Lag0013417 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013417
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptiontransmembrane protein 53
Locationchr1: 50050282 .. 50052367 (-)
RNA-Seq ExpressionLag0013417
SyntenyLag0013417
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCTCCAGTAAGGGCATTTCGGCCCTACGTTCTTAATCGCCGCCTGTTTTCAAAAACAATTCCTCCATCGCGCCTCGTTGCATCTCCTGAATCTAATCCGAGCGCACTTTTCCGGCAATCTCCAACCTATTTGCATCCTCCTCCATCTCGAAACTTCCCCGGTTCCAAAATCTCACTCTCACTCAACCTTTCTCACTGCTCCTCCTCGCCCTTCGGATCTTCTTCCTCTTCTCTTGGCTTTAACCCTTCTAATTTTCTTTCTTCTTTACCCTCAATACAATCTTTCGGCTCCCAATTCGAACCTGATCACTTCAATTCGTTCCTATCCGATTCGAATGGCAGTACCTGGACTTGGAATCGAGCTTCAGAGAGTGCAATTAAGGGAAACGTGGGGGTTTTAGGAGGCGAAAAGGGTTCGGCGACCGTTGTGTTGTTAGGTTGGCTCGGGTCGAAGACAAAGCATCTGAGGAGGTACGTGGAGTGGTACAATGCAAGGGGTATCAACGCATTGACGTTTGTGGTTGATCCGACCGAGTTTCTTTGGTTTGCATTGAGTCGGAGAGTTGAGCAGCGGATTTCGGATTTGGCGGTCGAACTCATTTCTTGGTTATCAGACGAGGAAGAGAGTGATAAAGATCGGTCCCTGATATTTCACACTTTTAGCAATACAGGCTGGTTTGTGTAAGTTGAATTATGTTATCGGTATCTCCTTGGAGTTGTTTTGAGATTTCAACTAGAATTAGCGTTGGTTTCTTTTTCCCTTTTCAGATACGGCGCCATTCTTGAAATTTTGCAGGGTCGGAAGGATTTGTTGGAGAAGATCAAAGGATGCATTGTTGATTCTGGAGGAGGTGATCCATTGAATCCTCAGGTTTGTGAACTGATAGTTATATGATTTTGGAACTGCTGCACGACAATATACATATATATATATATGATCGTTCTTTGAACTGTTGGAAAAACAACATCTGCATAACAAATGGCGAGAATGGAAGGATTTGGTGGTGAGAATTTAGTAGGAACAGGTTATTGCTCTTCAAATATCATATGAATCTCAATCTCTATCTGTCCCTTTGATGCTCTTTCTTTTTCTCTCTGTTCCCTCTTTCTTTCTTTAGTTCCAAATTGTTTGAATCAGATTGTAATGACTGCAACAGGGAGTTTTCTTATTGATTACAATTATTTACCCAAGATCTCATTTAAAATTTCCAACTCCCAGTTTCCCAAGGCTTCTAAACAGTCCACAAAATTTGGTTAAAATTTTCTTGTCATAAACTATTGAAGCATATGCAAACAACTAGTTAGCTTATATCCAATCTGATTTAGATAGCATGCTTTTGTGTTATGTTAACATACATTGTTATCAATTTTTCTGCCTTCCTCGTCTGCTTTACAAATAAGATATCAACTACCATGCTTTCATCTAATCTGTTTTGCTTTCCACTTCATTCCCATTCAAAGTGGAAGAGAGAAATTTGATTTCATGGCTACTACGACACTGACAGGTTTGGGCAGCTGGATTTTCTGCAGCCATTCTCAAGAAAAACAGTTCTTCTACATCTCCTGTCGTTGATGGAGAGGAAATAGACAAGAAACCTCCTTGGATGGAAACCTTTGTTCTATCATCATTGGAGAAGTTTTTCTCAGTCGCTTTGAAGTTGCCAGACGTCGATAAGTGAGTTTTCTTATATCCAGACATGGATCCATATTCACTAGAAGTTAGAATGACCATATATTTATCGTATGGATGCATATCAATTGTTGAGAAATGAACATTTCCTTGCAGGAGATTGAAGAAGATTGTTTCAGTTCTTACAGAGAACCAGCCTTTTTATCCTGAGCTTTATCTGTACAGTTCAGGAGATAGAGTTGTGCCGTTCGAGTCAATCGAGCTACTTATCGAGAAGAGAAAGAAGACGGGAAGGAAGGTTTTGTCACACGACTTTCGCTCATCGCCGCATGTTGATCATTACAGGACATACCCAGATGTATACTCATCACAGCTTCACAAGTTCTTGGTAGAGTCTTTTTCTTCATAGGGTTGCTGTGGATTGGTGCATATGGCAGATAATTTGTGCTGA

mRNA sequence

ATGGAAGCTCCAGTAAGGGCATTTCGGCCCTACGTTCTTAATCGCCGCCTGTTTTCAAAAACAATTCCTCCATCGCGCCTCGTTGCATCTCCTGAATCTAATCCGAGCGCACTTTTCCGGCAATCTCCAACCTATTTGCATCCTCCTCCATCTCGAAACTTCCCCGGTTCCAAAATCTCACTCTCACTCAACCTTTCTCACTGCTCCTCCTCGCCCTTCGGATCTTCTTCCTCTTCTCTTGGCTTTAACCCTTCTAATTTTCTTTCTTCTTTACCCTCAATACAATCTTTCGGCTCCCAATTCGAACCTGATCACTTCAATTCGTTCCTATCCGATTCGAATGGCAGTACCTGGACTTGGAATCGAGCTTCAGAGAGTGCAATTAAGGGAAACGTGGGGGTTTTAGGAGGCGAAAAGGGTTCGGCGACCGTTGTGTTGTTAGGTTGGCTCGGGTCGAAGACAAAGCATCTGAGGAGGTACGTGGAGTGGTACAATGCAAGGGGTATCAACGCATTGACGTTTGTGGTTGATCCGACCGAGTTTCTTTGGTTTGCATTGAGTCGGAGAGTTGAGCAGCGGATTTCGGATTTGGCGGTCGAACTCATTTCTTGGTTATCAGACGAGGAAGAGAGTGATAAAGATCGGTCCCTGATATTTCACACTTTTAGCAATACAGGCTGGTTTGTATACGGCGCCATTCTTGAAATTTTGCAGGGTCGGAAGGATTTGTTGGAGAAGATCAAAGGATGCATTGTTGATTCTGGAGGAGGTGATCCATTGAATCCTCAGGTTTGGGCAGCTGGATTTTCTGCAGCCATTCTCAAGAAAAACAGTTCTTCTACATCTCCTGTCGTTGATGGAGAGGAAATAGACAAGAAACCTCCTTGGATGGAAACCTTTGTTCTATCATCATTGGAGAAGTTTTTCTCAGTCGCTTTGAAGTTGCCAGACGTCGATAAGAGATTGAAGAAGATTGTTTCAGTTCTTACAGAGAACCAGCCTTTTTATCCTGAGCTTTATCTGTACAGTTCAGGAGATAGAGTTGTGCCGTTCGAGTCAATCGAGCTACTTATCGAGAAGAGAAAGAAGACGGGAAGGAAGGTTTTGTCACACGACTTTCGCTCATCGCCGCATGTTGATCATTACAGGACATACCCAGATGTATACTCATCACAGCTTCACAAGTTCTTGGGTTGCTGTGGATTGGTGCATATGGCAGATAATTTGTGCTGA

Coding sequence (CDS)

ATGGAAGCTCCAGTAAGGGCATTTCGGCCCTACGTTCTTAATCGCCGCCTGTTTTCAAAAACAATTCCTCCATCGCGCCTCGTTGCATCTCCTGAATCTAATCCGAGCGCACTTTTCCGGCAATCTCCAACCTATTTGCATCCTCCTCCATCTCGAAACTTCCCCGGTTCCAAAATCTCACTCTCACTCAACCTTTCTCACTGCTCCTCCTCGCCCTTCGGATCTTCTTCCTCTTCTCTTGGCTTTAACCCTTCTAATTTTCTTTCTTCTTTACCCTCAATACAATCTTTCGGCTCCCAATTCGAACCTGATCACTTCAATTCGTTCCTATCCGATTCGAATGGCAGTACCTGGACTTGGAATCGAGCTTCAGAGAGTGCAATTAAGGGAAACGTGGGGGTTTTAGGAGGCGAAAAGGGTTCGGCGACCGTTGTGTTGTTAGGTTGGCTCGGGTCGAAGACAAAGCATCTGAGGAGGTACGTGGAGTGGTACAATGCAAGGGGTATCAACGCATTGACGTTTGTGGTTGATCCGACCGAGTTTCTTTGGTTTGCATTGAGTCGGAGAGTTGAGCAGCGGATTTCGGATTTGGCGGTCGAACTCATTTCTTGGTTATCAGACGAGGAAGAGAGTGATAAAGATCGGTCCCTGATATTTCACACTTTTAGCAATACAGGCTGGTTTGTATACGGCGCCATTCTTGAAATTTTGCAGGGTCGGAAGGATTTGTTGGAGAAGATCAAAGGATGCATTGTTGATTCTGGAGGAGGTGATCCATTGAATCCTCAGGTTTGGGCAGCTGGATTTTCTGCAGCCATTCTCAAGAAAAACAGTTCTTCTACATCTCCTGTCGTTGATGGAGAGGAAATAGACAAGAAACCTCCTTGGATGGAAACCTTTGTTCTATCATCATTGGAGAAGTTTTTCTCAGTCGCTTTGAAGTTGCCAGACGTCGATAAGAGATTGAAGAAGATTGTTTCAGTTCTTACAGAGAACCAGCCTTTTTATCCTGAGCTTTATCTGTACAGTTCAGGAGATAGAGTTGTGCCGTTCGAGTCAATCGAGCTACTTATCGAGAAGAGAAAGAAGACGGGAAGGAAGGTTTTGTCACACGACTTTCGCTCATCGCCGCATGTTGATCATTACAGGACATACCCAGATGTATACTCATCACAGCTTCACAAGTTCTTGGGTTGCTGTGGATTGGTGCATATGGCAGATAATTTGTGCTGA

Protein sequence

MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKISLSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTWNRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETFVLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKFLGCCGLVHMADNLC
Homology
BLAST of Lag0013417 vs. NCBI nr
Match: XP_038883889.1 (transmembrane protein 53 [Benincasa hispida])

HSP 1 Score: 666.8 bits (1719), Expect = 1.2e-187
Identity = 338/397 (85.14%), Postives = 362/397 (91.18%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNRRLFSKTIP SRLV+SPES+ SA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRRLFSKTIPSSRLVSSPESDRSAIFRQSSTYFHLSPSRKYLGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG +PSNFLSSLPS+QSFGSQF  D+ NS LSDSNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSSPSNFLSSLPSLQSFGSQFASDYSNSVLSDSNGSAWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  ++GVLG EKG+ TVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDP E
Sbjct: 121 NRASESAIGNDLGVLGAEKGTVTVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLSD EESD DR LIFH FSNTGWFVYGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSDGEESDNDRCLIFHAFSNTGWFVYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLL+KIKGCI DSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEE+DK+P  +ET 
Sbjct: 241 KDLLKKIKGCIFDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEVDKRPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
           VLSSLEKFFSVALKLPDVDKRL  IVSVLTENQP YPELYLYSSGD+VV  ESIELLIEK
Sbjct: 301 VLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVLSESIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKFL 398
           RKKTGRKVLS++FR+SPHVDHYRTYPD+Y+SQLHKFL
Sbjct: 361 RKKTGRKVLSYNFRTSPHVDHYRTYPDIYASQLHKFL 397

BLAST of Lag0013417 vs. NCBI nr
Match: XP_008440723.1 (PREDICTED: transmembrane protein 53 [Cucumis melo] >KAA0036219.1 transmembrane protein 53 [Cucumis melo var. makuwa])

HSP 1 Score: 663.7 bits (1711), Expect = 1.0e-186
Identity = 335/397 (84.38%), Postives = 360/397 (90.68%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESN SA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+ EESDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEEIDKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLPDVDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKFL 398
           R KTGRK+ S++F  SPHVDHYRTYPD+YSSQLHKFL
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFL 397

BLAST of Lag0013417 vs. NCBI nr
Match: TYK12615.1 (transmembrane protein 53 [Cucumis melo var. makuwa])

HSP 1 Score: 662.1 bits (1707), Expect = 3.0e-186
Identity = 334/396 (84.34%), Postives = 359/396 (90.66%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESN SA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+ EESDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEEIDKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLPDVDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKF 397
           R KTGRK+ S++F  SPHVDHYRTYPD+YSSQLHKF
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKF 396

BLAST of Lag0013417 vs. NCBI nr
Match: XP_011658015.1 (transmembrane protein 53 [Cucumis sativus])

HSP 1 Score: 656.4 bits (1692), Expect = 1.7e-184
Identity = 336/397 (84.63%), Postives = 357/397 (89.92%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNR LFSKTIP SRLV+SPES  SA+FRQS TY H  PSR   GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGLFSKTIPQSRLVSSPESYRSAIFRQSSTYFHFSPSRKNLGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHC SS FGSS+SSLG  PSNFL SLPS+QSFGSQF  D+F+SFL DSNGS WTW
Sbjct: 61  LSLNLSHCCSSSFGSSASSLGSIPSNFLYSLPSLQSFGSQFASDYFSSFLFDSNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVGVL GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGVLRGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+ EESDKDRSLIFHTFSNTGWFVYGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEEIDKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLPDVDKRL  IVSVLTENQP YPELYLYSSGD+VVP+ SIELLI+K
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPPYPELYLYSSGDKVVPYTSIELLIKK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKFL 398
           R  TGRKV S++F +SPHVDHYRTYPD+YS QLHKFL
Sbjct: 361 RMMTGRKVFSYNFGTSPHVDHYRTYPDIYSLQLHKFL 397

BLAST of Lag0013417 vs. NCBI nr
Match: XP_023543057.1 (transmembrane protein 53 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 655.2 bits (1689), Expect = 3.7e-184
Identity = 333/397 (83.88%), Postives = 356/397 (89.67%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNR LF+KTIP SRLV+ PESN +A+ RQS TY H PP R   G K S
Sbjct: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPESNRTAILRQSSTYFHFPP-RKCLGLKFS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGS++SS+G +  NFLSSLPS+QS GSQF PD+ NSFLSDSNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSTASSIGSSAFNFLSSLPSLQSLGSQFAPDYSNSFLSDSNGSAWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG LGGEKG ATVVLLGWLG+KTKHLRRYVEWYNARGINALTFV+DP E
Sbjct: 121 NRASESAIGNNVGALGGEKGEATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLA ELISWLSD EE+ KDR LIFHTFSNTGWFVYGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLATELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQ+WAAGFSAAILKKNSSSTSP+V G+EIDKKP  +ETF
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETF 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
           VLSSLE FFSVALK PDVDKRLKKIVS+LTE QP YPELYLYSSGD+VVPFESIELLIEK
Sbjct: 301 VLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKFL 398
           RKKTG KVLS++F SSPHVDHYRTYPD+YSSQLHKFL
Sbjct: 361 RKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFL 396

BLAST of Lag0013417 vs. ExPASy Swiss-Prot
Match: Q6P2H8 (Transmembrane protein 53 OS=Homo sapiens OX=9606 GN=TMEM53 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.7e-19
Identity = 85/265 (32.08%), Postives = 121/265 (45.66%), Query Frame = 0

Query: 138 EKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDL 197
           E     V+LLGW G K K+L +Y   Y+ RG   + +   P   ++F+ S  +   +  L
Sbjct: 32  ETRQPVVILLGWGGCKDKNLAKYSAIYHKRGCIVIRYTA-PWHMVFFSESLGIPS-LRVL 91

Query: 198 AVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLEKIKGCIVDSGGG 257
           A +L+  L D E   +   L+FH FSN G  +Y  +LE+LQ R+    ++ G I DS  G
Sbjct: 92  AQKLLELLFDYE--IEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIFDSAPG 151

Query: 258 DPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETFVLSSLEKFFSVALKLPD 317
           D  +  V A    AAIL++ ++    ++               V  +L       L  P 
Sbjct: 152 D--SNLVGALRALAAILERRAAMLRLLL--------------LVAFALVVVLFHVLLAPI 211

Query: 318 VDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVL--SHDFRS 377
                      L +    +PELYLYS  D VV    IE ++E R    R+VL  S DF S
Sbjct: 212 TALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEAR--LARRVLARSVDFVS 271

Query: 378 SPHVDHYRTYPDVYSSQLHKFLGCC 401
           S HV H R YP  Y+S    F+  C
Sbjct: 272 SAHVSHLRDYPTYYTSLCVDFMRNC 274

BLAST of Lag0013417 vs. ExPASy Swiss-Prot
Match: Q9D0Z3 (Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 4.3e-18
Identity = 78/263 (29.66%), Postives = 119/263 (45.25%), Query Frame = 0

Query: 139 KGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDLA 198
           K    V+LLGW G + K+L +Y   Y+ RG   + +   P   ++F+ S  +   +  +A
Sbjct: 31  KQQPVVILLGWGGCRDKNLAKYSAIYHKRGCIVIRYTA-PWHMVFFSESLGIPS-LRVIA 90

Query: 199 VELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLE-KIKGCIVDSGGG 258
            +L+  L D E   +   L+FH FSN G  +Y  +LE+LQ  +      + G I DSG G
Sbjct: 91  QKLLELLFDYE--IEREPLLFHVFSNAGVMLYRYVLELLQTHQRFRHLHVVGTIFDSGPG 150

Query: 259 DPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETFVLSSLEKFFSVALKLPD 318
           D  +  + A    A IL++  +    ++           +  F L  +   F +A   P 
Sbjct: 151 D--SNLIGALRALATILERRPAVLRLLL-----------LAAFALVVILFHFLLA---PF 210

Query: 319 VDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSSP 378
                      L ++   +PELYLYS  D+VV    +E ++E R      V   DF SS 
Sbjct: 211 TALFHTHFYDRLQDSGSCWPELYLYSRADKVVSARDVERMVEARLAHQVMVRGVDFVSSA 270

Query: 379 HVDHYRTYPDVYSSQLHKFLGCC 401
           HV H R YP  Y+S    F+  C
Sbjct: 271 HVSHLRDYPTYYTSLCVDFMHNC 273

BLAST of Lag0013417 vs. ExPASy Swiss-Prot
Match: Q6DHN0 (Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 5.6e-18
Identity = 73/264 (27.65%), Postives = 127/264 (48.11%), Query Frame = 0

Query: 144 VVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDLAVELIS 203
           V+LLGW GS+ KHL +Y   YN +G   L +   P + ++ + S   ++ +   A +L+ 
Sbjct: 30  VILLGWAGSRDKHLAKYSSIYNEQGCTTLRYTA-PLKTVFISESLGYKE-LRSTAHKLLE 89

Query: 204 WLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLEK-IKGCIVDSGGGDPLNP 263
            L D E   ++  + FH FSN G+ +Y  ++E+L   K      + G +VDS  G   + 
Sbjct: 90  LLYDYEV--ENNPIFFHVFSNGGFMLYRYMVELLHSHKQFSTLCVVGTVVDSAPG---SQ 149

Query: 264 QVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETFVLSSLEKFFSVALKL------P 323
            V  A      L+   ++  P V+          ++ F+L+     F+VA+ L      P
Sbjct: 150 NVVGA------LRALKTTLGPKVN--------VLLQYFLLA----LFAVAVFLLRIVLYP 209

Query: 324 DVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSS 383
                 +     + E+   +P++YLYS  DRV+ +  +E +++  ++ G  V S DF + 
Sbjct: 210 LTKYFHRNHYDAMMEHPAPWPQMYLYSRADRVIRYRDVEKMVKGLQEKGLMVESFDFITP 268

Query: 384 PHVDHYRTYPDVYSSQLHKFLGCC 401
            HV  +R  P+ YS++   FL  C
Sbjct: 270 AHVSLFRDCPEDYSNRCRTFLSHC 268

BLAST of Lag0013417 vs. ExPASy Swiss-Prot
Match: Q2TBP5 (Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 9.6e-18
Identity = 76/258 (29.46%), Postives = 115/258 (44.57%), Query Frame = 0

Query: 144 VVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDLAVELIS 203
           V+LLGW G   K+L +Y   Y+ RG   + +   P   ++F+ +  +   +  LA +L+ 
Sbjct: 38  VILLGWGGCSDKNLAKYSAIYHKRGCIVIRYTA-PWHMVFFSETLGIPS-LRVLAQKLLE 97

Query: 204 WLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLE-KIKGCIVDSGGGDPLNP 263
            L D E   +   L+FH FSN G  +Y  +LE+LQ  +     ++ G I DSG GD  + 
Sbjct: 98  LLFDYEV--EKEPLLFHVFSNAGVMLYRYVLELLQTHQRFCHLRVVGTIFDSGPGD--SN 157

Query: 264 QVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETFVLSSLEKFFSVALKLPDVDKRL 323
            + A    A +L+   ++   ++               V  +L  F    L  P      
Sbjct: 158 LLGALRALAVVLEHRPAALRLLL--------------LVAFTLVAFLFHVLLAPLTALFH 217

Query: 324 KKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSSPHVDHY 383
                 L +    +PELYLYS  D VV    +E ++E R      V S DF SS HV H 
Sbjct: 218 THFYDRLLDAASRWPELYLYSRADEVVLARDVERMVEARLAHQVLVRSVDFVSSAHVSHL 275

Query: 384 RTYPDVYSSQLHKFLGCC 401
           R YP  Y++    F+  C
Sbjct: 278 RDYPTYYTTLCINFMHSC 275

BLAST of Lag0013417 vs. ExPASy Swiss-Prot
Match: Q6DJC8 (Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.1e-12
Identity = 67/263 (25.48%), Postives = 115/263 (43.73%), Query Frame = 0

Query: 144 VVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRISDL---AVE 203
           V+LLGW G K ++L +Y   Y+ +G   + +        W A+        S L   A +
Sbjct: 30  VILLGWGGCKDQYLAKYSAIYHNQGCTVIKYTA-----AWNAVFISESLGFSSLREDAKK 89

Query: 204 LISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLE-KIKGCIVDSGGGDP 263
           L+  L D E   +   ++FH FSN G+ +Y  I+E+L     L +  + G I DS  G  
Sbjct: 90  LLELLFDYE--IEKSPILFHVFSNGGFMLYRYIVELLHSHCRLNKLHVVGTIFDSAPG-- 149

Query: 264 LNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETFVLSSLE-KFFSVALKLPDV 323
            N  V  +  +   + + S++ +              +    L++       + + L  V
Sbjct: 150 -NRNVIGSVRALDTILRTSTNNA--------------IRFLALAAFAIMVIILRIVLYPV 209

Query: 324 DKRL-KKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEKRKKTGRKVLSHDFRSSP 383
            K L +     + ++   +P+LYLYS  D ++ +  +E +I  R++      + DF  S 
Sbjct: 210 TKFLHENHYDAMKKDSSRWPQLYLYSRADPIISYIDVESMIAARRRCCLPTEALDFGKSE 268

Query: 384 HVDHYRTYPDVYSSQLHKFLGCC 401
           HV H+R +P  YS     FL  C
Sbjct: 270 HVSHFRRFPHRYSEMCTSFLRDC 268

BLAST of Lag0013417 vs. ExPASy TrEMBL
Match: A0A5A7T4A9 (Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G00160 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 5.0e-187
Identity = 335/397 (84.38%), Postives = 360/397 (90.68%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESN SA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+ EESDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEEIDKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLPDVDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKFL 398
           R KTGRK+ S++F  SPHVDHYRTYPD+YSSQLHKFL
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFL 397

BLAST of Lag0013417 vs. ExPASy TrEMBL
Match: A0A1S3B1C0 (transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103485055 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 5.0e-187
Identity = 335/397 (84.38%), Postives = 360/397 (90.68%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESN SA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+ EESDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEEIDKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLPDVDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKFL 398
           R KTGRK+ S++F  SPHVDHYRTYPD+YSSQLHKFL
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKFL 397

BLAST of Lag0013417 vs. ExPASy TrEMBL
Match: A0A5D3CM53 (Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002010 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.5e-186
Identity = 334/396 (84.34%), Postives = 359/396 (90.66%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNR  FSKTIP SRLV+ PESN SA+FRQS TY H  PSR + GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGFFSKTIPQSRLVSFPESNRSAIFRQSSTYFHFSPSRKYFGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGSS+SSLG  PSNFLSSLPS+QSFGSQ   D+ +SFLS+SNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSSASSLGSIPSNFLSSLPSLQSFGSQLASDYLSSFLSESNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVG+L GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGILQGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+ EESDKDRSLIFHTFSNTGWF+YGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFIYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEEIDKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLPDVDKRL  IVSVLTENQP YPELYLYSSGD+VVP++SIELLIEK
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPSYPELYLYSSGDKVVPYKSIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKF 397
           R KTGRK+ S++F  SPHVDHYRTYPD+YSSQLHKF
Sbjct: 361 RMKTGRKIFSYNFGMSPHVDHYRTYPDIYSSQLHKF 396

BLAST of Lag0013417 vs. ExPASy TrEMBL
Match: A0A0A0KGH8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G502770 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 8.0e-185
Identity = 336/397 (84.63%), Postives = 357/397 (89.92%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNR LFSKTIP SRLV+SPES  SA+FRQS TY H  PSR   GSKIS
Sbjct: 1   MEAPVRAFRPCVLNRGLFSKTIPQSRLVSSPESYRSAIFRQSSTYFHFSPSRKNLGSKIS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHC SS FGSS+SSLG  PSNFL SLPS+QSFGSQF  D+F+SFL DSNGS WTW
Sbjct: 61  LSLNLSHCCSSSFGSSASSLGSIPSNFLYSLPSLQSFGSQFASDYFSSFLFDSNGSDWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRASESAI  NVGVL GEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTF+VDP E
Sbjct: 121 NRASESAIGNNVGVLRGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFIVDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLAVELISWLS+ EESDKDRSLIFHTFSNTGWFVYGAILEILQGR
Sbjct: 181 FLWFALSRKVEQRISDLAVELISWLSEGEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSS SP+V+GEEIDKKP  +ET 
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSASPMVNGEEIDKKPLLLETI 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
            LSSLEKFFSVALKLPDVDKRL  IVSVLTENQP YPELYLYSSGD+VVP+ SIELLI+K
Sbjct: 301 FLSSLEKFFSVALKLPDVDKRLNNIVSVLTENQPPYPELYLYSSGDKVVPYTSIELLIKK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKFL 398
           R  TGRKV S++F +SPHVDHYRTYPD+YS QLHKFL
Sbjct: 361 RMMTGRKVFSYNFGTSPHVDHYRTYPDIYSLQLHKFL 397

BLAST of Lag0013417 vs. ExPASy TrEMBL
Match: A0A6J1IMV1 (transmembrane protein 53 OS=Cucurbita maxima OX=3661 GN=LOC111478463 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 6.8e-184
Identity = 331/397 (83.38%), Postives = 356/397 (89.67%), Query Frame = 0

Query: 1   MEAPVRAFRPYVLNRRLFSKTIPPSRLVASPESNPSALFRQSPTYLHPPPSRNFPGSKIS 60
           MEAPVRAFRP VLNR LF+KTIP SRLV+ PE N +A+FRQS TY H PP R   G K S
Sbjct: 1   MEAPVRAFRPCVLNRHLFTKTIPQSRLVSFPEFNRTAIFRQSSTYFHFPP-RKCLGLKFS 60

Query: 61  LSLNLSHCSSSPFGSSSSSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTW 120
           LSLNLSHCSSS FGS++SS+G N  NFLSSLPS+QSFGSQF PD+ NSFL DSNGS WTW
Sbjct: 61  LSLNLSHCSSSSFGSTASSIGSNAFNFLSSLPSLQSFGSQFAPDYSNSFLPDSNGSAWTW 120

Query: 121 NRASESAIKGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTE 180
           NRAS+SAI  NVG LGGEKG+ATVVLLGWLG+KTKHLRRYVEWYNARGINALTFV+DP E
Sbjct: 121 NRASDSAIGINVGALGGEKGAATVVLLGWLGAKTKHLRRYVEWYNARGINALTFVLDPRE 180

Query: 181 FLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGR 240
           FLWFALSR+VEQRISDLA ELISWLSD EE+ KDR LIFHTFSNTGWFVYGA+LEI QGR
Sbjct: 181 FLWFALSRKVEQRISDLAAELISWLSDGEENHKDRRLIFHTFSNTGWFVYGAVLEIFQGR 240

Query: 241 KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKNSSSTSPVVDGEEIDKKPPWMETF 300
           KDLLEKIKGCIVDSGGGDPLNPQ+WAAGFSAAILKKNSSSTSP+V G+EIDKKP  +ETF
Sbjct: 241 KDLLEKIKGCIVDSGGGDPLNPQIWAAGFSAAILKKNSSSTSPMVSGKEIDKKPLVLETF 300

Query: 301 VLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEK 360
           VLSSLE FFSVALK PDVDKRLKKIVS+LTE QP YPELYLYSSGD+VVPFESIELLIEK
Sbjct: 301 VLSSLENFFSVALKFPDVDKRLKKIVSLLTEYQPSYPELYLYSSGDKVVPFESIELLIEK 360

Query: 361 RKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKFL 398
           RKKTG KVLS++F SSPHVDHYRTYPD+YSSQLHKFL
Sbjct: 361 RKKTGTKVLSYNFGSSPHVDHYRTYPDIYSSQLHKFL 396

BLAST of Lag0013417 vs. TAIR 10
Match: AT2G18245.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 2.3e-91
Identity = 175/333 (52.55%), Postives = 228/333 (68.47%), Query Frame = 0

Query: 78  SSLGFNPSNFLSSLPSIQSFGSQFEPDHFNSFLSDSNGSTWTWNRASESAI-KGNVGVLG 137
           SSL  N + F S      +F  +    H N F S+ N     WNRA       GN  + G
Sbjct: 57  SSLPANANLFRSLFFLTSTFRLENTAVHANPFRSEPNDGVAVWNRAPRIVNGGGNAAIFG 116

Query: 138 GEKG-SATVVLLGWLGSKTKHLRRYVEWYNARGINALTFVVDPTEFLWFALSRRVEQRIS 197
           G +G   TVVLLGWLG+K KHLRRYVEWYN+RGINA+TF VD  + L   L RR+E+RI+
Sbjct: 117 GNEGKETTVVLLGWLGAKAKHLRRYVEWYNSRGINAVTFTVDVRDLLRLDLGRRLERRIA 176

Query: 198 DLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAILEILQGRKDLLEKIKGCIVDSG 257
           +   EL++W+S++E+  +++ L+FH+FSNTGW VYGA+LE   GR+DL+E+IKGCI+DSG
Sbjct: 177 EFGNELVNWVSEKEDDGREKCLVFHSFSNTGWLVYGALLESFIGRQDLVERIKGCIIDSG 236

Query: 258 GGDPLNPQVWAAGFSAAILKKNSS--STSPVVDGEEID------KKPPWMETFVLSSLEK 317
           G DPL+ ++WAAGF+AAILKK SS  +T P    +E D      K+P  +E  +LSSLEK
Sbjct: 237 GADPLDTKIWAAGFTAAILKKRSSTITTEPNSPIKEEDASTPQKKEPLGIENIMLSSLEK 296

Query: 318 FFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGDRVVPFESIELLIEKRKKTGRK 377
            F + L  PDV+ RL KI+  L EN P  P+LYLYSSGD+VVP  S+EL I +++K GRK
Sbjct: 297 LFPIFLNHPDVNTRLTKIIQKLYENHPPCPQLYLYSSGDKVVPSHSVELRIREQQKIGRK 356

Query: 378 VLSHDFRSSPHVDHYRTYPDVYSSQLHKFLGCC 401
           + S +F+SSPHVDHYR +PD+YSSQL  FL  C
Sbjct: 357 IHSFNFKSSPHVDHYRNFPDLYSSQLQNFLQEC 389

BLAST of Lag0013417 vs. TAIR 10
Match: AT3G19970.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 240.4 bits (612), Expect = 2.6e-63
Identity = 127/299 (42.47%), Postives = 188/299 (62.88%), Query Frame = 0

Query: 117 TWTWNRASESAI--KGNVGVLGGEKGSATVVLLGWLGSKTKHLRRYVEWYNARGINALTF 176
           ++ W+    SA+   G+   +  EK    VVLLGWLGSK KHL++Y +WY ++G + +TF
Sbjct: 137 SYRWHLPEPSAVDMSGSSSCV-SEKNRTVVVLLGWLGSKQKHLKKYADWYTSKGYHVITF 196

Query: 177 VVDPTEFLWFALSRRVEQRISDLAVELISWLSDEEESDKDRSLIFHTFSNTGWFVYGAIL 236
            +   E + + +  + E+ I  L   L  WL +E++    ++L+FHTFSNTGW  YGAIL
Sbjct: 197 TLPMNEIMSYQVGGKAEKNIESLVNHLADWLDEEQK----KNLVFHTFSNTGWLTYGAIL 256

Query: 237 EILQGR-KDLLEKIKGCIVDSGGGDPLNPQVWAAGFSAAILKKN------SSSTSPVVDG 296
           E  Q +   L+ ++KGCIVDS      +P VWA+GFSAA LKK+      S+S+S   +G
Sbjct: 257 EKFQKQDSSLMGRVKGCIVDSAPVAAADPTVWASGFSAAFLKKSSVATKGSASSSYESNG 316

Query: 297 EEIDK-KPPWMETFVLSSLEKFFSVALKLPDVDKRLKKIVSVLTENQPFYPELYLYSSGD 356
             I + KP   ET +L  LEKFF+V L LP V++RL  ++  L+  QP  P+LY+YSS D
Sbjct: 317 INISQPKPAATETALLLVLEKFFAVILNLPKVNRRLADVLDTLSTAQPSCPQLYIYSSAD 376

Query: 357 RVVPFESIELLIEKRKKTGRKVLSHDFRSSPHVDHYRTYPDVYSSQLHKF-----LGCC 401
           RV+P E +E  I +++K G +V + +F SSPHVDH+R+ P++Y+++L+ F     L CC
Sbjct: 377 RVIPVEQVESFIVEQRKAGHEVRACNFISSPHVDHFRSNPELYTAELNHFMNNFVLSCC 430

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883889.11.2e-18785.14transmembrane protein 53 [Benincasa hispida][more]
XP_008440723.11.0e-18684.38PREDICTED: transmembrane protein 53 [Cucumis melo] >KAA0036219.1 transmembrane p... [more]
TYK12615.13.0e-18684.34transmembrane protein 53 [Cucumis melo var. makuwa][more]
XP_011658015.11.7e-18484.63transmembrane protein 53 [Cucumis sativus][more]
XP_023543057.13.7e-18483.88transmembrane protein 53 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q6P2H81.7e-1932.08Transmembrane protein 53 OS=Homo sapiens OX=9606 GN=TMEM53 PE=2 SV=1[more]
Q9D0Z34.3e-1829.66Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1[more]
Q6DHN05.6e-1827.65Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1[more]
Q2TBP59.6e-1829.46Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1[more]
Q6DJC82.1e-1225.48Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7T4A95.0e-18784.38Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3B1C05.0e-18784.38transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103485055 PE=3 SV=1[more]
A0A5D3CM531.5e-18684.34Transmembrane protein 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0KGH88.0e-18584.63Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G502770 PE=3 SV=1[more]
A0A6J1IMV16.8e-18483.38transmembrane protein 53 OS=Cucurbita maxima OX=3661 GN=LOC111478463 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G18245.12.3e-9152.55alpha/beta-Hydrolases superfamily protein [more]
AT3G19970.12.6e-6342.47alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008547Protein of unknown function DUF829, TMEM53PFAMPF05705DUF829coord: 143..397
e-value: 4.8E-61
score: 206.8
IPR008547Protein of unknown function DUF829, TMEM53PANTHERPTHR12265UNCHARACTERIZEDcoord: 81..400
NoneNo IPR availablePANTHERPTHR12265:SF11ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 81..400
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 138..397

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013417.1Lag0013417.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0097573 glutathione oxidoreductase activity