Lag0012792 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012792
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr1: 44396115 .. 44397070 (-)
RNA-Seq ExpressionLag0012792
SyntenyLag0012792
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAAATCCTCATCTACCAAAAGGTGAATTTCCTCTCCGTTTCCTTCATCTCTTCGATCCTTTCAATTCCCTTCCACAATCCTCGGAAACAGAGACGAAAAAATCGAACCTCTGTTCGAAAATCCTTCACCTACATTGCTTCATTTTGCAGGTTGGAAGGCAAAGTCGCCGTAATCACCGGCGCCGCCAGTGGATTCGGCAAGAGCACCGCCAGATTGTTCGTTAAACACGGCGCCAAGGTGATCGTCGCCGACGTGCAGGACGATCTCGGCCGAGCACTCTGCGAAGAACTCGGATCGGAGAAAACCGCTTCGTATGTACACTGCGACGTGACGATCGATTCAGACGTGAAGAACGCCGTCGATTTCGCCGTCGAGAGGTTCGGGAAGCTAGACATAATGTACAACAATGCCGGAATCACCGGCGAGATGGATCCGACGATCTTAGGAACAGATGGGGAGAATTTCAAGAGGGTTTTCGAGGTGAATGTGTACGGCGGGTTTTTAGGAGCGAAACATGCGGCTAGGGTTATGATTCCGAGGAGGAGCGGCGTGATTCTGTTCACATCGAGTGTTGCTTCGGTGAATAGCGGCGAATCGCCGCATGCTTATGCAATGTCGAAGCACGCGGTGGTGGGATTGATGAGGAATTTGTGTGTGGAATTGGGGGAATTTGGAATTAGGGTTAATTCGGTTTCTCCGGGGGCTATTGGAACGCCGTTGCTGAGAAATGCGTTGGGTTTTACGGAGGAGATGCTGGAGGAGGTGGTCCGGTCGTCGGCGATCTTGAAAGGGGCGGTGCCGACGGCGGAGGATGTGGCGGAGGCGGCGCTCTACTTGTGTAGTGATGAATCCAGGGTCATTAGTGGTCATAACCTGGTGGTCGACGGCGGCTACAGCACCGCCAACCGGGCTTTCTCCGCCGCCGCAATCAGAAAAATGTCCTCTGTTTAA

mRNA sequence

ATGGACAAATCCTCATCTACCAAAAGGTTGGAAGGCAAAGTCGCCGTAATCACCGGCGCCGCCAGTGGATTCGGCAAGAGCACCGCCAGATTGTTCGTTAAACACGGCGCCAAGGTGATCGTCGCCGACGTGCAGGACGATCTCGGCCGAGCACTCTGCGAAGAACTCGGATCGGAGAAAACCGCTTCGTATGTACACTGCGACGTGACGATCGATTCAGACGTGAAGAACGCCGTCGATTTCGCCGTCGAGAGGTTCGGGAAGCTAGACATAATGTACAACAATGCCGGAATCACCGGCGAGATGGATCCGACGATCTTAGGAACAGATGGGGAGAATTTCAAGAGGGTTTTCGAGGTGAATGTGTACGGCGGGTTTTTAGGAGCGAAACATGCGGCTAGGGTTATGATTCCGAGGAGGAGCGGCGTGATTCTGTTCACATCGAGTGTTGCTTCGGTGAATAGCGGCGAATCGCCGCATGCTTATGCAATGTCGAAGCACGCGGTGGTGGGATTGATGAGGAATTTGTGTGTGGAATTGGGGGAATTTGGAATTAGGGTTAATTCGGTTTCTCCGGGGGCTATTGGAACGCCGTTGCTGAGAAATGCGTTGGGTTTTACGGAGGAGATGCTGGAGGAGGTGGTCCGGTCGTCGGCGATCTTGAAAGGGGCGGTGCCGACGGCGGAGGATGTGGCGGAGGCGGCGCTCTACTTGTGTAGTGATGAATCCAGGGTCATTAGTGGTCATAACCTGGTGGTCGACGGCGGCTACAGCACCGCCAACCGGGCTTTCTCCGCCGCCGCAATCAGAAAAATGTCCTCTGTTTAA

Coding sequence (CDS)

ATGGACAAATCCTCATCTACCAAAAGGTTGGAAGGCAAAGTCGCCGTAATCACCGGCGCCGCCAGTGGATTCGGCAAGAGCACCGCCAGATTGTTCGTTAAACACGGCGCCAAGGTGATCGTCGCCGACGTGCAGGACGATCTCGGCCGAGCACTCTGCGAAGAACTCGGATCGGAGAAAACCGCTTCGTATGTACACTGCGACGTGACGATCGATTCAGACGTGAAGAACGCCGTCGATTTCGCCGTCGAGAGGTTCGGGAAGCTAGACATAATGTACAACAATGCCGGAATCACCGGCGAGATGGATCCGACGATCTTAGGAACAGATGGGGAGAATTTCAAGAGGGTTTTCGAGGTGAATGTGTACGGCGGGTTTTTAGGAGCGAAACATGCGGCTAGGGTTATGATTCCGAGGAGGAGCGGCGTGATTCTGTTCACATCGAGTGTTGCTTCGGTGAATAGCGGCGAATCGCCGCATGCTTATGCAATGTCGAAGCACGCGGTGGTGGGATTGATGAGGAATTTGTGTGTGGAATTGGGGGAATTTGGAATTAGGGTTAATTCGGTTTCTCCGGGGGCTATTGGAACGCCGTTGCTGAGAAATGCGTTGGGTTTTACGGAGGAGATGCTGGAGGAGGTGGTCCGGTCGTCGGCGATCTTGAAAGGGGCGGTGCCGACGGCGGAGGATGTGGCGGAGGCGGCGCTCTACTTGTGTAGTGATGAATCCAGGGTCATTAGTGGTCATAACCTGGTGGTCGACGGCGGCTACAGCACCGCCAACCGGGCTTTCTCCGCCGCCGCAATCAGAAAAATGTCCTCTGTTTAA

Protein sequence

MDKSSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEKTASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEVNVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCSDESRVISGHNLVVDGGYSTANRAFSAAAIRKMSSV
Homology
BLAST of Lag0012792 vs. NCBI nr
Match: KAG6604329.1 (hypothetical protein SDJN03_04938, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 500.0 bits (1286), Expect = 1.3e-137
Identity = 252/274 (91.97%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MDKSSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEK 60
           MD+S S+KRL GKVA+ITGAASGFGKSTARLFVKHGA V+VADV DDLGR+LC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEK 60

Query: 61  TASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEV 120
           T SYVHCDVT DSDVKNAVDFAVER+GKLDIMYNNAGITGEMDPTILGTDGENFK+VFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAI TPLLRNALGFTEE LEEVVRSSAILKG VPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           DESRVISGHNLVVDGGYSTANR+FSAA IRK+SS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

BLAST of Lag0012792 vs. NCBI nr
Match: XP_022979096.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 498.4 bits (1282), Expect = 3.9e-137
Identity = 251/274 (91.61%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MDKSSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEK 60
           MD+S S+KRL GKVA+ITGAASGFGKSTARLFVKHGA V+VADV D+LGR+LC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDELGRSLCQELGSEK 60

Query: 61  TASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEV 120
           T SYVHCDVT DSDVKNAVDFAVER+GKLDIMYNNAGITGEMDPTILGTDGENFK+VFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAI TPLLRNALGFTEE LEEVVRSSAILKG VPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           DESRVISGHNLVVDGGYSTANR+FSAA IRK+SS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

BLAST of Lag0012792 vs. NCBI nr
Match: XP_023543891.1 (secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 497.7 bits (1280), Expect = 6.6e-137
Identity = 250/274 (91.24%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MDKSSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEK 60
           MD+S S+KRL GKVA+ITGAASGFGKSTARLFVKHGA V+VADV DDLGR+LC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEK 60

Query: 61  TASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEV 120
           T SYVHCDVT DSDVKN+VDFAVER+GKLDIMYNNAGITGEMDPTILGTDGENFK+VFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNSVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAI TPLLRNALGFTEE LEE+VRSSAILKG VPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEELVRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           DESRVISGHNLVVDGGYSTANR+FSAA IRK+SS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

BLAST of Lag0012792 vs. NCBI nr
Match: XP_022925996.1 (secoisolariciresinol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 497.3 bits (1279), Expect = 8.6e-137
Identity = 249/274 (90.88%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MDKSSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEK 60
           MD+S S+KRL GKVA+ITGAASGFGKSTARLFVKHGA V+VADV DDLGR+LC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEK 60

Query: 61  TASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEV 120
           T SY+HCDVT DSDVKNAVDFAVER+GKLDIMYNNAGITGEMDPTILGTDGENFK+VFEV
Sbjct: 61  TVSYLHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIPRRSG+ILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGLILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAI TPLLRNALGFTEE LEEV+RSSAILKG VPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVIRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           DESRVISGHNLVVDGGYSTANR+FSAA IRK+SS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

BLAST of Lag0012792 vs. NCBI nr
Match: XP_038882752.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-134
Identity = 247/274 (90.15%), Postives = 260/274 (94.89%), Query Frame = 0

Query: 1   MDKSSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEK 60
           MD+S S+KRL GKVA+ITG ASGFGKSTARLFV+HGA V++ADVQDDLGR+LCEELGSEK
Sbjct: 1   MDESYSSKRLNGKVAIITGGASGFGKSTARLFVRHGATVVIADVQDDLGRSLCEELGSEK 60

Query: 61  TASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEV 120
           T SYVHCDVT DSDVKNAVDFAVER+G+LDIMYNNAGITGEMDPTILGT  ENFK+VFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNAVDFAVERYGQLDIMYNNAGITGEMDPTILGTKKENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIP RSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCS 240
           GEFGIRVNSVSPGAI TPLLRNALGFTEE LEEVVRSSAILKG VPT EDVAEAAL+LCS
Sbjct: 181 GEFGIRVNSVSPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTVEDVAEAALFLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           DESRVISGHNLVVDGGYSTANR+FS AAIRK+SS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSVAAIRKLSS 274

BLAST of Lag0012792 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.0e-75
Identity = 139/264 (52.65%), Postives = 184/264 (69.70%), Query Frame = 0

Query: 5   SSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEKTASY 64
           SST RL+ KVA+ITG A G G++TA+LFV++GAKV++AD+ DD G+ +C  +GS    S+
Sbjct: 9   SSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISF 68

Query: 65  VHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEVNVYG 124
           VHCDVT D DV+N VD  + + GKLDIM+ N G+      +IL    E+FKRV ++NVYG
Sbjct: 69  VHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYG 128

Query: 125 GFLGAKHAARVMIPRRSGVILFTSSVASVNSGES-PHAYAMSKHAVVGLMRNLCVELGEF 184
            FL AKHAARVMIP + G I+FT+S++S  +GE   H Y  +KHAV+GL  +LC ELGE+
Sbjct: 129 AFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEY 188

Query: 185 GIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCSDES 244
           GIRVN VSP  + +PLL +  G     +EE+   +A LKG +  AEDVA+A  YL  DES
Sbjct: 189 GIRVNCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKGTLLRAEDVADAVAYLAGDES 248

Query: 245 RVISGHNLVVDGGYSTANRAFSAA 268
           + +SG NLV+DGGY+  N AF  A
Sbjct: 249 KYVSGLNLVIDGGYTRTNPAFPTA 272

BLAST of Lag0012792 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 7.5e-75
Identity = 138/265 (52.08%), Postives = 184/265 (69.43%), Query Frame = 0

Query: 4   SSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEKTAS 63
           +SST RL+ KVA+ITG A G G++TA+LFV++GAKV++AD+ DD G+ +C  +GS    S
Sbjct: 8   ASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVIS 67

Query: 64  YVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEVNVY 123
           +VHCDVT D DV+N VD  + + GKLDIM+ N G+      +IL    E+FKRV ++NVY
Sbjct: 68  FVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVY 127

Query: 124 GGFLGAKHAARVMIPRRSGVILFTSSVASVNSGES-PHAYAMSKHAVVGLMRNLCVELGE 183
           G FL AKHAARVMIP + G I+FT+S++S  +GE   H Y  +KHAV+GL  +LC ELG+
Sbjct: 128 GAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQ 187

Query: 184 FGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCSDE 243
            GIRVN VSP  + +PLL +  G     +EE+   +A LKG +  AEDVA+A  YL  DE
Sbjct: 188 HGIRVNCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKGILLRAEDVADAVAYLAGDE 247

Query: 244 SRVISGHNLVVDGGYSTANRAFSAA 268
           S+ +SG NLV+DGGY+  N AF  A
Sbjct: 248 SKYVSGLNLVIDGGYTRTNPAFPTA 272

BLAST of Lag0012792 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.7e-72
Identity = 145/262 (55.34%), Postives = 183/262 (69.85%), Query Frame = 0

Query: 4   SSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEKTAS 63
           S+  ++L GKVAVITG ASG G  TARLFVKHGA+V+VAD+QD+LG +L  ELG +  +S
Sbjct: 9   SADARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPD-ASS 68

Query: 64  YVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEVNVY 123
           YVHCDVT + DV  AVD AV RFGKLD+M+NNAG++G     +     E+F+RV  VN+ 
Sbjct: 69  YVHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLV 128

Query: 124 GGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEF 183
           G FLG KHAARVM P R G I+ T+S++S  SG + HAY  SKHA+VG   N   ELG  
Sbjct: 129 GPFLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRH 188

Query: 184 GIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGA-VPTAEDVAEAALYLCSDE 243
           GIRVN VSP  + TPL R A+G  +E +E ++ +SA LKGA    A+D+A AAL+L SD+
Sbjct: 189 GIRVNCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDD 248

Query: 244 SRVISGHNLVVDGGYSTANRAF 265
            R +SG NL VDGG S  N +F
Sbjct: 249 GRYVSGQNLRVDGGLSVVNSSF 269

BLAST of Lag0012792 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.3e-71
Identity = 142/255 (55.69%), Postives = 182/255 (71.37%), Query Frame = 0

Query: 8   KRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEKTASYVHC 67
           +RLEGKVA+ITG ASG G++TA+LF +HGAKV +ADVQD+LG ++ E +G+   ++Y+HC
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGT-SNSTYIHC 72

Query: 68  DVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEVNVYGGFL 127
           DVT +  VKNAVD  V  +GKLDIM++NAGI+    P I+  +  +F+RVF VNV G FL
Sbjct: 73  DVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFL 132

Query: 128 GAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFGIRV 187
             KHAARVMIP RSG I+ T+S++S   G S HAY  SKHAV+GL RNL VELG+FGIRV
Sbjct: 133 CMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRV 192

Query: 188 NSVSPGAIGTPLLRNALGF-TEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCSDESRVI 247
           N +SP  + T L +   G   EE  E V+  +  LKG     EDVA AALYL SDE++ +
Sbjct: 193 NCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASDEAKYV 252

Query: 248 SGHNLVVDGGYSTAN 262
           SGHNL +DGG+S  N
Sbjct: 253 SGHNLFIDGGFSVCN 266

BLAST of Lag0012792 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.6e-69
Identity = 138/260 (53.08%), Postives = 179/260 (68.85%), Query Frame = 0

Query: 9   RLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEKTASYVHCD 68
           RLEGKVA++TG ASG G+S ARLF++HGAK+ + DVQD+LG+ + + LG +  A Y HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 69  VTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEVNVYGGFLG 128
           VT++ DV+ AVDF  E++G +DIM NNAGITG+    I   D   FK+VF++NV G FLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 129 AKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFGIRVN 188
            KHAAR+MIP+  G I+  +SV+SV +G  PH Y  +KHAVVGL +++  ELG  GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 189 SVSPGAIGTPLLRNALGFTEEMLEEV-------VRSSAILKGAVPTAEDVAEAALYLCSD 248
            VSP A+ T L    L    EM E+        VRS+A LKG      DVAEA LYL ++
Sbjct: 182 CVSPYAVPTRLSMPYLP-ESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLATE 241

Query: 249 ESRVISGHNLVVDGGYSTAN 262
           ES+ +SG NLV+DGG+S AN
Sbjct: 242 ESKYVSGLNLVIDGGFSIAN 260

BLAST of Lag0012792 vs. ExPASy TrEMBL
Match: A0A6J1IPT5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111478837 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.9e-137
Identity = 251/274 (91.61%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MDKSSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEK 60
           MD+S S+KRL GKVA+ITGAASGFGKSTARLFVKHGA V+VADV D+LGR+LC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDELGRSLCQELGSEK 60

Query: 61  TASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEV 120
           T SYVHCDVT DSDVKNAVDFAVER+GKLDIMYNNAGITGEMDPTILGTDGENFK+VFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAI TPLLRNALGFTEE LEEVVRSSAILKG VPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           DESRVISGHNLVVDGGYSTANR+FSAA IRK+SS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

BLAST of Lag0012792 vs. ExPASy TrEMBL
Match: A0A6J1EDN5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111433246 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.2e-137
Identity = 249/274 (90.88%), Postives = 263/274 (95.99%), Query Frame = 0

Query: 1   MDKSSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEK 60
           MD+S S+KRL GKVA+ITGAASGFGKSTARLFVKHGA V+VADV DDLGR+LC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEK 60

Query: 61  TASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEV 120
           T SY+HCDVT DSDVKNAVDFAVER+GKLDIMYNNAGITGEMDPTILGTDGENFK+VFEV
Sbjct: 61  TVSYLHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIPRRSG+ILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGLILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAI TPLLRNALGFTEE LEEV+RSSAILKG VPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVIRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           DESRVISGHNLVVDGGYSTANR+FSAA IRK+SS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

BLAST of Lag0012792 vs. ExPASy TrEMBL
Match: A0A6J1BXW7 (momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111005735 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.6e-133
Identity = 244/274 (89.05%), Postives = 262/274 (95.62%), Query Frame = 0

Query: 1   MDKSSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEK 60
           MD+SSS KRLEGKVA+ITG ASGFG+STARLFV+HGAKVIVADVQD+LGR+LC+ELG EK
Sbjct: 33  MDESSSAKRLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEK 92

Query: 61  TASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEV 120
           T SYVHCDVT DSDVKNAVD AV ++GKLDIMYNNAGITGEMDPTILGT+GENFK+VFEV
Sbjct: 93  TVSYVHCDVTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEV 152

Query: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIP+R GVILFT+SVASVNSGESPHAYAMSK+AVVGLMRNLCVEL
Sbjct: 153 NVYGGFLGAKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVEL 212

Query: 181 GEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCS 240
           GEFGIRVNSVSPGAI TPLLRNALGFTEE LEEV+RS+AILKGAVPTAEDVAEAALYLCS
Sbjct: 213 GEFGIRVNSVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCS 272

Query: 241 DESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           DESRVISGHNLVVDGGYSTAN +FSAA IRK+SS
Sbjct: 273 DESRVISGHNLVVDGGYSTANLSFSAAVIRKLSS 306

BLAST of Lag0012792 vs. ExPASy TrEMBL
Match: A0A5A7SIU8 (Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001660 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.8e-131
Identity = 242/275 (88.00%), Postives = 262/275 (95.27%), Query Frame = 0

Query: 1   MDKSS-STKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSE 60
           MD+S  S+KRL GKVA+ITGAASGFGKSTARLFV+HGA+V++ADVQD+L + LC+ELGSE
Sbjct: 1   MDQSMISSKRLNGKVALITGAASGFGKSTARLFVQHGARVVLADVQDNLAQPLCKELGSE 60

Query: 61  KTASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFE 120
           ++ SY+HCDVT DSDVKNAVDFAVER+GKLDIMYNNAGITG+MDPTILGTDGENFK+VFE
Sbjct: 61  ESVSYIHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGQMDPTILGTDGENFKKVFE 120

Query: 121 VNVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180
           VNVYGGFLGAKHAARVMIP RSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE
Sbjct: 121 VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180

Query: 181 LGEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLC 240
           LGEFGIRVNSVSPGAI TPLLRNALGFT++ LEEVVRSSAILKG V TAEDVAEAALYLC
Sbjct: 181 LGEFGIRVNSVSPGAIATPLLRNALGFTKKELEEVVRSSAILKGVVATAEDVAEAALYLC 240

Query: 241 SDESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           SDESRVISGHNLVVDGGYSTANR+FSAA IRK+SS
Sbjct: 241 SDESRVISGHNLVVDGGYSTANRSFSAAVIRKVSS 275

BLAST of Lag0012792 vs. ExPASy TrEMBL
Match: A0A1S3CHD5 (secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103500923 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.8e-131
Identity = 242/275 (88.00%), Postives = 262/275 (95.27%), Query Frame = 0

Query: 1   MDKSS-STKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSE 60
           MD+S  S+KRL GKVA+ITGAASGFGKSTARLFV+HGA+V++ADVQD+L + LC+ELGSE
Sbjct: 1   MDQSMISSKRLNGKVALITGAASGFGKSTARLFVQHGARVVLADVQDNLAQPLCKELGSE 60

Query: 61  KTASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFE 120
           ++ SY+HCDVT DSDVKNAVDFAVER+GKLDIMYNNAGITG+MDPTILGTDGENFK+VFE
Sbjct: 61  ESVSYIHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGQMDPTILGTDGENFKKVFE 120

Query: 121 VNVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180
           VNVYGGFLGAKHAARVMIP RSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE
Sbjct: 121 VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180

Query: 181 LGEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLC 240
           LGEFGIRVNSVSPGAI TPLLRNALGFT++ LEEVVRSSAILKG V TAEDVAEAALYLC
Sbjct: 181 LGEFGIRVNSVSPGAIATPLLRNALGFTKKELEEVVRSSAILKGVVATAEDVAEAALYLC 240

Query: 241 SDESRVISGHNLVVDGGYSTANRAFSAAAIRKMSS 275
           SDESRVISGHNLVVDGGYSTANR+FSAA IRK+SS
Sbjct: 241 SDESRVISGHNLVVDGGYSTANRSFSAAVIRKVSS 275

BLAST of Lag0012792 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 7.7e-67
Identity = 130/257 (50.58%), Postives = 176/257 (68.48%), Query Frame = 0

Query: 6   STKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEKTASYV 65
           ++K+LEGKVA+ITG ASG GK+TA  F++HGA+V++AD+  + G    +ELGSE  A +V
Sbjct: 37  TSKKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELGSE--AEFV 96

Query: 66  HCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDP-TILGTDGENFKRVFEVNVYG 125
            CDVT+++D+  AV+  VER+GKLD+MYNNAGI G M P +I   D   F+RV  +NV+G
Sbjct: 97  RCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFG 156

Query: 126 GFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFG 185
              G KHAA+ MIP RSG IL TSSVA V  G +PH+Y +SK    G++++   EL E G
Sbjct: 157 VVSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHG 216

Query: 186 IRVNSVSPGAIGTPLLRNAL-----GFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLC 245
           +R+N +SPG + TPL  + L       +EE L E V+    LKGA     DVA+AALYL 
Sbjct: 217 VRINCISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELKGAECEEADVAKAALYLA 276

Query: 246 SDESRVISGHNLVVDGG 257
           S++ + ++GHNLVVDGG
Sbjct: 277 SNDGKYVTGHNLVVDGG 291

BLAST of Lag0012792 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.0e-66
Identity = 138/278 (49.64%), Postives = 184/278 (66.19%), Query Frame = 0

Query: 1   MDKSSST---KRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELG 60
           M+++++T   KRLEGKVA+ITG A G GK+T  LF +HGA V++ADV +  G +L + L 
Sbjct: 20  MEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLS 79

Query: 61  SEKTA---SYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEM--DPTILGTDGE 120
           S KT+   +++ CDV++++DV+N V+  V R+G+LDI++NNAG+ G+     +IL  D +
Sbjct: 80  SHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDAD 139

Query: 121 NFKRVFEVNVYGGFLGAKHAARVMIPRR-SGVILFTSSVASVNSGESPHAYAMSKHAVVG 180
            F  V  VNV G  LG KH AR MI R   G I+ T+SVA V  G  PHAY  SKHA+VG
Sbjct: 140 EFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVG 199

Query: 181 LMRNLCVELGEFGIRVNSVSPGAIGTPLLRNALGFTE---------EMLEEVVRSSAILK 240
           L +N   ELG++GIRVN +SP  + T +L NA   T          E +EE VRS A LK
Sbjct: 200 LTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLK 259

Query: 241 GAVPTAEDVAEAALYLCSDESRVISGHNLVVDGGYSTA 261
           G    A D+AEAALYL SDES+ ++GHNLVVDGG +TA
Sbjct: 260 GETLRANDIAEAALYLASDESKYVNGHNLVVDGGVTTA 297

BLAST of Lag0012792 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 243.0 bits (619), Expect = 2.7e-64
Identity = 134/271 (49.45%), Postives = 174/271 (64.21%), Query Frame = 0

Query: 4   SSSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEEL---GSEK 63
           S  ++RL GKVA+ITG A+G G+S  RLF KHGAKV + D+QDDLG  +C+ L    S++
Sbjct: 12  SLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKE 71

Query: 64  TASYVHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEV 123
           TA ++H DV ++ D+ NAVDFAV+ FG LDI+ NNAG+ G   P I       F+  F+V
Sbjct: 72  TAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDV 131

Query: 124 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 183
           NV G FL  KHAARVMIP + G I+   SV  V  G  PH+Y  SKHAV+GL R++  EL
Sbjct: 132 NVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAEL 191

Query: 184 GEFGIRVNSVSPGAIGTPLLRNALGFTEEMLEEV-------VRSSAILKGAVPTAEDVAE 243
           G+ GIRVN VSP A+ T L    L   EE  E+          ++A LKG   T +DVA 
Sbjct: 192 GQHGIRVNCVSPYAVATKLALAHLP-EEERTEDAFVGFRNFAAANANLKGVELTVDDVAN 251

Query: 244 AALYLCSDESRVISGHNLVVDGGYSTANRAF 265
           A L+L SD+SR ISG NL++DGG++  N +F
Sbjct: 252 AVLFLASDDSRYISGDNLMIDGGFTCTNHSF 281

BLAST of Lag0012792 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 240.0 bits (611), Expect = 2.3e-63
Identity = 128/260 (49.23%), Postives = 174/260 (66.92%), Query Frame = 0

Query: 5   SSTKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEKTASY 64
           S++++LEGKVA+ITG ASG GK+TA  F+ HGAKVI+AD+Q  +GR   +ELG   + +Y
Sbjct: 73  STSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELG--PSCAY 132

Query: 65  VHCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEVNVYG 124
             CDVT +SD+ NAVDFAV    KLDIMYNNAGI  +  P+I+  D   F +V   NV G
Sbjct: 133 FPCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVINTNVRG 192

Query: 125 GFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFG 184
              G KHAARVMIPR SG I+   SV  +  G + H Y++SK AV+G++R+   EL +  
Sbjct: 193 VMAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHR 252

Query: 185 IRVNSVSPGAIGTPLLRNAL-----GFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLC 244
           IRVN +SP AI T  + + +     G  +  L ++V+S+ +L G V    DVA AA+YL 
Sbjct: 253 IRVNCISPFAITTSFVMDEMRQIYPGVDDSRLIQIVQSTGVLNGEVCEPTDVANAAVYLA 312

Query: 245 SDESRVISGHNLVVDGGYST 260
           SD+S+ ++GHNLVVDGG++T
Sbjct: 313 SDDSKYVNGHNLVVDGGFTT 330

BLAST of Lag0012792 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 228.0 bits (580), Expect = 9.1e-60
Identity = 119/254 (46.85%), Postives = 170/254 (66.93%), Query Frame = 0

Query: 6   STKRLEGKVAVITGAASGFGKSTARLFVKHGAKVIVADVQDDLGRALCEELGSEKTASYV 65
           S KRL+GK+ +ITG ASG G  + RLF +HGA+V++ DVQD+LG+ +   +G +K ASY 
Sbjct: 2   SGKRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDK-ASYY 61

Query: 66  HCDVTIDSDVKNAVDFAVERFGKLDIMYNNAGITGEMDPTILGTDGENFKRVFEVNVYGG 125
           HCDVT +++V+NAV F VE++GKLD++++NAG+  E   +IL  +     R   +N+ G 
Sbjct: 62  HCDVTNETEVENAVKFTVEKYGKLDVLFSNAGVI-EPFVSILDLNLNELDRTIAINLRGT 121

Query: 126 FLGAKHAARVMIPRR-SGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFG 185
               KHAAR M+ +   G I+ T+SVA+  +G +PH Y  SKH ++GL+++    LG++G
Sbjct: 122 AAFIKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYG 181

Query: 186 IRVNSVSPGAIGTPLLRNALGFTEEMLEEVVRSSAILKGAVPTAEDVAEAALYLCSDESR 245
           IRVN V+P  + TPL+ N       ++E+   +SA LKG V  A  VAEAAL+L SDES 
Sbjct: 182 IRVNGVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESA 241

Query: 246 VISGHNLVVDGGYS 259
            +SG NL VDGGYS
Sbjct: 242 YVSGQNLAVDGGYS 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6604329.11.3e-13791.97hypothetical protein SDJN03_04938, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022979096.13.9e-13791.61secoisolariciresinol dehydrogenase-like [Cucurbita maxima][more]
XP_023543891.16.6e-13791.24secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo][more]
XP_022925996.18.6e-13790.88secoisolariciresinol dehydrogenase-like [Cucurbita moschata][more]
XP_038882752.11.8e-13490.15secoisolariciresinol dehydrogenase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q94KL82.0e-7552.65Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
A3F5F07.5e-7552.08Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ... [more]
Q7FAE12.7e-7255.34Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ... [more]
Q94KL71.3e-7155.69Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
F1SWA01.6e-6953.08Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IPT51.9e-13791.61secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111478... [more]
A0A6J1EDN54.2e-13790.88secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1BXW71.6e-13389.05momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111005735 PE=4 ... [more]
A0A5A7SIU85.8e-13188.00Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CHD55.8e-13188.00secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103500923 ... [more]
Match NameE-valueIdentityDescription
AT3G26770.17.7e-6750.58NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G51680.11.0e-6649.64NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G52340.12.7e-6449.45NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G03140.12.3e-6349.23NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47140.19.1e-6046.85NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 87..98
score: 57.66
coord: 136..152
score: 31.47
coord: 221..241
score: 30.41
coord: 162..181
score: 38.45
coord: 183..200
score: 47.66
coord: 14..31
score: 44.9
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 142..150
score: 44.98
coord: 87..98
score: 50.98
coord: 162..181
score: 33.69
NoneNo IPR availableGENE3D3.40.50.720coord: 1..273
e-value: 1.8E-85
score: 288.3
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 19..258
e-value: 1.7E-69
score: 233.9
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 9..260
e-value: 1.5E-26
score: 90.9
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 16..209
e-value: 2.0E-21
score: 74.2
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 4..268
NoneNo IPR availablePANTHERPTHR43180:SF45SECOISOLARICIRESINOL DEHYDROGENASE-RELATEDcoord: 4..268
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 11..258

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012792.1Lag0012792.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0055041 cyclopentanol dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity