 TISKVIEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQK 240
           TISKVIEK KLSREQRE+IRF+NVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQK
Sbjct: 181 TISKVIEKAKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQK 240

Query: 241 RIVDHVYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRS 300
           RIVDHVY+LQEMG+KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGI+RS
Sbjct: 241 RIVDHVYSLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGIIRS 300

Query: 301 EIVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVS 360
           EIVDPIPSLFKVIIRRLVRWHVLPPTCVP+SCIVNIY EGDCIPPHID+HDFVRPFCTVS
Sbjct: 301 EIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYNEGDCIPPHIDSHDFVRPFCTVS 360

Query: 361 FLSECNILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISIT 420
           FLSECNI+FGTNLSIVGPGEFSGP+AIPLPVGSVLVLNGNGADVAKHCVPAVP KRISIT
Sbjct: 361 FLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISIT 420

Query: 421 FRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMRTRG 480
           FRR+D SK PIEYA EPDLQGIQPLPYEASEN+VPTSP+SSERE+RRQPFRRGS MRTRG
Sbjct: 421 FRRMDGSKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQPFRRGSHMRTRG 480

Query: 481 SGNRSDNRFEPRNPGRAQHRSADRRSR 507
           SGNR+D RF+ RN GR +H SADRRSR
Sbjct: 481 SGNRTDTRFDSRNSGRGRHSSADRRSR 503

BLAST of Lag0013487 vs. TAIR 10
Match: AT2G17970.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.3e-157
Identity = 295/518 (56.95%), Postives = 360/518 (69.50%), Query Frame = 0

Query: 12  DPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAVRPNENS----- 71
           DPFL  Y+PSEL+IASEFLT WLPFLS+DLC+DC  LLS+RIR+LDPA   N        
Sbjct: 4   DPFLRQYQPSELKIASEFLTNWLPFLSKDLCKDCNHLLSNRIRSLDPAHCSNNTDKVDGE 63

Query: 72  -SSAPPPIVEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDG-INEGNEADGVH 131
             +    +VE+     ++ N DD  D  S     ++ + +SLGSWK   I  G+  +   
Sbjct: 64  CKTGSCSVVENMGSERASNNVDDNYDEKSENG--EDCDNHSLGSWKGSEIVFGSFPEDFS 123

Query: 132 EISSCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLTISKV 191
            +    P+      +T+ PRM WADM QEDE +EEE+E E E+           +   K 
Sbjct: 124 SVLQSRPA----VVETASPRMRWADMAQEDEFDEEEEEEEEERDSSRKGFDASSM---KT 183

Query: 192 IEKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDH 251
            EKPKLSR+QRE +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD 
Sbjct: 184 PEKPKLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQ 243

Query: 252 VYALQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIVDP 311
           VY LQE G++GEL++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPGIL+ E VDP
Sbjct: 244 VYQLQEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDP 303

Query: 312 IPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSEC 371
           +P LFKVIIR+L++WHVLPPTCVPDSCIVNIY+EGDCIPPHIDNHDF+RPFCT+SFLSEC
Sbjct: 304 LPHLFKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSEC 363

Query: 372 NILFGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITFRRID 431
           +ILFG+NL + GPG+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVP KRISITFR++D
Sbjct: 364 DILFGSNLKVEGPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMD 423

Query: 432 ESKRPIEYASEPDLQGIQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMR---TRGSG 491
           ESKRP+ +  EPDLQGI+PLP + + +        S     R     G+  R    RG G
Sbjct: 424 ESKRPVWFTPEPDLQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGG 483

Query: 492 NRSDNR--FEPRNPGR---------AQHRSADRRSRGN 509
           N  D+R  + P              +Q R   R SR N
Sbjct: 484 NGYDSRGYYNPERSSEHNDSGDWPSSQRRGMPRPSRRN 505

BLAST of Lag0013487 vs. TAIR 10
Match: AT4G36090.3 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 528.1 bits (1359), Expect = 7.9e-150
Identity = 289/498 (58.03%), Postives = 351/498 (70.48%), Query Frame = 0

Query: 11  EDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTQLLSDRIRALDPAVRPNENSSSAP 70
           ED FL  Y+ SEL+IASEFLT WLPFLSRDLC DC  +LSDRIR+LDP    N    +  
Sbjct: 7   EDVFLAKYQSSELKIASEFLTNWLPFLSRDLCNDCAHVLSDRIRSLDPEHCSNGEVKAGS 66

Query: 71  PPIVEDQDMHESNGNQDDACDANSLGSWKDE--AETNSLGSWKDG-INEGNEADGVHEIS 130
             +V++ +  E +  + +  D N + +  D     ++SL S K G    G+ +D V   S
Sbjct: 67  GSMVDNMESEEVDNMESEEVD-NKMDNIYDGKLCVSHSLDSLKAGAFVFGSLSDSV---S 126

Query: 131 SCEPSSKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLTISKVIEK 190
              PSS+   ++ +  +MSWADM +ED L EEED+ E+E     V+ S       K  EK
Sbjct: 127 HAMPSSQSTVSEAASAQMSWADMGEEDGL-EEEDQKENELGSHGVDVSPSVGDSMKTPEK 186

Query: 191 PKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYA 250
            KLSRE+RE  RFMNV + K F C E+ +G+ VNILEGLELHTG+FSA EQK+IVD VY 
Sbjct: 187 RKLSREERERYRFMNVKKMKVFSCYEKVRGRSVNILEGLELHTGVFSAVEQKKIVDFVYE 246

Query: 251 LQEMGQKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIVDPIPS 310
           LQE G++GELRERTF+AP KWM+GKGRVT+QFGCCYNYAPDK GNPPGIL+   VDP+PS
Sbjct: 247 LQEKGRRGELRERTFTAPHKWMRGKGRVTIQFGCCYNYAPDKAGNPPGILQRGDVDPMPS 306

Query: 311 LFKVIIRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIL 370
           +FKVII+RLV WHVLPPTCVPDSCIVNIYEE DCIPPHIDNHDF+RPFCTVSFLSECNIL
Sbjct: 307 IFKVIIKRLVGWHVLPPTCVPDSCIVNIYEEDDCIPPHIDNHDFLRPFCTVSFLSECNIL 366

Query: 371 FGTNLSIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITFRRIDESK 430
           FG+NL ++GPGEFSG  +IPLPVGSVLVL GNGADVAKHCVPAVP KRISITFR++DESK
Sbjct: 367 FGSNLKVLGPGEFSGSYSIPLPVGSVLVLKGNGADVAKHCVPAVPTKRISITFRKMDESK 426

Query: 431 RPIEYASEPDLQGIQPLPYEASENDVPT-SPISSEREMRRQPFRRGSQMRTRGSGNRSDN 490
           RP+ +  EPDL+ I+PLPYE +    P  + ISS R    Q     +     G G++   
Sbjct: 427 RPVGFTPEPDLEEIKPLPYEHTTPSTPADAVISSSRSSIDQNGSNHNNRTAHGGGSKYRR 486

Query: 491 RFEPRNPGRAQHRSADRR 505
             +  +  R +  S  RR
Sbjct: 487 SRDYHSESRERSSSGQRR 499

BLAST of Lag0013487 vs. TAIR 10
Match: AT2G17970.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.5e-135
Identity = 255/442 (57.69%), Postives = 311/442 (70.36%), Query Frame = 0

Query: 82  SNGNQDDACDANSLGSWKDEAETNSLGSWKDG-INEGNEADGVHEISSCEPSSKLNSTKT 141
           ++ N DD  D  S     ++ + +SLGSWK   I  G+  +    +    P+      +T
Sbjct: 6   ASNNVDDNYDEKSENG--EDCDNHSLGSWKGSEIVFGSFPEDFSSVLQSRPA----VVET 65

Query: 142 SGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLTISKVIEKPKLSREQREYIRF 201
           + PRM WADM QEDE +EEE+E E E+           +   K  EKPKLSR+QRE +R 
Sbjct: 66  ASPRMRWADMAQEDEFDEEEEEEEEERDSSRKGFDASSM---KTPEKPKLSRDQRENLRL 125

Query: 202 MNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGQKGELRER 261
           +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD VY LQE G++GEL++R
Sbjct: 126 INVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQVYQLQEKGRRGELKKR 185

Query: 262 TFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIVDPIPSLFKVIIRRLVRWH 321
           TF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPGIL+ E VDP+P LFKVIIR+L++WH
Sbjct: 186 TFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDPLPHLFKVIIRKLIKWH 245

Query: 322 VLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNILFGTNLSIVGPGEF 381
           VLPPTCVPDSCIVNIY+EGDCIPPHIDNHDF+RPFCT+SFLSEC+ILFG+NL + GPG+F
Sbjct: 246 VLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILFGSNLKVEGPGDF 305

Query: 382 SGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITFRRIDESKRPIEYASEPDLQG 441
           SG  +IPLPVGSVLVLNGNGADVAKHCVPAVP KRISITFR++DESKRP+ +  EPDLQG
Sbjct: 306 SGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKRPVWFTPEPDLQG 365

Query: 442 IQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMR---TRGSGNRSDNR--FEPRNPGR 501
           I+PLP + + +        S     R     G+  R    RG GN  D+R  + P     
Sbjct: 366 IEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGGNGYDSRGYYNPERSSE 425

Query: 502 ---------AQHRSADRRSRGN 509
                    +Q R   R SR N
Sbjct: 426 HNDSGDWPSSQRRGMPRPSRRN 431

BLAST of Lag0013487 vs. TAIR 10
Match: AT2G17970.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.5e-135
Identity = 255/442 (57.69%), Postives = 311/442 (70.36%), Query Frame = 0

Query: 82  SNGNQDDACDANSLGSWKDEAETNSLGSWKDG-INEGNEADGVHEISSCEPSSKLNSTKT 141
           ++ N DD  D  S     ++ + +SLGSWK   I  G+  +    +    P+      +T
Sbjct: 6   ASNNVDDNYDEKSENG--EDCDNHSLGSWKGSEIVFGSFPEDFSSVLQSRPA----VVET 65

Query: 142 SGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLTISKVIEKPKLSREQREYIRF 201
           + PRM WADM QEDE +EEE+E E E+           +   K  EKPKLSR+QRE +R 
Sbjct: 66  ASPRMRWADMAQEDEFDEEEEEEEEERDSSRKGFDASSM---KTPEKPKLSRDQRENLRL 125

Query: 202 MNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGQKGELRER 261
           +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD VY LQE G++GEL++R
Sbjct: 126 INVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQVYQLQEKGRRGELKKR 185

Query: 262 TFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIVDPIPSLFKVIIRRLVRWH 321
           TF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPGIL+ E VDP+P LFKVIIR+L++WH
Sbjct: 186 TFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDPLPHLFKVIIRKLIKWH 245

Query: 322 VLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNILFGTNLSIVGPGEF 381
           VLPPTCVPDSCIVNIY+EGDCIPPHIDNHDF+RPFCT+SFLSEC+ILFG+NL + GPG+F
Sbjct: 246 VLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILFGSNLKVEGPGDF 305

Query: 382 SGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITFRRIDESKRPIEYASEPDLQG 441
           SG  +IPLPVGSVLVLNGNGADVAKHCVPAVP KRISITFR++DESKRP+ +  EPDLQG
Sbjct: 306 SGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKRPVWFTPEPDLQG 365

Query: 442 IQPLPYEASENDVPTSPISSEREMRRQPFRRGSQMR---TRGSGNRSDNR--FEPRNPGR 501
           I+PLP + + +        S     R     G+  R    RG GN  D+R  + P     
Sbjct: 366 IEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGGNGYDSRGYYNPERSSE 425

Query: 502 ---------AQHRSADRRSRGN 509
                    +Q R   R SR N
Sbjct: 426 HNDSGDWPSSQRRGMPRPSRRN 431

BLAST of Lag0013487 vs. TAIR 10
Match: AT4G36090.2 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 459.5 bits (1181), Expect = 3.4e-129
Identity = 252/433 (58.20%), Postives = 304/433 (70.21%), Query Frame = 0

Query: 74  VEDQDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDG-INEGNEADGVHEISSCEPS 133
           V++ +  E +   D+  D     S       +SL S K G    G+ +D V   S   PS
Sbjct: 10  VDNMESEEVDNKMDNIYDGKLCVS-------HSLDSLKAGAFVFGSLSDSV---SHAMPS 69

Query: 134 SKLNSTKTSGPRMSWADMTQEDELEEEEDECESEKRLVNVNTSTGKLTISKVIEKPKLSR 193
           S+   ++ +  +MSWADM +ED L EEED+ E+E     V+ S       K  EK KLSR
Sbjct: 70  SQSTVSEAASAQMSWADMGEEDGL-EEEDQKENELGSHGVDVSPSVGDSMKTPEKRKLSR 129

Query: 194 EQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQEMG 253
           E+RE  RFMNV + K F C E+ +G+ VNILEGLELHTG+FSA EQK+IVD VY LQE G
Sbjct: 130 EERERYRFMNVKKMKVFSCYEKVRGRSVNILEGLELHTGVFSAVEQKKIVDFVYELQEKG 189

Query: 254 QKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIVDPIPSLFKVI 313
           ++GELRERTF+AP KWM+GKGRVT+QFGCCYNYAPDK GNPPGIL+   VDP+PS+FKVI
Sbjct: 190 RRGELRERTFTAPHKWMRGKGRVTIQFGCCYNYAPDKAGNPPGILQRGDVDPMPSIFKVI 249

Query: 314 IRRLVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNILFGTNL 373
           I+RLV WHVLPPTCVPDSCIVNIYEE DCIPPHIDNHDF+RPFCTVSFLSECNILFG+NL
Sbjct: 250 IKRLVGWHVLPPTCVPDSCIVNIYEEDDCIPPHIDNHDFLRPFCTVSFLSECNILFGSNL 309

Query: 374 SIVGPGEFSGPMAIPLPVGSVLVLNGNGADVAKHCVPAVPMKRISITFRRIDESKRPIEY 433
            ++GPGEFSG  +IPLPVGSVLVL GNGADVAKHCVPAVP KRISITFR++DESKRP+ +
Sbjct: 310 KVLGPGEFSGSYSIPLPVGSVLVLKGNGADVAKHCVPAVPTKRISITFRKMDESKRPVGF 369

Query: 434 ASEPDLQGIQPLPYEASENDVPT-SPISSEREMRRQPFRRGSQMRTRGSGNRSDNRFEPR 493
             EPDL+ I+PLPYE +    P  + ISS R    Q     +     G G++     +  
Sbjct: 370 TPEPDLEEIKPLPYEHTTPSTPADAVISSSRSSIDQNGSNHNNRTAHGGGSKYRRSRDYH 429

Query: 494 NPGRAQHRSADRR 505
           +  R +  S  RR
Sbjct: 430 SESRERSSSGQRR 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882153.18.2e-26691.30RNA demethylase ALKBH9B [Benincasa hispida][more]
XP_022926828.12.5e-26289.63uncharacterized protein LOC111433825 [Cucurbita moschata][more]
XP_023518278.17.2e-26289.24uncharacterized protein LOC111781804 [Cucurbita pepo subsp. pepo][more]
KAG6594779.13.0e-26088.85RNA demethylase ALKBH9B, partial [Cucurbita argyrosperma subsp. sororia] >KAG702... [more]
XP_023544551.16.7e-26089.74uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo] >XP_023544552.... [more]
Match NameE-valueIdentityDescription
Q9SL491.9e-15656.95RNA demethylase ALKBH9B OS=Arabidopsis thaliana OX=3702 GN=ALKBH9B PE=1 SV=1[more]
Q9ZT921.5e-2834.25RNA demethylase ALKBH10B OS=Arabidopsis thaliana OX=3702 GN=ALKBH10B PE=1 SV=1[more]
Q66JG88.8e-1332.21RNA demethylase ALKBH5 OS=Xenopus tropicalis OX=8364 GN=alkbh5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EJB31.2e-26289.63uncharacterized protein LOC111433825 OS=Cucurbita moschata OX=3662 GN=LOC1114338... [more]
A0A6J1KUK55.5e-26088.85uncharacterized protein LOC111497336 OS=Cucurbita maxima OX=3661 GN=LOC111497336... [more]
A0A6J1BY698.0e-25988.82uncharacterized protein LOC111005802 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1IMQ31.5e-25789.17RNA demethylase ALKBH5 OS=Cucurbita maxima OX=3661 GN=LOC111478802 PE=4 SV=1[more]
A0A6J1GFD02.8e-25688.95uncharacterized protein LOC111453671 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT2G17970.11.3e-15756.952-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G36090.37.9e-15058.03oxidoreductase, 2OG-Fe(II) oxygenase family protein [more]
AT2G17970.22.5e-13557.692-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G17970.32.5e-13557.692-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G36090.23.4e-12958.20oxidoreductase, 2OG-Fe(II) oxygenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 153..173
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 86..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..145
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..145
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 482..511
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 431..511
NoneNo IPR availablePANTHERPTHR31447:SF5RNA DEMETHYLASE ALKBH9Bcoord: 5..505
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 222..423
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likePFAMPF135322OG-FeII_Oxy_2coord: 226..421
e-value: 7.8E-14
score: 52.3
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3D2.60.120.590coord: 210..431
e-value: 1.2E-73
score: 249.4
IPR044842RNA demethylase ALKBH9B/ALKBH10B-likePANTHERPTHR31447HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN-RELATEDcoord: 5..505
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 327..424
score: 10.422147

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013487.1Lag0013487.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070988 demethylation
biological_process GO:0032259 methylation
biological_process GO:0006402 mRNA catabolic process
molecular_function GO:0032451 demethylase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003729 mRNA binding