Lag0013076 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0013076 vs. NCBI nr
Match: XP_030479238.1 (uncharacterized protein LOC115696478 [Cannabis sativa])

HSP 1 Score: 241.1 bits (614), Expect = 1.4e-59
Identity = 132/344 (38.37%), Postives = 179/344 (52.03%), Query Frame = 0

Query: 1    MCANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSL 60
            + A FWWGS+G+ +K HW  W+ +C +K  GGLGFR + + NQAMLAKQ+WR++ NPNSL
Sbjct: 728  LMAQFWWGSMGKGSKIHWKNWRTLCSSKFFGGLGFRSLISHNQAMLAKQAWRVLSNPNSL 787

Query: 61   LFKVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWI 120
            L  +LK KY K  NFL+A +G  PS TW SL WGRDL  +G  WKVGNG  I   +DPWI
Sbjct: 788  LTSILKAKYFKLNNFLEAKLGHTPSYTWSSLIWGRDLLIRGLLWKVGNGCSIRTFQDPWI 847

Query: 121  AREGNYKPCL----VP---------------------------EILKGKNTPLGIKESRD 180
               G   PCL    +P                            +L     P+G  +  D
Sbjct: 848  --PGIKFPCLRSSDLPPADKVSFFIDNSGFWDRKKLQQYFDEYSVLTILKIPIGGLQKAD 907

Query: 181  EIIWGRDKRGLFSVKSAYKLALEQRKECEASSSDHSNQSKLWKCLWSLNVIPRAKACAWK 240
             +IW ++  G+FSVKSAY +A         SSSD S     WK LW+LNV P+ K  AW+
Sbjct: 908  SLIWNQENSGIFSVKSAYHIA--NNASLPPSSSDSSLPKAWWKTLWNLNVQPKVKNFAWR 967

Query: 241  ILNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTLFNL 300
            + + I+P   N+  R      +CSFC    E  +H   +C  + +IW  +  +S  L N 
Sbjct: 968  VYHHILPVGLNLFVRKTIPQPACSFCPNPTETVTHALLDCPRASKIWKASPLRSFYLSNR 1027

Query: 301  RRDGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTRIHQN 314
              D     D+     E   K+  +  V T+W IW +RN ++  N
Sbjct: 1028 HVD---VKDFMIIGFEQLHKDQLVLLVTTLWAIWYSRNKKLFAN 1064

BLAST of Lag0013076 vs. NCBI nr
Match: VVA38592.1 (PREDICTED: reverse mRNAase, partial [Prunus dulcis])

HSP 1 Score: 240.4 bits (612), Expect = 2.3e-59
Identity = 124/356 (34.83%), Postives = 185/356 (51.97%), Query Frame = 0

Query: 1   MCANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSL 60
           M A FWWG  GE  K HWMRW+++CK K  GG+GFR + AFN AMLAKQ WRL+ NP+SL
Sbjct: 437 MMARFWWGQQGENRKIHWMRWERLCKAKTEGGMGFRCLQAFNMAMLAKQGWRLVHNPHSL 496

Query: 61  LFKVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWI 120
             ++LK KY    NF +A +G  PS  W+S+   R + E G R+++G+G+ + I  D W+
Sbjct: 497 ASRLLKAKYFPQTNFWEATLGSRPSCVWKSIWTARKVLEMGSRFQIGDGKSVRIWGDKWV 556

Query: 121 AREGNYKPCLVP---------------------------------EILKGKNTPLGIKES 180
            R   +     P                                 +++     PL I+  
Sbjct: 557 PRPATFAVITSPLDGMENTKVSELICNEGSPQWDLQKLNNLFLPVDVVDIVRIPLSIRAP 616

Query: 181 RDEIIWGRDKRGLFSVKSAYKLALEQRK-ECEASSSDHSNQSKLWKCLWSLNVIPRAKAC 240
            D I+W  DK GLF+VKSAY++AL     + + SSS +S+ S LW+ +W+ NV  + K  
Sbjct: 617 PDRIVWNYDKHGLFTVKSAYRVALRVTSGDEDESSSSNSDTSMLWRHIWNANVPTKLKIF 676

Query: 241 AWKILNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTL 300
           AW++ +DI+P KAN+ K+G+D    C FC    E + H    C F+   W  ++   LT 
Sbjct: 677 AWRVAHDILPTKANLIKKGVDMQDMCMFCGDITESALHVLAMCPFAVATWNISL---LTR 736

Query: 301 FNLRRDGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTRIHQN-QKPDHHLI 322
              +    T  D+ + ++E T K  +   +  +W IW  RN  + Q  Q+  H ++
Sbjct: 737 HAHQVRCHTMKDWLREMLEGTSKAQRDHIITLLWAIWGNRNALLWQGVQRSPHDVV 789

BLAST of Lag0013076 vs. NCBI nr
Match: XP_030509046.1 (uncharacterized protein LOC115723707 [Cannabis sativa])

HSP 1 Score: 236.5 bits (602), Expect = 3.4e-58
Identity = 123/357 (34.45%), Postives = 182/357 (50.98%), Query Frame = 0

Query: 1   MCANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSL 60
           M ANFWWG+  + +K HW RW+ +CK+K  GG+GFR    FNQAMLAKQ+WRL  NPNSL
Sbjct: 534 MMANFWWGTNKDGSKIHWKRWKLLCKSKFEGGMGFRSFVDFNQAMLAKQAWRLFDNPNSL 593

Query: 61  LFKVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWI 120
           L ++LK +Y    +FL+A  G +PS+ W+ + WGR+L  KG R+K+GNG ++  G +PWI
Sbjct: 594 LGRLLKHRYFPQNSFLEAHKGHSPSLMWQGIHWGRELLLKGLRYKIGNGFHVRSGLNPWI 653

Query: 121 AREGNYKPCLVP-----------------------------EILKGKNTPLGIKESRDEI 180
                +KP                                 +I +    PL   +S D +
Sbjct: 654 PGHDEFKPISYTRDPSTPVSTYILDTMEWNLPQLHQDFAQIDIDRILTIPLSFYQSSDRL 713

Query: 181 IWGRDKRGLFSVKSAYKLALEQRKECEASSSDHSNQSKLWKCLWSLNVIPRAKACAWKIL 240
           IW  +  G +SVKS + LA    ++ + SSSD  +    WK  W L++ P+ K   WK++
Sbjct: 714 IWHHNNNGFYSVKSGFHLATSLSEKDQESSSD--DYKTWWKFFWKLHLPPKVKIFTWKVI 773

Query: 241 NDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTLFNLRR 300
            + +P    + KR +  ++ C+ C+   E   H    CKF+K IW NT      +     
Sbjct: 774 QNALPVTTALHKRKVIDSALCTRCKSAWESIGHALFSCKFAKSIWKNT---KFQIDTHHA 833

Query: 301 DGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTRIHQNQKPDHHLITSKILDF 329
            G    DY   +    DKE+    +  MW IWN RN  +H  ++ D   + S  L++
Sbjct: 834 MGMFNGDYLIHLASTMDKENFEALICVMWSIWNDRNKFLHGGKQRDSFTLASYALNY 885

BLAST of Lag0013076 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 233.4 bits (594), Expect = 2.8e-57
Identity = 118/339 (34.81%), Postives = 181/339 (53.39%), Query Frame = 0

Query: 3   ANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSLLF 62
           A FWWGS  E  K HW+ W  +   K  GG+GFR++  FN+A+LAKQ WR++ +PNS+L 
Sbjct: 616 ARFWWGSSKEDKKIHWVAWNSLYLPKCEGGMGFRDLELFNKALLAKQCWRILNHPNSMLS 675

Query: 63  KVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWIA- 122
           +VLKG+Y KD +F++A +  NPS  WRS+ WGRDL +KG RW++GNG  + I  D W+  
Sbjct: 676 RVLKGRYFKDCSFMEAKISGNPSYIWRSILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPN 735

Query: 123 ------------------------REGNYKPCLV-----PEILKG-KNTPLGIKESRDEI 182
                                    EG ++  +V     P+  KG  + P+G     D +
Sbjct: 736 QPTLKILSSPRLPLVSRVSSLVDHEEGGWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRL 795

Query: 183 IWGRDKRGLFSVKSAYKLALEQRKECEASSSDHSNQSKL-WKCLWSLNVIPRAKACAWKI 242
           IW  +K G++SV+S YK+AL      +A SS  S + +  W   W +++  + K   W++
Sbjct: 796 IWNYEKTGVYSVRSGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRL 855

Query: 243 LNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTLFNLR 302
             D +P   N+ KRG++  + C FC +  E S H F  CKF++ +W+N+    L+ F + 
Sbjct: 856 CLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKLSPFLIL 915

Query: 303 RDGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTR 310
           R+            E+  K D  +  + +W +WN RN R
Sbjct: 916 RESH----------ESLSKADFEELCVVIWGLWNQRNAR 944

BLAST of Lag0013076 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 233.4 bits (594), Expect = 2.8e-57
Identity = 120/343 (34.99%), Postives = 186/343 (54.23%), Query Frame = 0

Query: 3    ANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSLLF 62
            A FWWGS G+K   HW +W+K+ + K  GGLGFRE S FNQA++AKQ+WRL++ PNSL+ 
Sbjct: 858  AKFWWGSKGDKRGIHWRKWEKLSQAKIRGGLGFREFSCFNQALVAKQAWRLLQYPNSLVS 917

Query: 63   KVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWIAR 122
            +VL+ +Y ++ +FL A  G N S  WRS+ WGR + +KG RW++GNG+ I I  D W+ R
Sbjct: 918  RVLQARYFRNSSFLCAKAGANASYIWRSIMWGRQVIKKGMRWRIGNGKKIAIFSDNWLPR 977

Query: 123  EGNYKP---------CLVPEILKGKN---------------------TPLGIKESRDEII 182
               ++P          +V +++K  N                      PL  +++ DE++
Sbjct: 978  PETFRPIFPLSLPVSSVVADLIKADNQWDEIKLRQHFLDVDTAEILKIPLPAEKAEDEVL 1037

Query: 183  WGRDKRGLFSVKSAYKLALEQRKECEASSSDHSNQSKLWKCLWSLNVIPRAKACAWKILN 242
            W  DKRG +SVKS Y+LAL  +     S ++ S+  K W  LW+L +  + K   W+  N
Sbjct: 1038 WHYDKRGNYSVKSGYQLALRSKFPDSTSCTEASH--KYWSALWTLELPEKLKIFMWRASN 1097

Query: 243  DIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTLFNLRRD 302
            +++P+  N+ KR +    +C  C+   E  SH   ECK +++IWL +   +  L    +D
Sbjct: 1098 NLLPSAENLWKRKVVEEPTCKRCKLSVETISHALLECKAARKIWLQSPFSAPRLEANSQD 1157

Query: 303  GWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTRIHQNQK 316
             ++ +   Q + +   K D    V   W  W ARN  I   ++
Sbjct: 1158 IFSTL---QNMAKELRKSDLELMVALCWSAWYARNKCIFDGRE 1195

BLAST of Lag0013076 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.2e-29
Identity = 87/320 (27.19%), Postives = 139/320 (43.44%), Query Frame = 0

Query: 5   FWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSLLFKV 64
           F WGS  EK K H ++W KVC  K+ GGLG R   + N+A+++K  WRL++  NSL   V
Sbjct: 73  FLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLV 132

Query: 65  LKGKYS----KDGNFLKAPVGKNPSMTWRSLCWG-RDLFEKGYRWKVGNGRYIDIGEDPW 124
           L+ KY     +D  +L  P G + S TWRS+  G RD+   G  W  G+G+ I    D W
Sbjct: 133 LQKKYHVGEIRDSRWL-IPKG-SWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRW 192

Query: 125 IA------REGNYKPCLVPEIL-------------------KGKNTPLGIKE-------- 184
           ++       +   +P     ++                      NT L ++         
Sbjct: 193 VSGKPLLELDNGERPTDCDTVVAKDLWIPGRGWDFAKIDPYTTNNTRLELRAVVLDLVTG 252

Query: 185 SRDEIIWGRDKRGLFSVKSAYKLALEQRKECEASSSDHSNQSKLWKCLWSLNVIPRAKAC 244
           +RD + W   + G FSV+SAY++                N +  + CLW + V  R K  
Sbjct: 253 ARDRLSWKFSQDGQFSVRSAYEM-------LTVDEVPRPNMASFFNCLWKVRVPERVKTF 312

Query: 245 AWKILNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTL 287
            W + N  V  +    +R +  ++ C  C+   E   H   +C     IW+  +P+    
Sbjct: 313 LWLVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVPQR--- 372

BLAST of Lag0013076 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.0e-25
Identity = 54/125 (43.20%), Postives = 78/125 (62.40%), Query Frame = 0

Query: 5   FWWGSLGEKTKSHWMRWQKVCKTKE-AGGLGFREVSAFNQAMLAKQSWRLIRNPNSLLFK 64
           FWW S   K K  W+ WQK+CK+KE  GGLGFR++  FNQA+LAKQS+R+I  P++LL +
Sbjct: 28  FWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQSFRIIHQPHTLLSR 87

Query: 65  VLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWIARE 124
           +L+ +Y    + ++  VG  PS  WRS+  GR+L  +G    +G+G +  +  D WI  E
Sbjct: 88  LLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGIHTKVWLDRWIMDE 147

Query: 125 GNYKP 129
               P
Sbjct: 148 TPLPP 152

BLAST of Lag0013076 vs. ExPASy TrEMBL
Match: A0A5E4GGB8 (PREDICTED: reverse mRNAase (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2B018728 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.1e-59
Identity = 124/356 (34.83%), Postives = 185/356 (51.97%), Query Frame = 0

Query: 1   MCANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSL 60
           M A FWWG  GE  K HWMRW+++CK K  GG+GFR + AFN AMLAKQ WRL+ NP+SL
Sbjct: 437 MMARFWWGQQGENRKIHWMRWERLCKAKTEGGMGFRCLQAFNMAMLAKQGWRLVHNPHSL 496

Query: 61  LFKVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWI 120
             ++LK KY    NF +A +G  PS  W+S+   R + E G R+++G+G+ + I  D W+
Sbjct: 497 ASRLLKAKYFPQTNFWEATLGSRPSCVWKSIWTARKVLEMGSRFQIGDGKSVRIWGDKWV 556

Query: 121 AREGNYKPCLVP---------------------------------EILKGKNTPLGIKES 180
            R   +     P                                 +++     PL I+  
Sbjct: 557 PRPATFAVITSPLDGMENTKVSELICNEGSPQWDLQKLNNLFLPVDVVDIVRIPLSIRAP 616

Query: 181 RDEIIWGRDKRGLFSVKSAYKLALEQRK-ECEASSSDHSNQSKLWKCLWSLNVIPRAKAC 240
            D I+W  DK GLF+VKSAY++AL     + + SSS +S+ S LW+ +W+ NV  + K  
Sbjct: 617 PDRIVWNYDKHGLFTVKSAYRVALRVTSGDEDESSSSNSDTSMLWRHIWNANVPTKLKIF 676

Query: 241 AWKILNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTL 300
           AW++ +DI+P KAN+ K+G+D    C FC    E + H    C F+   W  ++   LT 
Sbjct: 677 AWRVAHDILPTKANLIKKGVDMQDMCMFCGDITESALHVLAMCPFAVATWNISL---LTR 736

Query: 301 FNLRRDGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTRIHQN-QKPDHHLI 322
              +    T  D+ + ++E T K  +   +  +W IW  RN  + Q  Q+  H ++
Sbjct: 737 HAHQVRCHTMKDWLREMLEGTSKAQRDHIITLLWAIWGNRNALLWQGVQRSPHDVV 789

BLAST of Lag0013076 vs. ExPASy TrEMBL
Match: A0A803Q6Y1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 1.6e-58
Identity = 123/357 (34.45%), Postives = 182/357 (50.98%), Query Frame = 0

Query: 1    MCANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSL 60
            M ANFWWG+  + +K HW RW+ +CK+K  GG+GFR    FNQAMLAKQ+WRL  NPNSL
Sbjct: 1070 MMANFWWGTNKDGSKIHWKRWKLLCKSKFEGGMGFRSFVDFNQAMLAKQAWRLFDNPNSL 1129

Query: 61   LFKVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWI 120
            L ++LK +Y    +FL+A  G +PS+ W+ + WGR+L  KG R+K+GNG ++  G +PWI
Sbjct: 1130 LGRLLKHRYFPQNSFLEAHKGHSPSLMWQGIHWGRELLLKGLRYKIGNGFHVRSGLNPWI 1189

Query: 121  AREGNYKPCLVP-----------------------------EILKGKNTPLGIKESRDEI 180
                 +KP                                 +I +    PL   +S D +
Sbjct: 1190 PGHDEFKPISYTRDPSTPVSTYILDTMEWNLPQLHQDFAQIDIDRILTIPLSFYQSSDRL 1249

Query: 181  IWGRDKRGLFSVKSAYKLALEQRKECEASSSDHSNQSKLWKCLWSLNVIPRAKACAWKIL 240
            IW  +  G +SVKS + LA    ++ + SSSD  +    WK  W L++ P+ K   WK++
Sbjct: 1250 IWHHNNNGFYSVKSGFHLATSLSEKDQESSSD--DYKTWWKFFWKLHLPPKVKIFTWKVI 1309

Query: 241  NDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTLFNLRR 300
             + +P    + KR +  ++ C+ C+   E   H    CKF+K IW NT      +     
Sbjct: 1310 QNALPVTTALHKRKVIDSALCTRCKSAWESIGHALFSCKFAKSIWKNT---KFQIDTHHA 1369

Query: 301  DGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTRIHQNQKPDHHLITSKILDF 329
             G    DY   +    DKE+    +  MW IWN RN  +H  ++ D   + S  L++
Sbjct: 1370 MGMFNGDYLIHLASTMDKENFEALICVMWSIWNDRNKFLHGGKQRDSFTLASYALNY 1421

BLAST of Lag0013076 vs. ExPASy TrEMBL
Match: A0A803QI38 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 3.6e-58
Identity = 130/345 (37.68%), Postives = 178/345 (51.59%), Query Frame = 0

Query: 1    MCANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSL 60
            + A FWWGS+G+ +K HW  W+ +C +K  GGLGF+ + + NQAMLAKQ+WR++ NP SL
Sbjct: 1237 LMAQFWWGSMGKGSKIHWKNWRSLCSSKFFGGLGFKSLISHNQAMLAKQAWRVLSNPTSL 1296

Query: 61   LFKVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWI 120
            L  +LK KY K  NFL+A +G  PS TW SL WGRDL  +G  WKVGNG  I   +DPWI
Sbjct: 1297 LACILKAKYFKLNNFLEAKLGHTPSYTWSSLIWGRDLLVRGLLWKVGNGCSIRTFQDPWI 1356

Query: 121  -------AREGNYKP-------------------------CLVPEILKGKNTPLGIKESR 180
                    R  +  P                           V  ILK    P+G  +  
Sbjct: 1357 PGMKYPSLRSSDQPPDDKVSFFIDNSGFWDREKLQQYFDEYSVSTILK---IPIGGPQKT 1416

Query: 181  DEIIWGRDKRGLFSVKSAYKLALEQRKECEASSSDHSNQSKLWKCLWSLNVIPRAKACAW 240
            D +IW +D  G+FSVKSAY +A         SSSD S     WK LW+LNV P+ K  AW
Sbjct: 1417 DSLIWTQDNSGIFSVKSAYHIA--NNTSMPPSSSDSSLSKTWWKTLWNLNVQPKIKNFAW 1476

Query: 241  KILNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTLFN 300
            ++ + I+P   N+  R   +  +CSFC    E  +H   +C  + +IW  +  +S  L N
Sbjct: 1477 RVYHHILPVGLNLFVRKTISQPTCSFCPNPTETVTHALLDCPRASKIWKASPLRSFYLSN 1536

Query: 301  LRRDGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTRIHQN 314
               D     ++     E   K+  +  V T+W IW +RN ++  N
Sbjct: 1537 RHVD---VKEFMIIGFEQLHKDQLVLLVTTLWAIWYSRNKKLFAN 1573

BLAST of Lag0013076 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.4e-57
Identity = 118/339 (34.81%), Postives = 181/339 (53.39%), Query Frame = 0

Query: 3   ANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSLLF 62
           A FWWGS  E  K HW+ W  +   K  GG+GFR++  FN+A+LAKQ WR++ +PNS+L 
Sbjct: 616 ARFWWGSSKEDKKIHWVAWNSLYLPKCEGGMGFRDLELFNKALLAKQCWRILNHPNSMLS 675

Query: 63  KVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWIA- 122
           +VLKG+Y KD +F++A +  NPS  WRS+ WGRDL +KG RW++GNG  + I  D W+  
Sbjct: 676 RVLKGRYFKDCSFMEAKISGNPSYIWRSILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPN 735

Query: 123 ------------------------REGNYKPCLV-----PEILKG-KNTPLGIKESRDEI 182
                                    EG ++  +V     P+  KG  + P+G     D +
Sbjct: 736 QPTLKILSSPRLPLVSRVSSLVDHEEGGWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRL 795

Query: 183 IWGRDKRGLFSVKSAYKLALEQRKECEASSSDHSNQSKL-WKCLWSLNVIPRAKACAWKI 242
           IW  +K G++SV+S YK+AL      +A SS  S + +  W   W +++  + K   W++
Sbjct: 796 IWNYEKTGVYSVRSGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRL 855

Query: 243 LNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTLFNLR 302
             D +P   N+ KRG++  + C FC +  E S H F  CKF++ +W+N+    L+ F + 
Sbjct: 856 CLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKLSPFLIL 915

Query: 303 RDGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTR 310
           R+            E+  K D  +  + +W +WN RN R
Sbjct: 916 RESH----------ESLSKADFEELCVVIWGLWNQRNAR 944

BLAST of Lag0013076 vs. ExPASy TrEMBL
Match: A0A803QQH9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.4e-57
Identity = 134/361 (37.12%), Postives = 187/361 (51.80%), Query Frame = 0

Query: 1    MCANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSL 60
            M ANFWWG+    TK HW RW  +CK+K  GG+GFR    FNQA+LAKQ+WR+   P+SL
Sbjct: 1081 MMANFWWGTNQNGTKIHWKRWNSLCKSKHEGGMGFRSFIHFNQALLAKQAWRIFNMPDSL 1140

Query: 61   LFKVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWI 120
            L ++LK +Y    +FL A +G +PS TW+S+CWGR+L  KG R+KVGNG +I  G DPWI
Sbjct: 1141 LSRLLKHRYFSTTSFLDANIGHSPSYTWQSICWGRELLVKGMRFKVGNGHHITCGTDPWI 1200

Query: 121  AREGNYKP--------CLVPEILKGKNT---------------------PLGIKESRDEI 180
                NYKP         LV  ++  +                       PL    S D +
Sbjct: 1201 PGHTNYKPISYHGPPNLLVSSLITDQRVWNLELLNSFFQPIDVEKILTIPLSFFASNDRL 1260

Query: 181  IWGRDKRGLFSVKSAYKLA--LEQRKECEASSSDHSNQSKLWKCLWSLNVIPRAKACAWK 240
            IW     G ++VKS + LA  LE++  C AS S+ +     WK  WSLN+ P+ +  AWK
Sbjct: 1261 IWHHSTSGSYNVKSGFHLASSLEEQNTCSASDSNVA----WWKFFWSLNLPPKIRIFAWK 1320

Query: 241  ILNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIWLNTIPKSLTLFNL 300
            + ++I+P    + KR I  +++CS C    E   H   +C+ ++ IW ++  K L  FN+
Sbjct: 1321 VHHNILPTATALYKRKIIDSAACSLCTSSWESVGHALFDCRHARNIWRDS--KFLIDFNM 1380

Query: 301  RRDGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTRIHQNQKPD--HHLITSKILD 329
             +      DY Q++    +KED    V          +TRI   QK    H   TS   D
Sbjct: 1381 AKT-MQNGDYLQYLSSVYNKEDFELLV----------STRITAKQKKHSVHTTATSDSTD 1424

BLAST of Lag0013076 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 133.3 bits (334), Expect = 3.7e-31
Identity = 88/349 (25.21%), Postives = 147/349 (42.12%), Query Frame = 0

Query: 3   ANFWWGSLGEKTKSHWMRWQKVCKTKEAGGLGFREVSAFNQAMLAKQSWRLIRNPNSLLF 62
           A+FWW +  E    HW  W  +   K  GG+GF+++ AFN A+L KQ WR++  P SL+ 
Sbjct: 26  ADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIEAFNLALLGKQMWRMLSRPESLMA 85

Query: 63  KVLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWI-- 122
           KV K +Y    + L AP+G  PS  W+S+   +++  +G R  VGNG  I I    W+  
Sbjct: 86  KVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEILRQGARAVVGNGEDIIIWRHKWLDS 145

Query: 123 ------AREGNYKP-----------------------------CLVPEI---LKGKNTPL 182
                  R     P                              L PE+   L G+  P 
Sbjct: 146 KPASAALRMQRVPPQEYASVSSILKVSDLIDESGREWRKDVIEMLFPEVERKLIGELRP- 205

Query: 183 GIKESRDEIIWGRDKRGLFSVKSAYKLALE-QRKECEASSSDHSNQSKLWKCLWSLNVIP 242
           G +   D   W     G ++VKS Y +  +   K          + + +++ +W     P
Sbjct: 206 GGRRILDSYTWDYTSSGDYTVKSGYWVLTQIINKRSSPQEVSEPSLNPIYQKIWKSQTSP 265

Query: 243 RAKACAWKILNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSKRIW-LNTI 302
           + +   WK L++ +P    +  R +   S+C  C   +E  +H   +C F++  W +++I
Sbjct: 266 KIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCPSCKETVNHLLFKCTFARLTWAISSI 325

Query: 303 PKSLTLFNLRRDGWTPMDYWQWIVENTDKEDQIKAVITMWCIWNARNTR 310
           P  + L     D      YW + + N + + +  + +  W +W     R
Sbjct: 326 P--IPLGGEWADSIYVNLYWVFNLGNGNPQWEKASQLVPWLLWRLWKNR 371

BLAST of Lag0013076 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 119.0 bits (297), Expect = 7.3e-27
Identity = 54/125 (43.20%), Postives = 78/125 (62.40%), Query Frame = 0

Query: 5   FWWGSLGEKTKSHWMRWQKVCKTKE-AGGLGFREVSAFNQAMLAKQSWRLIRNPNSLLFK 64
           FWW S   K K  W+ WQK+CK+KE  GGLGFR++  FNQA+LAKQS+R+I  P++LL +
Sbjct: 28  FWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQSFRIIHQPHTLLSR 87

Query: 65  VLKGKYSKDGNFLKAPVGKNPSMTWRSLCWGRDLFEKGYRWKVGNGRYIDIGEDPWIARE 124
           +L+ +Y    + ++  VG  PS  WRS+  GR+L  +G    +G+G +  +  D WI  E
Sbjct: 88  LLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGIHTKVWLDRWIMDE 147

Query: 125 GNYKP 129
               P
Sbjct: 148 TPLPP 152

BLAST of Lag0013076 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 44.3 bits (103), Expect = 2.3e-04
Identity = 21/61 (34.43%), Postives = 31/61 (50.82%), Query Frame = 0

Query: 194 LWSLNVIPRAKACAWKILNDIVPNKANIKKRGIDTNSSCSFCRKKEEFSSHFFCECKFSK 253
           +WSL + P+ K   WK LN+ +P  A +  R I     C+ CR  E   +H    C F++
Sbjct: 9   IWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRCRDFETI-THILFNCPFAQ 68

Query: 254 R 255
           R
Sbjct: 69  R 68