Lag0012791 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012791
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionIAA-amino acid hydrolase ILR1
Locationchr1: 44388111 .. 44390623 (-)
RNA-Seq ExpressionLag0012791
SyntenyLag0012791
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGGCGGCGGCATGGACTTCAAGTTGTTTTGTTGGATGCTGGTCTTTGGGGTGGTCTTGTGGCGGCCGTCGGCGGCGGAGCACGGCGGAGATGGGTCGGAGCTCCACTCACTGACCCGGAAGTTGTTGGGTTTGGCGAGGGAAGGGGAGTTCTTGGAGTGGATAAAAGGAGTCAGAAGGAGAATCCATGAGTATCCAGAGCTGGGATTTGAAGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTCGACTCGCTTGGAATCAGTTACCAGTGGCCTGTTGCCAAGACTGGAGTGGTGGCTTCCATTAAAGGGGCTTCTGGTTCTTCTTCTAGACCTGTTTTTGCTCTCAGAGCAGACATGGATGCTCTTCCTCTTCAGGTACTATCTCTCTCTCTCTCTCTCTCTCTATCTCTTTATATCACTTTCTCTCTCCTCATCTTCATCATCATCATCTTCTTCTTCTTTCATCATATATGATGTTGACAAAAGTCACATGCATGGAGATTGTTGGTTATGCTTTTCCTAAGTGAAGCATAAGTACAAATCATGTCTTTTCAATTCCAAAAATGCAATTTAATGTGTATTTGTATTCCTCTCTAAGATAAGGAATAAAACAAGGTGGTTATATTTTAGAACATTTGGATTTTTTGAAGTCTTAGCCAAATTTCAAAAATTAAAAAAAGGGCTATATATATATAATTTTATCTATAATCTGTGAGGGGGTGTGGTTGAAAATGAATAAAATTTAGTATATAAAAGATGAAACTGCAAATTTTTGAAAGGACATAGATGAAACCGGAAAATGGCATATAGATCAACGACAATAAAAAAAATTAATTTTAAAAAAGTACCTTTGGAAAATATAAAGCAACCAAGGGTCTAAAAATATTTACATTTTGATAAATATGGGGAGGAAAGAAAAATGAGATGATTTCATACATTTCCAGACCAGACTAGCATATCAAGAAACTGTTTCAACTAAACTTGTTGGCTTCCTATCCTCGCTCCAAAGGGCCGATATTCAATGGGTTACAAAAGTTCAATTACTTCCTGGCAAATTGAAATTCAACCTTGCTAATTGTTGTTTTCAATAGAGATGATCATGCCAGGGAGTGCAAAAACTGAGTACAATTGATGAGCAGTTTCAGATTCTCCAAGCTTAATAAAAAGTTTATTCTCTTCATATAATTTTGAAGGAACTGGTAGAATGGGAATTCAAAAGCAAGGTAGAAGGTAAAATGCATGCCTGTGGTCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTGCTACAGAGTAAAAGAGATAAACTGAAGGTAAAAATTTTCAGACTTATAATTGTAAATCCTGTTTGTTTGATTCAGTTAACAGAGGAGGGGACTGACCAGGATTGTTTGTATACGTTGTTATTTGAAGGGAACTGTGAAGCTAGTTTTCCAGCCTGGAGAAGAGTGTAATGGGGCTTATCAAATGCTACAAGAAGATGCCCTTGATGATATAGATGGAATCTTTGCATTGCACGTCCTGCCCTCCCTACCAACTGGTGTCATAGCCTCTAGGCCGGGTCCAGTATGTGCTGGAGCAGGACACTTTTCAGCCTTGATCCGAGGAAAGGGTGGACATGCAGCAACCCCACATAAGACGAAGGACCCAGTTCTGGCAACAGCTTTCATAATTCAAGCTCTTCAACAGATCGTATCTCGTGAGACAGATCCTCTTGAGGCCGGAGTAAGTTTTGCGCCCTCTAAATGAAGTCCCTAATCTTCTTAATTCGTTAACATCATAAGAACAGAATAGCAGAAGATAAAATTATCAACTACTCAACAAGATTCAACAATTTAGTCCTAACAACAGCTAAAAAAGTAATGCATAACCAGGCAACTTGAAGTTTGATCGTAGTAAACTAATAATCAGCAATGTAGGTGGTGACGGTTGCATTTGTTGAGGGAGGGCAAGCAGAAAATGTAGTTCCCGAGACCGTGAAAGTCGGGGGAACTTTTAGGAGCTTGTCTCCTGAGGGTCTTTCTTACCTTAAGGAAAGGATCAGAGAGGTATGAAATGCACTACGGTTGACAAACTTAACAAATTCCTATATTAAATTTGAAGCCAGGCAAAATTTAATTCCTCCTCAACAGGTCATCAACACACAAGCAGTAGTGCACCATTGTTATGCTTCAGTGGAGTTTATGGAGGATACACCAGTAATGGTTAACAATATAGCATTGTTTGAACATGCAAATAGAGTAGGAAATAGCTTACTTGGAGAACCCAATGTGCAACTCCTTCCATGGACCATGGGAGCTGAAGATTTTGGCTTCTTCTCACAGAGGATTGCAGCTACCATATATGGGATTGGGATAAGGAATGAGACTCTGAGATCAAACCGGCCATTGCACTCACCGCACTTTGTCCTAGATGAGGAGGCCCTTCCAATAGGAGCAGCTCTTCATGCTGCTGTGGCCATGTCTTATTTGGAACACAATTCAGTTTTCAGCGATTAA

mRNA sequence

ATGGCAGGCGGCGGCATGGACTTCAAGTTGTTTTGTTGGATGCTGGTCTTTGGGGTGGTCTTGTGGCGGCCGTCGGCGGCGGAGCACGGCGGAGATGGGTCGGAGCTCCACTCACTGACCCGGAAGTTGTTGGGTTTGGCGAGGGAAGGGGAGTTCTTGGAGTGGATAAAAGGAGTCAGAAGGAGAATCCATGAGTATCCAGAGCTGGGATTTGAAGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTCGACTCGCTTGGAATCAGTTACCAGTGGCCTGTTGCCAAGACTGGAGTGGTGGCTTCCATTAAAGGGGCTTCTGGTTCTTCTTCTAGACCTGTTTTTGCTCTCAGAGCAGACATGGATGCTCTTCCTCTTCAGGAACTGGTAGAATGGGAATTCAAAAGCAAGGTAGAAGGTAAAATGCATGCCTGTGGTCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTGCTACAGAGTAAAAGAGATAAACTGAAGGGAACTGTGAAGCTAGTTTTCCAGCCTGGAGAAGAGTGTAATGGGGCTTATCAAATGCTACAAGAAGATGCCCTTGATGATATAGATGGAATCTTTGCATTGCACGTCCTGCCCTCCCTACCAACTGGTGTCATAGCCTCTAGGCCGGGTCCAGTATGTGCTGGAGCAGGACACTTTTCAGCCTTGATCCGAGGAAAGGGTGGACATGCAGCAACCCCACATAAGACGAAGGACCCAGTTCTGGCAACAGCTTTCATAATTCAAGCTCTTCAACAGATCGTATCTCGTGAGACAGATCCTCTTGAGGCCGGAGTGGTGACGGTTGCATTTGTTGAGGGAGGGCAAGCAGAAAATGTAGTTCCCGAGACCGTGAAAGTCGGGGGAACTTTTAGGAGCTTGTCTCCTGAGGGTCTTTCTTACCTTAAGGAAAGGATCAGAGAGGTCATCAACACACAAGCAGTAGTGCACCATTGTTATGCTTCAGTGGAGTTTATGGAGGATACACCAGTAATGGTTAACAATATAGCATTGTTTGAACATGCAAATAGAGTAGGAAATAGCTTACTTGGAGAACCCAATGTGCAACTCCTTCCATGGACCATGGGAGCTGAAGATTTTGGCTTCTTCTCACAGAGGATTGCAGCTACCATATATGGGATTGGGATAAGGAATGAGACTCTGAGATCAAACCGGCCATTGCACTCACCGCACTTTGTCCTAGATGAGGAGGCCCTTCCAATAGGAGCAGCTCTTCATGCTGCTGTGGCCATGTCTTATTTGGAACACAATTCAGTTTTCAGCGATTAA

Coding sequence (CDS)

ATGGCAGGCGGCGGCATGGACTTCAAGTTGTTTTGTTGGATGCTGGTCTTTGGGGTGGTCTTGTGGCGGCCGTCGGCGGCGGAGCACGGCGGAGATGGGTCGGAGCTCCACTCACTGACCCGGAAGTTGTTGGGTTTGGCGAGGGAAGGGGAGTTCTTGGAGTGGATAAAAGGAGTCAGAAGGAGAATCCATGAGTATCCAGAGCTGGGATTTGAAGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTCGACTCGCTTGGAATCAGTTACCAGTGGCCTGTTGCCAAGACTGGAGTGGTGGCTTCCATTAAAGGGGCTTCTGGTTCTTCTTCTAGACCTGTTTTTGCTCTCAGAGCAGACATGGATGCTCTTCCTCTTCAGGAACTGGTAGAATGGGAATTCAAAAGCAAGGTAGAAGGTAAAATGCATGCCTGTGGTCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTGCTACAGAGTAAAAGAGATAAACTGAAGGGAACTGTGAAGCTAGTTTTCCAGCCTGGAGAAGAGTGTAATGGGGCTTATCAAATGCTACAAGAAGATGCCCTTGATGATATAGATGGAATCTTTGCATTGCACGTCCTGCCCTCCCTACCAACTGGTGTCATAGCCTCTAGGCCGGGTCCAGTATGTGCTGGAGCAGGACACTTTTCAGCCTTGATCCGAGGAAAGGGTGGACATGCAGCAACCCCACATAAGACGAAGGACCCAGTTCTGGCAACAGCTTTCATAATTCAAGCTCTTCAACAGATCGTATCTCGTGAGACAGATCCTCTTGAGGCCGGAGTGGTGACGGTTGCATTTGTTGAGGGAGGGCAAGCAGAAAATGTAGTTCCCGAGACCGTGAAAGTCGGGGGAACTTTTAGGAGCTTGTCTCCTGAGGGTCTTTCTTACCTTAAGGAAAGGATCAGAGAGGTCATCAACACACAAGCAGTAGTGCACCATTGTTATGCTTCAGTGGAGTTTATGGAGGATACACCAGTAATGGTTAACAATATAGCATTGTTTGAACATGCAAATAGAGTAGGAAATAGCTTACTTGGAGAACCCAATGTGCAACTCCTTCCATGGACCATGGGAGCTGAAGATTTTGGCTTCTTCTCACAGAGGATTGCAGCTACCATATATGGGATTGGGATAAGGAATGAGACTCTGAGATCAAACCGGCCATTGCACTCACCGCACTTTGTCCTAGATGAGGAGGCCCTTCCAATAGGAGCAGCTCTTCATGCTGCTGTGGCCATGTCTTATTTGGAACACAATTCAGTTTTCAGCGATTAA

Protein sequence

MAGGGMDFKLFCWMLVFGVVLWRPSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGNSLLGEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNSVFSD
Homology
BLAST of Lag0012791 vs. NCBI nr
Match: XP_022925994.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata] >KAG6604330.1 IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034481.1 IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 843.6 bits (2178), Expect = 7.8e-241
Identity = 417/438 (95.21%), Postives = 427/438 (97.49%), Query Frame = 0

Query: 1   MAGGGMDFKLFCWMLVFGVVLWRPSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVR 60
           MAGGGM FKLFCWML+FGV LWRPSAAEHGGDGSELHSLT+KL+GLAREG+F+EWIKGVR
Sbjct: 1   MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 60

Query: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRA 120
           RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASI GAS SSS PVFALRA
Sbjct: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA 120

Query: 121 DMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPG 180
           DMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSKR KLKGTVKLVFQPG
Sbjct: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG 180

Query: 181 EECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240
           EECNGAYQMLQEDALD+IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA
Sbjct: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240

Query: 241 ATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTF 300
           A PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFV+GGQAENVVPETVKVGGTF
Sbjct: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300

Query: 301 RSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGNSLLG 360
           RSLSPEGLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGNSLLG
Sbjct: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG 360

Query: 361 EPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGAA 420
           E NVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETL S+RPLHSPHFVLDEEALPIGAA
Sbjct: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420

Query: 421 LHAAVAMSYLEHNSVFSD 439
           LHAAVAMSYLEHNSVFSD
Sbjct: 421 LHAAVAMSYLEHNSVFSD 438

BLAST of Lag0012791 vs. NCBI nr
Match: XP_023543889.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 840.1 bits (2169), Expect = 8.6e-240
Identity = 414/436 (94.95%), Postives = 424/436 (97.25%), Query Frame = 0

Query: 3   GGGMDFKLFCWMLVFGVVLWRPSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVRRR 62
           GGGMDFKLFCWML FGV LWRPSAA HGGDGSELHSLT+KL+GLAREG+F+EWIKGVRRR
Sbjct: 4   GGGMDFKLFCWMLFFGVALWRPSAAGHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRR 63

Query: 63  IHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRADM 122
           IHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASI GAS SSS PVFALRADM
Sbjct: 64  IHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSTPVFALRADM 123

Query: 123 DALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEE 182
           DALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSKR KLKGTVKLVFQPGEE
Sbjct: 124 DALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEE 183

Query: 183 CNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAT 242
           CNGAYQMLQEDALD+IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA 
Sbjct: 184 CNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAA 243

Query: 243 PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTFRS 302
           PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFV+GGQAENVVPETVKVGGTFRS
Sbjct: 244 PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRS 303

Query: 303 LSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGNSLLGEP 362
           LSPEGLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGNSLLGE 
Sbjct: 304 LSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGNSLLGES 363

Query: 363 NVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGAALH 422
           NVQLLPWTMGAEDFGFFSQRIAATIYGIG+RNETL S+RPLHSPHFVLDEEALPIGAALH
Sbjct: 364 NVQLLPWTMGAEDFGFFSQRIAATIYGIGVRNETLGSSRPLHSPHFVLDEEALPIGAALH 423

Query: 423 AAVAMSYLEHNSVFSD 439
           AAVAMSYLEHNSVFSD
Sbjct: 424 AAVAMSYLEHNSVFSD 439

BLAST of Lag0012791 vs. NCBI nr
Match: XP_022979169.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita maxima])

HSP 1 Score: 837.8 bits (2163), Expect = 4.3e-239
Identity = 416/438 (94.98%), Postives = 426/438 (97.26%), Query Frame = 0

Query: 1   MAGGGMDFKLFCWMLVFGVVLWRPSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVR 60
           MAGGGMDFKLFC ML FGV LWRPSAAEHGGDGS+LHSLT+KL+GLAREGEF+EWIKGVR
Sbjct: 1   MAGGGMDFKLFCSMLFFGVALWRPSAAEHGGDGSDLHSLTQKLMGLAREGEFMEWIKGVR 60

Query: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRA 120
           RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASI GAS SSS PVFALRA
Sbjct: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSTPVFALRA 120

Query: 121 DMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPG 180
           DMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLL+SKR KLKGTVKLVFQPG
Sbjct: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLRSKRLKLKGTVKLVFQPG 180

Query: 181 EECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240
           EECNGAYQMLQEDALD+IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA
Sbjct: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240

Query: 241 ATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTF 300
           A PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFV+GGQAENVVPETVKVGGTF
Sbjct: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300

Query: 301 RSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGNSLLG 360
           RSLSPEGLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGNSLLG
Sbjct: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGNSLLG 360

Query: 361 EPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGAA 420
           E NVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETL S+RPLHSPHFVLDEEALPIGAA
Sbjct: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420

Query: 421 LHAAVAMSYLEHNSVFSD 439
           LHAAVAMSYLEHNSVFSD
Sbjct: 421 LHAAVAMSYLEHNSVFSD 438

BLAST of Lag0012791 vs. NCBI nr
Match: XP_022962856.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata])

HSP 1 Score: 828.2 bits (2138), Expect = 3.4e-236
Identity = 411/437 (94.05%), Postives = 423/437 (96.80%), Query Frame = 0

Query: 3   GGGMDFKLFCWMLVFGVVLWRPSAA--EHGGDGSELHSLTRKLLGLAREGEFLEWIKGVR 62
           GGGM+FKLFCWMLVFGV LWRPSA+  EHGGDG ELHSLTRKLLGLAREGEFLEWIKGVR
Sbjct: 26  GGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVR 85

Query: 63  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGA----SGSSSRPVF 122
           RRIHEYPE+GFEEYRTSQLVRSELD LGISYQWPVAKTGVVASIKGA    S SSSRPVF
Sbjct: 86  RRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVF 145

Query: 123 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLV 182
           ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK +KLKGTVKLV
Sbjct: 146 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLV 205

Query: 183 FQPGEECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGK 242
           FQPGEECNGAYQ+LQEDALDDIDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGK
Sbjct: 206 FQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGK 265

Query: 243 GGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKV 302
           GGHAA PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFV+GGQA+NVVPETVKV
Sbjct: 266 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKV 325

Query: 303 GGTFRSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGN 362
           GGTFRSLSP+GLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNN+ALFEHANRVGN
Sbjct: 326 GGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGN 385

Query: 363 SLLGEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALP 422
           SLLGE NVQLLPWTMGAEDFGF SQ+IAATIYGIGIRN+TLRSNRPLHSPHFVLDEEALP
Sbjct: 386 SLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALP 445

Query: 423 IGAALHAAVAMSYLEHN 434
           IGAALHAAVAMSYLEHN
Sbjct: 446 IGAALHAAVAMSYLEHN 462

BLAST of Lag0012791 vs. NCBI nr
Match: XP_023517717.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.1 bits (2130), Expect = 2.9e-235
Identity = 411/446 (92.15%), Postives = 424/446 (95.07%), Query Frame = 0

Query: 3   GGGMDFKLFCWMLVFGVVLWRPSAA--EHGGDGSELHSLTRKLLGLAREGEFLEWIKGVR 62
           GGGM+FKLFCWMLVFGVVLWRPSA+  EHGGDG ELHSLTR+LLGLAREGEFLEWIKGVR
Sbjct: 5   GGGMEFKLFCWMLVFGVVLWRPSASEVEHGGDGLELHSLTRRLLGLAREGEFLEWIKGVR 64

Query: 63  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGA------------- 122
           RRIHEYPE+GFEEYRTSQLVRSELD LGISYQWPVAKTGVVASIKGA             
Sbjct: 65  RRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSSSSS 124

Query: 123 SGSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRD 182
           S SSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK +
Sbjct: 125 SSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIE 184

Query: 183 KLKGTVKLVFQPGEECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAG 242
           KLKGTVKLVFQPGEECNGAYQ+LQEDALDDIDGIFALHVLP+LPTGVIASRPGPVCAGAG
Sbjct: 185 KLKGTVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAG 244

Query: 243 HFSALIRGKGGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAE 302
           HFSALIRGKGGHAA PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFV+GGQA+
Sbjct: 245 HFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAD 304

Query: 303 NVVPETVKVGGTFRSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIAL 362
           NVVPETVKVGGTFRSLSP+GLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNN+AL
Sbjct: 305 NVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVAL 364

Query: 363 FEHANRVGNSLLGEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPH 422
           FEHANRVGNSLLGE NVQLLPWTMGAEDFGF SQ+IAATIYGIGIRN+TLRSNRPLHSPH
Sbjct: 365 FEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPH 424

Query: 423 FVLDEEALPIGAALHAAVAMSYLEHN 434
           FVLDEEALPIGAALHAAVAMSYLEHN
Sbjct: 425 FVLDEEALPIGAALHAAVAMSYLEHN 450

BLAST of Lag0012791 vs. ExPASy Swiss-Prot
Match: P54968 (IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2)

HSP 1 Score: 507.3 bits (1305), Expect = 1.7e-142
Identity = 253/435 (58.16%), Postives = 328/435 (75.40%), Query Frame = 0

Query: 6   MDFKLFCWMLVFGVVLWRPSAAEHGG--DGSELHSLTRKLLGLAREGEFLEWIKGVRRRI 65
           MDF    + ++F    + P  +  G    GS L SL R +L  A++ EF EW++G+RR+I
Sbjct: 1   MDFS-GSFFVIFVTFFFLPPLSSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRKI 60

Query: 66  HEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRADMD 125
           HE PE GF+E++TSQLVR ELDSLG+ Y++PVAKTGVVA I    GS S+PVF LRADMD
Sbjct: 61  HENPETGFQEFKTSQLVRDELDSLGVKYKYPVAKTGVVAWI----GSCSKPVFGLRADMD 120

Query: 126 ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEE- 185
           ALPLQELVEWE KSKV+GKMHACGHD+HVAMLLGAA+LLQ+ +  +KGTVKLVFQPGEE 
Sbjct: 121 ALPLQELVEWESKSKVDGKMHACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGEEG 180

Query: 186 CNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAT 245
             GAY+ML+++ LDD+DGI ++HV PS+P+G I SRPG V AGAG F+  + G+G HAAT
Sbjct: 181 YAGAYEMLKDEILDDLDGILSVHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHAAT 240

Query: 246 PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTFRS 305
           PH +KDPVLA +  + ALQQIVSRE DPLEAGVVTV ++EGG A+NV+P++ K GGTFRS
Sbjct: 241 PHFSKDPVLAASSAVVALQQIVSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTFRS 300

Query: 306 LSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDT----PVMVNNIALFEHANRVGNSL 365
           LS +GL +++ RI+E+   QA V+ C A V F E      PVM N+  L+EH  +V  ++
Sbjct: 301 LSNDGLLFIQRRIKEISEAQASVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAEAM 360

Query: 366 LGEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIG 425
           +G+ N    P TMG EDF FF+Q+  A I+ +G++NETL + +PLHSP+F +DEEALP+G
Sbjct: 361 IGKNNFHDFPVTMGGEDFSFFTQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVG 420

Query: 426 AALHAAVAMSYLEHN 434
           AALHAA+A+SYL+ +
Sbjct: 421 AALHAAMAVSYLDEH 430

BLAST of Lag0012791 vs. ExPASy Swiss-Prot
Match: Q851L5 (IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL3 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.2e-135
Identity = 242/408 (59.31%), Postives = 308/408 (75.49%), Query Frame = 0

Query: 38  SLTRKLLGLAREGEFLEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAK 97
           +L R+LL  AR  EF  W++G+RRRIH++PEL F+E+RTS LVR+ELD+LG++Y WPVA+
Sbjct: 8   TLGRELLEAARAPEFAGWLRGLRRRIHQHPELAFQEHRTSALVRAELDALGVAYVWPVAQ 67

Query: 98  TGVVASIKGASGSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLG 157
           TGVVA++ GA+G    PVF LRADMDALP+QE+VEWEFKS  +GKMHACGHD HVAMLLG
Sbjct: 68  TGVVATVVGAAGPG--PVFGLRADMDALPIQEMVEWEFKSLEDGKMHACGHDVHVAMLLG 127

Query: 158 AARLLQSKRDKLKGTVKLVFQPGEE-CNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIA 217
           AA+LLQS+RD   G VKLVFQP EE   G Y +L+E A+DD+ GIF +HV   LP GV+A
Sbjct: 128 AAKLLQSRRDHFNGKVKLVFQPAEEGYAGGYYVLEEGAVDDVQGIFGMHVDAGLPAGVVA 187

Query: 218 SRPGPVCAGAGHFSALIRGKGGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVV 277
           SRPGP  AG+  F+A I GKGGHAA PH   DP++A +  + +LQQIV+RETDPL+  VV
Sbjct: 188 SRPGPFLAGSARFTATINGKGGHAAAPHHAVDPIVAVSSAVLSLQQIVARETDPLQGAVV 247

Query: 278 TVAFVEGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINTQAVVHHCYASVEFME 337
           +V  ++GG+A NV+PE+V +GGT RS++ +G+SYL +RIREVI  QA V+ C A+V+FME
Sbjct: 248 SVTTIKGGEAFNVIPESVTLGGTLRSMTTDGMSYLMKRIREVIEGQAAVNRCTAAVDFME 307

Query: 338 DT----PVMVNNIALFEHANRVGNSLLGEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGI 397
           D     P  VN+  ++ HA  V  S+LGE NV+L P  MGAEDFGF++QRI A  +GIG+
Sbjct: 308 DKLPPYPATVNDEEMYAHAKAVAESMLGEANVKLSPQGMGAEDFGFYAQRIPAAFFGIGV 367

Query: 398 RN------ETLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNS 435
            N      ET   N+ LHSPHFV+DEEALP+GAA HAAVA+ YL  N+
Sbjct: 368 GNDGGGMAETTTKNQ-LHSPHFVVDEEALPVGAAFHAAVAIEYLNKNA 412

BLAST of Lag0012791 vs. ExPASy Swiss-Prot
Match: Q8H3C9 (IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL7 PE=2 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 2.1e-132
Identity = 237/417 (56.83%), Postives = 298/417 (71.46%), Query Frame = 0

Query: 24  PSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPELGFEEYRTSQLVRSE 83
           P+    GG G E       LL  AR   F  W++G+RR IH +PEL FEE RTS+LVR+E
Sbjct: 41  PAGGGGGGSGGE-------LLSAARAPGFAAWLRGLRRSIHRHPELAFEEVRTSELVRAE 100

Query: 84  LDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRADMDALPLQELVEWEFKSKVEGKM 143
           LD++G+ Y+WPVA+TGVVA+I G  G+ +  VFALRADMDALPLQELV+WE KS+  GKM
Sbjct: 101 LDAIGVPYEWPVARTGVVATIAGGDGAGAGTVFALRADMDALPLQELVDWEHKSEESGKM 160

Query: 144 HACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEE-CNGAYQMLQEDALDDIDGIF 203
           HACGHD+H  MLLGAA+LLQS++D LKGTVKLVFQP EE   GA  +LQE  LDD+  IF
Sbjct: 161 HACGHDAHTTMLLGAAKLLQSQKDDLKGTVKLVFQPAEEGYAGARYVLQEGVLDDVSAIF 220

Query: 204 ALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAATPHKTKDPVLATAFIIQALQQ 263
            LHV P +  G + SRPGP  A +G F A I GKGGHAA PH   DP+L  +  I +LQQ
Sbjct: 221 GLHVDPRIQVGTVTSRPGPFLAASGRFLATITGKGGHAAGPHNAVDPILTASSAIVSLQQ 280

Query: 264 IVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINTQ 323
           IV+RETDPLEA V++V F++GG A NV+PE+V  GGTFRSL+ EGLSYLK+RI+E++   
Sbjct: 281 IVARETDPLEAAVISVTFMKGGDAYNVIPESVSFGGTFRSLTSEGLSYLKKRIKEIVEAH 340

Query: 324 AVVHHCYASVEFMEDT----PVMVNNIALFEHANRVGNSLLGEPNVQLLPWTMGAEDFGF 383
           A VH C A+V+FME+     P  VN+  ++ HA  V   +LGE  V++    MG+EDF F
Sbjct: 341 ATVHRCTATVDFMEEERIPYPATVNDEGMYRHARAVAVDVLGEDGVKVGTPFMGSEDFAF 400

Query: 384 FSQRIAATIYGIGIRNE-TLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNS 435
           ++QR  A  + IG+ NE T+R   PLHSPHFV+DE+ LP+GAALHAAVAM YL  ++
Sbjct: 401 YAQRFPAAFFMIGVGNETTMRKVYPLHSPHFVVDEDVLPVGAALHAAVAMEYLNKHA 450

BLAST of Lag0012791 vs. ExPASy Swiss-Prot
Match: Q851L6 (IAA-amino acid hydrolase ILR1-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL4 PE=2 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 3.6e-132
Identity = 236/410 (57.56%), Postives = 303/410 (73.90%), Query Frame = 0

Query: 38  SLTRKLLGLAREGEFLEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAK 97
           +L R+LL  AR  EF  W++G+RRRIH++PEL F+E+RTS LVR+ELD+LG++Y WP+A+
Sbjct: 4   TLGRELLEAARAPEFAGWLRGLRRRIHQHPELAFQEHRTSALVRAELDALGVAYVWPIAQ 63

Query: 98  TGVVASIKGASGSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLG 157
           TGVVA++ GA+G    PVFALRADMDALP+QE+VEWEFKS  +GKMHACGHD+HVAMLL 
Sbjct: 64  TGVVATVAGAAGPG--PVFALRADMDALPIQEMVEWEFKSLEDGKMHACGHDAHVAMLLV 123

Query: 158 AARLLQSKRDKLKGTVKLVFQPGEECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIAS 217
           AA+LLQS+RD   G VKLVFQP E   G Y +L+E  LDD   IFA+HV   LP GV+ S
Sbjct: 124 AAKLLQSRRDHFNGKVKLVFQPAEGGAGGYHVLKEGVLDDTQTIFAVHVATDLPAGVVGS 183

Query: 218 RPGPVCAGAGHFSALIRGKGGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVT 277
           RPGP  AG+  F+A I GKGGHAA PH   DP++A +  + +LQQIV+RET+PL+  VV+
Sbjct: 184 RPGPFLAGSARFTATITGKGGHAAEPHLAVDPIVAASSAVLSLQQIVARETNPLQGAVVS 243

Query: 278 VAFVEGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMED 337
           V  ++GG+A NV+PE+V +GGT RS++ +GLSYL  RIREVI  QA V+ C A+V+FMED
Sbjct: 244 VTTIKGGEAFNVIPESVTLGGTLRSMTTDGLSYLMNRIREVIEGQAAVNRCTAAVDFMED 303

Query: 338 T----PVMVNNIALFEHANRVGNSLLGEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIR 397
                P  VN+  ++ HA  V  S+LGE NV + P  MGAEDFGF++QRI A  +GIG+ 
Sbjct: 304 KLRPYPATVNDEGMYAHAKAVAESMLGEANVTVSPMCMGAEDFGFYAQRIPAAFFGIGVG 363

Query: 398 N---------ETLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNS 435
           +         ET ++   LHSPHFV+DEEALP+GAA HAAVA+ YL  N+
Sbjct: 364 SNGNDGGGMAETTKNQ--LHSPHFVVDEEALPVGAAFHAAVAIEYLNKNA 409

BLAST of Lag0012791 vs. ExPASy Swiss-Prot
Match: Q8H3C8 (IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL8 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 4.4e-130
Identity = 229/404 (56.68%), Postives = 300/404 (74.26%), Query Frame = 0

Query: 36  LHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPV 95
           L +L   LL  A    F  W+ G+RRRIH+ PEL F+E RTS+LVR+ELD++G+ Y WPV
Sbjct: 37  LKALGEDLLAAAGAAGFAGWLSGLRRRIHQRPELAFQEVRTSELVRAELDAIGVPYAWPV 96

Query: 96  AKTGVVASIKGASGSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAML 155
           A+TGVVA+I G +G+   PV ALRADMDALPLQELV+WEFKS+ +GKMHACGHD+HV ML
Sbjct: 97  ARTGVVATIDGGAGAG--PVVALRADMDALPLQELVDWEFKSQEKGKMHACGHDAHVTML 156

Query: 156 LGAARLLQSKRDKLKGTVKLVFQPGEECN-GAYQMLQEDALDDIDGIFALHVLPSLPTGV 215
           LGAA+LLQS++D+LKGT+KLVFQP EE + GAY +L+   LDD+  IF LHV+P+LP GV
Sbjct: 157 LGAAKLLQSRKDELKGTIKLVFQPAEEGHAGAYHVLESGLLDDVSVIFGLHVIPNLPVGV 216

Query: 216 IASRPGPVCAGAGHFSALIRGKGGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAG 275
           +ASRPGP  + A  F+A   GKGGHA  PH   DPV+A +  + +LQQ+VSRETDPLEA 
Sbjct: 217 VASRPGPFMSAAARFAATFTGKGGHAGVPHDAVDPVVAVSSAVLSLQQLVSRETDPLEAA 276

Query: 276 VVTVAFVEGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINTQAVVHHCYASVEF 335
           VV++  ++GG A NV+PE+  +GGTFRS++ EGL+YL +RIRE+I  QA V+ C A+V+F
Sbjct: 277 VVSITILKGGDAYNVIPESASLGGTFRSMTDEGLAYLMKRIREIIEAQAGVNRCAAAVDF 336

Query: 336 MEDT----PVMVNNIALFEHANRVGNSLLGEPNVQLLPWTMGAEDFGFFSQRIAATIYGI 395
           +E+     P  VN+  ++ HA  V  ++LGE NV++   +MG EDF F+++R     + I
Sbjct: 337 LEEELRPYPATVNDDGMYGHAKAVAEAMLGEANVRVAARSMGGEDFAFYARRSPGAFFFI 396

Query: 396 GIRNET----LRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 431
           G+ NET      + RP+HSPHFVLDE ALP+GAALHAAVA+ YL
Sbjct: 397 GVGNETTMGPAAAVRPVHSPHFVLDERALPVGAALHAAVAIEYL 438

BLAST of Lag0012791 vs. ExPASy TrEMBL
Match: A0A6J1EDS0 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111433244 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.8e-241
Identity = 417/438 (95.21%), Postives = 427/438 (97.49%), Query Frame = 0

Query: 1   MAGGGMDFKLFCWMLVFGVVLWRPSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVR 60
           MAGGGM FKLFCWML+FGV LWRPSAAEHGGDGSELHSLT+KL+GLAREG+F+EWIKGVR
Sbjct: 1   MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 60

Query: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRA 120
           RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASI GAS SSS PVFALRA
Sbjct: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA 120

Query: 121 DMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPG 180
           DMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSKR KLKGTVKLVFQPG
Sbjct: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG 180

Query: 181 EECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240
           EECNGAYQMLQEDALD+IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA
Sbjct: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240

Query: 241 ATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTF 300
           A PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFV+GGQAENVVPETVKVGGTF
Sbjct: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300

Query: 301 RSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGNSLLG 360
           RSLSPEGLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGNSLLG
Sbjct: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG 360

Query: 361 EPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGAA 420
           E NVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETL S+RPLHSPHFVLDEEALPIGAA
Sbjct: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420

Query: 421 LHAAVAMSYLEHNSVFSD 439
           LHAAVAMSYLEHNSVFSD
Sbjct: 421 LHAAVAMSYLEHNSVFSD 438

BLAST of Lag0012791 vs. ExPASy TrEMBL
Match: A0A6J1IVY2 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111478889 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.1e-239
Identity = 416/438 (94.98%), Postives = 426/438 (97.26%), Query Frame = 0

Query: 1   MAGGGMDFKLFCWMLVFGVVLWRPSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVR 60
           MAGGGMDFKLFC ML FGV LWRPSAAEHGGDGS+LHSLT+KL+GLAREGEF+EWIKGVR
Sbjct: 1   MAGGGMDFKLFCSMLFFGVALWRPSAAEHGGDGSDLHSLTQKLMGLAREGEFMEWIKGVR 60

Query: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRA 120
           RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASI GAS SSS PVFALRA
Sbjct: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSTPVFALRA 120

Query: 121 DMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPG 180
           DMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLL+SKR KLKGTVKLVFQPG
Sbjct: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLRSKRLKLKGTVKLVFQPG 180

Query: 181 EECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240
           EECNGAYQMLQEDALD+IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA
Sbjct: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240

Query: 241 ATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTF 300
           A PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFV+GGQAENVVPETVKVGGTF
Sbjct: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300

Query: 301 RSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGNSLLG 360
           RSLSPEGLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGNSLLG
Sbjct: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGNSLLG 360

Query: 361 EPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGAA 420
           E NVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETL S+RPLHSPHFVLDEEALPIGAA
Sbjct: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420

Query: 421 LHAAVAMSYLEHNSVFSD 439
           LHAAVAMSYLEHNSVFSD
Sbjct: 421 LHAAVAMSYLEHNSVFSD 438

BLAST of Lag0012791 vs. ExPASy TrEMBL
Match: A0A6J1HEG5 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111463223 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.6e-236
Identity = 411/437 (94.05%), Postives = 423/437 (96.80%), Query Frame = 0

Query: 3   GGGMDFKLFCWMLVFGVVLWRPSAA--EHGGDGSELHSLTRKLLGLAREGEFLEWIKGVR 62
           GGGM+FKLFCWMLVFGV LWRPSA+  EHGGDG ELHSLTRKLLGLAREGEFLEWIKGVR
Sbjct: 26  GGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVR 85

Query: 63  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGA----SGSSSRPVF 122
           RRIHEYPE+GFEEYRTSQLVRSELD LGISYQWPVAKTGVVASIKGA    S SSSRPVF
Sbjct: 86  RRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVF 145

Query: 123 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLV 182
           ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK +KLKGTVKLV
Sbjct: 146 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLV 205

Query: 183 FQPGEECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGK 242
           FQPGEECNGAYQ+LQEDALDDIDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGK
Sbjct: 206 FQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGK 265

Query: 243 GGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKV 302
           GGHAA PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFV+GGQA+NVVPETVKV
Sbjct: 266 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKV 325

Query: 303 GGTFRSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGN 362
           GGTFRSLSP+GLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNN+ALFEHANRVGN
Sbjct: 326 GGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGN 385

Query: 363 SLLGEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALP 422
           SLLGE NVQLLPWTMGAEDFGF SQ+IAATIYGIGIRN+TLRSNRPLHSPHFVLDEEALP
Sbjct: 386 SLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALP 445

Query: 423 IGAALHAAVAMSYLEHN 434
           IGAALHAAVAMSYLEHN
Sbjct: 446 IGAALHAAVAMSYLEHN 462

BLAST of Lag0012791 vs. ExPASy TrEMBL
Match: A0A6J1BV66 (IAA-amino acid hydrolase ILR1-like 3 OS=Momordica charantia OX=3673 GN=LOC111005645 PE=3 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.0e-225
Identity = 393/431 (91.18%), Postives = 412/431 (95.59%), Query Frame = 0

Query: 10  LFCWMLVFGVVLWR-PSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPE 69
           LFCWM+  G+ L R  +AA  GGDGSELHSLTR LLG AREGEF+EWIKGVRRRIHEYPE
Sbjct: 11  LFCWMVFLGMALSRSAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYPE 70

Query: 70  LGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASG-SSSRPVFALRADMDALPL 129
           LGFEEYRTSQLVRSELDSLGISYQWPVA+TGVVASIKGA+  SSSRPVFALRADMDALPL
Sbjct: 71  LGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGAASLSSSRPVFALRADMDALPL 130

Query: 130 QELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAY 189
           QELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAY
Sbjct: 131 QELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECNGAY 190

Query: 190 QMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAATPHKTK 249
           +ML+++ALD IDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA PH TK
Sbjct: 191 KMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIPHMTK 250

Query: 250 DPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTFRSLSPEG 309
           DPVLATAFIIQALQQIVSRETDPLEAGVVT+AFVEGGQAEN+VPETVKVGGTFRSLSPEG
Sbjct: 251 DPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSLSPEG 310

Query: 310 LSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGNSLLGEPNVQLL 369
            SYLKERIREVINTQA VHHC+A+V+FMEDTPVMVNN+ALFEHAN VGNSLLGE NVQLL
Sbjct: 311 FSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESNVQLL 370

Query: 370 PWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGAALHAAVAM 429
           PWTMGAEDFGFFSQRIAATI+GIG+RN+TLRSNRPLHSPHFVLDEEALPIGAALHAAVAM
Sbjct: 371 PWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAM 430

Query: 430 SYLEHNSVFSD 439
           SYLEHNSVFSD
Sbjct: 431 SYLEHNSVFSD 441

BLAST of Lag0012791 vs. ExPASy TrEMBL
Match: A0A0A0KIR8 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454350 PE=3 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.7e-225
Identity = 391/438 (89.27%), Postives = 410/438 (93.61%), Query Frame = 0

Query: 2   AGGGMDFKLFCWMLVFGVVLWRPSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVRR 61
           AGGG  FK  CWM+VFG+ LWRP+A E+GGDGSELHSLT+ LLGLARE EF+EWIKGVRR
Sbjct: 3   AGGGTSFKSLCWMIVFGIALWRPAAGENGGDGSELHSLTQSLLGLAREREFMEWIKGVRR 62

Query: 62  RIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKG--ASGSSSRPVFALR 121
           RIHEYPELGFEEY+TSQLVRSELDSLGISY+WPVAKTGVVASI+G   S SSS PVF LR
Sbjct: 63  RIHEYPELGFEEYKTSQLVRSELDSLGISYRWPVAKTGVVASIRGDSVSSSSSTPVFGLR 122

Query: 122 ADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQP 181
           ADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAM+LGAARLLQS R+KLKGTVKLVFQP
Sbjct: 123 ADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMVLGAARLLQSIREKLKGTVKLVFQP 182

Query: 182 GEECNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGH 241
            EECNGAYQML++DALD IDGIFALHV PSLPTGVIASRPGPVCAGAGHFSALIRGKGGH
Sbjct: 183 AEECNGAYQMLKDDALDGIDGIFALHVQPSLPTGVIASRPGPVCAGAGHFSALIRGKGGH 242

Query: 242 AATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGT 301
           AATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFV+GGQAENVVPETVKVGGT
Sbjct: 243 AATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGT 302

Query: 302 FRSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNIALFEHANRVGNSLL 361
           FRSLSPEG SYLKERIREVI+TQA+VHHCYASV+FMEDTPVMVNN ALFEH NRVGNSLL
Sbjct: 303 FRSLSPEGFSYLKERIREVISTQAMVHHCYASVKFMEDTPVMVNNEALFEHVNRVGNSLL 362

Query: 362 GEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGA 421
           GE NVQLLPWTMGAEDFGFFSQRI ATIY IG  NETL SNRP+HSPHFVLDEEALPIGA
Sbjct: 363 GESNVQLLPWTMGAEDFGFFSQRIPATIYVIGTGNETLGSNRPVHSPHFVLDEEALPIGA 422

Query: 422 ALHAAVAMSYLEHNSVFS 438
           ALHAAVA +YLEHNSVFS
Sbjct: 423 ALHAAVATTYLEHNSVFS 440

BLAST of Lag0012791 vs. TAIR 10
Match: AT3G02875.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 507.3 bits (1305), Expect = 1.2e-143
Identity = 253/435 (58.16%), Postives = 328/435 (75.40%), Query Frame = 0

Query: 6   MDFKLFCWMLVFGVVLWRPSAAEHGG--DGSELHSLTRKLLGLAREGEFLEWIKGVRRRI 65
           MDF    + ++F    + P  +  G    GS L SL R +L  A++ EF EW++G+RR+I
Sbjct: 1   MDFS-GSFFVIFVTFFFLPPLSSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRKI 60

Query: 66  HEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRADMD 125
           HE PE GF+E++TSQLVR ELDSLG+ Y++PVAKTGVVA I    GS S+PVF LRADMD
Sbjct: 61  HENPETGFQEFKTSQLVRDELDSLGVKYKYPVAKTGVVAWI----GSCSKPVFGLRADMD 120

Query: 126 ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEE- 185
           ALPLQELVEWE KSKV+GKMHACGHD+HVAMLLGAA+LLQ+ +  +KGTVKLVFQPGEE 
Sbjct: 121 ALPLQELVEWESKSKVDGKMHACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGEEG 180

Query: 186 CNGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAT 245
             GAY+ML+++ LDD+DGI ++HV PS+P+G I SRPG V AGAG F+  + G+G HAAT
Sbjct: 181 YAGAYEMLKDEILDDLDGILSVHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHAAT 240

Query: 246 PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTFRS 305
           PH +KDPVLA +  + ALQQIVSRE DPLEAGVVTV ++EGG A+NV+P++ K GGTFRS
Sbjct: 241 PHFSKDPVLAASSAVVALQQIVSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTFRS 300

Query: 306 LSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDT----PVMVNNIALFEHANRVGNSL 365
           LS +GL +++ RI+E+   QA V+ C A V F E      PVM N+  L+EH  +V  ++
Sbjct: 301 LSNDGLLFIQRRIKEISEAQASVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAEAM 360

Query: 366 LGEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIG 425
           +G+ N    P TMG EDF FF+Q+  A I+ +G++NETL + +PLHSP+F +DEEALP+G
Sbjct: 361 IGKNNFHDFPVTMGGEDFSFFTQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVG 420

Query: 426 AALHAAVAMSYLEHN 434
           AALHAA+A+SYL+ +
Sbjct: 421 AALHAAMAVSYLDEH 430

BLAST of Lag0012791 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 383.3 bits (983), Expect = 2.7e-106
Identity = 199/400 (49.75%), Postives = 269/400 (67.25%), Query Frame = 0

Query: 36  LHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPV 95
           L  +  K L LA+  +F +W+ G+RRRIHE PELG+EE  TS+LVR+EL+ +G+SY++PV
Sbjct: 28  LSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPELGYEEVETSKLVRAELEKMGVSYKYPV 87

Query: 96  AKTGVVASIKGASGSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAML 155
           A TGVV    G  G+   P  ALRADMDAL +QE+VEWE KSKV GKMHACGHD+H  ML
Sbjct: 88  AVTGVV----GYVGTGHAPFVALRADMDALAMQEMVEWEHKSKVPGKMHACGHDAHTTML 147

Query: 156 LGAARLLQSKRDKLKGTVKLVFQPGEE-CNGAYQMLQEDALDDIDGIFALHVLPSLPTGV 215
           LGAA+LL+   ++L+GTV LVFQP EE   GA ++++   L+++  IF LHV   L  G 
Sbjct: 148 LGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVEAGVLENVSAIFGLHVTNQLALGQ 207

Query: 216 IASRPGPVCAGAGHFSALIRGKGGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAG 275
           ++SR GP+ AG+G F A I GKGGHAA P  T DP+LA + +I +LQ +VSRE DPL++ 
Sbjct: 208 VSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPILAASNVIVSLQHLVSREADPLDSQ 267

Query: 276 VVTVAFVEGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINTQAVVHHCYASVEF 335
           VVTVA  EGG A NV+P++V +GGTFR+ S +    LK+RI +VI  QA V+ C A+V+F
Sbjct: 268 VVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQLKKRIEQVITRQASVNMCNATVDF 327

Query: 336 MEDT----PVMVNNIALFEHANRVGNSLLGEPNVQLLPWTMGAEDFGFFSQRIAATIYGI 395
           +E+     P  VN+ AL +    V   +LG  N   +   MG+EDF F+ Q I      +
Sbjct: 328 IEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFYQQAIPGHFSFV 387

Query: 396 GIRNETLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 431
           G++N+        HSP+F ++EE LP GA+LHA++A  YL
Sbjct: 388 GMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATRYL 423

BLAST of Lag0012791 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 376.7 bits (966), Expect = 2.5e-104
Identity = 203/394 (51.52%), Postives = 263/394 (66.75%), Query Frame = 0

Query: 42  KLLGLAREGEFLEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVV 101
           ++L L  + + + W+K VRR IHE PEL FEEY TS+L+RSELD +GI Y++P+AKTG+ 
Sbjct: 75  EILRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIR 134

Query: 102 ASIKGASGSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARL 161
           A I    GS   P  A+RADMDALP+QE VEWE  SKV GKMHACGHD+HV MLLGAA +
Sbjct: 135 AWI----GSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHI 194

Query: 162 LQSKRDKLKGTVKLVFQPGEEC-NGAYQMLQEDALDDIDGIFALHVLPSLPTGVIASRPG 221
           L+++   LKGTV L+FQP EE  NGA  M+++ ALDD++ IFA+HV    PTGVI SR G
Sbjct: 195 LKAREHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSG 254

Query: 222 PVCAGAGHFSALIRGKGGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAF 281
           P+ AG G F A+I  +    A      + +LA +  + +LQ IVSRE  PL++ VV+V  
Sbjct: 255 PLLAGCGIFRAVITSEDSRGAA-----NLLLAASSAVISLQGIVSREASPLDSQVVSVTS 314

Query: 282 VEGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINTQAVVHHCYASVEFMEDT-- 341
            +GG + +V P+TV +GGTFR+ S     YLK+RI+EV+  Q  V  C A+V F E    
Sbjct: 315 FDGGHSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNA 374

Query: 342 --PVMVNNIALFEHANRVGNSLLGEPNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNET 401
             P   NN A + H  +V   LLG+ +  L P  MGAEDF F+S+ I A  Y IGIRNE 
Sbjct: 375 IYPPTTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEE 434

Query: 402 LRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 431
           L S    HSPHF++DE++LP+GAA+HAAVA  YL
Sbjct: 435 LGSVHIAHSPHFMIDEDSLPVGAAVHAAVAERYL 459

BLAST of Lag0012791 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 375.6 bits (963), Expect = 5.6e-104
Identity = 204/430 (47.44%), Postives = 282/430 (65.58%), Query Frame = 0

Query: 11  FCWMLVFGVVLWRPSAAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPELG 70
           FC ++ F ++L   ++       ++L  + +  L LA+  +F +W+ G+RRRIHE PELG
Sbjct: 3   FCKLVSFVLILHLLNSCLISCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELG 62

Query: 71  FEEYRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRADMDALPLQEL 130
           +EE  TS+LV++ELD +G+SY+ PVA TGV+    G  G+   P  ALRADMDALP+QE+
Sbjct: 63  YEEVETSKLVKTELDKMGVSYKNPVAVTGVI----GYVGTGHAPFVALRADMDALPIQEM 122

Query: 131 VEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEECN-GAYQM 190
           VEWE KSK+ GKMHACGHD+H  MLLGAA+LL+  +++L+GTV LVFQP EE   GA ++
Sbjct: 123 VEWEHKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKI 182

Query: 191 LQEDALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAATPHKTKDP 250
           ++   L+++  IF LHV   L  G ++SR G + AG+G F A I GKGGHAA P    DP
Sbjct: 183 VEAGVLENVGAIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDP 242

Query: 251 VLATAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTFRSLSPEGLS 310
           VLA + +I +LQ +VSRE DPL++ VVTVA  EG  A NV+P++V +GGTFR+L P+   
Sbjct: 243 VLAASNVILSLQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFE 302

Query: 311 YLKERIREVINTQAVVHHCYASVEFMEDT----PVMVNNIALFEHANRVGNSLLGEPN-V 370
            LK+RI +VI TQA V+ C A+V+F+ED     P  VNN  L      V   +LG  N V
Sbjct: 303 QLKQRIVQVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYV 362

Query: 371 QLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGAALHAA 430
           + LP  M +EDF F+ Q I      +G++N++       HSP F ++EE LP GA+L A+
Sbjct: 363 ETLP-VMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLAS 422

Query: 431 VAMSYLEHNS 435
           +A  YL  +S
Sbjct: 423 LATRYLLDSS 427

BLAST of Lag0012791 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 370.9 bits (951), Expect = 1.4e-102
Identity = 202/427 (47.31%), Postives = 281/427 (65.81%), Query Frame = 0

Query: 15  LVFGVVLWRPS-AAEHGGDGSELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPELGFEE 74
           L F ++L+  S ++E      +   +  KLL  A+  E  +W+  +RR+IHE PELG+EE
Sbjct: 9   LTFQLLLFLLSVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEE 68

Query: 75  YRTSQLVRSELDSLGISYQWPVAKTGVVASIKGASGSSSRPVFALRADMDALPLQELVEW 134
             TS+L+RSEL+ +GI Y++PVA TGV+    G  G+   P  ALRADMDALP+QE VEW
Sbjct: 69  LETSKLIRSELELIGIKYRYPVAITGVI----GYIGTGEPPFVALRADMDALPIQEGVEW 128

Query: 135 EFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEE-CNGAYQMLQE 194
           E KSK+ GKMHACGHD HV MLLGAA++L   R  L+GTV L+FQP EE  +GA +M +E
Sbjct: 129 EHKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREE 188

Query: 195 DALDDIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAATPHKTKDPVLA 254
            AL +++ IF +H+   +P G  ASR G   AGAG F A+I GKGGHAA P  T DPV+A
Sbjct: 189 GALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVA 248

Query: 255 TAFIIQALQQIVSRETDPLEAGVVTVAFVEGGQAENVVPETVKVGGTFRSLSPEGLSYLK 314
            + I+ +LQQ+VSRETDPL++ VVTV+ V GG A NV+P+++ +GGT R+ +  G + L+
Sbjct: 249 ASSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQ 308

Query: 315 ERIREVINTQAVVHHCYASVEF----MEDTPVMVNNIALFEHANRVGNSLLG-EPNVQLL 374
           +R++EVI  QA VH C ASV       E  P  VNN  L++   +V   LLG E  V+  
Sbjct: 309 QRVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAA 368

Query: 375 PWTMGAEDFGFFSQRIAATIYGIGIRNETLRSNRPLHSPHFVLDEEALPIGAALHAAVAM 434
           P  MG+EDF +F++ I      +G+++ET       HSP + ++E+ LP GAA+HA++A+
Sbjct: 369 P-VMGSEDFSYFAETIPGHFSLLGMQDET-NGYASSHSPLYRINEDVLPYGAAIHASMAV 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022925994.17.8e-24195.21IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata] >KAG6604330.1 IAA-amin... [more]
XP_023543889.18.6e-24094.95IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo][more]
XP_022979169.14.3e-23994.98IAA-amino acid hydrolase ILR1-like 3 [Cucurbita maxima][more]
XP_022962856.13.4e-23694.05IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata][more]
XP_023517717.12.9e-23592.15IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P549681.7e-14258.16IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2[more]
Q851L51.2e-13559.31IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8H3C92.1e-13256.83IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q851L63.6e-13257.56IAA-amino acid hydrolase ILR1-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8H3C84.4e-13056.68IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1EDS03.8e-24195.21IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC1114332... [more]
A0A6J1IVY22.1e-23994.98IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111478889... [more]
A0A6J1HEG51.6e-23694.05IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC1114632... [more]
A0A6J1BV661.0e-22591.18IAA-amino acid hydrolase ILR1-like 3 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A0A0KIR81.7e-22589.27M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454350 P... [more]
Match NameE-valueIdentityDescription
AT3G02875.11.2e-14358.16Peptidase M20/M25/M40 family protein [more]
AT1G51760.12.7e-10649.75peptidase M20/M25/M40 family protein [more]
AT1G44350.12.5e-10451.52IAA-leucine resistant (ILR)-like gene 6 [more]
AT1G51780.15.6e-10447.44IAA-leucine resistant (ILR)-like gene 5 [more]
AT5G56660.11.4e-10247.31IAA-leucine resistant (ILR)-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.360coord: 224..338
e-value: 1.4E-131
score: 440.6
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 59..415
e-value: 1.4E-131
score: 440.6
NoneNo IPR availablePANTHERPTHR11014:SF108IAA-AMINO ACID HYDROLASE ILR1coord: 6..433
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 51..432
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 45..434
e-value: 1.5E-117
score: 390.8
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 58..418
e-value: 4.5E-114
score: 379.3
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 6..433
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 117..426
e-value: 3.6E-31
score: 109.0
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 233..324
e-value: 3.4E-10
score: 39.8
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 56..430
e-value: 0.0
score: 593.527
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 227..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012791.1Lag0012791.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
molecular_function GO:0010179 IAA-Ala conjugate hydrolase activity
molecular_function GO:0016787 hydrolase activity