Lag0012954 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012954
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr1: 46083470 .. 46084372 (-)
RNA-Seq ExpressionLag0012954
SyntenyLag0012954
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATAATCTCAGGTATCACCATGTTCTTGTTAACGGAAATTGTCCTGTTTGTCAGGAAGAGCTGGAGACCACAGATCATGCTCTATTCCTTTGCAAGAGAGCCAGGGAGGTTTGAGATATTCTTTTTCCATCCTTGCTTGGCAATTCTGAATTGTTGGACATTAAAGAGCGTTGGTTGCTTTTTAGCGAGTTTTAGGGGTTGGATATTGAAAGGATTTGTGTCGCAGCATGGTCCATTTGGAACGATAGGAATACTTGGATCCATAACCATCCCATTCCTACGGCTACAGGGAGATGTGAATGGATTGATGATTATATATCCGAATATAGGACGACTAATCCCAAGGGTGGGGCTCTTGTACAGTCAGAGAAGGATGTTACCAGGATTATCTCAATGAGTGAAGAGATTATTTTAAATACCGATGTAGCGTATATGGGAGCTATTGGTAAAAGTGGAATTGGGTTAATCATGCGGGATAGATCGGGACAAATAATCCAGGCGGCTCAGGCAATGATCTCTCCAGTTTCTACTTCAGCGTTAGGGGCGGAAGCGGAGGCAGTGTTACAAGGGCTTCGACTAGCCAGATCAATGGGCATTAGTCGGTTAACAGTTATGTCTGATTCTCTGAATCTGGTTAAATCGATTAATAAGGAGATTAATCAAGGCGATTCGTGCATTACTTCCACTATGTGGGATATTCATGATATACGATCAAACTTCACCAAGGTGACCTTTAACTTTGTTAGTAGGAGATTCAACGACATTGCCCACTCATTGGCAAATGCAGGTCTTTATTCACCTTCCGTATTATGGTTGGATAACTATCCGGAATGGATAGAAAATTTGTCGTCACAAGAACGACGTCTGTTTGTATCCCTTCAGGGGCATTCTGATTATTAA

mRNA sequence

ATGAATAATCTCAGGTATCACCATGTTCTTGTTAACGGAAATTGTCCTGTTTGTCAGGAAGAGCTGGAGACCACAGATCATGCTCTATTCCTTTGCAAGAGAGCCAGGGAGGGGTTGGATATTGAAAGGATTTGTGTCGCAGCATGGTCCATTTGGAACGATAGGAATACTTGGATCCATAACCATCCCATTCCTACGGCTACAGGGAGATGTGAATGGATTGATGATTATATATCCGAATATAGGACGACTAATCCCAAGGGTGGGGCTCTTGTACAGTCAGAGAAGGATGTTACCAGGATTATCTCAATGAGTGAAGAGATTATTTTAAATACCGATGTAGCGTATATGGGAGCTATTGGTAAAAGTGGAATTGGGTTAATCATGCGGGATAGATCGGGACAAATAATCCAGGCGGCTCAGGCAATGATCTCTCCAGTTTCTACTTCAGCGTTAGGGGCGGAAGCGGAGGCAGTGTTACAAGGGCTTCGACTAGCCAGATCAATGGGCATTAGTCGGTTAACAGTTATGTCTGATTCTCTGAATCTGGTTAAATCGATTAATAAGGAGATTAATCAAGGCGATTCGTGCATTACTTCCACTATGTGGGATATTCATGATATACGATCAAACTTCACCAAGGTGACCTTTAACTTTGTTAGTAGGAGATTCAACGACATTGCCCACTCATTGGCAAATGCAGGTCTTTATTCACCTTCCGTATTATGGTTGGATAACTATCCGGAATGGATAGAAAATTTGTCGTCACAAGAACGACGTCTGTTTGTATCCCTTCAGGGGCATTCTGATTATTAA

Coding sequence (CDS)

ATGAATAATCTCAGGTATCACCATGTTCTTGTTAACGGAAATTGTCCTGTTTGTCAGGAAGAGCTGGAGACCACAGATCATGCTCTATTCCTTTGCAAGAGAGCCAGGGAGGGGTTGGATATTGAAAGGATTTGTGTCGCAGCATGGTCCATTTGGAACGATAGGAATACTTGGATCCATAACCATCCCATTCCTACGGCTACAGGGAGATGTGAATGGATTGATGATTATATATCCGAATATAGGACGACTAATCCCAAGGGTGGGGCTCTTGTACAGTCAGAGAAGGATGTTACCAGGATTATCTCAATGAGTGAAGAGATTATTTTAAATACCGATGTAGCGTATATGGGAGCTATTGGTAAAAGTGGAATTGGGTTAATCATGCGGGATAGATCGGGACAAATAATCCAGGCGGCTCAGGCAATGATCTCTCCAGTTTCTACTTCAGCGTTAGGGGCGGAAGCGGAGGCAGTGTTACAAGGGCTTCGACTAGCCAGATCAATGGGCATTAGTCGGTTAACAGTTATGTCTGATTCTCTGAATCTGGTTAAATCGATTAATAAGGAGATTAATCAAGGCGATTCGTGCATTACTTCCACTATGTGGGATATTCATGATATACGATCAAACTTCACCAAGGTGACCTTTAACTTTGTTAGTAGGAGATTCAACGACATTGCCCACTCATTGGCAAATGCAGGTCTTTATTCACCTTCCGTATTATGGTTGGATAACTATCCGGAATGGATAGAAAATTTGTCGTCACAAGAACGACGTCTGTTTGTATCCCTTCAGGGGCATTCTGATTATTAA

Protein sequence

MNNLRYHHVLVNGNCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRNTWIHNHPIPTATGRCEWIDDYISEYRTTNPKGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQERRLFVSLQGHSDY
Homology
BLAST of Lag0012954 vs. NCBI nr
Match: GAU33827.1 (hypothetical protein TSUD_221650 [Trifolium subterraneum])

HSP 1 Score: 103.6 bits (257), Expect = 2.7e-18
Identity = 82/255 (32.16%), Postives = 124/255 (48.63%), Query Frame = 0

Query: 14   NCPVCQEELETTDHALFLCKRAREGLD-IERICVAAWSIWNDRN--TWIHNHPIPTATGR 73
            NCPVC EE+E   H  F    + E  D + R+ +  W IW++RN   W  N  +P   GR
Sbjct: 1100 NCPVCDEEIEDELHIFF----SNESSDTVGRVAMLLWCIWHNRNDKLWNDNVQLPRQIGR 1159

Query: 74   ---CEWIDDY-ISEYRTTNPKGGA---LVQSEKDVTRIISMSEEIILNTDVAYMGAIGKS 133
                 W D Y + + ++ N  G     LV+ EK         + +  N DVA++   G++
Sbjct: 1160 HALDAWNDWYSVHKLQSNNVSGSIKTDLVRWEKPTL------DWVKCNVDVAFVSGSGRT 1219

Query: 134  GIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNL 193
             +GL  RD SGQ + A          S++  EA A+L  +  AR  G+ R+   SDS  L
Sbjct: 1220 SVGLCFRDNSGQFM-AGMTQWQQTVISSVEEEAWALLLAMEEARHRGLDRVQFESDSKVL 1279

Query: 194  VKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLW 253
            +++I+ +  +G+S   S + DI  + S+F      FV R+ N +AH+LA A     S   
Sbjct: 1280 IEAIHMK-RRGNSEFLSIVHDILSLMSSFINFEVKFVRRQANLVAHTLARAANSWASFHR 1339

Query: 254  LDNYPEWIENLSSQE 259
             +N P  IE+L   E
Sbjct: 1340 FENIPFCIEHLIFNE 1342

BLAST of Lag0012954 vs. NCBI nr
Match: GAU40431.1 (hypothetical protein TSUD_397500 [Trifolium subterraneum])

HSP 1 Score: 103.2 bits (256), Expect = 3.6e-18
Identity = 81/254 (31.89%), Postives = 117/254 (46.06%), Query Frame = 0

Query: 14   NCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRN--TWIHNHPIPTATGRC 73
            NCPVC EE+E   H  F  K +     + R+ +  W IW++RN   W  N  +P+  GR 
Sbjct: 793  NCPVCDEEIEDEIHIFFSNKSSD---TVGRVAMLLWCIWHNRNDKLWNDNIQMPSQIGRY 852

Query: 74   ---EWIDDYISEYRTTNPKGGA----LVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSG 133
                W D Y      +N   G     LV+ EK     +        N DVA++   G++ 
Sbjct: 853  AFDAWNDWYSVHKLQSNSVSGTTEVDLVRWEKPALGWVK------YNVDVAFVSGSGRTS 912

Query: 134  IGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLV 193
            +GL  RD SGQ + A          S++  EA A+L  +  AR  G+ R+   SDS  L+
Sbjct: 913  VGLCFRDSSGQFM-AGMTQWQQTVISSVEGEAWALLLAMEEARHRGMDRVQFESDSKVLI 972

Query: 194  KSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWL 253
              I+ +  +G+S   S + DI D  S+F      FV R+ N +AH+LA A          
Sbjct: 973  DVIHMK-RRGNSEFLSIVHDILDFMSSFLNFEVKFVKRQANSVAHTLARAANSWSCFHRF 1032

Query: 254  DNYPEWIENLSSQE 259
            +N P  IE+L   E
Sbjct: 1033 ENIPLCIEHLVFNE 1035

BLAST of Lag0012954 vs. NCBI nr
Match: GAU47930.1 (hypothetical protein TSUD_14000 [Trifolium subterraneum])

HSP 1 Score: 99.0 bits (245), Expect = 6.7e-17
Identity = 86/260 (33.08%), Postives = 120/260 (46.15%), Query Frame = 0

Query: 12  NGNCPVCQEELETTDHALFLCKRAREGLDI-ERICVAAWSIWNDRN--TWIHNHPIPTAT 71
           N NCPVC EE++   H  F    + E  DI  R+ +  W IW +RN   W  N   P   
Sbjct: 142 NLNCPVCDEEIKDEIHIFF----SNESSDIVGRMVMLLWCIWQNRNDKLWNDNVQTPRQI 201

Query: 72  GRC---EWIDDYI-------SEYRTTNPKGGALVQSEKDVTRIISMSEEIILNTDVAYMG 131
           GR     W D Y        SE RTT      LV+ EK     +        N DVA++ 
Sbjct: 202 GRYVFDAWNDWYSVHKLQSDSESRTTE---ADLVRWEKPALGWVK------CNVDVAFVP 261

Query: 132 AIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMS 191
             G++ +GL  R+  GQ++         V +S  G EA A+L  +  AR  G+ R+   S
Sbjct: 262 GSGRTSVGLCFRNNRGQVMAGMTHWQQTVMSSVEG-EAWALLLAMEEARHRGLDRVQFES 321

Query: 192 DSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYS 251
           DS  LV++I+ +  +G+S   S + DI    S+F      FV R+ N +AH+LA A    
Sbjct: 322 DSKVLVEAIHMK-RRGNSEFLSIVHDIISFMSSFLNFEVKFVRRQANSVAHTLARAANSW 381

Query: 252 PSVLWLDNYPEWIENLSSQE 259
            S    +N P  IE+L   E
Sbjct: 382 SSFHRFENIPLCIEHLVVNE 386

BLAST of Lag0012954 vs. NCBI nr
Match: GAU36566.1 (hypothetical protein TSUD_277720 [Trifolium subterraneum])

HSP 1 Score: 99.0 bits (245), Expect = 6.7e-17
Identity = 84/284 (29.58%), Postives = 126/284 (44.37%), Query Frame = 0

Query: 14  NCPVCQEELETTDHALFLCKRARE-----GLD---------------------------- 73
           NCPVC EE+E   H  F C  AR+     GL                             
Sbjct: 558 NCPVCDEEIEDELHIFFRCAVARDSWSAAGLSFVLHNATYQQTNAMDRIFAVCNNESSDT 617

Query: 74  IERICVAAWSIWNDRN--TWIHNHPIPTATGR---CEWIDDYISEYRTTNPKGGALVQSE 133
           + R+ +  WSIW++RN   W  N  +P  TGR     W D Y      +N        +E
Sbjct: 618 VGRVAMLLWSIWHNRNDKLWNDNVQMPRQTGRHAFDAWNDWYSVHKLQSN---NVSRTTE 677

Query: 134 KDVTRIISMS-EEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGA 193
           +D+ R    + + +  N D A++   G++ +GL  RD SG  + A          S++  
Sbjct: 678 EDLVRWEKPALDWVKCNVDAAFVSGSGRTSVGLCFRDNSGHFM-AGMTQWQQTVISSVEG 737

Query: 194 EAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTK 253
           EA A+L  +  AR  G+ R+   SDS  L+++I+ +  +G+S   S + DI  + S+F  
Sbjct: 738 EAWALLLAMEEARHRGLDRVQFESDSKVLIEAIHMQ-RRGNSEFLSIVHDILSLMSSFIN 797

Query: 254 VTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE 259
              NFV R+ N +AH+LA A     S    +N P  IE+L   E
Sbjct: 798 FEVNFVRRQANLVAHTLARAANSWASFHRFENIPFCIEHLIFNE 836

BLAST of Lag0012954 vs. NCBI nr
Match: GAU41000.1 (hypothetical protein TSUD_92250 [Trifolium subterraneum])

HSP 1 Score: 98.6 bits (244), Expect = 8.8e-17
Identity = 90/292 (30.82%), Postives = 124/292 (42.47%), Query Frame = 0

Query: 12  NGNCPVCQEELETTDHALFLCKRARE-----GLD-------------------------- 71
           N NCPVC EE+E   H  F C  AR+     GL                           
Sbjct: 493 NLNCPVCDEEIEYEIHIFFRCAVARDSWCAAGLSSVLHNDAYQQSNAMNRIFAVCSNDSS 552

Query: 72  --IERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYI-------SEYRTTNP 131
             + R+ +  W IW +RN   WI N   P   GR     W D Y        SE RTT  
Sbjct: 553 DIVGRVAMLLWCIWQNRNDKLWIDNVQTPRQIGRYAFDAWNDWYSIHKLQSNSESRTTE- 612

Query: 132 KGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISP 191
               LV+ EK     +        N DVA++   G++ +GL  R+  GQ++         
Sbjct: 613 --ADLVRWEKPALGWVK------CNVDVAFVSGSGRTSVGLCFRNNRGQVMAGITQWQQT 672

Query: 192 VSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIH 251
           V +S  G EA A+L  +  AR  G+ R+   SDS  LV++I+ +  +G+S   S + DI 
Sbjct: 673 VMSSIEG-EAWALLLAMEEARHRGLDRVQFESDSKVLVEAIHMK-RRGNSEFLSIVHDII 732

Query: 252 DIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE 259
              S+F      FV R+ N +AH+LA A     S    +N P  IE+L   E
Sbjct: 733 TFMSSFLNFEVKFVRRQTNSVAHTLARAANSWSSFHRFENIPLCIEHLVVNE 773

BLAST of Lag0012954 vs. ExPASy TrEMBL
Match: A0A2Z6NUW6 (CCHC-type domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_221650 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.3e-18
Identity = 82/255 (32.16%), Postives = 124/255 (48.63%), Query Frame = 0

Query: 14   NCPVCQEELETTDHALFLCKRAREGLD-IERICVAAWSIWNDRN--TWIHNHPIPTATGR 73
            NCPVC EE+E   H  F    + E  D + R+ +  W IW++RN   W  N  +P   GR
Sbjct: 1100 NCPVCDEEIEDELHIFF----SNESSDTVGRVAMLLWCIWHNRNDKLWNDNVQLPRQIGR 1159

Query: 74   ---CEWIDDY-ISEYRTTNPKGGA---LVQSEKDVTRIISMSEEIILNTDVAYMGAIGKS 133
                 W D Y + + ++ N  G     LV+ EK         + +  N DVA++   G++
Sbjct: 1160 HALDAWNDWYSVHKLQSNNVSGSIKTDLVRWEKPTL------DWVKCNVDVAFVSGSGRT 1219

Query: 134  GIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNL 193
             +GL  RD SGQ + A          S++  EA A+L  +  AR  G+ R+   SDS  L
Sbjct: 1220 SVGLCFRDNSGQFM-AGMTQWQQTVISSVEEEAWALLLAMEEARHRGLDRVQFESDSKVL 1279

Query: 194  VKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLW 253
            +++I+ +  +G+S   S + DI  + S+F      FV R+ N +AH+LA A     S   
Sbjct: 1280 IEAIHMK-RRGNSEFLSIVHDILSLMSSFINFEVKFVRRQANLVAHTLARAANSWASFHR 1339

Query: 254  LDNYPEWIENLSSQE 259
             +N P  IE+L   E
Sbjct: 1340 FENIPFCIEHLIFNE 1342

BLAST of Lag0012954 vs. ExPASy TrEMBL
Match: A0A2Z6N9N6 (ZnF_PMZ domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_397500 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.7e-18
Identity = 81/254 (31.89%), Postives = 117/254 (46.06%), Query Frame = 0

Query: 14   NCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRN--TWIHNHPIPTATGRC 73
            NCPVC EE+E   H  F  K +     + R+ +  W IW++RN   W  N  +P+  GR 
Sbjct: 793  NCPVCDEEIEDEIHIFFSNKSSD---TVGRVAMLLWCIWHNRNDKLWNDNIQMPSQIGRY 852

Query: 74   ---EWIDDYISEYRTTNPKGGA----LVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSG 133
                W D Y      +N   G     LV+ EK     +        N DVA++   G++ 
Sbjct: 853  AFDAWNDWYSVHKLQSNSVSGTTEVDLVRWEKPALGWVK------YNVDVAFVSGSGRTS 912

Query: 134  IGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLV 193
            +GL  RD SGQ + A          S++  EA A+L  +  AR  G+ R+   SDS  L+
Sbjct: 913  VGLCFRDSSGQFM-AGMTQWQQTVISSVEGEAWALLLAMEEARHRGMDRVQFESDSKVLI 972

Query: 194  KSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWL 253
              I+ +  +G+S   S + DI D  S+F      FV R+ N +AH+LA A          
Sbjct: 973  DVIHMK-RRGNSEFLSIVHDILDFMSSFLNFEVKFVKRQANSVAHTLARAANSWSCFHRF 1032

Query: 254  DNYPEWIENLSSQE 259
            +N P  IE+L   E
Sbjct: 1033 ENIPLCIEHLVFNE 1035

BLAST of Lag0012954 vs. ExPASy TrEMBL
Match: A0A2Z6PCM8 (Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_14000 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.3e-17
Identity = 86/260 (33.08%), Postives = 120/260 (46.15%), Query Frame = 0

Query: 12  NGNCPVCQEELETTDHALFLCKRAREGLDI-ERICVAAWSIWNDRN--TWIHNHPIPTAT 71
           N NCPVC EE++   H  F    + E  DI  R+ +  W IW +RN   W  N   P   
Sbjct: 142 NLNCPVCDEEIKDEIHIFF----SNESSDIVGRMVMLLWCIWQNRNDKLWNDNVQTPRQI 201

Query: 72  GRC---EWIDDYI-------SEYRTTNPKGGALVQSEKDVTRIISMSEEIILNTDVAYMG 131
           GR     W D Y        SE RTT      LV+ EK     +        N DVA++ 
Sbjct: 202 GRYVFDAWNDWYSVHKLQSDSESRTTE---ADLVRWEKPALGWVK------CNVDVAFVP 261

Query: 132 AIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMS 191
             G++ +GL  R+  GQ++         V +S  G EA A+L  +  AR  G+ R+   S
Sbjct: 262 GSGRTSVGLCFRNNRGQVMAGMTHWQQTVMSSVEG-EAWALLLAMEEARHRGLDRVQFES 321

Query: 192 DSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYS 251
           DS  LV++I+ +  +G+S   S + DI    S+F      FV R+ N +AH+LA A    
Sbjct: 322 DSKVLVEAIHMK-RRGNSEFLSIVHDIISFMSSFLNFEVKFVRRQANSVAHTLARAANSW 381

Query: 252 PSVLWLDNYPEWIENLSSQE 259
            S    +N P  IE+L   E
Sbjct: 382 SSFHRFENIPLCIEHLVVNE 386

BLAST of Lag0012954 vs. ExPASy TrEMBL
Match: A0A2Z6NW00 (Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_92250 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 4.3e-17
Identity = 90/292 (30.82%), Postives = 124/292 (42.47%), Query Frame = 0

Query: 12  NGNCPVCQEELETTDHALFLCKRARE-----GLD-------------------------- 71
           N NCPVC EE+E   H  F C  AR+     GL                           
Sbjct: 493 NLNCPVCDEEIEYEIHIFFRCAVARDSWCAAGLSSVLHNDAYQQSNAMNRIFAVCSNDSS 552

Query: 72  --IERICVAAWSIWNDRN--TWIHNHPIPTATGRC---EWIDDYI-------SEYRTTNP 131
             + R+ +  W IW +RN   WI N   P   GR     W D Y        SE RTT  
Sbjct: 553 DIVGRVAMLLWCIWQNRNDKLWIDNVQTPRQIGRYAFDAWNDWYSIHKLQSNSESRTTE- 612

Query: 132 KGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISP 191
               LV+ EK     +        N DVA++   G++ +GL  R+  GQ++         
Sbjct: 613 --ADLVRWEKPALGWVK------CNVDVAFVSGSGRTSVGLCFRNNRGQVMAGITQWQQT 672

Query: 192 VSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIH 251
           V +S  G EA A+L  +  AR  G+ R+   SDS  LV++I+ +  +G+S   S + DI 
Sbjct: 673 VMSSIEG-EAWALLLAMEEARHRGLDRVQFESDSKVLVEAIHMK-RRGNSEFLSIVHDII 732

Query: 252 DIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNYPEWIENLSSQE 259
              S+F      FV R+ N +AH+LA A     S    +N P  IE+L   E
Sbjct: 733 TFMSSFLNFEVKFVRRQTNSVAHTLARAANSWSSFHRFENIPLCIEHLVVNE 773

BLAST of Lag0012954 vs. ExPASy TrEMBL
Match: A0A2Z6P8I4 (Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_77650 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 7.3e-17
Identity = 78/251 (31.08%), Postives = 117/251 (46.61%), Query Frame = 0

Query: 14  NCPVCQEELETTDHALFLCKRAREGLDIERICVAAWSIWNDRN--TWIHNHPIPTATGR- 73
           NCPVC EE+E   H +F          + R+ +  W IW +RN   W  N  +P   GR 
Sbjct: 290 NCPVCDEEIEDELH-IFFSVHNESSDTVGRVAMLLWCIWKNRNDKLWNDNVHMPRQIGRH 349

Query: 74  --CEWIDDYISEYRTTNPKGGALVQSEKDVTRIISMS-EEIILNTDVAYMGAIGKSGIGL 133
               W D Y      +N   G    +E DV R    + + +  N DVA++   G++ +GL
Sbjct: 350 AFDAWNDWYSVHKLQSNNMSGT---TEADVVRWEKPALDWVKCNVDVAFVSGSGRTSMGL 409

Query: 134 IMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSI 193
             RD SG  + A          S++  EA A+L  +  AR  G++R+   SD   L+++I
Sbjct: 410 CFRDNSGHFM-AGMTQWQQTVISSVEGEAWALLLAMEEARHRGLNRVQFESDLKVLIEAI 469

Query: 194 NKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFNDIAHSLANAGLYSPSVLWLDNY 253
           + +  +G+S   S + DI  + S+F      FV R+ N +AH+LA A     S    +N 
Sbjct: 470 HVK-RRGNSEFLSIVHDILSLMSSFINFEVKFVRRQANLVAHTLARAANSWASFHRFENI 529

Query: 254 PEWIENLSSQE 259
           P  IE L   E
Sbjct: 530 PFCIERLVFNE 534

BLAST of Lag0012954 vs. TAIR 10
Match: AT4G09490.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-05
Identity = 40/137 (29.20%), Postives = 69/137 (50.36%), Query Frame = 0

Query: 106 EEIILNTDVAYMGAIGKSGIGLIMRDRSGQIIQAAQAMISPVSTSALGAEAEAVLQGLRL 165
           + + + TD A+       G G ++R+         Q+    V    L AEA A+   L+ 
Sbjct: 29  DRVTIFTDAAWKHETKDVGFGWVIRNCPELTALHYQSAARNVRL-PLMAEAIALFLALQY 88

Query: 166 ARSMGISRLTVMSDSLNLVKSINKEINQGDSCITSTMWDIHDIRSNFTKVTFNFVSRRFN 225
           A+S+GI++L++ SDS  L+ +I  E    +      ++DI ++   F  V+F+FV R  N
Sbjct: 89  AQSIGITKLSMASDSQQLITAITSESPSTE--FYGIIFDILNLSLGFADVSFSFVPRSEN 148

Query: 226 DIAHSLANAGLYSPSVL 243
            +A  LA + L S S++
Sbjct: 149 RVADELAKSSLISFSIV 162

BLAST of Lag0012954 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 6.3e-05
Identity = 68/298 (22.82%), Postives = 110/298 (36.91%), Query Frame = 0

Query: 11  VNGNCPVCQEELETTDHALFLCKRA---------------------REGL---------- 70
           ++ +CP C  E E+ +HALF C  A                      E +          
Sbjct: 196 IDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSNDFEENISNILNFVQDT 255

Query: 71  ---DIERICVA--AWSIWNDRNTWIHN--HPIPTAT------GRCEWIDDYISEYRTTNP 130
              D  ++      W IW  RN  + N     P+ T         +W++   S  +T +P
Sbjct: 256 TMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNATQSHKKTPSP 315

Query: 131 KGGALVQSEKDVTRIISMSEEIILNTDVAYMGAIGKSGI---------GLIMRDRSGQII 190
                       TR I+ ++    N    Y+     +G          G I+R+  G  I
Sbjct: 316 ------------TRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPI 375

Query: 191 QAAQAMISPVSTSALGAEAEAVLQGLRLARSMGISRLTVMSDSLNLVKSINKEINQGDSC 248
                 ++  S + L AE +A+L  L+     G +++ +  D   L+  IN       S 
Sbjct: 376 SWGSMKLAHTS-NPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLING--ISFHSS 435

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GAU33827.12.7e-1832.16hypothetical protein TSUD_221650 [Trifolium subterraneum][more]
GAU40431.13.6e-1831.89hypothetical protein TSUD_397500 [Trifolium subterraneum][more]
GAU47930.16.7e-1733.08hypothetical protein TSUD_14000 [Trifolium subterraneum][more]
GAU36566.16.7e-1729.58hypothetical protein TSUD_277720 [Trifolium subterraneum][more]
GAU41000.18.8e-1730.82hypothetical protein TSUD_92250 [Trifolium subterraneum][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A2Z6NUW61.3e-1832.16CCHC-type domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_22... [more]
A0A2Z6N9N61.7e-1831.89ZnF_PMZ domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_3975... [more]
A0A2Z6PCM83.3e-1733.08Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_14000 PE=4 SV=... [more]
A0A2Z6NW004.3e-1730.82Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_92250 PE=4 SV=... [more]
A0A2Z6P8I47.3e-1731.08Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_77650 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT4G09490.11.3e-0529.20Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G09510.16.3e-0522.82Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 111..234
e-value: 2.0E-23
score: 82.5
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 137..237
score: 9.051778
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 106..236
e-value: 2.7E-20
score: 74.7
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 110..257
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 110..257
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 110..233
e-value: 8.21629E-22
score: 85.8288
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 108..238

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012954.1Lag0012954.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity