Lag0012503 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0012503 vs. NCBI nr
Match: XP_022978941.1 (cation/H(+) antiporter 20-like [Cucurbita maxima])

HSP 1 Score: 721.5 bits (1861), Expect = 5.1e-204
Identity = 377/457 (82.49%), Postives = 406/457 (88.84%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST-RTHRKLRDLTA 113
           L VL  G+   VLNDE+FAILVLMALFTTFITTPTVMAVYKPARGGST RTHRKL DL+A
Sbjct: 392 LIVLNIGKEKKVLNDEIFAILVLMALFTTFITTPTVMAVYKPARGGSTSRTHRKLHDLSA 451

Query: 114 DDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQ 173
                DDELRILACLHSS NVPSL+ LTE+TR+TKNSSLKLFVMHLVELT+RSSSIMMVQ
Sbjct: 452 -----DDELRILACLHSSGNVPSLMGLTEATRSTKNSSLKLFVMHLVELTERSSSIMMVQ 511

Query: 174 RARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 233
           RARKNGFPFFARFRK GEW DQMA AFQAYSQLGRVKVRPTTAVSSLATMHEDICHVA++
Sbjct: 512 RARKNGFPFFARFRKAGEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVANE 571

Query: 234 KRVTMIILPFHRNWRMVGG----EEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGA 293
           KRVTMIILPFHRNWR+  G    EEENVGHGWRVVNQRVLK+APCSVAVLVDRGFGAN  
Sbjct: 572 KRVTMIILPFHRNWRVFDGDGKEEEENVGHGWRVVNQRVLKNAPCSVAVLVDRGFGANTV 631

Query: 294 QTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSN 353
            T GPG +V V QR+C+VFFGGPDDREALELGG MAEHPAVK TVVRFRPSPS+G EGSN
Sbjct: 632 HTFGPGPIVGVAQRVCIVFFGGPDDREALELGGLMAEHPAVKVTVVRFRPSPSNGFEGSN 691

Query: 354 VILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDATVEYTEREANNMNMMV 413
           VILRP+HSKSGDNHYSFSTAPINRE+EKELDDVA+ EFRSKWDATVE+TE+EA+N NM+V
Sbjct: 692 VILRPMHSKSGDNHYSFSTAPINREKEKELDDVALTEFRSKWDATVEFTEKEASNTNMIV 751

Query: 414 EGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILV 473
           EGVV+I KEGGYDL+VVGKGRVPSSMV KLADR AEHAELGPVGDILASSGRGIVSSILV
Sbjct: 752 EGVVAIGKEGGYDLVVVGKGRVPSSMVVKLADRPAEHAELGPVGDILASSGRGIVSSILV 811

Query: 474 IQQHERGAHSEETPIL-------NKQPLSIDGASTHV 498
           IQQH  G H+EE P+L       N+QPL+ DGAST+V
Sbjct: 812 IQQHGGGGHAEEAPVLKIAESSKNEQPLATDGASTNV 843

BLAST of Lag0012503 vs. NCBI nr
Match: KAG7033846.1 (Cation/H(+) antiporter 20, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 719.9 bits (1857), Expect = 1.5e-203
Identity = 377/457 (82.49%), Postives = 403/457 (88.18%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST-RTHRKLRDLTA 113
           L VL  G+   VLNDE+FAILVLMALFTTFITTPTVMAVYKPARGGST RTHRKL DL  
Sbjct: 392 LIVLNIGKEKKVLNDEIFAILVLMALFTTFITTPTVMAVYKPARGGSTPRTHRKLHDL-- 451

Query: 114 DDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQ 173
              + DDELRILACLHSS NVPSL+ LTE+TR+TKNSSLKLFVMHLVELT+RSSSIMMVQ
Sbjct: 452 ---SGDDELRILACLHSSGNVPSLMGLTEATRSTKNSSLKLFVMHLVELTERSSSIMMVQ 511

Query: 174 RARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 233
           RARKNGFPFFARFRK GEW DQMA AFQAYSQLGRVKVRPTTAVSSLATMHEDICHVA++
Sbjct: 512 RARKNGFPFFARFRKAGEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVANE 571

Query: 234 KRVTMIILPFHRNWRMVGG----EEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGA 293
           KRVTMIILPFHRNWR+  G    EEENVGHGWRVVNQRVLK+APCSVAVLVDRGFGAN  
Sbjct: 572 KRVTMIILPFHRNWRVFDGDGKEEEENVGHGWRVVNQRVLKNAPCSVAVLVDRGFGANTV 631

Query: 294 QTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSN 353
            TPGPG +V V QRIC+VFFGGPDDREALELGG MAEHPAVK TVVRFRPSPS+G EGSN
Sbjct: 632 HTPGPGLMVGVAQRICIVFFGGPDDREALELGGLMAEHPAVKVTVVRFRPSPSNGFEGSN 691

Query: 354 VILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDATVEYTEREANNMNMMV 413
           VILRP+HSKSGDN YSFSTAPIN E+EKELDDVA+ EFRSKWDATVEYTE+EANNMNM+V
Sbjct: 692 VILRPIHSKSGDNRYSFSTAPINGEKEKELDDVALTEFRSKWDATVEYTEKEANNMNMIV 751

Query: 414 EGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILV 473
           EGVV I KEGGYDL+VVGKGRVPSSMV KLADR AEHAELGPVGDILASSGRGIVSSILV
Sbjct: 752 EGVVGIGKEGGYDLVVVGKGRVPSSMVVKLADRPAEHAELGPVGDILASSGRGIVSSILV 811

Query: 474 IQQHERGAHSEETPIL-------NKQPLSIDGASTHV 498
           IQQH  G H+EE P+L       N+QPL+ DG S++V
Sbjct: 812 IQQHGGGGHAEEAPVLKIAESSKNEQPLATDGPSSNV 843

BLAST of Lag0012503 vs. NCBI nr
Match: XP_038883335.1 (cation/H(+) antiporter 20 isoform X2 [Benincasa hispida])

HSP 1 Score: 718.4 bits (1853), Expect = 4.3e-203
Identity = 376/458 (82.10%), Postives = 406/458 (88.65%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST-RTHRKLRDLTA 113
           L VL  G+   VLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST  THRKLRDL+A
Sbjct: 393 LIVLNIGKEKKVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTPPTHRKLRDLSA 452

Query: 114 DDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQ 173
           DD+  DDELRILAC+HSS NVPSLI+LTESTR+TKNSSLKLFVMHLVELT+RSSSIMMVQ
Sbjct: 453 DDT-IDDELRILACVHSSGNVPSLITLTESTRSTKNSSLKLFVMHLVELTERSSSIMMVQ 512

Query: 174 RARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 233
           RARKNGFPFFARFRK  EW DQMA AFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD
Sbjct: 513 RARKNGFPFFARFRKASEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 572

Query: 234 KRVTMIILPFHRNWRMVGG-------EEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGA 293
           KRVTMIILPFHRNWR  GG        EENVGHGWRVVNQRVLK++PCSVAVLVDRGFGA
Sbjct: 573 KRVTMIILPFHRNWRAFGGGDVAEEEVEENVGHGWRVVNQRVLKNSPCSVAVLVDRGFGA 632

Query: 294 NGAQTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVE 353
             AQTPGPG +V VG+RICV+FFGGPDDREALELGGRMAEHP VK TVVRFRPS +DG+E
Sbjct: 633 AAAQTPGPGPMVGVGRRICVLFFGGPDDREALELGGRMAEHPVVKVTVVRFRPSSADGME 692

Query: 354 GSNVILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDATVEYTEREANNMN 413
           GSNVILRP+HSKSGDNHYSF+T PINRE+EKELDD A+AEFRSKW+ATVEY E+E +NMN
Sbjct: 693 GSNVILRPMHSKSGDNHYSFTTTPINREKEKELDDAALAEFRSKWEATVEYKEKEVSNMN 752

Query: 414 MMVEGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSS 473
           M+VEGV+++ KE GYDLIVVGKGRVPSSMVAKLADR  EHAELGPVGD+LASSG+GIVSS
Sbjct: 753 MIVEGVMALGKECGYDLIVVGKGRVPSSMVAKLADRPVEHAELGPVGDLLASSGKGIVSS 812

Query: 474 ILVIQQHERGAHSEETPIL-------NKQPLSIDGAST 496
           IL+IQQH  GAH EETP+L       N+ PLS DGAST
Sbjct: 813 ILIIQQHGGGAHVEETPVLKIAQSNKNELPLSTDGAST 849

BLAST of Lag0012503 vs. NCBI nr
Match: XP_038883334.1 (cation/H(+) antiporter 20 isoform X1 [Benincasa hispida])

HSP 1 Score: 718.4 bits (1853), Expect = 4.3e-203
Identity = 376/458 (82.10%), Postives = 406/458 (88.65%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST-RTHRKLRDLTA 113
           L VL  G+   VLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST  THRKLRDL+A
Sbjct: 394 LIVLNIGKEKKVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTPPTHRKLRDLSA 453

Query: 114 DDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQ 173
           DD+  DDELRILAC+HSS NVPSLI+LTESTR+TKNSSLKLFVMHLVELT+RSSSIMMVQ
Sbjct: 454 DDT-IDDELRILACVHSSGNVPSLITLTESTRSTKNSSLKLFVMHLVELTERSSSIMMVQ 513

Query: 174 RARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 233
           RARKNGFPFFARFRK  EW DQMA AFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD
Sbjct: 514 RARKNGFPFFARFRKASEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 573

Query: 234 KRVTMIILPFHRNWRMVGG-------EEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGA 293
           KRVTMIILPFHRNWR  GG        EENVGHGWRVVNQRVLK++PCSVAVLVDRGFGA
Sbjct: 574 KRVTMIILPFHRNWRAFGGGDVAEEEVEENVGHGWRVVNQRVLKNSPCSVAVLVDRGFGA 633

Query: 294 NGAQTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVE 353
             AQTPGPG +V VG+RICV+FFGGPDDREALELGGRMAEHP VK TVVRFRPS +DG+E
Sbjct: 634 AAAQTPGPGPMVGVGRRICVLFFGGPDDREALELGGRMAEHPVVKVTVVRFRPSSADGME 693

Query: 354 GSNVILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDATVEYTEREANNMN 413
           GSNVILRP+HSKSGDNHYSF+T PINRE+EKELDD A+AEFRSKW+ATVEY E+E +NMN
Sbjct: 694 GSNVILRPMHSKSGDNHYSFTTTPINREKEKELDDAALAEFRSKWEATVEYKEKEVSNMN 753

Query: 414 MMVEGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSS 473
           M+VEGV+++ KE GYDLIVVGKGRVPSSMVAKLADR  EHAELGPVGD+LASSG+GIVSS
Sbjct: 754 MIVEGVMALGKECGYDLIVVGKGRVPSSMVAKLADRPVEHAELGPVGDLLASSGKGIVSS 813

Query: 474 ILVIQQHERGAHSEETPIL-------NKQPLSIDGAST 496
           IL+IQQH  GAH EETP+L       N+ PLS DGAST
Sbjct: 814 ILIIQQHGGGAHVEETPVLKIAQSNKNELPLSTDGAST 850

BLAST of Lag0012503 vs. NCBI nr
Match: KAG6603667.1 (Cation/H(+) antiporter 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 716.1 bits (1847), Expect = 2.1e-202
Identity = 376/457 (82.28%), Postives = 401/457 (87.75%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST-RTHRKLRDLTA 113
           L VL  G+   VLNDE+FAILVLMALFTTFITTPTVMAVYKPARGGST RTHRKL DL  
Sbjct: 142 LIVLNIGKEKKVLNDEIFAILVLMALFTTFITTPTVMAVYKPARGGSTPRTHRKLHDL-- 201

Query: 114 DDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQ 173
              + DDELRILACLHSS NV SL+ LTE+TR+TKNSSLKLFVMHLVELT+RSSSIMMVQ
Sbjct: 202 ---SGDDELRILACLHSSGNVQSLMGLTEATRSTKNSSLKLFVMHLVELTERSSSIMMVQ 261

Query: 174 RARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 233
           RARKNGFPFFARFRK GEW DQMA AFQAYSQLGRVKVRPTTAVSSLATMHEDICHVA++
Sbjct: 262 RARKNGFPFFARFRKAGEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVANE 321

Query: 234 KRVTMIILPFHRNWRMVGG----EEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGA 293
           KRVTMIILPFHRNWR+  G    EEENVGHGWRVVNQRVLK+APCSVAVLVDRGFGAN  
Sbjct: 322 KRVTMIILPFHRNWRVFDGDGKEEEENVGHGWRVVNQRVLKNAPCSVAVLVDRGFGANTV 381

Query: 294 QTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSN 353
            TPGPG +V V QRIC+VFFGGPDDREALELGG MAEHPAVK TVVRFRPSPS+G EGSN
Sbjct: 382 HTPGPGLMVGVAQRICIVFFGGPDDREALELGGLMAEHPAVKVTVVRFRPSPSNGFEGSN 441

Query: 354 VILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDATVEYTEREANNMNMMV 413
           VILRP+HSKSGDN YSFSTAPIN E+EKELDDVA+ EFRSKWDATVEYTE+EANN NM+V
Sbjct: 442 VILRPIHSKSGDNRYSFSTAPINGEKEKELDDVALTEFRSKWDATVEYTEKEANNTNMIV 501

Query: 414 EGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILV 473
           EGVV I KEGGYDL+VVGKGRVPSSMV KLADR AEHAELGPVGDILASSGRGIVSSILV
Sbjct: 502 EGVVGIGKEGGYDLVVVGKGRVPSSMVVKLADRPAEHAELGPVGDILASSGRGIVSSILV 561

Query: 474 IQQHERGAHSEETPIL-------NKQPLSIDGASTHV 498
           IQQH  G H+EE P+L       N+QPL+ DG ST+V
Sbjct: 562 IQQHGGGGHAEEAPVLKIAESSKNEQPLATDGPSTNV 593

BLAST of Lag0012503 vs. ExPASy Swiss-Prot
Match: Q9M353 (Cation/H(+) antiporter 20 OS=Arabidopsis thaliana OX=3702 GN=CHX20 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 6.6e-138
Identity = 279/484 (57.64%), Postives = 340/484 (70.25%), Query Frame = 0

Query: 32  FSATAFTKLSIAGVLTVGLCSG------LFVLAAGQNLLVLNDEVFAILVLMALFTTFIT 91
           F      K+     LT+G          L VL  G+   VLNDE FAILVLMALFTTFIT
Sbjct: 367 FVVAVMVKVPAREALTLGFLMNTKGLVELIVLNIGKEKKVLNDETFAILVLMALFTTFIT 426

Query: 92  TPTVMAVYKPARGGSTRTHRKLRDLTADDSNSDDELRILACLHSSDNVPSLISLTESTRN 151
           TPTVMA+YKPARG    THRKL+DL+A   ++ +ELRILACLH   NV SLISL ES R 
Sbjct: 427 TPTVMAIYKPARG----THRKLKDLSASQDSTKEELRILACLHGPANVSSLISLVESIRT 486

Query: 152 TKNSSLKLFVMHLVELTKRSSSIMMVQRARKNGFPFFARFRKGEWHDQMAGAFQAYSQLG 211
           TK   LKLFVMHL+ELT+RSSSI+MVQRARKNG PF  R+R GE H  + G F+AY QLG
Sbjct: 487 TKILRLKLFVMHLMELTERSSSIIMVQRARKNGLPFVHRYRHGERHSNVIGGFEAYRQLG 546

Query: 212 RVKVRPTTAVSSLATMHEDICHVADDKRVTMIILPFHRNWRMVGGEE------------- 271
           RV VRP TAVS L TMHEDICH+AD KRVTMIILPFH+ W    G               
Sbjct: 547 RVAVRPITAVSPLPTMHEDICHMADTKRVTMIILPFHKRWNADHGHSHHHQDGGGDGNVP 606

Query: 272 ENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQTPG-PGSLVAVGQRICVVFFGGPD 331
           ENVGHGWR+VNQRVLK+APCSVAVLVDRG G+  AQT    GS V   +R+CV+FFGGPD
Sbjct: 607 ENVGHGWRLVNQRVLKNAPCSVAVLVDRGLGSIEAQTLSLDGSNVV--ERVCVIFFGGPD 666

Query: 332 DREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNVILRPLHSKSGDNHYSFSTAPINR 391
           DRE++ELGGRMAEHPAVK TV+RF     + +  + V LRP  SK  + +Y+F T  ++ 
Sbjct: 667 DRESIELGGRMAEHPAVKVTVIRF--LVRETLRSTAVTLRPAPSKGKEKNYAFLTTNVDP 726

Query: 392 EREKELDDVAIAEFRSKWDATVEYTEREANNMNMMVEGVVSIRKEGGYDLIVVGKGRVPS 451
           E+EKELD+ A+ +F+SKW   VEY E+E NN   ++E ++SI +   +DLIVVG+GR+PS
Sbjct: 727 EKEKELDEGALEDFKSKWKEMVEYKEKEPNN---IIEEILSIGQSKDFDLIVVGRGRIPS 786

Query: 452 SMVAKLADRAAEHAELGPVGDILASSGRGIVSSILVIQQHERGAHSEE---TPILNKQPL 493
           + VA LA+R AEH ELGP+GD+LASS   I+ SILV+QQH + AH E+   + I+++  L
Sbjct: 787 AEVAALAERQAEHPELGPIGDVLASSINHIIPSILVVQQHNK-AHVEDITVSKIVSESSL 838

BLAST of Lag0012503 vs. ExPASy Swiss-Prot
Match: Q9LUN4 (Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 7.9e-75
Identity = 180/427 (42.15%), Postives = 254/427 (59.48%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTRTHRKLRDLTAD 113
           L VL  G++  VLND+ FAILVLMALFTTFITTP VM +YKPAR G+   HR     T  
Sbjct: 390 LIVLNIGKDRKVLNDQAFAILVLMALFTTFITTPIVMLIYKPARKGAPYKHR-----TIQ 449

Query: 114 DSNSDDELRILACLHSSDNVPSLISLTESTRNT-KNSSLKLFVMHLVELTKRSSSIMMVQ 173
             + D ELRILAC HS+ N+P+LI+L ES+R T K   L ++ MHL+EL++RSS+I MV 
Sbjct: 450 RKDHDSELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVH 509

Query: 174 RARKNGFPFFARFRKGEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADDK 233
           +AR NG P + +  +    DQM  AF+AY  L  V VRP TA+S L+++HEDIC  A  K
Sbjct: 510 KARNNGLPIWNKIERST--DQMVIAFEAYQHLRAVAVRPMTAISGLSSIHEDICTSAHQK 569

Query: 234 RVTMIILPFHRNWRMVGGEEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQTPGP 293
           RV MI+LPFH++ RM  G  E++GH +  VNQRVL+ APCSV +LVDRG G       G 
Sbjct: 570 RVAMILLPFHKHQRM-DGAMESIGHRFHEVNQRVLQRAPCSVGILVDRGLG-------GT 629

Query: 294 GSLVA--VGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNVIL 353
             +VA  V  ++ + FFGG DDREAL  G +M EHP +  TV +F               
Sbjct: 630 SQVVASEVAYKVVIPFFGGLDDREALAYGMKMVEHPGITLTVYKF--------------- 689

Query: 354 RPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRS--KWDATVEYTEREANNMNMMVE 413
             + ++     +  S      ++EKE D+  + E  +  + + ++ Y ER   + + ++ 
Sbjct: 690 --VAARGTLKRFEKSEHDEKEKKEKETDEEFVRELMNDPRGNESLAYEERVVESKDDIIA 749

Query: 414 GVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILVI 473
            + S+ K    +L VVG+    +S+V     ++ +  ELGPVG +L+SS     +S+LV+
Sbjct: 750 TLKSMSK---CNLFVVGRNAAVASLV-----KSTDCPELGPVGRLLSSSEFSTTASVLVV 776

Query: 474 QQHERGA 476
           Q ++  A
Sbjct: 810 QGYDPAA 776

BLAST of Lag0012503 vs. ExPASy Swiss-Prot
Match: Q9FFR9 (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 9.7e-73
Identity = 177/421 (42.04%), Postives = 247/421 (58.67%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTRTHRKLRDLTAD 113
           L VL  G++  VLND+ FAI+VLMALFTTFITTP VMAVYKPAR        K R +  +
Sbjct: 389 LIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEGEYKHRAVERE 448

Query: 114 DSNSDDELRILACLHSSDNVPSLISLTESTRN-TKNSSLKLFVMHLVELTKRSSSIMMVQ 173
           ++N+  +LRIL C H + ++PS+I+L E++R   K   L ++ +HL EL++RSS+I+MV 
Sbjct: 449 NTNT--QLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSERSSAILMVH 508

Query: 174 RARKNGFPFFARFRKGEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADDK 233
           + RKNG PF+ R       DQ+  AFQA+ QL RV VRP TA+SS++ +HEDIC  A  K
Sbjct: 509 KVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSRVNVRPMTAISSMSDIHEDICTTAVRK 568

Query: 234 RVTMIILPFHRNWRMVGGEEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQTPGP 293
           +  ++ILPFH++ ++ G  E   G  +R VN+RVL  APCSV + VDRG G +       
Sbjct: 569 KAAIVILPFHKHQQLDGSLETTRG-DYRWVNRRVLLQAPCSVGIFVDRGLGGSSQV---- 628

Query: 294 GSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNVILRP 353
            S   V   + V+FFGGPDDREAL  G RMAEHP +  TV RF  SP    E  NV    
Sbjct: 629 -SAQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPERVGEIVNV---- 688

Query: 354 LHSKSGDNHYSFSTAPINREREKELDDVAIAEFR--SKWDATVEYTEREANNMNMMVEGV 413
               S +N+ + S       +  + D+  ++E R  S  D +V++ E++  N  + V   
Sbjct: 689 --EVSNNNNENQSV------KNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSA 748

Query: 414 VSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILVIQQ 472
           +   +     L+    GR+P   +A      +E  ELGPVG +L S      +S+LVIQQ
Sbjct: 749 IEEVRRSNLFLV----GRMPGGEIALAIRENSECPELGPVGSLLISPESSTKASVLVIQQ 785

BLAST of Lag0012503 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.3e-64
Identity = 164/445 (36.85%), Postives = 246/445 (55.28%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTRTHRKLRDLTAD 113
           + VL  G++  VL+DE FA +VL+AL  T + TP V  +YKP +   +   R     T  
Sbjct: 393 MIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIVTILYKPVKKSVSYKRR-----TIQ 452

Query: 114 DSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQR 173
            +  D ELR+L C+H+  NVP++I+L E++  TK S + ++V+HLVELT R+S++++V  
Sbjct: 453 QTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHN 512

Query: 174 ARKNGFPFFARFRKGEWHDQMAGAFQAYSQ-LGRVKVRPTTAVSSLATMHEDICHVADDK 233
            RK+G P  A  R     D +  AF+ Y Q    V V+P TA+S  +TMHED+C +A+DK
Sbjct: 513 TRKSGRP--ALNRTQAQSDHIINAFENYEQHAAFVAVQPLTAISPYSTMHEDVCSLAEDK 572

Query: 234 RVTMIILPFHRNWRMVGGEEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQTPGP 293
           RV+ II+PFH+  + V G  E+    +R+VNQ +L+++PCSV +LVDRG   NGA     
Sbjct: 573 RVSFIIIPFHKQ-QTVDGGMESTNPAYRLVNQNLLENSPCSVGILVDRGL--NGATRLNS 632

Query: 294 GSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNVILRP 353
            +   V  ++ V+FFGGPDDREAL    RMA+HP +  TV+RF     +    S      
Sbjct: 633 NT---VSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDEDEADTAST----- 692

Query: 354 LHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSK--WDATVEYTEREANNMNMMVEGV 413
                  N        ++  ++++LDD  I  FR++     ++ Y E+  +N    V  V
Sbjct: 693 ----RATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVAAV 752

Query: 414 VSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILVIQQ 473
            S+  +  +DL +VG+G   SS +       +E  ELG +GD+LASS      S+LV+QQ
Sbjct: 753 RSM--DSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQ 812

Query: 474 H-------------ERGAHSEETPI 483
           +             E   HS ET +
Sbjct: 813 YVGSWAQEDDMDFPESPVHSHETKV 813

BLAST of Lag0012503 vs. ExPASy Swiss-Prot
Match: Q9SUQ7 (Cation/H(+) antiporter 17 OS=Arabidopsis thaliana OX=3702 GN=CHX17 PE=1 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.4e-63
Identity = 157/424 (37.03%), Postives = 246/424 (58.02%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTRTHRKLRDLTAD 113
           L VL  G++  VLND++FAI+VLMA+FTTF+TTP V+AVYKP   G + T    ++ T +
Sbjct: 388 LIVLNIGKDRGVLNDQIFAIMVLMAIFTTFMTTPLVLAVYKP---GKSLTKADYKNRTVE 447

Query: 114 DSN-SDDELRILACLHSSDNVPSLISLTESTRN-TKNSSLKLFVMHLVELTKRSSSIMMV 173
           ++N S+  L ++ C  S  N+P++++L E++R   +  +L ++ MHL+EL++RSS+I+M 
Sbjct: 448 ETNRSNKPLCLMFCFQSIMNIPTIVNLIEASRGINRKENLSVYAMHLMELSERSSAILMA 507

Query: 174 QRARKNGFPFFARFR---KGEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHV 233
            + R+NG PF+ + +        D +  AF+A+ +L RV VRP TA+S +AT+HEDIC  
Sbjct: 508 HKVRRNGLPFWNKDKSENNSSSSDMVVVAFEAFRRLSRVSVRPMTAISPMATIHEDICQS 567

Query: 234 ADDKRVTMIILPFHRNWRMVGGEEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQ 293
           A+ K+  M+ILPFH++ R+     E   + +R +N++V++ +PCSVA+LVDRG G     
Sbjct: 568 AERKKTAMVILPFHKHVRL-DRTWETTRNDYRWINKKVMEESPCSVAILVDRGLGGTTRV 627

Query: 294 TPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNV 353
                SL      I V+FFGG DDREAL    RMAEHP +  TVVRF   PSD  +  NV
Sbjct: 628 ASSDFSLT-----ITVLFFGGNDDREALAFAVRMAEHPGISLTVVRF--IPSDEFKPENV 687

Query: 354 ILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDATVEYTEREANNMNMMVE 413
            +     +               E + ++ +   +   S  ++ + Y E+       ++E
Sbjct: 688 RIEITEDQLCSGATRLIDIEAITELKAKIKEKESSRSNSDSESHIIYEEKIVKCYEEVIE 747

Query: 414 GVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILA-SSGRGIVSSILV 472
            +    K    +L +VGK   P   VA   +  ++  ELGP+G++L  S     V+S+LV
Sbjct: 748 VIKEYSKS---NLFLVGKS--PEGSVASGINVRSDTPELGPIGNLLTESESVSTVASVLV 795

BLAST of Lag0012503 vs. ExPASy TrEMBL
Match: A0A6J1IPD5 (cation/H(+) antiporter 20-like OS=Cucurbita maxima OX=3661 GN=LOC111478742 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 2.5e-204
Identity = 377/457 (82.49%), Postives = 406/457 (88.84%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST-RTHRKLRDLTA 113
           L VL  G+   VLNDE+FAILVLMALFTTFITTPTVMAVYKPARGGST RTHRKL DL+A
Sbjct: 392 LIVLNIGKEKKVLNDEIFAILVLMALFTTFITTPTVMAVYKPARGGSTSRTHRKLHDLSA 451

Query: 114 DDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQ 173
                DDELRILACLHSS NVPSL+ LTE+TR+TKNSSLKLFVMHLVELT+RSSSIMMVQ
Sbjct: 452 -----DDELRILACLHSSGNVPSLMGLTEATRSTKNSSLKLFVMHLVELTERSSSIMMVQ 511

Query: 174 RARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 233
           RARKNGFPFFARFRK GEW DQMA AFQAYSQLGRVKVRPTTAVSSLATMHEDICHVA++
Sbjct: 512 RARKNGFPFFARFRKAGEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVANE 571

Query: 234 KRVTMIILPFHRNWRMVGG----EEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGA 293
           KRVTMIILPFHRNWR+  G    EEENVGHGWRVVNQRVLK+APCSVAVLVDRGFGAN  
Sbjct: 572 KRVTMIILPFHRNWRVFDGDGKEEEENVGHGWRVVNQRVLKNAPCSVAVLVDRGFGANTV 631

Query: 294 QTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSN 353
            T GPG +V V QR+C+VFFGGPDDREALELGG MAEHPAVK TVVRFRPSPS+G EGSN
Sbjct: 632 HTFGPGPIVGVAQRVCIVFFGGPDDREALELGGLMAEHPAVKVTVVRFRPSPSNGFEGSN 691

Query: 354 VILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDATVEYTEREANNMNMMV 413
           VILRP+HSKSGDNHYSFSTAPINRE+EKELDDVA+ EFRSKWDATVE+TE+EA+N NM+V
Sbjct: 692 VILRPMHSKSGDNHYSFSTAPINREKEKELDDVALTEFRSKWDATVEFTEKEASNTNMIV 751

Query: 414 EGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILV 473
           EGVV+I KEGGYDL+VVGKGRVPSSMV KLADR AEHAELGPVGDILASSGRGIVSSILV
Sbjct: 752 EGVVAIGKEGGYDLVVVGKGRVPSSMVVKLADRPAEHAELGPVGDILASSGRGIVSSILV 811

Query: 474 IQQHERGAHSEETPIL-------NKQPLSIDGASTHV 498
           IQQH  G H+EE P+L       N+QPL+ DGAST+V
Sbjct: 812 IQQHGGGGHAEEAPVLKIAESSKNEQPLATDGASTNV 843

BLAST of Lag0012503 vs. ExPASy TrEMBL
Match: A0A6J1GDC1 (cation/H(+) antiporter 20-like OS=Cucurbita moschata OX=3662 GN=LOC111453157 PE=4 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 2.5e-201
Identity = 374/457 (81.84%), Postives = 401/457 (87.75%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST-RTHRKLRDLTA 113
           L VL  G+   VLNDE+FAILVLMALFTTFITTPTVMAVYKPARGGST RTHRKL DL  
Sbjct: 392 LIVLNIGKEKKVLNDEIFAILVLMALFTTFITTPTVMAVYKPARGGSTPRTHRKLHDL-- 451

Query: 114 DDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQ 173
              + DDELRILACLHSS NVPSL+ LTE+TR+TKNSSLKLFVMHLVELT+RSSSIMMVQ
Sbjct: 452 ---SGDDELRILACLHSSGNVPSLMGLTEATRSTKNSSLKLFVMHLVELTERSSSIMMVQ 511

Query: 174 RARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 233
           RARKNGFPFFARFRK GEW DQMA AFQAYSQLGRVKVRPTTAVSSLATMHEDICHVA++
Sbjct: 512 RARKNGFPFFARFRKAGEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVANE 571

Query: 234 KRVTMIILPFHRNWRMVGG----EEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGA 293
           KRVTMIILPFHRNWR+  G    EEENVGHGWRVVNQRVLK+APCSVAVLVDRGFGAN  
Sbjct: 572 KRVTMIILPFHRNWRVFDGDGKEEEENVGHGWRVVNQRVLKNAPCSVAVLVDRGFGANTV 631

Query: 294 QTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSN 353
            TPGPG +V V QRIC+VFFGGPDDREALELGG MAEHPAVK TVVRFRPSPS+G EGSN
Sbjct: 632 HTPGPGLMVGVAQRICIVFFGGPDDREALELGGLMAEHPAVKVTVVRFRPSPSNGFEGSN 691

Query: 354 VILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDATVEYTEREANNMNMMV 413
            ILRP+HSKSGDN YSFST PINRE+EKELD+VA+ EFRSKWDATVEYTE+EA+N NM+V
Sbjct: 692 FILRPMHSKSGDNRYSFSTDPINREKEKELDNVALTEFRSKWDATVEYTEKEASNTNMIV 751

Query: 414 EGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILV 473
           EGVV I KEGGYDL+VVGKGRVPSSMV KLADR AEHAELGPVGDILASS RGIVSSILV
Sbjct: 752 EGVVGIGKEGGYDLVVVGKGRVPSSMVVKLADRPAEHAELGPVGDILASSSRGIVSSILV 811

Query: 474 IQQHERGAHSEETPIL-------NKQPLSIDGASTHV 498
           IQQH  G H+EE  +L       N+QPL+IDGAST+V
Sbjct: 812 IQQHGGGGHAEEALVLKIAESSKNEQPLAIDGASTNV 843

BLAST of Lag0012503 vs. ExPASy TrEMBL
Match: A0A1S3BIV3 (cation/H(+) antiporter 20 OS=Cucumis melo OX=3656 GN=LOC103490056 PE=4 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.3e-197
Identity = 373/471 (79.19%), Postives = 404/471 (85.77%), Query Frame = 0

Query: 44  GVL--TVGLCSGLFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST 103
           GVL  T GL   L VL  G+   VLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST
Sbjct: 384 GVLMNTKGLVE-LIVLNIGKEKKVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST 443

Query: 104 -RTHRKLRDLTADDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVE 163
             THRKLRDL+A+DS  +DELRILAC+HSS NVPSLI+LTESTR+TKNSSLKLFVMHLVE
Sbjct: 444 PPTHRKLRDLSANDSPVEDELRILACVHSSGNVPSLITLTESTRSTKNSSLKLFVMHLVE 503

Query: 164 LTKRSSSIMMVQRARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLA 223
           LT+RSSSIMMVQRARKNGFPFFARFRK  EW DQMA AFQAYSQLGRVKVRPTTAVSSLA
Sbjct: 504 LTERSSSIMMVQRARKNGFPFFARFRKASEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLA 563

Query: 224 TMHEDICHVADDKRVTMIILPFHRNWRMVGG-------EEENVGHGWRVVNQRVLKSAPC 283
           TMHEDICHVADDKRVTMIILPFHRNWR  GG        EENVGHGWRVVNQRVLK+APC
Sbjct: 564 TMHEDICHVADDKRVTMIILPFHRNWRAFGGGDGAEEEVEENVGHGWRVVNQRVLKNAPC 623

Query: 284 SVAVLVDRGFGANGAQTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTV 343
           SVAVLVDRGFGA  AQTPGPG ++ VGQRICV+FFGGPDDREALELGGRMAEHPAVK TV
Sbjct: 624 SVAVLVDRGFGAGVAQTPGPGPMICVGQRICVLFFGGPDDREALELGGRMAEHPAVKVTV 683

Query: 344 VRFRPSPSDGVEGSNVILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDAT 403
           VRFRPS +DG+EGSNVILRP HSKS DNHYSF T PINRE+EKE D+ A+AEF+SKW+AT
Sbjct: 684 VRFRPSSADGMEGSNVILRPTHSKSSDNHYSFITTPINREKEKEQDETALAEFKSKWEAT 743

Query: 404 VEYTEREANNMNMMVEGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGD 463
           VEY E+E ++ NM+VEGVV++ KE  YDLIVVGKGRVPSS+V KLADR AEHAELGPVGD
Sbjct: 744 VEYKEKEVSSTNMIVEGVVALGKEMSYDLIVVGKGRVPSSLVMKLADRPAEHAELGPVGD 803

Query: 464 ILASSGRGIVSSILVIQQHERGAHSEETPIL---------NKQPLSIDGAS 495
           ILASSG+GIVSSIL+IQQH  G H EETP+L         N+ P+S DG S
Sbjct: 804 ILASSGKGIVSSILIIQQHGGGGHVEETPVLKIAQSNKNENELPMSNDGTS 853

BLAST of Lag0012503 vs. ExPASy TrEMBL
Match: A0A5D3DIK0 (Cation/H(+) antiporter 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G00030 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 8.5e-197
Identity = 365/459 (79.52%), Postives = 397/459 (86.49%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST-RTHRKLRDLTA 113
           LF + +     VLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST  THRKLRDL+A
Sbjct: 7   LFPIQSLDQTQVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTPPTHRKLRDLSA 66

Query: 114 DDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQ 173
           +DS  +DELRILAC+HSS NVPSLI+LTESTR+TKNSSLKLFVMHLVELT+RSSSIMMVQ
Sbjct: 67  NDSPVEDELRILACVHSSGNVPSLITLTESTRSTKNSSLKLFVMHLVELTERSSSIMMVQ 126

Query: 174 RARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 233
           RARKNGFPFFARFRK  EW DQMA AFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD
Sbjct: 127 RARKNGFPFFARFRKASEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADD 186

Query: 234 KRVTMIILPFHRNWRMVGG-------EEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGA 293
           KRVTMIILPFHRNWR  GG        EENVGHGWRVVNQRVLK+APCSVAVLVDRGFGA
Sbjct: 187 KRVTMIILPFHRNWRAFGGGDGAEEEVEENVGHGWRVVNQRVLKNAPCSVAVLVDRGFGA 246

Query: 294 NGAQTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVE 353
             AQTPGPG ++ VGQRICV+FFGGPDDREALELGGRMAEHPAVK TVVRFRPS +DG+E
Sbjct: 247 GVAQTPGPGPMICVGQRICVLFFGGPDDREALELGGRMAEHPAVKVTVVRFRPSSADGME 306

Query: 354 GSNVILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDATVEYTEREANNMN 413
           GSNVILRP HSKS DNHYSF T PINRE+EKE D+ A+AEF+SKW+ATVEY E+E ++ N
Sbjct: 307 GSNVILRPTHSKSSDNHYSFITTPINREKEKEQDETALAEFKSKWEATVEYKEKEVSSTN 366

Query: 414 MMVEGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSS 473
           M+VEGVV++ KE  YDLIVVGKGRVPSS+V KLADR AEHAELGPVGDILASSG+GIVSS
Sbjct: 367 MIVEGVVALGKEMSYDLIVVGKGRVPSSLVMKLADRPAEHAELGPVGDILASSGKGIVSS 426

Query: 474 ILVIQQHERGAHSEETPIL---------NKQPLSIDGAS 495
           IL+IQQH  G H EETP+L         N+ P+S DG S
Sbjct: 427 ILIIQQHGGGGHVEETPVLKIAQSNKNENELPMSNDGTS 465

BLAST of Lag0012503 vs. ExPASy TrEMBL
Match: A0A0A0KXF6 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G294410 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.6e-195
Identity = 367/469 (78.25%), Postives = 400/469 (85.29%), Query Frame = 0

Query: 44  GVL--TVGLCSGLFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST 103
           GVL  T GL   L VL  G+   VLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST
Sbjct: 384 GVLMNTKGLVE-LIVLNIGKEKKVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGST 443

Query: 104 -RTHRKLRDLTADDSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVE 163
             THRKLRDL+A+DS  +DELRILAC+HSS NVPSLI+LTESTR+T+NSSLKLFVMHLVE
Sbjct: 444 PPTHRKLRDLSANDSPVNDELRILACVHSSGNVPSLITLTESTRSTRNSSLKLFVMHLVE 503

Query: 164 LTKRSSSIMMVQRARKNGFPFFARFRK-GEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLA 223
           LT+RSSSIMMVQRARKNGFPFFARFRK  EW DQMA AFQAYSQLGRVKVRPTTAVSSL 
Sbjct: 504 LTERSSSIMMVQRARKNGFPFFARFRKASEWRDQMAAAFQAYSQLGRVKVRPTTAVSSLT 563

Query: 224 TMHEDICHVADDKRVTMIILPFHRNWRMVGG-------EEENVGHGWRVVNQRVLKSAPC 283
           TMHEDICHVADDKRVTMIILPFHRNWR  GG        EENVGHGWRVVNQRVLK+APC
Sbjct: 564 TMHEDICHVADDKRVTMIILPFHRNWRAFGGGDGAEEEVEENVGHGWRVVNQRVLKNAPC 623

Query: 284 SVAVLVDRGFGANGAQTPGPGSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTV 343
           SVAVLVDRGFGA  AQTPGPGS++ VGQRICV+FFGGPDDREALELGGRMAEHPAVK TV
Sbjct: 624 SVAVLVDRGFGAGVAQTPGPGSMICVGQRICVLFFGGPDDREALELGGRMAEHPAVKVTV 683

Query: 344 VRFRPSPSDGVEGSNVILRPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSKWDAT 403
           VRFRPS  D  EGSNVILRP+HSKS DNHYSF T PINRE+EKE+D+ A+AEF+SKW+AT
Sbjct: 684 VRFRPSSGDVTEGSNVILRPMHSKSNDNHYSFITTPINREKEKEVDEAALAEFKSKWEAT 743

Query: 404 VEYTEREANNMNMMVEGVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGD 463
           VEY E+E ++ NM+VEGVV++ KE  YDLIVVGKGRVPSS+V KLADR AEHAELGPVGD
Sbjct: 744 VEYKEKEVSSTNMIVEGVVALGKEDSYDLIVVGKGRVPSSLVMKLADRPAEHAELGPVGD 803

Query: 464 ILASSGRGIVSSILVIQQHERGAHSEETPIL-------NKQPLSIDGAS 495
           ILASSG+GI SSIL++QQH    H EE P+L       N+ P+S DG S
Sbjct: 804 ILASSGKGITSSILIVQQHGGSGHVEEAPVLKIAQSNKNELPMSTDGTS 851

BLAST of Lag0012503 vs. TAIR 10
Match: AT3G53720.1 (cation/H+ exchanger 20 )

HSP 1 Score: 492.3 bits (1266), Expect = 4.7e-139
Identity = 279/484 (57.64%), Postives = 340/484 (70.25%), Query Frame = 0

Query: 32  FSATAFTKLSIAGVLTVGLCSG------LFVLAAGQNLLVLNDEVFAILVLMALFTTFIT 91
           F      K+     LT+G          L VL  G+   VLNDE FAILVLMALFTTFIT
Sbjct: 367 FVVAVMVKVPAREALTLGFLMNTKGLVELIVLNIGKEKKVLNDETFAILVLMALFTTFIT 426

Query: 92  TPTVMAVYKPARGGSTRTHRKLRDLTADDSNSDDELRILACLHSSDNVPSLISLTESTRN 151
           TPTVMA+YKPARG    THRKL+DL+A   ++ +ELRILACLH   NV SLISL ES R 
Sbjct: 427 TPTVMAIYKPARG----THRKLKDLSASQDSTKEELRILACLHGPANVSSLISLVESIRT 486

Query: 152 TKNSSLKLFVMHLVELTKRSSSIMMVQRARKNGFPFFARFRKGEWHDQMAGAFQAYSQLG 211
           TK   LKLFVMHL+ELT+RSSSI+MVQRARKNG PF  R+R GE H  + G F+AY QLG
Sbjct: 487 TKILRLKLFVMHLMELTERSSSIIMVQRARKNGLPFVHRYRHGERHSNVIGGFEAYRQLG 546

Query: 212 RVKVRPTTAVSSLATMHEDICHVADDKRVTMIILPFHRNWRMVGGEE------------- 271
           RV VRP TAVS L TMHEDICH+AD KRVTMIILPFH+ W    G               
Sbjct: 547 RVAVRPITAVSPLPTMHEDICHMADTKRVTMIILPFHKRWNADHGHSHHHQDGGGDGNVP 606

Query: 272 ENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQTPG-PGSLVAVGQRICVVFFGGPD 331
           ENVGHGWR+VNQRVLK+APCSVAVLVDRG G+  AQT    GS V   +R+CV+FFGGPD
Sbjct: 607 ENVGHGWRLVNQRVLKNAPCSVAVLVDRGLGSIEAQTLSLDGSNVV--ERVCVIFFGGPD 666

Query: 332 DREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNVILRPLHSKSGDNHYSFSTAPINR 391
           DRE++ELGGRMAEHPAVK TV+RF     + +  + V LRP  SK  + +Y+F T  ++ 
Sbjct: 667 DRESIELGGRMAEHPAVKVTVIRF--LVRETLRSTAVTLRPAPSKGKEKNYAFLTTNVDP 726

Query: 392 EREKELDDVAIAEFRSKWDATVEYTEREANNMNMMVEGVVSIRKEGGYDLIVVGKGRVPS 451
           E+EKELD+ A+ +F+SKW   VEY E+E NN   ++E ++SI +   +DLIVVG+GR+PS
Sbjct: 727 EKEKELDEGALEDFKSKWKEMVEYKEKEPNN---IIEEILSIGQSKDFDLIVVGRGRIPS 786

Query: 452 SMVAKLADRAAEHAELGPVGDILASSGRGIVSSILVIQQHERGAHSEE---TPILNKQPL 493
           + VA LA+R AEH ELGP+GD+LASS   I+ SILV+QQH + AH E+   + I+++  L
Sbjct: 787 AEVAALAERQAEHPELGPIGDVLASSINHIIPSILVVQQHNK-AHVEDITVSKIVSESSL 838

BLAST of Lag0012503 vs. TAIR 10
Match: AT3G17630.1 (cation/H+ exchanger 19 )

HSP 1 Score: 282.7 bits (722), Expect = 5.6e-76
Identity = 180/427 (42.15%), Postives = 254/427 (59.48%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTRTHRKLRDLTAD 113
           L VL  G++  VLND+ FAILVLMALFTTFITTP VM +YKPAR G+   HR     T  
Sbjct: 390 LIVLNIGKDRKVLNDQAFAILVLMALFTTFITTPIVMLIYKPARKGAPYKHR-----TIQ 449

Query: 114 DSNSDDELRILACLHSSDNVPSLISLTESTRNT-KNSSLKLFVMHLVELTKRSSSIMMVQ 173
             + D ELRILAC HS+ N+P+LI+L ES+R T K   L ++ MHL+EL++RSS+I MV 
Sbjct: 450 RKDHDSELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVH 509

Query: 174 RARKNGFPFFARFRKGEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADDK 233
           +AR NG P + +  +    DQM  AF+AY  L  V VRP TA+S L+++HEDIC  A  K
Sbjct: 510 KARNNGLPIWNKIERST--DQMVIAFEAYQHLRAVAVRPMTAISGLSSIHEDICTSAHQK 569

Query: 234 RVTMIILPFHRNWRMVGGEEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQTPGP 293
           RV MI+LPFH++ RM  G  E++GH +  VNQRVL+ APCSV +LVDRG G       G 
Sbjct: 570 RVAMILLPFHKHQRM-DGAMESIGHRFHEVNQRVLQRAPCSVGILVDRGLG-------GT 629

Query: 294 GSLVA--VGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNVIL 353
             +VA  V  ++ + FFGG DDREAL  G +M EHP +  TV +F               
Sbjct: 630 SQVVASEVAYKVVIPFFGGLDDREALAYGMKMVEHPGITLTVYKF--------------- 689

Query: 354 RPLHSKSGDNHYSFSTAPINREREKELDDVAIAEFRS--KWDATVEYTEREANNMNMMVE 413
             + ++     +  S      ++EKE D+  + E  +  + + ++ Y ER   + + ++ 
Sbjct: 690 --VAARGTLKRFEKSEHDEKEKKEKETDEEFVRELMNDPRGNESLAYEERVVESKDDIIA 749

Query: 414 GVVSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILVI 473
            + S+ K    +L VVG+    +S+V     ++ +  ELGPVG +L+SS     +S+LV+
Sbjct: 750 TLKSMSK---CNLFVVGRNAAVASLV-----KSTDCPELGPVGRLLSSSEFSTTASVLVV 776

Query: 474 QQHERGA 476
           Q ++  A
Sbjct: 810 QGYDPAA 776

BLAST of Lag0012503 vs. TAIR 10
Match: AT5G41610.2 (cation/H+ exchanger 18 )

HSP 1 Score: 275.8 bits (704), Expect = 6.9e-74
Identity = 177/421 (42.04%), Postives = 247/421 (58.67%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTRTHRKLRDLTAD 113
           L VL  G++  VLND+ FAI+VLMALFTTFITTP VMAVYKPAR        K R +  +
Sbjct: 321 LIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEGEYKHRAVERE 380

Query: 114 DSNSDDELRILACLHSSDNVPSLISLTESTRN-TKNSSLKLFVMHLVELTKRSSSIMMVQ 173
           ++N+  +LRIL C H + ++PS+I+L E++R   K   L ++ +HL EL++RSS+I+MV 
Sbjct: 381 NTNT--QLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSERSSAILMVH 440

Query: 174 RARKNGFPFFARFRKGEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADDK 233
           + RKNG PF+ R       DQ+  AFQA+ QL RV VRP TA+SS++ +HEDIC  A  K
Sbjct: 441 KVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSRVNVRPMTAISSMSDIHEDICTTAVRK 500

Query: 234 RVTMIILPFHRNWRMVGGEEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQTPGP 293
           +  ++ILPFH++ ++ G  E   G  +R VN+RVL  APCSV + VDRG G +       
Sbjct: 501 KAAIVILPFHKHQQLDGSLETTRG-DYRWVNRRVLLQAPCSVGIFVDRGLGGSSQV---- 560

Query: 294 GSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNVILRP 353
            S   V   + V+FFGGPDDREAL  G RMAEHP +  TV RF  SP    E  NV    
Sbjct: 561 -SAQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPERVGEIVNV---- 620

Query: 354 LHSKSGDNHYSFSTAPINREREKELDDVAIAEFR--SKWDATVEYTEREANNMNMMVEGV 413
               S +N+ + S       +  + D+  ++E R  S  D +V++ E++  N  + V   
Sbjct: 621 --EVSNNNNENQSV------KNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSA 680

Query: 414 VSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILVIQQ 472
           +   +     L+    GR+P   +A      +E  ELGPVG +L S      +S+LVIQQ
Sbjct: 681 IEEVRRSNLFLV----GRMPGGEIALAIRENSECPELGPVGSLLISPESSTKASVLVIQQ 717

BLAST of Lag0012503 vs. TAIR 10
Match: AT5G41610.1 (cation/H+ exchanger 18 )

HSP 1 Score: 275.8 bits (704), Expect = 6.9e-74
Identity = 177/421 (42.04%), Postives = 247/421 (58.67%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTRTHRKLRDLTAD 113
           L VL  G++  VLND+ FAI+VLMALFTTFITTP VMAVYKPAR        K R +  +
Sbjct: 389 LIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEGEYKHRAVERE 448

Query: 114 DSNSDDELRILACLHSSDNVPSLISLTESTRN-TKNSSLKLFVMHLVELTKRSSSIMMVQ 173
           ++N+  +LRIL C H + ++PS+I+L E++R   K   L ++ +HL EL++RSS+I+MV 
Sbjct: 449 NTNT--QLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSERSSAILMVH 508

Query: 174 RARKNGFPFFARFRKGEWHDQMAGAFQAYSQLGRVKVRPTTAVSSLATMHEDICHVADDK 233
           + RKNG PF+ R       DQ+  AFQA+ QL RV VRP TA+SS++ +HEDIC  A  K
Sbjct: 509 KVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSRVNVRPMTAISSMSDIHEDICTTAVRK 568

Query: 234 RVTMIILPFHRNWRMVGGEEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQTPGP 293
           +  ++ILPFH++ ++ G  E   G  +R VN+RVL  APCSV + VDRG G +       
Sbjct: 569 KAAIVILPFHKHQQLDGSLETTRG-DYRWVNRRVLLQAPCSVGIFVDRGLGGSSQV---- 628

Query: 294 GSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNVILRP 353
            S   V   + V+FFGGPDDREAL  G RMAEHP +  TV RF  SP    E  NV    
Sbjct: 629 -SAQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPERVGEIVNV---- 688

Query: 354 LHSKSGDNHYSFSTAPINREREKELDDVAIAEFR--SKWDATVEYTEREANNMNMMVEGV 413
               S +N+ + S       +  + D+  ++E R  S  D +V++ E++  N  + V   
Sbjct: 689 --EVSNNNNENQSV------KNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSA 748

Query: 414 VSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILVIQQ 472
           +   +     L+    GR+P   +A      +E  ELGPVG +L S      +S+LVIQQ
Sbjct: 749 IEEVRRSNLFLV----GRMPGGEIALAIRENSECPELGPVGSLLISPESSTKASVLVIQQ 785

BLAST of Lag0012503 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 246.5 bits (628), Expect = 4.5e-65
Identity = 164/445 (36.85%), Postives = 246/445 (55.28%), Query Frame = 0

Query: 54  LFVLAAGQNLLVLNDEVFAILVLMALFTTFITTPTVMAVYKPARGGSTRTHRKLRDLTAD 113
           + VL  G++  VL+DE FA +VL+AL  T + TP V  +YKP +   +   R     T  
Sbjct: 393 MIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIVTILYKPVKKSVSYKRR-----TIQ 452

Query: 114 DSNSDDELRILACLHSSDNVPSLISLTESTRNTKNSSLKLFVMHLVELTKRSSSIMMVQR 173
            +  D ELR+L C+H+  NVP++I+L E++  TK S + ++V+HLVELT R+S++++V  
Sbjct: 453 QTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHN 512

Query: 174 ARKNGFPFFARFRKGEWHDQMAGAFQAYSQ-LGRVKVRPTTAVSSLATMHEDICHVADDK 233
            RK+G P  A  R     D +  AF+ Y Q    V V+P TA+S  +TMHED+C +A+DK
Sbjct: 513 TRKSGRP--ALNRTQAQSDHIINAFENYEQHAAFVAVQPLTAISPYSTMHEDVCSLAEDK 572

Query: 234 RVTMIILPFHRNWRMVGGEEENVGHGWRVVNQRVLKSAPCSVAVLVDRGFGANGAQTPGP 293
           RV+ II+PFH+  + V G  E+    +R+VNQ +L+++PCSV +LVDRG   NGA     
Sbjct: 573 RVSFIIIPFHKQ-QTVDGGMESTNPAYRLVNQNLLENSPCSVGILVDRGL--NGATRLNS 632

Query: 294 GSLVAVGQRICVVFFGGPDDREALELGGRMAEHPAVKGTVVRFRPSPSDGVEGSNVILRP 353
            +   V  ++ V+FFGGPDDREAL    RMA+HP +  TV+RF     +    S      
Sbjct: 633 NT---VSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDEDEADTAST----- 692

Query: 354 LHSKSGDNHYSFSTAPINREREKELDDVAIAEFRSK--WDATVEYTEREANNMNMMVEGV 413
                  N        ++  ++++LDD  I  FR++     ++ Y E+  +N    V  V
Sbjct: 693 ----RATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVAAV 752

Query: 414 VSIRKEGGYDLIVVGKGRVPSSMVAKLADRAAEHAELGPVGDILASSGRGIVSSILVIQQ 473
            S+  +  +DL +VG+G   SS +       +E  ELG +GD+LASS      S+LV+QQ
Sbjct: 753 RSM--DSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQ 812

Query: 474 H-------------ERGAHSEETPI 483
           +             E   HS ET +
Sbjct: 813 YVGSWAQEDDMDFPESPVHSHETKV 813