Lag0012950 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012950
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCytochrome P450
Locationchr1: 46020997 .. 46023136 (+)
RNA-Seq ExpressionLag0012950
SyntenyLag0012950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCGGCGACTGGCGGCCACCGCTCGCAGCCGCCGTCGGCCGCCGGAGGCTGACGGCAGCGGCGAGGATGGCCGACGATGGTGGAGACAGGTGCGATGAGGGAGATCGACAATGTGGGTGGGAGTTTTAGGGTATTAGGGTTTTCTTTTTGTTTTCTTTTTTTTTTAATAAAAAAAACCCTTTGGGTCTACCCAGACGTGTCCAATCTCGTGTCCTAACGTGTCCGAGTGTGTCCATTTAAAAAAATAAAAATAAATAGGACACGCAAAATTGCGTGTTCGACACGTGTCCAATGCGTGTCCGGACATATCCGTGTCCGACACTGATTCTTCACCATATATAACGTGTTTGTGCTTCTTAGCAAGTTAGTATATAAAATAGTAGTACAGTTAGAAAGGTTACGATATAATTACATATAGGTTTGAATGAATCTACTTCCAAGGAAAAGTTTGGCTGCATAACGATATGTAGGGATCTCCTACATACCACCGTAAAAATCTTGACAACTAGGAGAGATAAAAAATGTTTTCTCATTTTCTATCTTTCTCTTTCGGGAGATCCCTACGTGTCGGTATGCAGCCAAGTATTGTTTTAATTACAATATATAGTATATTTAATGTTGTTTTATGCATATTATTTCTTAACTTATTTGCTTGGATACAAGTTAAATTATCATTTAACCCAAAAATCTAAAATGATAGGTTGCAGTTATAATTTTAATCTTTAAGCAAATTCGTTATATGACACACCATGTTTAAAATACTACTCAAACCCCATAGCTTTTTTTTTTTCTTTCTTTTTTTCTTTTTTTTTTTCCGAAATTTGTCCACCTAAAAAGTTAAAGCTATAAGCTAATAATAACTTAAGATTATTATTATTATTTTTTATAATATGGAGTTGTGAGTTTGAACCCTAAATTGTCATAAGTACAAGTTTTATGTCAATTGAGTTATACTGAAGTTGTTTGTAGCTTAATATTTTATTCAGTGTCTTTTACATTTGTTTAATGTGTGCTGATAGATGCTTAGAACTGCTTTAGAATAATTGTTGTGTTTAACTTTAAGTTTAAAAATGATAAAAAGTGCTTTTGACCCACCAAATTAAATTAATGTACTTTCTTTAAATTTAAGGCAACAACAATTGGTTTTAGAAAATCTACAACAATAAAAAAGCCCTTAATTCTTTTAAAGATCAACTAGTTTTATTTCAAAGGTACATATATAATAACTATTCCATCAAAAAATACTATTTTCCAAACACATGTTAACGCACTTTAATCATTTATTCACTATTCATATTAACCTAAATTACTATTCAATCCAAAAACTTTAAGTTGATTAGATACTTCGGTACGATACTACCTCTTAAATTATCATCAAGGTCTAGATCCAAAAGCCTTATCGTAATATATTTAATCTTTTATACATATTCTTCATAGGTATATAGTTAACGAATATAACGAGCTCGAATGCACCGACAAGTTCTTGAGAGACACCGTCTTAAACTTCATGATGGCGGGGCGAGACGCTTTCAGCGCAACTCTCTCATGGTTGTTCTTCATCCTCTCAAAGAATCCAATTGTGGTAGAAAAGATCCGAGAAGAGCTCAACGGCACAACAATCCCACAGAGCGAAGAATCTAAAAAACAACCACGAATTTTCACCATTGAAGAGCTCAACGACCTCGTTTACTTGCATGGAGCCTTATGCGAAACGCTTCGTCTCTATCCTCCGATTGCGTTCGAGCACAAATCTCCGGCAGAGGCCGACACCCTTCCGAGCGGCCACCACGTGAAGCCACACACGATGATCCTCTTCTCGTTGTACGCACTGGGAAGAATGAGGTCTGTTTGGGGAGAGGATTGCGAAGAATTCAAACCAGAGAGATGGGTTTCGGAGAAGGGGACGATCAAACGAGAGCCTTCCTATAAGTTCTTCAGTTTCAATGCAGGGCCGAGGAGTTGTTTGGGAAAAGGAGTGGCTTTTTCCCAGTTGAAGATTGTTTCAGCTGCCATTATCCATAACTACGACATTCAAGCTGTTGAAGACAACAACAATGTTGTTGTTGCTCCTGTTCCTTCTGTGATACTTCACATGAACAGTGGGTTCCATGTTAGGGTTTCTAAGAGATGGTCCTGA

mRNA sequence

ATGTCCGGCGACTGGCGGCCACCGCTCGCAGCCGCCGTCGGCCGCCGGAGGCTGACGGCAGCGGCGAGGATGGCCGACGATGGTGGAGACAGGTATATAGTTAACGAATATAACGAGCTCGAATGCACCGACAAGTTCTTGAGAGACACCGTCTTAAACTTCATGATGGCGGGGCGAGACGCTTTCAGCGCAACTCTCTCATGGTTGTTCTTCATCCTCTCAAAGAATCCAATTGTGGTAGAAAAGATCCGAGAAGAGCTCAACGGCACAACAATCCCACAGAGCGAAGAATCTAAAAAACAACCACGAATTTTCACCATTGAAGAGCTCAACGACCTCGTTTACTTGCATGGAGCCTTATGCGAAACGCTTCGTCTCTATCCTCCGATTGCGTTCGAGCACAAATCTCCGGCAGAGGCCGACACCCTTCCGAGCGGCCACCACGTGAAGCCACACACGATGATCCTCTTCTCGTTGTACGCACTGGGAAGAATGAGGTCTGTTTGGGGAGAGGATTGCGAAGAATTCAAACCAGAGAGATGGGTTTCGGAGAAGGGGACGATCAAACGAGAGCCTTCCTATAAGTTCTTCAGTTTCAATGCAGGGCCGAGGAGTTGTTTGGGAAAAGGAGTGGCTTTTTCCCAGTTGAAGATTGTTTCAGCTGCCATTATCCATAACTACGACATTCAAGCTGTTGAAGACAACAACAATGTTGTTGTTGCTCCTGTTCCTTCTGTGATACTTCACATGAACAGTGGGTTCCATGTTAGGGTTTCTAAGAGATGGTCCTGA

Coding sequence (CDS)

ATGTCCGGCGACTGGCGGCCACCGCTCGCAGCCGCCGTCGGCCGCCGGAGGCTGACGGCAGCGGCGAGGATGGCCGACGATGGTGGAGACAGGTATATAGTTAACGAATATAACGAGCTCGAATGCACCGACAAGTTCTTGAGAGACACCGTCTTAAACTTCATGATGGCGGGGCGAGACGCTTTCAGCGCAACTCTCTCATGGTTGTTCTTCATCCTCTCAAAGAATCCAATTGTGGTAGAAAAGATCCGAGAAGAGCTCAACGGCACAACAATCCCACAGAGCGAAGAATCTAAAAAACAACCACGAATTTTCACCATTGAAGAGCTCAACGACCTCGTTTACTTGCATGGAGCCTTATGCGAAACGCTTCGTCTCTATCCTCCGATTGCGTTCGAGCACAAATCTCCGGCAGAGGCCGACACCCTTCCGAGCGGCCACCACGTGAAGCCACACACGATGATCCTCTTCTCGTTGTACGCACTGGGAAGAATGAGGTCTGTTTGGGGAGAGGATTGCGAAGAATTCAAACCAGAGAGATGGGTTTCGGAGAAGGGGACGATCAAACGAGAGCCTTCCTATAAGTTCTTCAGTTTCAATGCAGGGCCGAGGAGTTGTTTGGGAAAAGGAGTGGCTTTTTCCCAGTTGAAGATTGTTTCAGCTGCCATTATCCATAACTACGACATTCAAGCTGTTGAAGACAACAACAATGTTGTTGTTGCTCCTGTTCCTTCTGTGATACTTCACATGAACAGTGGGTTCCATGTTAGGGTTTCTAAGAGATGGTCCTGA

Protein sequence

MSGDWRPPLAAAVGRRRLTAAARMADDGGDRYIVNEYNELECTDKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQPRIFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKRWS
Homology
BLAST of Lag0012950 vs. NCBI nr
Match: TYK12965.1 (alkane hydroxylase MAH1-like [Cucumis melo var. makuwa])

HSP 1 Score: 387.9 bits (995), Expect = 7.0e-104
Identity = 192/258 (74.42%), Postives = 220/258 (85.27%), Query Frame = 0

Query: 12  AVGRRRLTAAARM---ADDGGDR---YIVNEYNELECTDKFLRDTVLNFMMAGRDAFSAT 71
           AV R RL     +    D G D    Y+VNE++E++C DKFLRDTVLNFM+AGRDA S T
Sbjct: 258 AVKRERLQRDREVKEEQDHGADLITWYMVNEHDEIDCNDKFLRDTVLNFMIAGRDALSVT 317

Query: 72  LSWLFFILSKNPIVVEKIREELNG-TTIPQSEESKKQ-PRIFTIEELNDLVYLHGALCET 131
           LSWLFF+LSKNP +V KIREEL G  TI Q+E+ ++Q PR+FTI+ELN+LVYLHGALCET
Sbjct: 318 LSWLFFVLSKNPTIVAKIREELKGIPTISQNEQEQQQKPRVFTIQELNNLVYLHGALCET 377

Query: 132 LRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVS 191
           LRLYPPIAFEHKSP EA+TLPSGHHV+P   ILFSLYALGRMRSVWGEDCEEFKPERW+S
Sbjct: 378 LRLYPPIAFEHKSPVEAETLPSGHHVRPGMRILFSLYALGRMRSVWGEDCEEFKPERWIS 437

Query: 192 EKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPV 251
           +KGTIKREPSYKFFSFNAGPR+CLGKGVAFSQLKIVSAAIIHNYDI+AV++++N  VAP 
Sbjct: 438 DKGTIKREPSYKFFSFNAGPRTCLGKGVAFSQLKIVSAAIIHNYDIEAVDEDSNNAVAPA 497

Query: 252 PSVILHMNSGFHVRVSKR 262
            SVILHM SGF V+V+KR
Sbjct: 498 ASVILHMKSGFRVKVTKR 515

BLAST of Lag0012950 vs. NCBI nr
Match: XP_008440137.2 (PREDICTED: alkane hydroxylase MAH1-like [Cucumis melo])

HSP 1 Score: 387.9 bits (995), Expect = 7.0e-104
Identity = 192/258 (74.42%), Postives = 220/258 (85.27%), Query Frame = 0

Query: 12  AVGRRRLTAAARM---ADDGGDR---YIVNEYNELECTDKFLRDTVLNFMMAGRDAFSAT 71
           AV R RL     +    D G D    Y+VNE++E++C DKFLRDTVLNFM+AGRDA S T
Sbjct: 49  AVKRERLQRDREVKEEQDHGADLITWYMVNEHDEIDCNDKFLRDTVLNFMIAGRDALSVT 108

Query: 72  LSWLFFILSKNPIVVEKIREELNG-TTIPQSEESKKQ-PRIFTIEELNDLVYLHGALCET 131
           LSWLFF+LSKNP +V KIREEL G  TI Q+E+ ++Q PR+FTI+ELN+LVYLHGALCET
Sbjct: 109 LSWLFFVLSKNPTIVAKIREELKGIPTISQNEQEQQQKPRVFTIQELNNLVYLHGALCET 168

Query: 132 LRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVS 191
           LRLYPPIAFEHKSP EA+TLPSGHHV+P   ILFSLYALGRMRSVWGEDCEEFKPERW+S
Sbjct: 169 LRLYPPIAFEHKSPVEAETLPSGHHVRPGMRILFSLYALGRMRSVWGEDCEEFKPERWIS 228

Query: 192 EKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPV 251
           +KGTIKREPSYKFFSFNAGPR+CLGKGVAFSQLKIVSAAIIHNYDI+AV++++N  VAP 
Sbjct: 229 DKGTIKREPSYKFFSFNAGPRTCLGKGVAFSQLKIVSAAIIHNYDIEAVDEDSNNAVAPA 288

Query: 252 PSVILHMNSGFHVRVSKR 262
            SVILHM SGF V+V+KR
Sbjct: 289 ASVILHMKSGFRVKVTKR 306

BLAST of Lag0012950 vs. NCBI nr
Match: XP_023518531.1 (alkane hydroxylase MAH1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387.9 bits (995), Expect = 7.0e-104
Identity = 194/259 (74.90%), Postives = 214/259 (82.63%), Query Frame = 0

Query: 12  AVGRRRLTAAARMAD---DGG----DRYIVNEYNELECTDKFLRDTVLNFMMAGRDAFSA 71
           AV   RL  A  M +   DGG      Y++NE+NEL+  DKFLRDTVLNFM+AGRDAFS 
Sbjct: 258 AVKTERLNRAREMKEELPDGGADLITWYMINEHNELDINDKFLRDTVLNFMIAGRDAFSV 317

Query: 72  TLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQPRIFTIEELNDLVYLHGALCETL 131
           TLSWLF+ L+KNPI++ KIREELN  TIPQ        R F IEEL++LVYLHGA+CETL
Sbjct: 318 TLSWLFYTLTKNPIILAKIREELNSITIPQQ-------RTFAIEELSNLVYLHGAICETL 377

Query: 132 RLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVSE 191
           RLYPPIAFEHKS AEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERW++ 
Sbjct: 378 RLYPPIAFEHKSAAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWITA 437

Query: 192 KGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPVP 251
           KG IKREPSYKFFSFNAGPR+CLGK VAFSQLKIVSAAIIHN+DIQAV+D+   VVAP P
Sbjct: 438 KGAIKREPSYKFFSFNAGPRTCLGKSVAFSQLKIVSAAIIHNFDIQAVDDHK--VVAPAP 497

Query: 252 SVILHMNSGFHVRVSKRWS 264
           SVILHMN GF VRV KRW+
Sbjct: 498 SVILHMNCGFRVRVCKRWA 507

BLAST of Lag0012950 vs. NCBI nr
Match: KAG6595090.1 (Alkane hydroxylase MAH1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 387.9 bits (995), Expect = 7.0e-104
Identity = 194/259 (74.90%), Postives = 215/259 (83.01%), Query Frame = 0

Query: 12  AVGRRRLTAAARMAD---DGG----DRYIVNEYNELECTDKFLRDTVLNFMMAGRDAFSA 71
           AV   RL  A  M +   DGG      Y++NE++EL+  DKFLRDTVLNFM+AGRDAFS 
Sbjct: 258 AVKTERLNRAREMKEELHDGGADLITWYMINEHDELDINDKFLRDTVLNFMIAGRDAFSV 317

Query: 72  TLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQPRIFTIEELNDLVYLHGALCETL 131
           TLSWLF+ L+KNPI++ KIREELN  TIPQ        R F IEEL++LVYLHGA+CETL
Sbjct: 318 TLSWLFYTLTKNPIILAKIREELNSITIPQQ-------RTFAIEELSNLVYLHGAICETL 377

Query: 132 RLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVSE 191
           RLYPPIAFEHKS AEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERW++E
Sbjct: 378 RLYPPIAFEHKSAAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWITE 437

Query: 192 KGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPVP 251
           KG IKREPSYKFFSFNAGPR+CLGK VAFSQLKIVSAAIIHN+DIQAV+D+   VVAP P
Sbjct: 438 KGAIKREPSYKFFSFNAGPRTCLGKSVAFSQLKIVSAAIIHNFDIQAVDDDK--VVAPAP 497

Query: 252 SVILHMNSGFHVRVSKRWS 264
           SVILHMN GF VRV KRW+
Sbjct: 498 SVILHMNCGFKVRVCKRWA 507

BLAST of Lag0012950 vs. NCBI nr
Match: XP_004142038.2 (alkane hydroxylase MAH1 [Cucumis sativus])

HSP 1 Score: 387.5 bits (994), Expect = 9.2e-104
Identity = 191/259 (73.75%), Postives = 217/259 (83.78%), Query Frame = 0

Query: 12  AVGRRRLTAAARMADDGGDR-------YIVNEYNELECTDKFLRDTVLNFMMAGRDAFSA 71
           AV R RL     + +D  D        Y+VNE++E++C DKFLRDTVLNFM+AGRDA S 
Sbjct: 258 AVKRERLQRDREVKEDQQDHGADLITWYMVNEHDEIDCNDKFLRDTVLNFMIAGRDALSV 317

Query: 72  TLSWLFFILSKNPIVVEKIREELNG-TTIPQSEESKKQ-PRIFTIEELNDLVYLHGALCE 131
           TLSW FF+LSKNP +V KIREEL   TTI Q E+ ++Q PR+FTIEELN+LVYLHGALCE
Sbjct: 318 TLSWFFFVLSKNPAIVAKIREELKDITTIQQKEQEQQQKPRVFTIEELNNLVYLHGALCE 377

Query: 132 TLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWV 191
           TLRLYPPIAFEHKSP  A+TLPSGHHV+P T ILFSLYALGRMRSVWGEDCEEFKPERW+
Sbjct: 378 TLRLYPPIAFEHKSPVVAETLPSGHHVRPGTRILFSLYALGRMRSVWGEDCEEFKPERWI 437

Query: 192 SEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAP 251
           S+KGTIKREPSYKFFSFNAGPR+CLGKGVAFSQLKIVSAAI+HNYDI+AV+++ N VV P
Sbjct: 438 SDKGTIKREPSYKFFSFNAGPRTCLGKGVAFSQLKIVSAAIVHNYDIEAVDEDINNVVVP 497

Query: 252 VPSVILHMNSGFHVRVSKR 262
             SVILHM SGF V+VSKR
Sbjct: 498 AASVILHMKSGFRVKVSKR 516

BLAST of Lag0012950 vs. ExPASy Swiss-Prot
Match: Q9FVS9 (Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 3.8e-60
Identity = 108/218 (49.54%), Postives = 150/218 (68.81%), Query Frame = 0

Query: 44  DKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQPR 103
           DKF+RD + + ++AGRD  S+ L+W F++LSK+P V+ K+R E+N               
Sbjct: 296 DKFIRDVIFSLVLAGRDTTSSVLTWFFWLLSKHPQVMAKLRHEINTK------------- 355

Query: 104 IFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALG 163
            F  E+L  LVYLH AL E++RLYPP+ F HKSPA+ D LPSGH V  ++ I+  +YALG
Sbjct: 356 -FDNEDLEKLVYLHAALSESMRLYPPLPFNHKSPAKPDVLPSGHKVDANSKIVICIYALG 415

Query: 164 RMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAI 223
           RMRSVWGED  +FKPERW+S+ G ++ EPSYKF +FN+GPR+CLGK +A  Q+K+V+  I
Sbjct: 416 RMRSVWGEDALDFKPERWISDNGGLRHEPSYKFMAFNSGPRTCLGKNLALLQMKMVALEI 475

Query: 224 IHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKR 262
           I NYD + +E +    V P+PS++L M  G  V V+K+
Sbjct: 476 IRNYDFKVIEGHK---VEPIPSILLRMKHGLKVTVTKK 496

BLAST of Lag0012950 vs. ExPASy Swiss-Prot
Match: A0A140IL91 (Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T2 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 6.9e-54
Identity = 104/220 (47.27%), Postives = 146/220 (66.36%), Query Frame = 0

Query: 44  DKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQP- 103
           DKFLRD    +++A R+  S T++WLF+ L +NP V  KI  EL  + + +S E K    
Sbjct: 295 DKFLRDNAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELK-SIVDESSERKFNDG 354

Query: 104 -RIFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYA 163
             +F    +   +YLH ALCE LR+YPP+ FE K   +AD LPSGH V+    ILFS YA
Sbjct: 355 FTLFDSNMIQSAIYLHAALCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYA 414

Query: 164 LGRMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSA 223
           + RM+ +WG+DC EFKPERW++  GT+K EP+YKFF+F+AGPR CLGK ++F+Q+K+V A
Sbjct: 415 MARMKGIWGDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVA 474

Query: 224 AIIHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKR 262
            II+N+ +Q V+ +   VV    S+++ M  G  V+V KR
Sbjct: 475 TIIYNFHLQMVKGH---VVEQSNSILMDMKHGLMVQVRKR 510

BLAST of Lag0012950 vs. ExPASy Swiss-Prot
Match: A0A140IL90 (Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T1 PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.0e-53
Identity = 103/220 (46.82%), Postives = 145/220 (65.91%), Query Frame = 0

Query: 44  DKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQP- 103
           DKFLRD    +++A R+  S T++WLF+ L +NP V  KI  EL  + + +S E K    
Sbjct: 295 DKFLRDNAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELK-SIVDESSERKFNDG 354

Query: 104 -RIFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYA 163
             +F    +   +YLH  LCE LR+YPP+ FE K   +AD LPSGH V+    ILFS YA
Sbjct: 355 FALFDSNMIQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYA 414

Query: 164 LGRMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSA 223
           + RM+ +WG+DC EFKPERW++  GT+K EP+YKFF+F+AGPR CLGK ++F+Q+K+V A
Sbjct: 415 MARMKGIWGDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVA 474

Query: 224 AIIHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKR 262
            II+N+ +Q V+ +   VV    S+++ M  G  V+V KR
Sbjct: 475 TIIYNFHLQMVKGH---VVEQSNSILMDMKHGLMVQVRKR 510

BLAST of Lag0012950 vs. ExPASy Swiss-Prot
Match: A0A2H5AJ00 (Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.0e-53
Identity = 103/220 (46.82%), Postives = 145/220 (65.91%), Query Frame = 0

Query: 44  DKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQP- 103
           DKFLRD    +++A R+  S T++WLF+ L +NP V  KI  EL  + + +S E K    
Sbjct: 295 DKFLRDNAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELK-SIVDESSERKFNDG 354

Query: 104 -RIFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYA 163
             +F    +   +YLH  LCE LR+YPP+ FE K   +AD LPSGH V+    ILFS YA
Sbjct: 355 FALFDSNMIQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYA 414

Query: 164 LGRMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSA 223
           + RM+ +WG+DC EFKPERW++  GT+K EP+YKFF+F+AGPR CLGK ++F+Q+K+V A
Sbjct: 415 MARMKGIWGDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVA 474

Query: 224 AIIHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKR 262
            II+N+ +Q V+ +   VV    S+++ M  G  V+V KR
Sbjct: 475 TIIYNFHLQMVKGH---VVEQSNSILMDMKHGLMVQVRKR 510

BLAST of Lag0012950 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ9 (Noroxomaritidine synthase 2 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T2 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.0e-53
Identity = 103/220 (46.82%), Postives = 145/220 (65.91%), Query Frame = 0

Query: 44  DKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQP- 103
           DKFLRD    +++A R+  S T++WLF+ L +NP V  KI  EL  + + +S E K    
Sbjct: 295 DKFLRDNAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELK-SIVDESSERKFNDG 354

Query: 104 -RIFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYA 163
             +F    +   +YLH  LCE LR+YPP+ FE K   +AD LPSGH V+    ILFS YA
Sbjct: 355 FALFDSNMIQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYA 414

Query: 164 LGRMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSA 223
           + RM+ +WG+DC EFKPERW++  GT+K EP+YKFF+F+AGPR CLGK ++F+Q+K+V A
Sbjct: 415 MARMKGIWGDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVA 474

Query: 224 AIIHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKR 262
            II+N+ +Q V+ +   VV    S+++ M  G  V+V KR
Sbjct: 475 TIIYNFHLQMVKGH---VVEQSNSILMDMKHGLMVQVRKR 510

BLAST of Lag0012950 vs. ExPASy TrEMBL
Match: A0A5D3CN31 (Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005620 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.4e-104
Identity = 192/258 (74.42%), Postives = 220/258 (85.27%), Query Frame = 0

Query: 12  AVGRRRLTAAARM---ADDGGDR---YIVNEYNELECTDKFLRDTVLNFMMAGRDAFSAT 71
           AV R RL     +    D G D    Y+VNE++E++C DKFLRDTVLNFM+AGRDA S T
Sbjct: 258 AVKRERLQRDREVKEEQDHGADLITWYMVNEHDEIDCNDKFLRDTVLNFMIAGRDALSVT 317

Query: 72  LSWLFFILSKNPIVVEKIREELNG-TTIPQSEESKKQ-PRIFTIEELNDLVYLHGALCET 131
           LSWLFF+LSKNP +V KIREEL G  TI Q+E+ ++Q PR+FTI+ELN+LVYLHGALCET
Sbjct: 318 LSWLFFVLSKNPTIVAKIREELKGIPTISQNEQEQQQKPRVFTIQELNNLVYLHGALCET 377

Query: 132 LRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVS 191
           LRLYPPIAFEHKSP EA+TLPSGHHV+P   ILFSLYALGRMRSVWGEDCEEFKPERW+S
Sbjct: 378 LRLYPPIAFEHKSPVEAETLPSGHHVRPGMRILFSLYALGRMRSVWGEDCEEFKPERWIS 437

Query: 192 EKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPV 251
           +KGTIKREPSYKFFSFNAGPR+CLGKGVAFSQLKIVSAAIIHNYDI+AV++++N  VAP 
Sbjct: 438 DKGTIKREPSYKFFSFNAGPRTCLGKGVAFSQLKIVSAAIIHNYDIEAVDEDSNNAVAPA 497

Query: 252 PSVILHMNSGFHVRVSKR 262
            SVILHM SGF V+V+KR
Sbjct: 498 ASVILHMKSGFRVKVTKR 515

BLAST of Lag0012950 vs. ExPASy TrEMBL
Match: A0A1S3B003 (alkane hydroxylase MAH1-like OS=Cucumis melo OX=3656 GN=LOC103484693 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.4e-104
Identity = 192/258 (74.42%), Postives = 220/258 (85.27%), Query Frame = 0

Query: 12  AVGRRRLTAAARM---ADDGGDR---YIVNEYNELECTDKFLRDTVLNFMMAGRDAFSAT 71
           AV R RL     +    D G D    Y+VNE++E++C DKFLRDTVLNFM+AGRDA S T
Sbjct: 49  AVKRERLQRDREVKEEQDHGADLITWYMVNEHDEIDCNDKFLRDTVLNFMIAGRDALSVT 108

Query: 72  LSWLFFILSKNPIVVEKIREELNG-TTIPQSEESKKQ-PRIFTIEELNDLVYLHGALCET 131
           LSWLFF+LSKNP +V KIREEL G  TI Q+E+ ++Q PR+FTI+ELN+LVYLHGALCET
Sbjct: 109 LSWLFFVLSKNPTIVAKIREELKGIPTISQNEQEQQQKPRVFTIQELNNLVYLHGALCET 168

Query: 132 LRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVS 191
           LRLYPPIAFEHKSP EA+TLPSGHHV+P   ILFSLYALGRMRSVWGEDCEEFKPERW+S
Sbjct: 169 LRLYPPIAFEHKSPVEAETLPSGHHVRPGMRILFSLYALGRMRSVWGEDCEEFKPERWIS 228

Query: 192 EKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPV 251
           +KGTIKREPSYKFFSFNAGPR+CLGKGVAFSQLKIVSAAIIHNYDI+AV++++N  VAP 
Sbjct: 229 DKGTIKREPSYKFFSFNAGPRTCLGKGVAFSQLKIVSAAIIHNYDIEAVDEDSNNAVAPA 288

Query: 252 PSVILHMNSGFHVRVSKR 262
            SVILHM SGF V+V+KR
Sbjct: 289 ASVILHMKSGFRVKVTKR 306

BLAST of Lag0012950 vs. ExPASy TrEMBL
Match: A0A0A0KFE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487020 PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 4.5e-104
Identity = 191/259 (73.75%), Postives = 217/259 (83.78%), Query Frame = 0

Query: 12  AVGRRRLTAAARMADDGGDR-------YIVNEYNELECTDKFLRDTVLNFMMAGRDAFSA 71
           AV R RL     + +D  D        Y+VNE++E++C DKFLRDTVLNFM+AGRDA S 
Sbjct: 258 AVKRERLQRDREVKEDQQDHGADLITWYMVNEHDEIDCNDKFLRDTVLNFMIAGRDALSV 317

Query: 72  TLSWLFFILSKNPIVVEKIREELNG-TTIPQSEESKKQ-PRIFTIEELNDLVYLHGALCE 131
           TLSW FF+LSKNP +V KIREEL   TTI Q E+ ++Q PR+FTIEELN+LVYLHGALCE
Sbjct: 318 TLSWFFFVLSKNPAIVAKIREELKDITTIQQKEQEQQQKPRVFTIEELNNLVYLHGALCE 377

Query: 132 TLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWV 191
           TLRLYPPIAFEHKSP  A+TLPSGHHV+P T ILFSLYALGRMRSVWGEDCEEFKPERW+
Sbjct: 378 TLRLYPPIAFEHKSPVVAETLPSGHHVRPGTRILFSLYALGRMRSVWGEDCEEFKPERWI 437

Query: 192 SEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAP 251
           S+KGTIKREPSYKFFSFNAGPR+CLGKGVAFSQLKIVSAAI+HNYDI+AV+++ N VV P
Sbjct: 438 SDKGTIKREPSYKFFSFNAGPRTCLGKGVAFSQLKIVSAAIVHNYDIEAVDEDINNVVVP 497

Query: 252 VPSVILHMNSGFHVRVSKR 262
             SVILHM SGF V+VSKR
Sbjct: 498 AASVILHMKSGFRVKVSKR 516

BLAST of Lag0012950 vs. ExPASy TrEMBL
Match: A0A6J1HFA2 (alkane hydroxylase MAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111463446 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.8e-103
Identity = 193/259 (74.52%), Postives = 214/259 (82.63%), Query Frame = 0

Query: 12  AVGRRRLTAAARMAD---DGG----DRYIVNEYNELECTDKFLRDTVLNFMMAGRDAFSA 71
           AV   RL  A  M +   DGG      Y++NE++EL+  DKFLRDTVLNFM+AGRDAFS 
Sbjct: 258 AVKTERLNRAREMKEELHDGGADLITWYMINEHDELDINDKFLRDTVLNFMIAGRDAFSV 317

Query: 72  TLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQPRIFTIEELNDLVYLHGALCETL 131
           TLSWLF+ L+KNPI++ KIREELN  TI        Q R F IEEL++LVYLHGA+CETL
Sbjct: 318 TLSWLFYTLTKNPIILAKIREELNSITI-------LQQRTFAIEELSNLVYLHGAICETL 377

Query: 132 RLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVSE 191
           RLYPPIAFEHKS AEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERW++E
Sbjct: 378 RLYPPIAFEHKSAAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWITE 437

Query: 192 KGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPVP 251
           KG IKREPSYKFFSFNAGPR+CLGK VAFSQLKIVSAAIIHN+DIQAV+D+   VVAP P
Sbjct: 438 KGAIKREPSYKFFSFNAGPRTCLGKSVAFSQLKIVSAAIIHNFDIQAVDDDK--VVAPAP 497

Query: 252 SVILHMNSGFHVRVSKRWS 264
           SVILHMN GF VRV KRW+
Sbjct: 498 SVILHMNCGFRVRVCKRWA 507

BLAST of Lag0012950 vs. ExPASy TrEMBL
Match: A0A6J1KPC4 (alkane hydroxylase MAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111497483 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.4e-102
Identity = 186/241 (77.18%), Postives = 207/241 (85.89%), Query Frame = 0

Query: 26  DDGGDR---YIVNEYNELECTDKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEK 85
           D G D    Y++NE++EL+  DKFLRDTVLNFM+AGRDAFS TLSWLF+ILSKNPI++ K
Sbjct: 276 DGGADLITWYMINEHDELDINDKFLRDTVLNFMIAGRDAFSVTLSWLFYILSKNPIILAK 335

Query: 86  IREELNGTTIPQSEESKKQPRIFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADT 145
           IR+ELN  TIPQ        R F  EEL++LVYLHGA+CETLRL+PPIAFEHKS AEADT
Sbjct: 336 IRQELNSITIPQQ-------RTFANEELSNLVYLHGAICETLRLHPPIAFEHKSAAEADT 395

Query: 146 LPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAG 205
           LPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERW++EKG IKREPSYKFFSFNAG
Sbjct: 396 LPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWITEKGAIKREPSYKFFSFNAG 455

Query: 206 PRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKRW 264
           PR+CLGK VAFSQLKIVSAAIIHNYDI+AV+D+   VV P PSVILHMN GF VRV KRW
Sbjct: 456 PRTCLGKSVAFSQLKIVSAAIIHNYDIEAVDDDK--VVTPAPSVILHMNCGFRVRVCKRW 507

BLAST of Lag0012950 vs. TAIR 10
Match: AT2G23180.1 (cytochrome P450, family 96, subfamily A, polypeptide 1 )

HSP 1 Score: 263.1 bits (671), Expect = 2.4e-70
Identity = 123/218 (56.42%), Postives = 159/218 (72.94%), Query Frame = 0

Query: 44  DKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQPR 103
           DKFLRD +L+FM+AGRD  S+ L+W F++LSKNP  + KIR+E+N    P++ +      
Sbjct: 302 DKFLRDMILSFMLAGRDTTSSALTWFFWLLSKNPKAITKIRQEINTQLSPRTNDFDS--- 361

Query: 104 IFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALG 163
            F  +ELN LVY+HGALCE LRLYPP+ F+HKSP ++D LPSGH V   + I+F LY+LG
Sbjct: 362 -FNAQELNKLVYVHGALCEALRLYPPVPFQHKSPTKSDVLPSGHRVDASSKIVFCLYSLG 421

Query: 164 RMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAAI 223
           RM+SVWGED  EFKPERW+SE G +   PS+KF SFNAGPR+CLGK VA +Q+K V+  I
Sbjct: 422 RMKSVWGEDASEFKPERWISESGRLIHVPSFKFLSFNAGPRTCLGKEVAMTQMKTVAVKI 481

Query: 224 IHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKR 262
           I NY+I+ VE +    + PVPS+ILHM  G  V V+KR
Sbjct: 482 IQNYEIKVVEGHK---IEPVPSIILHMKHGLKVTVTKR 512

BLAST of Lag0012950 vs. TAIR 10
Match: AT4G39480.1 (cytochrome P450, family 96, subfamily A, polypeptide 9 )

HSP 1 Score: 256.9 bits (655), Expect = 1.7e-68
Identity = 124/222 (55.86%), Postives = 154/222 (69.37%), Query Frame = 0

Query: 44  DKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQSEES----K 103
           D+FLRDT+L+FM+AGRD   + L+W F++L  N   + KIR+E+N    P+++       
Sbjct: 295 DRFLRDTILSFMLAGRDTTGSALTWFFWLLCNNQEAMTKIRQEINTNLFPRNKTDDGSVS 354

Query: 104 KQPRIFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSL 163
                F  +E+  LVYLHGA+CE LRLYPP+ F HKSPA+ D LPSGH VK ++ ILF L
Sbjct: 355 YDSDSFNPQEVKKLVYLHGAVCEALRLYPPVPFNHKSPAKPDVLPSGHKVKANSRILFCL 414

Query: 164 YALGRMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIV 223
           Y+LGRM+SVWGED  EFKPERW+SE G    EPSYKF SFNAGPR+CLGK VA +Q+K V
Sbjct: 415 YSLGRMKSVWGEDAMEFKPERWISESGRSVHEPSYKFLSFNAGPRTCLGKEVAMTQMKTV 474

Query: 224 SAAIIHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKR 262
           +  II NYDI  VE +    + P PSVILHM  G  V VSKR
Sbjct: 475 AVKIIQNYDINVVEGHK---IKPAPSVILHMKHGLKVTVSKR 513

BLAST of Lag0012950 vs. TAIR 10
Match: AT4G39490.1 (cytochrome P450, family 96, subfamily A, polypeptide 10 )

HSP 1 Score: 255.4 bits (651), Expect = 5.1e-68
Identity = 126/249 (50.60%), Postives = 164/249 (65.86%), Query Frame = 0

Query: 27  DGGDRYIVNEYNELECT---------DKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNP 86
           D   + ++  Y  L+ T         ++FLRDT+L FM+AGRD   + L+W F++L KNP
Sbjct: 271 DSHSKDLLTSYMNLDTTKYKLLNPSDERFLRDTILTFMLAGRDTTGSGLTWFFWLLIKNP 330

Query: 87  IVVEKIREELNGTTIPQSE-----ESKKQPRIFTIEELNDLVYLHGALCETLRLYPPIAF 146
            V+ KIR+E+N     +S+      +      F+ +EL  LVYLHGA+CE+LRLYPP+ F
Sbjct: 331 EVIAKIRQEINTALFQRSKVDDDASNNNDSDSFSPQELKKLVYLHGAICESLRLYPPVPF 390

Query: 147 EHKSPAEADTLPSGHHVKPHTMILFSLYALGRMRSVWGEDCEEFKPERWVSEKGTIKREP 206
           +HKSP + D LPSGH V  ++ ILF LY+LGRM+SVWGED  EFKPERW+SE G    EP
Sbjct: 391 QHKSPTKPDVLPSGHKVDANSKILFCLYSLGRMKSVWGEDALEFKPERWISESGNSVHEP 450

Query: 207 SYKFFSFNAGPRSCLGKGVAFSQLKIVSAAIIHNYDIQAVEDNNNVVVAPVPSVILHMNS 262
           SYKF SFNAGPR+CLGK VA  Q+K V+  II NY+++ VE      + P PSVILHM  
Sbjct: 451 SYKFLSFNAGPRTCLGKEVAMMQMKSVAVKIIQNYEMKIVEGQQ---IEPAPSVILHMKH 510

BLAST of Lag0012950 vs. TAIR 10
Match: AT2G21910.1 (cytochrome P450, family 96, subfamily A, polypeptide 5 )

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-66
Identity = 124/219 (56.62%), Postives = 158/219 (72.15%), Query Frame = 0

Query: 44  DKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQSEESKKQPR 103
           D FL+D + +FM+AGRDA + TL+W F++LSKNP  V KIR+E+N T +P S  S     
Sbjct: 298 DNFLKDIIKSFMLAGRDAIATTLTWFFWLLSKNPEAVTKIRQEIN-TNLPGSGMS----- 357

Query: 104 IFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTMILFSLYALG 163
               ++LN +VYLHGALCE+LRLY PI FE K+P + D LPSGH V  +  ILFS+YALG
Sbjct: 358 -LDADKLNKMVYLHGALCESLRLYAPIPFERKTPIKQDVLPSGHMVDKNWKILFSVYALG 417

Query: 164 RMRSVWGEDCEEFKPERWVSEK-GTIKREPSYKFFSFNAGPRSCLGKGVAFSQLKIVSAA 223
           RMRSVWG+D  EFKPERW+SE+ G +K EPS+KFF FN+GPR+CLGK ++F Q+K V+  
Sbjct: 418 RMRSVWGQDASEFKPERWISERNGGLKHEPSFKFFVFNSGPRNCLGKNLSFLQMKTVAVE 477

Query: 224 IIHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSKR 262
           II NYDI+ VE +    + P  S+ILHM  G  V VSKR
Sbjct: 478 IIRNYDIKVVEGHK---IEPASSIILHMKHGLKVTVSKR 506

BLAST of Lag0012950 vs. TAIR 10
Match: AT1G65340.1 (cytochrome P450, family 96, subfamily A, polypeptide 3 )

HSP 1 Score: 249.6 bits (636), Expect = 2.8e-66
Identity = 119/226 (52.65%), Postives = 158/226 (69.91%), Query Frame = 0

Query: 36  EYNELE-CTDKFLRDTVLNFMMAGRDAFSATLSWLFFILSKNPIVVEKIREELNGTTIPQ 95
           +Y  LE   D+F++DT+L F++A RD  S+ L+W F+++SKNP  + KIR+E+N      
Sbjct: 291 KYKYLEPSDDRFIKDTILGFLIAARDTTSSALTWFFWLMSKNPEAINKIRQEVN------ 350

Query: 96  SEESKKQPRIFTIEELNDLVYLHGALCETLRLYPPIAFEHKSPAEADTLPSGHHVKPHTM 155
               KK PR F   +L  LVYLHGA+CETLRLYPP+ F HKSPA+ D LPSGH V     
Sbjct: 351 ----KKMPR-FDPADLEKLVYLHGAVCETLRLYPPVPFNHKSPAKPDVLPSGHRVDEKWK 410

Query: 156 ILFSLYALGRMRSVWGEDCEEFKPERWVSEKGTIKREPSYKFFSFNAGPRSCLGKGVAFS 215
           I+ S+YALGRM+SVWG+D E+F+PERW+S+ G +K EPSYKF +FNAGPR+CLGK + F 
Sbjct: 411 IVISMYALGRMKSVWGDDAEDFRPERWISDSGRLKHEPSYKFLAFNAGPRACLGKKLTFL 470

Query: 216 QLKIVSAAIIHNYDIQAVEDNNNVVVAPVPSVILHMNSGFHVRVSK 261
           Q+K V+A II NYDI+ VE +      PVPSV+  M  G  V +++
Sbjct: 471 QMKTVAAEIIRNYDIKVVEGHK---TEPVPSVLFRMQHGLKVNITR 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK12965.17.0e-10474.42alkane hydroxylase MAH1-like [Cucumis melo var. makuwa][more]
XP_008440137.27.0e-10474.42PREDICTED: alkane hydroxylase MAH1-like [Cucumis melo][more]
XP_023518531.17.0e-10474.90alkane hydroxylase MAH1-like [Cucurbita pepo subsp. pepo][more]
KAG6595090.17.0e-10474.90Alkane hydroxylase MAH1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_004142038.29.2e-10473.75alkane hydroxylase MAH1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9FVS93.8e-6049.54Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1[more]
A0A140IL916.9e-5447.27Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222... [more]
A0A140IL902.0e-5346.82Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 G... [more]
A0A2H5AJ002.0e-5346.82Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=... [more]
A0A2H5AIZ92.0e-5346.82Noroxomaritidine synthase 2 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T2 PE=... [more]
Match NameE-valueIdentityDescription
A0A5D3CN313.4e-10474.42Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3B0033.4e-10474.42alkane hydroxylase MAH1-like OS=Cucumis melo OX=3656 GN=LOC103484693 PE=3 SV=1[more]
A0A0A0KFE24.5e-10473.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487020 PE=3 SV=1[more]
A0A6J1HFA23.8e-10374.52alkane hydroxylase MAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111463446 PE=3 ... [more]
A0A6J1KPC41.4e-10277.18alkane hydroxylase MAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111497483 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT2G23180.12.4e-7056.42cytochrome P450, family 96, subfamily A, polypeptide 1 [more]
AT4G39480.11.7e-6855.86cytochrome P450, family 96, subfamily A, polypeptide 9 [more]
AT4G39490.15.1e-6850.60cytochrome P450, family 96, subfamily A, polypeptide 10 [more]
AT2G21910.11.6e-6656.62cytochrome P450, family 96, subfamily A, polypeptide 5 [more]
AT1G65340.12.8e-6652.65cytochrome P450, family 96, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 197..206
score: 48.28
coord: 206..217
score: 36.7
coord: 57..74
score: 33.72
coord: 118..129
score: 38.96
IPR001128Cytochrome P450PFAMPF00067p450coord: 36..236
e-value: 1.8E-43
score: 149.0
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 66..92
score: 29.09
coord: 196..206
score: 45.64
coord: 206..229
score: 24.72
coord: 46..63
score: 26.24
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 5..262
e-value: 1.3E-58
score: 201.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 26..261
NoneNo IPR availablePANTHERPTHR24296:SF1CYTOCHROME P450, FAMILY 96, SUBFAMILY A, POLYPEPTIDE 10coord: 18..259
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 18..259
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 199..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012950.1Lag0012950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen