Lag0012457 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0012457 vs. NCBI nr
Match: XP_038881205.1 (protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida] >XP_038881206.1 protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 959.9 bits (2480), Expect = 9.8e-276
Identity = 484/571 (84.76%), Postives = 520/571 (91.07%), Query Frame = 0

Query: 8   HSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHSAYVFQ 67
           HSSSD P  K+A GGWRAVRFIL NETFEKLASMSLI NLVLYLHTMYNLDNV SAYVFQ
Sbjct: 7   HSSSDAPRKKTARGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQ 66

Query: 68  IWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGES 127
           IWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVL+LGAGLPQMRPPPC +GES
Sbjct: 67  IWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLSLGAGLPQMRPPPCISGES 126

Query: 128 NCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFL 187
           +CPQP PWQL FLYLG GL+VVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWY L
Sbjct: 127 SCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLL 186

Query: 188 FSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSVFADMA 247
           FSIAL+IALT+VVYVQTNISWTLGFAIPTA F+ S+L+FLSGRRFY+CKAPQGSVF+D+A
Sbjct: 187 FSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIFLSGRRFYVCKAPQGSVFSDLA 246

Query: 248 KVIVATCHKRGIPKPSP-DDLHSPPMDSDSSKLAHTDRFLVFDKAATVVDSATELDEEGR 307
           KV+VA   KR IPKP+  D LH+PPM  +S KLAHTDRFLVFDKAATVVDS+ ELDEEG+
Sbjct: 247 KVVVAMFRKRKIPKPNQIDHLHNPPM--NSCKLAHTDRFLVFDKAATVVDSSIELDEEGK 306

Query: 308 SKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSLGPHFQI 367
           SK+EWRLCSVHQVEQFKC+VGI+P+W AGIACFISMQQM SFGILQAIQMNR +GPHFQI
Sbjct: 307 SKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQMGSFGILQAIQMNRFIGPHFQI 366

Query: 368 PPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGIL 427
           PPAWMSLTPMIALSIWIYIYEKYVEFMK+KTH NKR SMK RI+IGIVMSVLCMVVAG+L
Sbjct: 367 PPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSMKTRIEIGIVMSVLCMVVAGLL 426

Query: 428 EKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAI 487
           EK+RRD+A+ENKSFVSPLHV +LIPEF LSGLTEAFAAIAVMELLTTHLPESLRTVAGAI
Sbjct: 427 EKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAI 486

Query: 488 FFLSLSLASYLSSVIMGILSKISK----KSPWLGGNDLNKNRLDYFFYVVAVIAGLNFFY 547
           FFLSLSLASYLSSV+MGI+SKI +    +  WLGGNDLNKNRLDYFFYVV  IA LNFFY
Sbjct: 487 FFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLNKNRLDYFFYVVGGIAALNFFY 546

Query: 548 FRLVAAKFLPKGDVDPRQKAGERQLEDEESG 574
           FR  AA FLP  DVDPRQKA +RQLED E G
Sbjct: 547 FRFFAAGFLPSPDVDPRQKAHQRQLEDAELG 575

BLAST of Lag0012457 vs. NCBI nr
Match: XP_023518809.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 954.1 bits (2465), Expect = 5.4e-274
Identity = 479/578 (82.87%), Postives = 527/578 (91.18%), Query Frame = 0

Query: 1   MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 60
           M+NG+L  SS DP P K   GGWRAVRFILANETFEKLASMSLIANL+LYLHTMYNLDNV
Sbjct: 1   MDNGTL-RSSLDPSP-KPPLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 61  HSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 120
            SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 121 PCTTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC++G+++C Q   WQLAFLYLG GLLV+GAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQG 240
           LNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAFIFS+++FL GR  YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 241 SVFADMAKVIVATCHKRGIPKPSPDDLHSPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 300
           SVFADMAKV++AT  KR IP+PSP+ LH PPM  DSSKL HT+RFLVFDKAA V+DS+ E
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNRFLVFDKAAMVIDSSME 300

Query: 301 LDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSL 360
           LDE+GRSKNEW+LC+VHQVEQ KC+VGIVPIWAAGI+CFISMQQM SFGILQAIQMNR L
Sbjct: 301 LDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFL 360

Query: 361 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCM 420
           GPHF+IPPAWMSL PMIALS+WIYIYEKYVEFMKRKTHHNKR SMK RI+IGIVMSVLCM
Sbjct: 361 GPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMKRKTHHNKRFSMKMRIEIGIVMSVLCM 420

Query: 421 VVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 480
           VVAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAAI+VMELLTT LPESLR
Sbjct: 421 VVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLR 480

Query: 481 TVAGAIFFLSLSLASYLSSVIMGILSKISK----KSPWLGGNDLNKNRLDYFFYVVAVIA 540
           TVAGAIFFLSLSLASYLSSV+MGI++++SK    K+ WL GNDLNKN LDYFFYVV VIA
Sbjct: 481 TVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIA 540

Query: 541 GLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGY 575
            LNFFYFR V  +FLP+GDVDPR KAGER+ +DEESG+
Sbjct: 541 ALNFFYFRFVGTRFLPEGDVDPRGKAGERRSDDEESGF 574

BLAST of Lag0012457 vs. NCBI nr
Match: KAG6595347.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027355.1 Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 951.4 bits (2458), Expect = 3.5e-273
Identity = 477/578 (82.53%), Postives = 526/578 (91.00%), Query Frame = 0

Query: 1   MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 60
           M+NG+L  SS DP P K   GGWRAVRFILANETFEKLASMSLIANL+LYLHTMYNLDNV
Sbjct: 1   MDNGTL-RSSLDPSP-KPPLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 61  HSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 120
            SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 121 PCTTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC++G+++C Q   WQLAFLYLG GLLV+GAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQG 240
           LNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAFIFS+++FL GR  YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 241 SVFADMAKVIVATCHKRGIPKPSPDDLHSPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 300
           SVFADMAKV++AT  KR IP+PSP+ LH PPM  DSSKL HT+RFLVFDKAA V+DS+ E
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNRFLVFDKAAMVIDSSME 300

Query: 301 LDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSL 360
           LDE+GRSKNEW+LC+VHQVEQ KC+VGIVPIWAAGI+CFISMQQM SFGILQAIQMNR L
Sbjct: 301 LDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFL 360

Query: 361 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCM 420
           GPHF+IPPAWMSL PMIALS+WIYIYEKYVEFM+RKTHHNKR SMK RI+IGIVMSVLCM
Sbjct: 361 GPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCM 420

Query: 421 VVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 480
           +VAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAAI+VMELLTT LPESLR
Sbjct: 421 IVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLR 480

Query: 481 TVAGAIFFLSLSLASYLSSVIMGILSKISK----KSPWLGGNDLNKNRLDYFFYVVAVIA 540
           TVAGAIFFLSLSLASYLSSV+MGI++++SK    K+ WL GNDLNKN LDYFFYVV VIA
Sbjct: 481 TVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIA 540

Query: 541 GLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGY 575
            LNFFYFR V  +FLP+GDVDPR KA ER+ EDEESG+
Sbjct: 541 ALNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGF 574

BLAST of Lag0012457 vs. NCBI nr
Match: XP_022931895.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita moschata])

HSP 1 Score: 951.4 bits (2458), Expect = 3.5e-273
Identity = 477/578 (82.53%), Postives = 526/578 (91.00%), Query Frame = 0

Query: 1   MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 60
           M+NG+L  SS DP P K   GGWRAVRFILANETFEKLASMSLIANL+LYLHTMYNLDNV
Sbjct: 1   MDNGTL-RSSLDPSP-KPQLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 61  HSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 120
            SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 121 PCTTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC++G+++C Q   WQLAFLYLG GLLV+GAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQG 240
           LNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAFIFS+++FL GR  YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 241 SVFADMAKVIVATCHKRGIPKPSPDDLHSPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 300
           SVFADMAKV++AT  KR IP+PSP+ LH PPM  DSSKL HT+RFLVFDKAA V+DS+ E
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNRFLVFDKAAMVIDSSME 300

Query: 301 LDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSL 360
           LDE+GRSKNEW+LC+VHQVEQ KC+VGIVPIWAAGI+CFISMQQM SFGILQAIQMNR L
Sbjct: 301 LDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFL 360

Query: 361 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCM 420
           GPHF+IPPAWMSL PMIALS+WIYIYEKYVEFM+RKTHHNKR SMK RI+IGIVMSVLCM
Sbjct: 361 GPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCM 420

Query: 421 VVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 480
           +VAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAAI+VMELLTT LPESLR
Sbjct: 421 IVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLR 480

Query: 481 TVAGAIFFLSLSLASYLSSVIMGILSKISK----KSPWLGGNDLNKNRLDYFFYVVAVIA 540
           TVAGAIFFLSLSLASYLSSV+MGI++++SK    K+ WL GNDLNKN LDYFFYVV VIA
Sbjct: 481 TVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIA 540

Query: 541 GLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGY 575
            LNFFYFR V  +FLP+GDVDPR KA ER+ EDEESG+
Sbjct: 541 ALNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGF 574

BLAST of Lag0012457 vs. NCBI nr
Match: XP_004142186.1 (protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KAE8649550.1 hypothetical protein Csa_017951 [Cucumis sativus])

HSP 1 Score: 938.3 bits (2424), Expect = 3.1e-269
Identity = 472/574 (82.23%), Postives = 510/574 (88.85%), Query Frame = 0

Query: 1   MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 60
           MENG+L H+S D   +K   GGWRAVRFIL NETFEKLASMSLI NLVLYLHTMYNLDNV
Sbjct: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60

Query: 61  HSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 120
            SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGS+AS LGMGVLTLGAGLPQMRPP
Sbjct: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120

Query: 121 PCTTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC +GESNCPQP PWQL FLYLG GL+V+GAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQG 240
           LNWWYFLFSI+L+IALT+VVYVQTNISWTLGF IPTA F+FS+ +FL G R+YICKAPQG
Sbjct: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240

Query: 241 SVFADMAKVIVATCHKRGIPKPSP-DDLHSPPMDSDSSKLAHTDRFLVFDKAATVVDSAT 300
           SVFADMAKV+VATC K  IPK +  D LH+PPM  +SSKLAHT+RFL+FDKAATVVDS+ 
Sbjct: 241 SVFADMAKVVVATCRKWQIPKTTQFDQLHNPPM--NSSKLAHTNRFLIFDKAATVVDSSV 300

Query: 301 ELDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRS 360
           ELDEEG+SKNEWRLCSVHQVEQFKC+VGI P+W AGI CF+SMQQMSSFGILQAIQMNRS
Sbjct: 301 ELDEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRS 360

Query: 361 LGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLC 420
           +GPHFQIPPAWMSLTPMIALSIWIYIYEKYVE MK+KT  NKR SMK RI+IGIVMSVLC
Sbjct: 361 IGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLC 420

Query: 421 MVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESL 480
           MVVAG+LEK RRD+A+ENKSF+SPLHV VLIPEF LSGLTEAF AIAVMELLTTHLPESL
Sbjct: 421 MVVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESL 480

Query: 481 RTVAGAIFFLSLSLASYLSSVIMGILSKISKKSPWLGGNDLNKNRLDYFFYVVAVIAGLN 540
           RTVAGAIFF SLSLASYLSSV+ GI+  + ++  WLGGNDLNKNRLDYFF VV VIA LN
Sbjct: 481 RTVAGAIFFFSLSLASYLSSVLTGIVRGVERE--WLGGNDLNKNRLDYFFSVVGVIAALN 540

Query: 541 FFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESG 574
           FFYFR  A  FLPK DVDP  KA + QLED E G
Sbjct: 541 FFYFRFFAVAFLPKPDVDPSHKAHQMQLEDAELG 570

BLAST of Lag0012457 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 564.7 bits (1454), Expect = 1.2e-159
Identity = 288/554 (51.99%), Postives = 385/554 (69.49%), Query Frame = 0

Query: 6   LDHSSSDPPPS---KSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHS 65
           +D  SS P      K   GGWRA+++I+ANE+FEKLASMSLI NL +YL T YNL  V  
Sbjct: 1   MDVESSSPSSHALIKKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFL 60

Query: 66  AYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPC 125
             V  IW G+ N   LAGAF++DAYLGRF+TLL GSIASF+GMG+  L A LP +RP  C
Sbjct: 61  VNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDAC 120

Query: 126 TTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLN 185
               +   QP  WQL  L+ G GLL +GAGG+RPCNI+FGADQFDT+T+KG+A LE+F N
Sbjct: 121 IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFN 180

Query: 186 WWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSV 245
           WWYF F++AL+IALT VVY+QTNISW +GF IPTA    S+  F+ G+  YIC   +GSV
Sbjct: 181 WWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSV 240

Query: 246 FADMAKVIVATCHKRGIPKPSPDDLH--SPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 305
           FAD+ KV+ A C KR + KP  D      P  D   + L      L F   A++V +  E
Sbjct: 241 FADIVKVVTAACKKRKV-KPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNPNE 300

Query: 306 LDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSL 365
           L+E+G +K +WRLCSV QV+  KC+  I+P+W  GIACFI   Q + +GILQA+QM+++ 
Sbjct: 301 LNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTF 360

Query: 366 GPH-FQIPPAWMSLTPMIALSIWIYIYE-KYVEFMKRKTHHNKRLSMKRRIDIGIVMSVL 425
           GPH FQ+P  WM+L  MI L+IWI +YE   +  +K+ T   KRL++K RI+  IVM ++
Sbjct: 361 GPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGII 420

Query: 426 CMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPES 485
           CM+VAG  EK RR SAL+N SFVSP+ + +L+P+F L+GLTEAF+A+A+ME LT  +PE 
Sbjct: 421 CMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEH 480

Query: 486 LRTVAGAIFFLSLSLASYLSSVIMGILSKISKK--SPWLGGNDLNKNRLDYFFYVVAVIA 545
           +R VAGAIFFLS S+ASY+ ++++ ++  +++K    WLG  DLNKNRL+ +F+++A I 
Sbjct: 481 MRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEGKSWLGDKDLNKNRLENYFFIIAGIQ 540

Query: 546 GLNFFYFRLVAAKF 551
             N  YFRL A+++
Sbjct: 541 VANLLYFRLFASRY 551

BLAST of Lag0012457 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 9.7e-117
Identity = 228/569 (40.07%), Postives = 358/569 (62.92%), Query Frame = 0

Query: 21  GGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHSAYVFQIWGGTTNFAPLAG 80
           GGWRAV FIL NET E+L S+ L+AN ++YL  +++L+ V +A V  IW G TN  PL G
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 81  AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGES-NCPQPHPWQLAF 140
           A+++D Y+GRF T+ F S A+ LG+  +TL A  PQ+ P  C + +  +C  P+  Q+  
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

Query: 141 LYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLV 200
           L LG   L VG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  F++ LII  T+V
Sbjct: 173 LLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVV 232

Query: 201 VYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGI 260
           VY+Q  +SW +GF+IPT     +V++F +G + Y+   P+GS+F+ +A+VIVA   KR +
Sbjct: 233 VYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKL 292

Query: 261 PKPSPDD----LHSPPMDSD-SSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNEWRLC 320
             P+ DD     + P + S   SKL  +++F   DKAA V++   +L  EG   ++WRLC
Sbjct: 293 KLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPEGPPADKWRLC 352

Query: 321 SVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSLGPHFQIPPAWMSLT 380
           SV +VE+ KCL+ IVPIW+AGI    +M    +F + QA++M+R+LGP F+IP   +S+ 
Sbjct: 353 SVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVI 412

Query: 381 PMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEKVRRDS 440
            ++ + I++  Y++ +V FM+R T H   +++ +RI  GIV ++  M+VAGI+E++RR  
Sbjct: 413 SLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIR 472

Query: 441 ALE--NKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSL 500
           ++   + + ++P+ V  L P+ +L GL EAF  I  +E   +  PE +R++A ++F LS 
Sbjct: 473 SINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSF 532

Query: 501 SLASYLSSVIMGILSKIS---KKSPWLGGNDLNKNRLDYFFYVVAVIAGLNFFYFRLVAA 560
           + +SYLSS ++ ++ K S    +  WL  N LN  +LDYF+Y++AV+  +N  YF   A 
Sbjct: 533 AGSSYLSSFLVTVVHKFSGGHDRPDWLNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYCAR 592

Query: 561 KF-----LPKGDVDPRQKAGERQLEDEES 573
            +     LP  D +  + + + ++  ++S
Sbjct: 593 GYRYKVGLPIEDFEEDKSSDDVEMTSKKS 618

BLAST of Lag0012457 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 421.4 bits (1082), Expect = 1.7e-116
Identity = 236/566 (41.70%), Postives = 351/566 (62.01%), Query Frame = 0

Query: 21  GGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHSAYVFQIWGGTTNFAPLAG 80
           GGWRA+ FIL NET EKL S+ + AN +LYL  +++++ V +  V+ +W G TNFAPL G
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 81  AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGE-SNCPQPHPWQLAF 140
           A ++DAY+GRF T+ + S+ S LG+  +TL A LPQ+ PPPC       C  P+  QL  
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

Query: 141 LYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLV 200
           L+LG G L +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ LI + T+V
Sbjct: 136 LFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVV 195

Query: 201 VYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGI 260
           VY+QT +SW +GF+IPT+    +V++F  G RFY+   P+GSVF+ +A+VIVA   KR +
Sbjct: 196 VYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL 255

Query: 261 PKPSPDD----LHSPPMDSD-SSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNEWRLC 320
                DD     + PP+     SKL  TD+F   DKAA ++D   +L  EG   N+WRLC
Sbjct: 256 KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLC 315

Query: 321 SVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSLGPHFQIPPAWMSLT 380
           S+ +VE+ KCL+ +VP+W+AGI   ++M   ++F + QA +M+R +GPHF+IP A +++ 
Sbjct: 316 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVI 375

Query: 381 PMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEKVRRDS 440
             I + IW+ IYE   V F+ R      R+++ +R+ IGIV ++L M  AG +E VRR  
Sbjct: 376 SYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTR 435

Query: 441 ALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSL 500
           A E    ++ + V  L    +L GL E+F  I ++E   +  PE +R++A ++F LS + 
Sbjct: 436 ATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAA 495

Query: 501 ASYLSSVIMGILSKIS--KKSP-WLGGNDLNKNRLDYFFYVVAVIAGLNFFYFRLVAAKF 560
           A+YLSS+++  + K+S  K  P WL   DL++ +LDYF+Y++AV+  +N  YF   A ++
Sbjct: 496 ANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCAHRY 555

Query: 561 LPKGDVDPRQKAGERQLEDEESGYSH 577
             K          E+ L D E    H
Sbjct: 556 QYKAGSQIEDFNEEKSLLDIEPNQRH 571

BLAST of Lag0012457 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.9e-104
Identity = 217/561 (38.68%), Postives = 337/561 (60.07%), Query Frame = 0

Query: 17  KSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHSAYVFQIWGGTTNFA 76
           K  + GW+ + FI+ NETFEKL  +  ++NL++YL  ++NL ++ +A +   + GT NF 
Sbjct: 41  KVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFG 100

Query: 77  PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPC-TTGESNCPQPHPW 136
               AFL D Y GR+ TL    IA FLG  V+ L A +PQ+ P  C T  +S C  P   
Sbjct: 101 TFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGG 160

Query: 137 QLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIA 196
           Q+AFL +G G LVVGAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ A I++
Sbjct: 161 QIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILS 220

Query: 197 LTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCH 256
           LTLVVYVQ+N+SWT+G  IP      + L+F +G + Y+     GS  A +A+VI     
Sbjct: 221 LTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIK 280

Query: 257 KRGIPKPSPDDL-----HSPPMDSDSSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNE 316
           KRG+ KP+         + PP  + +SKL +TD+F   DKAA ++    +L  +G+  + 
Sbjct: 281 KRGL-KPAKQPWLNLYNYYPPKYA-NSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADP 340

Query: 317 WRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSLGP-HFQIPPA 376
           W+LC++ QVE+ KC+V ++PIW A    ++++ Q  ++ + QA+Q +R LG   F IP A
Sbjct: 341 WKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAA 400

Query: 377 WMSLTPMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEK 436
              +  M  ++++I +Y++  V  M+R T  +  +++ +RI  GI  +   +VVAG +E+
Sbjct: 401 TYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEE 460

Query: 437 VRRDSAL--------ENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 496
            RR  AL          K  +S +    LIP+  L+G+ EAFAAI  ME      PE++R
Sbjct: 461 RRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMR 520

Query: 497 TVAGAIFFLSLSLASYLSSVIMGILSKISKKSPWLGGN----DLNKNRLDYFFYVVAVIA 556
           + AG+IF++   ++SYL S ++  + + ++ S   GGN    DLNK RLD F++++A I 
Sbjct: 521 SFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSS--GGNWLAEDLNKGRLDLFYFMIAGIL 580

Query: 557 GLNFFYFRLVAAKFLPKGDVD 558
            +NF YF +++  +  KG  D
Sbjct: 581 AVNFAYFLVMSRWYRYKGSDD 596

BLAST of Lag0012457 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.4e-102
Identity = 209/550 (38.00%), Postives = 334/550 (60.73%), Query Frame = 0

Query: 9   SSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHSAYVFQI 68
           SSSD    +    GW+A+ +I+ NET E+LA+  L+AN ++Y+   Y++D V +  +   
Sbjct: 8   SSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINT 67

Query: 69  WGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGE-- 128
           W   TNFAP+ GAF++D+Y G+F T++FGSIA  LGM VLT  + +P +RPPPCT  +  
Sbjct: 68  WSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQIT 127

Query: 129 SNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYF 188
             C      QL  L  G  LL VG GGIR C++ F  DQFD +TE+GR    SF +W+Y 
Sbjct: 128 GQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYT 187

Query: 189 LFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSVFADM 248
             +I  ++++TLV+YVQ NISW +GFAIPT    F++L+   G R+Y+   P+GSVF+ +
Sbjct: 188 THTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGV 247

Query: 249 AKVIVATCHKRGIPKPSPDDLHSPPMDSD--SSKLAHTDRFLVFDKAATVVDSATELDEE 308
            KV+VA   KR     S  D H P +++D  S+KL  TD+F   +KA  V+++    DE 
Sbjct: 248 FKVLVAAYKKRKARFTSGIDYHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNN----DEA 307

Query: 309 GRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSL-GPH 368
           G    EWR C+V Q+E  K ++ I+PI+A+ I  F++M Q  +F + QA++M+    G  
Sbjct: 308 G--NEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTS 367

Query: 369 FQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVV 428
           + IPPA +++  ++ + IW+  YE   V  ++  T  N  +S+ +++ IG + S+  M++
Sbjct: 368 YLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLI 427

Query: 429 AGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTV 488
           +GI+E+ RRD +L        + V  L P+ VL G  + F  + + E     +P ++R++
Sbjct: 428 SGIVERKRRDLSLNGVK----MSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSI 487

Query: 489 AGAIFFLSLSLASYLSSVIMGILSKISKK--SPWLGGNDLNKNRLDYFFYVVAVIAGLNF 548
             ++ +L LSLASYLSS ++ I+  ++ +    WL  +D++K++LD F+Y +A ++ LNF
Sbjct: 488 GNSLLYLGLSLASYLSSAMVSIVHSVTARGGQSWL-TDDIDKSKLDCFYYFIAALSTLNF 546

Query: 549 FYFRLVAAKF 551
            +F   A ++
Sbjct: 548 IFFFWCARRY 546

BLAST of Lag0012457 vs. ExPASy TrEMBL
Match: A0A6J1F0P7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438173 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.7e-273
Identity = 477/578 (82.53%), Postives = 526/578 (91.00%), Query Frame = 0

Query: 1   MENGSLDHSSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNV 60
           M+NG+L  SS DP P K   GGWRAVRFILANETFEKLASMSLIANL+LYLHTMYNLDNV
Sbjct: 1   MDNGTL-RSSLDPSP-KPQLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 61  HSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPP 120
            SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 121 PCTTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC++G+++C Q   WQLAFLYLG GLLV+GAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQG 240
           LNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAFIFS+++FL GR  YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 241 SVFADMAKVIVATCHKRGIPKPSPDDLHSPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 300
           SVFADMAKV++AT  KR IP+PSP+ LH PPM  DSSKL HT+RFLVFDKAA V+DS+ E
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNRFLVFDKAAMVIDSSME 300

Query: 301 LDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSL 360
           LDE+GRSKNEW+LC+VHQVEQ KC+VGIVPIWAAGI+CFISMQQM SFGILQAIQMNR L
Sbjct: 301 LDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFL 360

Query: 361 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCM 420
           GPHF+IPPAWMSL PMIALS+WIYIYEKYVEFM+RKTHHNKR SMK RI+IGIVMSVLCM
Sbjct: 361 GPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCM 420

Query: 421 VVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 480
           +VAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAAI+VMELLTT LPESLR
Sbjct: 421 IVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLR 480

Query: 481 TVAGAIFFLSLSLASYLSSVIMGILSKISK----KSPWLGGNDLNKNRLDYFFYVVAVIA 540
           TVAGAIFFLSLSLASYLSSV+MGI++++SK    K+ WL GNDLNKN LDYFFYVV VIA
Sbjct: 481 TVAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIA 540

Query: 541 GLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGY 575
            LNFFYFR V  +FLP+GDVDPR KA ER+ EDEESG+
Sbjct: 541 ALNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGF 574

BLAST of Lag0012457 vs. ExPASy TrEMBL
Match: A0A6J1DBD9 (protein NRT1/ PTR FAMILY 2.8-like OS=Momordica charantia OX=3673 GN=LOC111019435 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.7e-252
Identity = 446/583 (76.50%), Postives = 500/583 (85.76%), Query Frame = 0

Query: 1   MENGSLDHSSSDPP---PSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNL 60
           MEN ++DHSS DPP   PSK+A GGWRAVRFILANETFEKLASMSL+ANL+LYLHTMYNL
Sbjct: 1   MENDAIDHSSLDPPPVAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60

Query: 61  DNVHSAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQM 120
           DNV SA V  IWGGTTNFAP+AGAFLADAYLGRFYTLLFGSIASF GMGVLTLGAGLP++
Sbjct: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFFGMGVLTLGAGLPEL 120

Query: 121 RPPPCTTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKL 180
           RPP C+  ++ CPQP PWQLAFLYLG GLLV+GAGGIRPCNISFGADQFDT T KGRAKL
Sbjct: 121 RPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180

Query: 181 ESFLNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKA 240
           ESFLNWWYFLFSIALI+ALT+VVY+QTNISW  GFAIPTA F+ S+L+FL GR  YI K 
Sbjct: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240

Query: 241 PQGSVFADMAKVIVATCHKRGIPKP-SPDDLHSPPMD-SDSSKLAHTDRFLVFDKAATVV 300
           PQGSVFADMAKVI+ATC K  + KP + ++LH PP    DS+KL HT+RFLVFDKAATVV
Sbjct: 241 PQGSVFADMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300

Query: 301 DSATELDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQ 360
           D   ELDE G S + WRLCSV QVEQ KC++GIVP+W AGI+CFI+MQQM SFG+LQAIQ
Sbjct: 301 DPVAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360

Query: 361 MNRSLGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLSMKRRIDIGIVM 420
           M+R LG HF+IPPAWM+LTPMIALSIWI++YEKYV  MKRKT  NKR+SM+RRI+IGIVM
Sbjct: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420

Query: 421 SVLCMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHL 480
           SV+CMVV+GI EK+RRD A+ N SFV+ LHV+ L PEF LSGLTEAFAAIAVMELLTTHL
Sbjct: 421 SVVCMVVSGITEKLRRDLAVGNGSFVARLHVTALXPEFALSGLTEAFAAIAVMELLTTHL 480

Query: 481 PESLRTVAGAIFFLSLSLASYLSSVIMGILSKIS-----KKSPWLGGNDLNKNRLDYFFY 540
           PESLRTVAGAIFFLSLSLASYLSSV++GI+SKIS     KK+ W+GGNDLN NRL+YFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540

Query: 541 VVAVIAGLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESG 574
           VVAVI  LNFFYFR  AA FLPK DVD   +  ERQLEDEE+G
Sbjct: 541 VVAVIGTLNFFYFRFFAAGFLPKSDVDDHDQTHERQLEDEETG 583

BLAST of Lag0012457 vs. ExPASy TrEMBL
Match: A0A1S4DX14 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo OX=3656 GN=LOC103491761 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 1.2e-223
Identity = 388/470 (82.55%), Postives = 428/470 (91.06%), Query Frame = 0

Query: 105 MGVLTLGAGLPQMRPPPCTTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGAD 164
           MGVLTLGAGLPQMRPPPC +GESNCPQP PWQL+FLYLG GL+V+GAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 60

Query: 165 QFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVL 224
           QFDTNTEKGRAKLESFLNWWYFLFS+AL+IALT+VVYVQTN+SWTLGF IPTA F+FS+ 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSVALVIALTIVVYVQTNVSWTLGFGIPTACFVFSIS 120

Query: 225 VFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGIPKPSP-DDLHSPPMDSDSSKLAHTD 284
           +FL G R+YICKAPQGSVFAD+AKV+VATC KR IPKP+  D LH+PPM  +SSKLAHT+
Sbjct: 121 IFLLGHRYYICKAPQGSVFADIAKVVVATCRKRQIPKPTQFDQLHNPPM--NSSKLAHTN 180

Query: 285 RFLVFDKAATVVDSATELDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQ 344
           RFL+FDKAATVVDS+ ELDE+G+SKNEWRLCSVHQVE+FKC+VGI+P+W AGI CF+SMQ
Sbjct: 181 RFLIFDKAATVVDSSVELDEDGKSKNEWRLCSVHQVEEFKCVVGIIPVWLAGIPCFMSMQ 240

Query: 345 QMSSFGILQAIQMNRSLGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRL 404
           QM SFGILQAIQMNR +GPHFQIPPAWMSLTPMI LSIWIYIYEKYVE MK+KT  NKR 
Sbjct: 241 QMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMITLSIWIYIYEKYVEHMKKKTRSNKRF 300

Query: 405 SMKRRIDIGIVMSVLCMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFA 464
           SMK RI+IGIVMSVLCMVVAGILEK+RRD+A+ENKSF+SPLHV VLIPEF LSGLTEAFA
Sbjct: 301 SMKTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFA 360

Query: 465 AIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVIMGILSKISKKSPWLGGNDLNKN 524
           AIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSV+ GI+SK+ ++  WLGGNDLNKN
Sbjct: 361 AIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVSKVERE--WLGGNDLNKN 420

Query: 525 RLDYFFYVVAVIAGLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESG 574
           RLDYFFYVV VIA LNFFYFR  A  FLPK DVDPRQKA +RQLED E G
Sbjct: 421 RLDYFFYVVGVIAALNFFYFRFFAGAFLPKPDVDPRQKAHQRQLEDAELG 466

BLAST of Lag0012457 vs. ExPASy TrEMBL
Match: A0A6J1HNI7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita maxima OX=3661 GN=LOC111466257 PE=3 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 2.1e-223
Identity = 388/474 (81.86%), Postives = 431/474 (90.93%), Query Frame = 0

Query: 105 MGVLTLGAGLPQMRPPPCTTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGAD 164
           MGVLTLGA LP+MRPPPC++G+++C Q  PWQLAFLYLG GLLV+GAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAALPEMRPPPCSSGKTDCIQSKPWQLAFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 165 QFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVL 224
           QFDTNTEKGRAKLESFLNWWYFLFSIAL+IALTLVVY+QTNISWTLGFAIPTAAFIFS++
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIV 120

Query: 225 VFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGIPKPSPDDLHSPPMDSDSSKLAHTDR 284
           +FL GR  YIC+ PQGSVFADMAKV++AT  KR IP+PSP+ LH PPM  DSSKL HT+R
Sbjct: 121 IFLLGRHLYICRIPQGSVFADMAKVLIATYRKRQIPRPSPNHLHDPPM--DSSKLPHTNR 180

Query: 285 FLVFDKAATVVDSATELDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQ 344
           FLVFDKAA V DS+ ELDE+GRSKNEW+LC+VHQVEQ KC+VGIVPIWAAGI+CFISMQQ
Sbjct: 181 FLVFDKAAMVTDSSMELDEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQ 240

Query: 345 MSSFGILQAIQMNRSLGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKRLS 404
           M SFGILQAIQMNR LGPHF+IPPAWMSL PMIALS+WIYIYEKYVEFMKRKTHHNKR S
Sbjct: 241 MGSFGILQAIQMNRFLGPHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMKRKTHHNKRFS 300

Query: 405 MKRRIDIGIVMSVLCMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAA 464
           MK RI+IGI+MSVLCMVVAGILEK+RRDSA+EN++FVSPLHVSVLIPEF LSGLTEAFAA
Sbjct: 301 MKMRIEIGIMMSVLCMVVAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAA 360

Query: 465 IAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVIMGILSKISK----KSPWLGGNDL 524
           I+VMELLTT LPESLRTVAGAIFFLSLSLASYLSSV+MGI++++SK    K+ WL GNDL
Sbjct: 361 ISVMELLTTQLPESLRTVAGAIFFLSLSLASYLSSVLMGIVNRVSKTEDSKTSWLEGNDL 420

Query: 525 NKNRLDYFFYVVAVIAGLNFFYFRLVAAKFLPKGDVDPRQKAGERQLEDEESGY 575
           NKN LDYFFYVV VIA LNFFYFR V  +FLP+GDVDPR KA ER+ EDEESG+
Sbjct: 421 NKNHLDYFFYVVGVIAALNFFYFRFVGTRFLPEGDVDPRVKANERRSEDEESGF 472

BLAST of Lag0012457 vs. ExPASy TrEMBL
Match: A0A6J1GFB3 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111453646 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 7.7e-218
Identity = 383/458 (83.62%), Postives = 413/458 (90.17%), Query Frame = 0

Query: 105 MGVLTLGAGLPQMRPPPCTTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGAD 164
           MGVLTLGAG P+MRPPPC  GESNCPQPHPWQL FLYLG GLLV+GAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 165 QFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVL 224
           QFDTNTEKGRAKLESFLNWWY LFS+AL+IALTLVVYVQT+ISWTLGFAIPTA F+FS+L
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSIL 120

Query: 225 VFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGIPKPSPDD--LHSPPMDSDSSKLAHT 284
           +FL GRRFYICK PQGSVFAD+AKV+VATC KR +PK +  D  LH PPM  DSSKL+HT
Sbjct: 121 IFLLGRRFYICKTPQGSVFADIAKVLVATCRKRNVPKFTQIDHFLHDPPM--DSSKLSHT 180

Query: 285 DRFLVFDKAATVVDSATELDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISM 344
           DRFLVFDKAATVVDS+TELDE+G+SKNEW+LCSVHQVEQFKC+VGIVPIWAAGI CFISM
Sbjct: 181 DRFLVFDKAATVVDSSTELDEQGKSKNEWKLCSVHQVEQFKCVVGIVPIWAAGIPCFISM 240

Query: 345 QQMSSFGILQAIQMNRSLGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKRKTHHNKR 404
           QQM SFGILQAIQMNR +GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMK+KTH NKR
Sbjct: 241 QQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKR 300

Query: 405 LSMKRRIDIGIVMSVLCMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAF 464
            S+K RI+IGIVMSVLCMVVAGILE +RR SA ENKSFVSPLHV +LIPEF LSGLTEAF
Sbjct: 301 FSLKTRIEIGIVMSVLCMVVAGILENIRRSSAEENKSFVSPLHVWMLIPEFALSGLTEAF 360

Query: 465 AAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVIMGILSKISK----KSPWLGGN 524
           AAIAVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSV+M I+SKI++    KS WLGGN
Sbjct: 361 AAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGN 420

Query: 525 DLNKNRLDYFFYVVAVIAGLNFFYFRLVAAKFLPKGDV 557
           DLNKNRLDYFFYVV VIA  NFFYFR  A  FLPK +V
Sbjct: 421 DLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 456

BLAST of Lag0012457 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 564.7 bits (1454), Expect = 8.6e-161
Identity = 288/554 (51.99%), Postives = 385/554 (69.49%), Query Frame = 0

Query: 6   LDHSSSDPPPS---KSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHS 65
           +D  SS P      K   GGWRA+++I+ANE+FEKLASMSLI NL +YL T YNL  V  
Sbjct: 1   MDVESSSPSSHALIKKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFL 60

Query: 66  AYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPC 125
             V  IW G+ N   LAGAF++DAYLGRF+TLL GSIASF+GMG+  L A LP +RP  C
Sbjct: 61  VNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDAC 120

Query: 126 TTGESNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLN 185
               +   QP  WQL  L+ G GLL +GAGG+RPCNI+FGADQFDT+T+KG+A LE+F N
Sbjct: 121 IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFN 180

Query: 186 WWYFLFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSV 245
           WWYF F++AL+IALT VVY+QTNISW +GF IPTA    S+  F+ G+  YIC   +GSV
Sbjct: 181 WWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSV 240

Query: 246 FADMAKVIVATCHKRGIPKPSPDDLH--SPPMDSDSSKLAHTDRFLVFDKAATVVDSATE 305
           FAD+ KV+ A C KR + KP  D      P  D   + L      L F   A++V +  E
Sbjct: 241 FADIVKVVTAACKKRKV-KPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNPNE 300

Query: 306 LDEEGRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSL 365
           L+E+G +K +WRLCSV QV+  KC+  I+P+W  GIACFI   Q + +GILQA+QM+++ 
Sbjct: 301 LNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTF 360

Query: 366 GPH-FQIPPAWMSLTPMIALSIWIYIYE-KYVEFMKRKTHHNKRLSMKRRIDIGIVMSVL 425
           GPH FQ+P  WM+L  MI L+IWI +YE   +  +K+ T   KRL++K RI+  IVM ++
Sbjct: 361 GPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGII 420

Query: 426 CMVVAGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPES 485
           CM+VAG  EK RR SAL+N SFVSP+ + +L+P+F L+GLTEAF+A+A+ME LT  +PE 
Sbjct: 421 CMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEH 480

Query: 486 LRTVAGAIFFLSLSLASYLSSVIMGILSKISKK--SPWLGGNDLNKNRLDYFFYVVAVIA 545
           +R VAGAIFFLS S+ASY+ ++++ ++  +++K    WLG  DLNKNRL+ +F+++A I 
Sbjct: 481 MRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEGKSWLGDKDLNKNRLENYFFIIAGIQ 540

Query: 546 GLNFFYFRLVAAKF 551
             N  YFRL A+++
Sbjct: 541 VANLLYFRLFASRY 551

BLAST of Lag0012457 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 422.2 bits (1084), Expect = 6.9e-118
Identity = 228/569 (40.07%), Postives = 358/569 (62.92%), Query Frame = 0

Query: 21  GGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHSAYVFQIWGGTTNFAPLAG 80
           GGWRAV FIL NET E+L S+ L+AN ++YL  +++L+ V +A V  IW G TN  PL G
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 81  AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGES-NCPQPHPWQLAF 140
           A+++D Y+GRF T+ F S A+ LG+  +TL A  PQ+ P  C + +  +C  P+  Q+  
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

Query: 141 LYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLV 200
           L LG   L VG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  F++ LII  T+V
Sbjct: 173 LLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVV 232

Query: 201 VYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGI 260
           VY+Q  +SW +GF+IPT     +V++F +G + Y+   P+GS+F+ +A+VIVA   KR +
Sbjct: 233 VYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKL 292

Query: 261 PKPSPDD----LHSPPMDSD-SSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNEWRLC 320
             P+ DD     + P + S   SKL  +++F   DKAA V++   +L  EG   ++WRLC
Sbjct: 293 KLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPEGPPADKWRLC 352

Query: 321 SVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSLGPHFQIPPAWMSLT 380
           SV +VE+ KCL+ IVPIW+AGI    +M    +F + QA++M+R+LGP F+IP   +S+ 
Sbjct: 353 SVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVI 412

Query: 381 PMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEKVRRDS 440
            ++ + I++  Y++ +V FM+R T H   +++ +RI  GIV ++  M+VAGI+E++RR  
Sbjct: 413 SLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIR 472

Query: 441 ALE--NKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSL 500
           ++   + + ++P+ V  L P+ +L GL EAF  I  +E   +  PE +R++A ++F LS 
Sbjct: 473 SINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSF 532

Query: 501 SLASYLSSVIMGILSKIS---KKSPWLGGNDLNKNRLDYFFYVVAVIAGLNFFYFRLVAA 560
           + +SYLSS ++ ++ K S    +  WL  N LN  +LDYF+Y++AV+  +N  YF   A 
Sbjct: 533 AGSSYLSSFLVTVVHKFSGGHDRPDWLNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYCAR 592

Query: 561 KF-----LPKGDVDPRQKAGERQLEDEES 573
            +     LP  D +  + + + ++  ++S
Sbjct: 593 GYRYKVGLPIEDFEEDKSSDDVEMTSKKS 618

BLAST of Lag0012457 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 421.4 bits (1082), Expect = 1.2e-117
Identity = 236/566 (41.70%), Postives = 351/566 (62.01%), Query Frame = 0

Query: 21  GGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHSAYVFQIWGGTTNFAPLAG 80
           GGWRA+ FIL NET EKL S+ + AN +LYL  +++++ V +  V+ +W G TNFAPL G
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 81  AFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGE-SNCPQPHPWQLAF 140
           A ++DAY+GRF T+ + S+ S LG+  +TL A LPQ+ PPPC       C  P+  QL  
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

Query: 141 LYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIALTLV 200
           L+LG G L +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ LI + T+V
Sbjct: 136 LFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVV 195

Query: 201 VYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCHKRGI 260
           VY+QT +SW +GF+IPT+    +V++F  G RFY+   P+GSVF+ +A+VIVA   KR +
Sbjct: 196 VYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL 255

Query: 261 PKPSPDD----LHSPPMDSD-SSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNEWRLC 320
                DD     + PP+     SKL  TD+F   DKAA ++D   +L  EG   N+WRLC
Sbjct: 256 KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLC 315

Query: 321 SVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSLGPHFQIPPAWMSLT 380
           S+ +VE+ KCL+ +VP+W+AGI   ++M   ++F + QA +M+R +GPHF+IP A +++ 
Sbjct: 316 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVI 375

Query: 381 PMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEKVRRDS 440
             I + IW+ IYE   V F+ R      R+++ +R+ IGIV ++L M  AG +E VRR  
Sbjct: 376 SYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTR 435

Query: 441 ALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSL 500
           A E    ++ + V  L    +L GL E+F  I ++E   +  PE +R++A ++F LS + 
Sbjct: 436 ATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAA 495

Query: 501 ASYLSSVIMGILSKIS--KKSP-WLGGNDLNKNRLDYFFYVVAVIAGLNFFYFRLVAAKF 560
           A+YLSS+++  + K+S  K  P WL   DL++ +LDYF+Y++AV+  +N  YF   A ++
Sbjct: 496 ANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCAHRY 555

Query: 561 LPKGDVDPRQKAGERQLEDEESGYSH 577
             K          E+ L D E    H
Sbjct: 556 QYKAGSQIEDFNEEKSLLDIEPNQRH 571

BLAST of Lag0012457 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 381.3 bits (978), Expect = 1.3e-105
Identity = 217/561 (38.68%), Postives = 337/561 (60.07%), Query Frame = 0

Query: 17  KSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHSAYVFQIWGGTTNFA 76
           K  + GW+ + FI+ NETFEKL  +  ++NL++YL  ++NL ++ +A +   + GT NF 
Sbjct: 41  KVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFG 100

Query: 77  PLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPC-TTGESNCPQPHPW 136
               AFL D Y GR+ TL    IA FLG  V+ L A +PQ+ P  C T  +S C  P   
Sbjct: 101 TFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGG 160

Query: 137 QLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALIIA 196
           Q+AFL +G G LVVGAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ A I++
Sbjct: 161 QIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILS 220

Query: 197 LTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSVFADMAKVIVATCH 256
           LTLVVYVQ+N+SWT+G  IP      + L+F +G + Y+     GS  A +A+VI     
Sbjct: 221 LTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIK 280

Query: 257 KRGIPKPSPDDL-----HSPPMDSDSSKLAHTDRFLVFDKAATVVDSATELDEEGRSKNE 316
           KRG+ KP+         + PP  + +SKL +TD+F   DKAA ++    +L  +G+  + 
Sbjct: 281 KRGL-KPAKQPWLNLYNYYPPKYA-NSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADP 340

Query: 317 WRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSLGP-HFQIPPA 376
           W+LC++ QVE+ KC+V ++PIW A    ++++ Q  ++ + QA+Q +R LG   F IP A
Sbjct: 341 WKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAA 400

Query: 377 WMSLTPMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVVAGILEK 436
              +  M  ++++I +Y++  V  M+R T  +  +++ +RI  GI  +   +VVAG +E+
Sbjct: 401 TYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEE 460

Query: 437 VRRDSAL--------ENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLR 496
            RR  AL          K  +S +    LIP+  L+G+ EAFAAI  ME      PE++R
Sbjct: 461 RRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMR 520

Query: 497 TVAGAIFFLSLSLASYLSSVIMGILSKISKKSPWLGGN----DLNKNRLDYFFYVVAVIA 556
           + AG+IF++   ++SYL S ++  + + ++ S   GGN    DLNK RLD F++++A I 
Sbjct: 521 SFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSS--GGNWLAEDLNKGRLDLFYFMIAGIL 580

Query: 557 GLNFFYFRLVAAKFLPKGDVD 558
            +NF YF +++  +  KG  D
Sbjct: 581 AVNFAYFLVMSRWYRYKGSDD 596

BLAST of Lag0012457 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 375.2 bits (962), Expect = 9.7e-104
Identity = 209/550 (38.00%), Postives = 334/550 (60.73%), Query Frame = 0

Query: 9   SSSDPPPSKSAHGGWRAVRFILANETFEKLASMSLIANLVLYLHTMYNLDNVHSAYVFQI 68
           SSSD    +    GW+A+ +I+ NET E+LA+  L+AN ++Y+   Y++D V +  +   
Sbjct: 8   SSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINT 67

Query: 69  WGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAGLPQMRPPPCTTGE-- 128
           W   TNFAP+ GAF++D+Y G+F T++FGSIA  LGM VLT  + +P +RPPPCT  +  
Sbjct: 68  WSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQIT 127

Query: 129 SNCPQPHPWQLAFLYLGFGLLVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYF 188
             C      QL  L  G  LL VG GGIR C++ F  DQFD +TE+GR    SF +W+Y 
Sbjct: 128 GQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYT 187

Query: 189 LFSIALIIALTLVVYVQTNISWTLGFAIPTAAFIFSVLVFLSGRRFYICKAPQGSVFADM 248
             +I  ++++TLV+YVQ NISW +GFAIPT    F++L+   G R+Y+   P+GSVF+ +
Sbjct: 188 THTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGV 247

Query: 249 AKVIVATCHKRGIPKPSPDDLHSPPMDSD--SSKLAHTDRFLVFDKAATVVDSATELDEE 308
            KV+VA   KR     S  D H P +++D  S+KL  TD+F   +KA  V+++    DE 
Sbjct: 248 FKVLVAAYKKRKARFTSGIDYHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNN----DEA 307

Query: 309 GRSKNEWRLCSVHQVEQFKCLVGIVPIWAAGIACFISMQQMSSFGILQAIQMNRSL-GPH 368
           G    EWR C+V Q+E  K ++ I+PI+A+ I  F++M Q  +F + QA++M+    G  
Sbjct: 308 G--NEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTS 367

Query: 369 FQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKRKTHHNKRLSMKRRIDIGIVMSVLCMVV 428
           + IPPA +++  ++ + IW+  YE   V  ++  T  N  +S+ +++ IG + S+  M++
Sbjct: 368 YLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLI 427

Query: 429 AGILEKVRRDSALENKSFVSPLHVSVLIPEFVLSGLTEAFAAIAVMELLTTHLPESLRTV 488
           +GI+E+ RRD +L        + V  L P+ VL G  + F  + + E     +P ++R++
Sbjct: 428 SGIVERKRRDLSLNGVK----MSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSI 487

Query: 489 AGAIFFLSLSLASYLSSVIMGILSKISKK--SPWLGGNDLNKNRLDYFFYVVAVIAGLNF 548
             ++ +L LSLASYLSS ++ I+  ++ +    WL  +D++K++LD F+Y +A ++ LNF
Sbjct: 488 GNSLLYLGLSLASYLSSAMVSIVHSVTARGGQSWL-TDDIDKSKLDCFYYFIAALSTLNF 546

Query: 549 FYFRLVAAKF 551
            +F   A ++
Sbjct: 548 IFFFWCARRY 546