Lag0013437 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013437
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionheavy metal-associated isoprenylated plant protein 3-like
Locationchr1: 50296116 .. 50296603 (+)
RNA-Seq ExpressionLag0013437
SyntenyLag0013437
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCAGACCAAAACTGTCTTGAAAATTGACATCGCTTGCCAAAAATGCAAGACCAAAGTCCTCAAAGCTGTTACTAGTATAGAAGGTACCTTCTTCTTCTTCTTCTTTGATATTAATTAATTTGGATTATTCCATCGTTAATTGAATTATTGCATGCAATTAATTTATGTGTAGGCGTGGACAAGGTTGAGGCGGACGAAGCGAAGGGGACGTTGGCCGTGACGGGGACGGCGGATCCCTTCGACATAGTTACACGTACGAGGAAGGCGATTTCTTGCGCCGGCAAGATTGCGGATGTGGTCAGCATTGGACCGCCGCCCAAACCGGACGACAAGAAGTCCGATGACAAGAAGTCCGGCGACAAGAAGACCGACGACAAGAAGCCCGACCCGCCGCCTTTCTGCCCATGTCCACCATACCCTCCTTATGGGTCTTCGTACATCGTCGTGCCTCATGAAACTCCACCTCCTTGCTCTATTCTTTAG

mRNA sequence

ATGGCGCAGACCAAAACTGTCTTGAAAATTGACATCGCTTGCCAAAAATGCAAGACCAAAGTCCTCAAAGCTGTTACTAGTATAGAAGGCGTGGACAAGGTTGAGGCGGACGAAGCGAAGGGGACGTTGGCCGTGACGGGGACGGCGGATCCCTTCGACATAGTTACACGTACGAGGAAGGCGATTTCTTGCGCCGGCAAGATTGCGGATGTGGTCAGCATTGGACCGCCGCCCAAACCGGACGACAAGAAGTCCGATGACAAGAAGTCCGGCGACAAGAAGACCGACGACAAGAAGCCCGACCCGCCGCCTTTCTGCCCATGTCCACCATACCCTCCTTATGGGTCTTCGTACATCGTCGTGCCTCATGAAACTCCACCTCCTTGCTCTATTCTTTAG

Coding sequence (CDS)

ATGGCGCAGACCAAAACTGTCTTGAAAATTGACATCGCTTGCCAAAAATGCAAGACCAAAGTCCTCAAAGCTGTTACTAGTATAGAAGGCGTGGACAAGGTTGAGGCGGACGAAGCGAAGGGGACGTTGGCCGTGACGGGGACGGCGGATCCCTTCGACATAGTTACACGTACGAGGAAGGCGATTTCTTGCGCCGGCAAGATTGCGGATGTGGTCAGCATTGGACCGCCGCCCAAACCGGACGACAAGAAGTCCGATGACAAGAAGTCCGGCGACAAGAAGACCGACGACAAGAAGCCCGACCCGCCGCCTTTCTGCCCATGTCCACCATACCCTCCTTATGGGTCTTCGTACATCGTCGTGCCTCATGAAACTCCACCTCCTTGCTCTATTCTTTAG

Protein sequence

MAQTKTVLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRKAISCAGKIADVVSIGPPPKPDDKKSDDKKSGDKKTDDKKPDPPPFCPCPPYPPYGSSYIVVPHETPPPCSIL
Homology
BLAST of Lag0013437 vs. NCBI nr
Match: XP_008440757.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo])

HSP 1 Score: 207.2 bits (526), Expect = 8.5e-50
Identity = 112/136 (82.35%), Postives = 121/136 (88.97%), Query Frame = 0

Query: 1   MAQTKTVLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRK 60
           MAQTKTVLK+DIACQKCKTKVLKAVT+IEGVDKVE DEAKGTLAVTGTADPF+IV RTRK
Sbjct: 1   MAQTKTVLKLDIACQKCKTKVLKAVTAIEGVDKVETDEAKGTLAVTGTADPFEIVKRTRK 60

Query: 61  AISCAGKIADVVSIGPPPKPDDKKSDDKKSGDKK-TDDKKPDPPPFCPCPPY---PPYGS 120
           AI+CAGKIADVVSIGPPPKP +KK D+KK GDKK  D KKP+PPP CPCPPY   P YGS
Sbjct: 61  AIACAGKIADVVSIGPPPKPGEKKPDEKKEGDKKPADPKKPEPPP-CPCPPYHCPPYYGS 120

Query: 121 SYIVVPHETPPPCSIL 133
           SY++VP ET PPCSIL
Sbjct: 121 SYVIVPRETYPPCSIL 135

BLAST of Lag0013437 vs. NCBI nr
Match: KGN48859.1 (hypothetical protein Csa_003365 [Cucumis sativus])

HSP 1 Score: 202.6 bits (514), Expect = 2.1e-48
Identity = 107/135 (79.26%), Postives = 119/135 (88.15%), Query Frame = 0

Query: 1   MAQTKTVLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRK 60
           MA TKTV+K+DIACQKCKTKVLKAVT+IEGVDKVE DEAKGTLAV GTADPF+IV RTRK
Sbjct: 1   MAVTKTVMKLDIACQKCKTKVLKAVTAIEGVDKVETDEAKGTLAVIGTADPFEIVKRTRK 60

Query: 61  AISCAGKIADVVSIGPPPKPDDKKSDDKKSGDKKTDDKKPDPPPFCPCPPY---PPYGSS 120
           AI+CAGK+ADVVSIGPPPKPD+KK ++KK  +KK  DKKPDPPP CPCPPY   P YGSS
Sbjct: 61  AIACAGKVADVVSIGPPPKPDEKKPEEKKQEEKKPVDKKPDPPP-CPCPPYPCPPYYGSS 120

Query: 121 YIVVPHETPPPCSIL 133
           Y++VPHET P CSIL
Sbjct: 121 YVIVPHETYPSCSIL 134

BLAST of Lag0013437 vs. NCBI nr
Match: XP_004149991.2 (uncharacterized protein LOC101205995 [Cucumis sativus])

HSP 1 Score: 202.6 bits (514), Expect = 2.1e-48
Identity = 107/135 (79.26%), Postives = 119/135 (88.15%), Query Frame = 0

Query: 1   MAQTKTVLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRK 60
           MA TKTV+K+DIACQKCKTKVLKAVT+IEGVDKVE DEAKGTLAV GTADPF+IV RTRK
Sbjct: 1   MAVTKTVMKLDIACQKCKTKVLKAVTAIEGVDKVETDEAKGTLAVIGTADPFEIVKRTRK 60

Query: 61  AISCAGKIADVVSIGPPPKPDDKKSDDKKSGDKKTDDKKPDPPPFCPCPPY---PPYGSS 120
           AI+CAGK+ADVVSIGPPPKPD+KK ++KK  +KK  DKKPDPPP CPCPPY   P YGSS
Sbjct: 61  AIACAGKVADVVSIGPPPKPDEKKPEEKKQEEKKPVDKKPDPPP-CPCPPYPCPPYYGSS 120

Query: 121 YIVVPHETPPPCSIL 133
           Y++VPHET P CSIL
Sbjct: 121 YVIVPHETYPSCSIL 134

BLAST of Lag0013437 vs. NCBI nr
Match: XP_038877711.1 (heavy metal-associated isoprenylated plant protein 43-like [Benincasa hispida])

HSP 1 Score: 201.4 bits (511), Expect = 4.7e-48
Identity = 109/133 (81.95%), Postives = 114/133 (85.71%), Query Frame = 0

Query: 1   MAQTKTVLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRK 60
           MAQTKTVLK+DIACQKCKTKVLKAVTSIEGVDKVE DEAKGTL+VTGTADP DIV RTRK
Sbjct: 1   MAQTKTVLKLDIACQKCKTKVLKAVTSIEGVDKVETDEAKGTLSVTGTADPVDIVKRTRK 60

Query: 61  AISCAGKIADVVSIGPPPKPDDKKSDDKKSGDKKTDDKKPDPPPF-CPCPPYPPYGSSYI 120
           AISCAGKI DVVSIGPPPKP++KK D     DKK DDKKP PPP  CPCPPYPPY  SY+
Sbjct: 61  AISCAGKIVDVVSIGPPPKPEEKKPD-----DKKPDDKKPAPPPCPCPCPPYPPYSGSYV 120

Query: 121 VVPHETPPPCSIL 133
           VVP ET P CSIL
Sbjct: 121 VVPCETNPSCSIL 128

BLAST of Lag0013437 vs. NCBI nr
Match: KAG7034127.1 (Heavy metal-associated isoprenylated plant protein 43, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 179.9 bits (455), Expect = 1.5e-41
Identity = 96/133 (72.18%), Postives = 109/133 (81.95%), Query Frame = 0

Query: 1   MAQTKTVLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRK 60
           MA TKT+LK+DIAC KCKTKVLKAVT++EGVDK+E DEAKGTLAVTGTADP+DIVTRTRK
Sbjct: 1   MAMTKTILKLDIACHKCKTKVLKAVTALEGVDKIEMDEAKGTLAVTGTADPYDIVTRTRK 60

Query: 61  AISCAGKIADVVSIGPPPKPDDKKSDDKKSGDKKTDDKKPDPPPFCPCPPYPP-YGSSYI 120
           AISCAGKIAD+ +IGPPPKP    +   K   + +    P PPP+CPCP YPP YGSSY+
Sbjct: 61  AISCAGKIADIDTIGPPPKP----NPPPKPTPQPSCSCPPCPPPYCPCPSYPPYYGSSYV 120

Query: 121 VVPHETPPPCSIL 133
           VVPHET P CSIL
Sbjct: 121 VVPHETYPSCSIL 129

BLAST of Lag0013437 vs. ExPASy Swiss-Prot
Match: Q9SFF7 (Heavy metal-associated isoprenylated plant protein 43 OS=Arabidopsis thaliana OX=3702 GN=HIPP43 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-10
Identity = 52/139 (37.41%), Postives = 73/139 (52.52%), Query Frame = 0

Query: 1   MAQTKTVLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRK 60
           M   K  +K+DI C KC + +++AVT IEGV+ +  DE    L V GT DP  + TR +K
Sbjct: 1   MTVKKVEIKVDINCGKCNSAIMEAVTEIEGVNHISLDEGNSILTVVGTMDPVCVATRLKK 60

Query: 61  AISCAGKIADVVSIGPPPKPDDKKSDDKKSGDKKTDDKKPDPPPFCPCPP----YPPYGS 120
                 +   ++S+GPPPKP +          K  + +KP PPP  P PP     PPY +
Sbjct: 61  ----IKQKPVIISVGPPPKPPEP--------PKPPEPEKPKPPP-APEPPKHVCKPPYCN 120

Query: 121 SYIVV---PHETPPPCSIL 133
           S  VV    +E+   C+IL
Sbjct: 121 SCDVVSVTTYESGSGCTIL 126

BLAST of Lag0013437 vs. ExPASy Swiss-Prot
Match: Q8GWS3 (Heavy metal-associated isoprenylated plant protein 2 OS=Arabidopsis thaliana OX=3702 GN=HIPP02 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.4e-09
Identity = 36/88 (40.91%), Postives = 50/88 (56.82%), Query Frame = 0

Query: 1  MAQTKTVLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRK 60
          M   K  +K+DI C+KCK  +++AVT +EGV+ V  D+ K  L V GT DP  +  + +K
Sbjct: 1  MTTKKIEIKVDIDCEKCKHAIMEAVTELEGVNIVSLDQEKSILTVVGTMDPVCVAEQLKK 60

Query: 61 AISCAGKIADVVSIGPPPKPDDKKSDDK 89
                K   V+S+GPP KPD K    K
Sbjct: 61 ----INKKPVVISVGPPKKPDPKPDPKK 84

BLAST of Lag0013437 vs. ExPASy Swiss-Prot
Match: O03982 (Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX=3702 GN=HIPP39 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 7.9e-06
Identity = 34/100 (34.00%), Postives = 57/100 (57.00%), Query Frame = 0

Query: 5   KTVLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRKAISC 64
           K VLK+D+   + K K LK V+++ G+D +  D  +  L V GT DP ++V++ RK    
Sbjct: 3   KIVLKLDLHDDRAKQKALKTVSTLPGIDSIAMDMKEKKLTVIGTVDPVNVVSKLRK---- 62

Query: 65  AGKIADVVSIGPPPKPD-DKKSDDKKSGDKKTDDKKPDPP 104
              + D+V +GP  +P+ +KK + KK G  +   K+ + P
Sbjct: 63  YWPMTDIVLVGPAKEPEKEKKEEPKKEGGGEPPKKEGEAP 98

BLAST of Lag0013437 vs. ExPASy Swiss-Prot
Match: Q9LTE3 (Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX=3702 GN=HIPP12 PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.0e-05
Identity = 43/123 (34.96%), Postives = 63/123 (51.22%), Query Frame = 0

Query: 7   VLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRKAISCAG 66
           VLK+D+ C+K K K +  V  + GV+ VE  +  G L VTG  D + IV + +K      
Sbjct: 5   VLKLDVHCEKTKQKAMSTVCCLSGVNSVEVKD--GKLTVTGEIDAYMIVKKLKKICH--- 64

Query: 67  KIADVVSIGPPPKPDDKKSDDKKSGDKKTDDKKPDPPPFCPCPPYPPYGSSYIVVPHETP 126
              + +S+GP  +P+ KK DD     KK + K PD   +   PP PPY  ++    +E P
Sbjct: 65  --TEFISVGPVKEPEKKKPDD----PKKPETKPPD-VIYHYVPPCPPYYHNFNGCYNEDP 115

Query: 127 PPC 130
             C
Sbjct: 125 NAC 115

BLAST of Lag0013437 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.9e-05
Identity = 41/108 (37.96%), Postives = 58/108 (53.70%), Query Frame = 0

Query: 7   VLKIDIACQKCKTKVLKAVTSIEGVDKVEADEAKGTLAVTGTADPFDIVTRTRKAISCAG 66
           VLK+D+ C+ C ++++K V S +GV+ V+++ A G L VTG  DP   V    K      
Sbjct: 29  VLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGALDP---VKLREKLEEKTK 88

Query: 67  KIADVVSIGPPPKPDDKK-------SDDKKSGDKK---TDDKKPDPPP 105
           K  D+VS  P PK + +K        D KKS +KK    +DKKP   P
Sbjct: 89  KKVDLVS--PQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKETP 131

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008440757.18.5e-5082.35PREDICTED: heavy metal-associated isoprenylated plant protein 3-like [Cucumis me... [more]
KGN48859.12.1e-4879.26hypothetical protein Csa_003365 [Cucumis sativus][more]
XP_004149991.22.1e-4879.26uncharacterized protein LOC101205995 [Cucumis sativus][more]
XP_038877711.14.7e-4881.95heavy metal-associated isoprenylated plant protein 43-like [Benincasa hispida][more]
KAG7034127.11.5e-4172.18Heavy metal-associated isoprenylated plant protein 43, partial [Cucurbita argyro... [more]
Match NameE-valueIdentityDescription
Q9SFF72.4e-1037.41Heavy metal-associated isoprenylated plant protein 43 OS=Arabidopsis thaliana OX... [more]
Q8GWS33.4e-0940.91Heavy metal-associated isoprenylated plant protein 2 OS=Arabidopsis thaliana OX=... [more]
O039827.9e-0634.00Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX... [more]
Q9LTE31.0e-0534.96Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX... [more]
Q9FJH53.9e-0537.96Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 1..77
e-value: 9.1E-16
score: 59.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..108
NoneNo IPR availablePANTHERPTHR45811COPPER TRANSPORT PROTEIN FAMILY-RELATEDcoord: 4..131
NoneNo IPR availablePANTHERPTHR45811:SF13OS04G0661100 PROTEINcoord: 4..131
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 8..66
e-value: 4.4E-10
score: 39.8
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 12..71
score: 8.542343
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 3..60

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013437.1Lag0013437.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding