Lag0013325 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013325
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionMajor facilitator superfamily protein
Locationchr1: 49366949 .. 49368902 (-)
RNA-Seq ExpressionLag0013325
SyntenyLag0013325
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCGGGCAGACGAGTCCTTGTTGCCCGGAGTCTATAAGGAAGTTGGAGCCGCTCTTCACACCGATCCAACCGGATTGGGTTCCCTCACTCTCTTCAGATCTATGGTTCAGTCTTCCTGTTATCCATTGGCCGCTTACTTAGCTGTGCACCATAACAGAGCCCATGTCATTGCCCTTGGTGCTTTTCTCTGGGCCGCCGCCACTTTCCTCGTCGCCATTTCCTCCACTTTCCTCCAGGTTCTTTTCAATCTTCCCTGTTCCATTTCCATCCTTTGACTTCTTTTTGTTTAGTTTTACGTTTATCCTTTTCTTTTCTTTTCTTTTCACTTCACTCTTTGCTGGCTTGTTTTGAAACGTGAAGATCCACTCAAGTGGTCCGTTCATGTGATTGGAGTGAAAATATGTAGAGCTGGGGAGATTCTGCACGAGTTCTTATGGGCTTTTGAAGGGGAAAAAAATTAAATGCAGATGTTTTAAATATGGGATGAAGTGAAATTCATGAAGGAAGGTTTTTATAAATGGTAGTGGTTGAATTCCAATCTCATTTCGGTAATGGAAAAAAAAAAAAAAAAAACATTGGAAATAAAATTGATCTTACATGAACAAAATACCAAATCAATCATTAAAAACTTAGCCGCAATGTGATATTCATATTAATTCAGGTGGCGATTTCAAGAGGCTTAAATGGCATCGGTCTGGCCATAGTGATACCTGCCATTCAGTCCCTCGTTGCGGATTCAACTGACGATAGCAACCGCGGCCTAGCATTTGGATGGCTTTCACTGACGTCAAGTCTCGGTTCCATAATCGGTGGGCTTTGTTCTGTATTAATGGCTTCAAAATCATTCATGGGTATTCCAGGATGGAGACTTGCTTTCCATCTGGTCGGACTCATAAGTGTCATAGTCGGCATTCTCGTCCGAATTTTCGCCAGTGACCCCCGTTTTTCCAAGATCGAAGATCATCAAATTCCAAATCGGCCGTTTTGGTCAGAGTTGAAGAATCTAATCAAAGAGTCGAAGTCCATTTTAGGCATTAAATCCTTCCAGATAATTGTCGCTCAAGGGGTTGCCGGATCTTTTCCTTGGTCTGCCCTGTCTTTTGCTCCCATGTGGCTTGAACTCATAGGCTTCTCCCATGAAAAGACTGCCTTTCTTTGGACAATCTTCACTGTCTCCTGTTCGATTGGCGCTCTTTTGGGTGGAAAAATGGGAGATACCCTGGCTAAACACTGGCCTAATTCTGGACGAATAGTCCTATCTCAGATAAGCTCTGCCTCAGCCGTTCCTCTTGCTGCAATCTTGCTGTTGGGCTTGCCTGATGATCCATCCACCTCAATTCTTCATGGCCTGGGCTTGTTCATAATGGGGTTGAGCATCTCTTGGAATGCTTCAGCGACTAACAAGTATGCATTTTTCATAAGAAGAAAAAAGAAAAATTTAGAGAGAAAATTTCCGCTTTTCTTACGTAGGATTGTTTTTTCAGTCCAATATTTGCAGAGATAGTCCCGGAGAAGTCTCGCACGAGCATCTATGCTTTGGATCGTTCATTTGAGTCCATATTGGCATCGTTTGCTCCTCCTGTTGTTGGGGTTTTGGCACAGCATGTTTACGGTTACAAACCAATAACAGAGGGACCATCGGACTCTGCCCAGATTAAAACAGACAAAGACAATGCAAAATCACTGGCCAAGGCGCTGTACACAGCCATTGGCGTACCAATTTCACTGTGCTGCTTCATTTACTCTTTTCTTTACTGCTCCTACCCAAGAGACCGAGAGCGAGCGCGGATGCATGCGCTGATCGAGTCTGAAATGCTACAAGTAGAGGCAAATGATTTTATTAAAGAAATTGAAGGAATTGATGAGATTCATATAAGCTATGAAGTGGATGAGAGCCTCGATTTTATTGACTGTGATGAAAAGCAACTTCTCCATCATCAAGTAGCATAA

mRNA sequence

ATGGAGCGGGCAGACGAGTCCTTGTTGCCCGGAGTCTATAAGGAAGTTGGAGCCGCTCTTCACACCGATCCAACCGGATTGGGTTCCCTCACTCTCTTCAGATCTATGGTTCAGTCTTCCTGTTATCCATTGGCCGCTTACTTAGCTGTGCACCATAACAGAGCCCATGTCATTGCCCTTGGTGCTTTTCTCTGGGCCGCCGCCACTTTCCTCGTCGCCATTTCCTCCACTTTCCTCCAGGTGGCGATTTCAAGAGGCTTAAATGGCATCGGTCTGGCCATAGTGATACCTGCCATTCAGTCCCTCGTTGCGGATTCAACTGACGATAGCAACCGCGGCCTAGCATTTGGATGGCTTTCACTGACGTCAAGTCTCGGTTCCATAATCGGTGGGCTTTGTTCTGTATTAATGGCTTCAAAATCATTCATGGGTATTCCAGGATGGAGACTTGCTTTCCATCTGGTCGGACTCATAAGTGTCATAGTCGGCATTCTCGTCCGAATTTTCGCCAGTGACCCCCGTTTTTCCAAGATCGAAGATCATCAAATTCCAAATCGGCCGTTTTGGTCAGAGTTGAAGAATCTAATCAAAGAGTCGAAGTCCATTTTAGGCATTAAATCCTTCCAGATAATTGTCGCTCAAGGGGTTGCCGGATCTTTTCCTTGGTCTGCCCTGTCTTTTGCTCCCATGTGGCTTGAACTCATAGGCTTCTCCCATGAAAAGACTGCCTTTCTTTGGACAATCTTCACTGTCTCCTGTTCGATTGGCGCTCTTTTGGGTGGAAAAATGGGAGATACCCTGGCTAAACACTGGCCTAATTCTGGACGAATAGTCCTATCTCAGATAAGCTCTGCCTCAGCCGTTCCTCTTGCTGCAATCTTGCTGTTGGGCTTGCCTGATGATCCATCCACCTCAATTCTTCATGGCCTGGGCTTGTTCATAATGGGGTTGAGCATCTCTTGGAATGCTTCAGCGACTAACAATCCAATATTTGCAGAGATAGTCCCGGAGAAGTCTCGCACGAGCATCTATGCTTTGGATCGTTCATTTGAGTCCATATTGGCATCGTTTGCTCCTCCTGTTGTTGGGGTTTTGGCACAGCATGTTTACGGTTACAAACCAATAACAGAGGGACCATCGGACTCTGCCCAGATTAAAACAGACAAAGACAATGCAAAATCACTGGCCAAGGCGCTGTACACAGCCATTGGCGTACCAATTTCACTGTGCTGCTTCATTTACTCTTTTCTTTACTGCTCCTACCCAAGAGACCGAGAGCGAGCGCGGATGCATGCGCTGATCGAGTCTGAAATGCTACAAGTAGAGGCAAATGATTTTATTAAAGAAATTGAAGGAATTGATGAGATTCATATAAGCTATGAAGTGGATGAGAGCCTCGATTTTATTGACTGTGATGAAAAGCAACTTCTCCATCATCAAGTAGCATAA

Coding sequence (CDS)

ATGGAGCGGGCAGACGAGTCCTTGTTGCCCGGAGTCTATAAGGAAGTTGGAGCCGCTCTTCACACCGATCCAACCGGATTGGGTTCCCTCACTCTCTTCAGATCTATGGTTCAGTCTTCCTGTTATCCATTGGCCGCTTACTTAGCTGTGCACCATAACAGAGCCCATGTCATTGCCCTTGGTGCTTTTCTCTGGGCCGCCGCCACTTTCCTCGTCGCCATTTCCTCCACTTTCCTCCAGGTGGCGATTTCAAGAGGCTTAAATGGCATCGGTCTGGCCATAGTGATACCTGCCATTCAGTCCCTCGTTGCGGATTCAACTGACGATAGCAACCGCGGCCTAGCATTTGGATGGCTTTCACTGACGTCAAGTCTCGGTTCCATAATCGGTGGGCTTTGTTCTGTATTAATGGCTTCAAAATCATTCATGGGTATTCCAGGATGGAGACTTGCTTTCCATCTGGTCGGACTCATAAGTGTCATAGTCGGCATTCTCGTCCGAATTTTCGCCAGTGACCCCCGTTTTTCCAAGATCGAAGATCATCAAATTCCAAATCGGCCGTTTTGGTCAGAGTTGAAGAATCTAATCAAAGAGTCGAAGTCCATTTTAGGCATTAAATCCTTCCAGATAATTGTCGCTCAAGGGGTTGCCGGATCTTTTCCTTGGTCTGCCCTGTCTTTTGCTCCCATGTGGCTTGAACTCATAGGCTTCTCCCATGAAAAGACTGCCTTTCTTTGGACAATCTTCACTGTCTCCTGTTCGATTGGCGCTCTTTTGGGTGGAAAAATGGGAGATACCCTGGCTAAACACTGGCCTAATTCTGGACGAATAGTCCTATCTCAGATAAGCTCTGCCTCAGCCGTTCCTCTTGCTGCAATCTTGCTGTTGGGCTTGCCTGATGATCCATCCACCTCAATTCTTCATGGCCTGGGCTTGTTCATAATGGGGTTGAGCATCTCTTGGAATGCTTCAGCGACTAACAATCCAATATTTGCAGAGATAGTCCCGGAGAAGTCTCGCACGAGCATCTATGCTTTGGATCGTTCATTTGAGTCCATATTGGCATCGTTTGCTCCTCCTGTTGTTGGGGTTTTGGCACAGCATGTTTACGGTTACAAACCAATAACAGAGGGACCATCGGACTCTGCCCAGATTAAAACAGACAAAGACAATGCAAAATCACTGGCCAAGGCGCTGTACACAGCCATTGGCGTACCAATTTCACTGTGCTGCTTCATTTACTCTTTTCTTTACTGCTCCTACCCAAGAGACCGAGAGCGAGCGCGGATGCATGCGCTGATCGAGTCTGAAATGCTACAAGTAGAGGCAAATGATTTTATTAAAGAAATTGAAGGAATTGATGAGATTCATATAAGCTATGAAGTGGATGAGAGCCTCGATTTTATTGACTGTGATGAAAAGCAACTTCTCCATCATCAAGTAGCATAA

Protein sequence

MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIALGAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLSLTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKIEDHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFSHEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGLPDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFAPPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLYCSYPRDRERARMHALIESEMLQVEANDFIKEIEGIDEIHISYEVDESLDFIDCDEKQLLHHQVA
Homology
BLAST of Lag0013325 vs. NCBI nr
Match: KAG7034191.1 (norA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 767.7 bits (1981), Expect = 6.0e-218
Identity = 388/489 (79.35%), Postives = 440/489 (89.98%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRS+VQSSCYPLAAYLA+HHNRAHVIAL
Sbjct: 17  MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSLVQSSCYPLAAYLAMHHNRAHVIAL 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVA+SSTFLQVAISRGLNGIGLAIV+P+IQSLVADSTDDSNRGLAFGWL 
Sbjct: 77  GAFLWAAATFLVALSSTFLQVAISRGLNGIGLAIVVPSIQSLVADSTDDSNRGLAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKIE- 180
           LT +LGSIIGGL SVLMAS SFMGIPGWR+AFHLVGLIS+IVG+LV +FA DPRFS+I+ 
Sbjct: 137 LTGNLGSIIGGLSSVLMASTSFMGIPGWRIAFHLVGLISIIVGMLVWLFADDPRFSEIDG 196

Query: 181 -DHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 240
                P +PFWSE+K+L+KE++S+LGI+SFQIIVAQGVAGSFPWSALSFAPMWLELIGFS
Sbjct: 197 TTKDQPRKPFWSEMKDLVKEARSVLGIQSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 256

Query: 241 HEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGL 300
           H+KT FLWT+F ++ S+G L GG+MGD L+K +PNSGRIVLSQISS SA+PLAAILLL L
Sbjct: 257 HKKTGFLWTLFIIAGSLGGLFGGRMGDILSKRFPNSGRIVLSQISSGSAIPLAAILLLVL 316

Query: 301 PDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFA 360
           PDDPST+ LHGL LFIMG S+SWN+ ATNNPIFAEIVPEKSRTSIYALDRSFESIL+SFA
Sbjct: 317 PDDPSTAFLHGLVLFIMGFSMSWNSPATNNPIFAEIVPEKSRTSIYALDRSFESILSSFA 376

Query: 361 PPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLY 420
           PPVVGVLAQHVYGYKP+  G SDSAQI+ D++NAKSLA+ALYTAIG+P+SLCCFIYSFLY
Sbjct: 377 PPVVGVLAQHVYGYKPVARGSSDSAQIEADRENAKSLARALYTAIGIPMSLCCFIYSFLY 436

Query: 421 CSYPRDRERARMHALIESEMLQVEAN----DFIKEIEGID--EIHISYEVDESLDFIDCD 480
           CSYPRDRERARMHALI+SEMLQ+E+        K+++  D  EI + YE+++ LDF D D
Sbjct: 437 CSYPRDRERARMHALIDSEMLQLESTTTTISMAKDVDDKDQTEIDLIYEIEDGLDFNDSD 496

Query: 481 EKQLLHHQV 482
           E  LL+HQ+
Sbjct: 497 ENHLLNHQL 505

BLAST of Lag0013325 vs. NCBI nr
Match: XP_022950940.1 (uncharacterized protein LOC111453885 [Cucurbita moschata] >KAG6604027.1 hypothetical protein SDJN03_04636, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 767.3 bits (1980), Expect = 7.8e-218
Identity = 389/489 (79.55%), Postives = 440/489 (89.98%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRS+VQSSCYPLAAYLA+HHNRAHVIAL
Sbjct: 17  MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSLVQSSCYPLAAYLAMHHNRAHVIAL 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVA+SSTFLQVAISRGLNGIGLAIV+P+IQSLVADSTDDSNRGLAFGWL 
Sbjct: 77  GAFLWAAATFLVALSSTFLQVAISRGLNGIGLAIVVPSIQSLVADSTDDSNRGLAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKIE- 180
           LT +LGSIIGGL SVLMAS SFMGIPGWR+AFHLVGLISVIVG+LV +FA DPRFS+I+ 
Sbjct: 137 LTGNLGSIIGGLSSVLMASTSFMGIPGWRIAFHLVGLISVIVGMLVWLFADDPRFSEIDG 196

Query: 181 -DHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 240
                P +PFWSE+K+L+KE++S+LGI+SFQIIVAQGVAGSFPWSALSFAPMWLELIGFS
Sbjct: 197 TTKDQPRKPFWSEMKDLVKEARSVLGIQSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 256

Query: 241 HEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGL 300
           H+KT FLWT+F ++ S+G L GG+MGD L+K +PNSGRIVLSQISS SA+PLAAILLL L
Sbjct: 257 HKKTGFLWTLFIIAGSLGGLFGGRMGDILSKRFPNSGRIVLSQISSGSAIPLAAILLLVL 316

Query: 301 PDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFA 360
           PDDPST+ LHGL LFIMG S+SWN+ ATNNPIFAEIVPEKSRTSIYALDRSFESIL+SFA
Sbjct: 317 PDDPSTAFLHGLVLFIMGFSMSWNSPATNNPIFAEIVPEKSRTSIYALDRSFESILSSFA 376

Query: 361 PPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLY 420
           PPVVGVLAQHVYGYKP+  G SDSAQI+ D++NAKSLA+ALYTAIG+P+SLCCFIYSFLY
Sbjct: 377 PPVVGVLAQHVYGYKPVARGSSDSAQIEADRENAKSLARALYTAIGIPMSLCCFIYSFLY 436

Query: 421 CSYPRDRERARMHALIESEMLQVEAN----DFIKEIEGID--EIHISYEVDESLDFIDCD 480
           CSYPRDRERARMHALI+SEMLQ+E+        K+++  D  EI + YE+++ LDF D D
Sbjct: 437 CSYPRDRERARMHALIDSEMLQLESTTTTISMAKDVDYKDQTEIDLIYEIEDGLDFNDSD 496

Query: 481 EKQLLHHQV 482
           E  LL+HQ+
Sbjct: 497 ENHLLNHQL 505

BLAST of Lag0013325 vs. NCBI nr
Match: XP_023544539.1 (uncharacterized protein LOC111804088 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 766.1 bits (1977), Expect = 1.7e-217
Identity = 391/491 (79.63%), Postives = 439/491 (89.41%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRS+VQSSCYPLAAYLA+HHNRAHVIAL
Sbjct: 17  MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSLVQSSCYPLAAYLAMHHNRAHVIAL 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVA+SSTFLQVAISRGLNGIGLAIV+P+IQSLVADSTDDSNRGLAFGWL 
Sbjct: 77  GAFLWAAATFLVALSSTFLQVAISRGLNGIGLAIVVPSIQSLVADSTDDSNRGLAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKIE- 180
           LT +LGSIIGGL SVLMAS SFMGIPGWR+AFHLVGLISVIVG+LV +FA DPRFS+I+ 
Sbjct: 137 LTGNLGSIIGGLSSVLMASTSFMGIPGWRIAFHLVGLISVIVGMLVWLFADDPRFSEIDG 196

Query: 181 -DHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 240
                P +PFWSE+K+L+KE++S+LGI+SFQIIVAQGVAGSFPWSALSFAPMWLELIGFS
Sbjct: 197 TTKDQPRKPFWSEMKDLVKEARSVLGIQSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 256

Query: 241 HEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGL 300
           HEKT FLWT+F ++ S+G L GG+MGD L+K +PNSGRIVLSQISS SA+PLAAILLL L
Sbjct: 257 HEKTGFLWTLFIIAGSLGGLFGGRMGDILSKRFPNSGRIVLSQISSGSAIPLAAILLLVL 316

Query: 301 PDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFA 360
           PD PST+ LHGL LFIMG S+SWN+ ATNNPIFAEIVPEKSRTSIYALDRSFESIL+SFA
Sbjct: 317 PDHPSTAFLHGLVLFIMGFSMSWNSPATNNPIFAEIVPEKSRTSIYALDRSFESILSSFA 376

Query: 361 PPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLY 420
           PPVVGVLAQHVYGYKP+  G SDSAQI+ D++NAKSLA+ALYTAIG+P+SLCCFIYSFLY
Sbjct: 377 PPVVGVLAQHVYGYKPVARGSSDSAQIEADRENAKSLARALYTAIGIPMSLCCFIYSFLY 436

Query: 421 CSYPRDRERARMHALIESEMLQVEANDFI------KEIEGID--EIHISYEVDESLDFID 480
           CSYPRDRERARMHALI+SEMLQ+E+   I      K+++  D  EI + YE++ SLDF D
Sbjct: 437 CSYPRDRERARMHALIDSEMLQLESTTTITTISMAKDVDDKDQAEIDLIYEIEGSLDFND 496

Query: 481 CDEKQLLHHQV 482
            DE  LL HQ+
Sbjct: 497 SDENHLLSHQL 507

BLAST of Lag0013325 vs. NCBI nr
Match: XP_022132543.1 (uncharacterized protein LOC111005375 [Momordica charantia])

HSP 1 Score: 765.8 bits (1976), Expect = 2.3e-217
Identity = 397/498 (79.72%), Postives = 439/498 (88.15%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADESLLPGVYKE+GAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL
Sbjct: 17  MERADESLLPGVYKELGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVA+SSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTD+SNRGLAFGWL 
Sbjct: 77  GAFLWAAATFLVALSSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDESNRGLAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKIED 180
           LT +LGSIIGGL SVL+AS SFMGIPGWR+AFHLVGLISV+VG+LV +FA+DPRFS+I+ 
Sbjct: 137 LTGNLGSIIGGLSSVLIASTSFMGIPGWRIAFHLVGLISVLVGLLVWLFANDPRFSEIDG 196

Query: 181 H--QIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 240
                  +PFWSELK+LIKESKS+LGI+SFQIIVAQGVAGSFPWS+LSFAPMWLELIGFS
Sbjct: 197 RVKDQSRKPFWSELKDLIKESKSVLGIQSFQIIVAQGVAGSFPWSSLSFAPMWLELIGFS 256

Query: 241 HEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGL 300
           HEKTAFLWT+F V+ S+G L GG+MGD  AK +PNSGRIVLSQISS SA+PLAAILLL L
Sbjct: 257 HEKTAFLWTLFIVASSLGGLFGGRMGDIFAKRFPNSGRIVLSQISSGSAIPLAAILLLVL 316

Query: 301 PDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFA 360
           PDDPST+ LHGL LFIMGLSISWN  ATNNPIFAEIVPEKSRTSIYALDRSFES+L+SFA
Sbjct: 317 PDDPSTAFLHGLVLFIMGLSISWNGPATNNPIFAEIVPEKSRTSIYALDRSFESVLSSFA 376

Query: 361 PPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLY 420
           PPVVGVLAQHVYGYKP+  G SDS QI+TD++NAKSLA+ALYTAIG+P+SLCCFIYSFLY
Sbjct: 377 PPVVGVLAQHVYGYKPVVRGSSDSVQIETDRENAKSLARALYTAIGIPMSLCCFIYSFLY 436

Query: 421 CSYPRDRERARMHALIESEMLQVEANDF-------------IKEIEGID--EIHISYEVD 480
            SYPRDRERARMHALIESEMLQ+EA++               K++   D  EI + Y V+
Sbjct: 437 RSYPRDRERARMHALIESEMLQLEASNLPFHEGDSRFLISEAKDLNNKDQTEIDLMYGVE 496

Query: 481 ESLDFIDCDEKQLLHHQV 482
           +SLD  D DEK LL HQ+
Sbjct: 497 DSLDLNDNDEKHLLQHQL 514

BLAST of Lag0013325 vs. NCBI nr
Match: XP_004143560.1 (uncharacterized protein LOC101211597 isoform X1 [Cucumis sativus] >KAE8647557.1 hypothetical protein Csa_004009 [Cucumis sativus])

HSP 1 Score: 765.4 bits (1975), Expect = 3.0e-217
Identity = 387/498 (77.71%), Postives = 441/498 (88.55%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MER DESLLPGVYKEVGAALH DPTGLGSLTLFRS+VQSSCYPLAAYLAVHHNRAHVIA+
Sbjct: 17  MERTDESLLPGVYKEVGAALHIDPTGLGSLTLFRSVVQSSCYPLAAYLAVHHNRAHVIAV 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVA+SSTF QVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWL 
Sbjct: 77  GAFLWAAATFLVALSSTFFQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKI-- 180
           LT +LGSIIGGLCS+LMAS SFMGIPGWR+AFHLVGLISVIVG+LV +FA+DP FS+I  
Sbjct: 137 LTGNLGSIIGGLCSILMASTSFMGIPGWRIAFHLVGLISVIVGLLVWVFANDPHFSEING 196

Query: 181 EDHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 240
            D   P +PFWSE+ +L+KESKS++GI+SFQIIV+QGVAGSFPWSALSFAPMWLEL+GFS
Sbjct: 197 RDKDHPRKPFWSEMMDLVKESKSVIGIQSFQIIVSQGVAGSFPWSALSFAPMWLELVGFS 256

Query: 241 HEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGL 300
           HEKT FLWT+F ++ S+G + GG++GD L+K +PNSGRIVLSQISSASAVPLAAILLL L
Sbjct: 257 HEKTGFLWTLFIIASSLGGIFGGRLGDILSKRFPNSGRIVLSQISSASAVPLAAILLLVL 316

Query: 301 PDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFA 360
           PD+PST+ LHGL LFIMG S+SWNA ATNNPIFAEIVP+KSRTSIYALDRSFESIL+SFA
Sbjct: 317 PDNPSTTFLHGLVLFIMGFSMSWNAPATNNPIFAEIVPKKSRTSIYALDRSFESILSSFA 376

Query: 361 PPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLY 420
           PPVVG+LAQHVYGYKP  +G +DS+QI+TD++NAKSLA+ALY AIG P+SLCCFIYSFLY
Sbjct: 377 PPVVGILAQHVYGYKPAAKGSTDSSQIETDRENAKSLARALYAAIGFPMSLCCFIYSFLY 436

Query: 421 CSYPRDRERARMHALIESEMLQVEAND----------FIKEIEGID-----EIHISYEVD 480
           CSYPRDRERARMHALIESEML +E++            I E +  D     E+ ++YE++
Sbjct: 437 CSYPRDRERARMHALIESEMLHLESSTSPLYEQDSQFHISEAKDFDDKDQTEVDLTYEIE 496

Query: 481 ESLDFIDCDEKQLLHHQV 482
           +SLDFID DEKQLL+HQ+
Sbjct: 497 DSLDFIDSDEKQLLNHQL 514

BLAST of Lag0013325 vs. ExPASy Swiss-Prot
Match: P39843 (Multidrug resistance protein 2 OS=Bacillus subtilis (strain 168) OX=224308 GN=blt PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 6.4e-05
Identity = 62/252 (24.60%), Postives = 106/252 (42.06%), Query Frame = 0

Query: 20  LHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIALGAFLWAAATFLVAISSTFL 79
           +H   + +G L    ++ Q    P A        R  +I LG  +++ +  +  + +   
Sbjct: 40  MHLSGSTMGYLVAAFAISQLITSPFAGRWVDRFGRKKMIILGLLIFSLSELIFGLGTHVS 99

Query: 80  QVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLSLTSSLGSIIGGLCSVLMAS 139
               SR L G+  A ++PA+ + VAD T    R  A G++S   S G IIG        +
Sbjct: 100 IFYFSRILGGVSAAFIMPAVTAYVADITTLKERSKAMGYVSAAISTGFIIG------PGA 159

Query: 140 KSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKIEDHQIPNRPFWSELKNLIKES 199
             F+   G R+ F     I++I  +   +F      S  E HQ+ +    ++  N IK+ 
Sbjct: 160 GGFIAGFGIRMPFFFASAIALIAAV-TSVFILKESLSIEERHQLSSH---TKESNFIKDL 219

Query: 200 K-SILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFSHEKTAFLWTIFT-VSCSIGA 259
           K SI  +     I+   +A         F+       GF+ +  A + TI + V+  I  
Sbjct: 220 KRSIHPVYFIAFIIVFVMAFGLSAYETVFSLFSDHKFGFTPKDIAAIITISSIVAVVIQV 279

Query: 260 LLGGKMGDTLAK 270
           LL GK+ + L +
Sbjct: 280 LLFGKLVNKLGE 281

BLAST of Lag0013325 vs. ExPASy TrEMBL
Match: A0A6J1GH66 (uncharacterized protein LOC111453885 OS=Cucurbita moschata OX=3662 GN=LOC111453885 PE=4 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.8e-218
Identity = 389/489 (79.55%), Postives = 440/489 (89.98%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRS+VQSSCYPLAAYLA+HHNRAHVIAL
Sbjct: 17  MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSLVQSSCYPLAAYLAMHHNRAHVIAL 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVA+SSTFLQVAISRGLNGIGLAIV+P+IQSLVADSTDDSNRGLAFGWL 
Sbjct: 77  GAFLWAAATFLVALSSTFLQVAISRGLNGIGLAIVVPSIQSLVADSTDDSNRGLAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKIE- 180
           LT +LGSIIGGL SVLMAS SFMGIPGWR+AFHLVGLISVIVG+LV +FA DPRFS+I+ 
Sbjct: 137 LTGNLGSIIGGLSSVLMASTSFMGIPGWRIAFHLVGLISVIVGMLVWLFADDPRFSEIDG 196

Query: 181 -DHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 240
                P +PFWSE+K+L+KE++S+LGI+SFQIIVAQGVAGSFPWSALSFAPMWLELIGFS
Sbjct: 197 TTKDQPRKPFWSEMKDLVKEARSVLGIQSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 256

Query: 241 HEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGL 300
           H+KT FLWT+F ++ S+G L GG+MGD L+K +PNSGRIVLSQISS SA+PLAAILLL L
Sbjct: 257 HKKTGFLWTLFIIAGSLGGLFGGRMGDILSKRFPNSGRIVLSQISSGSAIPLAAILLLVL 316

Query: 301 PDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFA 360
           PDDPST+ LHGL LFIMG S+SWN+ ATNNPIFAEIVPEKSRTSIYALDRSFESIL+SFA
Sbjct: 317 PDDPSTAFLHGLVLFIMGFSMSWNSPATNNPIFAEIVPEKSRTSIYALDRSFESILSSFA 376

Query: 361 PPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLY 420
           PPVVGVLAQHVYGYKP+  G SDSAQI+ D++NAKSLA+ALYTAIG+P+SLCCFIYSFLY
Sbjct: 377 PPVVGVLAQHVYGYKPVARGSSDSAQIEADRENAKSLARALYTAIGIPMSLCCFIYSFLY 436

Query: 421 CSYPRDRERARMHALIESEMLQVEAN----DFIKEIEGID--EIHISYEVDESLDFIDCD 480
           CSYPRDRERARMHALI+SEMLQ+E+        K+++  D  EI + YE+++ LDF D D
Sbjct: 437 CSYPRDRERARMHALIDSEMLQLESTTTTISMAKDVDYKDQTEIDLIYEIEDGLDFNDSD 496

Query: 481 EKQLLHHQV 482
           E  LL+HQ+
Sbjct: 497 ENHLLNHQL 505

BLAST of Lag0013325 vs. ExPASy TrEMBL
Match: A0A6J1BWJ4 (uncharacterized protein LOC111005375 OS=Momordica charantia OX=3673 GN=LOC111005375 PE=4 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 1.1e-217
Identity = 397/498 (79.72%), Postives = 439/498 (88.15%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADESLLPGVYKE+GAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL
Sbjct: 17  MERADESLLPGVYKELGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVA+SSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTD+SNRGLAFGWL 
Sbjct: 77  GAFLWAAATFLVALSSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDESNRGLAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKIED 180
           LT +LGSIIGGL SVL+AS SFMGIPGWR+AFHLVGLISV+VG+LV +FA+DPRFS+I+ 
Sbjct: 137 LTGNLGSIIGGLSSVLIASTSFMGIPGWRIAFHLVGLISVLVGLLVWLFANDPRFSEIDG 196

Query: 181 H--QIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 240
                  +PFWSELK+LIKESKS+LGI+SFQIIVAQGVAGSFPWS+LSFAPMWLELIGFS
Sbjct: 197 RVKDQSRKPFWSELKDLIKESKSVLGIQSFQIIVAQGVAGSFPWSSLSFAPMWLELIGFS 256

Query: 241 HEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGL 300
           HEKTAFLWT+F V+ S+G L GG+MGD  AK +PNSGRIVLSQISS SA+PLAAILLL L
Sbjct: 257 HEKTAFLWTLFIVASSLGGLFGGRMGDIFAKRFPNSGRIVLSQISSGSAIPLAAILLLVL 316

Query: 301 PDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFA 360
           PDDPST+ LHGL LFIMGLSISWN  ATNNPIFAEIVPEKSRTSIYALDRSFES+L+SFA
Sbjct: 317 PDDPSTAFLHGLVLFIMGLSISWNGPATNNPIFAEIVPEKSRTSIYALDRSFESVLSSFA 376

Query: 361 PPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLY 420
           PPVVGVLAQHVYGYKP+  G SDS QI+TD++NAKSLA+ALYTAIG+P+SLCCFIYSFLY
Sbjct: 377 PPVVGVLAQHVYGYKPVVRGSSDSVQIETDRENAKSLARALYTAIGIPMSLCCFIYSFLY 436

Query: 421 CSYPRDRERARMHALIESEMLQVEANDF-------------IKEIEGID--EIHISYEVD 480
            SYPRDRERARMHALIESEMLQ+EA++               K++   D  EI + Y V+
Sbjct: 437 RSYPRDRERARMHALIESEMLQLEASNLPFHEGDSRFLISEAKDLNNKDQTEIDLMYGVE 496

Query: 481 ESLDFIDCDEKQLLHHQV 482
           +SLD  D DEK LL HQ+
Sbjct: 497 DSLDLNDNDEKHLLQHQL 514

BLAST of Lag0013325 vs. ExPASy TrEMBL
Match: A0A6J1IQ70 (uncharacterized protein LOC111478440 OS=Cucurbita maxima OX=3661 GN=LOC111478440 PE=4 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.3e-215
Identity = 386/492 (78.46%), Postives = 437/492 (88.82%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRS+VQSSCYPLAAYLA+HHNRAHVIAL
Sbjct: 17  MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSLVQSSCYPLAAYLAMHHNRAHVIAL 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVA+SSTFLQVAISRGLNGIGLAIV+P+IQSLVADSTDDSNRGLAFGWL 
Sbjct: 77  GAFLWAAATFLVALSSTFLQVAISRGLNGIGLAIVVPSIQSLVADSTDDSNRGLAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKIE- 180
           LT +LGSIIGGL SVLMAS S MGIPGWR+AFHLVGLISVIVG+LV +FA DPRFS+I+ 
Sbjct: 137 LTGNLGSIIGGLSSVLMASTSVMGIPGWRIAFHLVGLISVIVGMLVWLFADDPRFSEIDG 196

Query: 181 -DHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 240
                P +PFWS++K+L+KES+S+LGI+SFQIIVAQGVAGSFPWSALSFAPMWLELIGFS
Sbjct: 197 TTKDRPRKPFWSQMKDLVKESRSVLGIQSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 256

Query: 241 HEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGL 300
           HE+T FLWT+F ++ S+G L GG+MGD L+K +PNSGRIVLSQISS SA+PLAAILLL L
Sbjct: 257 HEETGFLWTLFIIAGSLGGLFGGRMGDILSKRFPNSGRIVLSQISSGSAIPLAAILLLVL 316

Query: 301 PDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFA 360
           PDDPST+ LHGL LFIMG S+SWN+ ATNNPIFAEIVPEKSRTSIYALDRSFESIL+SFA
Sbjct: 317 PDDPSTAFLHGLVLFIMGFSMSWNSPATNNPIFAEIVPEKSRTSIYALDRSFESILSSFA 376

Query: 361 PPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLY 420
           PPVVGVLAQHVYGYKP+  G SDSA+I+ D++NAKSLA+ALYTAIG+P+SLCCFIYSFLY
Sbjct: 377 PPVVGVLAQHVYGYKPVARGSSDSAEIEADRENAKSLARALYTAIGIPMSLCCFIYSFLY 436

Query: 421 CSYPRDRERARMHALIESEMLQVEAN-------DFIKEIEGID--EIHISYEVDESLDFI 480
           CSYPRDRERARMHALI+SEMLQ+E+            +++  D  EI + YE++ SLDF 
Sbjct: 437 CSYPRDRERARMHALIDSEMLQLESTTTTTTTISMATDVDDKDQTEIDLIYEIEGSLDFN 496

Query: 481 DCDEKQLLHHQV 482
           D DE   L+HQ+
Sbjct: 497 DSDENHPLNHQL 508

BLAST of Lag0013325 vs. ExPASy TrEMBL
Match: A0A5D3CMN2 (Protein spinster OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002780 PE=4 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 3.9e-215
Identity = 387/500 (77.40%), Postives = 440/500 (88.00%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MER DESLLPGVYKEVGAALH DPTGLGSLTLFRS+VQSSCYPLAAYLAVHHNRAHVIA+
Sbjct: 87  MERTDESLLPGVYKEVGAALHIDPTGLGSLTLFRSVVQSSCYPLAAYLAVHHNRAHVIAV 146

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVAISSTF QVAISR LNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWL 
Sbjct: 147 GAFLWAAATFLVAISSTFFQVAISRALNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLQ 206

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFS---- 180
           LT +LGSIIGGLCS+LMAS SFMGIPGWR++FHLVGLISVIVG+LV IFA+DP FS    
Sbjct: 207 LTGNLGSIIGGLCSILMASTSFMGIPGWRISFHLVGLISVIVGLLVWIFANDPHFSENNG 266

Query: 181 KIEDHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIG 240
           + +DH  P +P WSE+K+L+KESKS++GI+SFQIIV+QGVAGSFPWSALSFAPMWLEL+G
Sbjct: 267 RGKDH--PRKPLWSEIKDLVKESKSVIGIQSFQIIVSQGVAGSFPWSALSFAPMWLELVG 326

Query: 241 FSHEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLL 300
           FSHEKT FLWT+F ++ S+G + GG+MGD L+K +PNSGRIVLSQISSASAVPLAAILLL
Sbjct: 327 FSHEKTGFLWTLFIIASSLGGIFGGRMGDILSKRFPNSGRIVLSQISSASAVPLAAILLL 386

Query: 301 GLPDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILAS 360
            LPD+PST+ LHGL LFIMG S+SWNA ATNNPIFAEIVP+KSRTSIYALDRSFESIL+S
Sbjct: 387 VLPDNPSTTFLHGLVLFIMGFSMSWNAPATNNPIFAEIVPKKSRTSIYALDRSFESILSS 446

Query: 361 FAPPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSF 420
           FAPPVVGVLAQHVYGYKP  +G +DS+QI+TD++NAKSLA+ALY AIG P+SLCCFIY+F
Sbjct: 447 FAPPVVGVLAQHVYGYKPAAKGSTDSSQIETDRENAKSLARALYAAIGFPMSLCCFIYTF 506

Query: 421 LYCSYPRDRERARMHALIESEMLQVEAND----------FIKEIEGID-----EIHISYE 480
           LYCSYPRDRERARMHALIESEML +E++            I E +  D     E+ ++YE
Sbjct: 507 LYCSYPRDRERARMHALIESEMLHLESSTSPLYEQDGQFHISEAKDFDDKDQTEVDLTYE 566

Query: 481 VDESLDFIDCDEKQLLHHQV 482
           +++SLDFID DEK LL+HQ+
Sbjct: 567 IEDSLDFIDNDEKHLLNHQL 584

BLAST of Lag0013325 vs. ExPASy TrEMBL
Match: A0A1S3B1J2 (LOW QUALITY PROTEIN: uncharacterized protein LOC103484977 OS=Cucumis melo OX=3656 GN=LOC103484977 PE=4 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 1.9e-214
Identity = 386/500 (77.20%), Postives = 439/500 (87.80%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MER D SLLPGVYKEVGAALH DPTGLGSLTLFRS+VQSSCYPLAAYLAVHHNRAHVIA+
Sbjct: 17  MERTDXSLLPGVYKEVGAALHIDPTGLGSLTLFRSVVQSSCYPLAAYLAVHHNRAHVIAV 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWAAATFLVAISSTF QVAISR LNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWL 
Sbjct: 77  GAFLWAAATFLVAISSTFFQVAISRALNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFS---- 180
           LT +LGSIIGGLCS+LMAS SFMGIPGWR++FHLVGLISVIVG+LV IFA+DP FS    
Sbjct: 137 LTGNLGSIIGGLCSILMASTSFMGIPGWRISFHLVGLISVIVGLLVWIFANDPHFSENNG 196

Query: 181 KIEDHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIG 240
           + +DH  P +P WSE+K+L+KESKS++GI+SFQIIV+QGVAGSFPWSALSFAPMWLEL+G
Sbjct: 197 RGKDH--PRKPLWSEIKDLVKESKSVIGIQSFQIIVSQGVAGSFPWSALSFAPMWLELVG 256

Query: 241 FSHEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLL 300
           FSHEKT FLWT+F ++ S+G + GG+MGD L+K +PNSGRIVLSQISSASAVPLAAILLL
Sbjct: 257 FSHEKTGFLWTLFIIASSLGGIFGGRMGDILSKRFPNSGRIVLSQISSASAVPLAAILLL 316

Query: 301 GLPDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILAS 360
            LPD+PST+ LHGL LFIMG S+SWNA ATNNPIFAEIVP+KSRTSIYALDRSFESIL+S
Sbjct: 317 VLPDNPSTTFLHGLVLFIMGFSMSWNAPATNNPIFAEIVPKKSRTSIYALDRSFESILSS 376

Query: 361 FAPPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSF 420
           FAPPVVGVLAQHVYGYKP  +G +DS+QI+TD++NAKSLA+ALY AIG P+SLCCFIY+F
Sbjct: 377 FAPPVVGVLAQHVYGYKPAAKGSTDSSQIETDRENAKSLARALYAAIGFPMSLCCFIYTF 436

Query: 421 LYCSYPRDRERARMHALIESEMLQVEAND----------FIKEIEGID-----EIHISYE 480
           LYCSYPRDRERARMHALIESEML +E++            I E +  D     E+ ++YE
Sbjct: 437 LYCSYPRDRERARMHALIESEMLHLESSTSPLYEQDGQFHISEAKDFDDKDQTEVDLTYE 496

Query: 481 VDESLDFIDCDEKQLLHHQV 482
           +++SLDFID DEK LL+HQ+
Sbjct: 497 IEDSLDFIDNDEKHLLNHQL 514

BLAST of Lag0013325 vs. TAIR 10
Match: AT1G78130.1 (Major facilitator superfamily protein )

HSP 1 Score: 661.0 bits (1704), Expect = 7.4e-190
Identity = 329/455 (72.31%), Postives = 392/455 (86.15%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADESLLPGVYKEVG ALHTDPTGLGSLTL RSMVQ++CYPLAAY+A+ HNRAHVIAL
Sbjct: 17  MERADESLLPGVYKEVGLALHTDPTGLGSLTLLRSMVQAACYPLAAYMAIRHNRAHVIAL 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLW+AATFLVA SSTF QVA+SR LNGIGLA+V PAIQSLVADSTDD+NRG AFGWL 
Sbjct: 77  GAFLWSAATFLVAFSSTFFQVAVSRALNGIGLALVAPAIQSLVADSTDDANRGTAFGWLQ 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSK--- 180
           LT+++GSI+GGLCSVL+A  +FMGIPGWR+AFH+VG+ISVIVG+LVR+FA+DP F K   
Sbjct: 137 LTANIGSILGGLCSVLIAPLTFMGIPGWRVAFHIVGVISVIVGVLVRVFANDPHFVKDGV 196

Query: 181 -IEDHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIG 240
            + +     +PF +E+K+L++E+ +++ I+SFQIIVAQGV GSFPWSALSFAPMWLELIG
Sbjct: 197 DVSNQPGSRKPFCTEVKDLVREADTVIKIRSFQIIVAQGVTGSFPWSALSFAPMWLELIG 256

Query: 241 FSHEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLL 300
           FSH KTAFL  +F  + S+G L GGKMGD L+   PNSGRI+L+QISSASA+PLAAILLL
Sbjct: 257 FSHGKTAFLMGLFVAASSLGGLFGGKMGDFLSTRLPNSGRIILAQISSASAIPLAAILLL 316

Query: 301 GLPDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILAS 360
            LPDDPST+ +HGL L ++GL +SWNA ATNNPIFAEIVPEKSRTS+YALD+SFESIL+S
Sbjct: 317 VLPDDPSTAAIHGLILVLLGLFVSWNAPATNNPIFAEIVPEKSRTSVYALDKSFESILSS 376

Query: 361 FAPPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSF 420
           FAPP+VG+LAQHVYGYKPI EG S S +I TD++NA SLAKALYT+IG+P++ CCFIYSF
Sbjct: 377 FAPPIVGILAQHVYGYKPIPEGSSRSTEIATDRENAASLAKALYTSIGLPMAACCFIYSF 436

Query: 421 LYCSYPRDRERARMHALIESEMLQVEANDFIKEIE 452
           LY SYP DR+RARM A I+SEM ++      ++IE
Sbjct: 437 LYRSYPLDRDRARMEAFIDSEMRELLPESSNRDIE 471

BLAST of Lag0013325 vs. TAIR 10
Match: AT5G10190.1 (Major facilitator superfamily protein )

HSP 1 Score: 659.8 bits (1701), Expect = 1.6e-189
Identity = 331/453 (73.07%), Postives = 392/453 (86.53%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADESLLPGVYKEVG ALH DPT LG+LTLFRS+VQSSCYPLAAYL+  HNRAHVIAL
Sbjct: 17  MERADESLLPGVYKEVGDALHVDPTALGTLTLFRSIVQSSCYPLAAYLSSRHNRAHVIAL 76

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           GAFLWA ATFLVA+S+TF QVA+SRGLNGIGLAIV PAIQSLVADSTDD NRG+AFGWL 
Sbjct: 77  GAFLWATATFLVAVSTTFFQVAVSRGLNGIGLAIVTPAIQSLVADSTDDYNRGMAFGWLG 136

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFS--KI 180
            TS++GSI+G +CS+L ASKSF G+ GWR+AF LV ++SVIVGILVR+FA+DP +S  KI
Sbjct: 137 FTSNIGSILGYVCSILFASKSFNGVAGWRIAFLLVAVVSVIVGILVRLFATDPHYSDRKI 196

Query: 181 EDHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIGFS 240
             H + ++PFWS++++L+KE+K ++ I SFQI VAQGV+GSFPWSAL+FAP+WLELIGFS
Sbjct: 197 TKH-VKDKPFWSDIRDLLKEAKMVIKIPSFQIFVAQGVSGSFPWSALAFAPLWLELIGFS 256

Query: 241 HEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLLGL 300
           H+ TA L T+FT+SCS+G L GG MGDTLAK +PN GRI LSQ+SS SA+PLAAILL+GL
Sbjct: 257 HKTTAVLVTLFTISCSLGGLFGGYMGDTLAKKFPNGGRIFLSQVSSGSAIPLAAILLIGL 316

Query: 301 PDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILASFA 360
           PDDPST+  HGL L IMGL ISWN +ATN PIFAEIVPE++RTSIYALDRSFESILASFA
Sbjct: 317 PDDPSTAFSHGLVLVIMGLCISWNGAATNGPIFAEIVPERARTSIYALDRSFESILASFA 376

Query: 361 PPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSFLY 420
           PP+VG+LAQ++YGYKPI EG + S +I TD+ NA SLAKALYT+IG+P+ +CC IYSFLY
Sbjct: 377 PPIVGMLAQNIYGYKPIPEGSTSSVKIDTDRANAASLAKALYTSIGIPMVICCTIYSFLY 436

Query: 421 CSYPRDRERARMHALIESEMLQVEANDFIKEIE 452
           C+YPRDR+RA+M ALIESEM Q+  N+   EIE
Sbjct: 437 CTYPRDRDRAKMQALIESEMQQL--NEEEDEIE 466

BLAST of Lag0013325 vs. TAIR 10
Match: AT4G36790.1 (Major facilitator superfamily protein )

HSP 1 Score: 369.4 bits (947), Expect = 4.4e-102
Identity = 191/443 (43.12%), Postives = 285/443 (64.33%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           MERADE+LLP VYKEV  A +  P+ LG LT  R+ VQ    PLA  L + ++R  V+A+
Sbjct: 54  MERADENLLPSVYKEVSEAFNAGPSDLGYLTFVRNFVQGLASPLAGVLVITYDRPIVLAI 113

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           G F WA +T  V  SS F+QVA+ R +NG GLAIVIPA+QS +ADS  D  RG  FG L+
Sbjct: 114 GTFCWALSTAAVGASSYFIQVALWRAVNGFGLAIVIPALQSFIADSYKDGARGAGFGMLN 173

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFS---- 180
           L  ++G I GG+ + +MA   F GIPGWR AF ++  +S ++G+LV +F  DPR +    
Sbjct: 174 LIGTIGGIGGGVVATVMAGSEFWGIPGWRCAFIMMAALSAVIGLLVFLFVVDPRKNIERE 233

Query: 181 KIEDHQIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFPWSALSFAPMWLELIG 240
           ++  H++ +   W++    +  +KS++ + +FQIIVAQG+ GSFPW+A+ F  MW ELIG
Sbjct: 234 ELMAHKMNSNSVWND---SLAAAKSVVKVSTFQIIVAQGIIGSFPWTAMVFFTMWFELIG 293

Query: 241 FSHEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQISSASAVPLAAILLL 300
           F H +TA L  +F    +IG L+GG + D +++ +PNSGR++ +Q S+   +P + ILL 
Sbjct: 294 FDHNQTAALLGVFATGGAIGTLMGGIIADKMSRIYPNSGRVMCAQFSAFMGIPFSIILLK 353

Query: 301 GLPDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRTSIYALDRSFESILAS 360
            +P   S+  +  + LF+MGL+I+W  SA N P+FAE+VP + RT IYA DR+FE   +S
Sbjct: 354 VIPQSTSSYSIFSITLFLMGLTITWCGSAVNAPMFAEVVPPRHRTMIYAFDRAFEGSFSS 413

Query: 361 FAPPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDNAKSLAKALYTAIGVPISLCCFIYSF 420
           FA P+VG+L++ ++GY      P   + ++     A +L+K L + + VP  LCC  Y+ 
Sbjct: 414 FAAPLVGILSEKLFGYDSRGIDPLKGSSVR----EADALSKGLLSMMAVPFGLCCLCYTP 473

Query: 421 LYCSYPRDRERARMHALIESEML 440
           L+  + +DRE A++ +  E+EM+
Sbjct: 474 LHFVFQKDRENAKIASSKETEMI 489

BLAST of Lag0013325 vs. TAIR 10
Match: AT2G18590.1 (Major facilitator superfamily protein )

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-80
Identity = 171/460 (37.17%), Postives = 258/460 (56.09%), Query Frame = 0

Query: 1   MERADESLLPGVYKEVGAALHTDPTGLGSLTLFRSMVQSSCYPLAAYLAVHHNRAHVIAL 60
           M+RADE L+P   KE+  A H   + +G L+  R++VQ    PLA   A+ ++R  V A 
Sbjct: 24  MQRADEKLIPSTAKELKEAFHAKLSDIGLLSFIRNIVQGLASPLAGLFAISYDRPTVFAF 83

Query: 61  GAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTDDSNRGLAFGWLS 120
           G+F W ++T    +S  F+QV +    NG+G AIV P +QS++ADS  +S+RG  FG  +
Sbjct: 84  GSFFWVSSTVATGVSRYFIQVTLGVAFNGVGHAIVYPVLQSIIADSFKESSRGFGFGLWN 143

Query: 121 LTSSLGSIIGGLCSVLMASKSFMGIPGWRLAFHLVGLISVIVGILVRIFASDPRFSKI-- 180
           L  ++G I G +   +MA   F GI GWR AF L   +S IVGILV  F SDPR  K   
Sbjct: 144 LIGTVGGIGGTVVPTVMAGHDFFGISGWRCAFILSATLSTIVGILVFFFVSDPREKKTSS 203

Query: 181 ------EDH-----------QIPNRPFWSELKNLIKESKSILGIKSFQIIVAQGVAGSFP 240
                 + H           + P+   W E    IK+   +  +++FQIIV QG+ GS P
Sbjct: 204 VIVHHDDQHERDENNGGTMMESPSSSVWKESWVAIKD---VTKLRTFQIIVLQGIVGSVP 263

Query: 241 WSALSFAPMWLELIGFSHEKTAFLWTIFTVSCSIGALLGGKMGDTLAKHWPNSGRIVLSQ 300
           W+A+ F  MW ELIGF H + A L  IF    +IG+L+GG + D +++ +PNSGR++ +Q
Sbjct: 264 WNAMLFWTMWFELIGFDHNQAALLNGIFATGQAIGSLVGGIIADKMSRVFPNSGRLICAQ 323

Query: 301 ISSASAVPLAAILLLGLPDDPSTSILHGLGLFIMGLSISWNASATNNPIFAEIVPEKSRT 360
            S       + +LL  +P   ++  +  + LF+MGL+I+W   A N+PI AEIVP K RT
Sbjct: 324 FSVFMGAMFSIVLLRMIPQSVNSFYIFLVTLFLMGLTITWCGPAINSPILAEIVPAKHRT 383

Query: 361 SIYALDRSFESILASFAPPVVGVLAQHVYGYKPITEGPSDSAQIKTDKDN---AKSLAKA 420
            +YA DR+ E   +SF  P+VG++++ ++G+        D+  I    D+   A++L K 
Sbjct: 384 MVYAFDRALEVTFSSFGAPLVGIMSEKLFGF--------DAKGIDHVNDSGREAEALGKG 443

Query: 421 LYTAIGVPISLCCFIYSFLYCSYPRDRERARMHALIESEM 439
           +   + +P  LCC  Y+ L+  + +DR+  R  +  E EM
Sbjct: 444 IMWMMALPFGLCCLCYTPLHFLFRKDRKIDRTTSSREVEM 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7034191.16.0e-21879.35norA, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022950940.17.8e-21879.55uncharacterized protein LOC111453885 [Cucurbita moschata] >KAG6604027.1 hypothet... [more]
XP_023544539.11.7e-21779.63uncharacterized protein LOC111804088 [Cucurbita pepo subsp. pepo][more]
XP_022132543.12.3e-21779.72uncharacterized protein LOC111005375 [Momordica charantia][more]
XP_004143560.13.0e-21777.71uncharacterized protein LOC101211597 isoform X1 [Cucumis sativus] >KAE8647557.1 ... [more]
Match NameE-valueIdentityDescription
P398436.4e-0524.60Multidrug resistance protein 2 OS=Bacillus subtilis (strain 168) OX=224308 GN=bl... [more]
Match NameE-valueIdentityDescription
A0A6J1GH663.8e-21879.55uncharacterized protein LOC111453885 OS=Cucurbita moschata OX=3662 GN=LOC1114538... [more]
A0A6J1BWJ41.1e-21779.72uncharacterized protein LOC111005375 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1IQ702.3e-21578.46uncharacterized protein LOC111478440 OS=Cucurbita maxima OX=3661 GN=LOC111478440... [more]
A0A5D3CMN23.9e-21577.40Protein spinster OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002... [more]
A0A1S3B1J21.9e-21477.20LOW QUALITY PROTEIN: uncharacterized protein LOC103484977 OS=Cucumis melo OX=365... [more]
Match NameE-valueIdentityDescription
AT1G78130.17.4e-19072.31Major facilitator superfamily protein [more]
AT5G10190.11.6e-18973.07Major facilitator superfamily protein [more]
AT4G36790.14.4e-10243.12Major facilitator superfamily protein [more]
AT2G18590.11.3e-8037.17Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 4..376
e-value: 6.6E-41
score: 142.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 5..423
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 9..359
e-value: 4.7E-34
score: 117.9
NoneNo IPR availablePANTHERPTHR23505SPINSTERcoord: 1..479
NoneNo IPR availablePANTHERPTHR23505:SF72OS09G0371000 PROTEINcoord: 1..479
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 1..423
score: 16.928465
IPR044770Protein spinster-likeCDDcd17328MFS_spinster_likecoord: 1..414
e-value: 6.33468E-81
score: 254.853

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013325.1Lag0013325.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity